AT5G27790

Protein kinase, catalytic domain (InterPro IPR000719), Serine threonine-protein kinase-like domain (InterPro IPR017442), Protein kinase-like domain (InterPro IPR011009), Serine threonine-protein kinase, active site (InterPro IPR008271)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0018742
Physical Location & Seq
Reverse (-)
9840925 .. 9842003
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27790.1

Sequence Viewer

Length: 984 bp
ATGAATGAAGTCGCTATCAAAAGAATCAATCGTTCTATTTGCAATGGTGGCGTTGGATTTGGAGTTCGAAAGGTATTTGGGAAAGGTTCGTTTGGATCGGTGAGACTTTTCAGTTACAAAAGACGATGCGACGGTGAGACTCTCTACGCCACTGTGAAAACCTCTGATGATGCTAAGTCTCTCTACGAAGAGTTTCAGATCCTGTCTAAATTCAAGGGCTGTCCGAGAATCGTCCAATGCTACGGTAACGGAGTAAAACAGAGATTTAACGACAAGGGTTACGTAGAGTACATGATTCCTATGGAGTATGCAACCGGAGGAAGCTTGAACAATTTCATGGACCGATTCAACGACAGAAAATTGCCTGATCCGATGATCAGAAAGTTTACTCGTATGCTTCTCGAGGGTTTGGCCACAATTCACAGATACGGCTATGTTCACTACGATATCAAACCCGAGAACATACTTGTTTTCCCTGGTTCTGTCTACAAGGAGGGCGCGTGGAGATATTCTTACAAATTGAAGATTTCTGATTTCGGGTTGTCGAAAAGAGATGGAGACACTAAGTGGTGGCATCCTCTTAAATCGTATGCGGGAACACGGATCTATATGTCGCCGGAGTCTATCTCTCACGGGGAGATAGGGAAAGGACTTGACTTATGGTCGTTGGGATGTGTTGTGTTGGAGATGTACACTGGGAAGAGACCATGGTGGCATACTAACTATGAACTAGAAGATCTCATGAAGTGTTACGAGCCATTGTTTCCGCCTAATCTTCCTTGCGATGCAAAACTCTTTCTCATGACCTGCTTTGCGCCTGAACCAGATGAGAGGAAAGACGCGTTGACATTGTTGAGACAAAGCTTCTTTCGTCGAGATGTCAATAAGTTGACAAAGCTTCTGAAGGTTGATAATCGTAATGATTTCACCCTGGAACTGAAGAAACTTAGACAGATGCTTTCGGAAATTAGGAGTATGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

327

Amino Acids

38.13

Weight (kDa)

