MD15G1345100.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009147
Physical Location & Seq
Forward (+)
40454241 .. 40463783
9543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1345100.v1.1.491

Sequence Viewer

Length: 6405 bp
ATGTCAAAGTCTCCCTCCCCTAAACTGCGACCCATATCTTCTTCTACTTCACAGTCTAGGGTTTTAAAGGAACCAGCAATGGATGATGAAGAAGGTACAATGGCAAGAGTTGCTCAATTTGTCGAGCAACTACATGCCAGCATGTCTACACCGAAAGAAAAAGAACTTATTACAGCTCGATTGCTTGGTATTTCCAAAGCAAGAAAGGATGCAAGAGCAATTATTGGTTCGCATTCCCAGGCAATGCCATTGTTCATAAGCATTCTCAGGAATGGCACCCCTGCGGCAAAAGTTAATGTGGCTGCAACTTTGAGTGTCCTGTGTAAAGATGAAGACTTGCGTCTTAAAGTACTTCTAGGCGGATGCATCCCCGCTTTACTCTCACTTTTGAAGTCTGAATCAATTGAGGCTAGGAAGGCAGCAGCAGAAGCTATATACGAAGTTTCCTCTGGTGGGCTTTCAGATGATCATGTAGGCATAAAAATATTTATTACAGAAGGTGTAGTACCAAACTTGTGGAATCAACTCAATCCAAAGAGCAAGCAGGACAAAGTGGTTGAAGGTTTTGTGACAGGGGCTTTGAGAAATCTTTGTGGTGACAAGGATGGTTACTGGAGAGCAACACTTGAGGCTGGTGGAGTAGATATCATTGTGGGTCTTCTGTCTTCTGACAATGCTGCTGCCCAGTCCAATGCTGCCTCCCTTTTGGCCCGTCTTATGTTGGCTTTCAGTGATAGCATCCCCAAAGTAATAGATTCTGGAGCTGTCAAAGCTTTGCTTTGGCTTGTGGGTCAGGAAAATGATGTTTCTGTTCGTGCTAGTGCTGCTGATGCTCTGGAAGCCCTTTCTTCAAAGTCAACTGGGGCTAAAAAGGCTATTGTGAATGCAGATGGTCTTCGAGTTCTAATTGGGGCTATAGTTGCTCCATCTAAAGAATGCATGCAAGGTGAGTGTGGGCAGGCTCTTCAAGACCATGCCACACGGGCTTTAGCAAATATTTGTGGCGGGATGTCTGCTTTAATTTTGTATCTTGGAGAACTTTCACAGTCACCTCGCCTAGCTGCCCCGGTTGCTGATATTATTGGAGCTCTTGCATACACACTGATGGTCTTTGAGCACAATTCTGGTGCGGATCAGGATTCTGTTAATGTAACAAAGATAGAGGATATTCTTGTAATGCTGCTAAAGCCTCGGGACAATAAGCTTGTTCAAGAGCGTGTCCTTGAGGCCATGGCTAGTCTATATGGCAACAACTATCTCTCCAGTTGGCTCAATCATGCACAGGCAAAGAAGGTGCTCATTGGACTTATAACAATGGCTGCTGTTGACGTGCAAGAGTATCTTATACCCTCTTTGACAAGCTTATGCTGTGATGGTGTTGGAATATGGGAGTCCATTGGCAAGAGAGAAGGAATTCAGTTACTTATTTCACTGTTGGGGTTATCCAGTGAGCAGCATCAAGAATATGCTGTTCAGTTGCTGGCCATCTTAACTGACCAAGTTGATGACAGCAAGTGGGCTATTACTGCTGCCGGTGGGATTCCTCCTCTGGTACAGTTATTAGAGACGGGTTCTCAGAAGGCGAAAGAGGATGCCGCTCATGTGTTGTGGAATTTGTGCTGTCACAGTGAAGATATCCGTGCATGTGTTGAAAGTGCAGGAGCCATCCCAGCATTTTTATGGCTTCTGAAAAGTGGTGGGTCAAGAGGACAAGAAGCATCAGCCAAGGCACTCACAAAGCTTGTCCGGACAGCTGATTCTGCTACCATTAATCAGTTGTTAGTTTTGCTCCTCGGTGACTCTCCAAGCTCAAAGGCCCACACTATCAGGGTATTGGGTCATGCACTCATAATGGCCTCACACAAGGATCTTGTGCATAAAGGTTCAGCAGCTAATAAAGGGCTGAGGTCTCTTGTCCAGGTTCTCAATTCATCAAATGAAGAAACTCAAGAGTATGCTGCTTCTGTCCTAGCTGATCTATTCAGCACAAGACAAGATATTTGTGATACTCTTGCTACTGATGAGATCGTGCATCCTTGCATGAAGCTATTGACCAGCAACACACAAGGTGTTGCAACACAATCAGCTCGAGCATTGGGTGCTCTGTCCCGTCCTATGAAGACCAAACCAAGAAGCAAGATGTCTTATATTGCAGAAGGTGATGTCAAGCCCCTTATCAGACTTGCTAAAACTTCTTCCATTGATGCTGTTGAAACTGCAGTTGCCGCACTTGCCAATCTTCTCTCTGATCCCCAGATTGCTGCAGAAGCCCTGGCAGAAGATGTTGTTTCAGCTTTGATAAGAGTACTGGGTGATGGAACTTCAGAAGGTAAGAAGAATGCATCCCGTGCGCTTCATCAATTGCTGAAGCATTTTCCAATAGGTGATTTGCTCACAGGAAATGCTCAGTGTCGTTTTGCTATGCTTGCTATTGTTGATTCCTTAAATGCATTGGATATGGATGGGACTGATGCTGCGGATGCTTTAGAAGTAGTTTCCCTTTTGGCTAGAACAAAACAGGGCGTGAACTTCACTTACCCTCCATGGTCTGCCCTTGCTGAAGTTCCATCAAGCTTAGAACCTCTTGTTCGCTGCCTGGCTGAAGGGCCTCCCCCGCTTCAGGATAAGGCAATAGAAATTTTATCTAGGCTTTGCGGTGAGCAGCCAGTTGTGCTTGGTGATCTATTGATTGAAAGATCAAGCTCTCTTGGTTCACTAGCTAACAGGGTAATGAACTCATCTAGTCTAGAAATCAGAGTTGGAGGAGCTGCATTACTTATTTGTGCAGCAAAAGAGCACAAACAGAAGGCACTCGAAGTACTTGATGTGTCAGGATATTTGGAACCATTAACGTATGCTTTAGTGGACATGGTGAAGCGAAAGTCTAGCTGCTCCTTTCCAGAAATTGAAGTCAGAACTCCTAGAGGTTTTATTGAAAGAACTGCATTCCATGAAGGTGATGAGTTTGATGTCCCTGATCCAGCCATTGTTTTGGGGGGTACTGTTGCCTTGTGGTTGCTATGCATAATTGGTTCCTTTCATGCCAAGAGCAAACTCACCATTATGGAAGCTGGTGGTCTTGAGGTTCTATCTGAAAAGCTCGCAGGTTACACTTCCAATCCACAGGCAGAATATGAGGATACAGAAGGTATATGGATTAGTGCCCTGGTCCTGGCTATTCTGTTTGAAGATGCAAATGTTGTTCTGTCACCTGTAACAATGCGCATCATTCCTTCACTTGCTCTTCTTTTGAAATCGGATGAGATGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCAGTCTTGTTTCTAATGGTAGTAAAGGAATAATTCTTGCCCTTGCAAATTCGGGTGCTGTTGTTGGATTGATAACTCTTATTGGTTATGTAGAGTCCGACGTGCCTAACCTTGTTACTTTATCAGAAGAGTTTTCTCTGGTGCGAAATCCTGATCAAGTTGTTTTGGAGTGCCTTTTTGATTTTGAAGACATAAGAGTTGGATCAACAGCACGTAAATCCATACCTCTCTTGGTGGATCTCTTGAGACCAATGCCAGAACGGCCCGGTGCCCCTCCAATTGCTGTTAAACTCTTGACTCGTATCGCCAATGGAAGCGATACAAATAAGTTAATTGTGGGTGAAGCTGGAGCTCTGGATGCTCTGACAAAGTACCTGTCTCTGAGTCCACAAGACTCCACCGAAGCCACAATAACTGAATTATTCAGAATTTTATTTAGCAATCCTGATCTCATTCGATATGAAGCATCAGCTAGTTCGTTGAATCAACTCATAGCTGTTCTGCGACTGGGTTCAAGAAATGCAAGATATAGTGCTGCCAGAGCTCTTCATGAACTATTTGGTGCTGAGAACATTAGAGATTCTGATTTAGCCAGGCACTCAATTCAGCCATTGGTTGACATGCTTAATGCTGCATCAGAGAGTGAACAAGAGGCTGCCCTCATTGCATTAATAAAGTTGACTTCAGGAAATTCTTCTAAAGCAGCTTTGTTGACTGATGTGGGGGGAAACCCAATGGAGAGTTTGTACAAAATACTGTCTTCTGCTTCATCCTTGGAATTAAAGAGAACTGCTGCACAACTCTGTTGTACTTTGTTTGTTAATACTGAAGTCAGAGGAAATCCAATTGCCTCAGAATGCATAGAGCCCCTTATATCACTGATGCATGCTGATGCTACTACAGCTGTAGAAGCTGGAGTTTGTGCTTTTGAAAAATTGTTGGATGATGAACACCGGGTGGAGCTTGCAATGGCCTATAATGTTGTGGATCTCCTTGTTGGATTAGTTTCTGGAACAAGCATGCAGCTCATTGAGGCCAGTGTCTGTTCTCTCATAAAGTTAGGGAAAGACCGGACCCCATGCAAATTGGATATGGTAAATGCTGGAATCATTGATAAATGTCTTGAACTACTACCTGTTGCACCCAGTTCATTATGCTCTTCCATAGCTGAATTGTTCCGCATTTTAACAAATAGTAATGCAATTGCTAGAAGTTTGGCTGCTGCACAAATTGTAGAACCTCTTTTTATAGTTTTACAACGTCCAGATTTCAATTTATGGGGACAGCACAGTGCATTACAAGCACTAGTAAATATTTTGGAGAAGCCACAGAGCCTTGCAACGTTAAAGCTTACACCTAGCCAAGTGATCGAGCCTCTTATTTCATTTCTGGAATCCCCATCTCAAGCCATTCAGCAGCTTGGCACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCTAGATATTACAACAAAAAATGCAGTTGTTCCACTCGTTCAGCTTGCTGGAATTGGAATATTAAACTTACAGCAGACAGCGGTAAAGGCATTGGAAATAATCTCCACAAGCTGGCCAAAGGCAGTTGCCGATGCTGGTGGTATATTTGAGCTTGGAAAGGTTATTATTCAAGATGACCCTCAGCCACCTCATGCCCTGTGGGAATCAGCTGCTTTAGTTCTCTCAAATGTTCTGCACTTCAATGCTGAATACTATTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACAGTGGACAGCACCATCACGGTGGCCCTCAATGCGTTGCTTGTTCATGAAAGGAGTGATAATTTAAGTGCTGAACAGATGACTGATGGTGGTGCTATAGAAGCCTTGTTGGACCTTTTACGGTCTCATCAATGCGAAGAAGCATCTGGAAGATTACTTGAAGCTCTATTTAACAATGTCAGGATACGACAAATGAAGGTCTCTAAGTATGCAATAGCACCACTATCACAGTATCTGTTGGATCCACAAACCAAATCAGAGTCTGGAAAACTTCTTGCTGCTCTTGCTCTTGGAGACCTTTCCCAACATGAAGGGCTTGCAAGAGCCAGAGATTCCATTTCTGCATGTCGTGCATTGGTAAGCTTGCTTGAAGAACAGCCAACAGAAGAGATGAAAATGGTGTCAATTTGTGCATTGCAAAACTTCGTCGTGAACAGCCGAACGAATAGGCGAGCTGTCGCAGAAGCAGGGGGCATACTGATTATTCAAGAACTACTACTGTCTCCCAATGCAGAAACTGCTGGGCAGGCAGCATTGCTTATCAAATTTTTGTTTTCTAATCACACACTCCAAGAGTATGTATCAAATGAACTTGTCAGATCTTTGACAGCTGCATTGGAGAGAGAGTTGTGGTCCTCAGCAACTATTAATGAAGAGGTCTTGAGAGCCTTACATATGATATTCATCAACTTCCCAAAGCTCCATATTTCTGAAGCAGCCACTCTCTGCATTCCCAATTTGATCGGGGCTCTTAAATCTGGTAGCGACACGGCTCAGGATGTTGTTTTGGACACCCTTTCCCTGTTGAGACATTCTTGGTCAACCATGCCAATAGATATTGCAAAGTCTCAGGCTGTGATTGCTGCTGAAGCCATTCCCATCCTACAAATGCTCATGAGAACCTGTCCTCCAAGTTTCCATGAGAGAGCAGACAGCCTGTTGCATTGCTTACCAGGTTGTTTGACGGTTACAATTAAGCGGGGCAACAACCTTAAACAGGCCATGGGAGGCACAAATGCTTTTTGTCGATTGACAATAGGCAATGGCCCTCCTCGACAAACCAAGGTAGTGAGCCACAGTACCTCTCCTGAGTGGAAAGAAGGCTTTACGTGGGCATTTGATGTGCCTCCAAAGGGACAGAAACTCCACATCATTTGCAAAAGCAAAAATACCTTCGGAAAGACAACTCTGGGAAGAGTGACGATCCAAATTGACAAAGTCGTTAGTGAAGGAGTATACAGTGGATTGTTTAGTCTAAATCATGACAGCAACAAAGATGGGTCTTCCCGTACACTAGAAATCGAGATTATCTGGTCAAACAGAATGGCTGATGAAGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2135

Amino Acids

229.12

Weight (kDa)

5.48

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 67 - 287 1.7e-06 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 381 - 571 2.2e-07 ARMC5
Arm PF00514 503 - 540 2.6e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 633 - 783 6.7e-07 ARMC5
ARM_ARMC5 PF24768 1260 - 1400 1e-05 ARMC5
C2 PF00168 2007 - 2105 7e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1310
Acc16I TGCGCA 1 cut(s) 3224
Acc36I ACCTGC 1 cut(s) 3095
AccB1I GGYRCC 2 cut(s) 275, 3566
AccB7I CCANNNNNTGG 2 cut(s) 3295, 4875
AccBSI CCGCTC 1 cut(s) 1598
AccI GTMKAC 2 cut(s) 146, 6297
AccIII TCCGGA 1 cut(s) 1746
AclI AACGTT 1 cut(s) 4640
AcoI YGGCCR 3 cut(s) 1482, 3297, 4877
