AT2G06990

DSHCT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005703
Physical Location & Seq
Forward (+)
2894904 .. 2902020
7117 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G06990.1

Sequence Viewer

Length: 2988 bp
ATGAGTGCCCAAATGGAAGAACCAGAGACTCTCGGCAAAAGAAAAGAATCTGAAAGTTCAAAACTTCGTTCTGATGAAACTCCGACGCCAGAACCAAGAACGAAGCGTAGAAGTCTTAAACGTGCTTGTGTACATGAAGTTGCTGTTCCTAATGATTACACACCAACGAAAGAAGAGACGATTCATGGGACGCTTGATAATCCTGTCTTCAATGGAGACATGGCCAAGACTTATCCTTTTAAGCTTGACCCTTTTCAAAGTGTATCTGTAGCTTGTTTAGAGAGAAAAGAATCGATTTTGGTATCTGCTCATACTTCAGCTGGTAAAACTGCTGTTGCTGAGTATGCTATTGCAATGGCGTTTAGAGATAAGCAGAGAGTTATTTACACTTCTCCTTTGAAAGCTTTGAGTAATCAGAAGTATAGAGAGTTGCAACATGAGTTTAAAGATGTTGGTTTGATGACTGGTGATGTTACTCTTTCACCTAATGCTAGTTGTTTGGTTATGACTACTGAGATCTTGAGAGCTATGCTTTATAGAGGATCTGAAGTGTTAAAGGAGGTCGCTTGGGTTATTTTTGATGAGATTCATTATATGAAGGATAGAGAAAGAGGAGTTGTTTGGGAAGAGAGTATTATTTTCCTGCCTCCTGCTATTAAGATGGTATTCCTTTCTGCGACTATGTCGAATGCTACTGAATTTGCGGAATGGATATGTTATTTGCACAAGCAGCCATGTCATGTTGTGTACACAGATTTTAGGCCAACACCTCTGCAGCATTATGCTTTTCCTATGGGTGGGGGTGGACTGTACCTTGTGGTTGATGATAATGAGCAGTTTCGGGAGGATAGTTTCGTTAAGATGCAGGATACTTTCCCCAAACCGAAATCTAATGATGGGAAAAAGAGTGCGAATGGCAAATCTGGTGGTAGGGGTGCTAAAGGTGGAGGTGGCCCTGGTGATTCTGATGTTTACAAAATTGTAAAGATGATCATGGAGAGAAAATTTGAACCAGTCATTATCTTCAGCTTCAGTAGAAGAGAGTGTGAACAGCATGCATTGTCAATGTCAAAACTCGATTTTAATACAGATGAAGAGAAAGAAGTTGTGGAACAGGTGTTTAATAATGCAATGCAGTGCTTGAATGAAGAGGATAGATCCTTACCTGCCATTGAACTGATGTTGCCACTGCTCCAACGTGGTATTGCTGTCCATCATTCTGGTCTTCTTCCTGTCATCAAGGAATTAGTGGAGCTTCTTTTTCAGGAAGGACTTGTGAAAGCGCTTTTTGCCACAGAAACCTTTGCTATGGGTTTGAATATGCCCGCCAAAACTGTAGTGTTTACTGCTGTTAAGAAGTGGGATGGTGACAGCCATCGTTACATTGGGTCTGGTGAATATATCCAGATGAGCGGCAGGGCTGGACGTCGTGGAAAGGACGAGCGTGGCATCTGCATTATTATGATTGATGAACAGATGGAGATGAATACACTCAGGGACATGATGTTGGGAAAACCAGCCCCTTTGCTTAGTACTTTTAGGCTGAGTTACTACACAATTTTGAATCTATTGAGTCGTGCCGAAGGACAATTTACTGCTGAGCATGTTATAAGGCACTCGTTCCATCAATTCCAGCATGAAAAGGCTTTACCGGATATTGGGAATAAGGTGTCCAAACTTGAAGAGGAAGCTGCCATTCTTAACGCTTCTGGAGAGGCGGAGGTTGCTGAATACCATAATCTACAATTTGACATTGCTAAACATGAAAAGAAACTTATGTCAGAAATAATTAGGCCTGAAAGGGTGCTTTGTTTTCTTGATACTGGTCGGCTGGTTAAAATAAGAGAAGGTGGAACGGATTGGGGCTGGGGAGTTGTCGTTAATGTTGTAAAAAATTCTTCAGTTGGAACTGGTTCCGCCTCTTCACATGGTGGCGGATATATAGTGGATACTCTACTTCATTGCTCTACTGGTTTTAGTGAAAATGGCGCAAAGCCAAAGCCATGTCCTCCCCGTGCTGGGGAAAAGGGTGAAATGCATGTGGTTCCTGTTCAGCTACCCTTAATTTCTGCTTTGAGCAGACTAAGGATATCAGTTCCTTCTGATCTTCGGCCAGTGGAAGCAAGACAGAGTATACTACTTGCATTACAGGAGTTGTCAAGTCGCTTCCCTCTAGGGTTCCCAAAACTCCATCCAGTCAAGGATATGAATATTCAAGACACAGAGATAGTGGATCTGGTCAGCCAAATCGAAGAAGTTGAGCAGAAGTTACTTGCTCATCCGATGCACAAGTCTGAAGATGATCAACAAATCAAGAGTTTCCAGAGGAAAGCGGAGGTGAATTATGAAATTCAACAGCTAAAATCGAAAATGCGTGATTCCCAGCTCCAAAAGTTTCGAGATGAGCTTAAGAACCGATCTCGGGTATTAAAGAAACTTGGACACATTGATGCTGATGGTGTCGTCCAGGTAAAGGGACGAGCTGCGTGCTTGATAGACACAGGGGATGAATTGCTTGTCACTGAACTGATGTTTAATGGTACTTTTAATGATCTGGATCATCACCAAGTAGCAGCCCTTGCAAGCTGTTTCATTCCTGTTGATAAATCAAACGAACAGGTAAATTTAAGGAATGAACTTACCAAACCATTGCAGCAGCTCCAAGATAGTGCACGGAAAATAGCAGAGATACAACATGAATGCAAACTGGAAATTGACGTGGAAGAGTATGTGGAATCCACCATCAGGCCTTTCCTGATGGATGTTATCTACTCTTGGTCAAAGGGCGCAAGCTTTGCAGAGATTATACAAATGACTGACATATTCGAAGGTAGTATCATCAGAAGTGCTAGAAGACTCGACGAGTTTTTGAACCAGCTTCGAGCTGCAGCAGAGGCAGTTGGTGAAAGCAGTTTAGAGAGCAAGTTTGCAGCTGCTAGCGAGAGCTTGCGACGAGGTATTATGTTTGCAAATTCGCTTTACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

995

Amino Acids

111.89

Weight (kDa)

6.02

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 84 - 232 5.9e-26 DEAD/DEAH box helicase
DEXQc_Suv3 PF22527 99 - 215 1.1e-08 DEXQ-box helicase domain of Suv3
Helicase_C PF00271 399 - 478 3.7e-06 Helicase conserved C-terminal domain
MTR4-like_stalk PF21408 534 - 596 7.2e-15 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 599 - 793 1.8e-56 Mtr4-like, beta-barrel domain
DSHCT PF08148 819 - 990 8.7e-56 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1610
AatII GACGTC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 1174
AccBSI CCGCTC 1 cut(s) 1413
AccI GTMKAC 1 cut(s) 2134
AciI CCGC 7 cut(s) 704, 1326, 1413, 1718, 1917, 1935, 2333
AclWI GGATC 4 cut(s) 550, 1152, 2241, 2565
AcoI YGGCCR 2 cut(s) 222, 2111
AcsI RAATTY 6 cut(s) 698, 1004, 1894, 2349, 2623, 2971
AcuI CTGAAG 6 cut(s) 300, 567, 1009, 1015, 1884, 2316
AcyI GRCGYC 2 cut(s) 86, 1426
AdeI CACNNNGTG 1 cut(s) 1931
AfaI GTAC 5 cut(s) 132, 749, 812, 1534, 2542
AfeI AGCGCT 1 cut(s) 1284
AfiI CCNNNNNNNGG 8 cut(s) 797, 1658, 2018, 2019, 2020, 2422, 2473, 2745
