pycom16g01910

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000741
Physical Location & Seq
Reverse (-)
1176940 .. 1178606
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01910.1

Sequence Viewer

Length: 627 bp
ATGTACTCGTCAAAAGCAAACGTATACGAGAAGTACTCCTCCTCCGACCACCTCCACCAACATGTCGATGCACCGGCCACGGGCATCCCAGTCAGCTCCGGTAGTGAACCTTATTTTACTACTTCTCACGAAGACATGAGCTCCCAAAGTGGCTACAGCCACATGACCCACCCACCTACCCATCTCCGCCCTCGCGCCCTCGTTCCTTGGTCCACCGGCCTCTTCGACTGCTTCTCCGATCCAAAAAACTGTTGCATAACATTTTGGTGTCCATGCATCACTTTTGGCCAAATTGCAGAGATTGTCGATAAGGGTTCTACATCTTGTGGTGCTAGTGGAGCGCTTTACACACTGATTGCTTGTGTGACGGCTTTTCCATGTATCTATTCATGCTTCTACCGCTCCAAAATGAGACAGCAATACAGCCTGGAAGAGAGCCCTTGCGGTGACTGCTTGGTTCATTGCTTCTGTGAAGGCTGCGCCTTGTGCCAAGAGTACCGAGAGCTCAAGTTTCGCGGCTTCGACATGAAACTTGGATGGCATGGAAACATCGAGGAAAGGAACCATGAAGTTGGAATGACTCCGGTGGCTCCGGTGGTGGAGGAAGGCATGAGTCGAGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

209

Amino Acids

23.22

Weight (kDa)

