RchiOBHm_Chr6g0296271

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009147
Physical Location & Seq
Reverse (-)
58004876 .. 58014854
9979 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26593

Sequence Viewer

Length: 6408 bp
ATGTCAAAGTCTTCCTCCCCTGAACTACGGGAACCCGTGTCTTCTTCTACTTCTCGGTCTAGGGATTTAGATGAAACAACAATGGATGATGAAGAAGGTACAATGGCAAGTGTTGCTGAATTTGTCGAGAAACTACATGCCAGCATGTCTTCACCGCATGAAAAAGAACTTATTACAGCACGTTTGCTAGGTATTGCCAAAGCAAGGAAGGATGCAAGAACAATTATTGGTTCACATTCCCAGGCCATGCCATTGTTCATAAATATTCTGAGGAGTGGCACCCCTGTTGCAAAGGTTAATGTTGCGGCAACTCTGAGCGTCCTGTGTAAAGATGAAGACTTGCGTCTTAAAGTACTTCTAGGTGGGTGCATCCCCCCGTTACTCTCACTTTTGAAGTCCGAATCAACTGAGGCCAGGAAGGCTGCAGCCGAAGCTATATATGAAGTTTCCTCTGGTGGGCTTTCAGATGATCACGTTGGCATAAAAATATTTATTACAGAAGGTGTTGTACCAAACTTGTGGAATCAGCTCAATCCAAACAGCAACCAGGACAAAGTGGTAGAGGGATTTGTGACAGGGGCTTTAAGAAATCTCTGTGGTGACAAGGATGGCTACTGGAGAGCAACACTTGAGGCTGGTGGAGTAGATATCACTGTGGGTCTCCTGTATTCTGACAATGCTGCTGCCCAGTCCAATGCTGCGTCCCTTTTGGCCCGTCTCATGTTGGCTTTCAGTGATAGCATCCCCAAAGTAATAGATTCTGGAGCTGTCAAAGCTTTGCTTCGGCTTGTGGGTCAAGAAAATAACGTTTCTGTTCGTGCTAGTGCTGCTGATGCTCTGGAAGCTCTTTCTTCAAAGTCAACTGGCGCTAAGAAAGCTATTGTTGATGCAAATGGTCTTCCAGTTCTGATTGGAGCTATTGTTGCTCCATCTAAGGAGTGCATGCAAGGTGAGTGTGGGCAGGCTCTCCAGGAGCATTCAGCAAGGGCTTTAGCAAATATTTGTGGTGGGGTGTCTGCTTTAATACTTTATCTTGGAGAGCTTTCACAGTCAGCTCGCCTAGCTGCCCCAGTTGCTGATATTATTGGAGCTCTTGCATACACTTTAATGGTCTTTGAGCAAAAATCTGGTGATGGTAAGGAATCTTTTAAGGTAACAAAGATAGAGGATATTCTTGTAATGCTGCTAAAGCCTCGTGATAATAAGCTTGTTCAAGAGCGTGTCCTTGAGGCCATGGCTAGTCTATATGGCAACATTCATCTCTCAAAATGGCTCAATCATGCAGAAGCGAAGAAGGTGCTTATTGGACTTATAACAATGGCTGCTGCTGATGTACAAGAGTCTCTGATACTCTCTTTGACAAGCTTATGCTGTGATGGTGTTGGAATCTGGGAGTCCATTGGCGAGAGAGAAGGAATTCAGCTACTTATTTCATTGCTTGGCTTATCCAGTGAGCAGCATCAAGAATATGCTGTTCAGTTGCTAGGGATCTTAACTGACCAGGTTGATGACAGCAAGTGGGCTATTACTGCTGCTGGTGGGATTCCTCCTCTAGTGCAGTTGTTAGAGACGGGATCGCAAAAGGCGAAGGAGGACGCTGCTCATGTGTTGTGGAACTTGTGCTGTCACAGTGAGGATATTCGTGCATGTGTTGAAAGTGCAGGAGCCATTCCAGCATTTTTATGGCTTCTAAAAAGTGGTGGATCAAGGGGGCAAGAAGCATCAGCTATGGCACTTACAAAGCTTATCCGAACAGCCGATTCGGCTACCATTAATCAGTTGTTAGCTTTGCTCTTAGGTGACTCTCCATGCTCAAAGGCCCATACTATCAGGGTGTTGGGTCATGTACTCACAATGGCATCACACAAGGATCTTGTGCATAAGGGTTCGGCAGCTAATAAAGGGTTGAGGTCTCTCGTTCAGGTTCTCAACTCATCAAATGAAGAAACTCAAGAGTATGCCGTGTCTGTCTTAGCTGATCTATTCAGCATGAGACAAGATATTTGTGACACTCTTGCAACTGATGAGATCGTGCATCCTTGCATGAAGCTTTTGACCAGCAGCACACAAGCTGTTGCAACACAGTCGGCTCGAGCTTTGGGTGCTCTTTCCCGTCCTATGAAGACCAAAACAATAAGCAAGATGTCTTATATTGCAGAAGGGGATGTTAAACCCCTCATCAAACTAGCAAAAACTTCTTCAATTGATGCTGCTCAAACTGCAGTTGCTGCACTCGCCAATCTTCTCTCTGATCCCCAGATTGCTGCAGAAGCCCTGGCAGAAGATGTTGTTTCAGCTTTGATAAGAGTACTGGGAGATGGAACTACAGAAGGTAAAAAGAATGCATCCCGTGCCCTTCATCAATTGTTGAAGCATTTTCCAGTAGGTGATGTGCTCACAGGAAATGCTCATTGCCGTTTTGCTATTCTTGCTGTTGTTGATTCCTTAAATGCATTGGATATGGATGAGACTGATGCTGCTGATGCTTTAGAAGTAGTAGCACTTTTGGCCAGAACCAAACAGGGTGTGAACTTCACTTACCCTCCATGGTCTGTATTTGCTGAAGTTGAATCGAGCTTAGAACCTCTTGTCCGCTGTCTGGCCGAAGGGTCTCCTTTGCTTCAGGACAAGGCAATAGAAGTTTTGTCTAGGCTTTGCGGGGAGCAACCAGTTGTGCTTGGTGATCTATTGATTGCAAGATCTGGATCCCTTGGTTCACTAGCGAACAGAATAATGAACTCATCTAGTTTAGAAGTCAGAGTTGGAGGAGCTGCATTACTCATTTGTGCAGCAAAAGAGCACAAAGAGCAGTGGATGGAAGTACTTGAGGTGTCAGGATATTTAAAACCATTAATGTGTGCTTTAGTGGATATGATGAAGCAAAATTGTAGCTGCTCCTCTTTAGAAATTGAAGTCCGAACTTCTAAAGCTTTTATGGAAAGAACTGCATTCCACGAAGGGGATGAGTTTGATGCCCCTGATCCAGCCATTGTCTTGGGGGGTACTGTTGCTTTGTGGTTGCTATGTATAATTGCTTCCTGTAATGCAAAGAGCAAACTCACCATTATGGAAGCCGGAGGTCTTGAGGCTCTCTGTGACAAGCTTGAGAGCCACACTTCCAATCCACAGGCAGATTATGAGGATACTGAAGGTATATGGATTAGTGCCCTGCTTCTGGCTATTCTCTTCCAAGATGCCAATGTCATTTCATCTCCTACAACAATGCGGATCATAGCTTCTCTTGCGCTTCTGCTGAGATCAGATGAGGTGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCAGTCTTGTTTGCAGTGGTAATAAGGGAATAATTCTTGCCATTGCAAATTCAGGTGCTGTTGCTGGATTGATAACCCTCATTGGTTACGTAGAATCTGATATGCCTAACCTTGTTACTTTATCAGAAGAGTTTTCTCTGGTGCGAAACCCTGATCAAGTTGTTTTGGAATACCTTTTTGATTTTGAAGATGTAAGAGTTGGATCCACAGCACGCAAATCTATACCTCTCTTGGTAGATCTCTTGAGACCAATGCCAGATAGACCTGGTGCCCCCCCAGTTGCTCTTAAACTCTTGACCTGTATTGCTGATGGAAGTGATACAAACAAATTAGTCATGGCTGAAGCTGGAGCTCTGGACGCTCTGACAAAGTACCTGTCTTTGAGCCCACAAGATTCGACTGAAGCTGCAATATCTGATTTATTCAGAATATTGTTTAGCCATCCTGATCTGATTCGCTATGAAGCATCAGCTAGTTCGTTGAATCAGCTCATAGCTGTTCTGCGTCTGGGGTCAAGAAATGCTAGATACAGTGCTGCAAGAGCTCTTCACGAACTATTTGATGCTGAGAACATCAGAGATTCTGATTTAGCCAGGCAATCTGTTCAGCCATTGGTTGACATGCTTAACGCCGCATCAGAGAATGAACAAGAGGCTGCTCTTGTTGCTGTAATTAAGCTGACTTCCGGAAATTCTTTTAGCGCAGCTTTGTTGACTGATGTGGAGGGAAATCCACTGGAGAGTTTATTTAAAATATTGTCTTCTGCTTCATCCTTGGATTTGAAGAGACTTGCTGCACAACTATGTTGTGTACTGTTTGATAGTACCAAAGTCAGAGGAAATCCAATTGCCTCCGAATGTATAGAGCCTCTTATTTCCTTGATGCTTTCTGGTATAAATGCAGCTGTAGAAGCTGGGGTTTGTGCCTTTGAAAAATTGTTGGATGATGAACACCAGGTAGAGCTTGCAGTGAACTATAATGTTGTCGATCTTCTTGTTGGATTAGTTTCTGGTACGAACAGTCAGCTCATTGAGGCTAGCATCTGTTCTCTCATAAAGTTGGGGAAAGACCGGACCCAATGCAAATCAGATATGATCAATGCTGGCATTATTGATAACTGTCTTGAGCTACTCCCTGTTGCAGCCAGTTCATTATGCTCTTCTATTGCTGAATTATTTCGCATTTTGACAAATAGTGATGCAATTGCTAGAAGTTTAGCTGCTGCAACAATTGTAGAACCCCTTTTCCTAGTTTTACTTCGTCCAGATTTCAGTATGTGGGGACAGCACAGTGCATTGCAAGCGCTAGTAAATATTTTGGAGAAGCCACAAAGCCTTGCCACTTTAAAACTTACGCCTAGCCAAGTCATTGAGCCTCTGATTTCATTTCTTGAATCCCCATCTCTAGCCATCCAGCAGCTTGGTACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCAAGATATTACAACAAAAAATGCTGTTGTTCCTCTTGTGCAACTTGCTGGAATTGGAATACTGAACTTACAGCAAACAGCGATAAAGGCATTGGAAAAAATATCCACAAGATGGCCAAAGGCAGTTGCAGATGCTGGAGGTATTTTTGAGCTTGGAAAGGTTATTATACAAGATGATCCTCAGCCACCTCATGCATTGTGGGAATCAGCTGCTTTAGTTCTCTCAAATGTTTTGTGCTTCAATGCTGAGTACTACTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACAGTGGATAGCACTATTACGGTGGCTCTCAATGCACTACTTGTTCATGAAAGGAGTGATAAGCTAAGTGCTGAACAGATGACTGAAAATGGTGCTATAGATGCCTTGTTGGACCTTTTAACATCTCATCAGTGTGAAGAAGCATCTGGAAGGTTACTTGAAGCGTTATTCAACAATGTCAGGATACGAGCAATGAAGGTTTCCAAGTATGCAATAGCACCTTTATCACAGTATCTGTTGGATTCACAAACCAAATCACAATCTGGAAAACTTCTCGTAGCTCTAGCTCTTGGAGACCTTTCCCAACACGAAGGGCTTGCTAGAGCCAGTGATTCTGTTTCTGCATGTCGGGCATTAATAAGTTTGCTTGAAGATCAGCCCACAGAAGATATGAAAATGGTGGCAACTTGTGCATTGCAAAATTTTGTCATGAACAGCCGAACAAATCGGCGTGCTGTCGCAGAAGCAGGGGGAATACTGGTTATTCAAGAACTACTACTGTCTCTCAATGCAGAAGTTGCTGGGCAAGCAGCGTTGCTTATCAAATTTTTGTTTTCTAATCACACACTTCAAGAATATGTGTCAAACGAGCTCATCAGATCTTTGACAGCCGCATTAGAGAGAGAGTTGTGGTCCACAGCAACTATTAATGAAGAGGTCTTGAGAGCCCTACATATGATATTTACCAACTTTCCTAAGCTCCATATTTCCGAGGCAGCCACTCTCTGCATTCCCAATCTGATTGGAGCCCTTAAATCTGGCAGTGAGGCAGCTCAGGATGTCGTGTTGGACACCCTTTGCTTGTTGAGACATTCTTGGTCAACCATGCCAATAGATATTGCAAAGTCTCAATCCGTGGTTGCTGCTGAAGCCATTCCCATCTTACAAAAGCTTATGAAAACCTGCCCACCAAGTTTCCATGAGAGAGCAGACAGCCTGTTGCATTGCTTACCAGGTTGTTTGACTGTTACAATCAAGCGTGGAAACAACCTTAAACAGGCTATGGGAGGCACAAATGCTTTTTGCCGATTGACAATAGGCAACGGTCCTCCTCGACAAACCAAGGTAGTGAGCCACAGTACTTCTCCCGAATGGAAAGAAGGGTTTACGTGGGCATTTGATGTGCCCCCAAAGGGACAAAAGCTCCACATCATCTGCAAAAGCAAAAATACCTTTGGAAAGTCAACACTGGGGAGAGTGACTATCCAGATCGATAAAGTTGTGTCAGAAGGAGTATACAGTGGATTATTCAGCCTAAACCATGACAACAACAAAGATGGATCTTCACGTACACTAGAAATCGAGATTATCTGGTCCAACAGAATGCCCGATGATGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2135

Amino Acids

229.05

Weight (kDa)

5.24

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 67 - 288 5.8e-06 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 380 - 571 2.7e-07 ARMC5
Arm PF00514 504 - 541 2.6e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 611 - 784 4.4e-08 ARMC5
C2 PF00168 2008 - 2106 5.7e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1313
Acc36I ACCTGC 1 cut(s) 5975
AccB1I GGYRCC 2 cut(s) 278, 3569
AccB7I CCANNNNNTGG 2 cut(s) 3298, 4878
AccI GTMKAC 1 cut(s) 6300
AccIII TCCGGA 1 cut(s) 3986
AciI CCGC 7 cut(s) 155, 305, 2593, 2658, 3226, 3933, 5676
AclI AACGTT 1 cut(s) 807
AcoI YGGCCR 4 cut(s) 2508, 2601, 3300, 4880
AcsI RAATTY 6 cut(s) 119, 1416, 3349, 3991, 5485, 5609
AcuI CTGAAG 6 cut(s) 2583, 2606, 3168, 3663, 3723, 5952
AfeI AGCGCT 1 cut(s) 4605
AfiI CCNNNNNNNGG 9 cut(s) 204, 456, 2599, 2959, 3175, 3298, 4878, 6061, 6197
