Rroxscaffold_7G00178380

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009147
Physical Location & Seq
Reverse (-)
18087829 .. 18097818
9990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00178380.1

Sequence Viewer

Length: 6222 bp
ATGCACACACATGGAAAGGAAACGAAAAGACTGGGACTCGCTGCTCTCTGTGTCACTCTGTCAGCCCTCCATTTTTCACTACTCTCTCTGATATCTCCGCCGCTGCTCCACTCTCATTCCGGTCATTCTCCGGCACTGAATTCCCTCCATGTCAAGGTGGCCAAGGAGAAATTCAAAAGAGGCGTATACTGCAAATGTCAAAGTCTTCCTCCCCTGAACTACGGGAACCCGTGTCTTCTTCTACTTCTCGGTCTAGTTCATGTTACACCCAGGGATTTAGATGATACAAAAATGGATGATGAAGAAGGTACAATGGCAAGTGTTGCTGAATTTGTCGAGAAACTACATGCTGGCATGTCTTCACCGCATGAAAAAGAACTTATTACAGCACGTCTGCTAGGTATTGCCAAAGCAAGGAAGGATGCAAGAACAATTATAGGTTCACATTCCCAGGCTATGCCGTTGTTCATAAATATTCTGAGGAGTGGCACCCCTATTGCAAAGGTTAATGTTGCGGCAACTCTGAGTGTCCTGTGCAAAGATGAAGACCTGCGTCTTAAAGTACTTCTAGATGATCACGTTGGCATAAAAATATTTATTACAGAAGGTGTTGTACCAAGCTTGTGGAATCAGCTCAATCCAAACAGCAACCAGGACAAAGTGGTAGAGGGATTTGTGACAGGGGCTTTAAGAAATCTCTGTGGTGACAAGGATGGCTACTGGAGAGCAACACTTGAGGCTGGTGGAGTAGATATCACTGTGGGTCTCCTGTATTCTGACAATGTGGCTGCCCAGTCCAATGCTGCGTCCCTTTTGGCCCGTCTCATGTTGGCTTTCAGTGATAGCATCCCCAAAGTAATAGATTCTGGAGCTGTCAAAGCTTTGCTTCGGCTCGTGGGTCAAGAAAATAACGTTTCTGTTCGTGCTAGCGCTGCTGATGCTCTGGAAGCTCTTTCTTCAAAGTCAACTGGCGCTAAGAAAGCTATTGTTGATGCAAATGGTCTTCCAGTTCTTATTGGAGCTATTGTTGCTCCATCTAAGGAGTGCATGCAAGGTGAGTGCGGGCAGGCTCTCCAGGAGCATTCAACAAGGGCTTTAGCAAATATTTGTGGTGGGGTGTCTGCTTTAATACTTTATCTTGGAGAGCTTTCACAGTCAGCTCGCCTAGCTGCCCCAGTTGCTGATATTATTGGAGCTCTTGCATACACTTTAATGGTCTTTGAGCAAAAATCTGGTGATGGTAAGGAATCTTTTAAGGTAACGAAGATAGAGGATATTCTTGTAATGCTGCTAAAGCCTCGTGATAATAAGCTTGTTCAAGAGCGTGTCCTTGAGGCCATGGCTAGTCTATATGGCAACATTCATCTCTCAAAATGGCTCAATCATGCAGAAGCGAAGAAGGTGCTTATTGGACTTATAACAATGGCTGCTGCTGATGTACAAGAGTCTCTGATACTCTCTCTGACAAGCTTATGCTGTGATGGTGTTGGAATCTGGGAGTCCATTGGTGAGAGAGAAGGAATTCAGCTACTTATTTCATTGCTTGGCTTATCCAGTGAGCAGCATCAAGAATATGCTGTTCAGTTGCTAGGGATCTTAACTGACCAGGTTGATGACAGCAAGTGGGCTATTACTGCTGCTGGTGGGATTCCTCCTCTAGTGCAGTTGTTAGAGACGGGATCGCAAAAGGCGAAGGAGGACGCTGCTCATGTGTTGTGGAACTTGTGCTGTCACAGTGAGGATATTCGTGCATGTGTTGAAAGTGCAGGAGCCATTCCAGCATTTTTATGGCTTCTAAAAAGTGGTGACTCTCCATGCTCAAAGGCCCATACCGTCAGGGTGTTGGGTCATGTACTCACAATGGCATCACACAAGGATCTTGTGCATAAGGGTTCAGCATCTAATAAAGGGTTGAGGTCTCTCGTTCAGGTTCTCAACTCGTCAAATGAAGAAACTCAAGAGTATGCCGCATCTGTCTTAGCTGATCTATTCAGCATGAGACAAGATATTTGTGACACTCTAGCAACTGATGAGATCGTGCATCCTTGCATGAAGCTTTTGACCAGCAGCACACAAGCTGTTGCAACACAGTCGGCTCGAGCTTTGGGTGCTCTTTCCCGTCCTATGAAGACCAAAACAATAAGCAAGATGTCTTATATTGCAGAAGGGGATGTTAAACCCCTCATCAAACTAGCAAAAACTTCTTCAATTGATGCTGCTCAAACTGCAGTTGCTGCACTCGCCAATCTTCTCTCTGATCCCCAGATCGCTGCAGAAGCCCTGGCAGAAGATGTTGTTTCAGCTTTGATAAGAGTACTGGAAGATGGAACTACAGAAGGTAAAAAGAATGCATCCCGTGCCCTTCATCAATTGTTGAAGCATTTTCCAGTAGGTGATGTGCTCACAGGAAGTGATCATTGCCATTTTGCTATTCGTGCTGTTGTTGATTCCTTAAATGCATTGGATATGGATGAGATTGATGCTGCTGATGCTTTAGAAGTAGTAGCACTTTTGGCCAGAACCAAACAGGGTGCGAACTTCACTTACTCCCCATGGTTTGTATTTGCCGAAGTTGAATCGAGCTTAGAACCTCTTGTCCGCTGTCTGGTCGAAGGGTCTCCTTTGCTTCAGGATAAGGCAATAGAAGTTTTGTCTAGGCTTTGCGGGGAGCAACCAGTTGTGCTTGGTGATCTATTGATTGCAAGATCTGGATCCCTTGGTTCACTAGCTGACAGAATAATGAACTCATCTAGTTTAGAAGTCAGAGTTGGAGGAGCTGCATTACTCATTTGTGCAGCAAAAGAGCACAAAGAGCAGTGGATGGAAGTACTTGAGGTGTCAGGATATTTAAAACCATTAATGTATGCTTTAGTGGATATGATGAAGCAAAATTGTAGCTGCTCCTCTTTAGAAATTGAAGTCCGAACTTCTAAAGCTTTTATGGAAAGAACTGCATTCCGCGAAGTGGATGAGTTTGATAGCAAACTCACCATTATGGAAGCTGGAGGTCTTGAGGCTCTCTCTGACAAGCTTGAGAGCCACACTTCCAATCCACAGGCAGATTATGAGGATACTGAAGGTATATGGATTAGTGCACTGCTTCTGGCTATTCTCTTCCAAGATGCCAATGTCGTTTCATCTCCTACAACAATGCGGATCATAGCTTCTCTTGCGCTTCTGCTGAGATCAGATGAGGTGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCAGTCTTGTTTGCAGTGGTAATAAGGGAATAATTCTTGCCATTGCAAATTCAGGTGCTGTTGCTGGATTGATAACCCTCATTGGTTACGTAGAATCTGATATGCCTAACCTTGTTACTTTATCAGAAGAGTTTTCTCTGGTGCGAAACCCTGATCAAGTTGTTTTGGAATACCTTTTTGATTTTGAAGATGTAAGAGTTGGATCCACAGCACGCAAATCTATACCTCTCTTGGTGGATCTCTTGAGACCAATGCCAGATAGACCTGGTGCCCCTCCAGTTGCTCTTAAACTCTTGACCTGTATTGCTGATGGAAGTGATACAAACAAATTAGTCATGGCTGAAGCTGGAGCTCTGGACGCTCTGACAAAGTACCTGTCTTTGAGCCCACAAGATTCGACTGAAGCTGCAATATCTGATTTATTCAGAATATTGTTTAGCCATCCTGATCTGATTCGCTATGAAGCATCAGTTAGTTCGTTGAATCAGCTCATAGCTGTTCTGCGTCTGGGGTCAAGAAATGCTAGATACAGTGCTGCAAGAGCTCTTCATGAACTATTTGATGCTGAGAACATCAGAGATTCTGATCTAGCCAGGCAATCTGTTCAGCCATTGGTTGACATGCTTAACGCTGCATCAGAGAATGAACAAGAGGCTGCTCTTGTTGCGGTAATTAAGCTGACTTCCGGAAATTCTTCTACCGCAGCTTTGTTGACTGATGTGGAGGGAAATCCACTGGAGAGTTTATTTAAAATATTGTCTTCTGCTTCATCCTTGGATTTGAAGAGACTTGCTGCACAACTATGTTGTGTACTATTTGATAGTACCAAAGTCAGAGGAAATCCAATTGCCTCTGAATGTATAGAGCCTCTTATTTCCTTGATGCTTTCTGGTATAAATGCAGCTGTAGAAGCTGGGGTTTGTGCCTTTGAAAAATTGTTGGATGATGAACACCAGGTAGAGCTTGCAGTGAACTATAATGTTGTCGATCTTCTTGTTGGATTAGTTTCTGGTACGAACAGTCAGCTCATTGAGGCTAGCATCTGCTCTCTCATAAAGTTGGGGAAAGACCGGACCCAATGCAAATCGGATATGATCAATGCTGGCATTATTGATAACTGTCTTGAGCTACTCCCTGTTGCAGCCAGTTCATTATGCTCTTCTATTGCTGAATTATTTCGCATTTTGACAAATAGTGATGCAATTGCTAGAAGTTCAGCTGCTGCAACAATTGTAGAACCCCTTTTCCTAGTTTTACTTCGTCCAGATTTCAGTATGTGGGGACAGCACAGTGCATTGCAAGCACTAGTAAATATTTTGGAGAAGCCTCAAAGCCTTGCCACTTTAAAACTTACGCCTAGCCAAGTCATTGAGCCTCTGATTTCATTTCTTGAATCCCCATCTCTAGCCATCCAGCAGCTTGGTACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCAAGATATTACAACAAAAAATGCTGTTGTTCCTCTTGTGCAACTTGCGGGAATTGGAATACTGAACTTACAGCAAACAGCGATAAAGGCATTGGAAAAAATATCCACAAGATGGCCAAAGGCAGTTGCTGATGCTGGAGGTATTTTTGAGCTTGGAAAGGTTATTATACAAGATGATCCTCAGCCACCTCATGCATTGTGGGAATCAGCTGCTTTAGTTCTCTCAAATGTTTTGTGCTTCAATGCTGAGTACTACTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACAGTGGATAGCACTATTACGGTGGCTCTCAATGCACTACTTGTTCATGAAAGGAGTGATAAGCTAAGTGCTGAACAGATGACTGAAAATGGTGCTATAGATGCCTTGTTGGACCTTTTAACATCTCATCAGTGTGAAGAAGCATCTGGAAGGTTACTTGAAGCGTTATTCAACAATGTCAGGATACGAGCAATGAAGGTTTCCAAGTATGCAATAGCACCTTTATCACAGTATCTGTTGGATTCACAAACCAAATCACAATCTGGAAAACTTCTCGTAGCTCTAGCTCTTGGAGACCTTTCCCAACACGAAGGGCTTGCTAGAGCCAGTGATTCTGTTTCTGCATGTCGGGCATTAATAAGCTTGCTTGAAGATCAGCCCACAGAAGATATGAAAATGAAGTTGCTGGGCAAAGCAGCATTGCTTATCAAATTTTTGTTTTCTAATCACACGCTTCAAGAATATGTGTCAAACGAGCTCATCAGATCTTTGACAGCTGCATTAGAGAGAGAGTTGTGGTCCACAGCAACTATTAATGAAGAGGTTTTGAGAGCCCTACATATGATATTCACCAACTTTCCTAAGCTCCATATTTCCGAGGCAGCCACTCTCTGCATTCCCAATCTGATTGGAGCCCTTAAATCTGGCAGTGAGGCAGCTCAGGATGTCGTGTTGGACACCCTTTGCTTGTTGAGACATTCTTGGTCAACCATGCCAATAGATATTGCAAAGTCTCAATCCGTGGTTGCTGCTGAAGCCATTCCCATCTTACAAATGCTTATGAAAACCTGCCCACCAAGTTTCCATGAGAGAGCAGACAGCCTGTTGCATTGCTTACCTGGTTGTTTGACTGTTACAATCAAGCGTGGAAACAACCTTAAACAGGCTATGGGAGGCACAAATGCTTTTTGCCGATTGACAATAGGCAACGGTCCTCCTCGACAAACCAAGGTAGTGAGCCACTGCACTTCTCCTGAATGGAAAGAAGGGTTTACGTGGGCATTTGATGTGCCCCCAAAGGGACAAAAGCTCCACATCATCTGCAAAAGCAAAAATACCTTTGGAAAGTCAACACTGGGGAGAGTGACTATCCAGATCGATAAAGTTGTGTCAGAAGGAGTATACAGTGGATTATTCAGCCTAAACCATGACAACAGCAAAGATGGATCTTCACGTACACTTGAAATCGAGATTATCTGGTCCAACAGAATGCCCGATGATGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2073

Amino Acids

223.45

Weight (kDa)

5.44

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 415 - 653 2.1e-09 ARMC5
Arm PF00514 539 - 576 2.5e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 634 - 788 4.6e-08 ARMC5
C2 PF00168 1946 - 2044 8.8e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
Acc36I ACCTGC 2 cut(s) 558, 5789
AccB1I GGYRCC 2 cut(s) 488, 3497
AccB7I CCANNNNNTGG 2 cut(s) 3226, 4806
AccI GTMKAC 2 cut(s) 186, 6114
AccII CGCG 1 cut(s) 2961
AccIII TCCGGA 1 cut(s) 3914
AclI AACGTT 1 cut(s) 912
AcoI YGGCCR 4 cut(s) 159, 2514, 3228, 4808
AcsI RAATTY 7 cut(s) 139, 170, 329, 1521, 3277, 3919, 5423
AcuI CTGAAG 5 cut(s) 2612, 3096, 3591, 3651, 5766
AfeI AGCGCT 1 cut(s) 931
AfiI CCNNNNNNNGG 8 cut(s) 154, 414, 2605, 2965, 3226, 4806, 5875, 6011
AhlI ACTAGT 1 cut(s) 4534
AjiI CACGTC 1 cut(s) 392
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 2751, 2783
AloI GAACNNNNNNTCC 4 cut(s) 2580, 2612, 4458, 4490
Alw21I GWGCWC 8 cut(s) 1198, 2113, 2403, 2808, 3097, 3583, 3775, 5472
Alw44I GTGCAC 1 cut(s) 3093
