AT2G01620

MEE11 MEE11 (maternal effect embryo arrest 11)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015893
Physical Location & Seq
Forward (+)
278124 .. 279296
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01620.1

Sequence Viewer

Length: 879 bp
ATGAAGACGGAGCTTGAAGAAGAAGAAGAAGAAGAATGGAGATCCGTTCACGAGGTTTTATTAATCGTTCTTCCTTATCTTCATTCTCTGTTCGAGCTTCTCTCTATGATTCGCGTTTCGCGATCTCTCAGAGACGCGATCAGAGACGAAACCGCGTTATGGACAAAACTCGTTATTGAACCACCGTTGAGCTCTCGTCTAACCGATGATATTCTATCGGAGTTCAGTTCCAAATCCGCCGGAAAGCTGAAAACTCTGATTCTCCGGCAATGTCTTATGGTCACCAACAAGGGACTCCGTCGTGTTGTGGATGCTAATCCTCTCATCACTAAGATAATTGTGCCTGGATGCTCTGGATTGACTCCTGAAGGAATCATGGAATGTGTTGAAAGCTTGTCTAAGAACAATCATAAACTAGAGACACTTCACATCAATGGCGTCAATGGCTTTACCAAACAACATCTCTCAGCACTGTATACTTACCTTTCTTCCGAGGGAACGATTGACCTCGAGGTCTGTCCCAAATGCGATGAAGTAAGGATGATTCCGTCTTGCTCAAGAGAATCATGTAACCAGAAGCAACGCAAGTGCCGTGGATGCTGGCTTTGTATCCCTAGATGTGCAGAGTGCGCAGTGTGCCTTGTTGGTTCAGATACTGAGAGTCAAGAAGCAGCGTGTGGTAATGATGATGTTCTGTGTCTTGAATGTTGGCTTGTGCTTCCCAAGTGCAGATTCTGCAATAAACCATATTGCACTAATCACAGTAGTCGTCGACATGAGATTGCAATCACTGATGCTGCATCTCGGCCTTCGTTTGAATGTGAAGCTTGTTACTATAGGGCAGGTACAAACCCATATGAAGTCGACTATCAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

292

Amino Acids

33.0

Weight (kDa)

5.62

Isoelectric Point (pI)

