AT5G12090

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0018742
Physical Location & Seq
Forward (+)
3909703 .. 3910877
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12090.1

Sequence Viewer

Length: 1110 bp
ATGATCGAAAACGATGACTCTCCTCAGCATCCTTCTTTATTCTTCACTGACTTCACACGTGCCAACTCCCACGTGCGGGTTCTGCATCACAAATTCTTGGGAAAAGGTGTTTATGGTTCTGTTGATCTCATCAGATACACTAAAACCGATGGTTCCTCGTTACAAGCCGCCGTAAAAACATCTTACGCTGAAGATCTCGAAGAATACGACGCGCTCAAAAGAGAGATACAGATTCTGTCTGAACTCAAGGGATATCCCAACATCGTTATTTGCTATGGAGACGATTTGGAAGAAGATTTCAACGAACACGGACACAAAGTTTACAAATTGCTTCTTGAATACGCAAACGAAGGGAGTTTAAGTTCATTCATGGAGAATTATCCCGACAGAAAGTTACCAGATCCGATGATCAGAGACTTCACGCGGATGATTCTCGAAGGATTGGTCTCAATGCATAGTCACGGTTATGTTCATTGCGATCTCAAATCCGATAACCTTCTTATCTTCTCTCGGAAAGATTCTGCTTCGTGCGAGTTAAAGATTTTTGATTTTGGAAACTGTAGACAAGTCGGAGAAGTTCCGGATCACTGGAAAAGTGATTACCCGTATGTGGGAACGCCTGAGTCTTTCTTCGACGGTGTTGCCAAGAAAACCCTAGATTTGTGGTCGTTAGGGTGCTTAGTGTTGAAGATTTATACAGGTGAGCAGCCATGGGAAAGAGTTACTAGTGTCGATTTCGTGAATTTTCTTAGTGATGGTGAAGCTCCAAATATTCCTGAATATGTGCCTTGTGATGCAAGAGAGTTTATTGAAACGTGTTTCGCAAGGGAACATGAGAAGAGAGGTACCGCGTCTGAGTTGTTATTGCATCCGTTTTTGTGTCAGAAGCAACCCTTGCTGGTGAAGTTGAAATTGAGAATCAAACAAGCTTCGAAGATAACAATGGGTATTAGAGAGAAGCCCCTGACATTGAAGATGTTTCTATCTAAGCCTCCACAACTTAAAAAACTTTTGAGTAAACTTTTGAGGTTGAAGAAGATGATTCATCCGAAACCATTGGAATTGGATTCTAGTTTCGTTTCTTACAGTAGTGATCTCTGTTTATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000166 GO:0000186 GO:0000226 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010225 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010638 GO:0010646 GO:0010647 GO:0010941 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0030554 GO:0030587 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032153 GO:0032155 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032270 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032872 GO:0032874 GO:0033043 GO:0033044 GO:0033554 GO:0033674 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045935 GO:0045937 GO:0046328 GO:0046330 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070302 GO:0070304 GO:0071554 GO:0071704 GO:0071840 GO:0071852 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0080135 GO:0090702 GO:0097159 GO:0097367 GO:0097435 GO:0099120 GO:0099568 GO:0140096 GO:1900744 GO:1900745 GO:1901265 GO:1901363 GO:1901564 GO:1902531 GO:1902533 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

369

Amino Acids

42.34

Weight (kDa)