AlwNI CAGNNNCTG 6 cut(s) 1181, 2234, 3287, 4451, 4823, 5227
Ama87I CYCGRG 1 cut(s) 2097
Aor13HI TCCGGA 1 cut(s) 3914
Aor51HI AGCGCT 1 cut(s) 931
AoxI GGCC 7 cut(s) 159, 816, 1335, 1824, 2514, 3228, 4808
ApaLI GTGCAC 1 cut(s) 3093
ApoI RAATTY 7 cut(s) 139, 170, 329, 1521, 3277, 3919, 5423
ArsI GACNNNNNNTTYG 4 cut(s) 1992, 2024, 3590, 3622
AseI ATTAAT 3 cut(s) 2858, 5348, 5526
Asp700I GAANNNNTTC 5 cut(s) 1521, 3922, 4404, 4689, 5751
AspLEI GCGC 3 cut(s) 932, 974, 3175
AspS9I GGNCC 7 cut(s) 817, 1825, 4302, 5104, 5511, 5924, 6192
AsuNHI GCTAGC 2 cut(s) 926, 4265
AvaI CYCGRG 1 cut(s) 2097
AvaII GGWCC 5 cut(s) 4302, 5104, 5511, 5924, 6192
BaeGI GKGCMC 4 cut(s) 2362, 3097, 3502, 6006
BalI TGGCCA 4 cut(s) 161, 2516, 3230, 4810
BamHI GGATCC 2 cut(s) 2711, 3431
BanI GGYRCC 2 cut(s) 488, 3497
BanII GRGCYC 7 cut(s) 1198, 3583, 3617, 3775, 5472, 5548, 5629
BarI GAAGNNNNNNTAC 2 cut(s) 597, 629
BauI CACGAG 2 cut(s) 893, 1299
BbsI GAAGAC 7 cut(s) 197, 227, 351, 552, 995, 2135, 3981
Bbv12I GWGCWC 8 cut(s) 1198, 2113, 2403, 2808, 3097, 3583, 3775, 5472
BbvCI CCTCAGC 1 cut(s) 4875
BceAI ACGGC 1 cut(s) 445
BcgI CGANNNNNNTGC 6 cut(s) 1384, 1418, 2073, 2107, 5291, 5325
BciVI GTATCC 2 cut(s) 3064, 5169
BclI TGATCA 4 cut(s) 574, 2413, 3382, 4323
BcuI ACTAGT 1 cut(s) 4534
BfmI CTRYAG 5 cut(s) 2226, 2271, 2331, 4134, 5088
BfoI RGCGCY 2 cut(s) 933, 975
BfuAI ACCTGC 2 cut(s) 558, 5789
BfuI GTATCC 2 cut(s) 3064, 5169
BglII AGATCT 2 cut(s) 2705, 5477
BmcAI AGTACT 4 cut(s) 564, 2316, 2829, 4946
Bme18I GGWCC 5 cut(s) 4302, 5104, 5511, 5924, 6192
BmeT110I CYCGRG 1 cut(s) 2097
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 7 cut(s) 817, 1825, 4302, 5104, 5511, 5924, 6192
BmiI GGNNCC 9 cut(s) 227, 490, 1771, 2713, 3433, 3499, 4304, 4959, 5626
BmrI ACTGGG 5 cut(s) 41, 787, 1169, 3214, 6077
BmtI GCTAGC 2 cut(s) 930, 4269
BmuI ACTGGG 5 cut(s) 41, 787, 1169, 3214, 6077
BoxI GACNNNNGTC 1 cut(s) 552
BpiI GAAGAC 7 cut(s) 197, 227, 351, 552, 995, 2135, 3981
BplI GAGNNNNNCTC 2 cut(s) 3005, 3037
BpmI CTGGAG 8 cut(s) 742, 888, 1058, 3024, 3489, 3597, 3986, 4851
Bpu10I CCTNAGC 3 cut(s) 4875, 5574, 5652
BpuEI CTTGAG 8 cut(s) 755, 1352, 1941, 2852, 3032, 3053, 3493, 4373
Bsa29I ATCGAT 1 cut(s) 6090
BsaAI YACGTR 3 cut(s) 3319, 5988, 6167
BsaBI GATNNNNATC 2 cut(s) 2710, 3813
BsaI GGTCTC 5 cut(s) 770, 1923, 2622, 3469, 5280
BsaWI WCCGGW 3 cut(s) 119, 3914, 4299
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 6006, 6036
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 414, 2605, 2965, 3226, 4806, 5875, 6011
