FvH4_4g33890

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000741
Physical Location & Seq
Reverse (-)
31930698 .. 31932404
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33890.t1

Sequence Viewer

Length: 615 bp
ATGTACTCGTCAAAGCCGAATTCGAAAGAGAAGTTTTCGGATACGTCGAACACCGATGCTTCTTCTCCGCCGACGGGTGTTCCGGTCAGCTCCAGCAACCCTTACTTCAGTAGTAGTCAAGATGATCATGACAACAACAGCTCCCAAATGCCGGCTGCTCCGCCATACCATCTCCGCCCTAGAGCCCCCGAGCCGTGGAAGACCGGCCTCTTCGACTGCTTCAACGATCCCAAAAACTGCTGCACAACGTTCTGGTGCCCTTGCATCACTTTCGGCCAGATTGCAGAGATTGTGGACAAGGGCTCTACTTCTTGTGGTGCAAGTGGAGCACTATACACACTGATATTTTGTGTGACTGGTTGTCCTTGCTTCTATTCATGCTTCTACCGCTCCAAAATGAGGCAGCAGTACACACTTGATGCGAGCCCTTGTGGTGATTGCCTGGTTCATTGCTTCTGCGAGTACTGCGCCCTGTGCCAGGAGTACCGTGAGCTGCAAAATCGCGGTTTCGACATGGTCATCGGATGGCATGGAAATATCGAGGAAAGGAACCGTGAAGTGGCGATGAATCCAGTGCCGCCGGTCATGGAGGAAGGCATGAGTCGAGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

205

Amino Acids

22.81

Weight (kDa)

5.18

Isoelectric Point (pI)

