Rroxscaffold_5G00383970

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000741
Physical Location & Seq
Reverse (-)
63518813 .. 63520262
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383970.1

Sequence Viewer

Length: 615 bp
ATGTACTCGTCAAAGCCGAAATCGTACGAGAAGTTCTCAGAGTCCAACACCGATGCTGCTTCTCCGCCGACGGGTATTCCGGTCAGCTCCAGCAACCCTTACTTCAGTCATGATGATCATGATCATGATCATGAAAACAGCTCCCAAATGCCGGCTGCTCCGCCATACCACCTCCGCCCTAGAGCCCCCGAGCCGTGGAAGACCGGCCTCTGCGACTGCCTCTCGGATCCCAAAAACTGCTGCACAACATTTTGGTGCCCTTGTATCACTTTCGGCCAGATTGCAGAGATTGTGGATAAGGGCTCTACTTCTTGTGGTGCAAGTGGAGCACTATACACTCTGATATTGTGTGTGACTGGTTGTCCTTGCTTCTATTCATGCTTCTACCGTTCCAAAATGAGGCAGCAATATACTCTTGATGAGAGCCCTTGTGGTGATTGCTTGGTTCATTGCTTCTGCGAGTACTGCGCCCTGTGCCAGGAGTACCGTGAGCTTCAAAATCGCGGTTTCGACATGGTTATCGGATGGCATGGAAATATCGAGGAAAGGAACCGTGAAGTGGCAATGAATCCAGTGCCCCCGGTCATGGAGGAAGGCATGAGTCGAGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

204

Amino Acids

22.93

Weight (kDa)

5.07

Isoelectric Point (pI)