5.76

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 70 - 168 1.3e-36 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 402
AccI GTMKAC 1 cut(s) 24
AccII CGCG 2 cut(s) 195, 516
AciI CCGC 4 cut(s) 187, 400, 444, 516
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 2 cut(s) 75, 286
AfaI GTAC 3 cut(s) 5, 35, 497
AfeI AGCGCT 1 cut(s) 342
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 61
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 3 cut(s) 96, 141, 505
AluI AGCT 3 cut(s) 96, 141, 505
Alw21I GWGCWC 2 cut(s) 143, 507
Alw26I GTCTC 1 cut(s) 406
AlwI GGATC 1 cut(s) 233
Aor51HI AGCGCT 1 cut(s) 342
AoxI GGCC 3 cut(s) 75, 217, 286
ApeKI GCWGC 1 cut(s) 477
AspLEI GCGC 3 cut(s) 197, 343, 482
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 458
AvaII GGWCC 1 cut(s) 210
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 3 cut(s) 143, 440, 507
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 143, 507
BbvI GCAGC 1 cut(s) 464
BccI CCATC 2 cut(s) 189, 531
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 406
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 154
BfoI RGCGCY 1 cut(s) 344
BisI GCNGC 2 cut(s) 478, 517
BlsI GCNGC 2 cut(s) 479, 518
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 2 cut(s) 563, 591
BmrFI CCNGG 1 cut(s) 428
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 3 cut(s) 58, 93, 285
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpuEI CTTGAG 1 cut(s) 491
BsaJI CCNNGG 2 cut(s) 78, 206
BsaWI WCCGGW 3 cut(s) 98, 583, 592
BsaXI ACNNNNNCTCC 6 cut(s) 26, 56, 125, 155, 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 2 cut(s) 73, 215
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 2 cut(s) 78, 206
BseGI GGATG 2 cut(s) 84, 542
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 460
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 28, 31
BseXI GCAGC 1 cut(s) 464
Bsh1236I CGCG 2 cut(s) 195, 516
BshFI GGCC 3 cut(s) 77, 219, 288
BsiHKAI GWGCWC 2 cut(s) 143, 507
BsiSI CCGG 5 cut(s) 74, 99, 216, 584, 593
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 406
BsnI GGCC 3 cut(s) 77, 219, 288
Bsp1286I GDGCHC 3 cut(s) 143, 440, 507
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 4 cut(s) 187, 400, 444, 516
BspANI GGCC 3 cut(s) 77, 219, 288
BspFNI CGCG 2 cut(s) 195, 516
BspLI GGNNCC 2 cut(s) 563, 591
BspPI GGATC 1 cut(s) 233
BsrBI CCGCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 73, 215
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 2 cut(s) 73, 215
BssECI CCNNGG 2 cut(s) 78, 206
BssMI GATC 1 cut(s) 238
BssNAI GTATAC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 206
Bst1107I GTATAC 1 cut(s) 25
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 2 cut(s) 227, 426
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 84, 542
BstFNI CGCG 2 cut(s) 195, 516
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 3 cut(s) 197, 343, 482
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 4 cut(s) 338, 399, 450, 486
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 2 cut(s) 195, 516
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 572
BstZ17I GTATAC 1 cut(s) 25
BsuRI GGCC 3 cut(s) 77, 219, 288
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 2 cut(s) 84, 542
BtsIMutI CAGTG 1 cut(s) 350
CfoI GCGC 3 cut(s) 197, 343, 482
Cfr10I RCCGGY 2 cut(s) 73, 215
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 3 cut(s) 4, 34, 496
CviQI GTAC 3 cut(s) 4, 34, 496
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
EaeI YGGCCR 2 cut(s) 75, 286
Eam1104I CTCTTC 2 cut(s) 227, 426
EarI CTCTTC 2 cut(s) 227, 426
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 2 cut(s) 141, 505
Eco130I CCWWGG 1 cut(s) 206
Eco24I GRGCYC 3 cut(s) 143, 440, 507
Eco47I GGWCC 1 cut(s) 210
Eco47III AGCGCT 1 cut(s) 342
Eco53kI GAGCTC 2 cut(s) 141, 505
EcoICRI GAGCTC 2 cut(s) 141, 505
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 278
EcoT38I GRGCYC 3 cut(s) 143, 440, 507
ErhI CCWWGG 1 cut(s) 206
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 478, 517
FokI GGATG 2 cut(s) 71, 549
FriOI GRGCYC 3 cut(s) 143, 440, 507
Fsp4HI GCNGC 2 cut(s) 478, 517
FspBI CTAG 1 cut(s) 333
GlaI GCGC 3 cut(s) 196, 342, 481
GluI GCNGC 2 cut(s) 478, 517
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 3 cut(s) 77, 219, 288
HapII CCGG 5 cut(s) 74, 99, 216, 584, 593
HhaI GCGC 3 cut(s) 197, 343, 482
Hin6I GCGC 3 cut(s) 195, 341, 480
HinP1I GCGC 3 cut(s) 195, 341, 480
HinfI GANTC 2 cut(s) 580, 613
HpaII CCGG 5 cut(s) 74, 99, 216, 584, 593
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 3 cut(s) 25, 107, 213
Hpy188I TCNGA 2 cut(s) 46, 238
Hpy188III TCNNGA 2 cut(s) 128, 617
Hpy8I GTNNAC 3 cut(s) 25, 107, 213
HpyAV CCTTC 2 cut(s) 467, 599
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 4 cut(s) 71, 255, 276, 296
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 399, 450, 486
HpySE526I ACGT 1 cut(s) 21
HspAI GCGC 3 cut(s) 195, 341, 480
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 5 cut(s) 101, 146, 338, 407, 595
LpnPI CCDG 8 cut(s) 87, 102, 112, 229, 413, 440, 597, 606
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 3 cut(s) 58, 93, 285
MaeI CTAG 1 cut(s) 333
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 364, 446
MalI GATC 1 cut(s) 240
MbiI CCGCTC 1 cut(s) 402
MboI GATC 1 cut(s) 238
MboII GAAGA 3 cut(s) 143, 214, 443
MhlI GDGCHC 3 cut(s) 143, 440, 507
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 1 cut(s) 291
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 2 cut(s) 574, 622
MmeI TCCRAC 2 cut(s) 69, 553
MnlI CCTC 8 cut(s) 49, 52, 62, 201, 209, 230, 547, 595
Mox20I TGGCCA 1 cut(s) 288
Mph1103I ATGCAT 1 cut(s) 278
MscI TGGCCA 1 cut(s) 288
MslI CAYNNNNRTG 3 cut(s) 60, 66, 265
Msp20I TGGCCA 1 cut(s) 288
MspI CCGG 5 cut(s) 74, 99, 216, 584, 593
MspR9I CCNGG 1 cut(s) 428
MvaI CCWGG 1 cut(s) 428
MvnI CGCG 2 cut(s) 195, 516
MwoI GCNNNNNNNGC 4 cut(s) 338, 399, 450, 486
NdeII GATC 1 cut(s) 238
NlaIV GGNNCC 2 cut(s) 563, 591
NmuCI GTSAC 2 cut(s) 364, 446
NsiI ATGCAT 1 cut(s) 278
NspI RCATGY 1 cut(s) 65
PciI ACATGT 1 cut(s) 61
PkrI GCNGC 2 cut(s) 479, 518
PleI GAGTC 2 cut(s) 574, 621
PpsI GAGTC 2 cut(s) 574, 621
PscI ACATGT 1 cut(s) 61
Psp124BI GAGCTC 2 cut(s) 143, 507
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 563, 591
PspPI GGNCC 1 cut(s) 210
RsaI GTAC 3 cut(s) 5, 35, 497
RsaNI GTAC 3 cut(s) 4, 34, 496
RseI CAYNNNNRTG 3 cut(s) 60, 66, 265
SacI GAGCTC 2 cut(s) 143, 507
SatI GCNGC 2 cut(s) 478, 517
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 210
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 2 cut(s) 574, 622
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 3 cut(s) 143, 440, 507
SetI ASST 7 cut(s) 24, 54, 98, 112, 143, 178, 507
SfaNI GCATC 3 cut(s) 58, 93, 285
SfcI CTRYAG 1 cut(s) 154
SinI GGWCC 1 cut(s) 210
SmiMI CAYNNNNRTG 3 cut(s) 60, 66, 265
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 1 cut(s) 291
SsiI CCGC 4 cut(s) 187, 400, 444, 516
SspMI CTAG 1 cut(s) 333
SstI GAGCTC 2 cut(s) 143, 507
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 24
TaqI TCGA 6 cut(s) 66, 225, 306, 522, 552, 616
TasI AATT 1 cut(s) 291
TatI WGTACW 2 cut(s) 3, 33
TauI GCSGC 1 cut(s) 519
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 2 cut(s) 364, 446
TseI GCWGC 1 cut(s) 477
Tsp45I GTSAC 2 cut(s) 364, 446
TspDTI ATGAA 4 cut(s) 378, 449, 542, 582
TspRI CASTG 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 210
XceI RCATGY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.