AcsI RAATTY 7 cut(s) 1413, 1612, 2637, 3346, 3726, 3988, 5606
AcuI CTGAAG 9 cut(s) 2307, 2387, 2580, 2603, 2622, 3966, 4146, 5793, 5949
AdeI CACNNNGTG 2 cut(s) 2069, 4255
AfiI CCNNNNNNNGG 7 cut(s) 453, 2620, 3172, 3295, 4875, 6058, 6194
AhlI ACTAGT 1 cut(s) 4603
AjiI CACGTC 2 cut(s) 1330, 3400
AjnI CCWGG 8 cut(s) 237, 1917, 2271, 2595, 3165, 3171, 3890, 6013
AjuI GAANNNNNNNTTGG 2 cut(s) 2742, 2774
AloI GAACNNNNNNTCC 2 cut(s) 5953, 5985
Alw21I GWGCWC 7 cut(s) 1090, 1119, 1299, 2104, 2799, 3652, 3844
AlwNI CAGNNNCTG 5 cut(s) 1480, 4341, 5296, 5324, 5579
Ama87I CYCGRG 2 cut(s) 1191, 2088
Aor13HI TCCGGA 1 cut(s) 1746
ApoI RAATTY 7 cut(s) 1413, 1612, 2637, 3346, 3726, 3988, 5606
ArsI GACNNNNNNTTYG 6 cut(s) 562, 594, 1983, 2015, 3659, 3691
AseI ATTAAT 3 cut(s) 1770, 3968, 5709
Asp700I GAANNNNTTC 4 cut(s) 1413, 3991, 4758, 5934
AspLEI GCGC 2 cut(s) 2353, 3225
AsuC2I CCSGG 3 cut(s) 1067, 3564, 4253
AvaI CYCGRG 2 cut(s) 1191, 2088
AvaII GGWCC 4 cut(s) 3169, 4371, 5173, 5694
BaeGI GKGCMC 2 cut(s) 3166, 3571
BalI TGGCCA 3 cut(s) 1484, 3299, 4879
BamHI GGATCC 1 cut(s) 5302
BanI GGYRCC 2 cut(s) 275, 3566
BanII GRGCYC 5 cut(s) 1090, 3652, 3844, 4167, 5812
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbsI GAAGAC 8 cut(s) 339, 650, 657, 887, 2126, 3492, 4050, 6336
Bbv12I GWGCWC 7 cut(s) 1090, 1119, 1299, 2104, 2799, 3652, 3844
BbvCI CCTCAGC 3 cut(s) 1904, 4944, 5698
BceAI ACGGC 2 cut(s) 3575, 5847
BciT130I CCWGG 8 cut(s) 239, 1919, 2273, 2597, 3167, 3173, 3892, 6015
BciVI GTATCC 2 cut(s) 3133, 5238
BclI TGATCA 2 cut(s) 466, 3451
BcnI CCSGG 3 cut(s) 1067, 3564, 4253
BcuI ACTAGT 1 cut(s) 4603
BfmI CTRYAG 6 cut(s) 915, 2217, 2262, 4197, 4203, 5157
BfuAI ACCTGC 1 cut(s) 3095
BfuI GTATCC 2 cut(s) 3133, 5238
BglI GCCNNNNNGGC 2 cut(s) 983, 3559
BglII AGATCT 1 cut(s) 5660
BmcAI AGTACT 3 cut(s) 351, 2307, 2820
Bme18I GGWCC 4 cut(s) 3169, 4371, 5173, 5694
BmeT110I CYCGRG 2 cut(s) 1191, 2088
BmgBI CACGTC 2 cut(s) 1330, 3400
BmiI GGNNCC 9 cut(s) 72, 277, 1663, 2844, 3034, 3568, 4373, 5028, 5304
BmrI ACTGGG 6 cut(s) 679, 870, 2318, 3283, 3815, 4437
BmuI ACTGGG 6 cut(s) 679, 870, 2318, 3283, 3815, 4437
BoxI GACNNNNGTC 1 cut(s) 339
BpiI GAAGAC 8 cut(s) 339, 650, 657, 887, 2126, 3492, 4050, 6336
BpmI CTGGAG 5 cut(s) 634, 780, 1246, 3666, 4233
Bpu10I CCTNAGC 4 cut(s) 1904, 4944, 5698, 5835
BpuEI CTTGAG 7 cut(s) 647, 1244, 1932, 3101, 3562, 4686, 5743
BpuMI CCSGG 3 cut(s) 1067, 3564, 4253
BsaAI YACGTR 2 cut(s) 3512, 6171
BsaBI GATNNNNATC 1 cut(s) 5744
