Rorug04G0346000

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000741
Physical Location & Seq
Forward (+)
51817008 .. 51822648
5641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0346000.1

Sequence Viewer

Length: 1542 bp
ATGCAGGTCAAAATCTTTGGTGTGCGAGGATGCTGTTTTGGCATTGCAAATATGATTCTTGTCAAGCGCATGAGGGAAACACTATACTCTTCACTTCTTGTTCAGGATATATTCTTTTTTGACACTGAAACAGTTGGTGATTTGACAAGTAGGCTTGGGGCAGATTGTCAGCAAGTGTCAAGAGTCATTGGAAATGATCTCAATTTCATATTGCGGAATGTTCTTCAGGGAGCAGGAGCAATGATCTACTTGTTAGTTTTATCACGTCCCCTTGGTTTATCTGTGTTGGTGATATGTTCTACTCTAGCAGCAATTATGCTGGTTTATGGCCGGTACCAGAAGAAGGCAGCAAAACTAACTCAGGAGTTTACTGCTTCTGCCAATGAGGTTGCACAAGAGGCATTCTCTTTGGTGAGAACTGTCCGTGTTTATGGAACTGAAAAACAAGAACTTGGAAGGTACAAACTGTGGTTAGATAAATTAGCTGATATAAGCATACGGCAAAGTGCTGCGTATGGTTTTTGGAACTTGAGCTTCAACGCTCTTTACCACTCAACCCAGGTCATTGCTGTGCTGCTTGGAGGGATGTCTATTCTATCTGGTCATATTACAGCAGAGAAACTGACGAAGTTTATATTGTATAGTGAGTGGTTAATTTATGCAACATGGTGGGTGGGTGACAATTTATCCAATCTGATGCAATCTGTTGGGGCAAGTGAAAAAGTCTTCCAATTAATGGATCTCATGCCCAGTGACCAATTTATATCAAAAGGATCAAAGTTGCAAAATCTGGTTGGAAATGTTGAGTTTGTAAACACTTCTTTCTATTATCCATCGAGGCCAACGGTCCCTGTACTGCAAAATATCAGCTTATCCGTGAAACCTAATGAAGTGGTTGCTATTGTTGGTCTTAGTGGTAGTGGAAAGAGCACACTTGTGAATCTTTTGCTCCGTCTCTATGAGCCAACAAATGGTCAGATTTTGATTGACGGCTGCCCTCTACAAGAGTTGGACGTCATGTGGTGGAGGGAAAGAATTGGATTTGTTGGACAGGAACCCAAACTGTTTCGCACAGACATTAGTTCAAACATTAGATATGGATGCACTAGAGATATCAGGCAGGAGGATGTTGAATGGGCTGCCAAGCAGGCATATGCACACGATTTCATTTTATCACTGCCCAAAGGTTATCAAACACTAGTTGATGATGATTTACTCAGTGGGGGGCAAAAGCAAAGAATTGCCATTGCGAGGGCCATTCTTAGGGACCCAACTATTTTGATCCTTGATGAAGCAACTAGTGCACTGGATGCAGAGAGTGAACACAATGTCAGGGGTGTTCTTCGTGCTGTCAGAAAAGACAAGACAACGAGGAGAAGTGTCATAATAATTGCTCACAGGCTTTCCACCATACAAGCTGCTGACAGGATAGTGGTCATGGATAATGGTAGAATTGTTGAGGCCGGCAGTCACAGTGAGCTGCTCGTTAAGGATGGCCTCTATGCACGACTAACTAGAAGACAAGCTGATGCAGTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

513

Amino Acids

57.25

Weight (kDa)