AjuI GAANNNNNNNTTGG 2 cut(s) 2745, 2777
AloI GAACNNNNNNTCC 4 cut(s) 2574, 2606, 4530, 4562
Alw21I GWGCWC 7 cut(s) 1093, 2107, 2397, 2802, 3655, 3847, 5658
AlwNI CAGNNNCTG 5 cut(s) 1076, 2228, 3359, 4901, 5299
Ama87I CYCGRG 1 cut(s) 2091
Aor13HI TCCGGA 1 cut(s) 3986
Aor51HI AGCGCT 1 cut(s) 4605
AoxI GGCC 9 cut(s) 243, 411, 711, 1230, 1818, 2508, 2601, 3300, 4880
ApoI RAATTY 6 cut(s) 119, 1416, 3349, 3991, 5485, 5609
ArsI GACNNNNNNTTYG 4 cut(s) 1986, 2018, 3662, 3694
AseI ATTAAT 4 cut(s) 1773, 2852, 5420, 5712
Asp700I GAANNNNTTC 4 cut(s) 1416, 4476, 4761, 5937
AspLEI GCGC 4 cut(s) 869, 3247, 4004, 4606
AspS9I GGNCC 7 cut(s) 712, 1819, 4374, 5176, 5697, 6110, 6378
AsuHPI GGTGA 9 cut(s) 144, 611, 962, 1142, 1811, 2399, 2693, 3052, 3280
AsuNHI GCTAGC 1 cut(s) 4337
AvaI CYCGRG 1 cut(s) 2091
AvaII GGWCC 5 cut(s) 4374, 5176, 5697, 6110, 6378
BaeGI GKGCMC 4 cut(s) 2356, 3169, 3574, 6192
BalI TGGCCA 3 cut(s) 2510, 3302, 4882
BamHI GGATCC 2 cut(s) 2705, 3503
BanI GGYRCC 2 cut(s) 278, 3569
BanII GRGCYC 7 cut(s) 1093, 3655, 3689, 3847, 5658, 5734, 5815
BarI GAAGNNNNNNTAC 4 cut(s) 492, 524, 3019, 3051
BauI CACGAG 1 cut(s) 1194
BbsI GAAGAC 7 cut(s) 3, 33, 141, 342, 890, 2129, 4053
Bbv12I GWGCWC 7 cut(s) 1093, 2107, 2397, 2802, 3655, 3847, 5658
BbvCI CCTCAGC 1 cut(s) 4947
BceAI ACGGC 2 cut(s) 1946, 2400
BcgI CGANNNNNNTGC 6 cut(s) 1279, 1313, 2067, 2101, 5363, 5397
BciVI GTATCC 2 cut(s) 3136, 5241
BclI TGATCA 3 cut(s) 469, 3454, 4395
BfmI CTRYAG 6 cut(s) 423, 2220, 2265, 2325, 4206, 5160
BfoI RGCGCY 2 cut(s) 870, 4607
BfuAI ACCTGC 1 cut(s) 5975
BfuI GTATCC 2 cut(s) 3136, 5241
BglI GCCNNNNNGGC 2 cut(s) 419, 1763
BglII AGATCT 3 cut(s) 2699, 3538, 5663
BmcAI AGTACT 5 cut(s) 354, 2310, 2823, 5018, 6145
Bme18I GGWCC 5 cut(s) 4374, 5176, 5697, 6110, 6378
BmeT110I CYCGRG 1 cut(s) 2091
BmgT120I GGNCC 7 cut(s) 712, 1819, 4374, 5176, 5697, 6110, 6378
BmiI GGNNCC 9 cut(s) 33, 280, 1666, 2707, 3505, 3571, 4376, 5031, 5812
BmrI ACTGGG 6 cut(s) 682, 1064, 2321, 3286, 3572, 6263
BmtI GCTAGC 1 cut(s) 4341
BmuI ACTGGG 6 cut(s) 682, 1064, 2321, 3286, 3572, 6263
BoxI GACNNNNGTC 1 cut(s) 342
BpiI GAAGAC 7 cut(s) 3, 33, 141, 342, 890, 2129, 4053
BpmI CTGGAG 6 cut(s) 637, 783, 953, 3669, 4058, 4923
Bpu10I CCTNAGC 3 cut(s) 4947, 5760, 5838
BpuEI CTTGAG 9 cut(s) 650, 1247, 1935, 2846, 3104, 3125, 3565, 4445, 5746
Bsa29I ATCGAT 1 cut(s) 6276
BsaAI YACGTR 3 cut(s) 3391, 6174, 6353
BsaBI GATNNNNATC 1 cut(s) 2704
BsaI GGTCTC 5 cut(s) 665, 1917, 2616, 3541, 5352
BsaJI CCNNGG 9 cut(s) 240, 1233, 2274, 2546, 2710, 4074, 5775, 5919, 6126