BsaI GGTCTC 5 cut(s) 1914, 3538, 5190, 5266, 5349
BsaWI WCCGGW 2 cut(s) 1746, 4368
Bsc4I CCNNNNNNNGG 7 cut(s) 453, 2620, 3172, 3295, 4875, 6058, 6194
Bse118I RCCGGY 1 cut(s) 1532
Bse3DI GCAATG 8 cut(s) 84, 249, 3960, 4272, 5474, 5594, 6004, 6109
Bse8I GATNNNNATC 1 cut(s) 5744
BseAI TCCGGA 1 cut(s) 1746
BseBI CCWGG 8 cut(s) 239, 1919, 2273, 2597, 3167, 3173, 3892, 6015
BseJI GATNNNNATC 1 cut(s) 5744
BseLI CCNNNNNNNGG 7 cut(s) 453, 2620, 3172, 3295, 4875, 6058, 6194
BseMI GCAATG 8 cut(s) 84, 249, 3960, 4272, 5474, 5594, 6004, 6109
BseRI GAGGAG 4 cut(s) 1536, 1781, 2778, 6102
BseSI GKGCMC 2 cut(s) 3166, 3571
BseYI CCCAGC 2 cut(s) 1669, 5582
BsgI GTGCAG 5 cut(s) 1677, 2805, 4077, 4506, 4982
BshNI GGYRCC 2 cut(s) 275, 3566
BsiHKAI GWGCWC 7 cut(s) 1090, 1119, 1299, 2104, 2799, 3652, 3844
BsiHKCI CYCGRG 2 cut(s) 1191, 2088
BsiSI CCGG 6 cut(s) 1067, 1533, 1747, 3564, 4252, 4369
BslFI GGGAC 6 cut(s) 1208, 2092, 2479, 2957, 4593, 6210
BslI CCNNNNNNNGG 7 cut(s) 453, 2620, 3172, 3295, 4875, 6058, 6194
BsmBI CGTCTC 1 cut(s) 1559
BsmFI GGGAC 6 cut(s) 1208, 2092, 2479, 2957, 4593, 6210
BsmI GAATGC 9 cut(s) 232, 261, 889, 941, 2344, 2945, 4160, 5056, 5790
Bso31I GGTCTC 5 cut(s) 1914, 3538, 5190, 5266, 5349
BsoBI CYCGRG 2 cut(s) 1191, 2088
Bsp13I TCCGGA 1 cut(s) 1746
Bsp1407I TGTACA 1 cut(s) 4044
Bsp19I CCATGG 3 cut(s) 1230, 2543, 6063
BspEI TCCGGA 1 cut(s) 1746
BspHI TCATGA 4 cut(s) 3847, 5107, 5955, 6322
BspLI GGNNCC 9 cut(s) 72, 277, 1663, 2844, 3034, 3568, 4373, 5028, 5304
BspMAI CTGCAG 2 cut(s) 2221, 2266
BspMI ACCTGC 1 cut(s) 3095
BspQI GCTCTTC 4 cut(s) 969, 3249, 3849, 4462
BspT107I GGYRCC 2 cut(s) 275, 3566
BspTNI GGTCTC 5 cut(s) 1914, 3538, 5190, 5266, 5349
BsrBI CCGCTC 1 cut(s) 1598
BsrDI GCAATG 8 cut(s) 84, 249, 3960, 4272, 5474, 5594, 6004, 6109
BsrFI RCCGGY 1 cut(s) 1532
BsrGI TGTACA 1 cut(s) 4044
BssAI RCCGGY 1 cut(s) 1532
BssNAI GTATAC 1 cut(s) 6298
BssT1I CCWWGG 6 cut(s) 1230, 1725, 2543, 4071, 6063, 6123
Bst1107I GTATAC 1 cut(s) 6298
Bst2UI CCWGG 8 cut(s) 239, 1919, 2273, 2597, 3167, 3173, 3892, 6015
Bst6I CTCTTC 8 cut(s) 969, 3249, 3420, 3849, 4462, 5442, 5709, 6250
BstAPI GCANNNNNTGC 5 cut(s) 110, 2099, 2348, 5411, 5579
BstAUI TGTACA 1 cut(s) 4044
BstBAI YACGTR 2 cut(s) 3512, 6171
BstDSI CCRYGG 3 cut(s) 1230, 2543, 6063
BstENI CCTNNNNNAGG 1 cut(s) 6056
BstHHI GCGC 2 cut(s) 2353, 3225
BstNI CCWGG 8 cut(s) 239, 1919, 2273, 2597, 3167, 3173, 3892, 6015
BstNSI RCATGY 8 cut(s) 137, 145, 943, 1647, 3922, 4187, 4321, 5409
