MD16G1238700.v1.1

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005703
Physical Location & Seq
Reverse (-)
25220694 .. 25229053
8360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1238700.v1.1.491

Sequence Viewer

Length: 2907 bp
ATGGAAGAATCTCCAACACCTGCGAAACGAAAAGAACGTGAAGCGTCAGAGATCACAGAGACTCCCACCGACGAATCTTCCCTGAAGCGACGGCATTTGACTCGAACATGTGTGCATGAGGTTGCAGTTCCCAGTGACTATGCTTCGACCAAGGATGAGTCGGTTTATGGAACCCTTTCGAATCCTGTTTATAATGGCGAGGCGGCCAAGACATACCAATTTACCCTTGACCCATTTCAGCAGATTTCTGTGGCATGCTTAGAACGGAAAGAGTCAGTTTTGGTCTCCGCCCATACTTCAGCAGGTAAGACTGCAGTCGCGGAGTATGCAATTGCCATGGCTTTTAGAGACAAGCAGAGGGTTATATATACTTCGCCATTGAAGGCTTTGAGCAATCAGAAATATAGAGAGCTCAGCCAGGAGTTTGAAGATGTTGGATTGATGACTGGAGATGTTACACTCTCACCTAATGCTAGTTGTTTGGTTATGACAACTGAGATCCTTAGGGGAATGCTTTACAGAGGTTCCGAGGTTTTAAAGGAAGTCGCCTGGGTTATCTTCGATGAAATTCACTACATGAAGGATCGTGAAAGAGGGGTTGTTTGGGAAGAGAGTATTATATTTATGCCTCCCGCTGTTAAAATGGTTTTTCTTTCAGCCACAATGTCGAATGCGACTGAGTTTGCAGAGTGGATTTGTAATTTGCACAAACAGCCTTGTCATGTGGTGTACACAGATTTTCGACCAACTCCTCTGCAGCATTACGTTTTTCCAGTGGGTGGGAATGGGTTGTATCTTGTGGTCGATGAGAATGAACAATTCAGGGAGGAAAATTTTGTGAAGCTACATGATACTTTCTCAAAGCAGAAATTGAGTGATGGTCACAGGAATAACAAGGCAAGTGGGAGACTTGCAAAAGGTGGGACTGCATCTGGGGGTTCTGACATATTCAAAATTGTGAAGATGATTATGGAACGGAAATTTCAGCCAGTTATAATTTTCAGCTTCAGTAGAAGAGAGTGTGAACAACATGCGATGTCCATGTCTAAGCTTGATTTTAACAGCCAAGATGAAAAAGATGCTGTTGAACAGGTTTTTCGTAATGCAATACTCTGCTTAAATGAGGAAGACAGGGAGTTGCCTGCTATTGAGTTAATGCTGCCACTCCTTCAACGAGGAATTGCCGTTCATCATTCTGGCCTTCTACCTGTTATCAAGGAACTGGTAGAACTTCTTTTCCAAGAAGGCCTTGTAAAAGCCCTTTTTGCTACTGAGACATGGGATGGTGATAGTCATCGATATATCGGATCTGGTGAGTATATTCAGATGAGTGGAAGGGCTGGGCGTCGTGGCAAAGATGAGCGGGGTATTTGTATCATAATGATTGATGAACAGATGGAAATGAACACACTAAAGGATATGGTTTTGGGTAAACCAGCTCCTCTAGTTAGCACTTTCAGACTCAGCTATTACTCAATTTTGAATTTGTTGAGCCGTGCGGAAGGCCAATTCACAGCTGAACACGTGATAAAGAATTCATTCCACCAATTTCAGTATGAAAAGGCTTTGCCTGGTATAGGGGAAAAGGTTTCCAAGCTTGAGCAGGAAGCGGCTTTGCTTGATGCATCAGGAGAGGCTGAAGTTGCTGAGTATCATAAGATAAAACTTGATATAGCTCAACTTGAGAAGAAAATGATGTCAGAGATAACGAGGCCTGAAAGAGTTCTGTATTTTCTTCTTCCTGGTCGCCTGGTCAAGATTAGGGAAGGTGGTACAGATTGGGGCTGGGGAGTTGTAGTTAATGTGGTAAAAAAGCCTTCATCCGGGTTAGGCACACTGTCCTCGCGCGGTGGTGGGTTTATAGTGGATACTTTGCTCCACTGTTCTCCTGGTTCAAGTGAGAACAGTTCACAGCCAAAGCCTTGCCCTCCTCGCCCTGGAGAAAAGGGTGAAATGCATGTAGTCCCTGTACAATTGCCCCTGATTTCTGCTCTAAGCAAACTCAGAATATCTATTCCCTCTGATTTGCGGCCGTTAGAGGCGAGGCAAAGTATTCTTCTAGCAGTTCAGGAGCTGGGAACTCGATTCCCACAAGGTCTTCCAAAGCTGAATCCTGTAAAGGATATGGGCATTGAAGATCCTGAAATTGTTGAGTTAGTCAATCAAATAGAGGATCTTGAACAGAAATTATATGCACATCCACTGCATAAGTCTCAAGATGTGAATCAGATTAAATGCTTTCAAAGGAAAGCAGAGGTGGATCATGAAATTCAACAACTTAAGTCCAAAATGCGTGAGTCCCAGCTTCAAAAATTTCGTGATGAACTTAAGAACCGTTCACGGGTTTTGAAGAAGCTTGGTCACATTGATGCCGAGGGTGTTGTCCAGTTAAAGGGGCGGGCAGCCTGCTTGATAGACACAGGAGATGAGCTCCTTGTTACTGAACTGATGTTTAATGGTACATTCAACGATTTGGATCATCATCAAATTGCAGCAATCGCAAGTTGTTTTATTCCAGTAGATAAATCAAATGAGCAGATACAATTGAGGACGGAGCTTGCTAGACCATTACAACAACTCCAAGAGAGTGCTAGAAGAATAGCAGAGATACAACATGAATGCAAATTAGATGTAAACATTGAAGAATACGTGGAATCAACAGTGCGACCGTTCTTGATGGATGTGATTTATTGTTGGTCAAAGGGTGCAAGTTTCGCGGAGGTTACACAAATGACCGATATATTTGAGGGTAGTATCATCAGAGCTGCTAGAAGACTAGATGAATTTTTGAACCAGTTGCGTACTGCTGCTCAGGCTGTGGGAGAAGTTGCTTTGGAAGAGAAATTCACTGGAGCAAGCGAAAGCCTCCGGCGAGGTATCATGTTTGCAAATTCCCTGTATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

969

Amino Acids

109.1

Weight (kDa)

5.87

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 78 - 226 2.8e-26 DEAD/DEAH box helicase
DEXQc_Suv3 PF22527 93 - 210 1.9e-08 DEXQ-box helicase domain of Suv3
MTR4-like_stalk PF21408 507 - 570 4.6e-16 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 572 - 766 2.4e-57 Mtr4-like, beta-barrel domain
DSHCT PF08148 793 - 963 1.7e-55 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 192, 995
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 2 cut(s) 28, 293
AccB7I CCANNNNNTGG 1 cut(s) 779
AccBSI CCGCTC 1 cut(s) 1363
AccII CGCG 4 cut(s) 320, 1846, 1848, 2717
AclWI GGATC 7 cut(s) 493, 591, 1315, 2132, 2181, 2268, 2484
AcoI YGGCCR 2 cut(s) 204, 2030
AcuI CTGAAG 4 cut(s) 104, 282, 991, 1659
AcvI CACGTG 1 cut(s) 1525
AcyI GRCGYC 1 cut(s) 1345
AfaI GTAC 5 cut(s) 731, 1774, 1971, 2461, 2803
AfiI CCNNNNNNNGG 6 cut(s) 779, 1577, 1823, 1937, 2341, 2392
AflII CTTAAG 2 cut(s) 2279, 2327
AflIII ACRYGT 2 cut(s) 107, 1522
AjnI CCWGG 7 cut(s) 417, 548, 1570, 1741, 1749, 1888, 1936
AloI GAACNNNNNNTCC 4 cut(s) 1170, 1202, 1221, 1253
Alw21I GWGCWC 2 cut(s) 414, 2433
Alw26I GTCTC 6 cut(s) 53, 289, 342, 901, 1268, 2217
AlwI GGATC 7 cut(s) 493, 591, 1315, 2132, 2181, 2268, 2484
