Rh4AG154100

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000741
Physical Location & Seq
Reverse (-)
38924370 .. 38925807
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG154100.1

Sequence Viewer

Length: 408 bp
ATGTCCTCATCAAACTTCCACAAGCAGGATGCTCCTAGAACCCCCGAGCCTTGGAAATCTGGCCTCCTTGACTGCTTCTCCGATCCCAAAAGCTGTTGCTTAACATATTGGTGTCCATGCATCACTTTTGGCCGGATTGCTGAGATTGTGGATAAAGGCTCTCCATCTTGTGCTGCAAGTGGAGCAATGTACACGTTCATAGCTATCGTGATTGGTATTCCTTGCTGGTATTCATGCTCCTACCGCATGAAGATGAGGGAGCAGTACGCTTTGGACGGGAACCGTTGTAGTGATTTTTTGATTCACTGCTTCTGTGAGAACTGCGCCTTGTGCCAGGAGTATCGTGAGCTCCAAAGTCGCGGTTTTAACATGGCCATCGGTATCTCATTTTGTCAAATCTCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

135

Amino Acids

15.27

Weight (kDa)

6.67

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 18 - 116 9.4e-34 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 360
AciI CCGC 2 cut(s) 244, 360
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 2 cut(s) 130, 372
AfaI GTAC 2 cut(s) 191, 266
AfiI CCNNNNNNNGG 2 cut(s) 25, 51
AflIII ACRYGT 1 cut(s) 192
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 3 cut(s) 93, 203, 349
AluI AGCT 3 cut(s) 93, 203, 349
Alw21I GWGCWC 1 cut(s) 351
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 3 cut(s) 61, 130, 372
ApeKI GCWGC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 326
AvaI CYCGRG 1 cut(s) 44
BalI TGGCCA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 160
BccI CCATC 2 cut(s) 172, 383
BciT130I CCWGG 1 cut(s) 335
BfaI CTAG 1 cut(s) 36
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 19, 129
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 51
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 25, 51
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 63, 132, 374
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 25, 51
BsnI GGCC 3 cut(s) 63, 132, 374
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 351
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 244, 360
BspANI GGCC 3 cut(s) 63, 132, 374
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 281
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 192
BsrGI TGTACA 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 182, 243, 330
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 333
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 160
BsuRI GGCC 3 cut(s) 63, 132, 374
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
CfoI GCGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 190, 265
CviAII CATG 4 cut(s) 117, 234, 247, 370
CviJI RGCY 8 cut(s) 49, 63, 93, 132, 159, 203, 349, 374
CviKI_1 RGCY 8 cut(s) 49, 63, 93, 132, 159, 203, 349, 374
CviQI GTAC 2 cut(s) 190, 265
DdeI CTNAG 1 cut(s) 141
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
EaeI YGGCCR 2 cut(s) 130, 372
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 120, 237, 250, 373
FaiI YATR 6 cut(s) 106, 118, 200, 235, 248, 371
FatI CATG 4 cut(s) 116, 233, 246, 369
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 41
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 3 cut(s) 63, 132, 374
HapII CCGG 1 cut(s) 133
HhaI GCGC 1 cut(s) 326
Hin1II CATG 4 cut(s) 120, 237, 250, 373
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 1 cut(s) 301
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 2 cut(s) 208, 344
Hpy8I GTNNAC 1 cut(s) 192
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 2 cut(s) 120, 176
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 243, 330
HpyF3I CTNAG 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 194
Hsp92II CATG 4 cut(s) 120, 237, 250, 373
HspAI GCGC 1 cut(s) 324
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 5 cut(s) 37, 182, 242, 259, 354
LpnPI CCDG 6 cut(s) 11, 45, 146, 211, 320, 347
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 2 cut(s) 19, 129
MaeI CTAG 1 cut(s) 36
MaeII ACGT 1 cut(s) 194
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 374
MluNI TGGCCA 1 cut(s) 374
MnlI CCTC 3 cut(s) 16, 74, 249
Mox20I TGGCCA 1 cut(s) 374
Mph1103I ATGCAT 1 cut(s) 122
MscI TGGCCA 1 cut(s) 374
MseI TTAA 3 cut(s) 101, 366, 406
MslI CAYNNNNRTG 2 cut(s) 109, 251
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 3 cut(s) 182, 243, 330
NdeII GATC 1 cut(s) 82
NlaIII CATG 4 cut(s) 120, 237, 250, 373
NlaIV GGNNCC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 122
PfeI GAWTC 1 cut(s) 301
PkrI GCNGC 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 281
RsaI GTAC 2 cut(s) 191, 266
RsaNI GTAC 2 cut(s) 190, 265
RseI CAYNNNNRTG 2 cut(s) 109, 251
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 3 cut(s) 101, 366, 406
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 1 cut(s) 82
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 1 cut(s) 351
SetI ASST 4 cut(s) 95, 197, 205, 351
SfaNI GCATC 2 cut(s) 19, 129
SmiMI CAYNNNNRTG 2 cut(s) 109, 251
SsiI CCGC 2 cut(s) 244, 360
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 284
TaiI ACGT 1 cut(s) 197
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 3 cut(s) 101, 366, 406
Tru9I TTAA 3 cut(s) 101, 366, 406
TscAI CASTG 1 cut(s) 311
TseI GCWGC 1 cut(s) 173
TspDTI ATGAA 3 cut(s) 187, 222, 263
TspRI CASTG 1 cut(s) 311
XspI CTAG 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.