MD15G1350300.v1.1

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003552
Physical Location & Seq
Reverse (-)
41509885 .. 41532790
22906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1350300.v1.1.491

Sequence Viewer

Length: 4116 bp
ATGGCCGAGTCCGCGCTGCAACTCGTCTCCGAAATCGGCACCCACCTCCGCCGCCAAACGCGCCCAAACAAGGACTTCATCGTCAAATCTCTCCGACAAGCTGCAAGTGCTTTGTCGCAGTTAGAACATGCTTCTTCGGCTGAAGCTTGTAAGAAGTTGGAGCCCCTGAGAGAAGCTATTGTGCATGGCTTGCTTCAGCATAGGGATAAGGACGTCAGGCTTCTTGTGGCCATCTGTGTAACTGAAATGTTTCGGGCTATGGCACCCGAACCCCCTTTTGCGGACAAATATCTAAGGAACGTTTTTAAACTCATTATCAGCACGTTCGTCGAGCTAGCTGATATGGAGAGCCCGCTCTTTTCGAGGAGGGCCAAAATAGTAGAGACTGTTGCGCGATGTAAATGTTGTGTGATCATGTTGGATGTTGACTGCAATGATCTAGTTCTCGAAATGTTCAATGCCTTCTTCTCTGTTGTGAGGCAACATCATCAGCAGAGTTTGATGAATGATATTTTGTCTATAATGGTTCATATACTAAACGAGGAAGCTTCTCAGCCACTTTTGGATGTGGTTCTACGAAATCTTGTGAAGGAGGCAAAGGATGCAGATTCTGCTTCTTCTCAGCTTGCAGTTTCTGTGATCCAAACATGTGCAGACAAACTTGAGTCCTTTGTTTGTGGGTTTCTGACATCTTGTATTTTGGATGGAGATGCTGATGGGAGTGAGCTCAAGGAATTTTACCATGAAATCATTTTTAAAATTTTTGGGTGTGCTCCTCATATGCTTCTTGCTGTCATTCCAAATTTGACTCAAGAGTTACTGACTGATCAGGTTGATGTCCGACTCAAAGCTGTTAATTTAATTGGAAAACTTTTCACACTGCCTGATCACCATATTGCACAAAGGTATCATGACCTCTTCGTTGAGTTTTTGAAAAGATTTTCGGATAAATCTGCGGACGTTAGAGTTAGTGCTCTACAGTGTGCTAAAGTTTGCTATGTGACCAACCCCTCTGGGGCGGAATCACAAGAAGTTCTCCCTGCCCTTGAAAGTCGGCTACTAGATTTTGATGATAGGGTGAGAACACAGGCAGTGATTGTTGCCTGTGATCTTGCCATGTCCAATACGAGATGCTTTCCTCCCAAAATAATATCTCAGACCACTGAAAGACTTAGGGATAAGAAGATACCCGTTAGAAAGAAAGCTTTGCAGAAGTTGATGGAAGTATATCGAGATTATTGTAACAAATGCTTTAAAGGCTCTATGACAATCAGTGACCACTTTGAACAGATTCCTTGTAAAATATTGATGCTATGCTTTGATAAAGATTGTATGGATTTCAGGTCCCAAAATATGGAGCTTGTTCTTGCAGAAGATCTATTTCCAGCTGGTCTTTCAGTTCATGAAATTACAAGGCACTGGATCCACTTGTTTTCTCTTTTCACCCCACTTCATATAAAGGCTTTGAACTCTATTTTATCTCAGAAACAAAGGTTGCAAAGTGAGATGCAAACTTATTTAGCCAATCGAAAGAAAGAGAAGGGGAGTAATTCAGAAGAGATGCAGAAGAGATATAAAGTTCAGTTCTCAAAAATGGCAGTCTCCTTTGCAGACCCTTCAAGAGCAGAAGAGTGCTTTGAAAAATTTAACCAAATGAAAGATAATAACATTTTTAATTCACTGGCTCTGTTATTGGATGACCTGCAATTTAAAGAAGCCCGTGCAAGCAGAGACAAATTTCTGAATATGATTGGCGGGAAACATCAGAATTTTGAGTTTTTACGAACACTTTCCTCAAAATGCTCTTATAATATATTCAGTGCAGCGCATGTTTGCTGTATTCTAGATGATCTATCCAGTAACAGTCCTGGAAAGAGAAACTTGGTGGCTTCTTCTGTCAGACTTCTGCTGTCAATCACCAGCTTTTTCCCAACACTCTTAAGAGGTTCAGAAGTGCAACTTCAGAAGTTATTAAAGGAGACGGACCCAATTAATGATAAGTTGATTGAAGTGTTAGCCAAAGCAGGCCCTCACATATTTGTTAAACTCAGTGAAATCTACCCCTTTCTGAAGAGGGTTTGTCTGGAGGGGACTCGCGTTCAGTCAAAATATGCTGTTTCAGCAATTGCTGCTTTAGTTGATCATTCAAAGCAATTGATTTTCCAAGATCTATGTAAGGAACTTGTGGATTCTCTACTAGTTGGGCAGAACATACCAACAGTGTTACAGTCCTTGGGGTGTCTTGCACAGCATTCTGTTTCAACATTCCAAAGCCAAGTTGGAGAGATCACCCATTATATATATCAAAAAATGTTCCAAGTGAACTCTTCAGATTTTATAGACTCTTGTGATGATGCATCCGGGTCTAGTGATTCTTGCAAATTAAAGATATATGGCCTGAAAACACTTGTCAAGAGCTTCTTGCCACACAGGGGAACTCACACCAAACAGCAAATTAATGAGCTTGAGGACTACAGCAAATGGCAAATTGATGAGCTCTGGGACATTTTGTCAACAATGCTGCAAAGGGGAGAAACTGCTGAGGGTATCACTTCATGTGAAAGTGATAAGGCTTGTATTAGATTAGCTGCTGCAAAGTCTGTTCTCCGGCTTGCTCGAAGATGGGATTTCCATATTTCTCCAGAGATCTTTCACTTTACAATTTTAATGGCAAAGGATGATTCTCCATTGGTGAGAAGATCATTTCTTGATAAAACTCACAAATTACTGAAGGAGCATGTTATACCTAGTAGATATGCATGTGCTTTCGCAATGGCCACTTCAGATTGCCTCAAGGATCTGCAGGATGATTCGTTAAAATATATTGCAGAATTTGTCAAAGATTACAGTAGAGAAGCTCAAGTGCGTCAAATCTCTGGAGTCCAAGAAGGATTAAACACTGATTTTCCAGCATACATAGTGGTGTTCTTGATCCATATTCTTGCTCATGATACAGGCTTCCCACCTGAAGACTGTCAAGATGAAAAAGCATATGCTCAGTTTTGCGGTCCACTGTTAGGTTTATTGCAGGTTTTAGTCAATGCTAGTAATGCTGATGGGGCTCTGGATGTTGCCAAGGATTCTGTCTTGTATTTGATTTGCATTTTTCGTGCAATTAAGAGAGCTGAGGATGCTGTAGACACTGAATTAACCGGTAAGCTGCATATTCTGGCAGAAATTGGGCATTCTTTTGTGACGTTAACAAATCACAATGCCCTCTCCTCATTACATGCTCCTGGGAAAATTTTTCTACCATCATCCTTATATAAATCCAATTCAAGATGCCTCACTCAATCATGTTTTGATGAGTACTTTTTCAAAAGAGTAGTTGACATATTTAAATCAAATCTCTCTTTGCCTGCCGGTGCTCTTCCTAGACGTGGGCGTAAATGTCAAGAGGATATCACACAATCTGGTGTTGTCAAGGACAGCAAACATATCGTAACATCTTCCAAGATAGTTAATTTGTGCAATGATGGGGAAGCTGAACCCCGAAAGGCTGAGAAACAAGGCACCTGTAGAGGAGGATGTAGAAGAAAACGGGATCTCTCTCCCAATGATTCTGATATTGGTTACCAGAATGGTTTATCTAAAAAATCTGATATTACTTTGGAAAAAGAAATACTTTCATCTTGTGATTCTGTGGCTACAGTCGGTGGATCAAATGTCACTGTCCAGAATATTAAAAAAAATACCATTCCATTGATGGGAAATGTTAATGTGAAAAGAAGCATTAATGTGGAATCCAATGACCCCAGATCGAATCTAAAGGGTCCTTGCAGCTTAAAGGAAATTGGTAAGAAGGCTGAGGCATTGATTGGGCAAAGGATCAAATTTTTGTCTCCAGAAGATAAATGTTTTTATTCAGGTACAGTGGATGGTTATAATACTCAAAACAATACATACAAGATCACATGTGATGGTAGCGGGGATGTTCAATTAGTATGCTTGGAAAGTAAGAGCTGGGAACCTATAAGTGAGGGTTCACTGGAGGAAAGGAAACGAAAGCTGGGGAAAAAAGCTTTTGTGGACACCTCGGCATCAGAAGTTACTAATGCAAATGAGGATGCTGTGGCTAGAAGAACTCGAAGACAAAAGAAACTAAATGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1372

Amino Acids

154.04

Weight (kDa)

8.0