8.84

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 8 - 219 1e-32 ABC transporter transmembrane region
ABC_tran PF00005 286 - 434 2.3e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1017
Acc65I GGTACC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 333
AccB7I CCANNNNNTGG 2 cut(s) 736, 971
AciI CCGC 1 cut(s) 214
AclWI GGATC 3 cut(s) 747, 781, 1276
AcoI YGGCCR 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 1014
AfaI GTAC 3 cut(s) 335, 461, 855
AfiI CCNNNNNNNGG 5 cut(s) 343, 736, 971, 1251, 1263
AgsI TTSAA 3 cut(s) 538, 1086, 1133
AhlI ACTAGT 2 cut(s) 1198, 1298
AjiI CACGTC 1 cut(s) 266
AjnI CCWGG 1 cut(s) 558
AleI CACNNNNGTG 1 cut(s) 935
AluBI AGCT 6 cut(s) 485, 534, 870, 1418, 1480, 1526
AluI AGCT 6 cut(s) 485, 534, 870, 1418, 1480, 1526
Alw21I GWGCWC 2 cut(s) 932, 1306
Alw26I GTCTC 1 cut(s) 959
Alw44I GTGCAC 1 cut(s) 1302
AlwI GGATC 3 cut(s) 747, 781, 1276
AoxI GGCC 5 cut(s) 328, 839, 1254, 1461, 1495
ApaLI GTGCAC 1 cut(s) 1302
ApeKI GCWGC 8 cut(s) 308, 347, 509, 574, 993, 1139, 1418, 1480
AseI ATTAAT 1 cut(s) 734
Asp718I GGTACC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 847, 1254, 1267
AsuHPI GGTGA 4 cut(s) 149, 301, 424, 689
AvaII GGWCC 2 cut(s) 847, 1267
BaeGI GKGCMC 1 cut(s) 1306
BanI GGYRCC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 718, 1525
Bbv12I GWGCWC 2 cut(s) 932, 1306
BbvI GCAGC 8 cut(s) 320, 359, 496, 561, 980, 1126, 1405, 1467
BccI CCATC 2 cut(s) 841, 1487
BceAI ACGGC 2 cut(s) 515, 1006
BciT130I CCWGG 1 cut(s) 560
BcoDI GTCTC 1 cut(s) 959
BcuI ACTAGT 2 cut(s) 1198, 1298
BfaI CTAG 5 cut(s) 305, 1107, 1199, 1299, 1515
BglI GCCNNNNNGGC 1 cut(s) 1148
BisI GCNGC 8 cut(s) 309, 348, 510, 575, 994, 1140, 1419, 1481
BlsI GCNGC 8 cut(s) 310, 349, 511, 576, 995, 1141, 1420, 1482
Bme1390I CCNGG 1 cut(s) 560
Bme18I GGWCC 2 cut(s) 847, 1267
BmgBI CACGTC 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 847, 1254, 1267
BmiI GGNNCC 5 cut(s) 335, 849, 1056, 1268, 1269
BmrFI CCNGG 1 cut(s) 560
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 5 cut(s) 20, 687, 1091, 1300, 1519
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 718, 1525
BplI GAGNNNNNCTC 2 cut(s) 389, 421
BpuEI CTTGAG 1 cut(s) 550
BsaHI GRCGYC 1 cut(s) 1014
BsaJI CCNNGG 2 cut(s) 271, 558
Bsc4I CCNNNNNNNGG 5 cut(s) 343, 736, 971, 1251, 1263
Bse118I RCCGGY 2 cut(s) 330, 1463
Bse1I ACTGG 2 cut(s) 750, 1311
Bse3DI GCAATG 4 cut(s) 42, 246, 564, 1245
BseBI CCWGG 1 cut(s) 560
BseDI CCNNGG 2 cut(s) 271, 558
BseGI GGATG 6 cut(s) 35, 591, 1106, 1132, 1315, 1498