BsaWI WCCGGW 2 cut(s) 3986, 4371
BsaXI ACNNNNNCTCC 6 cut(s) 906, 936, 2527, 2557, 6192, 6222
Bsc4I CCNNNNNNNGG 9 cut(s) 204, 456, 2599, 2959, 3175, 3298, 4878, 6061, 6197
Bse3DI GCAATG 7 cut(s) 1433, 2410, 3342, 4595, 5262, 5477, 6007
Bse8I GATNNNNATC 1 cut(s) 2704
BseAI TCCGGA 1 cut(s) 3986
BseCI ATCGAT 1 cut(s) 6276
BseDI CCNNGG 9 cut(s) 240, 1233, 2274, 2546, 2710, 4074, 5775, 5919, 6126
BseJI GATNNNNATC 1 cut(s) 2704
BseLI CCNNNNNNNGG 9 cut(s) 204, 456, 2599, 2959, 3175, 3298, 4878, 6061, 6197
BseMI GCAATG 7 cut(s) 1433, 2410, 3342, 4595, 5262, 5477, 6007
BseMII CTCAG 8 cut(s) 260, 305, 399, 3245, 3858, 4961, 5004, 5852
BseRI GAGGAG 5 cut(s) 286, 1539, 2781, 2887, 6105
BseSI GKGCMC 4 cut(s) 2356, 3169, 3574, 6192
BseYI CCCAGC 2 cut(s) 4214, 5585
BsgI GTGCAG 5 cut(s) 1577, 1680, 2214, 2808, 4080
BshFI GGCC 9 cut(s) 245, 413, 713, 1232, 1820, 2510, 2603, 3302, 4882
BshNI GGYRCC 2 cut(s) 278, 3569
BshVI ATCGAT 1 cut(s) 6276
BsiHKAI GWGCWC 7 cut(s) 1093, 2107, 2397, 2802, 3655, 3847, 5658
BsiHKCI CYCGRG 1 cut(s) 2091
BsiSI CCGG 3 cut(s) 3075, 3987, 4372
BslFI GGGAC 3 cut(s) 688, 4596, 6213
BslI CCNNNNNNNGG 9 cut(s) 204, 456, 2599, 2959, 3175, 3298, 4878, 6061, 6197
BsmBI CGTCTC 2 cut(s) 722, 1562
BsmFI GGGAC 3 cut(s) 688, 4596, 6213
BsmI GAATGC 6 cut(s) 976, 2347, 2948, 5059, 5793, 6393
BsnI GGCC 9 cut(s) 245, 413, 713, 1232, 1820, 2510, 2603, 3302, 4882
Bso31I GGTCTC 5 cut(s) 665, 1917, 2616, 3541, 5352
BsoBI CYCGRG 1 cut(s) 2091
Bsp13I TCCGGA 1 cut(s) 3986
Bsp1407I TGTACA 1 cut(s) 1333
Bsp19I CCATGG 2 cut(s) 1233, 2546
BspACI CCGC 7 cut(s) 155, 305, 2593, 2658, 3226, 3933, 5676
BspANI GGCC 9 cut(s) 245, 413, 713, 1232, 1820, 2510, 2603, 3302, 4882
BspCNI CTCAG 8 cut(s) 261, 306, 400, 3246, 3859, 4960, 5005, 5851
BspDI ATCGAT 1 cut(s) 6276
BspEI TCCGGA 1 cut(s) 3986
BspHI TCATGA 2 cut(s) 5110, 5493
BspLI GGNNCC 9 cut(s) 33, 280, 1666, 2707, 3505, 3571, 4376, 5031, 5812
BspMAI CTGCAG 3 cut(s) 427, 2224, 2269
BspMI ACCTGC 1 cut(s) 5975
BspOI GCTAGC 1 cut(s) 4341
BspQI GCTCTTC 2 cut(s) 3852, 4465
BspT107I GGYRCC 2 cut(s) 278, 3569
BspTNI GGTCTC 5 cut(s) 665, 1917, 2616, 3541, 5352
BsrDI GCAATG 7 cut(s) 1433, 2410, 3342, 4595, 5262, 5477, 6007
BsrGI TGTACA 1 cut(s) 1333
BssECI CCNNGG 9 cut(s) 240, 1233, 2274, 2546, 2710, 4074, 5775, 5919, 6126
BssNAI GTATAC 1 cut(s) 6301
BssSI CACGAG 1 cut(s) 1194
BssT1I CCWWGG 5 cut(s) 1233, 2546, 2710, 4074, 6126
Bst1107I GTATAC 1 cut(s) 6301
Bst2BI CACGAG 1 cut(s) 1194
Bst6I CTCTTC 6 cut(s) 3191, 3423, 3852, 4079, 4465, 5712
BstAPI GCANNNNNTGC 5 cut(s) 113, 2228, 2351, 5474, 5582
