Rh4AG401700

Protein PLANT CADMIUM RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000741
Physical Location & Seq
Reverse (-)
70427623 .. 70429191
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG401700.1

Sequence Viewer

Length: 600 bp
ATGTCCTCATCAAAACCGAATTCTTACGAAAAGTTCACAGATGCTCCTCCGGCGACGGGTATTCCGGTGAGCTCCAGCAACCCCTACTTTAGTGGAGCTCATGATCACCACCATGATCAAGATAACTCCCGCATGCCGGATGCTCCTCCATATCATCTCCATGTTAGAACCCCCGAGCCTTGGAAATCTGGCCTCCTTGACTGCTTCTCGGATCCTAAAAGCTGTTGCATAACATTTTGGTGTCCATGCATCACCTTTGGCCAGATTGCTGAGATTGTGGATAAAGGCTCTATATCTTGTGGGGCAAGTGGAGCACTGTACACCTTGATAGCTTTTGTGACTGGTTGTTCTTGCATCTATTCATGCTTCTACCGCTCCAAACTGAGGCAGCAATACACTTTGGAAGAGAGTCCGTGTGGTGATTGTTTGGTTCACTGCTTCTGCGAACCCTGCGCCTTGTGCCAGGAATACCGTGAGCTGCAAAGTCGCGGTTTTAATATGGCCATCGGATGGGATGGAAATATGGATGCAAGGAACCACGAAGTGGCAATGGCCCCGGTGCCCCCGATGGTGGAGGAAGGCATGAGTCGTGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

199

Amino Acids

22.07

Weight (kDa)

