AT5G27510

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0018742
Physical Location & Seq
Forward (+)
9713173 .. 9714078
906 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27510.1

Sequence Viewer

Length: 906 bp
ATGAAACTAAACGAAGAATACGTCAAGTTTTTAGGTGAAGGTGCTTATAGTTTCGTTGATCTCTTCAAGTACACAAAAAGCGACGGCTCTTCTTTTCACGCCGCCGTAAAAAGCTCCGATGATGAAAACTCTCTCCTTAAAGAATTTCATATTCTTTCCGAACTCAAAGGATGTCCAAGAATCATACAATGCTTTGGTAACGACTTGGAAGAGGGTTTCGACGATAAAGGAAACAGAGTCTACAAGTTGCTTCTCGAGTACGCATCCGAAGGTAGTTTAAGCGATTTCATGAACAATTGCGTGGATAGAAAGTTACCAGACTTGATGATTAGAGACTTCACGCGTATGATTCTCCAAGGTTTGGTATCGATTCATAGCCACGGTTATGTTCATTGCGATCTCAAGCCAGAAAATGTCCTTGTCTTCCCTTGTGGAGATTCTTACGAAGTGAAGATTTCTGATTTTGGACTCTCATTACAAGTCGGAGAAGTTCCTGATCACTGGAAAATCGAATACCCGTTTGTTGGAACGCTTAACTACATGCCTCCGGAGTCTCTACATGACGGTGTTGCCAATAAAACCCTAGATTTGTGGTCATTAGGGTGTTTAGTGTTGGAGATGTATGTGTGTAAGAAGCCATGGATAGGGTTTATACCTGAGGATTTTGTGTATATCCTCTCTAATGGTAACCCTCCAGAGATTCCCGAGAGTTTGCCTTGCGACGCAAGGGCGTTTATTCAAAAGTGTTTCTCAAGGAACCCTAAGGAGAGAGGTACTGCGTCTGAGCTGCTGTCTCATCGGTTTTTGCGTCAAGAAAAATCGAAATTGAAGATGATTTCTCCATTCAACCTGCTAAAGTTTAAGAAATTTTTAAACAAGCTCTTGAGGTTGAAGAATTTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000166 GO:0000186 GO:0000226 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010225 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010638 GO:0010646 GO:0010647 GO:0010941 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0030554 GO:0030587 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032153 GO:0032155 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032270 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032872 GO:0032874 GO:0033043 GO:0033044 GO:0033554 GO:0033674 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045935 GO:0045937 GO:0046328 GO:0046330 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070302 GO:0070304 GO:0071554 GO:0071704 GO:0071840 GO:0071852 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0080135 GO:0090702 GO:0097159 GO:0097367 GO:0097435 GO:0099120 GO:0099568 GO:0140096 GO:1900744 GO:1900745 GO:1901265 GO:1901363 GO:1901564 GO:1902531 GO:1902533 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

301

Amino Acids

34.52

Weight (kDa)

