AT2G04400

Indole-3-glycerol phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008243
Physical Location & Seq
Forward (+)
1531148 .. 1533737
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04400.1

Sequence Viewer

Length: 1209 bp
ATGGAGGGACTAGTTCCGGTACAGAGACTTCCAATTAAAGTGGCTTCACCTTCTCTGTATCGGTGTAACAATTCTGTCTCAATCAGACGTTCAATTTCAGGTTTTGCCATGGACAGGAAGATAAACTTTAGGGCTCCGTCGCAATTCTCGATCCGAGCTCAACAGTCGGATTTGAAGGAAAGCTTAGCTGTGTCTTCTTCATCAGTAGAGGATAAAGGAAATGTTCTTAGAATCAAGGAATGGGAAGTAGAGATGTATCAGGAGGAATTAGCTATTAGTCAAGGTATTAGGATAAGGAGAAAACCACCAAGTAAGGCTCCTTTGGGATACTCTGGACCATTTGAGTTGAGATTGCATAACAATGATGCTGATTCTCCTCGTAATATCTTGGAGGAGATCACATGGTACAAAGACGTAGAAGTTTCCCGGATGAAGGAGCTAAATCCGCTTGACGTGCTGAAGAAAGCTGTAGAGGATGCTCCTCCTACTAGGGATTTTGTTGGGGCTCTTAGGATGGCTCATAAAAGAACTGGCTTCCCTGGCTTGATAGCTGAGGTTAAGAAGGCTTCTCCAAGTAGAGGAATCTTAAAAGAGAATTTTGACCCGGTCGAGATTGCTCAAGCTTATGAAAAAGGCGGAGCAGCATGCCTCAGCGTTTTGACAGACCAGAAGTATTTCCAGGGAGGCTTTGAAAACTTGGAAGCAATAAGGAGCGCTGGTGTGAAGTGTCCACTATTATGCAAAGAGTTTGTTGTAGATCCATGGCAGATCTACTATGCTCGGACTAAAGGCGCAGATGCAGTACTGCTTATTGCTGCTGTATTGGCTGACCTAGAAATAACCTTCTTGCTTAAGATTTGCAAGAAGCTTAGCTTGGCTGCCCTTGTTGAGGTACATGATGAGAGAGAGATGGGTCGTGTACTTGGAATAGAAGGAATCGAGCTTGTTGGCATCAATAACCGAAGTCTTGAAACATTTGAAGTGGACATCAGTAATACAAAGAAGCTTCTTGAAGGAGAGCATGGTAGACAAATCCGTGAGAGAGACATGATTGTGGTTGGCGAATCTGGTCTGTTTACACCAGATGACATTGCCTATGTACAAGCAGCCGGAGTCAAAGCAGTTTTGGTTGGAGAATCCATTGTGAAGCAGAACGACCCTGAGAAAGGAATAGCTGGACTTTTTGGCAGGAACATTTCTCATACTTAG

Protein Analysis

402

Amino Acids

44.58

Weight (kDa)

