AT2G42550

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0018742
Physical Location & Seq
Forward (+)
17713196 .. 17714230
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42550.1

Sequence Viewer

Length: 1035 bp
ATGCAACCAAAAACAGAGTTCGTGAAGTTCTTGAGAAAAGGTGCGTATGGATCAGTCGATCTCGTCAAGTACATCAAACGCGACGATAACGCTTTGCCTCTTTACGCCGCCGTGAAAACCGCCGAATGTGAAGACTACAACTCTCTCGAAAGAGAAATTCAGATTCTATCCAAACTTGAAGGATGTCGAAGGATCGTGCAATGCTATGGGAATTATACTTTAGAAGAGGACTTCGATGTCGGTGGATTCCGAGTCTACAAGATGGTTATGGAGTACGCAGCTGCAGGGAGTTTATTCAGTTTCATGGACAGTTACAAAGATAGAAAGTTACCGGAAACGATGATTAAAGACTTTACTCGTATGATTCTTCAAGGATTAGTCTCTGTTCATAGACTCGGTTATGTTCATTGCGATCTAAAACCCGATAATCTACTTGTCTTTCCATGTAGACAATCTTACGAATTGAAGATTTCAGATTTCGGGTCTTCGAGAAAAGTCGGAGAATATTCTGATTGCTGGGATGTTGATTTGCCGTTTGTTGGAACGCCTGTTTACATGTCGCCAGAATCAGTCCGTAGCGGCGTGGCTGAGAAAGCCCTAGATTTGTGGTCGTTAGGGTGCATAGTATTGGAGATGTATACCGGTGTGATTCCATGGTCGGAGGTTGAATTTGAGGATCTTGCGCCTGCTCTATCAAAAGGTAAAGCGCCGGAGATTCCAAAGAGTTTACCGTGTGATGCAAGGAAGTTTCTAGAGACGTGTTTTTCAAGGAACCCTAAGGAGAGAGGAAGTGCTTCGGATTTATTGTCTCATCAGTTCTTGCGTGGTGAAGTGGTCTCCGGCTTCTCGCTTCCGCCGTTGAAGTTGAAGATTAAACTAGCACCGGAGAAACCGACGAATGTTTCGAAGAAGCCCTTGAAGCTAAAGATTATCCCACCAAAGCCCCCACAGTTTAAGAAAGGTGGGATTAAACCCTTGAAGTTGAAGATTATGCCTTTGAAGCCTCCAGCTTCTAGTTTCGTTTCTGTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000166 GO:0000186 GO:0000226 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010225 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010638 GO:0010646 GO:0010647 GO:0010941 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0030554 GO:0030587 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032153 GO:0032155 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032270 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032872 GO:0032874 GO:0033043 GO:0033044 GO:0033554 GO:0033674 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045935 GO:0045937 GO:0046328 GO:0046330 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070302 GO:0070304 GO:0071554 GO:0071704 GO:0071840 GO:0071852 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0080135 GO:0090702 GO:0097159 GO:0097367 GO:0097435 GO:0099120 GO:0099568 GO:0140096 GO:1900744 GO:1900745 GO:1901265 GO:1901363 GO:1901564 GO:1902531 GO:1902533 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

344

Amino Acids

38.77

Weight (kDa)

