Rorug04G0346200

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000741
Physical Location & Seq
Forward (+)
51832663 .. 51833361
699 bp
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UTR
Exon/CDS
Intron
Rorug04G0346200.1

Sequence Viewer

Length: 699 bp
ATGCCTAATAATATCAAAGCATCTCATCAATCCACACCAGTGGACTATGTTGAAACATTTCAGATTGCGGTTATGCTAAAGATGTGCATGCAATTATTAGGGCTAGTTCGGCATACTGAAAGTGAATCTAAGAGAAACAAGCTGGTCGGAGGAGATGCCAATCAAGCTCCTACTTCCTTATCGTTGGACTACAGTACACAGTCCAAAAGTATTCTTGTGAGGATAGTTCATGCAGGTGGGCAAGAGGAGCTGTTTCCGAATGCAGTTCCTGCATCTCTGCTGATGGACAAATATCCTGGGAAATGTGTTGCCCGCCCAGAAGTTTTCAAAAAGCCTCAGGAGTCCCTCTTAAGGCCAGACGAGAGCCTATTGCCTGGTCAGAAGTATTATATAATACCATGTACAACAGCACAGAAACTTAAGCGTCAACAGCAGAAGAAGGTGAAGGCCAAAGAACGTGCTGAAGATAAAGAGATGATTCTGGATGGAAAGATCACTGTGGATTCGGAAGGGGAAGACACTGATGATTCTATTTGCTCCGCCAGTGATTTCGTCTCCAAGGAAAAGAGACCAAAATCTTCAAGGAGGAGGGGAATTAGAGGAAGAAAGCCGTTTGTACCTCCCCTCCCAAAGGTCAGATCTGGTAGAGGATTTGGATGGGAGCCTAGTCTCACTTCTGTACAAGAAGTGTCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

232

Amino Acids

25.87

Weight (kDa)

9.44

Isoelectric Point (pI)

