Rw4G004920

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000741
Physical Location & Seq
Forward (+)
9794742 .. 9796262
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004920.1

Sequence Viewer

Length: 453 bp
ATGTCCTCATCAAACTTCCACATGCAGGATGCTCCTAGAACCCCCGAGCCTTGGAAATCTGGCCTCCTTGACTGCTTCTCCGATCGCAAAAGCTGTTGCTTAACATGTTGGTGTCCATGCATCACTTTTGGCCGGATTGCTGAGATTGTGGATAAAGGCTCTCCATCTTGTGTTGCAAGTGGAGCACGGTACGCGTTCACAGCTTTTCCTTGCTTCTATTCATGCACCTACCGCTTGAAGATGAGGGAGCAGTACGATTTGGACGGGCACTGTTGTCGTGATTTTTTGATTCACTTCTTCTGTGAGAACTGCGCCTTGTGCCAGGAGTATCGTGAGCTCCAAAGTCGCGGTTTTAACATGGCCATCGGATGGGATGGAAATATGGATGCAAGGAACAGCGAAGTGGCAATGGCCCCGGTGCCTCCGATCGTGGAGGAAGGCATGAAACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

150

Amino Acids

17.08

Weight (kDa)

5.9

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 18 - 112 5.8e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 369
AccII CGCG 2 cut(s) 194, 348
AciI CCGC 2 cut(s) 232, 348
AcoI YGGCCR 2 cut(s) 130, 360
AfaI GTAC 2 cut(s) 191, 254
AfiI CCNNNNNNNGG 3 cut(s) 25, 51, 369
AflIII ACRYGT 2 cut(s) 104, 192
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 3 cut(s) 93, 203, 337
AluI AGCT 3 cut(s) 93, 203, 337
Alw21I GWGCWC 2 cut(s) 187, 339
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 4 cut(s) 61, 130, 360, 411
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 270
BalI TGGCCA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 187, 339
BccI CCATC 4 cut(s) 172, 363, 368, 371
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 1 cut(s) 323
BcnI CCSGG 1 cut(s) 416
BfaI CTAG 1 cut(s) 36
Bme1390I CCNGG 2 cut(s) 323, 416
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 414, 420
BmrFI CCNGG 2 cut(s) 323, 416
BmsI GCATC 3 cut(s) 19, 129, 376
BpuMI CCSGG 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 50, 414
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 51, 369
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 50, 414
BseGI GGATG 4 cut(s) 34, 374, 379, 391
BseLI CCNNNNNNNGG 3 cut(s) 25, 51, 369
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 270
Bsh1236I CGCG 2 cut(s) 194, 348
Bsh1285I CGRYCG 2 cut(s) 85, 429
BshFI GGCC 4 cut(s) 63, 132, 362, 413
BshNI GGYRCC 1 cut(s) 418
BsiEI CGRYCG 2 cut(s) 85, 429
BsiHKAI GWGCWC 2 cut(s) 187, 339
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 2 cut(s) 133, 416
BslI CCNNNNNNNGG 3 cut(s) 25, 51, 369
BsnI GGCC 4 cut(s) 63, 132, 362, 413
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 3 cut(s) 187, 270, 339
Bsp143I GATC 2 cut(s) 82, 426
BspACI CCGC 2 cut(s) 232, 348
BspANI GGCC 4 cut(s) 63, 132, 362, 413
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 2 cut(s) 194, 348
BspLI GGNNCC 2 cut(s) 414, 420
BspT107I GGYRCC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 2 cut(s) 50, 414
BssMI GATC 2 cut(s) 82, 426
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 189, 272
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 4 cut(s) 34, 374, 379, 391
BstFNI CGCG 2 cut(s) 194, 348
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 2 cut(s) 85, 429
BstMBI GATC 2 cut(s) 82, 426
BstMCI CGRYCG 2 cut(s) 85, 429
BstMWI GCNNNNNNNGC 5 cut(s) 182, 191, 200, 231, 318
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 2 cut(s) 25, 108
BstSCI CCNGG 2 cut(s) 321, 414
BstSLI GKGCMC 1 cut(s) 270
BstUI CGCG 2 cut(s) 194, 348
BsuRI GGCC 4 cut(s) 63, 132, 362, 413
BtsCI GGATG 4 cut(s) 34, 374, 379, 391