AflII CTTAAG 1 cut(s) 2408
AjiI CACGTC 1 cut(s) 2719
AjnI CCWGG 2 cut(s) 955, 2466
Alw21I GWGCWC 1 cut(s) 2674
Alw26I GTCTC 3 cut(s) 20, 170, 210
Alw44I GTGCAC 1 cut(s) 2670
AlwI GGATC 4 cut(s) 550, 1152, 2241, 2565
Ama87I CYCGRG 1 cut(s) 2421
Aor51HI AGCGCT 1 cut(s) 1284
AoxI GGCC 6 cut(s) 222, 761, 952, 1793, 2111, 2747
ApaLI GTGCAC 1 cut(s) 2670
ApoI RAATTY 6 cut(s) 698, 1004, 1894, 2349, 2623, 2971
Asp700I GAANNNNTTC 1 cut(s) 1912
AspLEI GCGC 3 cut(s) 1285, 1991, 2789
AspS9I GGNCC 1 cut(s) 953
AsuHPI GGTGA 9 cut(s) 474, 479, 971, 1379, 1406, 2042, 2350, 2555, 2915
AsuII TTCGAA 1 cut(s) 2826
AsuNHI GCTAGC 1 cut(s) 2936
AvaI CYCGRG 1 cut(s) 2421
BaeGI GKGCMC 2 cut(s) 10, 2674
BalI TGGCCA 1 cut(s) 224
BbsI GAAGAC 3 cut(s) 199, 1217, 2860
Bbv12I GWGCWC 1 cut(s) 2674
BcgI CGANNNNNNTGC 2 cut(s) 2469, 2503
BciT130I CCWGG 2 cut(s) 957, 2468
BciVI GTATCC 2 cut(s) 862, 1942
BclI TGATCA 2 cut(s) 990, 2302
BcoDI GTCTC 3 cut(s) 20, 170, 210
BfaI CTAG 4 cut(s) 492, 2174, 2850, 2937
BfmI CTRYAG 4 cut(s) 267, 773, 1335, 2886
BfoI RGCGCY 1 cut(s) 1286
BfrI CTTAAG 1 cut(s) 2408
BfuAI ACCTGC 1 cut(s) 1174
BfuI GTATCC 2 cut(s) 862, 1942
BglII AGATCT 1 cut(s) 516
BlpI GCTNAGC 1 cut(s) 1599
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 2 cut(s) 957, 2468
BmeT110I CYCGRG 1 cut(s) 2421
BmgBI CACGTC 1 cut(s) 2719
BmgT120I GGNCC 1 cut(s) 953
BmiI GGNNCC 3 cut(s) 1915, 2046, 2180
BmrFI CCNGG 2 cut(s) 957, 2468
BmsI GCATC 4 cut(s) 852, 1458, 2274, 2440
BmtI GCTAGC 1 cut(s) 2940
BpiI GAAGAC 3 cut(s) 199, 1217, 2860
BpmI CTGGAG 1 cut(s) 1731
Bpu1102I GCTNAGC 1 cut(s) 1599
Bpu14I TTCGAA 1 cut(s) 2826
BpuEI CTTGAG 1 cut(s) 541
Bsa29I ATCGAT 1 cut(s) 293
BsaBI GATNNNNATC 1 cut(s) 2556
BsaHI GRCGYC 2 cut(s) 86, 1426
BsaJI CCNNGG 1 cut(s) 955
BsaWI WCCGGW 1 cut(s) 1651
BsaXI ACNNNNNCTCC 2 cut(s) 1863, 1893
Bsc4I CCNNNNNNNGG 8 cut(s) 797, 1658, 2018, 2019, 2020, 2422, 2473, 2745
Bse1I ACTGG 8 cut(s) 469, 1013, 1828, 1915, 1975, 2114, 2195, 2712
Bse3DI GCAATG 5 cut(s) 360, 1137, 1752, 1960, 2648
Bse8I GATNNNNATC 1 cut(s) 2556
BseBI CCWGG 2 cut(s) 957, 2468
BseCI ATCGAT 1 cut(s) 293
BseDI CCNNGG 1 cut(s) 955
BseGI GGATG 5 cut(s) 1369, 2191, 2278, 2512, 2767