BstPAI GACNNNNGTC 1 cut(s) 339
BstSFI CTRYAG 6 cut(s) 915, 2217, 2262, 4197, 4203, 5157
BstSLI GKGCMC 2 cut(s) 3166, 3571
BstV2I GAAGAC 8 cut(s) 339, 650, 657, 887, 2126, 3492, 4050, 6336
BstX2I RGATCY 5 cut(s) 1867, 3535, 4285, 5302, 5660
BstXI CCANNNNNNTGG 3 cut(s) 516, 2992, 5083
BstYI RGATCY 5 cut(s) 1867, 3535, 4285, 5302, 5660
BstZ17I GTATAC 1 cut(s) 6298
BsuI GTATCC 2 cut(s) 3133, 5238
BtgI CCRYGG 3 cut(s) 1230, 2543, 6063
BtrI CACGTC 2 cut(s) 1330, 3400
BveI ACCTGC 1 cut(s) 3095
CaiI CAGNNNCTG 5 cut(s) 1480, 4341, 5296, 5324, 5579
CciI TCATGA 4 cut(s) 3847, 5107, 5955, 6322
CfoI GCGC 2 cut(s) 2353, 3225
Cfr10I RCCGGY 1 cut(s) 1532
CseI GACGC 1 cut(s) 329
CsiI ACCWGGT 1 cut(s) 6013
CspCI CAANNNNNGTGG 2 cut(s) 6197, 6232
DraI TTTAAA 1 cut(s) 66
DraIII CACNNNGTG 2 cut(s) 2069, 4255
EaeI YGGCCR 3 cut(s) 1482, 3297, 4877
Eam1104I CTCTTC 8 cut(s) 969, 3249, 3420, 3849, 4462, 5442, 5709, 6250
EarI CTCTTC 8 cut(s) 969, 3249, 3420, 3849, 4462, 5442, 5709, 6250
EciI GGCGGA 1 cut(s) 375
Ecl136II GAGCTC 3 cut(s) 1088, 3650, 3842
Eco130I CCWWGG 6 cut(s) 1230, 1725, 2543, 4071, 6063, 6123
Eco24I GRGCYC 5 cut(s) 1090, 3652, 3844, 4167, 5812
Eco31I GGTCTC 5 cut(s) 1914, 3538, 5190, 5266, 5349
Eco32I GATATC 2 cut(s) 646, 1636
Eco47I GGWCC 4 cut(s) 3169, 4371, 5173, 5694
Eco53kI GAGCTC 3 cut(s) 1088, 3650, 3842
Eco57I CTGAAG 9 cut(s) 2307, 2387, 2580, 2603, 2622, 3966, 4146, 5793, 5949
Eco88I CYCGRG 2 cut(s) 1191, 2088
EcoICRI GAGCTC 3 cut(s) 1088, 3650, 3842
EcoNI CCTNNNNNAGG 1 cut(s) 6056
EcoO109I RGGNCCY 1 cut(s) 2606
EcoRI GAATTC 1 cut(s) 1413
EcoRII CCWGG 8 cut(s) 237, 1917, 2271, 2595, 3165, 3171, 3890, 6013
EcoRV GATATC 2 cut(s) 646, 1636
EcoT14I CCWWGG 6 cut(s) 1230, 1725, 2543, 4071, 6063, 6123
EcoT22I ATGCAT 7 cut(s) 368, 941, 2344, 2452, 3026, 4160, 4185
EcoT38I GRGCYC 5 cut(s) 1090, 3652, 3844, 4167, 5812
ErhI CCWWGG 6 cut(s) 1230, 1725, 2543, 4071, 6063, 6123
Esp3I CGTCTC 1 cut(s) 1559
FalI AAGNNNNNCTT 4 cut(s) 762, 794, 1326, 1358
FaqI GGGAC 6 cut(s) 1208, 2092, 2479, 2957, 4593, 6210
FauI CCCGC 4 cut(s) 379, 998, 2622, 6033
FauNDI CATATG 1 cut(s) 5736
FbaI TGATCA 2 cut(s) 466, 3451
FblI GTMKAC 2 cut(s) 146, 6297
FriOI GRGCYC 5 cut(s) 1090, 3652, 3844, 4167, 5812
FspAI RTGCGCAY 1 cut(s) 3224
FspI TGCGCA 1 cut(s) 3224
GlaI GCGC 2 cut(s) 2352, 3224
GsaI CCCAGC 2 cut(s) 1673, 5586
GsuI CTGGAG 5 cut(s) 634, 780, 1246, 3666, 4233
HapII CCGG 6 cut(s) 1067, 1533, 1747, 3564, 4252, 4369
HgaI GACGC 1 cut(s) 329
HhaI GCGC 2 cut(s) 2353, 3225
