pycom13g01860

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015893
Physical Location & Seq
Reverse (-)
1253886 .. 1255021
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01860.2

Sequence Viewer

Length: 978 bp
ATGGAGATTTCAGAGTCTACGGTGGAACACCAAGAACATGAAGCTACTGACAGTTCGGGAGATGAAAAAGCAGGGCCACCGCACGAGGCATTGTTCCTTGTTTCAGCTTACCTTCCTCTGTTCGAGCTTCTTGCTGTTAGCGGAGTTTGTAGGATACTGAGGGATGCAGTAAACACAGATGTATTACCATGGCTAAATATCATCGTCAAAGCACCGTTGAACTTGAGGATGTCTGATGAGGTTTTGAAGAAAATCACATCCAAGGCTAATGGTAGGTTGAGATCTCTGGCTTTGATGAACTGTGCCAATGTTACAGATGAGGGGCTTCAAAGGGTCGTTGAGCAGAATCCTCTTATCAACAAGCTCTACGTACCGGGATGCACCGGTTTAACACCCAACGGAGTCATTCGAGCTGCCAAAACATTATCCGAAAATCATCACGGCTTAAAGAGCGTAATGATATACGGCATCTACAACATGAACAAACAACACCTCGAAACGCTTGAGTCATCTCTCCAGATAAACCGGTCACAGCAAAAGCAGCCAGGAGGATCACGGCCTCTTTTGTTTCACGAGTACAAGGACTGCCCAACCTCCAGACACGACAATGGTCACGCTGCAATCGACGTCCAAGTTTGCCCAAAATGCGACGAGGTTAGGATGGTTTTCGACTGTCCAAGGAGGACGTGCAGGACAAAGATAGACAGGCCAATGACTGATTGCAGAGGATGCAACTTTTGCATTCCGAGGTGCCAAGAGTGCGGCGGATGCGTCGACTGTGAAGAAATGGAGGAGGCTGTTTGTGCAGATATCTTGTGTTCGGATTGCTGGCTGCAACTTCCCAAATGTGACTTCTGCAACAAGCCGTACTGTAAGCAACACGCCGGCAAAGGATACTGTCCTCCTGGTTCCACCGGGTTCGTTTGCGATGTTTGTTATACAAAATTCATTGTAGATTTGCACAGCGTTGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

326

Amino Acids

36.2

Weight (kDa)