63.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 66 - 164 2.9e-35 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 390
AccII CGCG 1 cut(s) 504
AciI CCGC 6 cut(s) 68, 161, 175, 388, 504, 578
AclI AACGTT 1 cut(s) 248
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 91
AfaI GTAC 4 cut(s) 5, 410, 464, 485
AfiI CCNNNNNNNGG 6 cut(s) 74, 151, 195, 399, 478, 559
AgsI TTSAA 1 cut(s) 223
AhdI GACNNNNNGTC 1 cut(s) 360
AjnI CCWGG 2 cut(s) 441, 477
AluBI AGCT 3 cut(s) 90, 141, 493
AluI AGCT 3 cut(s) 90, 141, 493
Alw21I GWGCWC 1 cut(s) 331
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 205, 274
ApeKI GCWGC 4 cut(s) 155, 240, 403, 493
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 260
BanI GGYRCC 1 cut(s) 255
BanII GRGCYC 3 cut(s) 187, 305, 428
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 142, 227, 415, 480
BccI CCATC 2 cut(s) 177, 519
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 2 cut(s) 443, 479
BciVI GTATCC 1 cut(s) 34
BclI TGATCA 1 cut(s) 124
BfaI CTAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 34
BisI GCNGC 5 cut(s) 156, 241, 404, 494, 578
BlsI GCNGC 5 cut(s) 157, 242, 405, 495, 579
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 2 cut(s) 443, 479
BmeRI GACNNNNNGTC 1 cut(s) 360
BmeT110I CYCGRG 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 257, 551
BmrFI CCNGG 2 cut(s) 443, 479
BmsI GCATC 3 cut(s) 46, 273, 409
BpiI GAAGAC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 194
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 6 cut(s) 74, 151, 195, 399, 478, 559
Bse118I RCCGGY 3 cut(s) 151, 203, 580
Bse1I ACTGG 2 cut(s) 361, 572
Bse3DI GCAATG 1 cut(s) 448
BseBI CCWGG 2 cut(s) 443, 479
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 530
BseLI CCNNNNNNNGG 6 cut(s) 74, 151, 195, 399, 478, 559
BseMI GCAATG 1 cut(s) 448
BseNI ACTGG 2 cut(s) 361, 572
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 4 cut(s) 142, 227, 415, 480
BsgI GTGCAG 1 cut(s) 226
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 207, 276
BshNI GGYRCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 83, 152, 204, 581
BslI CCNNNNNNNGG 6 cut(s) 74, 151, 195, 399, 478, 559
BsnI GGCC 2 cut(s) 207, 276
BsoBI CYCGRG 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 5 cut(s) 187, 260, 305, 331, 428
Bsp143I GATC 2 cut(s) 124, 226
BspACI CCGC 6 cut(s) 68, 161, 175, 388, 504, 578
BspANI GGCC 2 cut(s) 207, 276
BspFNI CGCG 1 cut(s) 504
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 257, 551
BspPI GGATC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 448
BsrFI RCCGGY 3 cut(s) 151, 203, 580
BsrI ACTGG 2 cut(s) 361, 572
BssAI RCCGGY 3 cut(s) 151, 203, 580
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 2 cut(s) 124, 226
Bst2UI CCWGG 2 cut(s) 443, 479
Bst4CI ACNGT 2 cut(s) 488, 554
Bst6I CTCTTC 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 153, 424
BstDSI CCRYGG 1 cut(s) 194
BstENI CCTNNNNNAGG 1 cut(s) 476
BstF5I GGATG 1 cut(s) 530
BstFNI CGCG 1 cut(s) 504
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 2 cut(s) 127, 229
BstMBI GATC 2 cut(s) 124, 226
BstMWI GCNNNNNNNGC 4 cut(s) 326, 387, 465, 474
BstNI CCWGG 2 cut(s) 443, 479
BstSCI CCNGG 2 cut(s) 441, 477
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 4 cut(s) 142, 227, 415, 480
BstV2I GAAGAC 1 cut(s) 206
BsuI GTATCC 1 cut(s) 34
BsuRI GGCC 2 cut(s) 207, 276
BtgI CCRYGG 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 578
BtsCI GGATG 1 cut(s) 530
BtsIMutI CAGTG 2 cut(s) 338, 579
Cac8I GCNNGC 2 cut(s) 153, 424
CciI TCATGA 1 cut(s) 127
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 3 cut(s) 151, 203, 580
Csp6I GTAC 4 cut(s) 4, 409, 463, 484
CviAII CATG 6 cut(s) 128, 378, 514, 530, 586, 598
CviQI GTAC 4 cut(s) 4, 409, 463, 484
DpnI GATC 2 cut(s) 126, 228
DpnII GATC 2 cut(s) 124, 226
DriI GACNNNNNGTC 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 215
Eam1105I GACNNNNNGTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 215
