Rroxscaffold_5G00383950

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015893
Physical Location & Seq
Reverse (-)
63513074 .. 63517594
4521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383950.1

Sequence Viewer

Length: 1026 bp
ATGGAGATCCCACAGATTCTGGAGTACCAAGAACATGAAGCTGTTGACAACTCTGCACAGAAGGTACCAATTCTGCCTTCTGATGCAATCAAAGAAAAAGAAGCAGGGCCACCTCACGAAGCTTTGTTCCTTATCTTAACTTACCTTCCTCTGTTTGAGCTTCTTGCCATGAATCAAGTTTGCACTTCACTGAGAGATGCAGTAAACAAGGATGTGCTCTCATGGTTGAACATCATCGTTGATAAGGCCTTGAGCTTGAAATTGTCTGATGAGATCTTGATGAAAATTACATCGAAGGCTAATGGTAGGTTGAGAACTTTGGCTCTTATGAACTGTGCAAAGATTACAGATAACGGGCTTCAACAGGTTGTGGAGCAGAATCCTCTGATCAGCAAGCTCTATCTACCAGCTTGCACTGGCTTAACACCCGAAGGGATCATACGAGCTGTGAAGACATTATCTGACCATGGTCACTGCCTAAGGTCCATAATGATAAATGGCATCTACAACATAAACAAACAACACCTTGAAACACTTGAGTCTTATCTTCATGAAATGAATCTCGCCGCAAAGAAAGAGCAGCCTCGATCATGGCCTCTCCTACTACTTGAGTACATGGACTCGCCAACATTCAGACATGACAAAGGTCACACCATAATTGACCTTGAAGTTTGCCCGAAATGTGACGAGGTGAGGATGGTTTTCGACTGTCCAAGGTGGGCGTGCAAGAGAATGATAGAAAGGTCAATGACTCGGTGTAGGGGATGCAAGTTTTGCATTCTGAGGTGCCAAGAGTGTGGTGGATGCGTCGATTCTCAAGAAATTGAAGAGGCTGTTTGTGCTCATATCTTGTGCTCAGATTGCTGGCTCCACCTTCCCAAATGTAACTTCTGCAACAAGCCATATTGCAAGCAACACGCTGATAACAAGTGCTGCACTTCAGGATCCACAGGTTTTGTCTGTGAGGTTTGTTATGCAAAGTACGTTGTAAACTTGCCCAGTATTGTTGAATACGTTGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

341

Amino Acids

38.66

Weight (kDa)