8.93

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 7 - 270 4.1e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 10 - 274 9.2e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018742)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05060 AT2G34290 AT2G41910 AT2G41920 AT2G41930 AT2G42550 AT5G12090 AT5G27510 AT5G27790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccI GTMKAC 3 cut(s) 255, 448, 638
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 108, 120, 579, 854
AclWI GGATC 3 cut(s) 58, 200, 684
AcsI RAATTY 2 cut(s) 156, 668
AfaI GTAC 2 cut(s) 71, 275
AfiI CCNNNNNNNGG 1 cut(s) 539
AflIII ACRYGT 2 cut(s) 555, 758
AgeI ACCGGT 1 cut(s) 641
AjiI CACGTC 1 cut(s) 759
AluBI AGCT 3 cut(s) 281, 922, 1010
AluI AGCT 3 cut(s) 281, 922, 1010
Alw26I GTCTC 4 cut(s) 385, 749, 813, 841
AlwI GGATC 3 cut(s) 58, 200, 684
ApeKI GCWGC 2 cut(s) 278, 281
ApoI RAATTY 2 cut(s) 156, 668
ArsI GACNNNNNNTTYG 2 cut(s) 886, 918
AsiGI ACCGGT 1 cut(s) 641
Asp700I GAANNNNTTC 1 cut(s) 793
AspLEI GCGC 2 cut(s) 685, 709
AsuHPI GGTGA 1 cut(s) 839
AsuII TTCGAA 1 cut(s) 905
AxyI CCTNAGG 1 cut(s) 777
BbsI GAAGAC 2 cut(s) 138, 477
BbvI GCAGC 2 cut(s) 268, 290
BccI CCATC 1 cut(s) 256
BceAI ACGGC 3 cut(s) 95, 517, 841
BcoDI GTCTC 4 cut(s) 385, 749, 813, 841
BfaI CTAG 4 cut(s) 599, 752, 878, 1014
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 710
BisI GCNGC 4 cut(s) 108, 279, 282, 580
BlsI GCNGC 4 cut(s) 109, 280, 283, 581
BmgBI CACGTC 1 cut(s) 759
BmiI GGNNCC 1 cut(s) 773
BmsI GCATC 1 cut(s) 727
BpiI GAAGAC 2 cut(s) 138, 477
BpmI CTGGAG 1 cut(s) 990
Bpu14I TTCGAA 1 cut(s) 905
BpuEI CTTGAG 1 cut(s) 52
BsaI GGTCTC 1 cut(s) 841
BsaJI CCNNGG 1 cut(s) 653
BsaWI WCCGGW 3 cut(s) 331, 641, 883
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse118I RCCGGY 1 cut(s) 641
Bse21I CCTNAGG 1 cut(s) 777
Bse3DI GCAATG 2 cut(s) 206, 406
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 2 cut(s) 188, 526
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMI GCAATG 2 cut(s) 206, 406
BseMII CTCAG 1 cut(s) 579
BseXI GCAGC 2 cut(s) 268, 290
BseYI CCCAGC 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 81
BshTI ACCGGT 1 cut(s) 641
BsiSI CCGG 5 cut(s) 332, 642, 710, 840, 884
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 4 cut(s) 385, 749, 813, 841
BsmBI CGTCTC 1 cut(s) 749
Bso31I GGTCTC 1 cut(s) 841
Bsp119I TTCGAA 1 cut(s) 905
Bsp143I GATC 5 cut(s) 50, 58, 192, 412, 676
Bsp19I CCATGG 1 cut(s) 653
BspACI CCGC 4 cut(s) 108, 120, 579, 854
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 773
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 3 cut(s) 58, 200, 684
BspT104I TTCGAA 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 841
BsrDI GCAATG 2 cut(s) 206, 406
BsrFI RCCGGY 1 cut(s) 641
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 5 cut(s) 50, 58, 192, 412, 676
BssNAI GTATAC 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 653
Bst1107I GTATAC 1 cut(s) 639
Bst4CI ACNGT 3 cut(s) 311, 732, 951
Bst6I CTCTTC 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 905
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 2 cut(s) 588, 777
BstDSI CCRYGG 1 cut(s) 653
BstF5I GGATG 2 cut(s) 188, 526
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 2 cut(s) 685, 709
BstKTI GATC 5 cut(s) 53, 61, 195, 415, 679
BstMAI GTCTC 4 cut(s) 385, 749, 813, 841
BstMBI GATC 5 cut(s) 50, 58, 192, 412, 676
BstMWI GCNNNNNNNGC 3 cut(s) 593, 919, 1000
BstNSI RCATGY 1 cut(s) 559
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 268, 290
BstV2I GAAGAC 2 cut(s) 138, 477
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZ17I GTATAC 1 cut(s) 639
Bsu36I CCTNAGG 1 cut(s) 777
BtgI CCRYGG 1 cut(s) 653
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 2 cut(s) 188, 526
Cac8I GCNNGC 1 cut(s) 687
CfoI GCGC 2 cut(s) 685, 709
Cfr10I RCCGGY 1 cut(s) 641
Csp6I GTAC 2 cut(s) 70, 274
CspAI ACCGGT 1 cut(s) 641
CviAII CATG 4 cut(s) 304, 444, 556, 654
CviJI RGCY 9 cut(s) 281, 587, 596, 843, 913, 922, 943, 1003, 1010
CviKI_1 RGCY 9 cut(s) 281, 587, 596, 843, 913, 922, 943, 1003, 1010
CviQI GTAC 2 cut(s) 70, 274
DdeI CTNAG 2 cut(s) 588, 777
