AT1G77600

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003552
Physical Location & Seq
Reverse (-)
29152642 .. 29162448
9807 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77600.2

Sequence Viewer

Length: 4212 bp
ATGGAGAAAACTCCGACGCAGATAGTTTCCGAATTATGTTCTCGGTTGCTACAATTATCTCGTCCCAACAAGGATTCTCTGGTCAAGCTTCTACGGGAAGTTGCTAATACTCTCTCTAAAATCGACCAGCCTTCAGCTACCAACAAGGAAAAAGGTTTGAAACTGATAGAAGCTGAGTTAAGACCTCTGAAGAAGTCCATTATCAAGCATGCTCTTCTTAAAAATCGTGATAATGACGTGAGTCTTTTGGTCACTGTCTGTGTCTCCGAGCTTTTCCGGATTTTAGCACCTCATCTACCTTTTGAAGACGAGTATCTCAGGGATATCTTCACTCTCTTTATTGCTGAGTTTTCTGAGCTATCTGATACTGTAAGCCCTTATTTCTCTAAGAGGGCGAAAATCTTGGAGACAGTCTCTCGATTGAAATTTTGTCTGCTAATGCTGGACGAGGATTGCCAAGATTTAGTTCACGAGATGTTCAACATGTTTTTCTCTCTTGTTAGAGAGCACCATCAACAAAGTTTAATTAATCAGAAAAGTATGAAAACGCAGCAGCGAAAGGCTAACACGCAGCAAACACAACATAGTTTATTTAACAACATTTTAGCTATAATGAGTGATGTTCTGGAGGAAGAGGCAAATTCGTCTTTTGTCGTTGTTATTCTGGAAAATCTTGTGAAGGAAGGAGAGGATACAACTTCTGGAGCTGATAAGCTCGCCAGTTCTCTCATTGAAAGGTGTGCCGACAGGCTCGAGCCGTTAATTTGCAGCTTTTTAACATCATGTTTTATGGAAAAAGATTCCATCCAGACCAATCTCAAAGATTCTTATCACGAAATCATATTTAAGATCTCCTTAATTGCTCCCCAGATGCTGCTAGCAGTTATCCCAAAATTGACCCAGGAACTACTGACTGATCAGGTCGATGTGCGAATAAAAGCCCTTAACTTAGCTGGAAGGATTTTTGCGCAGCCTAAGCACTGTTTAAGTTCTTACGTAGAGACATATCAAGATCTTTATGCAGAGTTCTTGAGAAGATTCTCAGATAAATCTGCAGAAGTCAGAATGGCTGCATTGAAATGTGGCAAACAGTGTTACTTCGCGAATCCATCTGGAAATAAAGCATCTGGAGTTCTCACTGCTATCCAGGAACGCCTATTAGACTTTGATGATAGGGTTCGAACACAGGCTCTAATTGTTGCTTGTGATATTATGAAGTTTAATATGAAGTATGTTCCTTTGAATCTAATTTCTGAAGCCAGTGAGAGGCTTCGGGATAAGAAGATATCTGTTAGGAAGAAGGCTCTGCAGAAGCTGACAGAAGTATATCAAGATTATTGTGACAAGTGTTCAGAAGGAGACATGACAATTACTGATAATTTCGAGCAAATCCCATGCAAAATCTTGCTACTTTGCTGCGAGAAAAATTGTGAAGAATTCAGGTCCCAAAATTTGGAACTTGTGCTCTCCGATGATCTTTTTCCTCGCCTTCTTCCCGTTGAAGAGAGGATGCGGCACTGGGTCCAATGCTTTGCCATTATGAATCATATCCACTTGAAGTCACTCAATTCAATTTTATCTCAGAAAAGAAGGTTGCAAAATGAATTGCGGCATTGCCTAACCCTCTGGAGGAAAGCTAAGGTAGACAATATAGAAGAAGCACAAAGAAAGAAAAAGAGCTACTTCGTTAAGCTGTCAGCATGCTTTCCAGATGCTTCTGAGGCAGAAGATCTCTTTGAAAAATTAGACCGAATGAGGGATGCATCAATTTTTGATGTCTTAACTTTACTGCTGGAGGAACTGTCATCCACAAATGCCCAGATTATTAAAGAGAAGTTTCTCAAGATGATTGGTGTTAAACATTCTCTTTTCGAGTTCTTGCGCATACTTTCTACAAAATGTTCGCCTAGTATATTTAGCTCAGAGCATGTTCAGTGTCTTTTGAATCAGCTTTGTGGCAGCACCTCTGCCAACACGCAATTGAAGGCTCCTTCCATCAAACTTCTTCTGGTAATTCTCAACATGTTCCCTTCTTACCTGAGAGGTTCGGAAAAGCAGTTTCTGAAGCTGCTAGAGGAGAATGATTCAGCTGCTGATGAGCTAATTGTAGTTTTATCAAAGGCAGCTCCTTATATTTCTGTCAACTTCGGTGACTATTACCCTGTTCTGGAGAAGGTTTGTCTGGAGGGTACTCGTTCTCAGACCAAATGTGCGGTTTCTGCCATCAGTTCATTGGCTGGATCATCTGAAAAATCAGTCTTTTCTGAGTTATGCGAGATGCTCATGGATTCTTTGCTGTGTGGACGGAACATTCCAACAACTTTGCAGTCTTTGGCGTGTGTTGGGCAATATTCTGTTTTGGAATATGACAATATATATGAAGATATCACCAGCTACATATACCGGGTTTTTCAAGCGGAACCATCTGACAATCAACTCCCTTGTGATCAGTCTTCTGGTTGTTGCAACTCTTGCAAGCTAAAGATATATGGGCTCAAAACACTTGTGAAAAGTTTCTTGCCTCGTCATGGTCAAGTCGTAAGGAAAATTGACGACTTGCTAAACATTTTGAAGAAAACATTAAAATCACAAGGACACGATGGCATCAAATCATGCGAGGACACAGGAGCTAATGTTAGACTGGCTGCTGCTAAAGCTGTTCTATTGCTTTCAAGGAAATGGGACCTTCATATTTCTCCTGAAGTTTTCCGTCTTACCATTTTGATGGCAAAGGATTCCAACGCCTTTATTACCAAGACTTTTCTCACCAAGTTGTACAAGCTGTTGACGGAACATATGATACCCAGTAGATATGCATGTGCTTTTTCGTTTTCTTTATCTAGTCCATGCAGAGATCTGCACGATGATTCGTTTAGATACATCAATGGATTCATCAACAAGGCTACAAGAGAATCTCGGACATGTCGGGATTTAGATCAAGGGGAATCACTCACTGATAGTCCAGTTTACATGACAGTGTTCCTAATTCATGTTCTTGCACATGACCCAGAGTTCCCTTCAGAAGACTGCAGGGACGAGCATATCTATGCTCGCTTTTGTGGCCCTCTGTTCTCGGTCTTACAGGTGTTACTTAGTATCAACAATAATGGTTTTACCATCAAGGAAACCGCCCCTTTTTTGTTTTGTATCTTCCGTGCAATTAAAAGAGCTGAAGATGCAGTTGATTCTCGTAAAACGCCTAGGCTGCACATTCTAGCCGATATTGGTTACTCAGCTGTCAACATATTAAATAGTATTGTGGTCACTTCTCCACAAGCGCCACGGTCAATTTTGCTGCCATCATCGTTGTACAGCCTCACTAGCATAACGGATAACCAGAATAAGGCTAAGTCTCGTACCCGAAATGCGCTGGAACAGAGTTTCATTGAAAGAATAGTTCATATATTCCAATCACAGATCTCCATGCATGATCAGAGGTGTCAAAAAGATAGCCTAGCAGTTGGTTCGGAAGATAAAGTTTTACCTCCATTATTAGGCAATCAAATCGAAACGTCGATTACTGGTTCAACCGAAGCCAGCCAAAATAACACGAGGTGTAGCAGGAAGCGAACTCATCTGGGAGAACATATTTCTTGTAACTCCTTATCTTTGAGAACAGTTGAGTCGGAAATTCCAATAAAGAAGCTAGAAAGACACACTACTTGTGCAAAGGAGAGTGTCAAAGCAAGTGTAAGCAATAAAATTACATCTTCCAAACACTCCGGGGTAGTATCTGCTTTAAAAGATATTAGCAACCACGGCGAAGCAATCATTGGGCAGCGGATTAAATTACTCTCTCCCACAGATGGATGTTTCTATCCTGGCACTGTTGAGAAGTTTAATTCTAAAAGCAACTCTCACAAGATTATTTTCGACAATGGGGATGTTGAATTGGTTTGCTTGGATAGCGAGAGCTGGGAGACCCTGAGCCATGAATCCATGGGGCAACAGGAACGATTGGGAAAGGAGACAGAATCTTATGGTTCTCGGAATTGCGTCCCTGAAATTAGTCATACGCTTGCAAAGGTTACTGCCCAAAAGCAGACAACTACAACAAAGCAGCAGAACAAAAAGGTGCCTGCCAAATTGAATCCTCCAGCTGCAAAATCGAAAAAAGGAAATTCGGATTCTGGAGAAGGATCAGTTTCAGAAGTGACCGACACAAGTGACAATATTGGACCAAGAAGAAGTCGGAGACAGAGAATTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1403