60.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 17 - 59 4.2e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01620
fragaria_vesca FvH4_4g33880
malus_domestica MD13G1030300.v1.1
prunus_persica Prupe.1G331200_v2.0.a1
pyrus_communis pycom13g01860
rosa_chinensis RchiOBHm_Chr4g0443391
rosa_laevigata RLG00000005932
rosa_multiflora Rmu_ssc0000122.1_g000014
rosa_roxburghii Rroxscaffold_5G00383950
rosa_rugosa Rorug04G0346000
rosa_samantha Rh4AG401300 Rh4BG412700 Rh4CG428400 Rh4DG408500
rosa_wichuraiana Rw4G034600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 512
AbsI CCTCGAGG 1 cut(s) 509
Acc16I TGCGCA 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 833
AccI GTMKAC 3 cut(s) 476, 772, 864
AccII CGCG 4 cut(s) 114, 121, 137, 155
AciI CCGC 2 cut(s) 153, 237
AclWI GGATC 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 1 cut(s) 847
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 5 cut(s) 17, 179, 389, 704, 818
AjnI CCWGG 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AluBI AGCT 6 cut(s) 13, 97, 192, 247, 393, 827
AluI AGCT 6 cut(s) 13, 97, 192, 247, 393, 827
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 3 cut(s) 126, 138, 413
AlwI GGATC 1 cut(s) 36
AlwNI CAGNNNCTG 2 cut(s) 656, 735
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 1 cut(s) 806
ApeKI GCWGC 2 cut(s) 671, 797
AseI ATTAAT 1 cut(s) 62
AspLEI GCGC 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 509
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 683, 784
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 620
BcoDI GTCTC 3 cut(s) 126, 138, 413
BfaI CTAG 2 cut(s) 416, 615
BfmI CTRYAG 1 cut(s) 835
BfuAI ACCTGC 1 cut(s) 833
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 2 cut(s) 672, 798
BlsI GCNGC 2 cut(s) 673, 799
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 5 cut(s) 301, 338, 587, 784, 809
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 2 cut(s) 315, 785
BsaHI GRCGYC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 492, 592
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 2 cut(s) 315, 785
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 492, 592
BseGI GGATG 4 cut(s) 316, 353, 546, 602
BseJI GATNNNNATC 2 cut(s) 315, 785
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 3 cut(s) 142, 480, 648
BseXI GCAGC 2 cut(s) 683, 784
BsgI GTGCAG 2 cut(s) 642, 748
Bsh1236I CGCG 4 cut(s) 114, 121, 137, 155
BshFI GGCC 1 cut(s) 808
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 240, 265
BslFI GGGAC 2 cut(s) 306, 504
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 3 cut(s) 126, 138, 413
BsmBI CGTCTC 2 cut(s) 126, 138
BsmFI GGGAC 2 cut(s) 306, 504
BsnI GGCC 1 cut(s) 808
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 3 cut(s) 41, 122, 138
Bsp68I TCGCGA 1 cut(s) 121
BspACI CCGC 2 cut(s) 153, 237
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 3 cut(s) 141, 479, 649
BspFNI CGCG 4 cut(s) 114, 121, 137, 155
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 492, 592
BssMI GATC 3 cut(s) 41, 122, 138
BssNAI GTATAC 1 cut(s) 477
BssNI GRCGYC 1 cut(s) 438
BssSI CACGAG 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 477
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 186, 474, 764
BstACI GRCGYC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 735
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 5 cut(s) 128, 330, 399, 466, 657
BstDSI CCRYGG 1 cut(s) 592
BstEII GGTNACC 1 cut(s) 280
BstF5I GGATG 4 cut(s) 316, 353, 546, 602
BstFNI CGCG 4 cut(s) 114, 121, 137, 155
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 3 cut(s) 44, 125, 141
BstMAI GTCTC 3 cut(s) 126, 138, 413
BstMBI GATC 3 cut(s) 41, 122, 138
BstMWI GCNNNNNNNGC 5 cut(s) 444, 597, 629, 636, 735
BstNI CCWGG 1 cut(s) 345
BstPI GGTNACC 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 835
BstUI CGCG 4 cut(s) 114, 121, 137, 155
BstV1I GCAGC 2 cut(s) 683, 784
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BstZ17I GTATAC 1 cut(s) 477
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 808
BtgI CCRYGG 1 cut(s) 592
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 4 cut(s) 316, 353, 546, 602
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 3 cut(s) 470, 639, 789
BtuMI TCGCGA 1 cut(s) 121
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 2 cut(s) 656, 735
CfoI GCGC 1 cut(s) 632
CseI GACGC 2 cut(s) 143, 427
Csp6I GTAC 1 cut(s) 846