6.01

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01620
fragaria_vesca FvH4_4g33880
malus_domestica MD13G1030300.v1.1
prunus_persica Prupe.1G331200_v2.0.a1
pyrus_communis pycom13g01860
rosa_chinensis RchiOBHm_Chr4g0443391
rosa_laevigata RLG00000005932
rosa_multiflora Rmu_ssc0000122.1_g000014
rosa_roxburghii Rroxscaffold_5G00383950
rosa_rugosa Rorug04G0346000
rosa_samantha Rh4AG401300 Rh4BG412700 Rh4CG428400 Rh4DG408500
rosa_wichuraiana Rw4G034600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 630
AccB1I GGYRCC 1 cut(s) 750
AccI GTMKAC 2 cut(s) 17, 774
AciI CCGC 4 cut(s) 80, 141, 762, 765
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 944
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 3 cut(s) 372, 578, 869
AgeI ACCGGT 2 cut(s) 383, 525
AgsI TTSAA 4 cut(s) 220, 247, 329, 974
AjiI CACGTC 1 cut(s) 687
AjnI CCWGG 2 cut(s) 544, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
AluBI AGCT 5 cut(s) 44, 107, 127, 364, 413
AluI AGCT 5 cut(s) 44, 107, 127, 364, 413
AlwI GGATC 1 cut(s) 559
AoxI GGCC 3 cut(s) 74, 557, 707
ApeKI GCWGC 4 cut(s) 413, 541, 617, 832
ApoI RAATTY 1 cut(s) 944
AsiGI ACCGGT 2 cut(s) 383, 525
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 2 cut(s) 375, 916
BanI GGYRCC 1 cut(s) 750
BauI CACGAG 2 cut(s) 83, 572
BbvI GCAGC 4 cut(s) 400, 553, 604, 819
BccI CCATC 1 cut(s) 655
BceAI ACGGC 4 cut(s) 457, 481, 572, 850
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 546, 906
BciVI GTATCC 2 cut(s) 147, 887
BcnI CCSGG 2 cut(s) 375, 916
BfuI GTATCC 2 cut(s) 147, 887
BglII AGATCT 1 cut(s) 281
BisI GCNGC 5 cut(s) 414, 542, 618, 763, 833
BlsI GCNGC 5 cut(s) 415, 543, 619, 764, 834
Bme1390I CCNGG 4 cut(s) 375, 546, 906, 916
BmgBI CACGTC 1 cut(s) 687
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 752, 910
BmrFI CCNGG 4 cut(s) 375, 546, 906, 916
BmsI GCATC 5 cut(s) 154, 368, 477, 719, 758
BoxI GACNNNNGTC 1 cut(s) 609
BpmI CTGGAG 2 cut(s) 500, 580
BpuEI CTTGAG 2 cut(s) 244, 524
BpuMI CCSGG 2 cut(s) 375, 916
BsaAI YACGTR 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 627
BsaJI CCNNGG 4 cut(s) 188, 261, 677, 746
BsaWI WCCGGW 2 cut(s) 383, 525
Bse118I RCCGGY 3 cut(s) 383, 525, 884
BseBI CCWGG 2 cut(s) 546, 906
BseDI CCNNGG 4 cut(s) 188, 261, 677, 746
BseGI GGATG 7 cut(s) 169, 234, 257, 383, 666, 734, 773
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 806
BseXI GCAGC 4 cut(s) 400, 553, 604, 819
BsgI GTGCAG 2 cut(s) 709, 825
BshFI GGCC 3 cut(s) 76, 559, 709
BshNI GGYRCC 1 cut(s) 750
BshTI ACCGGT 2 cut(s) 383, 525
BsiSI CCGG 5 cut(s) 374, 384, 526, 885, 915
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 3 cut(s) 76, 559, 709
Bsp143I GATC 2 cut(s) 281, 551
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 4 cut(s) 80, 141, 762, 765
BspANI GGCC 3 cut(s) 76, 559, 709
BspCNI CTCAG 1 cut(s) 150
BspLI GGNNCC 2 cut(s) 752, 910
