AT2G05060

Protein kinase, catalytic domain (InterPro IPR000719), Serine threonine-protein kinase-like domain (InterPro IPR017442), Protein kinase-like domain (InterPro IPR011009), Serine threonine-protein kinase, active site (InterPro IPR008271)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0018742
Physical Location & Seq
Forward (+)
1798155 .. 1799102
948 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G05060.1

Sequence Viewer

Length: 948 bp
ATGAGAATTGATCTTTCTGTTCCAAAGGTGGGGGCAGAGTTAGAACTCAACAAGGTTCTCGGAAAAGGTTCGTCTGGTTCGGTGAGTCTCATCAAGTACAAGAGTCGACTCGACGGCCAAACACTTTACGCCGCGGTGAAAACCTCCAACATAATTCACGCCGACTCTCTCCTCAAAGAGTTTCAGATCTTGTCCGAATTCAAAGGCTGTTCGAGAATCGTCCAATGCTATGGGACAAAAGTACAAGAGACAATCAACGAAGAAGGTGACGTAGAGTTCACGATTCCCATGGAATATGCATCTGGAGGAAGCTTGAGACATTTTATGAGTAGATTCAAGGACATGAAATTGCCTGATGCTTTGATCAGAAGGTTTACGCGTATGATCCTCGAGGGTTTAGCAGTTATTCATGGACACGGTTATGTTCACTGCGATCTAAAACCCGAAAACATCCTTGTTTTCCCGAGTTTCGAGTTAAAGATTTCTGATTTCGGGTTGTCTAAAAGAGAAGGAGATAGTAAATGGTGGCTTCCTAGTCATCCGTTTGCGGGAACACCGGTATATATGTCGCCTGAATCGATTTCTAACGGCGAAACAAGGAGAGGTTTGGATTTATGGTCATTAGGATGTGTGGTGTTGGAGATGTACACTGGAAAGAGACCATGGTGGGATAAGAACTATGACTTGGGAGATCTTAAGAAAGGTTCCATGCCATTGATTTCGAAAGATATTCCTTGTGATGCAAAACTCTTTGTGATGACTTGCTTCGCGTCGGAGACTAACAAGAGAAAAAACGCTTTCACATTGTTGAGGCATTGCTTCTTGCGTGGGGATGTTAATAAGATCATAGAGCCTCTCGTGAAGAGTAAGAATTCTAATGATATAGCACTAGAGTTGGAGAATATTGAACACAGGCTTTCGCAACTTAGGTGTATTAGTGGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

315

Amino Acids

35.5

Weight (kDa)

