Rh4CG162800

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000741
Physical Location & Seq
Reverse (-)
35750741 .. 35751841
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG162800.1

Sequence Viewer

Length: 465 bp
ATGTCCTCATCAAACTTCCACAAGCAGGATGCTCCTAAAACCCCCGAGCCTTGGAAATCTGACCTCCTTGACTGCTTCTCCGATCCCAAAAGCTGTTGCTTAACATTTTGGTGTCCATGCATCACTTTTGGCCGGATTGCTGAGATTGTGGATAAAGGCTCTCCATCTTGTGCTGTAAGTGGAGCACTGTATGCGTTCATAGCTATCGTGATTGGTTTTCCTTACTGCTATTCATGCTCCTACCGCTTGAAGATGAGGGAGCAGTACGCTTTGGACGGGAACCATTGTAGTGATTTTTTGATTCACTGCTTCTGTGAGACCTGTGCCTTGTGCCAGGAGTATCGTGAGCTCCAAAGTCGCGGTTTTAACATGGCCATCGGATGGGATGGAAATATGGATGCAAGGAACCGCAAAGTGGCAATGGCCCCGGTGCCTCCGATGGTGGAGGAAGGCATGAAACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

154

Amino Acids

17.37

Weight (kDa)

6.27

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 18 - 116 1.3e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 430
AccB7I CCANNNNNTGG 1 cut(s) 381
AccII CGCG 1 cut(s) 360
AciI CCGC 3 cut(s) 244, 360, 409
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 2 cut(s) 130, 372
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 4 cut(s) 25, 51, 381, 415
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 3 cut(s) 93, 203, 349
AluI AGCT 3 cut(s) 93, 203, 349
Alw21I GWGCWC 2 cut(s) 187, 351
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 3 cut(s) 130, 372, 423
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 44
BalI TGGCCA 1 cut(s) 374
BanI GGYRCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 187, 351
BccI CCATC 5 cut(s) 172, 375, 380, 383, 433
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 335, 428
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 4 cut(s) 281, 407, 426, 432
BmrFI CCNGG 2 cut(s) 335, 428
BmsI GCATC 3 cut(s) 19, 129, 388
BpuMI CCSGG 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 50, 426
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 51, 381, 415
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 50, 426
BseGI GGATG 4 cut(s) 34, 386, 391, 403
BseLI CCNNNNNNNGG 4 cut(s) 25, 51, 381, 415
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 132, 374, 425
BshNI GGYRCC 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 187, 351
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 2 cut(s) 133, 428
BslI CCNNNNNNNGG 4 cut(s) 25, 51, 381, 415
BsmAI GTCTC 1 cut(s) 311
BsnI GGCC 3 cut(s) 132, 374, 425
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 187, 351
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 3 cut(s) 244, 360, 409
BspANI GGCC 3 cut(s) 132, 374, 425
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 4 cut(s) 281, 407, 426, 432
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 430
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 50, 426
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 4 cut(s) 34, 386, 391, 403
BstFNI CGCG 1 cut(s) 360
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 191, 200, 234, 243
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 333, 426
BstUI CGCG 1 cut(s) 360
BsuRI GGCC 3 cut(s) 132, 374, 425
BtsCI GGATG 4 cut(s) 34, 386, 391, 403
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 185, 304
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 1 cut(s) 265
CviAII CATG 4 cut(s) 117, 234, 370, 454
CviJI RGCY 8 cut(s) 49, 93, 132, 159, 203, 349, 374, 425
CviKI_1 RGCY 8 cut(s) 49, 93, 132, 159, 203, 349, 374, 425
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 1 cut(s) 141
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
EaeI YGGCCR 2 cut(s) 130, 372
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 120, 237, 373, 457
FaiI YATR 7 cut(s) 118, 192, 200, 235, 371, 395, 455
FatI CATG 4 cut(s) 116, 233, 369, 453
FokI GGATG 4 cut(s) 41, 393, 398, 410
FriOI GRGCYC 1 cut(s) 351
HaeIII GGCC 3 cut(s) 132, 374, 425
HapII CCGG 2 cut(s) 133, 428
Hin1II CATG 4 cut(s) 120, 237, 373, 457
HinfI GANTC 1 cut(s) 301
HpaII CCGG 2 cut(s) 133, 428
Hpy188I TCNGA 4 cut(s) 61, 82, 380, 438
Hpy188III TCNNGA 2 cut(s) 208, 344
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 120, 401
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 200, 234, 243
HpyF3I CTNAG 1 cut(s) 141
Hsp92II CATG 4 cut(s) 120, 237, 373, 457
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 5 cut(s) 37, 182, 242, 259, 354
LpnPI CCDG 6 cut(s) 11, 146, 320, 334, 347, 441
LweI GCATC 3 cut(s) 19, 129, 388
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 187, 351
MlsI TGGCCA 1 cut(s) 374
MluNI TGGCCA 1 cut(s) 374
MnlI CCTC 5 cut(s) 16, 74, 249, 439, 444
Mox20I TGGCCA 1 cut(s) 374
Mph1103I ATGCAT 1 cut(s) 122
MscI TGGCCA 1 cut(s) 374
MseI TTAA 2 cut(s) 101, 366
MslI CAYNNNNRTG 2 cut(s) 109, 288
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 2 cut(s) 133, 428
MspR9I CCNGG 2 cut(s) 335, 428
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 191, 200, 234, 243
NciI CCSGG 1 cut(s) 428
NdeII GATC 1 cut(s) 82
NlaIII CATG 4 cut(s) 120, 237, 373, 457
NlaIV GGNNCC 4 cut(s) 281, 407, 426, 432
NsiI ATGCAT 1 cut(s) 122
PfeI GAWTC 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 4 cut(s) 281, 407, 426, 432
PspPI GGNCC 1 cut(s) 424
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 2 cut(s) 109, 288
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 101, 366
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 335, 428
SduI GDGCHC 2 cut(s) 187, 351
SetI ASST 5 cut(s) 66, 95, 205, 323, 351
SfaNI GCATC 3 cut(s) 19, 129, 388
SmiMI CAYNNNNRTG 2 cut(s) 109, 288
SsiI CCGC 3 cut(s) 244, 360, 409
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 333, 426
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 2 cut(s) 101, 366
Tru9I TTAA 2 cut(s) 101, 366
TscAI CASTG 2 cut(s) 192, 311
TspDTI ATGAA 2 cut(s) 187, 222
TspRI CASTG 2 cut(s) 192, 311
Van91I CCANNNNNTGG 1 cut(s) 381
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.