BspPI GGATC 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 750
BsrFI RCCGGY 3 cut(s) 383, 525, 884
BssAI RCCGGY 3 cut(s) 383, 525, 884
BssECI CCNNGG 4 cut(s) 188, 261, 677, 746
BssMI GATC 2 cut(s) 281, 551
BssNI GRCGYC 1 cut(s) 627
BssSI CACGAG 2 cut(s) 83, 572
BssT1I CCWWGG 3 cut(s) 188, 261, 677
Bst2BI CACGAG 2 cut(s) 83, 572
Bst2UI CCWGG 2 cut(s) 546, 906
Bst4CI ACNGT 8 cut(s) 22, 53, 216, 302, 674, 779, 872, 899
BstACI GRCGYC 1 cut(s) 627
BstAPI GCANNNNNTGC 2 cut(s) 729, 738
BstBAI YACGTR 1 cut(s) 370
BstC8I GCNNGC 2 cut(s) 830, 886
BstDEI CTNAG 1 cut(s) 158
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 7 cut(s) 169, 234, 257, 383, 666, 734, 773
BstKTI GATC 2 cut(s) 284, 554
BstMBI GATC 2 cut(s) 281, 551
BstMWI GCNNNNNNNGC 8 cut(s) 450, 541, 645, 729, 738, 759, 768, 803
BstNI CCWGG 2 cut(s) 546, 906
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 4 cut(s) 373, 544, 904, 914
BstSNI TACGTA 1 cut(s) 370
BstV1I GCAGC 4 cut(s) 400, 553, 604, 819
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
BsuI GTATCC 2 cut(s) 147, 887
BsuRI GGCC 3 cut(s) 76, 559, 709
BtgI CCRYGG 1 cut(s) 188
BtgZI GCGATG 1 cut(s) 942
BtrI CACGTC 1 cut(s) 687
BtsCI GGATG 7 cut(s) 169, 234, 257, 383, 666, 734, 773
Cac8I GCNNGC 2 cut(s) 830, 886
Cfr10I RCCGGY 3 cut(s) 383, 525, 884
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 1 cut(s) 760
Csp6I GTAC 3 cut(s) 371, 577, 868
CspAI ACCGGT 2 cut(s) 383, 525
CviAII CATG 3 cut(s) 38, 189, 478
CviQI GTAC 3 cut(s) 371, 577, 868
DdeI CTNAG 1 cut(s) 158
DpnI GATC 2 cut(s) 283, 553
DpnII GATC 2 cut(s) 281, 551
EciI GGCGGA 1 cut(s) 780
Eco105I TACGTA 1 cut(s) 370
Eco130I CCWWGG 3 cut(s) 188, 261, 677
Eco32I GATATC 1 cut(s) 811
EcoRII CCWGG 2 cut(s) 544, 904
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 3 cut(s) 188, 261, 677
ErhI CCWWGG 3 cut(s) 188, 261, 677
FaeI CATG 3 cut(s) 41, 192, 481
FaiI YATR 5 cut(s) 39, 190, 463, 479, 939
FatI CATG 3 cut(s) 37, 188, 477
FblI GTMKAC 2 cut(s) 17, 774
Fnu4HI GCNGC 5 cut(s) 414, 542, 618, 763, 833
FokI GGATG 7 cut(s) 176, 241, 244, 390, 673, 741, 780
Fsp4HI GCNGC 5 cut(s) 414, 542, 618, 763, 833
GluI GCNGC 5 cut(s) 414, 542, 618, 763, 833
GsuI CTGGAG 2 cut(s) 500, 580
HaeIII GGCC 3 cut(s) 76, 559, 709
HapII CCGG 5 cut(s) 374, 384, 526, 885, 915
HgaI GACGC 1 cut(s) 760
Hin1I GRCGYC 1 cut(s) 627
Hin1II CATG 3 cut(s) 41, 192, 481
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 4 cut(s) 14, 346, 402, 506
HpaII CCGG 5 cut(s) 374, 384, 526, 885, 915
Hpy166II GTNNAC 3 cut(s) 18, 172, 775
Hpy188I TCNGA 5 cut(s) 13, 235, 430, 747, 823
Hpy188III TCNNGA 4 cut(s) 57, 517, 572, 597
Hpy8I GTNNAC 3 cut(s) 18, 172, 775
Hpy99I CGWCG 3 cut(s) 629, 653, 776
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 8 cut(s) 22, 53, 216, 302, 674, 779, 872, 899
HpyCH4IV ACGT 3 cut(s) 369, 627, 686
HpyF10VI GCNNNNNNNGC 8 cut(s) 450, 541, 645, 729, 738, 759, 768, 803