8.66

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 14 - 267 8.1e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 15 - 274 3.3e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018742)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05060 AT2G34290 AT2G41910 AT2G41920 AT2G41930 AT2G42550 AT5G12090 AT5G27510 AT5G27790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 389
AccI GTMKAC 1 cut(s) 106
AccII CGCG 3 cut(s) 134, 379, 770
AciI CCGC 3 cut(s) 132, 134, 548
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 197, 871
AfaI GTAC 3 cut(s) 98, 243, 647
AfiI CCNNNNNNNGG 2 cut(s) 29, 548
AflII CTTAAG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 377
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 3 cut(s) 202, 337, 908
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 1 cut(s) 312
AluI AGCT 1 cut(s) 312
Alw26I GTCTC 5 cut(s) 92, 242, 310, 652, 770
AlwI GGATC 1 cut(s) 379
Ama87I CYCGRG 2 cut(s) 389, 463
AoxI GGCC 1 cut(s) 115
ApoI RAATTY 2 cut(s) 197, 871
AsiGI ACCGGT 1 cut(s) 556
Asp700I GAANNNNTTC 1 cut(s) 67
AsuHPI GGTGA 3 cut(s) 94, 148, 278
AsuII TTCGAA 1 cut(s) 722
AvaI CYCGRG 2 cut(s) 389, 463
BauI CACGAG 1 cut(s) 857
BceAI ACGGC 2 cut(s) 130, 604
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 5 cut(s) 92, 242, 310, 652, 770
BfaI CTAG 2 cut(s) 534, 890
BfrI CTTAAG 1 cut(s) 695
BglII AGATCT 2 cut(s) 186, 691
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 389, 463
BmiI GGNNCC 1 cut(s) 706
BmsI GCATC 3 cut(s) 308, 346, 730
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 334
Bsa29I ATCGAT 1 cut(s) 578
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 3 cut(s) 132, 288, 662
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 548
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 814
BseCI ATCGAT 1 cut(s) 578
BseDI CCNNGG 3 cut(s) 132, 288, 662
BseGI GGATG 4 cut(s) 450, 538, 632, 838
BseLI CCNNNNNNNGG 2 cut(s) 29, 548
BseMI GCAATG 1 cut(s) 814
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 161
Bsh1236I CGCG 3 cut(s) 134, 379, 770
BshFI GGCC 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 556
BshVI ATCGAT 1 cut(s) 578
BsiHKCI CYCGRG 2 cut(s) 389, 463
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 2 cut(s) 29, 548
BsmAI GTCTC 5 cut(s) 92, 242, 310, 652, 770
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 652
BsoBI CYCGRG 2 cut(s) 389, 463
Bsp119I TTCGAA 1 cut(s) 722
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
Bsp19I CCATGG 2 cut(s) 288, 662
BspACI CCGC 3 cut(s) 132, 134, 548
BspANI GGCC 1 cut(s) 117
BspDI ATCGAT 1 cut(s) 578
BspFNI CGCG 3 cut(s) 134, 379, 770
BspLI GGNNCC 1 cut(s) 706
BspPI GGATC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 722
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 814
BsrFI RCCGGY 1 cut(s) 556
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 132, 288, 662
BssMI GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 2 cut(s) 288, 662
Bst2BI CACGAG 1 cut(s) 857
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 857
BstAFI CTTAAG 1 cut(s) 695
BstAUI TGTACA 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 722
BstDEI CTNAG 1 cut(s) 926
BstDSI CCRYGG 3 cut(s) 132, 288, 662
BstF5I GGATG 4 cut(s) 450, 538, 632, 838
BstFNI CGCG 3 cut(s) 134, 379, 770
BstKTI GATC 7 cut(s) 13, 189, 366, 387, 436, 694, 846
BstMAI GTCTC 5 cut(s) 92, 242, 310, 652, 770
BstMBI GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
BstUI CGCG 3 cut(s) 134, 379, 770
BstX2I RGATCY 2 cut(s) 186, 691
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 2 cut(s) 186, 691
Bsu15I ATCGAT 1 cut(s) 578
BsuRI GGCC 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 578
BtgI CCRYGG 3 cut(s) 132, 288, 662
BtsCI GGATG 4 cut(s) 450, 538, 632, 838
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 427, 648
Cfr10I RCCGGY 1 cut(s) 556
Cfr42I CCGCGG 1 cut(s) 135
ClaI ATCGAT 1 cut(s) 578
CseI GACGC 1 cut(s) 759
Csp6I GTAC 3 cut(s) 97, 242, 646
CspAI ACCGGT 1 cut(s) 556
CviAII CATG 5 cut(s) 289, 343, 410, 663, 709
CviJI RGCY 6 cut(s) 117, 207, 312, 529, 853, 916
CviKI_1 RGCY 6 cut(s) 117, 207, 312, 529, 853, 916
CviQI GTAC 3 cut(s) 97, 242, 646
DdeI CTNAG 1 cut(s) 926
DpnI GATC 7 cut(s) 12, 188, 365, 386, 435, 693, 845
DpnII GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 857