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 26 - 1090 1.3e-278 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1809, 3885
AasI GACNNNNNNGTC 1 cut(s) 80
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 2 cut(s) 1710, 3019
AccB1I GGYRCC 3 cut(s) 38, 262, 3512
AccB7I CCANNNNNTGG 1 cut(s) 1354
AccBSI CCGCTC 1 cut(s) 355
AccI GTMKAC 1 cut(s) 3138
AccII CGCG 4 cut(s) 14, 61, 394, 2097
AclI AACGTT 1 cut(s) 300
AclWI GGATC 8 cut(s) 634, 1417, 1430, 2805, 2926, 3552, 3667, 3836
AcoI YGGCCR 3 cut(s) 3, 228, 2775
AcsI RAATTY 9 cut(s) 734, 759, 802, 1643, 1736, 1768, 2831, 3243, 3833
AcuI CTGAAG 8 cut(s) 162, 179, 1946, 2090, 2313, 2750, 2766, 2988
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 3311, 3871
AfiI CCNNNNNNNGG 8 cut(s) 70, 280, 1015, 1354, 2432, 3017, 3380, 3707
AflII CTTAAG 1 cut(s) 1939
AflIII ACRYGT 2 cut(s) 647, 3914
AgeI ACCGGT 1 cut(s) 3152
AhdI GACNNNNNGTC 1 cut(s) 2509
AhlI ACTAGT 1 cut(s) 2197
AjiI CACGTC 1 cut(s) 3380
AjnI CCWGG 2 cut(s) 1867, 3235
Alw21I GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
Alw26I GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3846
AlwI GGATC 8 cut(s) 634, 1417, 1430, 2805, 2926, 3552, 3667, 3836
AlwNI CAGNNNCTG 2 cut(s) 611, 635
AoxI GGCC 6 cut(s) 3, 228, 369, 2026, 2395, 2775
ApeKI GCWGC 9 cut(s) 16, 101, 1823, 2129, 2521, 2588, 2591, 3160, 3780
ApoI RAATTY 9 cut(s) 734, 759, 802, 1643, 1736, 1768, 2831, 3243, 3833
AseI ATTAAT 3 cut(s) 1992, 2457, 3735
AsiGI ACCGGT 1 cut(s) 3152
Asp700I GAANNNNTTC 3 cut(s) 249, 1290, 1789
AspLEI GCGC 4 cut(s) 16, 63, 394, 1828
AspS9I GGNCC 6 cut(s) 369, 1344, 1984, 2027, 3008, 3773
AsuC2I CCSGG 1 cut(s) 2362
AsuHPI GGTGA 6 cut(s) 881, 1090, 1435, 1909, 2281, 2704
AsuNHI GCTAGC 1 cut(s) 334
AvaII GGWCC 4 cut(s) 1344, 1984, 3008, 3773
BaeI ACNNNNGTAYC 2 cut(s) 890, 923
BalI TGGCCA 2 cut(s) 230, 2777
BamHI GGATCC 1 cut(s) 1422
BanI GGYRCC 3 cut(s) 38, 262, 3512
BanII GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
BbsI GAAGAC 2 cut(s) 2976, 4096
Bbv12I GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
BbvCI CCTCAGC 3 cut(s) 2541, 3126, 3807
BbvI GCAGC 9 cut(s) 3, 88, 1835, 2116, 2508, 2575, 2578, 3147, 3792
BcgI CGANNNNNNTGC 4 cut(s) 310, 344, 3421, 3455
BciT130I CCWGG 2 cut(s) 1869, 3237