EciI GGCGGA 3 cut(s) 57, 150, 164
Eco24I GRGCYC 3 cut(s) 187, 305, 428
Eco57I CTGAAG 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 2 cut(s) 441, 477
EcoT38I GRGCYC 3 cut(s) 187, 305, 428
FaeI CATG 6 cut(s) 131, 381, 517, 533, 589, 601
FaiI YATR 8 cut(s) 129, 166, 334, 379, 515, 531, 587, 599
FatI CATG 6 cut(s) 127, 377, 513, 529, 585, 597
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 5 cut(s) 156, 241, 404, 494, 578
FokI GGATG 1 cut(s) 537
FriOI GRGCYC 3 cut(s) 187, 305, 428
Fsp4HI GCNGC 5 cut(s) 156, 241, 404, 494, 578
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 469
GluI GCNGC 5 cut(s) 156, 241, 404, 494, 578
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 2 cut(s) 207, 276
HapII CCGG 4 cut(s) 83, 152, 204, 581
HhaI GCGC 1 cut(s) 470
Hin1II CATG 6 cut(s) 131, 381, 517, 533, 589, 601
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HinfI GANTC 2 cut(s) 568, 601
HpaII CCGG 4 cut(s) 83, 152, 204, 581
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 295, 411
Hpy188I TCNGA 2 cut(s) 40, 524
Hpy188III TCNNGA 3 cut(s) 119, 128, 605
Hpy8I GTNNAC 2 cut(s) 295, 411
Hpy99I CGWCG 2 cut(s) 49, 76
HpyAV CCTTC 1 cut(s) 587
HpyCH4III ACNGT 2 cut(s) 488, 554
HpyCH4IV ACGT 2 cut(s) 44, 248
HpyCH4V TGCA 5 cut(s) 243, 264, 284, 320, 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 387, 465, 474
HpySE526I ACGT 2 cut(s) 44, 248
Hsp92II CATG 6 cut(s) 131, 381, 517, 533, 589, 601
HspAI GCGC 1 cut(s) 468
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 2 cut(s) 124, 226
LmnI GCTCC 5 cut(s) 95, 146, 163, 326, 395
Lsp1109I GCAGC 4 cut(s) 142, 227, 415, 480
LweI GCATC 3 cut(s) 46, 273, 409
MaeI CTAG 1 cut(s) 180
MaeII ACGT 2 cut(s) 44, 248
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 126, 228
MbiI CCGCTC 1 cut(s) 390
MboI GATC 2 cut(s) 124, 226
MboII GAAGA 3 cut(s) 54, 202, 211
MhlI GDGCHC 5 cut(s) 187, 260, 305, 331, 428
MluCI AATT 1 cut(s) 19
MlyI GAGTC 1 cut(s) 610
MnlI CCTC 4 cut(s) 218, 393, 535, 583
MroNI GCCGGC 1 cut(s) 151
MspI CCGG 4 cut(s) 83, 152, 204, 581
MspR9I CCNGG 2 cut(s) 443, 479
MvaI CCWGG 2 cut(s) 443, 479
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 4 cut(s) 326, 387, 465, 474
NaeI GCCGGC 1 cut(s) 153
NdeII GATC 2 cut(s) 124, 226
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 6 cut(s) 131, 381, 517, 533, 589, 601
NlaIV GGNNCC 2 cut(s) 257, 551
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 23
PagI TCATGA 1 cut(s) 127
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 44
PdiI GCCGGC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 568
PflFI GACNNNGTC 1 cut(s) 515
PkrI GCNGC 5 cut(s) 157, 242, 405, 495, 579
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Psp1406I AACGTT 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 441, 477
PspGI CCWGG 2 cut(s) 441, 477
PspN4I GGNNCC 2 cut(s) 257, 551
PsyI GACNNNGTC 1 cut(s) 515
RsaI GTAC 4 cut(s) 5, 410, 464, 485
RsaNI GTAC 4 cut(s) 4, 409, 463, 484
SatI GCNGC 5 cut(s) 156, 241, 404, 494, 578
Sau3AI GATC 2 cut(s) 124, 226
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 2 cut(s) 443, 479
SduI GDGCHC 5 cut(s) 187, 260, 305, 331, 428
SetI ASST 5 cut(s) 47, 92, 143, 251, 495
SfaNI GCATC 3 cut(s) 46, 273, 409
SfuI TTCGAA 1 cut(s) 23
Sse9I AATT 1 cut(s) 19
SsiI CCGC 6 cut(s) 68, 161, 175, 388, 504, 578
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 441, 477
TaaI ACNGT 2 cut(s) 488, 554
TaiI ACGT 2 cut(s) 47, 251
TaqI TCGA 6 cut(s) 23, 47, 213, 510, 540, 604
TasI AATT 1 cut(s) 19
TatI WGTACW 3 cut(s) 3, 408, 462
TauI GCSGC 1 cut(s) 580
TfiI GAWTC 1 cut(s) 568
TscAI CASTG 2 cut(s) 345, 579
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 4 cut(s) 155, 240, 403, 493
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 3 cut(s) 366, 437, 581
TspRI CASTG 2 cut(s) 345, 579
Tth111I GACNNNGTC 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 476
XapI RAATTY 1 cut(s) 19
XspI CTAG 1 cut(s) 180
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.