6.13

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 30 - 293 3.1e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 30 - 287 1.8e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018742)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05060 AT2G34290 AT2G41910 AT2G41920 AT2G41930 AT2G42550 AT5G12090 AT5G27510 AT5G27790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1108
Acc65I GGTACC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 845
AccI GTMKAC 1 cut(s) 562
AccII CGCG 3 cut(s) 212, 424, 851
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 4 cut(s) 76, 168, 424, 849
AclWI GGATC 2 cut(s) 395, 591
AcsI RAATTY 2 cut(s) 92, 742
AcuI CTGAAG 1 cut(s) 210
AcvI CACGTG 2 cut(s) 59, 73
AfaI GTAC 1 cut(s) 847
AfiI CCNNNNNNNGG 4 cut(s) 75, 76, 610, 611
AflIII ACRYGT 2 cut(s) 56, 815
AgsI TTSAA 7 cut(s) 301, 338, 688, 812, 910, 973, 1033
AhlI ACTAGT 1 cut(s) 725
AloI GAACNNNNNNTCC 2 cut(s) 346, 378
AluBI AGCT 2 cut(s) 764, 929
AluI AGCT 2 cut(s) 764, 929
Alw26I GTCTC 3 cut(s) 273, 408, 451
AlwI GGATC 2 cut(s) 395, 591
AlwNI CAGNNNCTG 1 cut(s) 235
Aor13HI TCCGGA 1 cut(s) 580
ApeKI GCWGC 1 cut(s) 706
ApoI RAATTY 2 cut(s) 92, 742
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
Asp718I GGTACC 1 cut(s) 845
AspLEI GCGC 1 cut(s) 214
AsuHPI GGTGA 3 cut(s) 713, 770, 913
AsuII TTCGAA 1 cut(s) 932
BanI GGYRCC 1 cut(s) 845
BbrPI CACGTG 2 cut(s) 59, 73
BbvCI CCTCAGC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 718
BccI CCATC 2 cut(s) 143, 749
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 3 cut(s) 273, 408, 451
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 3 cut(s) 656, 726, 1071
BfmI CTRYAG 1 cut(s) 559
BglII AGATCT 1 cut(s) 193
BisI GCNGC 2 cut(s) 168, 707
BlsI GCNGC 2 cut(s) 169, 708
BmiI GGNNCC 2 cut(s) 154, 847
BmsI GCATC 4 cut(s) 37, 94, 784, 877
Bpu10I CCTNAGC 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 932
BpuEI CTTGAG 1 cut(s) 230
BsaAI YACGTR 2 cut(s) 59, 73
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 710
BsaWI WCCGGW 1 cut(s) 580
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 76, 610, 611
Bse1I ACTGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 472
BseAI TCCGGA 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 4 cut(s) 28, 432, 868, 1045
BseLI CCNNNNNNNGG 4 cut(s) 75, 76, 610, 611
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 3 cut(s) 38, 612, 846
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 718
Bsh1236I CGCG 3 cut(s) 212, 424, 851
BshNI GGYRCC 1 cut(s) 845
BsiSI CCGG 1 cut(s) 581
BslI CCNNNNNNNGG 4 cut(s) 75, 76, 610, 611
BsmAI GTCTC 3 cut(s) 273, 408, 451
BsmBI CGTCTC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 451
Bsp119I TTCGAA 1 cut(s) 932
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 4 cut(s) 76, 168, 424, 849
BspCNI CTCAG 3 cut(s) 37, 613, 847
BspEI TCCGGA 1 cut(s) 580
