MD13G1234200.v1.1

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005703
Physical Location & Seq
Reverse (-)
23409042 .. 23420657
11616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1234200.v1.1.491

Sequence Viewer

Length: 2976 bp
ATGGTTTTAGGGATGGAAGAATCTCCAACGCATGCGAAGCGAAAAGAACCTGAAGCGTCAGAGATCACAGAAACTCCCACCGATGAATTTCCCCTGAAGCGACGCCATTTGACTCGAACATGTGTGCATGAGGTTGCAGTTCCCAGTGACTACGCTTCGACCAAGGATGATTCGGTTTATGGAACCCTTTCGAATCCTGTGTATAATGGCGAGGCGGCCAAGACATACCAATTTACCCTTGATCCCTTTCAGCAAATTTCTGTGGCATGCTTAGAACGGAAGGAGTCAGTTTTGGTCTCCGCCCATACTTCAGCTGGTAAGACTGCAGTCGCAGAGTATGCGATTGCCATGGCTTTTAGAGACAAGCAGAGGGTTATATATACTTCGCCATTGAAGGCTTTGAGCAACCAGAAGTATAGAGAGCTCAGCCAGGAGTTTGATGATGTTGGATTGATGACTGGAGATGTTACACTCTCACCTAATGCTAGTTGTTTGGTTATGACTACTGAGATCCTTAGGGGAATGCTTTACAGGGGTTCAGAGGTTTTAAAGGAAGTCGCCTGGGTTATCTTCGATGAAATTCATTACATGAAGGATCGTGAAAGAGGGGTTGTTTGGGAAGAGAGTATTATATTTATGCCTCCAGCTGTTAAAATGGTTTTTCTTTCAGCCACAATGTCGAACGCGACTGAGTTTGCAGAGTGGATTTGTAATTTGCACAAACAACCTTGTCATGTGGTTTACACAGATTTTCGACCAACTCCTCTGCAGCATTACATTTTTCCAGTGGGTGGGAACGGGTTGTATCTTGTGGTGGATGAGAATGAACAATTCAGGGAGGAAAATTTTGTGAAGCTTCATGATACTTTCTCAAAGCAGAAGTTGGGTGATGGTCACAGAAATAACAAGGCTAGTGGGAGACTTGCAAAAGGTGGGAATGCATCTGGGGGTTCAGACATATTCAAAATTGTGAAGATGATTATGGAACGGAAGTTTCAGCCAGTTATAATTTTCAGCTTCAGTAGAAGAGAGTGTGAACAACATGCGATGTCCATGTCTAAGCTTGATTTTAACAGCCAAGACGAAAAGGATGCTGTTGAACAGGTTTTTCGTAATGCAATACTCTGCTTAAACGAGGAAGACAGGGAGTTGCCTGCTATTGAGTTAATGCTACCACTCCTTCAGCGAGGAATTGCTGTTCATCATTCCGGCCTTCTTCCTGTTATCAAGGAACTGGTAGAACTTCTTTTCCAAGAAGGCCTTGTAAAAGCCCTTTTTGCTACTGAGACATTTGCAATGGGCTTAAATATGCCAGCAAAAACTGTTGTTTTCACAGCTGTTAAGAAGTGGGATGGTGATAGTCACCGGTATATCGGATCTGGTGAGTATATCCAGATGAGTGGAAGGGCTGGGCGTCGTGGCAAAGATGAGCGGGGTATTTGTATCATAATGATTGATGAACAGATGGAAATGAACACACTCAAGGATATGGTTTTGGGTAAACCAGCTCCTTTAGTTAGTACTTTCAGACTCAGCTATTACTCAATTTTGAATTTGTTGAGCCGTGCGGAAGGCCAATTCACAGCTGAACACGTGATAAAGAATTCATTCCACCAATTTCAGTATGAAAAGGCTTTACCTGGTATAGGGGAAAAGGTCTCAAAGCTTGAGGAGGAAGCGGCATTGCTTGATGCATCAGGAGAGGCTGAAGTTGCTGAGTATCATAAGATAAAACTTGATATAGCTCAACTTGAGAAGAAAATGATGTCAGAGATAACAAGACCTGAAAGAGTTCTGTATTTTCTTCTTCCTGGCCGCCTGGTCAAGATTAGGGAAGGTGGTACAGATTGGGGCTGGGGAGTTGTAGTTAATGTGGTTAAAAAGCCATCATCTGGGTTAGGCACACTGTCCTCGCGCGGTGGTGGGTATATAGTGGATACTTTGCTCCACTGTTCTCCTGGTTCAAGTGAGAACAGTTCACAGCCAAAACCTTGTCCTCCTCGTCCTGGAGAAAAGGGTGAAATGCATGTAGTCCCTGTACAATTGCCCCTGATTTCTGCTCTAAGCAAACTCAGAATATCAATCCCTTCTGATTTGCGGCCGTTGGAGGCGAGGCAAAGTATTCTTCTAGCAGTTCAGGAGCTGGGAACTCGTTTTCCACAAGGCCTTCCAAAGCTGAATCCTGTAAAGGATATGGGCATTGAAGATCCTGAAATTGTTGAGTTAGTCAATCAAATAGAGGCTCTTGAGCAGAAATTATACGAACATCCACTGCATAAGTCTCAAGATGTGAACCAGATTAAATGCTTTCAAAGGAAAGCAGAGGTGGATCATGAAATTCAACAACTTAAGTCCAAAATGCGTGAGTCCCAGCTTCAAAAATTTCGTGATGAACTTAAGAACCGTTCACGGGTCTTGAAGAAGCTTGGTCATATCGATGCCGAGGGTGTTGTCCAATTAAAGGGGCGGGCAGCCTGCTTGATAGACACAGGAGATGAACTCCTTGTTACTGAACTGATGTTTAATGGTACATTCAACGATTTGGATCATCATCAAATTGCAGCAATTGCAAGTTGTTTTATTCCAGTAGATAAATCAAATGAGCAGATACAATTGAGGACGGAGCTTGCTAGACCATTACAACAACTCCAAGCGAGTGCAAGAAGAATAGCAGAGATACAACATGAATGCAAATTAGATGTAAACATTGAAGAGTACGTTGAATCAACAGTGAGACCATTCTTGATGGATGTGATCTATTGTTGGTCAAAGGGTGCAAGTTTCGCGGAAGTTACACAAATGACAGATATATTTGAGGGTAGTATCATTAGAGCTGCTAGAAGACTTGATGAATTTTTGAACCAGTTGCGTGCCGCTGCTCATGCTGTGGGGGAAGTTACTTTGGAAGAGAAATTCACTGGAGCAAGTGAAAGCCTCCGGCGAGGTATCATGTTTGCAAATTCCCTGTATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

992

Amino Acids

111.54

Weight (kDa)

6.0

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 82 - 230 4e-26 DEAD/DEAH box helicase
DEXQc_Suv3 PF22527 97 - 214 2.8e-08 DEXQ-box helicase domain of Suv3
Helicase_C PF00271 387 - 474 2.3e-06 Helicase conserved C-terminal domain
MTR4-like_stalk PF21408 530 - 593 6.5e-16 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 595 - 789 2.6e-58 Mtr4-like, beta-barrel domain
DSHCT PF08148 816 - 986 5.6e-55 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1007
AccB7I CCANNNNNTGG 2 cut(s) 791, 1892
AccBSI CCGCTC 1 cut(s) 1432
AccII CGCG 4 cut(s) 686, 1915, 1917, 2786
AclWI GGATC 7 cut(s) 236, 505, 603, 1384, 2201, 2337, 2553
AcoI YGGCCR 3 cut(s) 216, 1813, 2099
AcuI CTGAAG 6 cut(s) 72, 116, 294, 1003, 1166, 1728
AcvI CACGTG 1 cut(s) 1594
AcyI GRCGYC 2 cut(s) 103, 1414
AfaI GTAC 5 cut(s) 1522, 1843, 2040, 2530, 2717
AfiI CCNNNNNNNGG 6 cut(s) 791, 1646, 1892, 2006, 2410, 2461
AflII CTTAAG 2 cut(s) 2348, 2396
AflIII ACRYGT 2 cut(s) 119, 1591
AgeI ACCGGT 1 cut(s) 1365
AjnI CCWGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
AloI GAACNNNNNNTCC 6 cut(s) 1182, 1214, 1233, 1265, 2140, 2172
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 7 cut(s) 301, 354, 913, 1280, 1663, 2286, 2728
AlwI GGATC 7 cut(s) 236, 505, 603, 1384, 2201, 2337, 2553
AlwNI CAGNNNCTG 1 cut(s) 2143
AoxI GGCC 7 cut(s) 216, 1210, 1258, 1573, 1813, 2099, 2164
ApeKI GCWGC 5 cut(s) 769, 2471, 2561, 2834, 2876
AsiGI ACCGGT 1 cut(s) 1365
Asp700I GAANNNNTTC 3 cut(s) 187, 1607, 1791
AspLEI GCGC 1 cut(s) 1917
AsuHPI GGTGA 6 cut(s) 468, 899, 1355, 1367, 1394, 2030
AsuII TTCGAA 1 cut(s) 191
AxyI CCTNAGG 1 cut(s) 515
BanII GRGCYC 1 cut(s) 426
BbrPI CACGTG 1 cut(s) 1594
BbsI GAAGAC 2 cut(s) 1146, 2848