BseJI GATNNNNATC 1 cut(s) 2556
BseLI CCNNNNNNNGG 8 cut(s) 797, 1658, 2018, 2019, 2020, 2422, 2473, 2745
BseMI GCAATG 5 cut(s) 360, 1137, 1752, 1960, 2648
BseMII CTCAG 5 cut(s) 330, 504, 1507, 1535, 1590
BseNI ACTGG 8 cut(s) 469, 1013, 1828, 1915, 1975, 2114, 2195, 2712
BseRI GAGGAG 1 cut(s) 627
BseSI GKGCMC 2 cut(s) 10, 2674
BseYI CCCAGC 3 cut(s) 1866, 2018, 2382
BshFI GGCC 6 cut(s) 224, 763, 954, 1795, 2113, 2749
BshVI ATCGAT 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 2674
BsiHKCI CYCGRG 1 cut(s) 2421
BsiSI CCGG 1 cut(s) 1652
BslFI GGGAC 3 cut(s) 202, 1511, 2490
BslI CCNNNNNNNGG 8 cut(s) 797, 1658, 2018, 2019, 2020, 2422, 2473, 2745
BsmAI GTCTC 3 cut(s) 20, 170, 210
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 3 cut(s) 202, 1511, 2490
BsmI GAATGC 2 cut(s) 694, 2705
BsnI GGCC 6 cut(s) 224, 763, 954, 1795, 2113, 2749
BsoBI CYCGRG 1 cut(s) 2421
Bsp119I TTCGAA 1 cut(s) 2826
Bsp1286I GDGCHC 2 cut(s) 10, 2674
Bsp1407I TGTACA 2 cut(s) 130, 747
Bsp1720I GCTNAGC 1 cut(s) 1599
BspACI CCGC 7 cut(s) 704, 1326, 1413, 1718, 1917, 1935, 2333
BspANI GGCC 6 cut(s) 224, 763, 954, 1795, 2113, 2749
BspCNI CTCAG 5 cut(s) 331, 505, 1506, 1536, 1591
BspDI ATCGAT 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 1915, 2046, 2180
BspMAI CTGCAG 2 cut(s) 777, 2890
BspMI ACCTGC 1 cut(s) 1174
BspOI GCTAGC 1 cut(s) 2940
BspPI GGATC 4 cut(s) 550, 1152, 2241, 2565
BspT104I TTCGAA 1 cut(s) 2826
BspTI CTTAAG 1 cut(s) 2408
BsrBI CCGCTC 1 cut(s) 1413
BsrDI GCAATG 5 cut(s) 360, 1137, 1752, 1960, 2648
BsrGI TGTACA 2 cut(s) 130, 747
BsrI ACTGG 8 cut(s) 469, 1013, 1828, 1915, 1975, 2114, 2195, 2712
BssECI CCNNGG 1 cut(s) 955
BssNAI GTATAC 1 cut(s) 2135
BssNI GRCGYC 2 cut(s) 86, 1426
Bst1107I GTATAC 1 cut(s) 2135
Bst2UI CCWGG 2 cut(s) 957, 2468
Bst4CI ACNGT 2 cut(s) 810, 1336
Bst6I CTCTTC 8 cut(s) 168, 621, 1033, 1089, 1143, 1677, 1927, 2718
BstACI GRCGYC 2 cut(s) 86, 1426
BstAFI CTTAAG 1 cut(s) 2408
BstAPI GCANNNNNTGC 2 cut(s) 2579, 2795
BstAUI TGTACA 2 cut(s) 130, 747
BstBI TTCGAA 1 cut(s) 2826
BstC8I GCNNGC 7 cut(s) 1056, 1326, 2488, 2584, 2791, 2938, 2948
BstDEI CTNAG 7 cut(s) 339, 513, 1493, 1529, 1544, 1599, 2084
BstF5I GGATG 5 cut(s) 1369, 2191, 2278, 2512, 2767