BseLI CCNNNNNNNGG 5 cut(s) 343, 736, 971, 1251, 1263
BseMI GCAATG 4 cut(s) 42, 246, 564, 1245
BseMII CTCAG 2 cut(s) 374, 1231
BseNI ACTGG 2 cut(s) 750, 1311
BseRI GAGGAG 1 cut(s) 1387
BseSI GKGCMC 1 cut(s) 1306
BseXI GCAGC 8 cut(s) 320, 359, 496, 561, 980, 1126, 1405, 1467
BshFI GGCC 5 cut(s) 330, 841, 1256, 1463, 1497
BshNI GGYRCC 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 932, 1306
BsiSI CCGG 2 cut(s) 331, 1464
BslFI GGGAC 3 cut(s) 252, 833, 1280
BslI CCNNNNNNNGG 5 cut(s) 343, 736, 971, 1251, 1263
BsmAI GTCTC 1 cut(s) 959
BsmBI CGTCTC 1 cut(s) 959
BsmFI GGGAC 3 cut(s) 252, 833, 1280
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 5 cut(s) 330, 841, 1256, 1463, 1497
Bsp1286I GDGCHC 2 cut(s) 932, 1306
Bsp143I GATC 5 cut(s) 196, 243, 739, 773, 1281
BspACI CCGC 1 cut(s) 214
BspANI GGCC 5 cut(s) 330, 841, 1256, 1463, 1497
BspCNI CTCAG 2 cut(s) 373, 1230
BspLI GGNNCC 5 cut(s) 335, 849, 1056, 1268, 1269
BspPI GGATC 3 cut(s) 747, 781, 1276
BspT107I GGYRCC 1 cut(s) 333
BsrDI GCAATG 4 cut(s) 42, 246, 564, 1245
BsrFI RCCGGY 2 cut(s) 330, 1463
BsrI ACTGG 2 cut(s) 750, 1311
BssAI RCCGGY 2 cut(s) 330, 1463
BssECI CCNNGG 2 cut(s) 271, 558
BssMI GATC 5 cut(s) 196, 243, 739, 773, 1281
BssNI GRCGYC 1 cut(s) 1014
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 560
Bst4CI ACNGT 6 cut(s) 133, 421, 468, 847, 1065, 1475
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 1014
BstAPI GCANNNNNTGC 2 cut(s) 1301, 1310
BstC8I GCNNGC 2 cut(s) 1149, 1465
BstDEI CTNAG 4 cut(s) 360, 911, 1217, 1262
BstF5I GGATG 6 cut(s) 35, 591, 1106, 1132, 1315, 1498
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 5 cut(s) 199, 246, 742, 776, 1284
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 5 cut(s) 196, 243, 739, 773, 1281
BstMWI GCNNNNNNNGC 5 cut(s) 39, 398, 1148, 1301, 1310
BstNI CCWGG 1 cut(s) 560
BstSCI CCNGG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 1306
BstV1I GCAGC 8 cut(s) 320, 359, 496, 561, 980, 1126, 1405, 1467
BstV2I GAAGAC 2 cut(s) 718, 1525
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BsuRI GGCC 5 cut(s) 330, 841, 1256, 1463, 1497
BtrI CACGTC 1 cut(s) 266
BtsCI GGATG 6 cut(s) 35, 591, 1106, 1132, 1315, 1498
BtsI GCAGTG 2 cut(s) 1175, 1539
BtsIMutI CAGTG 7 cut(s) 123, 757, 1175, 1225, 1304, 1480, 1539
Cac8I GCNNGC 2 cut(s) 1149, 1465
CfoI GCGC 1 cut(s) 69
Cfr10I RCCGGY 2 cut(s) 330, 1463
Cfr13I GGNCC 3 cut(s) 847, 1254, 1267
Csp6I GTAC 3 cut(s) 334, 460, 854
CviAII CATG 6 cut(s) 70, 666, 745, 1018, 1438, 1539
CviQI GTAC 3 cut(s) 334, 460, 854