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 5 cut(s) 781, 2483, 2573, 2821, 2863
BccI CCATC 6 cut(s) 7, 884, 1346, 1459, 1894, 2740
BceAI ACGGC 2 cut(s) 1548, 2086
BcgI CGANNNNNNTGC 2 cut(s) 1073, 1107
BciT130I CCWGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
BciVI GTATCC 1 cut(s) 1930
BcoDI GTCTC 7 cut(s) 301, 354, 913, 1280, 1663, 2286, 2728
BfaI CTAG 5 cut(s) 486, 912, 2129, 2631, 2838
BfmI CTRYAG 2 cut(s) 324, 767
BfrI CTTAAG 2 cut(s) 2348, 2396
BfuI GTATCC 1 cut(s) 1930
BlpI GCTNAGC 1 cut(s) 425
BmcAI AGTACT 1 cut(s) 1522
Bme1390I CCNGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 5 cut(s) 950, 1081, 1681, 1703, 2428
BmuI ACTGGG 1 cut(s) 138
BoxI GACNNNNGTC 1 cut(s) 326
BpiI GAAGAC 2 cut(s) 1146, 2848
BplI GAGNNNNNCTC 2 cut(s) 2484, 2516
BpmI CTGGAG 4 cut(s) 480, 627, 2028, 2940
Bpu1102I GCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 191
BpuEI CTTGAG 5 cut(s) 1466, 1688, 1772, 2267, 2268
Bsa29I ATCGAT 1 cut(s) 2436
BsaAI YACGTR 1 cut(s) 1594
BsaBI GATNNNNATC 2 cut(s) 2544, 2550
BsaHI GRCGYC 2 cut(s) 103, 1414
BsaI GGTCTC 3 cut(s) 301, 1663, 2728
BsaJI CCNNGG 4 cut(s) 162, 348, 561, 2442
BsaWI WCCGGW 1 cut(s) 1365
BsaXI ACNNNNNCTCC 6 cut(s) 58, 88, 1851, 1881, 2631, 2661
Bsc4I CCNNNNNNNGG 6 cut(s) 791, 1646, 1892, 2006, 2410, 2461
Bse118I RCCGGY 1 cut(s) 1365
Bse1I ACTGG 8 cut(s) 144, 463, 785, 1001, 1239, 2585, 2863, 2923
Bse21I CCTNAGG 1 cut(s) 515
Bse3DI GCAATG 2 cut(s) 1302, 1682
Bse8I GATNNNNATC 2 cut(s) 2544, 2550
BseBI CCWGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
BseCI ATCGAT 1 cut(s) 2436
BseDI CCNNGG 4 cut(s) 162, 348, 561, 2442
BseGI GGATG 7 cut(s) 18, 172, 823, 1096, 1357, 2266, 2755
BseJI GATNNNNATC 2 cut(s) 2544, 2550
BseLI CCNNNNNNNGG 6 cut(s) 791, 1646, 1892, 2006, 2410, 2461
BseMI GCAATG 2 cut(s) 1302, 1682
BseMII CTCAG 7 cut(s) 439, 498, 681, 1275, 1546, 1707, 2086
BseNI ACTGG 8 cut(s) 144, 463, 785, 1001, 1239, 2585, 2863, 2923
BseRI GAGGAG 3 cut(s) 753, 1685, 1989
BseX3I CGGCCG 1 cut(s) 2099
BseXI GCAGC 5 cut(s) 781, 2483, 2573, 2821, 2863
BseYI CCCAGC 4 cut(s) 1409, 1854, 2143, 2370
Bsh1236I CGCG 4 cut(s) 686, 1915, 1917, 2786
Bsh1285I CGRYCG 1 cut(s) 2102
BshFI GGCC 7 cut(s) 218, 1212, 1260, 1575, 1815, 2101, 2166
BshTI ACCGGT 1 cut(s) 1365
BshVI ATCGAT 1 cut(s) 2436
BsiEI CGRYCG 1 cut(s) 2102
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 3 cut(s) 1209, 1366, 2938
BslFI GGGAC 2 cut(s) 2018, 2353
BslI CCNNNNNNNGG 6 cut(s) 791, 1646, 1892, 2006, 2410, 2461
BsmAI GTCTC 7 cut(s) 301, 354, 913, 1280, 1663, 2286, 2728