Hin6I GCGC 2 cut(s) 2351, 3223
HinP1I GCGC 2 cut(s) 2351, 3223
HincII GTYRAC 6 cut(s) 858, 1327, 3916, 3978, 4011, 5883
HindII GTYRAC 6 cut(s) 858, 1327, 3916, 3978, 4011, 5883
HindIII AAGCTT 7 cut(s) 771, 1202, 1360, 1739, 2572, 4646, 5422
HpaII CCGG 6 cut(s) 1067, 1533, 1747, 3564, 4252, 4369
Hpy99I CGWCG 2 cut(s) 3401, 5492
HpyCH4IV ACGT 7 cut(s) 1329, 2852, 3399, 3511, 4558, 4640, 6170
HpySE526I ACGT 7 cut(s) 1329, 2852, 3399, 3511, 4558, 4640, 6170
HspAI GCGC 2 cut(s) 2351, 3223
Kpn2I TCCGGA 1 cut(s) 1746
Ksp22I TGATCA 2 cut(s) 466, 3451
LguI GCTCTTC 4 cut(s) 969, 3249, 3849, 4462
MabI ACCWGGT 1 cut(s) 6013
MaeII ACGT 7 cut(s) 1329, 2852, 3399, 3511, 4558, 4640, 6170
MbiI CCGCTC 1 cut(s) 1598
MfeI CAATTG 5 cut(s) 402, 2360, 3576, 4143, 4500
MflI RGATCY 5 cut(s) 1867, 3535, 4285, 5302, 5660
MlsI TGGCCA 3 cut(s) 1484, 3299, 4879
MluNI TGGCCA 3 cut(s) 1484, 3299, 4879
MlyI GAGTC 7 cut(s) 1400, 1793, 3401, 3589, 3686, 3691, 5330
MmeI TCCRAC 9 cut(s) 1360, 2740, 3343, 3420, 3478, 4218, 4276, 5151, 5280
Mox20I TGGCCA 3 cut(s) 1484, 3299, 4879
Mph1103I ATGCAT 7 cut(s) 368, 941, 2344, 2452, 3026, 4160, 4185
MroI TCCGGA 1 cut(s) 1746
MroXI GAANNNNTTC 4 cut(s) 1413, 3991, 4758, 5934
MscI TGGCCA 3 cut(s) 1484, 3299, 4879
MslI CAYNNNNRTG 7 cut(s) 1103, 1678, 2955, 3062, 4188, 5004, 5081
Msp20I TGGCCA 3 cut(s) 1484, 3299, 4879
MspA1I CMGCKG 5 cut(s) 1754, 4202, 4844, 4973, 5672
MspI CCGG 6 cut(s) 1067, 1533, 1747, 3564, 4252, 4369
MunI CAATTG 5 cut(s) 402, 2360, 3576, 4143, 4500
Mva1269I GAATGC 9 cut(s) 232, 261, 889, 941, 2344, 2945, 4160, 5056, 5790
MvaI CCWGG 8 cut(s) 239, 1919, 2273, 2597, 3167, 3173, 3892, 6015
NciI CCSGG 3 cut(s) 1067, 3564, 4253
NcoI CCATGG 3 cut(s) 1230, 2543, 6063
NdeI CATATG 1 cut(s) 5736
NlaIV GGNNCC 9 cut(s) 72, 277, 1663, 2844, 3034, 3568, 4373, 5028, 5304
NmuCI GTSAC 7 cut(s) 568, 596, 1047, 1622, 1796, 3207, 6259
NsbI TGCGCA 1 cut(s) 3224
NsiI ATGCAT 7 cut(s) 368, 941, 2344, 2452, 3026, 4160, 4185
NspI RCATGY 8 cut(s) 137, 145, 943, 1647, 3922, 4187, 4321, 5409
PaeI GCATGC 3 cut(s) 943, 4187, 4321
PaeR7I CTCGAG 1 cut(s) 2088
PagI TCATGA 4 cut(s) 3847, 5107, 5955, 6322
PciSI GCTCTTC 4 cut(s) 969, 3249, 3849, 4462
PctI GAATGC 9 cut(s) 232, 261, 889, 941, 2344, 2945, 4160, 5056, 5790
PdmI GAANNNNTTC 4 cut(s) 1413, 3991, 4758, 5934
PflFI GACNNNGTC 1 cut(s) 6278
PflMI CCANNNNNTGG 2 cut(s) 3295, 4875
PleI GAGTC 7 cut(s) 1399, 1793, 3400, 3589, 3686, 3690, 5329
PpsI GAGTC 7 cut(s) 1399, 1793, 3400, 3589, 3686, 3690, 5329
