RchiOBHm_Chr4g0443421

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000741
Physical Location & Seq
Reverse (-)
64548687 .. 64549885
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41112

Sequence Viewer

Length: 366 bp
ATGTTGTCCGGCGTGTGGTGGTGGGCTGGAACAATAATGATGGAGCTGCGAGGTTGGGCCCGCTTTTGGGGAAGGATTTTAGCAGAGGTGGTCACCAAAATTAACCTTGCAAGCTTCGGACCTTTTGTGGCGGTGCTAGGAAAGCCGGAGTCGACTCTGACTGATATGAAAACGAAGCAGCGGTGCATCCCAGAGTCTGTGGTTTCCGCGTTATTAGAAAAGAAAGAAAACGGATTACGACACGCGGAGACTTGTAGTCAAGTCAGGCCATGCAGAGGCCCTACTTCAATATCAAATACTTTGAATTTTCCAAGTCCTCTCTACCTTATGGTCCTCATCTGTTTACCTCGATCGAGGCTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.56

Weight (kDa)

9.76

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 152
AccII CGCG 2 cut(s) 209, 245
AciI CCGC 5 cut(s) 61, 131, 181, 207, 245
AcsI RAATTY 1 cut(s) 304
AfiI CCNNNNNNNGG 4 cut(s) 15, 66, 67, 275
AgsI TTSAA 2 cut(s) 288, 304
AhdI GACNNNNNGTC 1 cut(s) 255
AluBI AGCT 3 cut(s) 46, 114, 362
AluI AGCT 3 cut(s) 46, 114, 362
Alw26I GTCTC 1 cut(s) 242
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 3 cut(s) 57, 266, 277
ApaI GGGCCC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 46, 178
ApoI RAATTY 1 cut(s) 304
AspS9I GGNCC 5 cut(s) 57, 58, 119, 278, 331
AsuHPI GGTGA 1 cut(s) 85
AsuNHI GCTAGC 1 cut(s) 358
AvaII GGWCC 2 cut(s) 119, 331
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 33, 190
BccI CCATC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 137, 359
BisI GCNGC 2 cut(s) 47, 179
BlsI GCNGC 2 cut(s) 48, 180
Bme18I GGWCC 2 cut(s) 119, 331
BmeRI GACNNNNNGTC 1 cut(s) 255
BmgT120I GGNCC 5 cut(s) 57, 58, 119, 278, 331
BmiI GGNNCC 1 cut(s) 59
BmsI GCATC 1 cut(s) 195
BmtI GCTAGC 1 cut(s) 362
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 66, 67, 275
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 4 cut(s) 15, 66, 67, 275
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 2 cut(s) 33, 190
Bsh1236I CGCG 2 cut(s) 209, 245
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 3 cut(s) 59, 268, 279
BsiEI CGRYCG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 9, 146
BslI CCNNNNNNNGG 4 cut(s) 15, 66, 67, 275
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 3 cut(s) 59, 268, 279
Bsp120I GGGCCC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 350
BspACI CCGC 5 cut(s) 61, 131, 181, 207, 245
BspANI GGCC 3 cut(s) 59, 268, 279
BspFNI CGCG 2 cut(s) 209, 245
BspLI GGNNCC 1 cut(s) 59
BspOI GCTAGC 1 cut(s) 362
BssMI GATC 1 cut(s) 350
BstC8I GCNNGC 3 cut(s) 61, 112, 360
BstEII GGTNACC 1 cut(s) 91
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 2 cut(s) 209, 245
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 1 cut(s) 350
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstPI GGTNACC 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 2 cut(s) 209, 245
BstV1I GCAGC 2 cut(s) 33, 190
BsuRI GGCC 3 cut(s) 59, 268, 279
BtsCI GGATG 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 61, 112, 360
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 5 cut(s) 57, 58, 119, 278, 331
CviAII CATG 1 cut(s) 270
CviJI RGCY 9 cut(s) 26, 46, 59, 114, 145, 268, 279, 358, 362
CviKI_1 RGCY 9 cut(s) 26, 46, 59, 114, 145, 268, 279, 358, 362
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
DriI GACNNNNNGTC 1 cut(s) 255
Eam1105I GACNNNNNGTC 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 119, 331
Eco91I GGTNACC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 278
EcoO65I GGTNACC 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 1 cut(s) 273
FaiI YATR 3 cut(s) 167, 271, 329
FatI CATG 1 cut(s) 269
FauI CCCGC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 2 cut(s) 47, 179
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 47, 179
FspBI CTAG 2 cut(s) 137, 359
GluI GCNGC 2 cut(s) 47, 179
HaeIII GGCC 3 cut(s) 59, 268, 279
HapII CCGG 2 cut(s) 9, 146
Hin1II CATG 1 cut(s) 273
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 3 cut(s) 149, 154, 194
HpaII CCGG 2 cut(s) 9, 146
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 153, 344
Hpy188I TCNGA 2 cut(s) 119, 159
Hpy8I GTNNAC 2 cut(s) 153, 344
HpyAV CCTTC 1 cut(s) 66
HpyCH4V TGCA 3 cut(s) 110, 186, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
Hsp92II CATG 1 cut(s) 273
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 5 cut(s) 12, 22, 159, 204, 250
Lsp1109I GCAGC 2 cut(s) 33, 190
LweI GCATC 1 cut(s) 195
MaeI CTAG 2 cut(s) 137, 359
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 2 cut(s) 99, 304
MlyI GAGTC 3 cut(s) 148, 158, 203
MnlI CCTC 7 cut(s) 44, 79, 269, 327, 344, 348, 357
MseI TTAA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 2 cut(s) 9, 146
MvnI CGCG 2 cut(s) 209, 245
MwoI GCNNNNNNNGC 1 cut(s) 142
NdeII GATC 1 cut(s) 350
NheI GCTAGC 1 cut(s) 358
NlaIII CATG 1 cut(s) 273
NlaIV GGNNCC 1 cut(s) 59
NmuCI GTSAC 1 cut(s) 91
PkrI GCNGC 2 cut(s) 48, 180
Ple19I CGATCG 1 cut(s) 353
PleI GAGTC 3 cut(s) 148, 157, 202
PpsI GAGTC 3 cut(s) 148, 157, 202
PspEI GGTNACC 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 59
PspOMI GGGCCC 1 cut(s) 57
PspPI GGNCC 5 cut(s) 57, 58, 119, 278, 331
PstNI CAGNNNCTG 1 cut(s) 197
PvuI CGATCG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 47, 179
Sau3AI GATC 1 cut(s) 350
Sau96I GGNCC 5 cut(s) 57, 58, 119, 278, 331
SchI GAGTC 3 cut(s) 148, 158, 203
SduI GDGCHC 1 cut(s) 61
SetI ASST 9 cut(s) 48, 55, 90, 108, 116, 124, 327, 349, 364
SfaNI GCATC 1 cut(s) 195
SinI GGWCC 2 cut(s) 119, 331
Sse9I AATT 2 cut(s) 99, 304
SsiI CCGC 5 cut(s) 61, 131, 181, 207, 245
SspMI CTAG 2 cut(s) 137, 359
TaqI TCGA 3 cut(s) 152, 349, 353
TasI AATT 2 cut(s) 99, 304
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 2 cut(s) 46, 178
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 182
TspGWI ACGGA 1 cut(s) 246
VpaK11BI GGWCC 2 cut(s) 119, 331
XapI RAATTY 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 2 cut(s) 137, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.