AlwNI CAGNNNCTG 1 cut(s) 2074
AoxI GGCC 6 cut(s) 204, 1198, 1246, 1504, 1712, 2030
ApeKI GCWGC 6 cut(s) 757, 1159, 2402, 2492, 2765, 2807
Asp700I GAANNNNTTC 3 cut(s) 175, 1538, 1722
AspLEI GCGC 1 cut(s) 1848
AsuC2I CCSGG 1 cut(s) 1825
AsuHPI GGTGA 4 cut(s) 456, 1298, 1325, 1961
AsuII TTCGAA 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 503
BanII GRGCYC 2 cut(s) 414, 2433
BbrPI CACGTG 1 cut(s) 1525
BbsI GAAGAC 3 cut(s) 1134, 2090, 2779
Bbv12I GWGCWC 2 cut(s) 414, 2433
BbvI GCAGC 6 cut(s) 769, 1146, 2414, 2504, 2752, 2794
BccI CCATC 4 cut(s) 872, 1277, 1390, 2671
BceAI ACGGC 4 cut(s) 107, 1169, 1479, 2017
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 7 cut(s) 419, 550, 1572, 1743, 1751, 1890, 1938
BciVI GTATCC 1 cut(s) 1861
BcnI CCSGG 1 cut(s) 1825
BcoDI GTCTC 6 cut(s) 53, 289, 342, 901, 1268, 2217
BfaI CTAG 7 cut(s) 474, 1445, 2060, 2562, 2592, 2769, 2777
BfmI CTRYAG 3 cut(s) 312, 755, 2903
BfrI CTTAAG 2 cut(s) 2279, 2327
BfuAI ACCTGC 2 cut(s) 28, 293
BfuI GTATCC 1 cut(s) 1861
BisI GCNGC 9 cut(s) 204, 758, 1160, 1611, 2030, 2403, 2493, 2766, 2808
BlpI GCTNAGC 1 cut(s) 413
BlsI GCNGC 9 cut(s) 205, 759, 1161, 1612, 2031, 2404, 2494, 2767, 2809
Bme1390I CCNGG 8 cut(s) 419, 550, 1572, 1743, 1751, 1825, 1890, 1938
BmiI GGNNCC 2 cut(s) 172, 526
BmrFI CCNGG 8 cut(s) 419, 550, 1572, 1743, 1751, 1825, 1890, 1938
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 5 cut(s) 938, 1069, 1612, 1634, 2359
BmuI ACTGGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 3 cut(s) 1134, 2090, 2779
BplI GAGNNNNNCTC 2 cut(s) 2415, 2447
BpmI CTGGAG 3 cut(s) 468, 1959, 2871
Bpu10I CCTNAGC 1 cut(s) 2811
Bpu1102I GCTNAGC 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 3 cut(s) 1619, 1703, 2199
BpuMI CCSGG 1 cut(s) 1825
Bsa29I ATCGAT 1 cut(s) 1297
BsaAI YACGTR 2 cut(s) 1525, 2650
BsaBI GATNNNNATC 4 cut(s) 1293, 2223, 2475, 2481
BsaHI GRCGYC 1 cut(s) 1345
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 6 cut(s) 150, 336, 528, 549, 1936, 2373
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 1782, 1812, 2562, 2592
Bsc4I CCNNNNNNNGG 6 cut(s) 779, 1577, 1823, 1937, 2341, 2392
Bse1I ACTGG 9 cut(s) 132, 451, 773, 989, 1227, 2386, 2516, 2794, 2854
Bse21I CCTNAGG 1 cut(s) 503