6.14

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 38 - 75 5.6e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01620
fragaria_vesca FvH4_4g33880
malus_domestica MD13G1030300.v1.1
prunus_persica Prupe.1G331200_v2.0.a1
pyrus_communis pycom13g01860
rosa_chinensis RchiOBHm_Chr4g0443391
rosa_laevigata RLG00000005932
rosa_multiflora Rmu_ssc0000122.1_g000014
rosa_roxburghii Rroxscaffold_5G00383950
rosa_rugosa Rorug04G0346000
rosa_samantha Rh4AG401300 Rh4BG412700 Rh4CG428400 Rh4DG408500
rosa_wichuraiana Rw4G034600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 64
AccB1I GGYRCC 2 cut(s) 64, 786
AciI CCGC 1 cut(s) 567
AclWI GGATC 3 cut(s) 443, 939, 952
AcuI CTGAAG 1 cut(s) 924
AfaI GTAC 4 cut(s) 26, 66, 614, 983
AgsI TTSAA 7 cut(s) 229, 259, 362, 530, 668, 827, 1010
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 872, 904
AluBI AGCT 7 cut(s) 41, 122, 160, 255, 397, 410, 446
AluI AGCT 7 cut(s) 41, 122, 160, 255, 397, 410, 446
Alw21I GWGCWC 3 cut(s) 219, 844, 857
AlwI GGATC 3 cut(s) 443, 939, 952
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 3 cut(s) 107, 246, 593
ApeKI GCWGC 2 cut(s) 580, 933
Asp718I GGTACC 1 cut(s) 64
AspS9I GGNCC 2 cut(s) 107, 483
AsuHPI GGTGA 1 cut(s) 703
AvaII GGWCC 1 cut(s) 483
AxyI CCTNAGG 1 cut(s) 479
BamHI GGATCC 1 cut(s) 944
BanI GGYRCC 2 cut(s) 64, 786
BarI GAAGNNNNNNTAC 2 cut(s) 423, 455
BbsI GAAGAC 1 cut(s) 458
Bbv12I GWGCWC 3 cut(s) 219, 844, 857
BbvI GCAGC 2 cut(s) 592, 920
BccI CCATC 1 cut(s) 691
BclI TGATCA 1 cut(s) 387
BglII AGATCT 1 cut(s) 273
BisI GCNGC 3 cut(s) 567, 581, 934
BlsI GCNGC 3 cut(s) 568, 582, 935
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 2 cut(s) 107, 483
BmiI GGNNCC 4 cut(s) 66, 788, 869, 946
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 5 cut(s) 73, 187, 510, 755, 794
BmuI ACTGGG 1 cut(s) 993
BoxI GACNNNNGTC 2 cut(s) 468, 645
BpiI GAAGAC 1 cut(s) 458
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 4 cut(s) 271, 557, 629, 801
BsaJI CCNNGG 2 cut(s) 466, 713
Bse1I ACTGG 2 cut(s) 421, 999
Bse21I CCTNAGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 466, 713
BseGI GGATG 4 cut(s) 217, 702, 770, 809
BseMII CTCAG 3 cut(s) 182, 773, 870
BseNI ACTGG 2 cut(s) 421, 999
BseXI GCAGC 2 cut(s) 592, 920
BsgI GTGCAG 2 cut(s) 39, 919
BshFI GGCC 3 cut(s) 109, 248, 595
BshNI GGYRCC 2 cut(s) 64, 786
BsiHKAI GWGCWC 3 cut(s) 219, 844, 857
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 109, 248, 595
Bsp1286I GDGCHC 3 cut(s) 219, 844, 857
Bsp143I GATC 6 cut(s) 6, 273, 387, 435, 587, 944
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 1 cut(s) 567
BspANI GGCC 3 cut(s) 109, 248, 595
BspCNI CTCAG 3 cut(s) 183, 774, 869
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 4 cut(s) 66, 788, 869, 946
BspPI GGATC 3 cut(s) 443, 939, 952
BspT107I GGYRCC 2 cut(s) 64, 786
BsrI ACTGG 2 cut(s) 421, 999
BssECI CCNNGG 2 cut(s) 466, 713
BssMI GATC 6 cut(s) 6, 273, 387, 435, 587, 944
BssT1I CCWWGG 2 cut(s) 466, 713
Bst4CI ACNGT 2 cut(s) 335, 710
Bst6I CTCTTC 1 cut(s) 822
BstAPI GCANNNNNTGC 1 cut(s) 774
BstC8I GCNNGC 5 cut(s) 395, 412, 724, 866, 911
BstDEI CTNAG 4 cut(s) 191, 479, 782, 856
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 4 cut(s) 217, 702, 770, 809
BstKTI GATC 6 cut(s) 9, 276, 390, 438, 590, 947
BstMBI GATC 6 cut(s) 6, 273, 387, 435, 587, 944
BstMWI GCNNNNNNNGC 3 cut(s) 774, 839, 861
BstPAI GACNNNNGTC 2 cut(s) 468, 645
BstV1I GCAGC 2 cut(s) 592, 920
BstV2I GAAGAC 1 cut(s) 458
BstX2I RGATCY 3 cut(s) 6, 273, 944
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 3 cut(s) 6, 273, 944
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 109, 248, 595
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 4 cut(s) 217, 702, 770, 809
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 3 cut(s) 188, 414, 472
Cac8I GCNNGC 5 cut(s) 395, 412, 724, 866, 911
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 107, 483
CseI GACGC 1 cut(s) 796
Csp6I GTAC 4 cut(s) 25, 65, 613, 982
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviAII CATG 8 cut(s) 35, 169, 222, 467, 551, 591, 616, 638