9.39

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 23 - 290 4.2e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 23 - 284 2.1e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018742)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05060 AT2G34290 AT2G41910 AT2G41920 AT2G41930 AT2G42550 AT5G12090 AT5G27510 AT5G27790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 815
AccI GTMKAC 1 cut(s) 486
AccII CGCG 2 cut(s) 500, 842
AciI CCGC 2 cut(s) 593, 767
AclWI GGATC 4 cut(s) 103, 193, 362, 611
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 1 cut(s) 209
AcuI CTGAAG 2 cut(s) 923, 959
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 2 cut(s) 290, 692
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 4 cut(s) 214, 328, 349, 523
AhdI GACNNNNNGTC 1 cut(s) 661
AjnI CCWGG 2 cut(s) 475, 930
AloI GAACNNNNNNTCC 2 cut(s) 48, 80
AluBI AGCT 3 cut(s) 324, 864, 898
AluI AGCT 3 cut(s) 324, 864, 898
Alw26I GTCTC 6 cut(s) 97, 131, 183, 552, 697, 850
AlwI GGATC 4 cut(s) 103, 193, 362, 611
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 2 cut(s) 401, 455
AoxI GGCC 1 cut(s) 411
ApoI RAATTY 1 cut(s) 209
Asp700I GAANNNNTTC 2 cut(s) 192, 332
AspLEI GCGC 2 cut(s) 500, 817
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 3 cut(s) 112, 146, 919
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 2 cut(s) 401, 455
AvaII GGWCC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 413
BccI CCATC 1 cut(s) 548
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 2 cut(s) 477, 932
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 6 cut(s) 97, 131, 183, 552, 697, 850
BfaI CTAG 1 cut(s) 731
BfuAI ACCTGC 1 cut(s) 815
BglII AGATCT 1 cut(s) 736
Bme1390I CCNGG 2 cut(s) 477, 932
Bme18I GGWCC 1 cut(s) 340
BmeRI GACNNNNNGTC 1 cut(s) 661
BmeT110I CYCGRG 2 cut(s) 401, 455
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 477, 932
BmrI ACTGGG 1 cut(s) 705
BmsI GCATC 5 cut(s) 116, 160, 583, 775, 945
BmuI ACTGGG 1 cut(s) 705
BplI GAGNNNNNCTC 2 cut(s) 611, 643
Bpu14I TTCGAA 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 3 cut(s) 475, 707, 930
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 3 cut(s) 485, 515, 964
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 2 cut(s) 477, 932
BseDI CCNNGG 3 cut(s) 475, 707, 930
BseGI GGATG 2 cut(s) 574, 677
BseMI GCAATG 1 cut(s) 49
BseNI ACTGG 1 cut(s) 700
Bsh1236I CGCG 2 cut(s) 500, 842
BshFI GGCC 1 cut(s) 413
BsiHKCI CYCGRG 2 cut(s) 401, 455
BsiSI CCGG 2 cut(s) 315, 617
BsmAI GTCTC 6 cut(s) 97, 131, 183, 552, 697, 850
BsnI GGCC 1 cut(s) 413
Bso31I GGTCTC 1 cut(s) 697
BsoBI CYCGRG 2 cut(s) 401, 455
Bsp119I TTCGAA 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 6 cut(s) 95, 198, 367, 375, 603, 736
Bsp19I CCATGG 1 cut(s) 707
BspACI CCGC 2 cut(s) 593, 767
BspANI GGCC 1 cut(s) 413
BspFNI CGCG 2 cut(s) 500, 842
BspHI TCATGA 2 cut(s) 741, 801
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 4 cut(s) 103, 193, 362, 611
BspT104I TTCGAA 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 697
BsrDI GCAATG 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 690
BsrI ACTGG 1 cut(s) 700
BssECI CCNNGG 3 cut(s) 475, 707, 930
BssMI GATC 6 cut(s) 95, 198, 367, 375, 603, 736
BssT1I CCWWGG 1 cut(s) 707
Bst2UI CCWGG 2 cut(s) 477, 932
Bst4CI ACNGT 3 cut(s) 134, 154, 245
Bst6I CTCTTC 2 cut(s) 183, 695
BstAUI TGTACA 1 cut(s) 690
BstBAI YACGTR 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 67
BstDEI CTNAG 3 cut(s) 174, 564, 947
BstDSI CCRYGG 1 cut(s) 707
BstF5I GGATG 2 cut(s) 574, 677
BstFNI CGCG 2 cut(s) 500, 842
BstHHI GCGC 2 cut(s) 500, 817
BstKTI GATC 6 cut(s) 98, 201, 370, 378, 606, 739
BstMAI GTCTC 6 cut(s) 97, 131, 183, 552, 697, 850
BstMBI GATC 6 cut(s) 95, 198, 367, 375, 603, 736
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 2 cut(s) 477, 932
BstSCI CCNGG 2 cut(s) 475, 930
BstSNI TACGTA 1 cut(s) 283
BstUI CGCG 2 cut(s) 500, 842
BstX2I RGATCY 3 cut(s) 198, 603, 736
BstYI RGATCY 3 cut(s) 198, 603, 736
BsuRI GGCC 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 707
BtgZI GCGATG 1 cut(s) 798
BtsCI GGATG 2 cut(s) 574, 677
BtsIMutI CAGTG 2 cut(s) 150, 693
BveI ACCTGC 1 cut(s) 815
CaiI CAGNNNCTG 1 cut(s) 202
CciI TCATGA 2 cut(s) 741, 801
CfoI GCGC 2 cut(s) 500, 817
Cfr13I GGNCC 1 cut(s) 340
CseI GACGC 1 cut(s) 848
Csp6I GTAC 2 cut(s) 289, 691
CviAII CATG 5 cut(s) 292, 337, 708, 742, 802
CviJI RGCY 7 cut(s) 219, 324, 413, 432, 757, 864, 898
CviKI_1 RGCY 7 cut(s) 219, 324, 413, 432, 757, 864, 898
CviQI GTAC 2 cut(s) 289, 691
DdeI CTNAG 3 cut(s) 174, 564, 947
