Rw4G034620

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000741
Physical Location & Seq
Reverse (-)
61635880 .. 61637095
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034620.1

Sequence Viewer

Length: 603 bp
ATGTACTCGTCAAAGCCGAAATCGTACGAGAAGTTCTCGGAGTCCAACACCGATGCTGCTTCTCCGCCGACGGGTATTCCGGTCAGCTCCAGCAACCCTTACTTCAGTCATGATGATCATGAAAACAGCTCCCAAATGCCGGTTGCTCCGCCATACCACCTCCGCCCTAGAGCCCCCGAGCCGTGGAAGACCGGCCTCTGCGACTGCCTCTCGGATCCCAAAAACTGCTGCACAACATTTTGGTGCCCTTGTATCACTTTCGGCCAGATTGCAGAGATTGTGGATAAGGGCTCTACTTCTTGTGGTGCAAGTGGAGCACTATACACTCTGATATTTTGTGTGACTGGTTGTCCTTGCTTCTATTCATGCTTCTACCGTTCCAAAATGAGGCAGCAATACACTCTTGATGAGAGCCCTTGTGGTGATTGCTTGGTTCATTGCTTCTGCGAGTACTGCGCCCTGTGCCAGGAGTACCGTGAGCTTCAAAATCGCGGTTTCGACATGGTTATCGGATGGCATGGAAATATCGAGGAAAGGAACCGTGAAGTGGCAATGAATCCAGTGCCCCCGGTCATGGAGGAAGGCATGAGTCGAGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

200

Amino Acids

22.49

Weight (kDa)

5.08

Isoelectric Point (pI)