DdeI CTNAG 4 cut(s) 360, 911, 1217, 1262
DpnI GATC 5 cut(s) 198, 245, 741, 775, 1283
DpnII GATC 5 cut(s) 196, 243, 739, 773, 1281
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 271
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 847, 1267
Eco57I CTGAAG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 1267
EcoRII CCWGG 1 cut(s) 558
EcoRV GATATC 1 cut(s) 1114
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 6 cut(s) 73, 669, 748, 1021, 1441, 1542
FaqI GGGAC 3 cut(s) 252, 833, 1280
FatI CATG 6 cut(s) 69, 665, 744, 1017, 1437, 1538
FauNDI CATATG 1 cut(s) 1153
Fnu4HI GCNGC 8 cut(s) 309, 348, 510, 575, 994, 1140, 1419, 1481
FokI GGATG 6 cut(s) 42, 598, 1113, 1139, 1322, 1505
Fsp4HI GCNGC 8 cut(s) 309, 348, 510, 575, 994, 1140, 1419, 1481
FspBI CTAG 5 cut(s) 305, 1107, 1199, 1299, 1515
GlaI GCGC 1 cut(s) 68
GluI GCNGC 8 cut(s) 309, 348, 510, 575, 994, 1140, 1419, 1481
HaeIII GGCC 5 cut(s) 330, 841, 1256, 1463, 1497
HapII CCGG 2 cut(s) 331, 1464
HhaI GCGC 1 cut(s) 69
Hin1I GRCGYC 1 cut(s) 1014
Hin1II CATG 6 cut(s) 73, 669, 748, 1021, 1441, 1542
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 3 cut(s) 55, 183, 940
HpaII CCGG 2 cut(s) 331, 1464
HphI GGTGA 4 cut(s) 149, 301, 424, 689
Hpy166II GTNNAC 4 cut(s) 369, 814, 1304, 1322
Hpy188I TCNGA 3 cut(s) 696, 978, 1355
Hpy188III TCNNGA 3 cut(s) 104, 180, 362
Hpy8I GTNNAC 4 cut(s) 369, 814, 1304, 1322
HpyAV CCTTC 2 cut(s) 337, 450
HpyCH4III ACNGT 6 cut(s) 133, 421, 468, 847, 1065, 1475
HpyCH4IV ACGT 2 cut(s) 265, 1014
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 398, 1148, 1301, 1310
HpyF3I CTNAG 4 cut(s) 360, 911, 1217, 1262
HpySE526I ACGT 2 cut(s) 265, 1014
Hsp92I GRCGYC 1 cut(s) 1014
Hsp92II CATG 6 cut(s) 73, 669, 748, 1021, 1441, 1542
HspAI GCGC 1 cut(s) 67
KflI GGGWCCC 1 cut(s) 1267
KpnI GGTACC 1 cut(s) 337
KroI GCCGGC 1 cut(s) 1463
KroNI GCCGGC 1 cut(s) 1465
Kzo9I GATC 5 cut(s) 196, 243, 739, 773, 1281
LmnI GCTCC 3 cut(s) 230, 236, 954
Lsp1109I GCAGC 8 cut(s) 320, 359, 496, 561, 980, 1126, 1405, 1467
LweI GCATC 5 cut(s) 20, 687, 1091, 1300, 1519
MaeI CTAG 5 cut(s) 305, 1107, 1199, 1299, 1515
MaeII ACGT 2 cut(s) 265, 1014
MaeIII GTNAC 3 cut(s) 677, 752, 1469
MalI GATC 5 cut(s) 198, 245, 741, 775, 1283
MboI GATC 5 cut(s) 196, 243, 739, 773, 1281
MboII GAAGA 6 cut(s) 81, 215, 352, 718, 1334, 1530
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 2 cut(s) 932, 1306
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 3 cut(s) 775, 990, 1027
MroNI GCCGGC 1 cut(s) 1463
MseI TTAA 3 cut(s) 653, 734, 1488