DpnI GATC 6 cut(s) 97, 200, 369, 377, 605, 738
DpnII GATC 6 cut(s) 95, 198, 367, 375, 603, 736
DraIII CACNNNGTG 1 cut(s) 567
DriI GACNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 183, 695
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 2 cut(s) 183, 695
EciI GGCGGA 1 cut(s) 756
Eco105I TACGTA 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 707
Eco31I GGTCTC 1 cut(s) 697
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 923, 959
Eco88I CYCGRG 2 cut(s) 401, 455
EcoRII CCWGG 2 cut(s) 475, 930
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 707
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 5 cut(s) 295, 340, 711, 745, 805
FatI CATG 5 cut(s) 291, 336, 707, 741, 801
FauI CCCGC 1 cut(s) 586
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 1 cut(s) 486
FokI GGATG 2 cut(s) 561, 684
FspBI CTAG 1 cut(s) 731
GlaI GCGC 2 cut(s) 499, 816
HaeIII GGCC 1 cut(s) 413
HapII CCGG 2 cut(s) 315, 617
HgaI GACGC 1 cut(s) 848
HhaI GCGC 2 cut(s) 500, 817
Hin1II CATG 5 cut(s) 295, 340, 711, 745, 805
Hin6I GCGC 2 cut(s) 498, 815
HinP1I GCGC 2 cut(s) 498, 815
HincII GTYRAC 2 cut(s) 846, 891
HindII GTYRAC 2 cut(s) 846, 891
HindIII AAGCTT 3 cut(s) 322, 862, 896
HinfI GANTC 6 cut(s) 24, 139, 228, 295, 345, 620
HpaII CCGG 2 cut(s) 315, 617
HphI GGTGA 3 cut(s) 112, 146, 919
Hpy166II GTNNAC 6 cut(s) 387, 439, 487, 693, 846, 891
Hpy188I TCNGA 8 cut(s) 166, 198, 225, 372, 380, 532, 903, 964
Hpy188III TCNNGA 4 cut(s) 401, 742, 802, 875
Hpy8I GTNNAC 6 cut(s) 387, 439, 487, 693, 846, 891
Hpy99I CGWCG 2 cut(s) 134, 876
HpyAV CCTTC 1 cut(s) 898
HpyCH4III ACNGT 3 cut(s) 134, 154, 245
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 3 cut(s) 42, 311, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 3 cut(s) 174, 564, 947
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 5 cut(s) 295, 340, 711, 745, 805
HspAI GCGC 2 cut(s) 498, 815
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 6 cut(s) 95, 198, 367, 375, 603, 736
LweI GCATC 5 cut(s) 116, 160, 583, 775, 945
MaeI CTAG 1 cut(s) 731
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 4 cut(s) 113, 245, 278, 749
MalI GATC 6 cut(s) 97, 200, 369, 377, 605, 738
MboI GATC 6 cut(s) 95, 198, 367, 375, 603, 736
MboII GAAGA 6 cut(s) 200, 535, 712, 746, 767, 952
MflI RGATCY 3 cut(s) 198, 603, 736
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 6 cut(s) 209, 331, 359, 417, 518, 966
MluI ACGCGT 1 cut(s) 840
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 2 cut(s) 133, 629
MmeI TCCRAC 2 cut(s) 34, 663
MnlI CCTC 6 cut(s) 172, 311, 397, 487, 588, 825
Mox20I TGGCCA 1 cut(s) 413
MroXI GAANNNNTTC 2 cut(s) 192, 332
MscI TGGCCA 1 cut(s) 413
MseI TTAA 3 cut(s) 267, 582, 982
Msp20I TGGCCA 1 cut(s) 413
MspI CCGG 2 cut(s) 315, 617
MspR9I CCNGG 2 cut(s) 477, 932
MvaI CCWGG 2 cut(s) 477, 932
MvnI CGCG 2 cut(s) 500, 842
MwoI GCNNNNNNNGC 1 cut(s) 48
NcoI CCATGG 1 cut(s) 707
NdeII GATC 6 cut(s) 95, 198, 367, 375, 603, 736
NlaIII CATG 5 cut(s) 295, 340, 711, 745, 805
NspV TTCGAA 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 401
PagI TCATGA 2 cut(s) 741, 801
PdmI GAANNNNTTC 2 cut(s) 192, 332
PfeI GAWTC 4 cut(s) 24, 228, 295, 345
PleI GAGTC 2 cut(s) 133, 628
PpsI GAGTC 2 cut(s) 133, 628
Ppu21I YACGTR 1 cut(s) 283
Psp6I CCWGG 2 cut(s) 475, 930
PspGI CCWGG 2 cut(s) 475, 930
PspPI GGNCC 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 3 cut(s) 198, 603, 736
RsaI GTAC 2 cut(s) 290, 692
RsaNI GTAC 2 cut(s) 289, 691
SaqAI TTAA 3 cut(s) 267, 582, 982
Sau3AI GATC 6 cut(s) 95, 198, 367, 375, 603, 736
Sau96I GGNCC 1 cut(s) 340
SchI GAGTC 2 cut(s) 133, 629
ScrFI CCNGG 2 cut(s) 477, 932
SetI ASST 9 cut(s) 75, 88, 164, 285, 326, 809, 866, 900, 909
SfaNI GCATC 5 cut(s) 116, 160, 583, 775, 945
Sfr274I CTCGAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SnaBI TACGTA 1 cut(s) 283
Sse9I AATT 6 cut(s) 209, 331, 359, 417, 518, 966
SsiI CCGC 2 cut(s) 593, 767
SspMI CTAG 1 cut(s) 731
StyD4I CCNGG 2 cut(s) 475, 930
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 3 cut(s) 134, 154, 245
TaiI ACGT 1 cut(s) 285
TaqI TCGA 4 cut(s) 67, 402, 545, 874
TaqII GACCGA 1 cut(s) 357
TasI AATT 6 cut(s) 209, 331, 359, 417, 518, 966
TatI WGTACW 2 cut(s) 288, 690
TfiI GAWTC 4 cut(s) 24, 228, 295, 345
Tru1I TTAA 3 cut(s) 267, 582, 982
Tru9I TTAA 3 cut(s) 267, 582, 982
TscAI CASTG 2 cut(s) 157, 700
TspDTI ATGAA 5 cut(s) 17, 21, 325, 741, 758
TspGWI ACGGA 2 cut(s) 264, 616
TspRI CASTG 2 cut(s) 157, 700
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 2 cut(s) 192, 332
XspI CTAG 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.