BclI TGATCA 4 cut(s) 411, 826, 886, 2140
BcnI CCSGG 1 cut(s) 2362
BcoDI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3846
BcuI ACTAGT 1 cut(s) 2197
BfmI CTRYAG 6 cut(s) 977, 2473, 2801, 3135, 3517, 3648
BfrI CTTAAG 1 cut(s) 1939
BfuAI ACCTGC 2 cut(s) 1710, 3019
BglII AGATCT 3 cut(s) 1375, 2167, 2646
BmcAI AGTACT 1 cut(s) 3311
Bme1390I CCNGG 3 cut(s) 1869, 2362, 3237
Bme18I GGWCC 4 cut(s) 1344, 1984, 3008, 3773
BmeRI GACNNNNNGTC 1 cut(s) 2509
BmgBI CACGTC 1 cut(s) 3380
BmgT120I GGNCC 6 cut(s) 369, 1344, 1984, 2027, 3008, 3773
BmiI GGNNCC 9 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3774, 3969
BmrFI CCNGG 3 cut(s) 1869, 2362, 3237
BmtI GCTAGC 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 2976, 4096
BpmI CTGGAG 5 cut(s) 2105, 2625, 2898, 3828, 4010
Bpu10I CCTNAGC 3 cut(s) 2541, 3126, 3807
BpuEI CTTGAG 6 cut(s) 683, 713, 795, 2486, 2777, 2844
BpuMI CCSGG 1 cut(s) 2362
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 2232, 3075, 3236, 4035
BsaWI WCCGGW 1 cut(s) 3152
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 280, 1015, 1354, 2432, 3017, 3380, 3707
Bse118I RCCGGY 2 cut(s) 3152, 3362
Bse1I ACTGG 4 cut(s) 1424, 1686, 1857, 3993
Bse3DI GCAATG 3 cut(s) 439, 2778, 3478
BseBI CCWGG 2 cut(s) 1869, 3237
BseDI CCNNGG 4 cut(s) 2232, 3075, 3236, 4035
BseLI CCNNNNNNNGG 8 cut(s) 70, 280, 1015, 1354, 2432, 3017, 3380, 3707
BseMI GCAATG 3 cut(s) 439, 2778, 3478
BseNI ACTGG 4 cut(s) 1424, 1686, 1857, 3993
BseRI GAGGAG 4 cut(s) 379, 765, 3211, 3537
BseXI GCAGC 9 cut(s) 3, 88, 1835, 2116, 2508, 2575, 2578, 3147, 3792
BseYI CCCAGC 2 cut(s) 3963, 4009
BsgI GTGCAG 2 cut(s) 672, 1842
Bsh1236I CGCG 4 cut(s) 14, 61, 394, 2097
BshFI GGCC 6 cut(s) 5, 230, 371, 2028, 2397, 2777
BshNI GGYRCC 3 cut(s) 38, 262, 3512
BshTI ACCGGT 1 cut(s) 3152
BsiHKAI GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
BsiSI CCGG 4 cut(s) 2361, 2608, 3153, 3363
BslFI GGGAC 3 cut(s) 1330, 2104, 2516
BslI CCNNNNNNNGG 8 cut(s) 70, 280, 1015, 1354, 2432, 3017, 3380, 3707
BsmAI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3846
BsmBI CGTCTC 2 cut(s) 31, 1973
BsmFI GGGAC 3 cut(s) 1330, 2104, 2516
BsmI GAATGC 2 cut(s) 2251, 3184
BsnI GGCC 6 cut(s) 5, 230, 371, 2028, 2397, 2777