6.99

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IGPS PF00218 130 - 392 2.8e-73 Indole-3-glycerol phosphate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1027
AciI CCGC 2 cut(s) 446, 636
AclWI GGATC 2 cut(s) 145, 752
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 479
AfaI GTAC 6 cut(s) 21, 407, 804, 894, 921, 1101
AfeI AGCGCT 1 cut(s) 715
AfiI CCNNNNNNNGG 5 cut(s) 114, 433, 578, 889, 1166
AflII CTTAAG 1 cut(s) 851
AgsI TTSAA 6 cut(s) 93, 175, 692, 971, 980, 1013
AhlI ACTAGT 1 cut(s) 10
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 2 cut(s) 538, 678
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 3 cut(s) 19, 82, 1038
AlwI GGATC 2 cut(s) 145, 752
Aor51HI AGCGCT 1 cut(s) 715
ApeKI GCWGC 4 cut(s) 641, 815, 878, 1106
ApoI RAATTY 1 cut(s) 595
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 2 cut(s) 716, 794
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 2 cut(s) 427, 605
AsuHPI GGTGA 1 cut(s) 39
AvaII GGWCC 1 cut(s) 335
BanII GRGCYC 3 cut(s) 136, 160, 508
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 160
BbvCI CCTCAGC 2 cut(s) 552, 650
BbvI GCAGC 4 cut(s) 653, 802, 865, 1118
BccI CCATC 2 cut(s) 508, 904
BciT130I CCWGG 2 cut(s) 540, 680
BciVI GTATCC 1 cut(s) 320
BcnI CCSGG 2 cut(s) 427, 605
BcoDI GTCTC 3 cut(s) 19, 82, 1038
BcuI ACTAGT 1 cut(s) 10
BfaI CTAG 3 cut(s) 11, 489, 833
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 851
BfuI GTATCC 1 cut(s) 320
BglII AGATCT 1 cut(s) 768
BisI GCNGC 4 cut(s) 642, 816, 879, 1107
BlpI GCTNAGC 2 cut(s) 184, 869
BlsI GCNGC 4 cut(s) 643, 817, 880, 1108
BmcAI AGTACT 1 cut(s) 804
Bme1390I CCNGG 4 cut(s) 427, 540, 605, 680
Bme18I GGWCC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 135, 318
BmrFI CCNGG 4 cut(s) 427, 540, 605, 680
BmsI GCATC 4 cut(s) 355, 466, 787, 960
BpiI GAAGAC 1 cut(s) 186
Bpu10I CCTNAGC 2 cut(s) 552, 650
Bpu1102I GCTNAGC 2 cut(s) 184, 869
BpuEI CTTGAG 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 427, 605
BsaJI CCNNGG 4 cut(s) 108, 538, 679, 761
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 433, 578, 889, 1166
Bse1I ACTGG 1 cut(s) 535
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 2 cut(s) 540, 680
BseDI CCNNGG 4 cut(s) 108, 538, 679, 761
BseGI GGATG 3 cut(s) 435, 481, 519
BseLI CCNNNNNNNGG 5 cut(s) 114, 433, 578, 889, 1166
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 3 cut(s) 543, 664, 1152
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 3 cut(s) 366, 407, 471
BseXI GCAGC 4 cut(s) 653, 802, 865, 1118
Bsh1285I CGRYCG 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 609
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 4 cut(s) 17, 427, 605, 1110
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 5 cut(s) 114, 433, 578, 889, 1166
BsmAI GTCTC 3 cut(s) 19, 82, 1038
BsmFI GGGAC 1 cut(s) 21
Bsp1286I GDGCHC 3 cut(s) 136, 160, 508
Bsp1407I TGTACA 1 cut(s) 1099
Bsp143I GATC 4 cut(s) 150, 396, 757, 768
Bsp1720I GCTNAGC 2 cut(s) 184, 869
Bsp19I CCATGG 2 cut(s) 108, 761
BspACI CCGC 2 cut(s) 446, 636
BspCNI CTCAG 3 cut(s) 544, 663, 1153
BspLI GGNNCC 2 cut(s) 135, 318
BspPI GGATC 2 cut(s) 145, 752
BspTI CTTAAG 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 1099
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 4 cut(s) 108, 538, 679, 761
BssMI GATC 4 cut(s) 150, 396, 757, 768