Amino Acids

158.37

Weight (kDa)

8.25

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 25 - 1105 2.3e-260 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 969, 1883
AccB1I GGYRCC 1 cut(s) 4075
AccB7I CCANNNNNTGG 1 cut(s) 1453
AccI GTMKAC 1 cut(s) 1644
AccII CGCG 1 cut(s) 1103
AccIII TCCGGA 1 cut(s) 276
AciI CCGC 6 cut(s) 1513, 1609, 2213, 2417, 3129, 3781
AclWI GGATC 2 cut(s) 2248, 4147
AcsI RAATTY 7 cut(s) 425, 640, 1436, 1450, 3630, 4120, 4203
AcuI CTGAAG 7 cut(s) 117, 209, 1275, 2084, 2721, 3003, 3192
AdeI CACNNNGTG 1 cut(s) 3555
AfaI GTAC 4 cut(s) 2191, 2777, 3311, 3358
AfiI CCNNNNNNNGG 9 cut(s) 1266, 1453, 1629, 1756, 2167, 2528, 3343, 3494, 3806
AflIII ACRYGT 3 cut(s) 483, 2022, 2921
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 3 cut(s) 900, 1146, 3820
AjuI GAANNNNNNNTTGG 4 cut(s) 450, 482, 3756, 3788
Alw21I GWGCWC 2 cut(s) 510, 1467
Alw26I GTCTC 9 cut(s) 268, 401, 418, 995, 1353, 3357, 3914, 3962, 4189
AlwI GGATC 2 cut(s) 2248, 4147
AlwNI CAGNNNCTG 4 cut(s) 874, 1315, 2062, 2093
Ama87I CYCGRG 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 1 cut(s) 3061
ApoI RAATTY 7 cut(s) 425, 640, 1436, 1450, 3630, 4120, 4203
AseI ATTAAT 1 cut(s) 528
Asp700I GAANNNNTTC 1 cut(s) 2513
AspA2I CCTAGG 1 cut(s) 3200
AspLEI GCGC 4 cut(s) 970, 1884, 3280, 3370
AspS9I GGNCC 5 cut(s) 1443, 1522, 2683, 3062, 4178
AsuC2I CCSGG 2 cut(s) 2405, 3724
AsuHPI GGTGA 3 cut(s) 2162, 2380, 2758
AsuII TTCGAA 1 cut(s) 1180
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 4 cut(s) 1443, 1522, 2683, 4178
AvrII CCTAGG 1 cut(s) 3200
BanI GGYRCC 1 cut(s) 4075
BanII GRGCYC 1 cut(s) 2496
BauI CACGAG 2 cut(s) 470, 3550
BbsI GAAGAC 3 cut(s) 312, 2445, 3030
Bbv12I GWGCWC 2 cut(s) 510, 1467
BceAI ACGGC 2 cut(s) 742, 3775
BcgI CGANNNNNNTGC 4 cut(s) 3447, 3481, 3487, 3521
BciT130I CCWGG 3 cut(s) 902, 1148, 3822
BciVI GTATCC 1 cut(s) 685
BclI TGATCA 3 cut(s) 916, 2446, 3430
BcnI CCSGG 2 cut(s) 2405, 3724
BcoDI GTCTC 9 cut(s) 268, 401, 418, 995, 1353, 3357, 3914, 3962, 4189
BfaI CTAG 9 cut(s) 878, 1908, 2072, 2841, 3201, 3215, 3321, 3455, 3647