DpnI GATC 5 cut(s) 52, 60, 194, 414, 678
DpnII GATC 5 cut(s) 50, 58, 192, 412, 676
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 1 cut(s) 843
Eco130I CCWWGG 1 cut(s) 653
Eco31I GGTCTC 1 cut(s) 841
Eco81I CCTNAGG 1 cut(s) 777
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 4 cut(s) 307, 447, 559, 657
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FatI CATG 4 cut(s) 303, 443, 555, 653
FblI GTMKAC 3 cut(s) 255, 448, 638
Fnu4HI GCNGC 4 cut(s) 108, 279, 282, 580
FokI GGATG 2 cut(s) 195, 533
Fsp4HI GCNGC 4 cut(s) 108, 279, 282, 580
FspBI CTAG 4 cut(s) 599, 752, 878, 1014
GlaI GCGC 2 cut(s) 684, 708
GluI GCNGC 4 cut(s) 108, 279, 282, 580
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 1 cut(s) 710
HapII CCGG 5 cut(s) 332, 642, 710, 840, 884
HhaI GCGC 2 cut(s) 685, 709
Hin1II CATG 4 cut(s) 307, 447, 559, 657
Hin6I GCGC 2 cut(s) 683, 707
HinP1I GCGC 2 cut(s) 683, 707
HinfI GANTC 8 cut(s) 163, 246, 252, 364, 393, 566, 649, 715
HpaII CCGG 5 cut(s) 332, 642, 710, 840, 884
HphI GGTGA 1 cut(s) 839
Hpy166II GTNNAC 5 cut(s) 256, 449, 553, 639, 728
Hpy188I TCNGA 7 cut(s) 162, 251, 475, 500, 511, 661, 799
Hpy188III TCNNGA 5 cut(s) 22, 31, 146, 489, 752
Hpy8I GTNNAC 5 cut(s) 256, 449, 553, 639, 728
Hpy99I CGWCG 2 cut(s) 86, 898
HpyAV CCTTC 2 cut(s) 173, 183
HpyCH4III ACNGT 3 cut(s) 311, 732, 951
HpyCH4IV ACGT 1 cut(s) 758
HpyCH4V TGCA 5 cut(s) 4, 199, 284, 621, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 593, 919, 1000
HpyF3I CTNAG 2 cut(s) 588, 777
HpySE526I ACGT 1 cut(s) 758
Hsp92II CATG 4 cut(s) 307, 447, 559, 657
HspAI GCGC 2 cut(s) 683, 707
Kzo9I GATC 5 cut(s) 50, 58, 192, 412, 676
Lsp1109I GCAGC 2 cut(s) 268, 290
LweI GCATC 1 cut(s) 727
MaeI CTAG 4 cut(s) 599, 752, 878, 1014
MaeII ACGT 1 cut(s) 758
MaeIII GTNAC 2 cut(s) 311, 327
MalI GATC 5 cut(s) 52, 60, 194, 414, 678
MboI GATC 5 cut(s) 50, 58, 192, 412, 676
MboII GAAGA 8 cut(s) 143, 236, 359, 477, 478, 880, 919, 997
MflI RGATCY 1 cut(s) 676
MluCI AATT 4 cut(s) 156, 211, 461, 668
MlyI GAGTC 2 cut(s) 261, 387
MmeI TCCRAC 3 cut(s) 478, 520, 639
MnlI CCTC 6 cut(s) 108, 220, 655, 667, 779, 1014
MroXI GAANNNNTTC 1 cut(s) 793
MseI TTAA 4 cut(s) 345, 873, 954, 969
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 5 cut(s) 332, 642, 710, 840, 884
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 593, 919, 1000
NcoI CCATGG 1 cut(s) 653
NdeII GATC 5 cut(s) 50, 58, 192, 412, 676
NlaIII CATG 4 cut(s) 307, 447, 559, 657
NlaIV GGNNCC 1 cut(s) 773
NspI RCATGY 1 cut(s) 559
NspV TTCGAA 1 cut(s) 905
PciI ACATGT 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 854, 902
PdmI GAANNNNTTC 1 cut(s) 793
PfeI GAWTC 6 cut(s) 163, 246, 364, 566, 649, 715
PinAI ACCGGT 1 cut(s) 641
PkrI GCNGC 4 cut(s) 109, 280, 283, 581
PleI GAGTC 2 cut(s) 260, 387
PpsI GAGTC 2 cut(s) 260, 387
PscI ACATGT 1 cut(s) 555
PspFI CCCAGC 1 cut(s) 516
PspN4I GGNNCC 1 cut(s) 773
PstI CTGCAG 1 cut(s) 286
PsuI RGATCY 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 2 cut(s) 71, 275
RsaNI GTAC 2 cut(s) 70, 274
SaqAI TTAA 4 cut(s) 345, 873, 954, 969
SatI GCNGC 4 cut(s) 108, 279, 282, 580
Sau3AI GATC 5 cut(s) 50, 58, 192, 412, 676
SchI GAGTC 2 cut(s) 261, 387
SetI ASST 8 cut(s) 43, 283, 666, 703, 761, 924, 964, 1012
SfaNI GCATC 1 cut(s) 727
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 4 cut(s) 156, 211, 461, 668
SsiI CCGC 4 cut(s) 108, 120, 579, 854
SspI AATATT 1 cut(s) 506
SspMI CTAG 4 cut(s) 599, 752, 878, 1014
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 3 cut(s) 311, 732, 951
TaiI ACGT 1 cut(s) 761
TaqI TCGA 6 cut(s) 57, 147, 187, 234, 488, 905
TasI AATT 4 cut(s) 156, 211, 461, 668
TatI WGTACW 1 cut(s) 69
TauI GCSGC 2 cut(s) 110, 582
TfiI GAWTC 6 cut(s) 163, 246, 364, 566, 649, 715
Tru1I TTAA 4 cut(s) 345, 873, 954, 969
Tru9I TTAA 4 cut(s) 345, 873, 954, 969
TseI GCWGC 2 cut(s) 278, 281
TspDTI ATGAA 3 cut(s) 292, 377, 395
TspGWI ACGGA 1 cut(s) 563
XapI RAATTY 2 cut(s) 156, 668
XbaI TCTAGA 1 cut(s) 751
XceI RCATGY 1 cut(s) 559
XmiI GTMKAC 3 cut(s) 255, 448, 638
XmnI GAANNNNTTC 1 cut(s) 793
XspI CTAG 4 cut(s) 599, 752, 878, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.