BssT1I CCWWGG 2 cut(s) 108, 761
Bst2UI CCWGG 2 cut(s) 540, 680
Bst4CI ACNGT 1 cut(s) 165
BstAFI CTTAAG 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 1099
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 8 cut(s) 184, 227, 509, 552, 650, 869, 1161, 1206
BstDSI CCRYGG 2 cut(s) 108, 761
BstENI CCTNNNNNAGG 2 cut(s) 887, 1164
BstF5I GGATG 3 cut(s) 435, 481, 519
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 2 cut(s) 716, 794
BstKTI GATC 4 cut(s) 153, 399, 760, 771
BstMAI GTCTC 3 cut(s) 19, 82, 1038
BstMBI GATC 4 cut(s) 150, 396, 757, 768
BstMCI CGRYCG 1 cut(s) 609
BstMWI GCNNNNNNNGC 4 cut(s) 445, 454, 540, 824
BstNI CCWGG 2 cut(s) 540, 680
BstNSI RCATGY 1 cut(s) 648
BstSCI CCNGG 4 cut(s) 425, 538, 603, 678
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 4 cut(s) 653, 802, 865, 1118
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 757, 768
BstYI RGATCY 2 cut(s) 757, 768
BsuI GTATCC 1 cut(s) 320
BtgI CCRYGG 2 cut(s) 108, 761
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 3 cut(s) 435, 481, 519
Cac8I GCNNGC 1 cut(s) 646
CfoI GCGC 2 cut(s) 716, 794
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 6 cut(s) 20, 406, 803, 893, 920, 1100
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 7 cut(s) 109, 402, 645, 762, 896, 1022, 1048
CviQI GTAC 6 cut(s) 20, 406, 803, 893, 920, 1100
DdeI CTNAG 8 cut(s) 184, 227, 509, 552, 650, 869, 1161, 1206
DpnI GATC 4 cut(s) 152, 398, 759, 770
DpnII GATC 4 cut(s) 150, 396, 757, 768
EciI GGCGGA 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 108, 761
Eco24I GRGCYC 3 cut(s) 136, 160, 508
Eco47I GGWCC 1 cut(s) 335
Eco47III AGCGCT 1 cut(s) 715
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 158
EcoNI CCTNNNNNAGG 2 cut(s) 887, 1164
EcoRII CCWGG 2 cut(s) 538, 678
EcoT14I CCWWGG 2 cut(s) 108, 761
EcoT38I GRGCYC 3 cut(s) 136, 160, 508
ErhI CCWWGG 2 cut(s) 108, 761
FaeI CATG 7 cut(s) 112, 405, 648, 765, 899, 1025, 1051
FalI AAGNNNNNCTT 6 cut(s) 110, 142, 167, 199, 857, 889
FaqI GGGAC 1 cut(s) 21
FatI CATG 7 cut(s) 108, 401, 644, 761, 895, 1021, 1047
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 4 cut(s) 642, 816, 879, 1107
FokI GGATG 3 cut(s) 442, 488, 526
FriOI GRGCYC 3 cut(s) 136, 160, 508
Fsp4HI GCNGC 4 cut(s) 642, 816, 879, 1107
FspBI CTAG 3 cut(s) 11, 489, 833
GlaI GCGC 2 cut(s) 715, 793
GluI GCNGC 4 cut(s) 642, 816, 879, 1107
HaeII RGCGCY 1 cut(s) 717
HapII CCGG 4 cut(s) 17, 427, 605, 1110
HhaI GCGC 2 cut(s) 716, 794
Hin1II CATG 7 cut(s) 112, 405, 648, 765, 899, 1025, 1051
Hin6I GCGC 2 cut(s) 714, 792
HinP1I GCGC 2 cut(s) 714, 792
HindIII AAGCTT 4 cut(s) 181, 621, 866, 1004
HinfI GANTC 7 cut(s) 231, 371, 582, 936, 1064, 1113, 1136
HpaII CCGG 4 cut(s) 17, 427, 605, 1110
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 5 cut(s) 731, 920, 985, 1028, 1077
Hpy188I TCNGA 4 cut(s) 86, 155, 169, 783
Hpy188III TCNNGA 6 cut(s) 148, 260, 333, 610, 968, 1010
Hpy8I GTNNAC 5 cut(s) 731, 920, 985, 1028, 1077
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 7 cut(s) 60, 169, 427, 556, 853, 926, 1007
HpyCH4III ACNGT 1 cut(s) 165
HpyCH4IV ACGT 3 cut(s) 88, 414, 453
HpyCH4V TGCA 4 cut(s) 355, 741, 800, 861
HpyF10VI GCNNNNNNNGC 4 cut(s) 445, 454, 540, 824
HpyF3I CTNAG 8 cut(s) 184, 227, 509, 552, 650, 869, 1161, 1206
HpySE526I ACGT 3 cut(s) 88, 414, 453