Bsp1286I GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
BspANI GGCC 6 cut(s) 5, 230, 371, 2028, 2397, 2777
BspFNI CGCG 4 cut(s) 14, 61, 394, 2097
BspHI TCATGA 3 cut(s) 910, 1402, 2947
BspLI GGNNCC 9 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3774, 3969
BspMAI CTGCAG 1 cut(s) 2805
BspMI ACCTGC 2 cut(s) 1710, 3019
BspOI GCTAGC 1 cut(s) 338
BspPI GGATC 8 cut(s) 634, 1417, 1430, 2805, 2926, 3552, 3667, 3836
BspQI GCTCTTC 1 cut(s) 3375
BspT107I GGYRCC 3 cut(s) 38, 262, 3512
BspTI CTTAAG 1 cut(s) 1939
BsrBI CCGCTC 1 cut(s) 355
BsrDI GCAATG 3 cut(s) 439, 2778, 3478
BsrFI RCCGGY 2 cut(s) 3152, 3362
BsrI ACTGG 4 cut(s) 1424, 1686, 1857, 3993
BssAI RCCGGY 2 cut(s) 3152, 3362
BssECI CCNNGG 4 cut(s) 2232, 3075, 3236, 4035
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2232, 3075
Bst2UI CCWGG 2 cut(s) 1869, 3237
Bst6I CTCTTC 7 cut(s) 923, 1551, 1562, 1623, 2066, 2332, 3375
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 1939
BstAPI GCANNNNNTGC 4 cut(s) 190, 602, 611, 2129
BstC8I GCNNGC 8 cut(s) 191, 336, 353, 627, 1726, 2026, 2613, 3360
BstEII GGTNACC 1 cut(s) 3572
BstFNI CGCG 4 cut(s) 14, 61, 394, 2097
BstHHI GCGC 4 cut(s) 16, 63, 394, 1828
BstMAI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3846
BstNI CCWGG 2 cut(s) 1869, 3237
BstNSI RCATGY 7 cut(s) 131, 651, 1832, 2741, 2763, 3233, 3918
BstPI GGTNACC 1 cut(s) 3572
BstSCI CCNGG 3 cut(s) 1867, 2360, 3235
BstSFI CTRYAG 6 cut(s) 977, 2473, 2801, 3135, 3517, 3648
BstUI CGCG 4 cut(s) 14, 61, 394, 2097
BstV1I GCAGC 9 cut(s) 3, 88, 1835, 2116, 2508, 2575, 2578, 3147, 3792
BstV2I GAAGAC 2 cut(s) 2976, 4096
BstX2I RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
BstYI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
BsuRI GGCC 6 cut(s) 5, 230, 371, 2028, 2397, 2777
BtgZI GCGATG 1 cut(s) 409
BtrI CACGTC 1 cut(s) 3380
BtsI GCAGTG 2 cut(s) 878, 1098
BveI ACCTGC 2 cut(s) 1710, 3019
Cac8I GCNNGC 8 cut(s) 191, 336, 353, 627, 1726, 2026, 2613, 3360
CaiI CAGNNNCTG 2 cut(s) 611, 635
CciI TCATGA 3 cut(s) 910, 1402, 2947
CfoI GCGC 4 cut(s) 16, 63, 394, 1828
Cfr10I RCCGGY 2 cut(s) 3152, 3362
Cfr13I GGNCC 6 cut(s) 369, 1344, 1984, 2027, 3008, 3773
CseI GACGC 1 cut(s) 2855
Csp6I GTAC 2 cut(s) 3310, 3870
CspAI ACCGGT 1 cut(s) 3152