62.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 62 - 160 4.2e-35 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccII CGCG 1 cut(s) 492
AciI CCGC 4 cut(s) 65, 149, 163, 492
AclWI GGATC 2 cut(s) 209, 222
AcoI YGGCCR 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 88
AfaI GTAC 4 cut(s) 5, 26, 452, 473
AfiI CCNNNNNNNGG 7 cut(s) 71, 139, 183, 387, 466, 547, 574
AgsI TTSAA 1 cut(s) 485
AhdI GACNNNNNGTC 1 cut(s) 348
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 3 cut(s) 87, 129, 481
AluI AGCT 3 cut(s) 87, 129, 481
Alw21I GWGCWC 1 cut(s) 319
AlwI GGATC 2 cut(s) 209, 222
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 193, 262
ApeKI GCWGC 3 cut(s) 56, 228, 391
AspLEI GCGC 1 cut(s) 458
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 176
BaeGI GKGCMC 2 cut(s) 248, 567
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 243
BanII GRGCYC 3 cut(s) 175, 293, 416
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 43, 215, 403
BccI CCATC 1 cut(s) 507
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 467
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 1 cut(s) 569
BfaI CTAG 1 cut(s) 168
BisI GCNGC 3 cut(s) 57, 229, 392
BlsI GCNGC 3 cut(s) 58, 230, 393
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 467, 569
BmeRI GACNNNNNGTC 1 cut(s) 348
BmeT110I CYCGRG 1 cut(s) 176
BmiI GGNNCC 3 cut(s) 216, 245, 539
BmrFI CCNGG 2 cut(s) 467, 569
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 569
BsaJI CCNNGG 2 cut(s) 182, 567
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 139, 183, 387, 466, 547, 574
Bse118I RCCGGY 2 cut(s) 139, 191
Bse1I ACTGG 2 cut(s) 349, 560
Bse3DI GCAATG 2 cut(s) 436, 558
BseBI CCWGG 1 cut(s) 467
BseDI CCNNGG 2 cut(s) 182, 567
BseGI GGATG 1 cut(s) 518
BseLI CCNNNNNNNGG 7 cut(s) 71, 139, 183, 387, 466, 547, 574
BseMI GCAATG 2 cut(s) 436, 558
BseNI ACTGG 2 cut(s) 349, 560
BseSI GKGCMC 2 cut(s) 248, 567
BseXI GCAGC 3 cut(s) 43, 215, 403
BsgI GTGCAG 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 2 cut(s) 195, 264
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 4 cut(s) 80, 140, 192, 569
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 7 cut(s) 71, 139, 183, 387, 466, 547, 574
BsnI GGCC 2 cut(s) 195, 264
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 6 cut(s) 175, 248, 293, 319, 416, 567
Bsp143I GATC 3 cut(s) 115, 214, 595
BspACI CCGC 4 cut(s) 65, 149, 163, 492
BspANI GGCC 2 cut(s) 195, 264
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 2 cut(s) 109, 118
BspLI GGNNCC 3 cut(s) 216, 245, 539
BspPI GGATC 2 cut(s) 209, 222
BspT107I GGYRCC 1 cut(s) 243
BsrDI GCAATG 2 cut(s) 436, 558
BsrFI RCCGGY 2 cut(s) 139, 191
BsrI ACTGG 2 cut(s) 349, 560
BssAI RCCGGY 2 cut(s) 139, 191
BssECI CCNNGG 2 cut(s) 182, 567
BssMI GATC 3 cut(s) 115, 214, 595
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 3 cut(s) 377, 476, 542
BstDSI CCRYGG 1 cut(s) 182
BstENI CCTNNNNNAGG 1 cut(s) 464
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 3 cut(s) 118, 217, 598
BstMBI GATC 3 cut(s) 115, 214, 595
BstMWI GCNNNNNNNGC 3 cut(s) 314, 453, 462
BstNI CCWGG 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 465, 567
BstSLI GKGCMC 2 cut(s) 248, 567
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 43, 215, 403
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 2 cut(s) 195, 264
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 1 cut(s) 518
BtsIMutI CAGTG 1 cut(s) 567
CciI TCATGA 2 cut(s) 109, 118
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 2 cut(s) 139, 191
Csp6I GTAC 4 cut(s) 4, 25, 451, 472
CviAII CATG 7 cut(s) 110, 119, 366, 502, 518, 574, 586
CviQI GTAC 4 cut(s) 4, 25, 451, 472
DpnI GATC 3 cut(s) 117, 216, 597
DpnII GATC 3 cut(s) 115, 214, 595
DriI GACNNNNNGTC 1 cut(s) 348
EaeI YGGCCR 1 cut(s) 262
Eam1105I GACNNNNNGTC 1 cut(s) 348
EciI GGCGGA 3 cut(s) 54, 138, 152
Eco24I GRGCYC 3 cut(s) 175, 293, 416
Eco57I CTGAAG 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 465
EcoT38I GRGCYC 3 cut(s) 175, 293, 416
FaeI CATG 7 cut(s) 113, 122, 369, 505, 521, 577, 589
FaiI YATR 9 cut(s) 111, 120, 154, 322, 367, 503, 519, 575, 587
FatI CATG 7 cut(s) 109, 118, 365, 501, 517, 573, 585
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 57, 229, 392
FokI GGATG 1 cut(s) 525
FriOI GRGCYC 3 cut(s) 175, 293, 416
Fsp4HI GCNGC 3 cut(s) 57, 229, 392
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 457
GluI GCNGC 3 cut(s) 57, 229, 392
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 2 cut(s) 195, 264
HapII CCGG 4 cut(s) 80, 140, 192, 569
HhaI GCGC 1 cut(s) 458
Hin1II CATG 7 cut(s) 113, 122, 369, 505, 521, 577, 589
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 3 cut(s) 41, 556, 589
HpaII CCGG 4 cut(s) 80, 140, 192, 569
HphI GGTGA 1 cut(s) 434
Hpy188I TCNGA 4 cut(s) 40, 214, 330, 512
Hpy188III TCNNGA 4 cut(s) 110, 119, 404, 593
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 3 cut(s) 377, 476, 542
HpyCH4V TGCA 3 cut(s) 231, 272, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 453, 462
Hsp92II CATG 7 cut(s) 113, 122, 369, 505, 521, 577, 589
HspAI GCGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 115, 214, 595
LmnI GCTCC 4 cut(s) 92, 134, 151, 314
Lsp1109I GCAGC 3 cut(s) 43, 215, 403
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 117, 216, 597
MboI GATC 3 cut(s) 115, 214, 595
MboII GAAGA 1 cut(s) 199
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 6 cut(s) 175, 248, 293, 319, 416, 567
MlyI GAGTC 2 cut(s) 50, 598
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 6 cut(s) 170, 206, 218, 381, 523, 571
MslI CAYNNNNRTG 1 cut(s) 241
MspI CCGG 4 cut(s) 80, 140, 192, 569
MspR9I CCNGG 2 cut(s) 467, 569
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 3 cut(s) 314, 453, 462
NciI CCSGG 1 cut(s) 569
NdeII GATC 3 cut(s) 115, 214, 595
NlaIII CATG 7 cut(s) 113, 122, 369, 505, 521, 577, 589
NlaIV GGNNCC 3 cut(s) 216, 245, 539
NmuCI GTSAC 1 cut(s) 340
PagI TCATGA 2 cut(s) 109, 118
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 556
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 3 cut(s) 58, 230, 393
PleI GAGTC 2 cut(s) 49, 597
PpsI GAGTC 2 cut(s) 49, 597
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 216, 245, 539
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 4 cut(s) 5, 26, 452, 473
RsaNI GTAC 4 cut(s) 4, 25, 451, 472
RseI CAYNNNNRTG 1 cut(s) 241
SatI GCNGC 3 cut(s) 57, 229, 392
Sau3AI GATC 3 cut(s) 115, 214, 595
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 2 cut(s) 50, 598
ScrFI CCNGG 2 cut(s) 467, 569
SduI GDGCHC 6 cut(s) 175, 248, 293, 319, 416, 567
SetI ASST 4 cut(s) 89, 131, 162, 483
SfaNI GCATC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 241
SsiI CCGC 4 cut(s) 65, 149, 163, 492
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 465, 567
TaaI ACNGT 3 cut(s) 377, 476, 542
TaqI TCGA 3 cut(s) 498, 528, 592
TatI WGTACW 2 cut(s) 3, 450
TfiI GAWTC 1 cut(s) 556
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 3 cut(s) 56, 228, 391
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 4 cut(s) 135, 354, 425, 569
TspRI CASTG 1 cut(s) 567
XagI CCTNNNNNAGG 1 cut(s) 464
XspI CTAG 1 cut(s) 168
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.