5.39

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 61 - 159 1.8e-36 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 559
AccB7I CCANNNNNTGG 2 cut(s) 510, 544
AccBSI CCGCTC 1 cut(s) 375
AccII CGCG 1 cut(s) 489
AciI CCGC 3 cut(s) 130, 373, 489
AclWI GGATC 2 cut(s) 206, 219
AcoI YGGCCR 2 cut(s) 259, 501
AcsI RAATTY 1 cut(s) 19
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 7 cut(s) 56, 136, 180, 384, 510, 544, 571
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 5 cut(s) 72, 98, 222, 332, 478
AluI AGCT 5 cut(s) 72, 98, 222, 332, 478
Alw21I GWGCWC 3 cut(s) 74, 100, 316
AlwI GGATC 2 cut(s) 206, 219
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 4 cut(s) 190, 259, 501, 552
ApeKI GCWGC 2 cut(s) 388, 478
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 553
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 4 cut(s) 79, 98, 244, 431
AvaI CYCGRG 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 564
BalI TGGCCA 2 cut(s) 261, 503
BamHI GGATCC 1 cut(s) 211
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 2 cut(s) 74, 100
Bbv12I GWGCWC 3 cut(s) 74, 100, 316
BbvI GCAGC 2 cut(s) 400, 465
BccI CCATC 4 cut(s) 504, 509, 512, 562
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 2 cut(s) 103, 115
BcnI CCSGG 1 cut(s) 557
BisI GCNGC 2 cut(s) 389, 479
BlsI GCNGC 2 cut(s) 390, 480
Bme1390I CCNGG 2 cut(s) 464, 557
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 4 cut(s) 213, 536, 555, 561
BmrFI CCNGG 2 cut(s) 464, 557
BmsI GCATC 5 cut(s) 31, 130, 258, 363, 517
BpmI CTGGAG 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 179, 555
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 7 cut(s) 56, 136, 180, 384, 510, 544, 571
Bse1I ACTGG 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 555
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 179, 555
BseGI GGATG 4 cut(s) 145, 515, 520, 532
BseLI CCNNNNNNNGG 7 cut(s) 56, 136, 180, 384, 510, 544, 571
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 2 cut(s) 261, 374
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 2 cut(s) 36, 135
BseSI GKGCMC 1 cut(s) 564
BseXI GCAGC 2 cut(s) 400, 465
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 4 cut(s) 192, 261, 503, 554
BshNI GGYRCC 1 cut(s) 559
BsiHKAI GWGCWC 3 cut(s) 74, 100, 316
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 4 cut(s) 50, 65, 137, 557
BslI CCNNNNNNNGG 7 cut(s) 56, 136, 180, 384, 510, 544, 571
BsnI GGCC 4 cut(s) 192, 261, 503, 554
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 4 cut(s) 74, 100, 316, 564
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 3 cut(s) 103, 115, 211
BspACI CCGC 3 cut(s) 130, 373, 489
BspANI GGCC 4 cut(s) 192, 261, 503, 554
BspCNI CTCAG 2 cut(s) 262, 375
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 213, 536, 555, 561
BspPI GGATC 2 cut(s) 206, 219
BspT107I GGYRCC 1 cut(s) 559
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 555
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 179, 555
BssMI GATC 3 cut(s) 103, 115, 211
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 318, 473
Bst6I CTCTTC 1 cut(s) 399
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 134
BstDEI CTNAG 2 cut(s) 270, 383
BstF5I GGATG 4 cut(s) 145, 515, 520, 532
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 3 cut(s) 106, 118, 214
BstMBI GATC 3 cut(s) 103, 115, 211
BstMWI GCNNNNNNNGC 5 cut(s) 50, 311, 372, 450, 459
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 462, 555
BstSLI GKGCMC 1 cut(s) 564
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 400, 465
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 4 cut(s) 192, 261, 503, 554
BtsCI GGATG 4 cut(s) 145, 515, 520, 532
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 314, 433
Cac8I GCNNGC 1 cut(s) 134
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 553
Csp6I GTAC 1 cut(s) 319
CviAII CATG 7 cut(s) 101, 113, 133, 161, 246, 363, 583
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 2 cut(s) 270, 383
DpnI GATC 3 cut(s) 105, 117, 213
DpnII GATC 3 cut(s) 103, 115, 211
DraIII CACNNNGTG 1 cut(s) 544