Bse3DI GCAATG 7 cut(s) 1538, 2416, 3270, 4523, 5190, 5411, 5821
Bse8I GATNNNNATC 2 cut(s) 2710, 3813
BseAI TCCGGA 1 cut(s) 3914
BseCI ATCGAT 1 cut(s) 6090
BseJI GATNNNNATC 2 cut(s) 2710, 3813
BseLI CCNNNNNNNGG 8 cut(s) 154, 414, 2605, 2965, 3226, 4806, 5875, 6011
BseMI GCAATG 7 cut(s) 1538, 2416, 3270, 4523, 5190, 5411, 5821
BseMII CTCAG 7 cut(s) 470, 515, 3173, 3786, 4889, 4932, 5666
BseRI GAGGAG 5 cut(s) 496, 1644, 2787, 2893, 5919
BseSI GKGCMC 4 cut(s) 2362, 3097, 3502, 6006
BseYI CCCAGC 2 cut(s) 4142, 5398
BsgI GTGCAG 6 cut(s) 1682, 1785, 2220, 2814, 4008, 5941
Bsh1236I CGCG 1 cut(s) 2961
BshFI GGCC 7 cut(s) 161, 818, 1337, 1826, 2516, 3230, 4810
BshNI GGYRCC 2 cut(s) 488, 3497
BshVI ATCGAT 1 cut(s) 6090
BsiHKAI GWGCWC 8 cut(s) 1198, 2113, 2403, 2808, 3097, 3583, 3775, 5472
BsiHKCI CYCGRG 1 cut(s) 2097
BsiSI CCGG 4 cut(s) 120, 131, 3915, 4300
BslFI GGGAC 4 cut(s) 48, 793, 4524, 6027
BslI CCNNNNNNNGG 8 cut(s) 154, 414, 2605, 2965, 3226, 4806, 5875, 6011
BsmBI CGTCTC 2 cut(s) 827, 1667
BsmFI GGGAC 4 cut(s) 48, 793, 4524, 6027
BsmI GAATGC 6 cut(s) 1081, 2353, 2954, 4987, 5607, 6207
BsnI GGCC 7 cut(s) 161, 818, 1337, 1826, 2516, 3230, 4810
Bso31I GGTCTC 5 cut(s) 770, 1923, 2622, 3469, 5280
BsoBI CYCGRG 1 cut(s) 2097
Bsp13I TCCGGA 1 cut(s) 3914
Bsp1407I TGTACA 1 cut(s) 1438
Bsp19I CCATGG 2 cut(s) 1338, 2552
BspANI GGCC 7 cut(s) 161, 818, 1337, 1826, 2516, 3230, 4810
BspCNI CTCAG 7 cut(s) 471, 516, 3174, 3787, 4888, 4933, 5665
BspDI ATCGAT 1 cut(s) 6090
BspEI TCCGGA 1 cut(s) 3914
BspFNI CGCG 1 cut(s) 2961
BspHI TCATGA 2 cut(s) 3778, 5038
BspLI GGNNCC 9 cut(s) 227, 490, 1771, 2713, 3433, 3499, 4304, 4959, 5626
BspMAI CTGCAG 2 cut(s) 2230, 2275
BspMI ACCTGC 2 cut(s) 558, 5789
BspOI GCTAGC 2 cut(s) 930, 4269
BspQI GCTCTTC 2 cut(s) 3780, 4393
BspT107I GGYRCC 2 cut(s) 488, 3497
BspTNI GGTCTC 5 cut(s) 770, 1923, 2622, 3469, 5280
BsrDI GCAATG 7 cut(s) 1538, 2416, 3270, 4523, 5190, 5411, 5821
BsrGI TGTACA 1 cut(s) 1438
BssNAI GTATAC 2 cut(s) 187, 6115
BssSI CACGAG 2 cut(s) 893, 1299
BssT1I CCWWGG 6 cut(s) 162, 1338, 2552, 2716, 4002, 5940
Bst1107I GTATAC 2 cut(s) 187, 6115
Bst2BI CACGAG 2 cut(s) 893, 1299
Bst6I CTCTTC 6 cut(s) 3119, 3351, 3780, 4007, 4393, 5526
BstAPI GCANNNNNTGC 3 cut(s) 323, 2234, 2357
BstAUI TGTACA 1 cut(s) 1438
BstBAI YACGTR 3 cut(s) 3319, 5988, 6167
BstDSI CCRYGG 3 cut(s) 1338, 2552, 5733
BstENI CCTNNNNNAGG 1 cut(s) 5873
BstFNI CGCG 1 cut(s) 2961
BstH2I RGCGCY 2 cut(s) 933, 975
BstHHI GCGC 3 cut(s) 932, 974, 3175
BstNSI RCATGY 6 cut(s) 350, 358, 1051, 1755, 3853, 5340
BstPAI GACNNNNGTC 1 cut(s) 552