BfmI CTRYAG 3 cut(s) 1053, 1307, 3028
BfoI RGCGCY 1 cut(s) 3281
BfuI GTATCC 1 cut(s) 685
BglII AGATCT 5 cut(s) 849, 1012, 1729, 2854, 3417
BlnI CCTAGG 1 cut(s) 3200
Bme1390I CCNGG 5 cut(s) 902, 1148, 2405, 3724, 3822
Bme18I GGWCC 4 cut(s) 1443, 1522, 2683, 4178
BmeT110I CYCGRG 1 cut(s) 752
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 5 cut(s) 1443, 1522, 2683, 3062, 4178
BmiI GGNNCC 6 cut(s) 1445, 1523, 1989, 2421, 2684, 4077
BmrFI CCNGG 5 cut(s) 902, 1148, 2405, 3724, 3822
BmrI ACTGGG 2 cut(s) 1528, 2799
BmsI GCATC 9 cut(s) 861, 1133, 1500, 1702, 1750, 1772, 2268, 2613, 3166
BmtI GCTAGC 1 cut(s) 881
BmuI ACTGGG 2 cut(s) 1528, 2799
BoxI GACNNNNGTC 1 cut(s) 240
BpiI GAAGAC 3 cut(s) 312, 2445, 3030
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpmI CTGGAG 9 cut(s) 647, 723, 1149, 1648, 1814, 2189, 2204, 4080, 4152
Bpu10I CCTNAGC 3 cut(s) 975, 1638, 3926
Bpu14I TTCGAA 1 cut(s) 1180
BpuEI CTTGAG 2 cut(s) 1051, 1826
BpuMI CCSGG 2 cut(s) 2405, 3724
BsaAI YACGTR 1 cut(s) 997
BsaBI GATNNNNATC 2 cut(s) 828, 2934
BsaI GGTCTC 1 cut(s) 3914
BsaJI CCNNGG 6 cut(s) 900, 3200, 3281, 3723, 3757, 3939
BsaWI WCCGGW 1 cut(s) 276
BsaXI ACNNNNNCTCC 4 cut(s) 1787, 1817, 2619, 2649
Bsc4I CCNNNNNNNGG 9 cut(s) 1266, 1453, 1629, 1756, 2167, 2528, 3343, 3494, 3806
Bse1I ACTGG 7 cut(s) 720, 1260, 1523, 2646, 2805, 2963, 3526
Bse3DI GCAATG 1 cut(s) 1612
Bse8I GATNNNNATC 2 cut(s) 828, 2934
BseAI TCCGGA 1 cut(s) 276
BseBI CCWGG 3 cut(s) 902, 1148, 3822
BseDI CCNNGG 6 cut(s) 900, 3200, 3281, 3723, 3757, 3939
BseGI GGATG 6 cut(s) 804, 1515, 1765, 1805, 3815, 3889
BseJI GATNNNNATC 2 cut(s) 828, 2934
BseLI CCNNNNNNNGG 9 cut(s) 1266, 1453, 1629, 1756, 2167, 2528, 3343, 3494, 3806
BseMI GCAATG 1 cut(s) 1612
BseNI ACTGG 7 cut(s) 720, 1260, 1523, 2646, 2805, 2963, 3526
BseRI GAGGAG 1 cut(s) 2090
BseYI CCCAGC 1 cut(s) 3915
BsgI GTGCAG 2 cut(s) 2843, 3191
Bsh1236I CGCG 1 cut(s) 1103
BshFI GGCC 1 cut(s) 3063
BshNI GGYRCC 1 cut(s) 4075
BsiHKAI GWGCWC 2 cut(s) 510, 1467
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 3 cut(s) 277, 2404, 3723
BslFI GGGAC 5 cut(s) 48, 1429, 2696, 3047, 3983
BslI CCNNNNNNNGG 9 cut(s) 1266, 1453, 1629, 1756, 2167, 2528, 3343, 3494, 3806