BsmFI GGGAC 2 cut(s) 2018, 2353
BsmI GAATGC 3 cut(s) 528, 943, 2693
BsnI GGCC 7 cut(s) 218, 1212, 1260, 1575, 1815, 2101, 2166
Bso31I GGTCTC 3 cut(s) 301, 1663, 2728
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 426
Bsp1407I TGTACA 1 cut(s) 2038
Bsp143I GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
Bsp1720I GCTNAGC 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 348
BspANI GGCC 7 cut(s) 218, 1212, 1260, 1575, 1815, 2101, 2166
BspCNI CTCAG 7 cut(s) 438, 499, 682, 1276, 1545, 1708, 2085
BspDI ATCGAT 1 cut(s) 2436
BspFNI CGCG 4 cut(s) 686, 1915, 1917, 2786
BspHI TCATGA 2 cut(s) 859, 2332
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 2 cut(s) 328, 771
BspPI GGATC 7 cut(s) 236, 505, 603, 1384, 2201, 2337, 2553
BspT104I TTCGAA 1 cut(s) 191
BspTI CTTAAG 2 cut(s) 2348, 2396
BspTNI GGTCTC 3 cut(s) 301, 1663, 2728
BsrBI CCGCTC 1 cut(s) 1432
BsrDI GCAATG 2 cut(s) 1302, 1682
BsrFI RCCGGY 1 cut(s) 1365
BsrGI TGTACA 1 cut(s) 2038
BsrI ACTGG 8 cut(s) 144, 463, 785, 1001, 1239, 2585, 2863, 2923
BssAI RCCGGY 1 cut(s) 1365
BssECI CCNNGG 4 cut(s) 162, 348, 561, 2442
BssMI GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
BssNI GRCGYC 2 cut(s) 103, 1414
BssT1I CCWWGG 2 cut(s) 162, 348
Bst2UI CCWGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
Bst4CI ACNGT 6 cut(s) 1324, 1908, 1952, 1976, 2405, 2731
Bst6I CTCTTC 4 cut(s) 615, 1021, 2706, 2901
BstACI GRCGYC 2 cut(s) 103, 1414
BstAFI CTTAAG 2 cut(s) 2348, 2396
BstAPI GCANNNNNTGC 2 cut(s) 338, 2567
BstAUI TGTACA 1 cut(s) 2038
BstBAI YACGTR 1 cut(s) 1594
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 8 cut(s) 33, 268, 1155, 1314, 2469, 2476, 2628, 2871
BstDSI CCRYGG 1 cut(s) 348
BstENI CCTNNNNNAGG 1 cut(s) 1644
BstF5I GGATG 7 cut(s) 18, 172, 823, 1096, 1357, 2266, 2755
BstFNI CGCG 4 cut(s) 686, 1915, 1917, 2786
BstHHI GCGC 1 cut(s) 1917
BstKTI GATC 9 cut(s) 66, 244, 513, 598, 1379, 2209, 2332, 2548, 2757
BstMAI GTCTC 7 cut(s) 301, 354, 913, 1280, 1663, 2286, 2728
BstMBI GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
BstMCI CGRYCG 1 cut(s) 2102
BstMWI GCNNNNNNNGC 7 cut(s) 37, 338, 1277, 1712, 2567, 2783, 2882
BstNI CCWGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
BstNSI RCATGY 5 cut(s) 35, 123, 270, 1046, 2030
BstPAI GACNNNNGTC 1 cut(s) 326
BstSCI CCNGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
BstSFI CTRYAG 2 cut(s) 324, 767
BstUI CGCG 4 cut(s) 686, 1915, 1917, 2786
BstV1I GCAGC 5 cut(s) 781, 2483, 2573, 2821, 2863
BstV2I GAAGAC 2 cut(s) 1146, 2848