BstSFI CTRYAG 5 cut(s) 2226, 2271, 2331, 4134, 5088
BstSLI GKGCMC 4 cut(s) 2362, 3097, 3502, 6006
BstSNI TACGTA 1 cut(s) 3319
BstUI CGCG 1 cut(s) 2961
BstV2I GAAGAC 7 cut(s) 197, 227, 351, 552, 995, 2135, 3981
BstX2I RGATCY 8 cut(s) 1593, 1876, 2705, 2711, 3431, 3466, 5477, 6158
BstXI CCANNNNNNTGG 2 cut(s) 624, 4649
BstYI RGATCY 8 cut(s) 1593, 1876, 2705, 2711, 3431, 3466, 5477, 6158
BstZ17I GTATAC 2 cut(s) 187, 6115
Bsu15I ATCGAT 1 cut(s) 6090
BsuI GTATCC 2 cut(s) 3064, 5169
BsuRI GGCC 7 cut(s) 161, 818, 1337, 1826, 2516, 3230, 4810
BsuTUI ATCGAT 1 cut(s) 6090
BtgI CCRYGG 3 cut(s) 1338, 2552, 5733
BtrI CACGTC 1 cut(s) 392
BtsI GCAGTG 6 cut(s) 2822, 3095, 3250, 4203, 5647, 5953
BveI ACCTGC 2 cut(s) 558, 5789
CaiI CAGNNNCTG 6 cut(s) 1181, 2234, 3287, 4451, 4823, 5227
CciI TCATGA 2 cut(s) 3778, 5038
CfoI GCGC 3 cut(s) 932, 974, 3175
Cfr13I GGNCC 7 cut(s) 817, 1825, 4302, 5104, 5511, 5924, 6192
ClaI ATCGAT 1 cut(s) 6090
CseI GACGC 5 cut(s) 542, 795, 1709, 3596, 3722
CsiI ACCWGGT 4 cut(s) 1605, 3493, 4182, 5830
DraI TTTAAA 3 cut(s) 2850, 3979, 4575
EaeI YGGCCR 4 cut(s) 159, 2514, 3228, 4808
Eam1104I CTCTTC 6 cut(s) 3119, 3351, 3780, 4007, 4393, 5526
EarI CTCTTC 6 cut(s) 3119, 3351, 3780, 4007, 4393, 5526
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 4 cut(s) 1196, 3581, 3773, 5470
Eco105I TACGTA 1 cut(s) 3319
Eco130I CCWWGG 6 cut(s) 162, 1338, 2552, 2716, 4002, 5940
Eco24I GRGCYC 7 cut(s) 1198, 3583, 3617, 3775, 5472, 5548, 5629
Eco31I GGTCTC 5 cut(s) 770, 1923, 2622, 3469, 5280
Eco32I GATATC 2 cut(s) 93, 754
Eco47I GGWCC 5 cut(s) 4302, 5104, 5511, 5924, 6192
Eco47III AGCGCT 1 cut(s) 931
Eco53kI GAGCTC 4 cut(s) 1196, 3581, 3773, 5470
Eco57I CTGAAG 5 cut(s) 2612, 3096, 3591, 3651, 5766
Eco88I CYCGRG 1 cut(s) 2097
EcoICRI GAGCTC 4 cut(s) 1196, 3581, 3773, 5470
EcoNI CCTNNNNNAGG 1 cut(s) 5873
EcoRI GAATTC 2 cut(s) 139, 1521
EcoRV GATATC 2 cut(s) 93, 754
EcoT14I CCWWGG 6 cut(s) 162, 1338, 2552, 2716, 4002, 5940
EcoT22I ATGCAT 3 cut(s) 2353, 2461, 4891
EcoT38I GRGCYC 7 cut(s) 1198, 3583, 3617, 3775, 5472, 5548, 5629
ErhI CCWWGG 6 cut(s) 162, 1338, 2552, 2716, 4002, 5940
Esp3I CGTCTC 2 cut(s) 827, 1667
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 4 cut(s) 48, 793, 4524, 6027
FauI CCCGC 3 cut(s) 1055, 2657, 4735
FauNDI CATATG 1 cut(s) 5553
FbaI TGATCA 4 cut(s) 574, 2413, 3382, 4323
FblI GTMKAC 2 cut(s) 186, 6114
FriOI GRGCYC 7 cut(s) 1198, 3583, 3617, 3775, 5472, 5548, 5629
GlaI GCGC 3 cut(s) 931, 973, 3174
GsaI CCCAGC 2 cut(s) 4146, 5402
GsuI CTGGAG 8 cut(s) 742, 888, 1058, 3024, 3489, 3597, 3986, 4851