Eco130I CCWWGG 2 cut(s) 288, 662
Eco31I GGTCTC 1 cut(s) 652
Eco88I CYCGRG 2 cut(s) 389, 463
EcoRI GAATTC 2 cut(s) 197, 871
EcoT14I CCWWGG 2 cut(s) 288, 662
EcoT22I ATGCAT 1 cut(s) 301
ErhI CCWWGG 2 cut(s) 288, 662
FaeI CATG 5 cut(s) 292, 346, 413, 666, 712
FaqI GGGAC 1 cut(s) 247
FatI CATG 5 cut(s) 288, 342, 409, 662, 708
FauI CCCGC 1 cut(s) 541
FbaI TGATCA 1 cut(s) 363
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 4 cut(s) 437, 525, 639, 845
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 2 cut(s) 534, 890
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 557
HgaI GACGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 292, 346, 413, 666, 712
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 8 cut(s) 85, 103, 108, 164, 216, 283, 333, 575
HpaII CCGG 1 cut(s) 557
HphI GGTGA 3 cut(s) 94, 148, 278
Hpy166II GTNNAC 5 cut(s) 107, 279, 375, 427, 648
Hpy188I TCNGA 6 cut(s) 62, 186, 196, 368, 487, 775
Hpy188III TCNNGA 5 cut(s) 213, 280, 303, 463, 859
Hpy8I GTNNAC 5 cut(s) 107, 279, 375, 427, 648
Hpy99I CGWCG 2 cut(s) 116, 775
HpyAV CCTTC 3 cut(s) 257, 363, 503
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 2 cut(s) 299, 743
HpyF3I CTNAG 1 cut(s) 926
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 5 cut(s) 292, 346, 413, 666, 712
Ksp22I TGATCA 1 cut(s) 363
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
LpnPI CCDG 7 cut(s) 60, 288, 366, 570, 585, 636, 898
LweI GCATC 3 cut(s) 308, 346, 730
MaeI CTAG 2 cut(s) 534, 890
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 266
MalI GATC 7 cut(s) 12, 188, 365, 386, 435, 693, 845
MboI GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
MboII GAAGA 2 cut(s) 272, 874
MflI RGATCY 2 cut(s) 186, 691
MluCI AATT 5 cut(s) 6, 153, 197, 347, 871
MluI ACGCGT 1 cut(s) 377
MlyI GAGTC 4 cut(s) 94, 102, 112, 158
MmeI TCCRAC 4 cut(s) 171, 618, 753, 876
MnlI CCTC 8 cut(s) 154, 182, 299, 385, 398, 596, 804, 864
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 67
MseI TTAA 3 cut(s) 476, 696, 837
MslI CAYNNNNRTG 2 cut(s) 420, 625
MspA1I CMGCKG 1 cut(s) 134
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 1 cut(s) 557
MvnI CGCG 3 cut(s) 134, 379, 770
NcoI CCATGG 2 cut(s) 288, 662
NdeII GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
NlaIII CATG 5 cut(s) 292, 346, 413, 666, 712
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 301
NspV TTCGAA 1 cut(s) 722
PaeR7I CTCGAG 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 67
PfeI GAWTC 4 cut(s) 216, 283, 333, 575
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 4 cut(s) 93, 102, 111, 158
PpsI GAGTC 4 cut(s) 93, 102, 111, 158
PspN4I GGNNCC 1 cut(s) 706
PspXI VCTCGAGB 1 cut(s) 389
PsuI RGATCY 2 cut(s) 186, 691
RsaI GTAC 3 cut(s) 98, 243, 647
RsaNI GTAC 3 cut(s) 97, 242, 646
RseI CAYNNNNRTG 2 cut(s) 420, 625
SacII CCGCGG 1 cut(s) 135
SalI GTCGAC 1 cut(s) 105
SaqAI TTAA 3 cut(s) 476, 696, 837
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 7 cut(s) 10, 186, 363, 384, 433, 691, 843
SchI GAGTC 4 cut(s) 94, 102, 112, 158
SfaNI GCATC 3 cut(s) 308, 346, 730
Sfr274I CTCGAG 1 cut(s) 389
Sfr303I CCGCGG 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 722
SgrBI CCGCGG 1 cut(s) 135
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 2 cut(s) 420, 625
SmlI CTYRAG 3 cut(s) 313, 389, 695
SmoI CTYRAG 3 cut(s) 313, 389, 695
Sse9I AATT 5 cut(s) 6, 153, 197, 347, 871
SsiI CCGC 3 cut(s) 132, 134, 548
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 534, 890
StyI CCWWGG 2 cut(s) 288, 662
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 1 cut(s) 273
TaqI TCGA 7 cut(s) 106, 111, 212, 390, 471, 578, 722
TasI AATT 5 cut(s) 6, 153, 197, 347, 871
TatI WGTACW 3 cut(s) 96, 241, 645
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 216, 283, 333, 575
Tru1I TTAA 3 cut(s) 476, 696, 837
Tru9I TTAA 3 cut(s) 476, 696, 837
TscAI CASTG 2 cut(s) 434, 655
TseFI GTSAC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 2 cut(s) 359, 398
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 2 cut(s) 434, 655
Vha464I CTTAAG 1 cut(s) 695
XapI RAATTY 2 cut(s) 197, 871
XhoI CTCGAG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 67
XspI CTAG 2 cut(s) 534, 890
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.