Bse8I GATNNNNATC 4 cut(s) 1293, 2223, 2475, 2481
BseBI CCWGG 7 cut(s) 419, 550, 1572, 1743, 1751, 1890, 1938
BseCI ATCGAT 1 cut(s) 1297
BseDI CCNNGG 6 cut(s) 150, 336, 528, 549, 1936, 2373
BseGI GGATG 5 cut(s) 160, 1288, 1820, 2197, 2686
BseJI GATNNNNATC 4 cut(s) 1293, 2223, 2475, 2481
BseLI CCNNNNNNNGG 6 cut(s) 779, 1577, 1823, 1937, 2341, 2392
BseMII CTCAG 8 cut(s) 427, 486, 669, 1263, 1477, 1638, 2017, 2825
BseNI ACTGG 9 cut(s) 132, 451, 773, 989, 1227, 2386, 2516, 2794, 2854
BseRI GAGGAG 3 cut(s) 741, 1431, 1920
BseX3I CGGCCG 1 cut(s) 2030
BseXI GCAGC 6 cut(s) 769, 1146, 2414, 2504, 2752, 2794
BseYI CCCAGC 4 cut(s) 1340, 1785, 2074, 2301
Bsh1236I CGCG 4 cut(s) 320, 1846, 1848, 2717
Bsh1285I CGRYCG 2 cut(s) 2033, 2669
BshFI GGCC 6 cut(s) 206, 1200, 1248, 1506, 1714, 2032
BshVI ATCGAT 1 cut(s) 1297
BsiEI CGRYCG 2 cut(s) 2033, 2669
BsiHKAI GWGCWC 2 cut(s) 414, 2433
BsiSI CCGG 2 cut(s) 1824, 2869
BslFI GGGAC 3 cut(s) 937, 1949, 2284
BslI CCNNNNNNNGG 6 cut(s) 779, 1577, 1823, 1937, 2341, 2392
BsmAI GTCTC 6 cut(s) 53, 289, 342, 901, 1268, 2217
BsmFI GGGAC 3 cut(s) 937, 1949, 2284
BsmI GAATGC 3 cut(s) 516, 676, 2624
BsnI GGCC 6 cut(s) 206, 1200, 1248, 1506, 1714, 2032
Bso31I GGTCTC 1 cut(s) 289
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 414, 2433
Bsp1407I TGTACA 2 cut(s) 729, 1969
Bsp143I GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
Bsp1720I GCTNAGC 1 cut(s) 413
Bsp19I CCATGG 1 cut(s) 336
BspANI GGCC 6 cut(s) 206, 1200, 1248, 1506, 1714, 2032
BspCNI CTCAG 8 cut(s) 426, 487, 670, 1264, 1476, 1639, 2016, 2824
BspDI ATCGAT 1 cut(s) 1297
BspFNI CGCG 4 cut(s) 320, 1846, 1848, 2717
BspHI TCATGA 1 cut(s) 2263
BspLI GGNNCC 2 cut(s) 172, 526
BspMAI CTGCAG 2 cut(s) 316, 759
BspMI ACCTGC 2 cut(s) 28, 293
BspPI GGATC 7 cut(s) 493, 591, 1315, 2132, 2181, 2268, 2484
BspT104I TTCGAA 1 cut(s) 179
BspTI CTTAAG 2 cut(s) 2279, 2327
BspTNI GGTCTC 1 cut(s) 289
BsrBI CCGCTC 1 cut(s) 1363
BsrGI TGTACA 2 cut(s) 729, 1969
BsrI ACTGG 9 cut(s) 132, 451, 773, 989, 1227, 2386, 2516, 2794, 2854
BssECI CCNNGG 6 cut(s) 150, 336, 528, 549, 1936, 2373
BssMI GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
BssNI GRCGYC 1 cut(s) 1345
BssT1I CCWWGG 2 cut(s) 150, 336
Bst2UI CCWGG 7 cut(s) 419, 550, 1572, 1743, 1751, 1890, 1938