CviQI GTAC 2 cut(s) 3310, 3870
DraI TTTAAA 5 cut(s) 307, 757, 1255, 1711, 3340
DrdI GACNNNNNNGTC 1 cut(s) 80
DriI GACNNNNNGTC 1 cut(s) 2509
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 3 cut(s) 3, 228, 2775
Eam1104I CTCTTC 7 cut(s) 923, 1551, 1562, 1623, 2066, 2332, 3375
Eam1105I GACNNNNNGTC 1 cut(s) 2509
EarI CTCTTC 7 cut(s) 923, 1551, 1562, 1623, 2066, 2332, 3375
EciI GGCGGA 2 cut(s) 38, 1034
Ecl136II GAGCTC 2 cut(s) 727, 2497
Eco130I CCWWGG 2 cut(s) 2232, 3075
Eco24I GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
Eco32I GATATC 1 cut(s) 3403
Eco47I GGWCC 4 cut(s) 1344, 1984, 3008, 3773
Eco53kI GAGCTC 2 cut(s) 727, 2497
Eco57I CTGAAG 8 cut(s) 162, 179, 1946, 2090, 2313, 2750, 2766, 2988
Eco91I GGTNACC 1 cut(s) 3572
EcoICRI GAGCTC 2 cut(s) 727, 2497
EcoO109I RGGNCCY 3 cut(s) 1344, 2027, 3773
EcoO65I GGTNACC 1 cut(s) 3572
EcoRII CCWGG 2 cut(s) 1867, 3235
EcoRV GATATC 1 cut(s) 3403
EcoT14I CCWWGG 2 cut(s) 2232, 3075
EcoT22I ATGCAT 2 cut(s) 2359, 2761
EcoT38I GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
ErhI CCWWGG 2 cut(s) 2232, 3075
Esp3I CGTCTC 2 cut(s) 31, 1973
FalI AAGNNNNNCTT 8 cut(s) 1865, 1897, 1944, 1976, 2405, 2437, 3609, 3641
FaqI GGGAC 3 cut(s) 1330, 2104, 2516
FauI CCCGC 3 cut(s) 360, 1748, 3920
FauNDI CATATG 2 cut(s) 780, 2992
FbaI TGATCA 4 cut(s) 411, 826, 886, 2140
FblI GTMKAC 1 cut(s) 3138
FriOI GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
GlaI GCGC 4 cut(s) 15, 62, 393, 1827
GsaI CCCAGC 2 cut(s) 3967, 4013
GsuI CTGGAG 5 cut(s) 2105, 2625, 2898, 3828, 4010
HaeIII GGCC 6 cut(s) 5, 230, 371, 2028, 2397, 2777
HapII CCGG 4 cut(s) 2361, 2608, 3153, 3363
HgaI GACGC 1 cut(s) 2855
HhaI GCGC 4 cut(s) 16, 63, 394, 1828
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 4 cut(s) 14, 61, 392, 1826
HinP1I GCGC 4 cut(s) 14, 61, 392, 1826
HincII GTYRAC 4 cut(s) 427, 2514, 3201, 3331
HindII GTYRAC 4 cut(s) 427, 2514, 3201, 3331
HindIII AAGCTT 4 cut(s) 144, 546, 1203, 4020
HpaI GTTAAC 1 cut(s) 3201
HpaII CCGG 4 cut(s) 2361, 2608, 3153, 3363
HphI GGTGA 6 cut(s) 881, 1090, 1435, 1909, 2281, 2704
Hpy166II GTNNAC 9 cut(s) 427, 2323, 2514, 3011, 3139, 3201, 3331, 3986, 4030
Hpy8I GTNNAC 9 cut(s) 427, 2323, 2514, 3011, 3139, 3201, 3331, 3986, 4030