BstH2I RGCGCY 1 cut(s) 1286
BstHHI GCGC 3 cut(s) 1285, 1991, 2789
BstMAI GTCTC 3 cut(s) 20, 170, 210
BstMWI GCNNNNNNNGC 7 cut(s) 344, 730, 1289, 1724, 2579, 2795, 2894
BstNI CCWGG 2 cut(s) 957, 2468
BstNSI RCATGY 3 cut(s) 1058, 1607, 2042
BstSCI CCNGG 2 cut(s) 955, 2466
BstSFI CTRYAG 4 cut(s) 267, 773, 1335, 2886
BstSLI GKGCMC 2 cut(s) 10, 2674
BstV2I GAAGAC 3 cut(s) 199, 1217, 2860
BstX2I RGATCY 4 cut(s) 516, 542, 1157, 2233
BstXI CCANNNNNNTGG 1 cut(s) 1220
BstYI RGATCY 4 cut(s) 516, 542, 1157, 2233
BstZ17I GTATAC 1 cut(s) 2135
Bsu15I ATCGAT 1 cut(s) 293
BsuI GTATCC 2 cut(s) 862, 1942
BsuRI GGCC 6 cut(s) 224, 763, 954, 1795, 2113, 2749
BsuTUI ATCGAT 1 cut(s) 293
BtrI CACGTC 1 cut(s) 2719
BtsCI GGATG 5 cut(s) 1369, 2191, 2278, 2512, 2767
BtsI GCAGTG 2 cut(s) 1142, 1187
BtsIMutI CAGTG 4 cut(s) 1142, 1187, 2121, 2520
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 7 cut(s) 1056, 1326, 2488, 2584, 2791, 2938, 2948
CfoI GCGC 3 cut(s) 1285, 1991, 2789
Cfr13I GGNCC 1 cut(s) 953
ClaI ATCGAT 1 cut(s) 293
CseI GACGC 2 cut(s) 94, 199
Csp6I GTAC 5 cut(s) 131, 748, 811, 1533, 2541
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviQI GTAC 5 cut(s) 131, 748, 811, 1533, 2541
DdeI CTNAG 7 cut(s) 339, 513, 1493, 1529, 1544, 1599, 2084
DraI TTTAAA 1 cut(s) 445
DraIII CACNNNGTG 1 cut(s) 1931
EaeI YGGCCR 2 cut(s) 222, 2111
Eam1104I CTCTTC 8 cut(s) 168, 621, 1033, 1089, 1143, 1677, 1927, 2718
EarI CTCTTC 8 cut(s) 168, 621, 1033, 1089, 1143, 1677, 1927, 2718
EciI GGCGGA 3 cut(s) 1733, 1906, 1950
Eco147I AGGCCT 2 cut(s) 1795, 2749
Eco32I GATATC 1 cut(s) 2091
Eco47III AGCGCT 1 cut(s) 1284
Eco57I CTGAAG 6 cut(s) 300, 567, 1009, 1015, 1884, 2316
Eco88I CYCGRG 1 cut(s) 2421
EcoRII CCWGG 2 cut(s) 955, 2466
EcoRV GATATC 1 cut(s) 2091
EcoT22I ATGCAT 2 cut(s) 1060, 2040
Esp3I CGTCTC 1 cut(s) 170
FaqI GGGAC 3 cut(s) 202, 1511, 2490
FauI CCCGC 1 cut(s) 1333
FbaI TGATCA 2 cut(s) 990, 2302
FblI GTMKAC 1 cut(s) 2134
FokI GGATG 5 cut(s) 1376, 2178, 2265, 2519, 2774
FspBI CTAG 4 cut(s) 492, 2174, 2850, 2937
GlaI GCGC 3 cut(s) 1284, 1990, 2788
GsaI CCCAGC 3 cut(s) 1870, 2022, 2386
GsuI CTGGAG 1 cut(s) 1731
HaeII RGCGCY 1 cut(s) 1286