Bst4CI ACNGT 6 cut(s) 1839, 1883, 1907, 2336, 2662, 2670
Bst6I CTCTTC 3 cut(s) 603, 1009, 2832
BstACI GRCGYC 1 cut(s) 1345
BstAFI CTTAAG 2 cut(s) 2279, 2327
BstAUI TGTACA 2 cut(s) 729, 1969
BstBAI YACGTR 2 cut(s) 1525, 2650
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 6 cut(s) 256, 1143, 2400, 2407, 2559, 2857
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 1575
BstF5I GGATG 5 cut(s) 160, 1288, 1820, 2197, 2686
BstFNI CGCG 4 cut(s) 320, 1846, 1848, 2717
BstHHI GCGC 1 cut(s) 1848
BstKTI GATC 8 cut(s) 54, 501, 586, 1310, 2140, 2176, 2263, 2479
BstMAI GTCTC 6 cut(s) 53, 289, 342, 901, 1268, 2217
BstMBI GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
BstMCI CGRYCG 2 cut(s) 2033, 2669
BstMWI GCNNNNNNNGC 8 cut(s) 326, 712, 1265, 1643, 1932, 2498, 2714, 2813
BstNI CCWGG 7 cut(s) 419, 550, 1572, 1743, 1751, 1890, 1938
BstNSI RCATGY 4 cut(s) 111, 258, 1034, 1961
BstPAI GACNNNNGTC 1 cut(s) 314
BstSCI CCNGG 8 cut(s) 417, 548, 1570, 1741, 1749, 1823, 1888, 1936
BstSFI CTRYAG 3 cut(s) 312, 755, 2903
BstUI CGCG 4 cut(s) 320, 1846, 1848, 2717
BstV1I GCAGC 6 cut(s) 769, 1146, 2414, 2504, 2752, 2794
BstV2I GAAGAC 3 cut(s) 1134, 2090, 2779
BstX2I RGATCY 4 cut(s) 498, 1307, 2137, 2173
BstYI RGATCY 4 cut(s) 498, 1307, 2137, 2173
BstZI CGGCCG 1 cut(s) 2030
Bsu15I ATCGAT 1 cut(s) 1297
Bsu36I CCTNAGG 1 cut(s) 503
BsuI GTATCC 1 cut(s) 1861
BsuRI GGCC 6 cut(s) 206, 1200, 1248, 1506, 1714, 2032
BsuTUI ATCGAT 1 cut(s) 1297
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 1049
BtsCI GGATG 5 cut(s) 160, 1288, 1820, 2197, 2686
BtsI GCAGTG 1 cut(s) 2201
BtsIMutI CAGTG 7 cut(s) 139, 780, 1835, 1879, 2201, 2667, 2847
BveI ACCTGC 2 cut(s) 28, 293
Cac8I GCNNGC 6 cut(s) 256, 1143, 2400, 2407, 2559, 2857
CaiI CAGNNNCTG 1 cut(s) 2074
CciI TCATGA 1 cut(s) 2263
CfoI GCGC 1 cut(s) 1848
ClaI ATCGAT 1 cut(s) 1297
CseI GACGC 2 cut(s) 33, 1334
Csp6I GTAC 5 cut(s) 730, 1773, 1970, 2460, 2802
CspCI CAANNNNNGTGG 2 cut(s) 883, 918
CviQI GTAC 5 cut(s) 730, 1773, 1970, 2460, 2802
DpnI GATC 8 cut(s) 53, 500, 585, 1309, 2139, 2175, 2262, 2478
DpnII GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
DraI TTTAAA 1 cut(s) 537
EaeI YGGCCR 2 cut(s) 204, 2030
EagI CGGCCG 1 cut(s) 2030