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 7 cut(s) 472, 583, 1534, 1626, 2725, 2882, 3796
HpyCH4IV ACGT 6 cut(s) 213, 300, 323, 960, 3197, 3379
HpySE526I ACGT 6 cut(s) 213, 300, 323, 960, 3197, 3379
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 4 cut(s) 14, 61, 392, 1826
Ksp22I TGATCA 4 cut(s) 411, 826, 886, 2140
KspAI GTTAAC 1 cut(s) 3201
LguI GCTCTTC 1 cut(s) 3375
LmnI GCTCC 5 cut(s) 160, 778, 1357, 2734, 3238
Lsp1109I GCAGC 9 cut(s) 3, 88, 1835, 2116, 2508, 2575, 2578, 3147, 3792
MaeII ACGT 6 cut(s) 213, 300, 323, 960, 3197, 3379
MbiI CCGCTC 1 cut(s) 355
MfeI CAATTG 2 cut(s) 2124, 2153
MflI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
MhlI GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
MlsI TGGCCA 2 cut(s) 230, 2777
MluNI TGGCCA 2 cut(s) 230, 2777
MlyI GAGTC 7 cut(s) 17, 674, 802, 837, 2086, 2336, 2889
MmeI TCCRAC 5 cut(s) 118, 138, 399, 865, 2260
Mox20I TGGCCA 2 cut(s) 230, 2777
Mph1103I ATGCAT 2 cut(s) 2359, 2761
MroXI GAANNNNTTC 3 cut(s) 249, 1290, 1789
MscI TGGCCA 2 cut(s) 230, 2777
MslI CAYNNNNRTG 2 cut(s) 2921, 3737
Msp20I TGGCCA 2 cut(s) 230, 2777
MspA1I CMGCKG 1 cut(s) 1388
MspCI CTTAAG 1 cut(s) 1939
MspI CCGG 4 cut(s) 2361, 2608, 3153, 3363
MspR9I CCNGG 3 cut(s) 1869, 2362, 3237
MunI CAATTG 2 cut(s) 2124, 2153
Mva1269I GAATGC 2 cut(s) 2251, 3184
MvaI CCWGG 2 cut(s) 1869, 3237
MvnI CGCG 4 cut(s) 14, 61, 394, 2097
NciI CCSGG 1 cut(s) 2362
NdeI CATATG 2 cut(s) 780, 2992
NheI GCTAGC 1 cut(s) 334
NlaIV GGNNCC 9 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3774, 3969
NmeAIII GCCGAG 2 cut(s) 31, 4016
NmuCI GTSAC 4 cut(s) 1000, 1274, 3193, 3667
NsiI ATGCAT 2 cut(s) 2359, 2761
NspI RCATGY 7 cut(s) 131, 651, 1832, 2741, 2763, 3233, 3918
PagI TCATGA 3 cut(s) 910, 1402, 2947
PciI ACATGT 2 cut(s) 647, 3914
PciSI GCTCTTC 1 cut(s) 3375
PctI GAATGC 2 cut(s) 2251, 3184
PdmI GAANNNNTTC 3 cut(s) 249, 1290, 1789
PflMI CCANNNNNTGG 1 cut(s) 1354
PfoI TCCNGGA 1 cut(s) 1867
PinAI ACCGGT 1 cut(s) 3152
PleI GAGTC 7 cut(s) 16, 673, 802, 837, 2086, 2336, 2888
PpsI GAGTC 7 cut(s) 16, 673, 802, 837, 2086, 2336, 2888
PpuMI RGGWCCY 2 cut(s) 1344, 3773
PscI ACATGT 2 cut(s) 647, 3914
PshBI ATTAAT 3 cut(s) 1992, 2457, 3735
PsiI TTATAA 2 cut(s) 1809, 3885
Psp124BI GAGCTC 2 cut(s) 729, 2499