Ppu21I YACGTR 2 cut(s) 3512, 6171
PshAI GACNNNNGTC 1 cut(s) 339
PshBI ATTAAT 3 cut(s) 1770, 3968, 5709
PsiI TTATAA 1 cut(s) 1310
Psp124BI GAGCTC 3 cut(s) 1090, 3652, 3844
Psp1406I AACGTT 1 cut(s) 4640
Psp6I CCWGG 8 cut(s) 237, 1917, 2271, 2595, 3165, 3171, 3890, 6013
PspFI CCCAGC 2 cut(s) 1669, 5582
PspGI CCWGG 8 cut(s) 237, 1917, 2271, 2595, 3165, 3171, 3890, 6013
PspN4I GGNNCC 9 cut(s) 72, 277, 1663, 2844, 3034, 3568, 4373, 5028, 5304
PspXI VCTCGAGB 1 cut(s) 2088
PsrI GAACNNNNNNTAC 2 cut(s) 2519, 2551
PstI CTGCAG 2 cut(s) 2221, 2266
PstNI CAGNNNCTG 5 cut(s) 1480, 4341, 5296, 5324, 5579
PsuI RGATCY 5 cut(s) 1867, 3535, 4285, 5302, 5660
PsyI GACNNNGTC 1 cut(s) 6278
PvuII CAGCTG 4 cut(s) 1754, 4202, 4973, 5672
RseI CAYNNNNRTG 7 cut(s) 1103, 1678, 2955, 3062, 4188, 5004, 5081
SacI GAGCTC 3 cut(s) 1090, 3652, 3844
SapI GCTCTTC 4 cut(s) 969, 3249, 3849, 4462
ScaI AGTACT 3 cut(s) 351, 2307, 2820
SchI GAGTC 7 cut(s) 1400, 1793, 3401, 3589, 3686, 3691, 5330
SexAI ACCWGGT 1 cut(s) 6013
SfcI CTRYAG 6 cut(s) 915, 2217, 2262, 4197, 4203, 5157
Sfr274I CTCGAG 1 cut(s) 2088
SinI GGWCC 4 cut(s) 3169, 4371, 5173, 5694
SlaI CTCGAG 1 cut(s) 2088
SmiMI CAYNNNNRTG 7 cut(s) 1103, 1678, 2955, 3062, 4188, 5004, 5081
SmlI CTYRAG 8 cut(s) 626, 1223, 1947, 2088, 3080, 3541, 4701, 5722
SmoI CTYRAG 8 cut(s) 626, 1223, 1947, 2088, 3080, 3541, 4701, 5722
SpeI ACTAGT 1 cut(s) 4603
SphI GCATGC 3 cut(s) 943, 4187, 4321
SspI AATATT 4 cut(s) 486, 997, 4612, 4824
SstI GAGCTC 3 cut(s) 1090, 3652, 3844
StyI CCWWGG 6 cut(s) 1230, 1725, 2543, 4071, 6063, 6123
TaiI ACGT 7 cut(s) 1332, 2855, 3402, 3514, 4561, 4643, 6173
TatI WGTACW 5 cut(s) 349, 2305, 2818, 4044, 4106
TauI GCSGC 3 cut(s) 287, 1598, 2228
TseFI GTSAC 7 cut(s) 568, 596, 1047, 1622, 1796, 3207, 6259
Tsp45I GTSAC 7 cut(s) 568, 596, 1047, 1622, 1796, 3207, 6259
TspGWI ACGGA 1 cut(s) 1628
Tth111I GACNNNGTC 1 cut(s) 6278
Van91I CCANNNNNTGG 2 cut(s) 3295, 4875
VpaK11BI GGWCC 4 cut(s) 3169, 4371, 5173, 5694
VspI ATTAAT 3 cut(s) 1770, 3968, 5709
XagI CCTNNNNNAGG 1 cut(s) 6056
XapI RAATTY 7 cut(s) 1413, 1612, 2637, 3346, 3726, 3988, 5606
XbaI TCTAGA 1 cut(s) 2746
XceI RCATGY 8 cut(s) 137, 145, 943, 1647, 3922, 4187, 4321, 5409
XcmI CCANNNNNNNNNTGG 2 cut(s) 1677, 3526
XhoI CTCGAG 1 cut(s) 2088
XmiI GTMKAC 2 cut(s) 146, 6297
XmnI GAANNNNTTC 4 cut(s) 1413, 3991, 4758, 5934
ZrmI AGTACT 3 cut(s) 351, 2307, 2820
Zsp2I ATGCAT 7 cut(s) 368, 941, 2344, 2452, 3026, 4160, 4185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.