EaeI YGGCCR 2 cut(s) 259, 501
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 72, 98
Eco130I CCWWGG 1 cut(s) 179
Eco24I GRGCYC 2 cut(s) 74, 100
Eco53kI GAGCTC 2 cut(s) 72, 98
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 2 cut(s) 72, 98
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 179
EcoT22I ATGCAT 1 cut(s) 251
EcoT38I GRGCYC 2 cut(s) 74, 100
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 7 cut(s) 104, 116, 136, 164, 249, 366, 586
FatI CATG 7 cut(s) 100, 112, 132, 160, 245, 362, 582
FauI CCCGC 1 cut(s) 137
FbaI TGATCA 2 cut(s) 103, 115
Fnu4HI GCNGC 2 cut(s) 389, 479
FokI GGATG 4 cut(s) 152, 522, 527, 539
FriOI GRGCYC 2 cut(s) 74, 100
Fsp4HI GCNGC 2 cut(s) 389, 479
GlaI GCGC 1 cut(s) 454
GluI GCNGC 2 cut(s) 389, 479
GsuI CTGGAG 1 cut(s) 58
HaeIII GGCC 4 cut(s) 192, 261, 503, 554
HapII CCGG 4 cut(s) 50, 65, 137, 557
HhaI GCGC 1 cut(s) 455
Hin1II CATG 7 cut(s) 104, 116, 136, 164, 249, 366, 586
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HinfI GANTC 2 cut(s) 409, 586
HpaII CCGG 4 cut(s) 50, 65, 137, 557
HphI GGTGA 4 cut(s) 79, 98, 244, 431
Hpy166II GTNNAC 3 cut(s) 36, 321, 433
Hpy188I TCNGA 2 cut(s) 211, 509
Hpy188III TCNNGA 3 cut(s) 101, 119, 590
Hpy8I GTNNAC 3 cut(s) 36, 321, 433
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 2 cut(s) 318, 473
HpyCH4V TGCA 5 cut(s) 228, 249, 354, 481, 530
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 311, 372, 450, 459
HpyF3I CTNAG 2 cut(s) 270, 383
Hsp92II CATG 7 cut(s) 104, 116, 136, 164, 249, 366, 586
HspAI GCGC 1 cut(s) 453
Ksp22I TGATCA 2 cut(s) 103, 115
Kzo9I GATC 3 cut(s) 103, 115, 211
LmnI GCTCC 6 cut(s) 49, 77, 95, 148, 311, 380
Lsp1109I GCAGC 2 cut(s) 400, 465
LweI GCATC 5 cut(s) 31, 130, 258, 363, 517
MaeIII GTNAC 1 cut(s) 337
MalI GATC 3 cut(s) 105, 117, 213
MbiI CCGCTC 1 cut(s) 375
MboI GATC 3 cut(s) 103, 115, 211
MboII GAAGA 1 cut(s) 416
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 4 cut(s) 74, 100, 316, 564
MlsI TGGCCA 2 cut(s) 261, 503
MluCI AATT 1 cut(s) 19
MluNI TGGCCA 2 cut(s) 261, 503
MlyI GAGTC 2 cut(s) 418, 595
MnlI CCTC 6 cut(s) 16, 57, 156, 203, 378, 568
Mox20I TGGCCA 2 cut(s) 261, 503
Mph1103I ATGCAT 1 cut(s) 251
MscI TGGCCA 2 cut(s) 261, 503
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 3 cut(s) 111, 159, 238
Msp20I TGGCCA 2 cut(s) 261, 503
MspI CCGG 4 cut(s) 50, 65, 137, 557
MspR9I CCNGG 2 cut(s) 464, 557
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 5 cut(s) 50, 311, 372, 450, 459
NciI CCSGG 1 cut(s) 557
NdeII GATC 3 cut(s) 103, 115, 211
NlaIII CATG 7 cut(s) 104, 116, 136, 164, 249, 366, 586
NlaIV GGNNCC 4 cut(s) 213, 536, 555, 561
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 251
NspI RCATGY 1 cut(s) 136
PaeI GCATGC 1 cut(s) 136
PagI TCATGA 1 cut(s) 100
PflMI CCANNNNNTGG 2 cut(s) 510, 544
PkrI GCNGC 2 cut(s) 390, 480
PleI GAGTC 2 cut(s) 417, 594
PpsI GAGTC 2 cut(s) 417, 594
Psp124BI GAGCTC 2 cut(s) 74, 100
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 4 cut(s) 213, 536, 555, 561
PspPI GGNCC 1 cut(s) 553
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 3 cut(s) 111, 159, 238
SacI GAGCTC 2 cut(s) 74, 100
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 2 cut(s) 389, 479
Sau3AI GATC 3 cut(s) 103, 115, 211
Sau96I GGNCC 1 cut(s) 553
SchI GAGTC 2 cut(s) 418, 595
ScrFI CCNGG 2 cut(s) 464, 557
SduI GDGCHC 4 cut(s) 74, 100, 316, 564
SetI ASST 7 cut(s) 74, 100, 224, 257, 326, 334, 480
SfaNI GCATC 5 cut(s) 31, 130, 258, 363, 517
SmiMI CAYNNNNRTG 3 cut(s) 111, 159, 238
SphI GCATGC 1 cut(s) 136
Sse9I AATT 1 cut(s) 19
SsiI CCGC 3 cut(s) 130, 373, 489
SstI GAGCTC 2 cut(s) 74, 100
StyD4I CCNGG 2 cut(s) 462, 555
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 2 cut(s) 318, 473
TasI AATT 1 cut(s) 19
TatI WGTACW 1 cut(s) 318
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 321, 440
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 2 cut(s) 388, 478
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 321, 440
Van91I CCANNNNNTGG 2 cut(s) 510, 544
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.