Psp1406I AACGTT 1 cut(s) 300
Psp5II RGGWCCY 2 cut(s) 1344, 3773
Psp6I CCWGG 2 cut(s) 1867, 3235
PspEI GGTNACC 1 cut(s) 3572
PspFI CCCAGC 2 cut(s) 3963, 4009
PspGI CCWGG 2 cut(s) 1867, 3235
PspN4I GGNNCC 9 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3774, 3969
PspPI GGNCC 6 cut(s) 369, 1344, 1984, 2027, 3008, 3773
PspPPI RGGWCCY 2 cut(s) 1344, 3773
PstI CTGCAG 1 cut(s) 2805
PstNI CAGNNNCTG 2 cut(s) 611, 635
PsuI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 2 cut(s) 3311, 3871
RsaNI GTAC 2 cut(s) 3310, 3870
RseI CAYNNNNRTG 2 cut(s) 2921, 3737
SacI GAGCTC 2 cut(s) 729, 2499
SapI GCTCTTC 1 cut(s) 3375
Sau96I GGNCC 6 cut(s) 369, 1344, 1984, 2027, 3008, 3773
ScaI AGTACT 1 cut(s) 3311
SchI GAGTC 7 cut(s) 17, 674, 802, 837, 2086, 2336, 2889
ScrFI CCNGG 3 cut(s) 1869, 2362, 3237
SduI GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
SfcI CTRYAG 6 cut(s) 977, 2473, 2801, 3135, 3517, 3648
SinI GGWCC 4 cut(s) 1344, 1984, 3008, 3773
SmiI ATTTAAAT 1 cut(s) 3340
SmiMI CAYNNNNRTG 2 cut(s) 2921, 3737
SmlI CTYRAG 7 cut(s) 662, 728, 810, 1939, 2465, 2792, 2859
SmoI CTYRAG 7 cut(s) 662, 728, 810, 1939, 2465, 2792, 2859
SpeI ACTAGT 1 cut(s) 2197
SspI AATATT 2 cut(s) 1305, 3682
SstI GAGCTC 2 cut(s) 729, 2499
StyD4I CCNGG 3 cut(s) 1867, 2360, 3235
StyI CCWWGG 2 cut(s) 2232, 3075
SwaI ATTTAAAT 1 cut(s) 3340
TaiI ACGT 6 cut(s) 216, 303, 326, 963, 3200, 3382
TaqI TCGA 8 cut(s) 330, 362, 447, 1231, 1528, 2617, 3761, 4087
TatI WGTACW 1 cut(s) 3309
TauI GCSGC 1 cut(s) 54
TseFI GTSAC 4 cut(s) 1000, 1274, 3193, 3667
TseI GCWGC 9 cut(s) 16, 101, 1823, 2129, 2521, 2588, 2591, 3160, 3780
Tsp45I GTSAC 4 cut(s) 1000, 1274, 3193, 3667
TspGWI ACGGA 1 cut(s) 1997
Van91I CCANNNNNTGG 1 cut(s) 1354
Vha464I CTTAAG 1 cut(s) 1939
VpaK11BI GGWCC 4 cut(s) 1344, 1984, 3008, 3773
VspI ATTAAT 3 cut(s) 1992, 2457, 3735
XapI RAATTY 9 cut(s) 734, 759, 802, 1643, 1736, 1768, 2831, 3243, 3833
XbaI TCTAGA 1 cut(s) 1843
XceI RCATGY 7 cut(s) 131, 651, 1832, 2741, 2763, 3233, 3918
XcmI CCANNNNNNNNNTGG 2 cut(s) 2276, 3703
XmiI GTMKAC 1 cut(s) 3138
XmnI GAANNNNTTC 3 cut(s) 249, 1290, 1789
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 3311
Zsp2I ATGCAT 2 cut(s) 2359, 2761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.