60.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 67 - 229 4.4e-17 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 224
AciI CCGC 3 cut(s) 68, 313, 540
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 4 cut(s) 196, 403, 618, 681
AfiI CCNNNNNNNGG 2 cut(s) 351, 631
AflII CTTAAG 2 cut(s) 349, 419
AgsI TTSAA 3 cut(s) 53, 328, 582
AjnI CCWGG 2 cut(s) 295, 373
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 3 cut(s) 142, 167, 250
AluI AGCT 3 cut(s) 142, 167, 250
Alw26I GTCTC 3 cut(s) 559, 562, 674
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 2 cut(s) 353, 447
Asp700I GAANNNNTTC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 454
AxyI CCTNAGG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 522
BccI CCATC 3 cut(s) 277, 479, 651
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 2 cut(s) 297, 375
BcoDI GTCTC 3 cut(s) 559, 562, 674
BfaI CTAG 2 cut(s) 104, 666
BfmI CTRYAG 1 cut(s) 190
BfrI CTTAAG 2 cut(s) 349, 419
BfuAI ACCTGC 1 cut(s) 224
BglII AGATCT 1 cut(s) 638
Bme1390I CCNGG 2 cut(s) 297, 375
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 2 cut(s) 297, 375
BmsI GCATC 3 cut(s) 29, 145, 281
BpiI GAAGAC 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 296, 558
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 631
Bse1I ACTGG 2 cut(s) 38, 543
Bse21I CCTNAGG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 2 cut(s) 297, 375
BseDI CCNNGG 2 cut(s) 296, 558
BseGI GGATG 2 cut(s) 490, 662
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 351, 631
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 2 cut(s) 38, 543
BseRI GAGGAG 3 cut(s) 165, 260, 601
BshFI GGCC 2 cut(s) 355, 449
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 351, 631
BsmAI GTCTC 3 cut(s) 559, 562, 674
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 355, 449
Bso31I GGTCTC 1 cut(s) 562
Bsp1407I TGTACA 2 cut(s) 401, 679
Bsp143I GATC 2 cut(s) 492, 638
BspACI CCGC 3 cut(s) 68, 313, 540
BspANI GGCC 2 cut(s) 355, 449
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 663
BspMI ACCTGC 1 cut(s) 224
BspTI CTTAAG 2 cut(s) 349, 419
BspTNI GGTCTC 1 cut(s) 562
BsrGI TGTACA 2 cut(s) 401, 679
BsrI ACTGG 2 cut(s) 38, 543
BssECI CCNNGG 2 cut(s) 296, 558
BssMI GATC 2 cut(s) 492, 638
BssT1I CCWWGG 1 cut(s) 558
Bst2UI CCWGG 2 cut(s) 297, 375
Bst4CI ACNGT 3 cut(s) 194, 201, 499
BstAFI CTTAAG 2 cut(s) 349, 419
BstAPI GCANNNNNTGC 1 cut(s) 269
BstAUI TGTACA 2 cut(s) 401, 679
BstC8I GCNNGC 2 cut(s) 89, 313
BstDEI CTNAG 2 cut(s) 129, 336
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 490, 662
BstKTI GATC 2 cut(s) 495, 641
BstMAI GTCTC 3 cut(s) 559, 562, 674
BstMBI GATC 2 cut(s) 492, 638
BstMWI GCNNNNNNNGC 5 cut(s) 109, 164, 247, 269, 430
BstNI CCWGG 2 cut(s) 297, 375
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 295, 373
BstSFI CTRYAG 1 cut(s) 190
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 638
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 1 cut(s) 638
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 2 cut(s) 355, 449
BtsCI GGATG 2 cut(s) 490, 662
BtsIMutI CAGTG 4 cut(s) 45, 495, 519, 550
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 89, 313
CaiI CAGNNNCTG 1 cut(s) 269
CseI GACGC 1 cut(s) 413
Csp6I GTAC 4 cut(s) 195, 402, 617, 680
CviAII CATG 4 cut(s) 88, 230, 399, 696
CviQI GTAC 4 cut(s) 195, 402, 617, 680
DdeI CTNAG 2 cut(s) 129, 336
DpnI GATC 2 cut(s) 494, 640
DpnII GATC 2 cut(s) 492, 638
EciI GGCGGA 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 558
Eco31I GGTCTC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 483
Eco81I CCTNAGG 1 cut(s) 336
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoRII CCWGG 2 cut(s) 295, 373
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 4 cut(s) 91, 233, 402, 699
FaiI YATR 9 cut(s) 48, 74, 89, 114, 231, 390, 392, 400, 697
FaqI GGGAC 1 cut(s) 328
FatI CATG 4 cut(s) 87, 229, 398, 695
FauI CCCGC 1 cut(s) 320
FokI GGATG 2 cut(s) 497, 669
FspBI CTAG 2 cut(s) 104, 666
HaeIII GGCC 2 cut(s) 355, 449
HgaI GACGC 1 cut(s) 413
Hin1II CATG 4 cut(s) 91, 233, 402, 699
HincII GTYRAC 1 cut(s) 428
HindII GTYRAC 1 cut(s) 428
HinfI GANTC 5 cut(s) 125, 341, 478, 503, 527
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 3 cut(s) 43, 197, 428
Hpy188I TCNGA 6 cut(s) 63, 149, 258, 381, 508, 638
Hpy188III TCNNGA 2 cut(s) 338, 482
Hpy8I GTNNAC 3 cut(s) 43, 197, 428
HpyAV CCTTC 3 cut(s) 433, 439, 503
HpyCH4III ACNGT 3 cut(s) 194, 201, 499
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 5 cut(s) 87, 91, 233, 263, 272
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 164, 247, 269, 430
HpyF3I CTNAG 2 cut(s) 129, 336
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 91, 233, 402, 699
Kzo9I GATC 2 cut(s) 492, 638
LmnI GCTCC 4 cut(s) 172, 247, 542, 661
LweI GCATC 3 cut(s) 29, 145, 281
MaeI CTAG 2 cut(s) 104, 666
MaeII ACGT 1 cut(s) 457
MalI GATC 2 cut(s) 494, 640
MboI GATC 2 cut(s) 492, 638
MboII GAAGA 5 cut(s) 448, 476, 527, 570, 615
MflI RGATCY 1 cut(s) 638
MluCI AATT 2 cut(s) 92, 594
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 127, 165
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 2 cut(s) 350, 420
MslI CAYNNNNRTG 2 cut(s) 38, 234
MspCI CTTAAG 2 cut(s) 349, 419
MspR9I CCNGG 2 cut(s) 297, 375
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 297, 375
MwoI GCNNNNNNNGC 5 cut(s) 109, 164, 247, 269, 430
NdeII GATC 2 cut(s) 492, 638
NlaIII CATG 4 cut(s) 91, 233, 402, 699
NlaIV GGNNCC 1 cut(s) 663
NspI RCATGY 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 38
PaeI GCATGC 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 224
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 4 cut(s) 125, 478, 503, 527
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 295, 373
PspGI CCWGG 2 cut(s) 295, 373
PspN4I GGNNCC 1 cut(s) 663
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 4 cut(s) 196, 403, 618, 681
RsaNI GTAC 4 cut(s) 195, 402, 617, 680
RseI CAYNNNNRTG 2 cut(s) 38, 234
SaqAI TTAA 2 cut(s) 350, 420
Sau3AI GATC 2 cut(s) 492, 638
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 297, 375
SetI ASST 8 cut(s) 144, 169, 238, 252, 444, 460, 622, 636
SfaNI GCATC 3 cut(s) 29, 145, 281
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 38, 234
SmlI CTYRAG 2 cut(s) 349, 419
SmoI CTYRAG 2 cut(s) 349, 419
SphI GCATGC 1 cut(s) 91
Sse9I AATT 2 cut(s) 92, 594
SsiI CCGC 3 cut(s) 68, 313, 540
SspMI CTAG 2 cut(s) 104, 666
StyD4I CCNGG 2 cut(s) 295, 373
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 3 cut(s) 194, 201, 499
TaiI ACGT 1 cut(s) 460
TasI AATT 2 cut(s) 92, 594
TatI WGTACW 3 cut(s) 194, 401, 679
TfiI GAWTC 4 cut(s) 125, 478, 503, 527
Tru1I TTAA 2 cut(s) 350, 420
Tru9I TTAA 2 cut(s) 350, 420
TscAI CASTG 4 cut(s) 45, 502, 526, 550
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 4 cut(s) 45, 502, 526, 550
Vha464I CTTAAG 2 cut(s) 349, 419
XagI CCTNNNNNAGG 1 cut(s) 629
XceI RCATGY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 2 cut(s) 104, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.