62.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 66 - 164 3.4e-35 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 255
AccII CGCG 1 cut(s) 504
AciI CCGC 4 cut(s) 65, 161, 175, 504
AclWI GGATC 2 cut(s) 221, 234
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 88
AfaI GTAC 4 cut(s) 5, 26, 464, 485
AfiI CCNNNNNNNGG 7 cut(s) 71, 151, 195, 399, 478, 559, 586
AgsI TTSAA 1 cut(s) 497
AhdI GACNNNNNGTC 1 cut(s) 360
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 3 cut(s) 87, 141, 493
AluI AGCT 3 cut(s) 87, 141, 493
Alw21I GWGCWC 1 cut(s) 331
AlwI GGATC 2 cut(s) 221, 234
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 205, 274
ApeKI GCWGC 4 cut(s) 56, 155, 240, 403
AspLEI GCGC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 188
BaeGI GKGCMC 2 cut(s) 260, 579
BamHI GGATCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 255
BanII GRGCYC 3 cut(s) 187, 305, 428
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 43, 142, 227, 415
BccI CCATC 1 cut(s) 519
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 1 cut(s) 479
BclI TGATCA 3 cut(s) 115, 121, 127
BcnI CCSGG 1 cut(s) 581
BfaI CTAG 1 cut(s) 180
BisI GCNGC 4 cut(s) 57, 156, 241, 404
BlsI GCNGC 4 cut(s) 58, 157, 242, 405
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 2 cut(s) 479, 581
BmeRI GACNNNNNGTC 1 cut(s) 360
BmeT110I CYCGRG 1 cut(s) 188
BmiI GGNNCC 3 cut(s) 228, 257, 551
BmrFI CCNGG 2 cut(s) 479, 581
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 581
BsaBI GATNNNNATC 2 cut(s) 120, 126
BsaJI CCNNGG 2 cut(s) 194, 579
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 151, 195, 399, 478, 559, 586
Bse118I RCCGGY 2 cut(s) 151, 203
Bse1I ACTGG 2 cut(s) 361, 572
Bse3DI GCAATG 2 cut(s) 448, 570
Bse8I GATNNNNATC 2 cut(s) 120, 126
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 194, 579
BseGI GGATG 1 cut(s) 530
BseJI GATNNNNATC 2 cut(s) 120, 126
BseLI CCNNNNNNNGG 7 cut(s) 71, 151, 195, 399, 478, 559, 586
BseMI GCAATG 2 cut(s) 448, 570
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 2 cut(s) 361, 572
BseSI GKGCMC 2 cut(s) 260, 579
BseXI GCAGC 4 cut(s) 43, 142, 227, 415
BsgI GTGCAG 1 cut(s) 226
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 207, 276
BshNI GGYRCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 80, 152, 204, 581
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 7 cut(s) 71, 151, 195, 399, 478, 559, 586
BsnI GGCC 2 cut(s) 207, 276
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 6 cut(s) 187, 260, 305, 331, 428, 579
Bsp143I GATC 5 cut(s) 115, 121, 127, 226, 607
BspACI CCGC 4 cut(s) 65, 161, 175, 504
BspANI GGCC 2 cut(s) 207, 276
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 1 cut(s) 504
BspHI TCATGA 4 cut(s) 109, 118, 124, 130
BspLI GGNNCC 3 cut(s) 228, 257, 551
BspPI GGATC 2 cut(s) 221, 234
BspT107I GGYRCC 1 cut(s) 255
BsrDI GCAATG 2 cut(s) 448, 570
BsrFI RCCGGY 2 cut(s) 151, 203
BsrI ACTGG 2 cut(s) 361, 572
BssAI RCCGGY 2 cut(s) 151, 203
BssECI CCNNGG 2 cut(s) 194, 579
BssMI GATC 5 cut(s) 115, 121, 127, 226, 607
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 389, 488, 554
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 194
BstENI CCTNNNNNAGG 1 cut(s) 476
BstF5I GGATG 1 cut(s) 530
BstFNI CGCG 1 cut(s) 504
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 5 cut(s) 118, 124, 130, 229, 610
BstMBI GATC 5 cut(s) 115, 121, 127, 226, 607
BstMWI GCNNNNNNNGC 3 cut(s) 326, 465, 474
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 477, 579
BstSLI GKGCMC 2 cut(s) 260, 579
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 4 cut(s) 43, 142, 227, 415
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 2 cut(s) 207, 276
BtgI CCRYGG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 530
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 1 cut(s) 153
CciI TCATGA 4 cut(s) 109, 118, 124, 130
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 2 cut(s) 151, 203
Csp6I GTAC 4 cut(s) 4, 25, 463, 484
CviAII CATG 9 cut(s) 110, 119, 125, 131, 378, 514, 530, 586, 598