BtsIMutI CAGTG 1 cut(s) 268
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 412
Csp6I GTAC 2 cut(s) 190, 253
CviAII CATG 6 cut(s) 22, 105, 117, 222, 358, 442
CviJI RGCY 9 cut(s) 49, 63, 93, 132, 159, 203, 337, 362, 413
CviKI_1 RGCY 9 cut(s) 49, 63, 93, 132, 159, 203, 337, 362, 413
CviQI GTAC 2 cut(s) 190, 253
DdeI CTNAG 1 cut(s) 141
DpnI GATC 2 cut(s) 84, 428
DpnII GATC 2 cut(s) 82, 426
EaeI YGGCCR 2 cut(s) 130, 360
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 6 cut(s) 25, 108, 120, 225, 361, 445
FaiI YATR 7 cut(s) 23, 106, 118, 223, 359, 383, 443
FatI CATG 6 cut(s) 21, 104, 116, 221, 357, 441
FokI GGATG 4 cut(s) 41, 381, 386, 398
FriOI GRGCYC 1 cut(s) 339
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 313
HaeIII GGCC 4 cut(s) 63, 132, 362, 413
HapII CCGG 2 cut(s) 133, 416
HhaI GCGC 1 cut(s) 314
Hin1II CATG 6 cut(s) 25, 108, 120, 225, 361, 445
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HinfI GANTC 1 cut(s) 289
HpaII CCGG 2 cut(s) 133, 416
Hpy166II GTNNAC 1 cut(s) 198
Hpy188I TCNGA 3 cut(s) 82, 368, 426
Hpy188III TCNNGA 2 cut(s) 278, 332
Hpy8I GTNNAC 1 cut(s) 198
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 2 cut(s) 189, 272
HpyCH4V TGCA 5 cut(s) 25, 120, 176, 225, 389
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 191, 200, 231, 318
HpyF3I CTNAG 1 cut(s) 141
Hsp92II CATG 6 cut(s) 25, 108, 120, 225, 361, 445
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 82, 426
LmnI GCTCC 4 cut(s) 37, 182, 247, 342
LpnPI CCDG 6 cut(s) 11, 45, 146, 308, 335, 429
LweI GCATC 3 cut(s) 19, 129, 376
MaeI CTAG 1 cut(s) 36
MalI GATC 2 cut(s) 84, 428
MboI GATC 2 cut(s) 82, 426
MboII GAAGA 2 cut(s) 250, 289
MhlI GDGCHC 3 cut(s) 187, 270, 339
MlsI TGGCCA 1 cut(s) 362
MluI ACGCGT 1 cut(s) 192
MluNI TGGCCA 1 cut(s) 362
MnlI CCTC 5 cut(s) 16, 74, 237, 427, 432
Mox20I TGGCCA 1 cut(s) 362
Mph1103I ATGCAT 1 cut(s) 122
MscI TGGCCA 1 cut(s) 362
MseI TTAA 2 cut(s) 101, 354
MslI CAYNNNNRTG 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 2 cut(s) 133, 416
MspR9I CCNGG 2 cut(s) 323, 416
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 2 cut(s) 194, 348
MwoI GCNNNNNNNGC 5 cut(s) 182, 191, 200, 231, 318
NciI CCSGG 1 cut(s) 416
NdeII GATC 2 cut(s) 82, 426
NlaIII CATG 6 cut(s) 25, 108, 120, 225, 361, 445
NlaIV GGNNCC 2 cut(s) 414, 420
NsiI ATGCAT 1 cut(s) 122
NspI RCATGY 2 cut(s) 25, 108
PciI ACATGT 1 cut(s) 104
PfeI GAWTC 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 369
Ple19I CGATCG 2 cut(s) 85, 429
PscI ACATGT 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 414, 420
PspPI GGNCC 1 cut(s) 412
PvuI CGATCG 2 cut(s) 85, 429
RsaI GTAC 2 cut(s) 191, 254
RsaNI GTAC 2 cut(s) 190, 253
RseI CAYNNNNRTG 1 cut(s) 109
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 101, 354
Sau3AI GATC 2 cut(s) 82, 426
Sau96I GGNCC 1 cut(s) 412
ScrFI CCNGG 2 cut(s) 323, 416
SduI GDGCHC 3 cut(s) 187, 270, 339
SetI ASST 4 cut(s) 95, 205, 230, 339
SfaNI GCATC 3 cut(s) 19, 129, 376
SmiMI CAYNNNNRTG 1 cut(s) 109
SsiI CCGC 2 cut(s) 232, 348
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 2 cut(s) 321, 414
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 189, 272
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 2 cut(s) 101, 354
Tru9I TTAA 2 cut(s) 101, 354
TscAI CASTG 1 cut(s) 275
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 369
XceI RCATGY 2 cut(s) 25, 108
XspI CTAG 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.