Hsp92II CATG 7 cut(s) 112, 405, 648, 765, 899, 1025, 1051
HspAI GCGC 2 cut(s) 714, 792
Kzo9I GATC 4 cut(s) 150, 396, 757, 768
LmnI GCTCC 6 cut(s) 139, 322, 436, 484, 638, 711
Lsp1109I GCAGC 4 cut(s) 653, 802, 865, 1118
LweI GCATC 4 cut(s) 355, 466, 787, 960
MaeI CTAG 3 cut(s) 11, 489, 833
MaeII ACGT 3 cut(s) 88, 414, 453
MaeIII GTNAC 1 cut(s) 65
MalI GATC 4 cut(s) 152, 398, 759, 770
MboI GATC 4 cut(s) 150, 396, 757, 768
MboII GAAGA 4 cut(s) 130, 186, 189, 472
MflI RGATCY 2 cut(s) 757, 768
MhlI GDGCHC 3 cut(s) 136, 160, 508
MluCI AATT 6 cut(s) 33, 70, 93, 143, 266, 595
MlyI GAGTC 1 cut(s) 1122
MmeI TCCRAC 2 cut(s) 147, 1111
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 4 cut(s) 36, 558, 587, 852
MslI CAYNNNNRTG 3 cut(s) 360, 736, 1052
MspCI CTTAAG 1 cut(s) 851
MspI CCGG 4 cut(s) 17, 427, 605, 1110
MspR9I CCNGG 4 cut(s) 427, 540, 605, 680
MvaI CCWGG 2 cut(s) 540, 680
MwoI GCNNNNNNNGC 4 cut(s) 445, 454, 540, 824
NciI CCSGG 2 cut(s) 427, 605
NcoI CCATGG 2 cut(s) 108, 761
NdeII GATC 4 cut(s) 150, 396, 757, 768
NlaIII CATG 7 cut(s) 112, 405, 648, 765, 899, 1025, 1051
NlaIV GGNNCC 2 cut(s) 135, 318
NspI RCATGY 1 cut(s) 648
PaeI GCATGC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 6 cut(s) 231, 371, 582, 936, 1064, 1136
PflFI GACNNNGTC 1 cut(s) 605
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 4 cut(s) 643, 817, 880, 1108
PleI GAGTC 1 cut(s) 1121
PpsI GAGTC 1 cut(s) 1121
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 538, 678
PspGI CCWGG 2 cut(s) 538, 678
PspN4I GGNNCC 2 cut(s) 135, 318
PspPI GGNCC 1 cut(s) 335
PsuI RGATCY 2 cut(s) 757, 768
PsyI GACNNNGTC 1 cut(s) 605
RsaI GTAC 6 cut(s) 21, 407, 804, 894, 921, 1101
RsaNI GTAC 6 cut(s) 20, 406, 803, 893, 920, 1100
RseI CAYNNNNRTG 3 cut(s) 360, 736, 1052
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 4 cut(s) 36, 558, 587, 852
SatI GCNGC 4 cut(s) 642, 816, 879, 1107
Sau3AI GATC 4 cut(s) 150, 396, 757, 768
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 1 cut(s) 804
SchI GAGTC 1 cut(s) 1122
ScrFI CCNGG 4 cut(s) 427, 540, 605, 680
SduI GDGCHC 3 cut(s) 136, 160, 508
SfaNI GCATC 4 cut(s) 355, 466, 787, 960
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 3 cut(s) 360, 736, 1052
SmlI CTYRAG 2 cut(s) 618, 851
SmoI CTYRAG 2 cut(s) 618, 851
SpeI ACTAGT 1 cut(s) 10
SphI GCATGC 1 cut(s) 648
Sse9I AATT 6 cut(s) 33, 70, 93, 143, 266, 595
SsiI CCGC 2 cut(s) 446, 636
SspMI CTAG 3 cut(s) 11, 489, 833
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 4 cut(s) 425, 538, 603, 678
StyI CCWWGG 2 cut(s) 108, 761
TaaI ACNGT 1 cut(s) 165
TaiI ACGT 3 cut(s) 91, 417, 456
TaqI TCGA 3 cut(s) 149, 609, 939
TasI AATT 6 cut(s) 33, 70, 93, 143, 266, 595
TatI WGTACW 3 cut(s) 802, 919, 1099
TfiI GAWTC 6 cut(s) 231, 371, 582, 936, 1064, 1136
Tru1I TTAA 4 cut(s) 36, 558, 587, 852
Tru9I TTAA 4 cut(s) 36, 558, 587, 852
TseI GCWGC 4 cut(s) 641, 815, 878, 1106
TspDTI ATGAA 3 cut(s) 189, 446, 642
TspGWI ACGGA 2 cut(s) 126, 1025
Tth111I GACNNNGTC 1 cut(s) 605
Vha464I CTTAAG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 335
XagI CCTNNNNNAGG 2 cut(s) 887, 1164
XapI RAATTY 1 cut(s) 595
XceI RCATGY 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 1027
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 3 cut(s) 11, 489, 833
ZrmI AGTACT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.