BstAUI TGTACA 1 cut(s) 1333
BstBAI YACGTR 3 cut(s) 3391, 6174, 6353
BstDSI CCRYGG 3 cut(s) 1233, 2546, 5919
BstENI CCTNNNNNAGG 1 cut(s) 6059
BstH2I RGCGCY 2 cut(s) 870, 4607
BstHHI GCGC 4 cut(s) 869, 3247, 4004, 4606
BstNSI RCATGY 6 cut(s) 140, 148, 946, 1650, 3925, 5412
BstPAI GACNNNNGTC 1 cut(s) 342
BstSFI CTRYAG 6 cut(s) 423, 2220, 2265, 2325, 4206, 5160
BstSLI GKGCMC 4 cut(s) 2356, 3169, 3574, 6192
BstSNI TACGTA 1 cut(s) 3391
BstV2I GAAGAC 7 cut(s) 3, 33, 141, 342, 890, 2129, 4053
BstX2I RGATCY 8 cut(s) 1488, 1870, 2699, 2705, 3503, 3538, 5663, 6344
BstXI CCANNNNNNTGG 3 cut(s) 519, 2995, 4721
BstYI RGATCY 8 cut(s) 1488, 1870, 2699, 2705, 3503, 3538, 5663, 6344
BstZ17I GTATAC 1 cut(s) 6301
Bsu15I ATCGAT 1 cut(s) 6276
BsuI GTATCC 2 cut(s) 3136, 5241
BsuRI GGCC 9 cut(s) 245, 413, 713, 1232, 1820, 2510, 2603, 3302, 4882
BsuTUI ATCGAT 1 cut(s) 6276
BtgI CCRYGG 3 cut(s) 1233, 2546, 5919
BtsI GCAGTG 4 cut(s) 2816, 3322, 4275, 5833
BveI ACCTGC 1 cut(s) 5975
CaiI CAGNNNCTG 5 cut(s) 1076, 2228, 3359, 4901, 5299
CciI TCATGA 2 cut(s) 5110, 5493
CfoI GCGC 4 cut(s) 869, 3247, 4004, 4606
Cfr13I GGNCC 7 cut(s) 712, 1819, 4374, 5176, 5697, 6110, 6378
ClaI ATCGAT 1 cut(s) 6276
CseI GACGC 6 cut(s) 307, 332, 690, 1604, 3668, 3794
CsiI ACCWGGT 4 cut(s) 1500, 3565, 4254, 6016
DraI TTTAAA 3 cut(s) 2844, 4051, 4647
EaeI YGGCCR 4 cut(s) 2508, 2601, 3300, 4880
Eam1104I CTCTTC 6 cut(s) 3191, 3423, 3852, 4079, 4465, 5712
EarI CTCTTC 6 cut(s) 3191, 3423, 3852, 4079, 4465, 5712
Ecl136II GAGCTC 4 cut(s) 1091, 3653, 3845, 5656
Eco105I TACGTA 1 cut(s) 3391
Eco130I CCWWGG 5 cut(s) 1233, 2546, 2710, 4074, 6126
Eco24I GRGCYC 7 cut(s) 1093, 3655, 3689, 3847, 5658, 5734, 5815
Eco31I GGTCTC 5 cut(s) 665, 1917, 2616, 3541, 5352
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 5 cut(s) 4374, 5176, 5697, 6110, 6378
Eco47III AGCGCT 1 cut(s) 4605
Eco53kI GAGCTC 4 cut(s) 1091, 3653, 3845, 5656
Eco57I CTGAAG 6 cut(s) 2583, 2606, 3168, 3663, 3723, 5952
Eco88I CYCGRG 1 cut(s) 2091
EcoICRI GAGCTC 4 cut(s) 1091, 3653, 3845, 5656
EcoNI CCTNNNNNAGG 1 cut(s) 6059
EcoRI GAATTC 1 cut(s) 1416
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 5 cut(s) 1233, 2546, 2710, 4074, 6126
EcoT22I ATGCAT 3 cut(s) 2347, 2455, 4963
EcoT38I GRGCYC 7 cut(s) 1093, 3655, 3689, 3847, 5658, 5734, 5815
ErhI CCWWGG 5 cut(s) 1233, 2546, 2710, 4074, 6126
Esp3I CGTCTC 2 cut(s) 722, 1562
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FaqI GGGAC 3 cut(s) 688, 4596, 6213
FauI CCCGC 1 cut(s) 2651
FauNDI CATATG 1 cut(s) 5739