BsmAI GTCTC 9 cut(s) 268, 401, 418, 995, 1353, 3357, 3914, 3962, 4189
BsmFI GGGAC 5 cut(s) 48, 1429, 2696, 3047, 3983
BsnI GGCC 1 cut(s) 3063
Bso31I GGTCTC 1 cut(s) 3914
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 1 cut(s) 1180
Bsp1286I GDGCHC 3 cut(s) 510, 1467, 2496
Bsp13I TCCGGA 1 cut(s) 276
Bsp1407I TGTACA 2 cut(s) 2775, 3309
Bsp19I CCATGG 1 cut(s) 3939
Bsp68I TCGCGA 1 cut(s) 1103
BspACI CCGC 6 cut(s) 1513, 1609, 2213, 2417, 3129, 3781
BspANI GGCC 1 cut(s) 3063
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 1 cut(s) 1103
BspLI GGNNCC 6 cut(s) 1445, 1523, 1989, 2421, 2684, 4077
BspMAI CTGCAG 3 cut(s) 1057, 1311, 3032
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 2 cut(s) 2248, 4147
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 1180
BspT107I GGYRCC 1 cut(s) 4075
BspTNI GGTCTC 1 cut(s) 3914
BsrDI GCAATG 1 cut(s) 1612
BsrGI TGTACA 2 cut(s) 2775, 3309
BsrI ACTGG 7 cut(s) 720, 1260, 1523, 2646, 2805, 2963, 3526
BssECI CCNNGG 6 cut(s) 900, 3200, 3281, 3723, 3757, 3939
BssSI CACGAG 2 cut(s) 470, 3550
BssT1I CCWWGG 2 cut(s) 3200, 3939
Bst2BI CACGAG 2 cut(s) 470, 3550
Bst2UI CCWGG 3 cut(s) 902, 1148, 3822
Bst6I CTCTTC 3 cut(s) 219, 627, 1497
BstAPI GCANNNNNTGC 1 cut(s) 2472
BstAUI TGTACA 2 cut(s) 2775, 3309
BstBAI YACGTR 1 cut(s) 997
BstBI TTCGAA 1 cut(s) 1180
BstC8I GCNNGC 9 cut(s) 210, 717, 879, 1702, 2477, 3052, 3538, 4020, 4080
BstDSI CCRYGG 3 cut(s) 3281, 3757, 3939
BstF5I GGATG 6 cut(s) 804, 1515, 1765, 1805, 3815, 3889
BstFNI CGCG 1 cut(s) 1103
BstH2I RGCGCY 1 cut(s) 3281
BstHHI GCGC 4 cut(s) 970, 1884, 3280, 3370
BstMAI GTCTC 9 cut(s) 268, 401, 418, 995, 1353, 3357, 3914, 3962, 4189
BstNI CCWGG 3 cut(s) 902, 1148, 3822
BstNSI RCATGY 7 cut(s) 212, 487, 1704, 1931, 2026, 2820, 2925
BstPAI GACNNNNGTC 1 cut(s) 240
BstSCI CCNGG 5 cut(s) 900, 1146, 2403, 3722, 3820
BstSFI CTRYAG 3 cut(s) 1053, 1307, 3028
BstSNI TACGTA 1 cut(s) 997
BstUI CGCG 1 cut(s) 1103
BstV2I GAAGAC 3 cut(s) 312, 2445, 3030
BstX2I RGATCY 5 cut(s) 849, 1012, 1729, 2854, 3417
BstXI CCANNNNNNTGG 1 cut(s) 2725
BstYI RGATCY 5 cut(s) 849, 1012, 1729, 2854, 3417
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 1 cut(s) 3063
BtgI CCRYGG 3 cut(s) 3281, 3757, 3939