HaeIII GGCC 6 cut(s) 224, 763, 954, 1795, 2113, 2749
HapII CCGG 1 cut(s) 1652
HgaI GACGC 2 cut(s) 94, 199
HhaI GCGC 3 cut(s) 1285, 1991, 2789
Hin1I GRCGYC 2 cut(s) 86, 1426
Hin6I GCGC 3 cut(s) 1283, 1989, 2787
HinP1I GCGC 3 cut(s) 1283, 1989, 2787
HindIII AAGCTT 3 cut(s) 242, 402, 2791
HpaII CCGG 1 cut(s) 1652
HphI GGTGA 9 cut(s) 474, 479, 971, 1379, 1406, 2042, 2350, 2555, 2915
Hpy166II GTNNAC 9 cut(s) 131, 748, 750, 806, 973, 1049, 1344, 2135, 2672
Hpy8I GTNNAC 9 cut(s) 131, 748, 750, 806, 973, 1049, 1344, 2135, 2672
Hpy99I CGWCG 4 cut(s) 88, 1431, 2864, 2955
HpyAV CCTTC 6 cut(s) 592, 1262, 1577, 1841, 2109, 2822
HpyCH4III ACNGT 2 cut(s) 810, 1336
HpyCH4IV ACGT 4 cut(s) 121, 1198, 1426, 2718
HpyF10VI GCNNNNNNNGC 7 cut(s) 344, 730, 1289, 1724, 2579, 2795, 2894
HpyF3I CTNAG 7 cut(s) 339, 513, 1493, 1529, 1544, 1599, 2084
HpySE526I ACGT 4 cut(s) 121, 1198, 1426, 2718
Hsp92I GRCGYC 2 cut(s) 86, 1426
HspAI GCGC 3 cut(s) 1283, 1989, 2787
Ksp22I TGATCA 2 cut(s) 990, 2302
LmnI GCTCC 4 cut(s) 1197, 1252, 2391, 2664
LweI GCATC 4 cut(s) 852, 1458, 2274, 2440
MaeI CTAG 4 cut(s) 492, 2174, 2850, 2937
MaeII ACGT 4 cut(s) 121, 1198, 1426, 2718
MaeIII GTNAC 6 cut(s) 472, 1367, 1379, 1547, 2268, 2518
MbiI CCGCTC 1 cut(s) 1413
MflI RGATCY 4 cut(s) 516, 542, 1157, 2233
MhlI GDGCHC 2 cut(s) 10, 2674
MlsI TGGCCA 1 cut(s) 224
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 3 cut(s) 22, 1582, 2850
MmeI TCCRAC 3 cut(s) 107, 1219, 1885
Mox20I TGGCCA 1 cut(s) 224
Mph1103I ATGCAT 2 cut(s) 1060, 2040
MroXI GAANNNNTTC 1 cut(s) 1912
MscI TGGCCA 1 cut(s) 224
MslI CAYNNNNRTG 2 cut(s) 1460, 2448
Msp20I TGGCCA 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 320, 2933
MspCI CTTAAG 1 cut(s) 2408
MspI CCGG 1 cut(s) 1652
MspR9I CCNGG 2 cut(s) 957, 2468
Mva1269I GAATGC 2 cut(s) 694, 2705
MvaI CCWGG 2 cut(s) 957, 2468
MwoI GCNNNNNNNGC 7 cut(s) 344, 730, 1289, 1724, 2579, 2795, 2894
NheI GCTAGC 1 cut(s) 2936
NlaIV GGNNCC 3 cut(s) 1915, 2046, 2180
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 2 cut(s) 1367, 2518
NsiI ATGCAT 2 cut(s) 1060, 2040
NspI RCATGY 3 cut(s) 1058, 1607, 2042
NspV TTCGAA 1 cut(s) 2826
PaeI GCATGC 1 cut(s) 1058
PceI AGGCCT 2 cut(s) 1795, 2749
PctI GAATGC 2 cut(s) 694, 2705