CviQI GTAC 4 cut(s) 4, 25, 463, 484
DdeI CTNAG 1 cut(s) 37
DpnI GATC 5 cut(s) 117, 123, 129, 228, 609
DpnII GATC 5 cut(s) 115, 121, 127, 226, 607
DriI GACNNNNNGTC 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 274
Eam1105I GACNNNNNGTC 1 cut(s) 360
EciI GGCGGA 3 cut(s) 54, 150, 164
Eco24I GRGCYC 3 cut(s) 187, 305, 428
Eco57I CTGAAG 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 477
EcoT38I GRGCYC 3 cut(s) 187, 305, 428
FaeI CATG 9 cut(s) 113, 122, 128, 134, 381, 517, 533, 589, 601
FatI CATG 9 cut(s) 109, 118, 124, 130, 377, 513, 529, 585, 597
FbaI TGATCA 3 cut(s) 115, 121, 127
Fnu4HI GCNGC 4 cut(s) 57, 156, 241, 404
FokI GGATG 1 cut(s) 537
FriOI GRGCYC 3 cut(s) 187, 305, 428
Fsp4HI GCNGC 4 cut(s) 57, 156, 241, 404
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 469
GluI GCNGC 4 cut(s) 57, 156, 241, 404
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 2 cut(s) 207, 276
HapII CCGG 4 cut(s) 80, 152, 204, 581
HhaI GCGC 1 cut(s) 470
Hin1II CATG 9 cut(s) 113, 122, 128, 134, 381, 517, 533, 589, 601
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HinfI GANTC 3 cut(s) 41, 568, 601
HpaII CCGG 4 cut(s) 80, 152, 204, 581
HphI GGTGA 1 cut(s) 446
Hpy188I TCNGA 4 cut(s) 40, 226, 342, 524
Hpy188III TCNNGA 6 cut(s) 110, 119, 125, 131, 416, 605
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 587
HpyCH4III ACNGT 3 cut(s) 389, 488, 554
HpyCH4V TGCA 3 cut(s) 243, 284, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 465, 474
HpyF3I CTNAG 1 cut(s) 37
Hsp92II CATG 9 cut(s) 113, 122, 128, 134, 381, 517, 533, 589, 601
HspAI GCGC 1 cut(s) 468
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Ksp22I TGATCA 3 cut(s) 115, 121, 127
Kzo9I GATC 5 cut(s) 115, 121, 127, 226, 607
LmnI GCTCC 4 cut(s) 92, 146, 163, 326
Lsp1109I GCAGC 4 cut(s) 43, 142, 227, 415
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 352
MalI GATC 5 cut(s) 117, 123, 129, 228, 609
MboI GATC 5 cut(s) 115, 121, 127, 226, 607
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 6 cut(s) 187, 260, 305, 331, 428, 579
MlyI GAGTC 2 cut(s) 50, 610
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 6 cut(s) 182, 218, 230, 393, 535, 583
MroNI GCCGGC 1 cut(s) 151
MslI CAYNNNNRTG 3 cut(s) 123, 129, 253
MspI CCGG 4 cut(s) 80, 152, 204, 581
MspR9I CCNGG 2 cut(s) 479, 581
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 326, 465, 474
NaeI GCCGGC 1 cut(s) 153
NciI CCSGG 1 cut(s) 581
NdeII GATC 5 cut(s) 115, 121, 127, 226, 607
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 9 cut(s) 113, 122, 128, 134, 381, 517, 533, 589, 601
NlaIV GGNNCC 3 cut(s) 228, 257, 551
NmuCI GTSAC 1 cut(s) 352
PagI TCATGA 4 cut(s) 109, 118, 124, 130
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 568
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 4 cut(s) 58, 157, 242, 405
PleI GAGTC 2 cut(s) 49, 609
PpsI GAGTC 2 cut(s) 49, 609
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 228, 257, 551
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 4 cut(s) 5, 26, 464, 485
RsaNI GTAC 4 cut(s) 4, 25, 463, 484
RseI CAYNNNNRTG 3 cut(s) 123, 129, 253
SatI GCNGC 4 cut(s) 57, 156, 241, 404
Sau3AI GATC 5 cut(s) 115, 121, 127, 226, 607
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 2 cut(s) 50, 610
ScrFI CCNGG 2 cut(s) 479, 581
SduI GDGCHC 6 cut(s) 187, 260, 305, 331, 428, 579
SetI ASST 4 cut(s) 89, 143, 174, 495
SfaNI GCATC 1 cut(s) 43
SmiMI CAYNNNNRTG 3 cut(s) 123, 129, 253
SsiI CCGC 4 cut(s) 65, 161, 175, 504
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 477, 579
TaaI ACNGT 3 cut(s) 389, 488, 554
TaqI TCGA 3 cut(s) 510, 540, 604
TatI WGTACW 2 cut(s) 3, 462
TfiI GAWTC 1 cut(s) 568
TscAI CASTG 1 cut(s) 579
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 4 cut(s) 56, 155, 240, 403
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 147, 366, 437, 581
TspRI CASTG 1 cut(s) 579
XagI CCTNNNNNAGG 1 cut(s) 476
XspI CTAG 1 cut(s) 180
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.