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 6 cut(s) 804, 1515, 1765, 1805, 3815, 3889
BtsI GCAGTG 1 cut(s) 1137
BtuMI TCGCGA 1 cut(s) 1103
Cac8I GCNNGC 9 cut(s) 210, 717, 879, 1702, 2477, 3052, 3538, 4020, 4080
CaiI CAGNNNCTG 4 cut(s) 874, 1315, 2062, 2093
CfoI GCGC 4 cut(s) 970, 1884, 3280, 3370
Cfr13I GGNCC 5 cut(s) 1443, 1522, 2683, 3062, 4178
CseI GACGC 2 cut(s) 25, 3985
Csp6I GTAC 4 cut(s) 2190, 2776, 3310, 3357
CviQI GTAC 4 cut(s) 2190, 2776, 3310, 3357
DraI TTTAAA 1 cut(s) 3741
DraIII CACNNNGTG 1 cut(s) 3555
Eam1104I CTCTTC 3 cut(s) 219, 627, 1497
EarI CTCTTC 3 cut(s) 219, 627, 1497
Eco105I TACGTA 1 cut(s) 997
Eco130I CCWWGG 2 cut(s) 3200, 3939
Eco24I GRGCYC 1 cut(s) 2496
Eco31I GGTCTC 1 cut(s) 3914
Eco32I GATATC 3 cut(s) 325, 1287, 2386
Eco47I GGWCC 4 cut(s) 1443, 1522, 2683, 4178
Eco57I CTGAAG 7 cut(s) 117, 209, 1275, 2084, 2721, 3003, 3192
Eco88I CYCGRG 1 cut(s) 752
EcoO109I RGGNCCY 2 cut(s) 1443, 2683
EcoRI GAATTC 1 cut(s) 1436
EcoRII CCWGG 3 cut(s) 900, 1146, 3820
EcoRV GATATC 3 cut(s) 325, 1287, 2386
EcoT14I CCWWGG 2 cut(s) 3200, 3939
EcoT22I ATGCAT 3 cut(s) 1765, 2818, 3429
EcoT38I GRGCYC 1 cut(s) 2496
ErhI CCWWGG 2 cut(s) 3200, 3939
FalI AAGNNNNNCTT 4 cut(s) 839, 871, 1667, 1699
FaqI GGGAC 5 cut(s) 48, 1429, 2696, 3047, 3983
FauNDI CATATG 1 cut(s) 2796
FbaI TGATCA 3 cut(s) 916, 2446, 3430
FblI GTMKAC 1 cut(s) 1644
FokI GGATG 6 cut(s) 791, 1522, 1772, 1792, 3822, 3896
FriOI GRGCYC 1 cut(s) 2496
FspBI CTAG 9 cut(s) 878, 1908, 2072, 2841, 3201, 3215, 3321, 3455, 3647
FspI TGCGCA 2 cut(s) 969, 1883
GlaI GCGC 4 cut(s) 969, 1883, 3279, 3369
GsaI CCCAGC 1 cut(s) 3919
GsuI CTGGAG 9 cut(s) 647, 723, 1149, 1648, 1814, 2189, 2204, 4080, 4152
HaeII RGCGCY 1 cut(s) 3281
HaeIII GGCC 1 cut(s) 3063
HapII CCGG 3 cut(s) 277, 2404, 3723
HgaI GACGC 2 cut(s) 25, 3985
HhaI GCGC 4 cut(s) 970, 1884, 3280, 3370
Hin6I GCGC 4 cut(s) 968, 1882, 3278, 3368
HinP1I GCGC 4 cut(s) 968, 1882, 3278, 3368
HincII GTYRAC 3 cut(s) 2143, 2787, 3241
HindII GTYRAC 3 cut(s) 2143, 2787, 3241
HindIII AAGCTT 1 cut(s) 86
HpaII CCGG 3 cut(s) 277, 2404, 3723
HphI GGTGA 3 cut(s) 2162, 2380, 2758
Hpy166II GTNNAC 7 cut(s) 469, 1645, 2143, 2303, 2787, 2968, 3241