MslI CAYNNNNRTG 2 cut(s) 569, 935
MspI CCGG 2 cut(s) 331, 1464
MspR9I CCNGG 1 cut(s) 560
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 560
MwoI GCNNNNNNNGC 5 cut(s) 39, 398, 1148, 1301, 1310
NaeI GCCGGC 1 cut(s) 1465
NdeI CATATG 1 cut(s) 1153
NdeII GATC 5 cut(s) 196, 243, 739, 773, 1281
NgoMIV GCCGGC 1 cut(s) 1463
NlaIII CATG 6 cut(s) 73, 669, 748, 1021, 1441, 1542
NlaIV GGNNCC 5 cut(s) 335, 849, 1056, 1268, 1269
NmuCI GTSAC 3 cut(s) 677, 752, 1469
OliI CACNNNNGTG 1 cut(s) 935
PctI GAATGC 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 1465
PfeI GAWTC 2 cut(s) 55, 940
PflMI CCANNNNNTGG 2 cut(s) 736, 971
PkrI GCNGC 8 cut(s) 310, 349, 511, 576, 995, 1141, 1420, 1482
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 1267
PshBI ATTAAT 1 cut(s) 734
Psp5II RGGWCCY 1 cut(s) 1267
Psp6I CCWGG 1 cut(s) 558
PspGI CCWGG 1 cut(s) 558
PspN4I GGNNCC 5 cut(s) 335, 849, 1056, 1268, 1269
PspPI GGNCC 3 cut(s) 847, 1254, 1267
PspPPI RGGWCCY 1 cut(s) 1267
PsuI RGATCY 1 cut(s) 739
RsaI GTAC 3 cut(s) 335, 461, 855
RsaNI GTAC 3 cut(s) 334, 460, 854
RseI CAYNNNNRTG 2 cut(s) 569, 935
SaqAI TTAA 3 cut(s) 653, 734, 1488
SatI GCNGC 8 cut(s) 309, 348, 510, 575, 994, 1140, 1419, 1481
Sau3AI GATC 5 cut(s) 196, 243, 739, 773, 1281
Sau96I GGNCC 3 cut(s) 847, 1254, 1267
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 1 cut(s) 560
SduI GDGCHC 2 cut(s) 932, 1306
SfaNI GCATC 5 cut(s) 20, 687, 1091, 1300, 1519
SinI GGWCC 2 cut(s) 847, 1267
SmiMI CAYNNNNRTG 2 cut(s) 569, 935
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SpeI ACTAGT 2 cut(s) 1198, 1298
SsiI CCGC 1 cut(s) 214
SspMI CTAG 5 cut(s) 305, 1107, 1199, 1299, 1515
StyD4I CCNGG 1 cut(s) 558
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 6 cut(s) 133, 421, 468, 847, 1065, 1475
TaiI ACGT 2 cut(s) 268, 1017
TaqI TCGA 1 cut(s) 836
TatI WGTACW 1 cut(s) 853
TfiI GAWTC 2 cut(s) 55, 940
Tru1I TTAA 3 cut(s) 653, 734, 1488
Tru9I TTAA 3 cut(s) 653, 734, 1488
TscAI CASTG 7 cut(s) 130, 757, 1182, 1225, 1311, 1480, 1539
TseFI GTSAC 3 cut(s) 677, 752, 1469
TseI GCWGC 8 cut(s) 308, 347, 509, 574, 993, 1139, 1418, 1480
Tsp45I GTSAC 3 cut(s) 677, 752, 1469
TspDTI ATGAA 4 cut(s) 196, 903, 1156, 1305
TspGWI ACGGA 3 cut(s) 413, 865, 941
TspRI CASTG 7 cut(s) 130, 757, 1182, 1225, 1311, 1480, 1539
Van91I CCANNNNNTGG 2 cut(s) 736, 971
VneI GTGCAC 1 cut(s) 1302
VpaK11BI GGWCC 2 cut(s) 847, 1267
VspI ATTAAT 1 cut(s) 734
XspI CTAG 5 cut(s) 305, 1107, 1199, 1299, 1515
ZraI GACGTC 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.