BstX2I RGATCY 3 cut(s) 510, 1376, 2206
BstXI CCANNNNNNTGG 1 cut(s) 1400
BstYI RGATCY 3 cut(s) 510, 1376, 2206
BstZI CGGCCG 1 cut(s) 2099
Bsu15I ATCGAT 1 cut(s) 2436
Bsu36I CCTNAGG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 1930
BsuRI GGCC 7 cut(s) 218, 1212, 1260, 1575, 1815, 2101, 2166
BsuTUI ATCGAT 1 cut(s) 2436
BtgI CCRYGG 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 1061
BtsCI GGATG 7 cut(s) 18, 172, 823, 1096, 1357, 2266, 2755
BtsI GCAGTG 1 cut(s) 2270
BtsIMutI CAGTG 7 cut(s) 151, 792, 1904, 1948, 2270, 2736, 2916
Cac8I GCNNGC 8 cut(s) 33, 268, 1155, 1314, 2469, 2476, 2628, 2871
CaiI CAGNNNCTG 1 cut(s) 2143
CciI TCATGA 2 cut(s) 859, 2332
CfoI GCGC 1 cut(s) 1917
Cfr10I RCCGGY 1 cut(s) 1365
ClaI ATCGAT 1 cut(s) 2436
CseI GACGC 3 cut(s) 45, 111, 1403
CsiI ACCWGGT 1 cut(s) 1639
Csp6I GTAC 5 cut(s) 1521, 1842, 2039, 2529, 2716
CspAI ACCGGT 1 cut(s) 1365
CspCI CAANNNNNGTGG 2 cut(s) 895, 930
CviQI GTAC 5 cut(s) 1521, 1842, 2039, 2529, 2716
DpnI GATC 9 cut(s) 65, 243, 512, 597, 1378, 2208, 2331, 2547, 2756
DpnII GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
DraI TTTAAA 1 cut(s) 549
EaeI YGGCCR 3 cut(s) 216, 1813, 2099
EagI CGGCCG 1 cut(s) 2099
Eam1104I CTCTTC 4 cut(s) 615, 1021, 2706, 2901
EarI CTCTTC 4 cut(s) 615, 1021, 2706, 2901
EciI GGCGGA 1 cut(s) 289
Ecl136II GAGCTC 1 cut(s) 424
EclXI CGGCCG 1 cut(s) 2099
Eco130I CCWWGG 2 cut(s) 162, 348
Eco147I AGGCCT 2 cut(s) 1260, 2166
Eco24I GRGCYC 1 cut(s) 426
Eco31I GGTCTC 3 cut(s) 301, 1663, 2728
Eco52I CGGCCG 1 cut(s) 2099
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 6 cut(s) 72, 116, 294, 1003, 1166, 1728
Eco72I CACGTG 1 cut(s) 1594
Eco81I CCTNAGG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 1644
EcoRI GAATTC 1 cut(s) 1603
EcoRII CCWGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
EcoT14I CCWWGG 2 cut(s) 162, 348
EcoT22I ATGCAT 3 cut(s) 943, 1696, 2028
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 2 cut(s) 162, 348
FalI AAGNNNNNCTT 2 cut(s) 1245, 1277
FaqI GGGAC 2 cut(s) 2018, 2353
FauI CCCGC 2 cut(s) 1425, 2460
FokI GGATG 7 cut(s) 25, 179, 830, 1103, 1364, 2253, 2762
FriOI GRGCYC 1 cut(s) 426
FspBI CTAG 5 cut(s) 486, 912, 2129, 2631, 2838
GlaI GCGC 1 cut(s) 1916
GsaI CCCAGC 4 cut(s) 1413, 1858, 2147, 2374
GsuI CTGGAG 4 cut(s) 480, 627, 2028, 2940
HaeIII GGCC 7 cut(s) 218, 1212, 1260, 1575, 1815, 2101, 2166
HapII CCGG 3 cut(s) 1209, 1366, 2938
HgaI GACGC 3 cut(s) 45, 111, 1403
HhaI GCGC 1 cut(s) 1917
Hin1I GRCGYC 2 cut(s) 103, 1414
Hin6I GCGC 1 cut(s) 1915
HinP1I GCGC 1 cut(s) 1915