Hpy8I GTNNAC 7 cut(s) 469, 1645, 2143, 2303, 2787, 2968, 3241
Hpy99I CGWCG 2 cut(s) 19, 3517
HpyCH4IV ACGT 3 cut(s) 237, 996, 3512
HpySE526I ACGT 3 cut(s) 237, 996, 3512
HspAI GCGC 4 cut(s) 968, 1882, 3278, 3368
Kpn2I TCCGGA 1 cut(s) 276
Ksp22I TGATCA 3 cut(s) 916, 2446, 3430
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 5 cut(s) 704, 868, 1993, 2131, 2627
LweI GCATC 9 cut(s) 861, 1133, 1500, 1702, 1750, 1772, 2268, 2613, 3166
MaeI CTAG 9 cut(s) 878, 1908, 2072, 2841, 3201, 3215, 3321, 3455, 3647
MaeII ACGT 3 cut(s) 237, 996, 3512
MfeI CAATTG 1 cut(s) 1979
MflI RGATCY 5 cut(s) 849, 1012, 1729, 2854, 3417
MhlI GDGCHC 3 cut(s) 510, 1467, 2496
MlyI GAGTC 2 cut(s) 250, 3632
MmeI TCCRAC 5 cut(s) 38, 2339, 2763, 3606, 4172
Mph1103I ATGCAT 3 cut(s) 1765, 2818, 3429
MroI TCCGGA 1 cut(s) 276
MroXI GAANNNNTTC 1 cut(s) 2513
MslI CAYNNNNRTG 4 cut(s) 1078, 2723, 2975, 3045
MspA1I CMGCKG 4 cut(s) 2090, 3236, 3781, 4100
MspI CCGG 3 cut(s) 277, 2404, 3723
MspR9I CCNGG 5 cut(s) 902, 1148, 2405, 3724, 3822
MunI CAATTG 1 cut(s) 1979
MvaI CCWGG 3 cut(s) 902, 1148, 3822
MvnI CGCG 1 cut(s) 1103
NciI CCSGG 2 cut(s) 2405, 3724
NcoI CCATGG 1 cut(s) 3939
NdeI CATATG 1 cut(s) 2796
NheI GCTAGC 1 cut(s) 877
NlaIV GGNNCC 6 cut(s) 1445, 1523, 1989, 2421, 2684, 4077
NmuCI GTSAC 7 cut(s) 250, 1340, 1560, 2150, 3262, 4153, 4166
NruI TCGCGA 1 cut(s) 1103
NsbI TGCGCA 2 cut(s) 969, 1883
NsiI ATGCAT 3 cut(s) 1765, 2818, 3429
NspI RCATGY 7 cut(s) 212, 487, 1704, 1931, 2026, 2820, 2925
NspV TTCGAA 1 cut(s) 1180
PacI TTAATTAA 1 cut(s) 528
PaeI GCATGC 2 cut(s) 212, 1704
PaeR7I CTCGAG 1 cut(s) 752
PciI ACATGT 3 cut(s) 483, 2022, 2921
PciSI GCTCTTC 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 2513
PflMI CCANNNNNTGG 1 cut(s) 1453
PfoI TCCNGGA 1 cut(s) 1146
PleI GAGTC 2 cut(s) 249, 3631
PpsI GAGTC 2 cut(s) 249, 3631
Ppu21I YACGTR 1 cut(s) 997
PpuMI RGGWCCY 2 cut(s) 1443, 2683
PscI ACATGT 3 cut(s) 483, 2022, 2921
PshAI GACNNNNGTC 1 cut(s) 240
PshBI ATTAAT 1 cut(s) 528
Psp5II RGGWCCY 2 cut(s) 1443, 2683
Psp6I CCWGG 3 cut(s) 900, 1146, 3820
PspFI CCCAGC 1 cut(s) 3915
PspGI CCWGG 3 cut(s) 900, 1146, 3820
PspN4I GGNNCC 6 cut(s) 1445, 1523, 1989, 2421, 2684, 4077