BspFNI CGCG 3 cut(s) 212, 424, 851
BspLI GGNNCC 2 cut(s) 154, 847
BspPI GGATC 2 cut(s) 395, 591
BspT104I TTCGAA 1 cut(s) 932
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
BssT1I CCWWGG 1 cut(s) 710
Bst4CI ACNGT 4 cut(s) 464, 560, 638, 1088
Bst6I CTCTTC 1 cut(s) 833
BstAPI GCANNNNNTGC 1 cut(s) 895
BstBAI YACGTR 2 cut(s) 59, 73
BstBI TTCGAA 1 cut(s) 932
BstDEI CTNAG 6 cut(s) 24, 621, 679, 749, 855, 987
BstDSI CCRYGG 1 cut(s) 710
BstF5I GGATG 4 cut(s) 28, 432, 868, 1045
BstFNI CGCG 3 cut(s) 212, 424, 851
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 8 cut(s) 6, 127, 196, 403, 411, 481, 586, 1096
BstMAI GTCTC 3 cut(s) 273, 408, 451
BstMBI GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
BstMWI GCNNNNNNNGC 2 cut(s) 82, 895
BstSFI CTRYAG 1 cut(s) 559
BstUI CGCG 3 cut(s) 212, 424, 851
BstV1I GCAGC 1 cut(s) 718
BstX2I RGATCY 2 cut(s) 193, 400
BstYI RGATCY 2 cut(s) 193, 400
BtgI CCRYGG 1 cut(s) 710
BtsCI GGATG 4 cut(s) 28, 432, 868, 1045
BtsIMutI CAGTG 2 cut(s) 45, 586
CaiI CAGNNNCTG 1 cut(s) 235
CfoI GCGC 1 cut(s) 214
CseI GACGC 2 cut(s) 218, 840
Csp6I GTAC 1 cut(s) 846
CviAII CATG 3 cut(s) 370, 711, 833
CviJI RGCY 6 cut(s) 167, 709, 764, 929, 961, 991
CviKI_1 RGCY 6 cut(s) 167, 709, 764, 929, 961, 991
CviQI GTAC 1 cut(s) 846
DdeI CTNAG 6 cut(s) 24, 621, 679, 749, 855, 987
DpnI GATC 8 cut(s) 5, 126, 195, 402, 410, 480, 585, 1095
DpnII GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
Eco130I CCWWGG 1 cut(s) 710
Eco31I GGTCTC 1 cut(s) 451
Eco32I GATATC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 210
Eco72I CACGTG 2 cut(s) 59, 73
EcoRV GATATC 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 273
FaeI CATG 3 cut(s) 373, 714, 836
FalI AAGNNNNNCTT 2 cut(s) 878, 910
FatI CATG 3 cut(s) 369, 710, 832
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 168, 707
FokI GGATG 4 cut(s) 15, 439, 855, 1032
Fsp4HI GCNGC 2 cut(s) 168, 707
FspBI CTAG 3 cut(s) 656, 726, 1071
GlaI GCGC 1 cut(s) 213
GluI GCNGC 2 cut(s) 168, 707
HapII CCGG 1 cut(s) 581
HgaI GACGC 2 cut(s) 218, 840
HhaI GCGC 1 cut(s) 214
Hin1II CATG 3 cut(s) 373, 714, 836
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 8 cut(s) 17, 232, 430, 518, 623, 918, 1042, 1067
HpaII CCGG 1 cut(s) 581
HphI GGTGA 3 cut(s) 713, 770, 913
Hpy166II GTNNAC 3 cut(s) 322, 563, 1019
Hpy188III TCNNGA 7 cut(s) 197, 335, 383, 434, 581, 739, 776
Hpy8I GTNNAC 3 cut(s) 322, 563, 1019
Hpy99I CGWCG 2 cut(s) 212, 638
HpyAV CCTTC 4 cut(s) 42, 344, 431, 506
HpyCH4III ACNGT 4 cut(s) 464, 560, 638, 1088
HpyCH4IV ACGT 3 cut(s) 58, 72, 815
HpyCH4V TGCA 4 cut(s) 85, 454, 797, 868
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 895
HpyF3I CTNAG 6 cut(s) 24, 621, 679, 749, 855, 987
HpySE526I ACGT 3 cut(s) 58, 72, 815
Hsp92II CATG 3 cut(s) 373, 714, 836
HspAI GCGC 1 cut(s) 212
Kpn2I TCCGGA 1 cut(s) 580