FbaI TGATCA 3 cut(s) 469, 3454, 4395
FblI GTMKAC 1 cut(s) 6300
FriOI GRGCYC 7 cut(s) 1093, 3655, 3689, 3847, 5658, 5734, 5815
GlaI GCGC 4 cut(s) 868, 3246, 4003, 4605
GsaI CCCAGC 2 cut(s) 4218, 5589
GsuI CTGGAG 6 cut(s) 637, 783, 953, 3669, 4058, 4923
HaeII RGCGCY 2 cut(s) 870, 4607
HaeIII GGCC 9 cut(s) 245, 413, 713, 1232, 1820, 2510, 2603, 3302, 4882
HapII CCGG 3 cut(s) 3075, 3987, 4372
HgaI GACGC 6 cut(s) 307, 332, 690, 1604, 3668, 3794
HhaI GCGC 4 cut(s) 869, 3247, 4004, 4606
Hin6I GCGC 4 cut(s) 867, 3245, 4002, 4604
HinP1I GCGC 4 cut(s) 867, 3245, 4002, 4604
HincII GTYRAC 5 cut(s) 861, 3919, 4014, 5886, 6249
HindII GTYRAC 5 cut(s) 861, 3919, 4014, 5886, 6249
HindIII AAGCTT 8 cut(s) 774, 1205, 1363, 1742, 2048, 2928, 3101, 5954
HpaII CCGG 3 cut(s) 3075, 3987, 4372
HphI GGTGA 9 cut(s) 144, 611, 962, 1142, 1811, 2399, 2693, 3052, 3280
HpyCH4IV ACGT 6 cut(s) 181, 474, 807, 3390, 6173, 6352
HpySE526I ACGT 6 cut(s) 181, 474, 807, 3390, 6173, 6352
HspAI GCGC 4 cut(s) 867, 3245, 4002, 4604
Kpn2I TCCGGA 1 cut(s) 3986
Ksp22I TGATCA 3 cut(s) 469, 3454, 4395
LguI GCTCTTC 2 cut(s) 3852, 4465
MabI ACCWGGT 4 cut(s) 1500, 3565, 4254, 6016
MaeII ACGT 6 cut(s) 181, 474, 807, 3390, 6173, 6352
MfeI CAATTG 5 cut(s) 2202, 2363, 4146, 4503, 4530
MflI RGATCY 8 cut(s) 1488, 1870, 2699, 2705, 3503, 3538, 5663, 6344
MlsI TGGCCA 3 cut(s) 2510, 3302, 4882
MluNI TGGCCA 3 cut(s) 2510, 3302, 4882
MlyI GAGTC 3 cut(s) 1349, 1403, 1796
MmeI TCCRAC 9 cut(s) 1363, 2743, 3481, 4221, 4279, 5154, 5283, 5832, 6405
Mox20I TGGCCA 3 cut(s) 2510, 3302, 4882
Mph1103I ATGCAT 3 cut(s) 2347, 2455, 4963
MroI TCCGGA 1 cut(s) 3986
MroXI GAANNNNTTC 4 cut(s) 1416, 4476, 4761, 5937
MscI TGGCCA 3 cut(s) 2510, 3302, 4882
MslI CAYNNNNRTG 6 cut(s) 1106, 1681, 2854, 3065, 4102, 5196
Msp20I TGGCCA 3 cut(s) 2510, 3302, 4882
MspA1I CMGCKG 3 cut(s) 2595, 4205, 4976
MspI CCGG 3 cut(s) 3075, 3987, 4372
MunI CAATTG 5 cut(s) 2202, 2363, 4146, 4503, 4530
Mva1269I GAATGC 6 cut(s) 976, 2347, 2948, 5059, 5793, 6393
NcoI CCATGG 2 cut(s) 1233, 2546
NdeI CATATG 1 cut(s) 5739
NheI GCTAGC 1 cut(s) 4337
NlaIV GGNNCC 9 cut(s) 33, 280, 1666, 2707, 3505, 3571, 4376, 5031, 5812
NmuCI GTSAC 7 cut(s) 571, 599, 1625, 1799, 2006, 3095, 6262
NsiI ATGCAT 3 cut(s) 2347, 2455, 4963
NspI RCATGY 6 cut(s) 140, 148, 946, 1650, 3925, 5412
PaeI GCATGC 1 cut(s) 946
PaeR7I CTCGAG 1 cut(s) 2091
PagI TCATGA 2 cut(s) 5110, 5493
PciSI GCTCTTC 2 cut(s) 3852, 4465
PcsI WCGNNNNNNNCGW 1 cut(s) 1583
PctI GAATGC 6 cut(s) 976, 2347, 2948, 5059, 5793, 6393
PdmI GAANNNNTTC 4 cut(s) 1416, 4476, 4761, 5937