6.1

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 8 - 269 4.2e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 265 3.6e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018742)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05060 AT2G34290 AT2G41910 AT2G41920 AT2G41930 AT2G42550 AT5G12090 AT5G27510 AT5G27790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 858
AccB7I CCANNNNNTGG 1 cut(s) 361
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 343
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 1 cut(s) 102
AcsI RAATTY 3 cut(s) 143, 866, 895
AfaI GTAC 3 cut(s) 71, 260, 775
AfiI CCNNNNNNNGG 3 cut(s) 361, 524, 644
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 5 cut(s) 67, 740, 829, 847, 892
AluBI AGCT 3 cut(s) 114, 787, 880
AluI AGCT 3 cut(s) 114, 787, 880
Alw26I GTCTC 3 cut(s) 327, 558, 798
Ama87I CYCGRG 2 cut(s) 254, 704
Aor13HI TCCGGA 1 cut(s) 547
ApeKI GCWGC 1 cut(s) 787
ApoI RAATTY 3 cut(s) 143, 866, 895
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 896
AsuHPI GGTGA 1 cut(s) 47
AvaI CYCGRG 2 cut(s) 254, 704
AxyI CCTNAGG 2 cut(s) 657, 762
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 774
BceAI ACGGC 2 cut(s) 89, 100
BclI TGATCA 1 cut(s) 496
BcoDI GTCTC 3 cut(s) 327, 558, 798
BfaI CTAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 858
BisI GCNGC 2 cut(s) 102, 788
BlsI GCNGC 2 cut(s) 103, 789
BmeT110I CYCGRG 2 cut(s) 254, 704
BmiI GGNNCC 1 cut(s) 758
BmsI GCATC 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 415
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 3 cut(s) 386, 736, 904
Bsa29I ATCGAT 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 355, 379, 638
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 524, 644
Bse1I ACTGG 1 cut(s) 506
Bse21I CCTNAGG 2 cut(s) 657, 762
Bse3DI GCAATG 1 cut(s) 391
BseAI TCCGGA 1 cut(s) 547
BseCI ATCGAT 1 cut(s) 368
BseDI CCNNGG 3 cut(s) 355, 379, 638
BseGI GGATG 2 cut(s) 176, 263
BseLI CCNNNNNNNGG 3 cut(s) 361, 524, 644
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 648, 774
BseNI ACTGG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 774
Bsh1236I CGCG 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 254, 704
BsiSI CCGG 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 361, 524, 644
BsmAI GTCTC 3 cut(s) 327, 558, 798
BsoBI CYCGRG 2 cut(s) 254, 704
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 3 cut(s) 58, 397, 496
Bsp19I CCATGG 1 cut(s) 638
BspACI CCGC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 649, 775
BspDI ATCGAT 1 cut(s) 368
BspEI TCCGGA 1 cut(s) 547
BspFNI CGCG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 758
BspMI ACCTGC 1 cut(s) 858
BspQI GCTCTTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 391
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 355, 379, 638
BssMI GATC 3 cut(s) 58, 397, 496
BssT1I CCWWGG 2 cut(s) 355, 638
Bst4CI ACNGT 2 cut(s) 383, 566
Bst6I CTCTTC 3 cut(s) 68, 94, 204
BstDEI CTNAG 3 cut(s) 657, 762, 783
BstDSI CCRYGG 2 cut(s) 379, 638
BstEII GGTNACC 1 cut(s) 686
BstF5I GGATG 2 cut(s) 176, 263
BstFNI CGCG 1 cut(s) 343
BstKTI GATC 3 cut(s) 61, 400, 499
BstMAI GTCTC 3 cut(s) 327, 558, 798
BstMBI GATC 3 cut(s) 58, 397, 496
BstNSI RCATGY 1 cut(s) 544
BstPI GGTNACC 1 cut(s) 686
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 774
BstV2I GAAGAC 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 368
Bsu36I CCTNAGG 2 cut(s) 657, 762
BsuTUI ATCGAT 1 cut(s) 368
BtgI CCRYGG 2 cut(s) 379, 638
BtsCI GGATG 2 cut(s) 176, 263
BtsIMutI CAGTG 1 cut(s) 499
BveI ACCTGC 1 cut(s) 858
CciI TCATGA 1 cut(s) 288
ClaI ATCGAT 1 cut(s) 368
CseI GACGC 3 cut(s) 731, 768, 797
Csp6I GTAC 3 cut(s) 70, 259, 774
CviAII CATG 4 cut(s) 289, 541, 560, 639
CviJI RGCY 7 cut(s) 87, 114, 378, 406, 637, 787, 880
CviKI_1 RGCY 7 cut(s) 87, 114, 378, 406, 637, 787, 880
CviQI GTAC 3 cut(s) 70, 259, 774
DdeI CTNAG 3 cut(s) 657, 762, 783
DpnI GATC 3 cut(s) 60, 399, 498