Eam1104I CTCTTC 3 cut(s) 603, 1009, 2832
EarI CTCTTC 3 cut(s) 603, 1009, 2832
EciI GGCGGA 1 cut(s) 277
Ecl136II GAGCTC 2 cut(s) 412, 2431
EclXI CGGCCG 1 cut(s) 2030
Eco130I CCWWGG 2 cut(s) 150, 336
Eco147I AGGCCT 2 cut(s) 1248, 1714
Eco24I GRGCYC 2 cut(s) 414, 2433
Eco31I GGTCTC 1 cut(s) 289
Eco52I CGGCCG 1 cut(s) 2030
Eco53kI GAGCTC 2 cut(s) 412, 2431
Eco57I CTGAAG 4 cut(s) 104, 282, 991, 1659
Eco72I CACGTG 1 cut(s) 1525
Eco81I CCTNAGG 1 cut(s) 503
EcoICRI GAGCTC 2 cut(s) 412, 2431
EcoNI CCTNNNNNAGG 1 cut(s) 1575
EcoRI GAATTC 1 cut(s) 1534
EcoRII CCWGG 7 cut(s) 417, 548, 1570, 1741, 1749, 1888, 1936
EcoT14I CCWWGG 2 cut(s) 150, 336
EcoT22I ATGCAT 2 cut(s) 1627, 1959
EcoT38I GRGCYC 2 cut(s) 414, 2433
ErhI CCWWGG 2 cut(s) 150, 336
FalI AAGNNNNNCTT 2 cut(s) 1233, 1265
FaqI GGGAC 3 cut(s) 937, 1949, 2284
FauI CCCGC 3 cut(s) 640, 1356, 2391
Fnu4HI GCNGC 9 cut(s) 204, 758, 1160, 1611, 2030, 2403, 2493, 2766, 2808
FokI GGATG 5 cut(s) 167, 1295, 1807, 2184, 2693
FriOI GRGCYC 2 cut(s) 414, 2433
Fsp4HI GCNGC 9 cut(s) 204, 758, 1160, 1611, 2030, 2403, 2493, 2766, 2808
FspBI CTAG 7 cut(s) 474, 1445, 2060, 2562, 2592, 2769, 2777
GlaI GCGC 1 cut(s) 1847
GluI GCNGC 9 cut(s) 204, 758, 1160, 1611, 2030, 2403, 2493, 2766, 2808
GsaI CCCAGC 4 cut(s) 1344, 1789, 2078, 2305
GsuI CTGGAG 3 cut(s) 468, 1959, 2871
HaeIII GGCC 6 cut(s) 206, 1200, 1248, 1506, 1714, 2032
HapII CCGG 2 cut(s) 1824, 2869
HgaI GACGC 2 cut(s) 33, 1334
HhaI GCGC 1 cut(s) 1848
Hin1I GRCGYC 1 cut(s) 1345
Hin6I GCGC 1 cut(s) 1846
HinP1I GCGC 1 cut(s) 1846
HindIII AAGCTT 3 cut(s) 1049, 1595, 2354
HpaII CCGG 2 cut(s) 1824, 2869
HphI GGTGA 4 cut(s) 456, 1298, 1325, 1961
Hpy166II GTNNAC 7 cut(s) 730, 732, 1025, 1433, 1910, 2339, 2635
Hpy8I GTNNAC 7 cut(s) 730, 732, 1025, 1433, 1910, 2339, 2635
Hpy99I CGWCG 3 cut(s) 74, 93, 1350
HpyAV CCTTC 9 cut(s) 376, 574, 1178, 1211, 1238, 1329, 1496, 1760, 1827
HpyCH4III ACNGT 6 cut(s) 1839, 1883, 1907, 2336, 2662, 2670
HpyCH4IV ACGT 4 cut(s) 37, 765, 1524, 2649
HpyF10VI GCNNNNNNNGC 8 cut(s) 326, 712, 1265, 1643, 1932, 2498, 2714, 2813
HpySE526I ACGT 4 cut(s) 37, 765, 1524, 2649
Hsp92I GRCGYC 1 cut(s) 1345
HspAI GCGC 1 cut(s) 1846
Kzo9I GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