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 3 cut(s) 376, 567, 776
CviQI GTAC 1 cut(s) 846
DdeI CTNAG 5 cut(s) 128, 330, 399, 466, 657
DpnI GATC 3 cut(s) 43, 124, 140
DpnII GATC 3 cut(s) 41, 122, 138
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 194
Esp3I CGTCTC 2 cut(s) 126, 138
FaeI CATG 3 cut(s) 379, 570, 779
FaqI GGGAC 2 cut(s) 306, 504
FatI CATG 3 cut(s) 375, 566, 775
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 3 cut(s) 476, 772, 864
Fnu4HI GCNGC 2 cut(s) 672, 798
FokI GGATG 4 cut(s) 323, 360, 553, 609
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 672, 798
FspBI CTAG 2 cut(s) 416, 615
FspI TGCGCA 1 cut(s) 631
GlaI GCGC 1 cut(s) 631
GluI GCNGC 2 cut(s) 672, 798
HaeIII GGCC 1 cut(s) 808
HapII CCGG 2 cut(s) 240, 265
HgaI GACGC 2 cut(s) 143, 427
HhaI GCGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 3 cut(s) 379, 570, 779
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 2 cut(s) 773, 865
HindII GTYRAC 2 cut(s) 773, 865
HindIII AAGCTT 2 cut(s) 391, 825
HinfI GANTC 9 cut(s) 109, 259, 294, 361, 372, 544, 563, 661, 732
HpaII CCGG 2 cut(s) 240, 265
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 4 cut(s) 49, 477, 773, 865
Hpy188I TCNGA 6 cut(s) 131, 143, 220, 258, 493, 652
Hpy188III TCNNGA 7 cut(s) 50, 120, 354, 365, 558, 665, 701
Hpy8I GTNNAC 4 cut(s) 49, 477, 773, 865
Hpy99I CGWCG 2 cut(s) 303, 774
HpyAV CCTTC 2 cut(s) 362, 819
HpyCH4III ACNGT 3 cut(s) 186, 474, 764
HpyCH4V TGCA 6 cut(s) 623, 729, 738, 753, 785, 800
HpyF10VI GCNNNNNNNGC 5 cut(s) 444, 597, 629, 636, 735
HpyF3I CTNAG 5 cut(s) 128, 330, 399, 466, 657
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 3 cut(s) 379, 570, 779
HspAI GCGC 1 cut(s) 630
Kzo9I GATC 3 cut(s) 41, 122, 138
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 9 cut(s) 253, 278, 330, 339, 357, 378, 586, 587, 828
Lsp1109I GCAGC 2 cut(s) 683, 784
LweI GCATC 5 cut(s) 301, 338, 587, 784, 809
MaeI CTAG 2 cut(s) 416, 615
MaeIII GTNAC 3 cut(s) 280, 569, 830
MalI GATC 3 cut(s) 43, 124, 140
MboI GATC 3 cut(s) 41, 122, 138
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 1 cut(s) 336
MlyI GAGTC 3 cut(s) 288, 355, 670
MnlI CCTC 5 cut(s) 46, 330, 487, 505, 518
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 2 cut(s) 240, 265
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 4 cut(s) 114, 121, 137, 155
MwoI GCNNNNNNNGC 5 cut(s) 444, 597, 629, 636, 735
NdeI CATATG 1 cut(s) 856
NdeII GATC 3 cut(s) 41, 122, 138
NlaIII CATG 3 cut(s) 379, 570, 779
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 1 cut(s) 280
NruI TCGCGA 1 cut(s) 121
NsbI TGCGCA 1 cut(s) 631
PaeR7I CTCGAG 1 cut(s) 509
PcsI WCGNNNNNNNCGW 2 cut(s) 118, 589
PfeI GAWTC 6 cut(s) 109, 259, 372, 544, 563, 732
PflFI GACNNNGTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 673, 799
PleI GAGTC 3 cut(s) 288, 355, 669
PpsI GAGTC 3 cut(s) 288, 355, 669
PshBI ATTAAT 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 343
PspEI GGTNACC 1 cut(s) 280
PspGI CCWGG 1 cut(s) 343
PspXI VCTCGAGB 1 cut(s) 509
PstNI CAGNNNCTG 2 cut(s) 656, 735
PsuI RGATCY 1 cut(s) 41
PsyI GACNNNGTC 1 cut(s) 297
RruI TCGCGA 1 cut(s) 121
RsaI GTAC 1 cut(s) 847
RsaNI GTAC 1 cut(s) 846
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 194
SalI GTCGAC 2 cut(s) 771, 863
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 2 cut(s) 672, 798
Sau3AI GATC 3 cut(s) 41, 122, 138
SchI GAGTC 3 cut(s) 288, 355, 670
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 5 cut(s) 301, 338, 587, 784, 809
SfcI CTRYAG 1 cut(s) 835
Sfr274I CTCGAG 1 cut(s) 509
SlaI CTCGAG 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 509, 556
SmoI CTYRAG 2 cut(s) 509, 556
Sse9I AATT 1 cut(s) 336
SsiI CCGC 2 cut(s) 153, 237
SspMI CTAG 2 cut(s) 416, 615
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 3 cut(s) 186, 474, 764
TaqI TCGA 4 cut(s) 93, 510, 772, 864
TasI AATT 1 cut(s) 336
TfiI GAWTC 6 cut(s) 109, 259, 372, 544, 563, 732
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 3 cut(s) 477, 639, 796
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 671, 797
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 4 cut(s) 17, 71, 546, 873
TspGWI ACGGA 4 cut(s) 23, 34, 287, 537
TspRI CASTG 3 cut(s) 477, 639, 796
Tth111I GACNNNGTC 1 cut(s) 297
VspI ATTAAT 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 509
XmiI GTMKAC 3 cut(s) 476, 772, 864
XspI CTAG 2 cut(s) 416, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.