PspPI GGNCC 5 cut(s) 1443, 1522, 2683, 3062, 4178
PspPPI RGGWCCY 2 cut(s) 1443, 2683
PspXI VCTCGAGB 1 cut(s) 752
PsrI GAACNNNNNNTAC 2 cut(s) 2784, 2816
PstI CTGCAG 3 cut(s) 1057, 1311, 3032
PstNI CAGNNNCTG 4 cut(s) 874, 1315, 2062, 2093
PsuI RGATCY 5 cut(s) 849, 1012, 1729, 2854, 3417
PvuII CAGCTG 3 cut(s) 2090, 3236, 4100
RruI TCGCGA 1 cut(s) 1103
RsaI GTAC 4 cut(s) 2191, 2777, 3311, 3358
RsaNI GTAC 4 cut(s) 2190, 2776, 3310, 3357
RseI CAYNNNNRTG 4 cut(s) 1078, 2723, 2975, 3045
SapI GCTCTTC 1 cut(s) 219
Sau96I GGNCC 5 cut(s) 1443, 1522, 2683, 3062, 4178
SchI GAGTC 2 cut(s) 250, 3632
ScrFI CCNGG 5 cut(s) 902, 1148, 2405, 3724, 3822
SduI GDGCHC 3 cut(s) 510, 1467, 2496
SfaNI GCATC 9 cut(s) 861, 1133, 1500, 1702, 1750, 1772, 2268, 2613, 3166
SfcI CTRYAG 3 cut(s) 1053, 1307, 3028
Sfr274I CTCGAG 1 cut(s) 752
SfuI TTCGAA 1 cut(s) 1180
SinI GGWCC 4 cut(s) 1443, 1522, 2683, 4178
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 4 cut(s) 1078, 2723, 2975, 3045
SmlI CTYRAG 3 cut(s) 752, 1030, 1841
SmoI CTYRAG 3 cut(s) 752, 1030, 1841
SnaBI TACGTA 1 cut(s) 997
SphI GCATGC 2 cut(s) 212, 1704
SsiI CCGC 6 cut(s) 1513, 1609, 2213, 2417, 3129, 3781
SspI AATATT 2 cut(s) 2351, 4174
SspMI CTAG 9 cut(s) 878, 1908, 2072, 2841, 3201, 3215, 3321, 3455, 3647
StyD4I CCNGG 5 cut(s) 900, 1146, 2403, 3722, 3820
StyI CCWWGG 2 cut(s) 3200, 3939
TaiI ACGT 3 cut(s) 240, 999, 3515
TaqII GACCGA 3 cut(s) 1764, 3064, 4172
TatI WGTACW 2 cut(s) 2775, 3309
TauI GCSGC 2 cut(s) 1516, 1612
TseFI GTSAC 7 cut(s) 250, 1340, 1560, 2150, 3262, 4153, 4166
Tsp45I GTSAC 7 cut(s) 250, 1340, 1560, 2150, 3262, 4153, 4166
TspGWI ACGGA 5 cut(s) 2320, 2699, 2804, 3143, 3344
Van91I CCANNNNNTGG 1 cut(s) 1453
VpaK11BI GGWCC 4 cut(s) 1443, 1522, 2683, 4178
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 7 cut(s) 425, 640, 1436, 1450, 3630, 4120, 4203
XceI RCATGY 7 cut(s) 212, 487, 1704, 1931, 2026, 2820, 2925
XcmI CCANNNNNNNNNTGG 1 cut(s) 2230
XhoI CTCGAG 1 cut(s) 752
XmaJI CCTAGG 1 cut(s) 3200
XmiI GTMKAC 1 cut(s) 1644
XmnI GAANNNNTTC 1 cut(s) 2513
XspI CTAG 9 cut(s) 878, 1908, 2072, 2841, 3201, 3215, 3321, 3455, 3647
Zsp2I ATGCAT 3 cut(s) 1765, 2818, 3429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.