DpnII GATC 3 cut(s) 58, 397, 496
DraI TTTAAA 1 cut(s) 873
Eam1104I CTCTTC 3 cut(s) 68, 94, 204
EarI CTCTTC 3 cut(s) 68, 94, 204
Eco130I CCWWGG 2 cut(s) 355, 638
Eco81I CCTNAGG 2 cut(s) 657, 762
Eco88I CYCGRG 2 cut(s) 254, 704
Eco91I GGTNACC 1 cut(s) 686
EcoO65I GGTNACC 1 cut(s) 686
EcoT14I CCWWGG 2 cut(s) 355, 638
ErhI CCWWGG 2 cut(s) 355, 638
FaeI CATG 4 cut(s) 292, 544, 563, 642
FatI CATG 4 cut(s) 288, 540, 559, 638
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 102, 788
FokI GGATG 2 cut(s) 183, 250
Fsp4HI GCNGC 2 cut(s) 102, 788
FspBI CTAG 1 cut(s) 584
GluI GCNGC 2 cut(s) 102, 788
GsuI CTGGAG 1 cut(s) 678
HapII CCGG 1 cut(s) 548
HgaI GACGC 3 cut(s) 731, 768, 797
Hin1II CATG 4 cut(s) 292, 544, 563, 642
HinfI GANTC 8 cut(s) 180, 237, 349, 370, 437, 468, 551, 700
HpaII CCGG 1 cut(s) 548
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 2 cut(s) 72, 241
Hpy188I TCNGA 7 cut(s) 118, 160, 268, 460, 485, 784, 905
Hpy188III TCNNGA 8 cut(s) 254, 289, 494, 548, 695, 704, 812, 883
Hpy8I GTNNAC 2 cut(s) 72, 241
Hpy99I CGWCG 3 cut(s) 86, 224, 725
HpyAV CCTTC 2 cut(s) 32, 263
HpyCH4III ACNGT 2 cut(s) 383, 566
HpyCH4IV ACGT 1 cut(s) 21
HpyF3I CTNAG 3 cut(s) 657, 762, 783
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 4 cut(s) 292, 544, 563, 642
Kpn2I TCCGGA 1 cut(s) 547
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 3 cut(s) 58, 397, 496
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 119
LpnPI CCDG 8 cut(s) 330, 420, 487, 507, 561, 669, 708, 863
Lsp1109I GCAGC 1 cut(s) 774
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 584
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 197, 312, 686
MalI GATC 3 cut(s) 60, 399, 498
MboI GATC 3 cut(s) 58, 397, 496
MboII GAAGA 8 cut(s) 26, 55, 81, 221, 415, 463, 841, 904
MfeI CAATTG 1 cut(s) 295
MluCI AATT 5 cut(s) 143, 295, 824, 866, 895
MluI ACGCGT 1 cut(s) 341
MlyI GAGTC 3 cut(s) 246, 462, 560
MmeI TCCRAC 3 cut(s) 463, 505, 594
MnlI CCTC 7 cut(s) 205, 555, 652, 686, 702, 764, 879
MroI TCCGGA 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 5 cut(s) 138, 278, 534, 861, 872
MslI CAYNNNNRTG 4 cut(s) 344, 384, 564, 601
MspI CCGG 1 cut(s) 548
MunI CAATTG 1 cut(s) 295
MvnI CGCG 1 cut(s) 343
NcoI CCATGG 1 cut(s) 638
NdeII GATC 3 cut(s) 58, 397, 496
NlaIII CATG 4 cut(s) 292, 544, 563, 642
NlaIV GGNNCC 1 cut(s) 758
NspI RCATGY 1 cut(s) 544
PaeR7I CTCGAG 1 cut(s) 254
PagI TCATGA 1 cut(s) 288
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 805
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 5 cut(s) 180, 349, 370, 437, 700
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 2 cut(s) 103, 789
PleI GAGTC 3 cut(s) 245, 462, 559
PpsI GAGTC 3 cut(s) 245, 462, 559
PspEI GGTNACC 1 cut(s) 686
PspN4I GGNNCC 1 cut(s) 758
RsaI GTAC 3 cut(s) 71, 260, 775
RsaNI GTAC 3 cut(s) 70, 259, 774
RseI CAYNNNNRTG 4 cut(s) 344, 384, 564, 601
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 5 cut(s) 138, 278, 534, 861, 872
SatI GCNGC 2 cut(s) 102, 788
Sau3AI GATC 3 cut(s) 58, 397, 496
SchI GAGTC 3 cut(s) 246, 462, 560
SfaNI GCATC 1 cut(s) 272
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 4 cut(s) 344, 384, 564, 601
SmlI CTYRAG 4 cut(s) 254, 401, 751, 883
SmoI CTYRAG 4 cut(s) 254, 401, 751, 883
Sse9I AATT 5 cut(s) 143, 295, 824, 866, 895
SsiI CCGC 1 cut(s) 102
SspMI CTAG 1 cut(s) 584
StyI CCWWGG 2 cut(s) 355, 638
TaaI ACNGT 2 cut(s) 383, 566
TaiI ACGT 1 cut(s) 24
TaqI TCGA 5 cut(s) 219, 255, 368, 510, 821
TasI AATT 5 cut(s) 143, 295, 824, 866, 895
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 5 cut(s) 180, 349, 370, 437, 700
Tru1I TTAA 5 cut(s) 138, 278, 534, 861, 872
Tru9I TTAA 5 cut(s) 138, 278, 534, 861, 872
TscAI CASTG 1 cut(s) 506
TseI GCWGC 1 cut(s) 787
TspDTI ATGAA 7 cut(s) 17, 137, 138, 277, 305, 362, 380
TspRI CASTG 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 361
XapI RAATTY 3 cut(s) 143, 866, 895
XceI RCATGY 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.