CviQI GTAC 4 cut(s) 25, 65, 613, 982
DdeI CTNAG 4 cut(s) 191, 479, 782, 856
DpnI GATC 6 cut(s) 8, 275, 389, 437, 589, 946
DpnII GATC 6 cut(s) 6, 273, 387, 435, 587, 944
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Eco130I CCWWGG 2 cut(s) 466, 713
Eco147I AGGCCT 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 924
Eco81I CCTNAGG 1 cut(s) 479
EcoT14I CCWWGG 2 cut(s) 466, 713
ErhI CCWWGG 2 cut(s) 466, 713
FaeI CATG 8 cut(s) 38, 172, 225, 470, 554, 594, 619, 641
FatI CATG 8 cut(s) 34, 168, 221, 466, 550, 590, 615, 637
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 567, 581, 934
FokI GGATG 4 cut(s) 224, 709, 777, 816
Fsp4HI GCNGC 3 cut(s) 567, 581, 934
GluI GCNGC 3 cut(s) 567, 581, 934
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 3 cut(s) 109, 248, 595
HgaI GACGC 1 cut(s) 796
Hin1II CATG 8 cut(s) 38, 172, 225, 470, 554, 594, 619, 641
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 8 cut(s) 16, 172, 379, 539, 559, 620, 751, 812
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 3 cut(s) 46, 205, 991
Hpy188I TCNGA 8 cut(s) 82, 268, 387, 463, 635, 783, 859, 1025
Hpy188III TCNNGA 6 cut(s) 20, 116, 277, 551, 818, 942
Hpy8I GTNNAC 3 cut(s) 46, 205, 991
Hpy99I CGWCG 1 cut(s) 812
HpyAV CCTTC 6 cut(s) 55, 87, 155, 289, 425, 884
HpyCH4III ACNGT 2 cut(s) 335, 710
HpyCH4IV ACGT 2 cut(s) 984, 1014
HpyF10VI GCNNNNNNNGC 3 cut(s) 774, 839, 861
HpyF3I CTNAG 4 cut(s) 191, 479, 782, 856
HpySE526I ACGT 2 cut(s) 984, 1014
Hsp92II CATG 8 cut(s) 38, 172, 225, 470, 554, 594, 619, 641
KpnI GGTACC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 6 cut(s) 6, 273, 387, 435, 587, 944
LmnI GCTCC 2 cut(s) 373, 873
LpnPI CCDG 9 cut(s) 5, 90, 350, 402, 420, 850, 927, 936, 1012
Lsp1109I GCAGC 2 cut(s) 592, 920
LweI GCATC 5 cut(s) 73, 187, 510, 755, 794
MaeII ACGT 2 cut(s) 984, 1014
MaeIII GTNAC 4 cut(s) 470, 647, 683, 884
MalI GATC 6 cut(s) 8, 275, 389, 437, 589, 946
MboI GATC 6 cut(s) 6, 273, 387, 435, 587, 944
MboII GAAGA 3 cut(s) 463, 539, 839
MflI RGATCY 3 cut(s) 6, 273, 944
MhlI GDGCHC 3 cut(s) 219, 844, 857
MluCI AATT 5 cut(s) 69, 260, 285, 657, 822
MlyI GAGTC 3 cut(s) 548, 614, 745
MseI TTAA 2 cut(s) 137, 422
Mva1269I GAATGC 1 cut(s) 777
MwoI GCNNNNNNNGC 3 cut(s) 774, 839, 861
NcoI CCATGG 1 cut(s) 466
NdeII GATC 6 cut(s) 6, 273, 387, 435, 587, 944
NlaIII CATG 8 cut(s) 38, 172, 225, 470, 554, 594, 619, 641
NlaIV GGNNCC 4 cut(s) 66, 788, 869, 946
NmuCI GTSAC 3 cut(s) 470, 647, 683
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 1 cut(s) 248
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 5 cut(s) 16, 172, 379, 559, 812
PkrI GCNGC 3 cut(s) 568, 582, 935
PleI GAGTC 3 cut(s) 547, 614, 745
PpsI GAGTC 3 cut(s) 547, 614, 745
PshAI GACNNNNGTC 2 cut(s) 468, 645
PspN4I GGNNCC 4 cut(s) 66, 788, 869, 946
PspPI GGNCC 2 cut(s) 107, 483
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 3 cut(s) 6, 273, 944
RsaI GTAC 4 cut(s) 26, 66, 614, 983
RsaNI GTAC 4 cut(s) 25, 65, 613, 982
SaqAI TTAA 2 cut(s) 137, 422
SatI GCNGC 3 cut(s) 567, 581, 934
Sau3AI GATC 6 cut(s) 6, 273, 387, 435, 587, 944
Sau96I GGNCC 2 cut(s) 107, 483
SchI GAGTC 3 cut(s) 548, 614, 745
SduI GDGCHC 3 cut(s) 219, 844, 857
SfaNI GCATC 5 cut(s) 73, 187, 510, 755, 794
SinI GGWCC 1 cut(s) 483
SmlI CTYRAG 4 cut(s) 250, 536, 608, 816
SmoI CTYRAG 4 cut(s) 250, 536, 608, 816
Sse9I AATT 5 cut(s) 69, 260, 285, 657, 822
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 1 cut(s) 567
StuI AGGCCT 1 cut(s) 248
StyI CCWWGG 2 cut(s) 466, 713
TaaI ACNGT 2 cut(s) 335, 710
TaiI ACGT 2 cut(s) 987, 1017
TaqI TCGA 4 cut(s) 293, 586, 705, 810
TasI AATT 5 cut(s) 69, 260, 285, 657, 822
TatI WGTACW 1 cut(s) 612
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 5 cut(s) 16, 172, 379, 559, 812
Tru1I TTAA 2 cut(s) 137, 422
Tru9I TTAA 2 cut(s) 137, 422
TscAI CASTG 3 cut(s) 195, 421, 479
TseFI GTSAC 3 cut(s) 470, 647, 683
TseI GCWGC 2 cut(s) 580, 933
Tsp45I GTSAC 3 cut(s) 470, 647, 683
TspDTI ATGAA 7 cut(s) 51, 185, 296, 344, 539, 567, 572
TspRI CASTG 3 cut(s) 195, 421, 479
VpaK11BI GGWCC 1 cut(s) 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.