PdmI GAANNNNTTC 1 cut(s) 1912
PfeI GAWTC 8 cut(s) 47, 181, 290, 586, 962, 1564, 2378, 2735
PflFI GACNNNGTC 1 cut(s) 682
PleI GAGTC 3 cut(s) 22, 1581, 2850
PpsI GAGTC 3 cut(s) 22, 1581, 2850
PsiI TTATAA 1 cut(s) 1610
Psp6I CCWGG 2 cut(s) 955, 2466
PspFI CCCAGC 3 cut(s) 1866, 2018, 2382
PspGI CCWGG 2 cut(s) 955, 2466
PspN4I GGNNCC 3 cut(s) 1915, 2046, 2180
PspPI GGNCC 1 cut(s) 953
PstI CTGCAG 2 cut(s) 777, 2890
PsuI RGATCY 4 cut(s) 516, 542, 1157, 2233
PsyI GACNNNGTC 1 cut(s) 682
PvuII CAGCTG 2 cut(s) 320, 2933
RsaI GTAC 5 cut(s) 132, 749, 812, 1534, 2542
RsaNI GTAC 5 cut(s) 131, 748, 811, 1533, 2541
RseI CAYNNNNRTG 2 cut(s) 1460, 2448
Sau96I GGNCC 1 cut(s) 953
ScaI AGTACT 1 cut(s) 1534
SchI GAGTC 3 cut(s) 22, 1582, 2850
ScrFI CCNGG 2 cut(s) 957, 2468
SduI GDGCHC 2 cut(s) 10, 2674
SfaNI GCATC 4 cut(s) 852, 1458, 2274, 2440
SfcI CTRYAG 4 cut(s) 267, 773, 1335, 2886
SfuI TTCGAA 1 cut(s) 2826
SmiMI CAYNNNNRTG 2 cut(s) 1460, 2448
SmlI CTYRAG 2 cut(s) 520, 2408
SmoI CTYRAG 2 cut(s) 520, 2408
SphI GCATGC 1 cut(s) 1058
SseBI AGGCCT 2 cut(s) 1795, 2749
SsiI CCGC 7 cut(s) 704, 1326, 1413, 1718, 1917, 1935, 2333
SspI AATATT 1 cut(s) 2212
SspMI CTAG 4 cut(s) 492, 2174, 2850, 2937
StuI AGGCCT 2 cut(s) 1795, 2749
StyD4I CCNGG 2 cut(s) 955, 2466
TaaI ACNGT 2 cut(s) 810, 1336
TaiI ACGT 4 cut(s) 124, 1201, 1429, 2721
TaqI TCGA 9 cut(s) 293, 686, 1077, 2250, 2366, 2398, 2826, 2859, 2881
TatI WGTACW 3 cut(s) 130, 747, 1532
TauI GCSGC 1 cut(s) 1416
TfiI GAWTC 8 cut(s) 47, 181, 290, 586, 962, 1564, 2378, 2735
TscAI CASTG 4 cut(s) 1142, 1194, 2121, 2527
TseFI GTSAC 2 cut(s) 1367, 2518
Tsp45I GTSAC 2 cut(s) 1367, 2518
TspGWI ACGGA 2 cut(s) 1871, 2689
TspRI CASTG 4 cut(s) 1142, 1194, 2121, 2527
Tth111I GACNNNGTC 1 cut(s) 682
Vha464I CTTAAG 1 cut(s) 2408
VneI GTGCAC 1 cut(s) 2670
XapI RAATTY 6 cut(s) 698, 1004, 1894, 2349, 2623, 2971
XceI RCATGY 3 cut(s) 1058, 1607, 2042
XcmI CCANNNNNNNNNTGG 1 cut(s) 1382
XmiI GTMKAC 1 cut(s) 2134
XmnI GAANNNNTTC 1 cut(s) 1912
XspI CTAG 4 cut(s) 492, 2174, 2850, 2937
ZraI GACGTC 1 cut(s) 1427
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 2 cut(s) 1060, 2040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.