HindIII AAGCTT 4 cut(s) 854, 1061, 1664, 2423
HinfI GANTC 9 cut(s) 20, 112, 170, 193, 284, 1530, 2179, 2366, 2723
HpaII CCGG 3 cut(s) 1209, 1366, 2938
HphI GGTGA 6 cut(s) 468, 899, 1355, 1367, 1394, 2030
Hpy166II GTNNAC 7 cut(s) 742, 1037, 1502, 1979, 2293, 2408, 2704
Hpy188I TCNGA 8 cut(s) 61, 541, 955, 1376, 1529, 1771, 2075, 2092
Hpy8I GTNNAC 7 cut(s) 742, 1037, 1502, 1979, 2293, 2408, 2704
Hpy99I CGWCG 2 cut(s) 105, 1419
HpyCH4III ACNGT 6 cut(s) 1324, 1908, 1952, 1976, 2405, 2731
HpyCH4IV ACGT 2 cut(s) 1593, 2718
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 338, 1277, 1712, 2567, 2783, 2882
HpySE526I ACGT 2 cut(s) 1593, 2718
Hsp92I GRCGYC 2 cut(s) 103, 1414
HspAI GCGC 1 cut(s) 1915
Kzo9I GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
LmnI GCTCC 5 cut(s) 1513, 1950, 2140, 2623, 2921
Lsp1109I GCAGC 5 cut(s) 781, 2483, 2573, 2821, 2863
LweI GCATC 5 cut(s) 950, 1081, 1681, 1703, 2428
MabI ACCWGGT 1 cut(s) 1639
MaeI CTAG 5 cut(s) 486, 912, 2129, 2631, 2838
MaeII ACGT 2 cut(s) 1593, 2718
MaeIII GTNAC 7 cut(s) 146, 466, 893, 1361, 2506, 2791, 2896
MalI GATC 9 cut(s) 65, 243, 512, 597, 1378, 2208, 2331, 2547, 2756
MbiI CCGCTC 1 cut(s) 1432
MboI GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
MfeI CAATTG 3 cut(s) 2042, 2565, 2612
MflI RGATCY 3 cut(s) 510, 1376, 2206
MhlI GDGCHC 1 cut(s) 426
MlyI GAGTC 4 cut(s) 106, 293, 1524, 2375
MmeI TCCRAC 3 cut(s) 50, 427, 2085
Mph1103I ATGCAT 3 cut(s) 943, 1696, 2028
MroXI GAANNNNTTC 3 cut(s) 187, 1607, 1791
MslI CAYNNNNRTG 1 cut(s) 2436
MspA1I CMGCKG 5 cut(s) 314, 647, 1337, 1586, 2876
MspCI CTTAAG 2 cut(s) 2348, 2396
MspI CCGG 3 cut(s) 1209, 1366, 2938
MspR9I CCNGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
MunI CAATTG 3 cut(s) 2042, 2565, 2612
Mva1269I GAATGC 3 cut(s) 528, 943, 2693
MvaI CCWGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
MvnI CGCG 4 cut(s) 686, 1915, 1917, 2786
MwoI GCNNNNNNNGC 7 cut(s) 37, 338, 1277, 1712, 2567, 2783, 2882
NcoI CCATGG 1 cut(s) 348
NdeII GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 1 cut(s) 2467
NmuCI GTSAC 3 cut(s) 146, 893, 1361
NsiI ATGCAT 3 cut(s) 943, 1696, 2028
NspI RCATGY 5 cut(s) 35, 123, 270, 1046, 2030
NspV TTCGAA 1 cut(s) 191
PaeI GCATGC 2 cut(s) 35, 270
PagI TCATGA 2 cut(s) 859, 2332
PceI AGGCCT 2 cut(s) 1260, 2166
PciI ACATGT 1 cut(s) 119
PctI GAATGC 3 cut(s) 528, 943, 2693
PdmI GAANNNNTTC 3 cut(s) 187, 1607, 1791
PfeI GAWTC 5 cut(s) 20, 170, 193, 2179, 2723
PflMI CCANNNNNTGG 2 cut(s) 791, 1892
PfoI TCCNGGA 1 cut(s) 2005