LmnI GCTCC 6 cut(s) 1444, 1881, 2071, 2436, 2554, 2852
Lsp1109I GCAGC 6 cut(s) 769, 1146, 2414, 2504, 2752, 2794
LweI GCATC 5 cut(s) 938, 1069, 1612, 1634, 2359
MaeI CTAG 7 cut(s) 474, 1445, 2060, 2562, 2592, 2769, 2777
MaeII ACGT 4 cut(s) 37, 765, 1524, 2649
MaeIII GTNAC 6 cut(s) 134, 454, 881, 2360, 2437, 2722
MalI GATC 8 cut(s) 53, 500, 585, 1309, 2139, 2175, 2262, 2478
MbiI CCGCTC 1 cut(s) 1363
MboI GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
MfeI CAATTG 3 cut(s) 330, 1973, 2543
MflI RGATCY 4 cut(s) 498, 1307, 2137, 2173
MhlI GDGCHC 2 cut(s) 414, 2433
MlyI GAGTC 6 cut(s) 55, 94, 167, 281, 1455, 2306
MmeI TCCRAC 2 cut(s) 38, 415
Mph1103I ATGCAT 2 cut(s) 1627, 1959
MroXI GAANNNNTTC 3 cut(s) 175, 1538, 1722
MslI CAYNNNNRTG 1 cut(s) 2367
MspA1I CMGCKG 2 cut(s) 635, 1517
MspCI CTTAAG 2 cut(s) 2279, 2327
MspI CCGG 2 cut(s) 1824, 2869
MspR9I CCNGG 8 cut(s) 419, 550, 1572, 1743, 1751, 1825, 1890, 1938
MunI CAATTG 3 cut(s) 330, 1973, 2543
Mva1269I GAATGC 3 cut(s) 516, 676, 2624
MvaI CCWGG 7 cut(s) 419, 550, 1572, 1743, 1751, 1890, 1938
MvnI CGCG 4 cut(s) 320, 1846, 1848, 2717
MwoI GCNNNNNNNGC 8 cut(s) 326, 712, 1265, 1643, 1932, 2498, 2714, 2813
NciI CCSGG 1 cut(s) 1825
NcoI CCATGG 1 cut(s) 336
NdeII GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
NlaIV GGNNCC 2 cut(s) 172, 526
NmeAIII GCCGAG 1 cut(s) 2398
NmuCI GTSAC 3 cut(s) 134, 881, 2360
NsiI ATGCAT 2 cut(s) 1627, 1959
NspI RCATGY 4 cut(s) 111, 258, 1034, 1961
NspV TTCGAA 1 cut(s) 179
PaeI GCATGC 1 cut(s) 258
PagI TCATGA 1 cut(s) 2263
PaqCI CACCTGC 1 cut(s) 28
PceI AGGCCT 2 cut(s) 1248, 1714
PciI ACATGT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PctI GAATGC 3 cut(s) 516, 676, 2624
PdmI GAANNNNTTC 3 cut(s) 175, 1538, 1722
PfeI GAWTC 7 cut(s) 8, 74, 181, 2085, 2110, 2224, 2654
PflMI CCANNNNNTGG 1 cut(s) 779
PkrI GCNGC 9 cut(s) 205, 759, 1161, 1612, 2031, 2404, 2494, 2767, 2809
PleI GAGTC 6 cut(s) 55, 94, 166, 280, 1455, 2305
PmaCI CACGTG 1 cut(s) 1525
PmlI CACGTG 1 cut(s) 1525
PpsI GAGTC 6 cut(s) 55, 94, 166, 280, 1455, 2305
Ppu21I YACGTR 2 cut(s) 1525, 2650
PscI ACATGT 1 cut(s) 107
PshAI GACNNNNGTC 1 cut(s) 314
PsiI TTATAA 2 cut(s) 192, 995
Psp124BI GAGCTC 2 cut(s) 414, 2433
Psp6I CCWGG 7 cut(s) 417, 548, 1570, 1741, 1749, 1888, 1936