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 3 cut(s) 369, 627, 686
Hsp92I GRCGYC 1 cut(s) 627
Hsp92II CATG 3 cut(s) 41, 192, 481
KroI GCCGGC 1 cut(s) 884
KroNI GCCGGC 1 cut(s) 886
Kzo9I GATC 2 cut(s) 281, 551
Lsp1109I GCAGC 4 cut(s) 400, 553, 604, 819
LweI GCATC 5 cut(s) 154, 368, 477, 719, 758
MaeII ACGT 3 cut(s) 369, 627, 686
MaeIII GTNAC 4 cut(s) 310, 528, 611, 848
MalI GATC 2 cut(s) 283, 553
MboI GATC 2 cut(s) 281, 551
MboII GAAGA 2 cut(s) 259, 794
MflI RGATCY 1 cut(s) 281
MluCI AATT 1 cut(s) 944
MlyI GAGTC 3 cut(s) 23, 411, 515
MroNI GCCGGC 1 cut(s) 884
MseI TTAA 2 cut(s) 389, 446
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 5 cut(s) 374, 384, 526, 885, 915
MspR9I CCNGG 4 cut(s) 375, 546, 906, 916
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 2 cut(s) 546, 906
MwoI GCNNNNNNNGC 8 cut(s) 450, 541, 645, 729, 738, 759, 768, 803
NaeI GCCGGC 1 cut(s) 886
NciI CCSGG 2 cut(s) 375, 916
NcoI CCATGG 1 cut(s) 188
NdeII GATC 2 cut(s) 281, 551
NgoMIV GCCGGC 1 cut(s) 884
NlaIII CATG 3 cut(s) 41, 192, 481
NlaIV GGNNCC 2 cut(s) 752, 910
NmuCI GTSAC 3 cut(s) 528, 611, 848
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PctI GAATGC 1 cut(s) 741
PdiI GCCGGC 1 cut(s) 886
PfeI GAWTC 1 cut(s) 346
PinAI ACCGGT 2 cut(s) 383, 525
PkrI GCNGC 5 cut(s) 415, 543, 619, 764, 834
PleI GAGTC 3 cut(s) 22, 410, 514
PpsI GAGTC 3 cut(s) 22, 410, 514
Ppu21I YACGTR 1 cut(s) 370
PshAI GACNNNNGTC 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 544, 904
PspGI CCWGG 2 cut(s) 544, 904
PspN4I GGNNCC 2 cut(s) 752, 910
PspPI GGNCC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 3 cut(s) 372, 578, 869
RsaNI GTAC 3 cut(s) 371, 577, 868
RseI CAYNNNNRTG 1 cut(s) 606
SalI GTCGAC 1 cut(s) 773
SaqAI TTAA 2 cut(s) 389, 446
SatI GCNGC 5 cut(s) 414, 542, 618, 763, 833
Sau3AI GATC 2 cut(s) 281, 551
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 3 cut(s) 23, 411, 515
ScrFI CCNGG 4 cut(s) 375, 546, 906, 916
SfaNI GCATC 5 cut(s) 154, 368, 477, 719, 758
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 2 cut(s) 223, 503
SmoI CTYRAG 2 cut(s) 223, 503
SnaBI TACGTA 1 cut(s) 370
Sse9I AATT 1 cut(s) 944
SsiI CCGC 4 cut(s) 80, 141, 762, 765
StyD4I CCNGG 4 cut(s) 373, 544, 904, 914
StyI CCWWGG 3 cut(s) 188, 261, 677
TaaI ACNGT 8 cut(s) 22, 53, 216, 302, 674, 779, 872, 899
TaiI ACGT 3 cut(s) 372, 630, 689
TaqI TCGA 6 cut(s) 123, 409, 495, 624, 669, 774
TasI AATT 1 cut(s) 944
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 765
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 2 cut(s) 389, 446
Tru9I TTAA 2 cut(s) 389, 446
TseFI GTSAC 3 cut(s) 528, 611, 848
TseI GCWGC 4 cut(s) 413, 541, 617, 832
Tsp45I GTSAC 3 cut(s) 528, 611, 848
TspDTI ATGAA 5 cut(s) 54, 78, 311, 494, 937
TspGWI ACGGA 1 cut(s) 414
XapI RAATTY 1 cut(s) 944
XmiI GTMKAC 2 cut(s) 17, 774
ZraI GACGTC 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.