PinAI ACCGGT 1 cut(s) 1365
PleI GAGTC 4 cut(s) 106, 292, 1524, 2374
PmaCI CACGTG 1 cut(s) 1594
PmlI CACGTG 1 cut(s) 1594
PpsI GAGTC 4 cut(s) 106, 292, 1524, 2374
Ppu21I YACGTR 1 cut(s) 1594
PscI ACATGT 1 cut(s) 119
PshAI GACNNNNGTC 1 cut(s) 326
PsiI TTATAA 1 cut(s) 1007
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
PspCI CACGTG 1 cut(s) 1594
PspFI CCCAGC 4 cut(s) 1409, 1854, 2143, 2370
PspGI CCWGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
PspN4I GGNNCC 1 cut(s) 184
PstI CTGCAG 2 cut(s) 328, 771
PstNI CAGNNNCTG 1 cut(s) 2143
PsuI RGATCY 3 cut(s) 510, 1376, 2206
PvuII CAGCTG 4 cut(s) 314, 647, 1337, 1586
RsaI GTAC 5 cut(s) 1522, 1843, 2040, 2530, 2717
RsaNI GTAC 5 cut(s) 1521, 1842, 2039, 2529, 2716
RseI CAYNNNNRTG 1 cut(s) 2436
SacI GAGCTC 1 cut(s) 426
Sau3AI GATC 9 cut(s) 63, 241, 510, 595, 1376, 2206, 2329, 2545, 2754
ScaI AGTACT 1 cut(s) 1522
SchI GAGTC 4 cut(s) 106, 293, 1524, 2375
ScrFI CCNGG 7 cut(s) 431, 562, 1641, 1812, 1820, 1959, 2007
SduI GDGCHC 1 cut(s) 426
SexAI ACCWGGT 1 cut(s) 1639
SfaNI GCATC 5 cut(s) 950, 1081, 1681, 1703, 2428
SfcI CTRYAG 2 cut(s) 324, 767
SfuI TTCGAA 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 2436
SmlI CTYRAG 7 cut(s) 1481, 1667, 1751, 2246, 2283, 2348, 2396
SmoI CTYRAG 7 cut(s) 1481, 1667, 1751, 2246, 2283, 2348, 2396
SphI GCATGC 2 cut(s) 35, 270
SseBI AGGCCT 2 cut(s) 1260, 2166
SspMI CTAG 5 cut(s) 486, 912, 2129, 2631, 2838
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 2 cut(s) 1260, 2166
StyD4I CCNGG 7 cut(s) 429, 560, 1639, 1810, 1818, 1957, 2005
StyI CCWWGG 2 cut(s) 162, 348
TaaI ACNGT 6 cut(s) 1324, 1908, 1952, 1976, 2405, 2731
TaiI ACGT 2 cut(s) 1596, 2721
TaqI TCGA 7 cut(s) 115, 158, 191, 573, 680, 754, 2436
TatI WGTACW 2 cut(s) 1520, 2038
TauI GCSGC 5 cut(s) 218, 1682, 1818, 2101, 2876
TfiI GAWTC 5 cut(s) 20, 170, 193, 2179, 2723
TscAI CASTG 7 cut(s) 151, 792, 1911, 1955, 2277, 2736, 2923
TseFI GTSAC 3 cut(s) 146, 893, 1361
TseI GCWGC 5 cut(s) 769, 2471, 2561, 2834, 2876
Tsp45I GTSAC 3 cut(s) 146, 893, 1361
TspGWI ACGGA 3 cut(s) 292, 1003, 2636
TspRI CASTG 7 cut(s) 151, 792, 1911, 1955, 2277, 2736, 2923
Van91I CCANNNNNTGG 2 cut(s) 791, 1892
Vha464I CTTAAG 2 cut(s) 2348, 2396
XagI CCTNNNNNAGG 1 cut(s) 1644
XceI RCATGY 5 cut(s) 35, 123, 270, 1046, 2030
XcmI CCANNNNNNNNNTGG 2 cut(s) 311, 1955
XmnI GAANNNNTTC 3 cut(s) 187, 1607, 1791
XspI CTAG 5 cut(s) 486, 912, 2129, 2631, 2838
ZrmI AGTACT 1 cut(s) 1522
Zsp2I ATGCAT 3 cut(s) 943, 1696, 2028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.