HaeII RGCGCY 2 cut(s) 933, 975
HaeIII GGCC 7 cut(s) 161, 818, 1337, 1826, 2516, 3230, 4810
HapII CCGG 4 cut(s) 120, 131, 3915, 4300
HgaI GACGC 5 cut(s) 542, 795, 1709, 3596, 3722
HhaI GCGC 3 cut(s) 932, 974, 3175
Hin6I GCGC 3 cut(s) 930, 972, 3173
HinP1I GCGC 3 cut(s) 930, 972, 3173
HincII GTYRAC 5 cut(s) 966, 3847, 3942, 5700, 6063
HindII GTYRAC 5 cut(s) 966, 3847, 3942, 5700, 6063
HindIII AAGCTT 8 cut(s) 619, 879, 1310, 1468, 2054, 2934, 3029, 5353
HpaII CCGG 4 cut(s) 120, 131, 3915, 4300
HpyCH4IV ACGT 6 cut(s) 391, 579, 912, 3318, 5987, 6166
HpySE526I ACGT 6 cut(s) 391, 579, 912, 3318, 5987, 6166
HspAI GCGC 3 cut(s) 930, 972, 3173
Kpn2I TCCGGA 1 cut(s) 3914
Ksp22I TGATCA 4 cut(s) 574, 2413, 3382, 4323
LguI GCTCTTC 2 cut(s) 3780, 4393
MabI ACCWGGT 4 cut(s) 1605, 3493, 4182, 5830
MaeII ACGT 6 cut(s) 391, 579, 912, 3318, 5987, 6166
MfeI CAATTG 5 cut(s) 2208, 2369, 4074, 4431, 4458
MflI RGATCY 8 cut(s) 1593, 1876, 2705, 2711, 3431, 3466, 5477, 6158
MlsI TGGCCA 4 cut(s) 161, 2516, 3230, 4810
MluNI TGGCCA 4 cut(s) 161, 2516, 3230, 4810
MlyI GAGTC 4 cut(s) 30, 1454, 1508, 1802
MmeI TCCRAC 9 cut(s) 1468, 2749, 3409, 4149, 4207, 5082, 5211, 5646, 6219
Mox20I TGGCCA 4 cut(s) 161, 2516, 3230, 4810
Mph1103I ATGCAT 3 cut(s) 2353, 2461, 4891
MroI TCCGGA 1 cut(s) 3914
MroXI GAANNNNTTC 5 cut(s) 1521, 3922, 4404, 4689, 5751
MscI TGGCCA 4 cut(s) 161, 2516, 3230, 4810
MslI CAYNNNNRTG 6 cut(s) 9, 1211, 1786, 2993, 4030, 5124
Msp20I TGGCCA 4 cut(s) 161, 2516, 3230, 4810
MspA1I CMGCKG 6 cut(s) 103, 2601, 4133, 4448, 4904, 5489
MspI CCGG 4 cut(s) 120, 131, 3915, 4300
MunI CAATTG 5 cut(s) 2208, 2369, 4074, 4431, 4458
Mva1269I GAATGC 6 cut(s) 1081, 2353, 2954, 4987, 5607, 6207
MvnI CGCG 1 cut(s) 2961
NcoI CCATGG 2 cut(s) 1338, 2552
NdeI CATATG 1 cut(s) 5553
NheI GCTAGC 2 cut(s) 926, 4265
NlaIV GGNNCC 9 cut(s) 227, 490, 1771, 2713, 3433, 3499, 4304, 4959, 5626
NmuCI GTSAC 7 cut(s) 52, 676, 704, 1730, 1805, 2012, 6076
NsiI ATGCAT 3 cut(s) 2353, 2461, 4891
NspI RCATGY 6 cut(s) 350, 358, 1051, 1755, 3853, 5340
PaeI GCATGC 1 cut(s) 1051
PaeR7I CTCGAG 1 cut(s) 2097
PagI TCATGA 2 cut(s) 3778, 5038
PasI CCCWGGG 1 cut(s) 270
PciSI GCTCTTC 2 cut(s) 3780, 4393
PcsI WCGNNNNNNNCGW 1 cut(s) 1688
PctI GAATGC 6 cut(s) 1081, 2353, 2954, 4987, 5607, 6207
PdmI GAANNNNTTC 5 cut(s) 1521, 3922, 4404, 4689, 5751
PflMI CCANNNNNTGG 2 cut(s) 3226, 4806
PfoI TCCNGGA 1 cut(s) 1074
PleI GAGTC 4 cut(s) 30, 1453, 1507, 1802
PpsI GAGTC 4 cut(s) 30, 1453, 1507, 1802
Ppu21I YACGTR 3 cut(s) 3319, 5988, 6167
PshAI GACNNNNGTC 1 cut(s) 552