PflMI CCANNNNNTGG 2 cut(s) 3298, 4878
PfoI TCCNGGA 1 cut(s) 969
PleI GAGTC 3 cut(s) 1348, 1402, 1796
PpsI GAGTC 3 cut(s) 1348, 1402, 1796
Ppu21I YACGTR 3 cut(s) 3391, 6174, 6353
PshAI GACNNNNGTC 1 cut(s) 342
PshBI ATTAAT 4 cut(s) 1773, 2852, 5420, 5712
PsiI TTATAA 1 cut(s) 1313
Psp124BI GAGCTC 4 cut(s) 1093, 3655, 3847, 5658
Psp1406I AACGTT 1 cut(s) 807
PspFI CCCAGC 2 cut(s) 4214, 5585
PspN4I GGNNCC 9 cut(s) 33, 280, 1666, 2707, 3505, 3571, 4376, 5031, 5812
PspPI GGNCC 7 cut(s) 712, 1819, 4374, 5176, 5697, 6110, 6378
PspXI VCTCGAGB 1 cut(s) 2091
PsrI GAACNNNNNNTAC 2 cut(s) 2522, 2554
PstI CTGCAG 3 cut(s) 427, 2224, 2269
PstNI CAGNNNCTG 5 cut(s) 1076, 2228, 3359, 4901, 5299
PsuI RGATCY 8 cut(s) 1488, 1870, 2699, 2705, 3503, 3538, 5663, 6344
PvuII CAGCTG 2 cut(s) 4205, 4976
RseI CAYNNNNRTG 6 cut(s) 1106, 1681, 2854, 3065, 4102, 5196
SacI GAGCTC 4 cut(s) 1093, 3655, 3847, 5658
SapI GCTCTTC 2 cut(s) 3852, 4465
Sau96I GGNCC 7 cut(s) 712, 1819, 4374, 5176, 5697, 6110, 6378
ScaI AGTACT 5 cut(s) 354, 2310, 2823, 5018, 6145
SchI GAGTC 3 cut(s) 1349, 1403, 1796
SexAI ACCWGGT 4 cut(s) 1500, 3565, 4254, 6016
SfcI CTRYAG 6 cut(s) 423, 2220, 2265, 2325, 4206, 5160
Sfr274I CTCGAG 1 cut(s) 2091
SinI GGWCC 5 cut(s) 4374, 5176, 5697, 6110, 6378
SlaI CTCGAG 1 cut(s) 2091
SmiMI CAYNNNNRTG 6 cut(s) 1106, 1681, 2854, 3065, 4102, 5196
SnaBI TACGTA 1 cut(s) 3391
SphI GCATGC 1 cut(s) 946
SsiI CCGC 7 cut(s) 155, 305, 2593, 2658, 3226, 3933, 5676
SspI AATATT 6 cut(s) 265, 489, 1000, 3732, 4056, 4615
SstI GAGCTC 4 cut(s) 1093, 3655, 3847, 5658
StyI CCWWGG 5 cut(s) 1233, 2546, 2710, 4074, 6126
TaiI ACGT 6 cut(s) 184, 477, 810, 3393, 6176, 6355
TaqI TCGA 8 cut(s) 126, 2092, 2573, 3698, 4287, 6118, 6276, 6366
TaqII GACCGA 1 cut(s) 45
TatI WGTACW 8 cut(s) 352, 1333, 1846, 2308, 2821, 4111, 5016, 6143
TauI GCSGC 3 cut(s) 308, 3935, 5678
TseFI GTSAC 7 cut(s) 571, 599, 1625, 1799, 2006, 3095, 6262
Tsp45I GTSAC 7 cut(s) 571, 599, 1625, 1799, 2006, 3095, 6262
TspGWI ACGGA 1 cut(s) 5908
Van91I CCANNNNNTGG 2 cut(s) 3298, 4878
VpaK11BI GGWCC 5 cut(s) 4374, 5176, 5697, 6110, 6378
VspI ATTAAT 4 cut(s) 1773, 2852, 5420, 5712
XagI CCTNNNNNAGG 1 cut(s) 6059
XapI RAATTY 6 cut(s) 119, 1416, 3349, 3991, 5485, 5609
XceI RCATGY 6 cut(s) 140, 148, 946, 1650, 3925, 5412
XcmI CCANNNNNNNNNTGG 1 cut(s) 1680
XhoI CTCGAG 1 cut(s) 2091
XmiI GTMKAC 1 cut(s) 6300
XmnI GAANNNNTTC 4 cut(s) 1416, 4476, 4761, 5937
ZrmI AGTACT 5 cut(s) 354, 2310, 2823, 5018, 6145
Zsp2I ATGCAT 3 cut(s) 2347, 2455, 4963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.