PspCI CACGTG 1 cut(s) 1525
PspFI CCCAGC 4 cut(s) 1340, 1785, 2074, 2301
PspGI CCWGG 7 cut(s) 417, 548, 1570, 1741, 1749, 1888, 1936
PspN4I GGNNCC 2 cut(s) 172, 526
PstI CTGCAG 2 cut(s) 316, 759
PstNI CAGNNNCTG 1 cut(s) 2074
PsuI RGATCY 4 cut(s) 498, 1307, 2137, 2173
PvuII CAGCTG 1 cut(s) 1517
RsaI GTAC 5 cut(s) 731, 1774, 1971, 2461, 2803
RsaNI GTAC 5 cut(s) 730, 1773, 1970, 2460, 2802
RseI CAYNNNNRTG 1 cut(s) 2367
SacI GAGCTC 2 cut(s) 414, 2433
SatI GCNGC 9 cut(s) 204, 758, 1160, 1611, 2030, 2403, 2493, 2766, 2808
Sau3AI GATC 8 cut(s) 51, 498, 583, 1307, 2137, 2173, 2260, 2476
SchI GAGTC 6 cut(s) 55, 94, 167, 281, 1455, 2306
ScrFI CCNGG 8 cut(s) 419, 550, 1572, 1743, 1751, 1825, 1890, 1938
SduI GDGCHC 2 cut(s) 414, 2433
SfaNI GCATC 5 cut(s) 938, 1069, 1612, 1634, 2359
SfcI CTRYAG 3 cut(s) 312, 755, 2903
SfuI TTCGAA 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 2367
SmlI CTYRAG 5 cut(s) 1598, 1682, 2214, 2279, 2327
SmoI CTYRAG 5 cut(s) 1598, 1682, 2214, 2279, 2327
SphI GCATGC 1 cut(s) 258
SseBI AGGCCT 2 cut(s) 1248, 1714
SspMI CTAG 7 cut(s) 474, 1445, 2060, 2562, 2592, 2769, 2777
SstI GAGCTC 2 cut(s) 414, 2433
StuI AGGCCT 2 cut(s) 1248, 1714
StyD4I CCNGG 8 cut(s) 417, 548, 1570, 1741, 1749, 1823, 1888, 1936
StyI CCWWGG 2 cut(s) 150, 336
TaaI ACNGT 6 cut(s) 1839, 1883, 1907, 2336, 2662, 2670
TaiI ACGT 4 cut(s) 40, 768, 1527, 2652
TaqI TCGA 9 cut(s) 103, 146, 179, 561, 668, 742, 804, 1297, 2083
TaqII GACCGA 1 cut(s) 2750
TatI WGTACW 2 cut(s) 729, 1969
TauI GCSGC 3 cut(s) 206, 1613, 2032
TfiI GAWTC 7 cut(s) 8, 74, 181, 2085, 2110, 2224, 2654
TscAI CASTG 7 cut(s) 139, 780, 1842, 1886, 2208, 2667, 2854
TseFI GTSAC 3 cut(s) 134, 881, 2360
TseI GCWGC 6 cut(s) 757, 1159, 2402, 2492, 2765, 2807
Tsp45I GTSAC 3 cut(s) 134, 881, 2360
TspGWI ACGGA 3 cut(s) 280, 991, 2567
TspRI CASTG 7 cut(s) 139, 780, 1842, 1886, 2208, 2667, 2854
Van91I CCANNNNNTGG 1 cut(s) 779
Vha464I CTTAAG 2 cut(s) 2279, 2327
XagI CCTNNNNNAGG 1 cut(s) 1575
XceI RCATGY 4 cut(s) 111, 258, 1034, 1961
XcmI CCANNNNNNNNNTGG 1 cut(s) 1886
XmnI GAANNNNTTC 3 cut(s) 175, 1538, 1722
XspI CTAG 7 cut(s) 474, 1445, 2060, 2562, 2592, 2769, 2777
Zsp2I ATGCAT 2 cut(s) 1627, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.