Rh4AG401800

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000741
Physical Location & Seq
Reverse (-)
70450827 .. 70452287
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG401800.1

Sequence Viewer

Length: 600 bp
ATGTCCTCATCAAATCCAAATTCTTACGTAAAGTTCTCCGAGACCAGTGCACTACCGGCGGTAGGTGTTCCGGTGAGCTCCAGCAACCCGTATTTTAGTCAGCCTGCTCATCATCATGACAACTTTAACTCCCACATGCCCGCTGCTCCCCCATATCATCTCCATCCTACTGCCCCCGAGCCTTGGAAATCTGGAATATTTGACTGCTTCTCCGATCCTCAAAGCTGCTGCATAACACTGTGCTGTCCATGCATCACTTTTGGCCAGATTGCCGAGATCGTAGATAAAGGCTCTACATCTTGCGGGGTAAGTGGAGCAATTTACGCCGTGATTGCTTATATGACTGGTTGTGCCTGCATCTATTCATGCTTCTACCGCTCCAAACTGAGGCACCAGTATTCTCTGGACGAGAGTCCTTGTGGTGATTGTTTGGTTCACTGCTTCTGCGAGCCCTGCGCCTTGTGCCAGGAGTACCGTGAACTCCAAAGTCGCGGTTTCAACATGGCTATCGGATGGCATGGAAATATGGATGGGAGGAACCGTGAAATGGCAATGGCTTCAATCCCCCCAATGATGGAGGGAGGCATGAGTCGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

199

Amino Acids

21.81

Weight (kDa)

5.94

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 62 - 160 1.8e-36 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 390
AccBSI CCGCTC 1 cut(s) 378
AccII CGCG 1 cut(s) 492
AciI CCGC 5 cut(s) 59, 141, 303, 376, 492
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 19
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 5 cut(s) 62, 183, 387, 547, 574
AgsI TTSAA 2 cut(s) 499, 561
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 2 cut(s) 78, 225
AluI AGCT 2 cut(s) 78, 225
Alw21I GWGCWC 2 cut(s) 52, 80
Alw26I GTCTC 1 cut(s) 35
Alw44I GTGCAC 1 cut(s) 48
AlwI GGATC 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 262
ApaLI GTGCAC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 143, 225, 228
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 85, 434
AvaI CYCGRG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 52
BalI TGGCCA 1 cut(s) 264
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 2 cut(s) 80, 453
Bbv12I GWGCWC 2 cut(s) 52, 80
BbvI GCAGC 3 cut(s) 130, 212, 215
BccI CCATC 4 cut(s) 171, 507, 524, 568
BceAI ACGGC 1 cut(s) 311
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 1 cut(s) 467
BcoDI GTCTC 1 cut(s) 35
BisI GCNGC 3 cut(s) 144, 226, 229
BlsI GCNGC 3 cut(s) 145, 227, 230
Bme1390I CCNGG 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 392, 539
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 2 cut(s) 261, 366
BoxI GACNNNNGTC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 28
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 194, 224, 526, 556
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 183, 387, 547, 574
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 45, 349, 394
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 3 cut(s) 163, 518, 535
BseLI CCNNNNNNNGG 5 cut(s) 62, 183, 387, 547, 574
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 3 cut(s) 45, 349, 394
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 3 cut(s) 130, 212, 215
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 390
BsiHKAI GWGCWC 2 cut(s) 52, 80
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 56, 71
BslI CCNNNNNNNGG 5 cut(s) 62, 183, 387, 547, 574
BsmAI GTCTC 1 cut(s) 35
BsnI GGCC 1 cut(s) 264
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 52, 80, 453
Bsp143I GATC 2 cut(s) 214, 276
BspACI CCGC 5 cut(s) 59, 141, 303, 376, 492
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 378
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 392, 539
BspPI GGATC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 45, 349, 394
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 2 cut(s) 214, 276
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 3 cut(s) 240, 476, 542
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 4 cut(s) 105, 141, 355, 449
BstDEI CTNAG 1 cut(s) 386
BstF5I GGATG 3 cut(s) 163, 518, 535
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 2 cut(s) 217, 279
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 2 cut(s) 214, 276
BstMWI GCNNNNNNNGC 7 cut(s) 56, 249, 323, 332, 375, 453, 462
BstNI CCWGG 1 cut(s) 467
BstNSI RCATGY 1 cut(s) 139
BstPAI GACNNNNGTC 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 52
BstSNI TACGTA 1 cut(s) 28
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 130, 212, 215
BsuRI GGCC 1 cut(s) 264
BtsCI GGATG 3 cut(s) 163, 518, 535
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 52, 236, 436
Cac8I GCNNGC 4 cut(s) 105, 141, 355, 449
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 55
Csp6I GTAC 1 cut(s) 472
CviAII CATG 7 cut(s) 116, 136, 249, 366, 502, 518, 586
CviJI RGCY 9 cut(s) 78, 103, 181, 225, 264, 291, 451, 506, 557
CviKI_1 RGCY 9 cut(s) 78, 103, 181, 225, 264, 291, 451, 506, 557
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 1 cut(s) 386
DpnI GATC 2 cut(s) 216, 278
DpnII GATC 2 cut(s) 214, 276
EaeI YGGCCR 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 78
Eco105I TACGTA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 2 cut(s) 80, 453
Eco31I GGTCTC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 80, 453
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 7 cut(s) 119, 139, 252, 369, 505, 521, 589
FatI CATG 7 cut(s) 115, 135, 248, 365, 501, 517, 585
FauI CCCGC 2 cut(s) 148, 296
Fnu4HI GCNGC 3 cut(s) 144, 226, 229
FokI GGATG 3 cut(s) 150, 525, 542
FriOI GRGCYC 2 cut(s) 80, 453
Fsp4HI GCNGC 3 cut(s) 144, 226, 229
GlaI GCGC 1 cut(s) 457
GluI GCNGC 3 cut(s) 144, 226, 229
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 1 cut(s) 264
HapII CCGG 2 cut(s) 56, 71
HhaI GCGC 1 cut(s) 458
Hin1II CATG 7 cut(s) 119, 139, 252, 369, 505, 521, 589
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 2 cut(s) 412, 589
HpaII CCGG 2 cut(s) 56, 71
HphI GGTGA 2 cut(s) 85, 434
Hpy166II GTNNAC 3 cut(s) 50, 436, 479
Hpy188I TCNGA 3 cut(s) 40, 214, 512
Hpy188III TCNNGA 3 cut(s) 116, 192, 404
Hpy8I GTNNAC 3 cut(s) 50, 436, 479
HpyCH4III ACNGT 3 cut(s) 240, 476, 542
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 4 cut(s) 50, 231, 252, 357
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 249, 323, 332, 375, 453, 462
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 7 cut(s) 119, 139, 252, 369, 505, 521, 589
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 2 cut(s) 214, 276
LmnI GCTCC 4 cut(s) 83, 151, 314, 383
Lsp1109I GCAGC 3 cut(s) 130, 212, 215
LweI GCATC 2 cut(s) 261, 366
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 216, 278
MbiI CCGCTC 1 cut(s) 378
MboI GATC 2 cut(s) 214, 276
MhlI GDGCHC 3 cut(s) 52, 80, 453
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 2 cut(s) 19, 318
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 421, 598
MnlI CCTC 6 cut(s) 16, 228, 381, 528, 571, 575
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 1 cut(s) 254
MscI TGGCCA 1 cut(s) 264
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 56, 71
MspR9I CCNGG 1 cut(s) 467
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 7 cut(s) 56, 249, 323, 332, 375, 453, 462
NdeII GATC 2 cut(s) 214, 276
NlaIII CATG 7 cut(s) 119, 139, 252, 369, 505, 521, 589
NlaIV GGNNCC 2 cut(s) 392, 539
NmeAIII GCCGAG 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 139
PagI TCATGA 1 cut(s) 115
PkrI GCNGC 3 cut(s) 145, 227, 230
PleI GAGTC 2 cut(s) 420, 597
PpsI GAGTC 2 cut(s) 420, 597
Ppu21I YACGTR 1 cut(s) 28
PshAI GACNNNNGTC 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 2 cut(s) 392, 539
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 114
SacI GAGCTC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 3 cut(s) 144, 226, 229
Sau3AI GATC 2 cut(s) 214, 276
SchI GAGTC 2 cut(s) 421, 598
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 3 cut(s) 52, 80, 453
SetI ASST 4 cut(s) 30, 67, 80, 227
SfaNI GCATC 2 cut(s) 261, 366
SmiMI CAYNNNNRTG 1 cut(s) 114
SnaBI TACGTA 1 cut(s) 28
Sse9I AATT 2 cut(s) 19, 318
SsiI CCGC 5 cut(s) 59, 141, 303, 376, 492
SspI AATATT 1 cut(s) 198
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 3 cut(s) 240, 476, 542
TaiI ACGT 1 cut(s) 30
TasI AATT 2 cut(s) 19, 318
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 3 cut(s) 52, 243, 443
TseI GCWGC 3 cut(s) 143, 225, 228
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 3 cut(s) 52, 243, 443
VneI GTGCAC 1 cut(s) 48
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.