PshBI ATTAAT 3 cut(s) 2858, 5348, 5526
PsiI TTATAA 1 cut(s) 1418
Psp124BI GAGCTC 4 cut(s) 1198, 3583, 3775, 5472
Psp1406I AACGTT 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 4142, 5398
PspN4I GGNNCC 9 cut(s) 227, 490, 1771, 2713, 3433, 3499, 4304, 4959, 5626
PspPI GGNCC 7 cut(s) 817, 1825, 4302, 5104, 5511, 5924, 6192
PspXI VCTCGAGB 1 cut(s) 2097
PsrI GAACNNNNNNTAC 2 cut(s) 2528, 2560
PstI CTGCAG 2 cut(s) 2230, 2275
PstNI CAGNNNCTG 6 cut(s) 1181, 2234, 3287, 4451, 4823, 5227
PsuI RGATCY 8 cut(s) 1593, 1876, 2705, 2711, 3431, 3466, 5477, 6158
PvuII CAGCTG 4 cut(s) 4133, 4448, 4904, 5489
RseI CAYNNNNRTG 6 cut(s) 9, 1211, 1786, 2993, 4030, 5124
SacI GAGCTC 4 cut(s) 1198, 3583, 3775, 5472
SapI GCTCTTC 2 cut(s) 3780, 4393
Sau96I GGNCC 7 cut(s) 817, 1825, 4302, 5104, 5511, 5924, 6192
ScaI AGTACT 4 cut(s) 564, 2316, 2829, 4946
SchI GAGTC 4 cut(s) 30, 1454, 1508, 1802
SexAI ACCWGGT 4 cut(s) 1605, 3493, 4182, 5830
SfcI CTRYAG 5 cut(s) 2226, 2271, 2331, 4134, 5088
Sfr274I CTCGAG 1 cut(s) 2097
SinI GGWCC 5 cut(s) 4302, 5104, 5511, 5924, 6192
SlaI CTCGAG 1 cut(s) 2097
SmiMI CAYNNNNRTG 6 cut(s) 9, 1211, 1786, 2993, 4030, 5124
SmlI CTYRAG 9 cut(s) 734, 1331, 1956, 2097, 2831, 3011, 3032, 3472, 4352
SmoI CTYRAG 9 cut(s) 734, 1331, 1956, 2097, 2831, 3011, 3032, 3472, 4352
SnaBI TACGTA 1 cut(s) 3319
SpeI ACTAGT 1 cut(s) 4534
SphI GCATGC 1 cut(s) 1051
SspI AATATT 6 cut(s) 475, 594, 1105, 3660, 3984, 4543
SstI GAGCTC 4 cut(s) 1198, 3583, 3775, 5472
StyI CCWWGG 6 cut(s) 162, 1338, 2552, 2716, 4002, 5940
TaiI ACGT 6 cut(s) 394, 582, 915, 3321, 5990, 6169
TaqI TCGA 9 cut(s) 336, 2098, 2579, 2610, 3626, 4215, 5932, 6090, 6180
TaqII GACCGA 1 cut(s) 239
TatI WGTACW 7 cut(s) 562, 1438, 1852, 2314, 2827, 4039, 4944
TauI GCSGC 3 cut(s) 103, 518, 1970
TseFI GTSAC 7 cut(s) 52, 676, 704, 1730, 1805, 2012, 6076
Tsp45I GTSAC 7 cut(s) 52, 676, 704, 1730, 1805, 2012, 6076
TspGWI ACGGA 1 cut(s) 5722
Van91I CCANNNNNTGG 2 cut(s) 3226, 4806
VneI GTGCAC 1 cut(s) 3093
VpaK11BI GGWCC 5 cut(s) 4302, 5104, 5511, 5924, 6192
VspI ATTAAT 3 cut(s) 2858, 5348, 5526
XagI CCTNNNNNAGG 1 cut(s) 5873
XapI RAATTY 7 cut(s) 139, 170, 329, 1521, 3277, 3919, 5423
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 6 cut(s) 350, 358, 1051, 1755, 3853, 5340
XcmI CCANNNNNNNNNTGG 1 cut(s) 1785
XhoI CTCGAG 1 cut(s) 2097
XmiI GTMKAC 2 cut(s) 186, 6114
XmnI GAANNNNTTC 5 cut(s) 1521, 3922, 4404, 4689, 5751
ZrmI AGTACT 4 cut(s) 564, 2316, 2829, 4946
Zsp2I ATGCAT 3 cut(s) 2353, 2461, 4891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.