KpnI GGTACC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
LmnI GCTCC 1 cut(s) 769
LpnPI CCDG 8 cut(s) 411, 574, 594, 633, 684, 789, 884, 977
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 4 cut(s) 37, 94, 784, 877
MaeI CTAG 3 cut(s) 656, 726, 1071
MaeII ACGT 3 cut(s) 58, 72, 815
MaeIII GTNAC 4 cut(s) 159, 393, 458, 721
MalI GATC 8 cut(s) 5, 126, 195, 402, 410, 480, 585, 1095
MboI GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
MflI RGATCY 2 cut(s) 193, 400
MluCI AATT 6 cut(s) 92, 326, 376, 742, 911, 1061
MlyI GAGTC 2 cut(s) 11, 632
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 5 cut(s) 33, 166, 836, 1002, 1020
Mph1103I ATGCAT 1 cut(s) 456
MroI TCCGGA 1 cut(s) 580
MseI TTAA 3 cut(s) 359, 536, 1002
MslI CAYNNNNRTG 2 cut(s) 425, 465
MspI CCGG 1 cut(s) 581
MvnI CGCG 3 cut(s) 212, 424, 851
MwoI GCNNNNNNNGC 2 cut(s) 82, 895
NcoI CCATGG 1 cut(s) 710
NdeII GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
NlaIII CATG 3 cut(s) 373, 714, 836
NlaIV GGNNCC 2 cut(s) 154, 847
NmuCI GTSAC 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 456
NspV TTCGAA 1 cut(s) 932
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 6 cut(s) 232, 430, 518, 918, 1042, 1067
PkrI GCNGC 2 cut(s) 169, 708
PleI GAGTC 2 cut(s) 11, 631
PmaCI CACGTG 2 cut(s) 59, 73
PmlI CACGTG 2 cut(s) 59, 73
PpsI GAGTC 2 cut(s) 11, 631
Ppu21I YACGTR 2 cut(s) 59, 73
PsiI TTATAA 1 cut(s) 1108
PspCI CACGTG 2 cut(s) 59, 73
PspN4I GGNNCC 2 cut(s) 154, 847
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 2 cut(s) 193, 400
RsaI GTAC 1 cut(s) 847
RsaNI GTAC 1 cut(s) 846
RseI CAYNNNNRTG 2 cut(s) 425, 465
SaqAI TTAA 3 cut(s) 359, 536, 1002
SatI GCNGC 2 cut(s) 168, 707
Sau3AI GATC 8 cut(s) 3, 124, 193, 400, 408, 478, 583, 1093
SchI GAGTC 2 cut(s) 11, 632
SfaNI GCATC 4 cut(s) 37, 94, 784, 877
SfcI CTRYAG 1 cut(s) 559
SfuI TTCGAA 1 cut(s) 932
SmiMI CAYNNNNRTG 2 cut(s) 425, 465
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SpeI ACTAGT 1 cut(s) 725
Sse9I AATT 6 cut(s) 92, 326, 376, 742, 911, 1061
SsiI CCGC 4 cut(s) 76, 168, 424, 849
SspI AATATT 1 cut(s) 772
SspMI CTAG 3 cut(s) 656, 726, 1071
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 4 cut(s) 464, 560, 638, 1088
TaiI ACGT 3 cut(s) 61, 75, 818
TaqI TCGA 6 cut(s) 6, 198, 435, 633, 732, 932
TasI AATT 6 cut(s) 92, 326, 376, 742, 911, 1061
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 6 cut(s) 232, 430, 518, 918, 1042, 1067
Tru1I TTAA 3 cut(s) 359, 536, 1002
Tru9I TTAA 3 cut(s) 359, 536, 1002
TscAI CASTG 2 cut(s) 52, 593
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 1 cut(s) 706
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 4 cut(s) 354, 358, 461, 1034
TspGWI ACGGA 2 cut(s) 324, 861
TspRI CASTG 2 cut(s) 52, 593
XapI RAATTY 2 cut(s) 92, 742
XmiI GTMKAC 1 cut(s) 562
XspI CTAG 3 cut(s) 656, 726, 1071
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.