Rh4AG080500

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005703
Physical Location & Seq
Reverse (-)
16729000 .. 16736986
7987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG080500.1

Sequence Viewer

Length: 2829 bp
ATGGAAGAATCTCAAACACTTGGAAAACGAAAAGAGCCTGAAGGGTCTGAGAATTTAGAAAACCCTAATGAAGGGTCTCCCCTGAAGCGAAGAAACTTCACTAGAACATGTATGCATGAGGTTGCAGTCCCTAGTGGGTATGCTCCCACCAAGGATGAGTCGGTTCATGGAACCCTCTCGAATCCTGTGTTTGAAGGGAAGATGGCTAAGACATATAAGTTTGAGCTTGATCCGTTTCAGCAGATATCTGTGGCGTGTTTAGAGAGGAATGAGTCGGTTTTGGTGTCGGCGCATACTTCGGCGGGGAAGACTGCGGTGGCGGAGTATGCGATTGCTATGGCGTTTAGGGATAAGCAGAGGGTTATATATACTTCGCCGGTGAAGGCATTGAGCAATCAGAAGTATCGGGAGTTCAATCAGGAGTTTGAAGATGTCGGATTGATGACTGGAGATGTTACGATATCGCCTAATGCGAGTTGTTTGGTCATGACTACTGAGATCCTTAGAGGAATGCTTTACAGGGGTTCGGAGGTTTTGAAGGAAGTTGCGTGGGTTATCTTTGATGAGATTCATTACATGAAGGATCGGGAAAGAGGGGTGGTTTGGGAAGAGAGTATTATATTTATGCCGCCAGCTGTTAAAATGGTGTTTCTTTCAGCCACAATGTCGAATGCCACTGAGTTTGCGGAATGGATTTGTAATGTGCACAAGCAGCCTTGTCATGTGGTGTACACAGATTTTCGACCAACTCCTCTGCAGCATTACATTTTCCCAGTGGGTGGGAATGGCTTGTATCTTGTGGTGGACGAGAATGAACAATTCAAGGAGGACAATTTTATGAAGCTGCAGGATAATTTCTCCAAGCAGAAAGTGGGTGAAGGTCACAGGAGTGCAAATGGCAAGGGAAGTGGGAGAATTGCAAAAGGCGGGAGTGCTGCATCTGGGGGTTCTGACATATTCAAAATTGTGAAGATGATTATGGAAAAGAAATTTCAGCCAGTTATTATTTTCAGCTTCAGTAGAAGAGAATGTGAACATCATGCGTTGAACATGGCTAAGCTTGACTTTAACTCCCAAGAGGAGAAGGATGTTGTTGAACAGGTTTTTCGCAATGCAATACTCTGCTTAAATGAGGAAGACAGAGAGTTGCCTGCTATTGAGTTAATCTTGCCACTCCTTCTGCGAGGCATTGCTGTTCATCATTCTGGCCTTCTTCCTGTTATCAAGGAATTGGTAGAACTTCTTTTTCAGGAGGGCCTTGTAAAGGCCCTCTTTGCTACTGAGACATTTGCAATGGGTATAAACATGCCAGCAAAAACTGTTGTTTTCACAGCTGTGAAAAAGTGGGATGGTGATAGTCATCGATATATTGGGTCTGGGGAGTATATCCAGATGAGCGGTAGGGCCGGGCGTCGTGGCAAAGATGAGCAAGGTATTTGTATCATAATGATTGATGAGCAGATGGAAATGAACACGCTCAAGGACATGGTGTTGGGTAAACCAGCTCCTTTGGTTAGTACTTTCAGACTCAGCTACTACTCAATTTTGAATTTGCTGAGCCGGGCAGAAGGCCAATTCACAGCTGAACACGTGATAAAGAATTCCTTTCACCAATTTCAGCATGAAAAGGCCTTACCTGATATAGGGAAGAAGGTTTCACAGCTTGAGCAGGAAGCGGAAGTGCTTGATTCATCAGGAGAGGCTGAAGTTGCAGAGTATGATAAGATAAAACTTGATATAGCCCAACTTGAGAAGAAAATGATGTCAGAGATAATGAGGCCTGAAAGAGTTCTAATTTTTCTTCTTACTGGTCGCTTGGTCAAGATAAGGGAAGGTGGAACAGATTGGGGTTGGGGAGTTGTAGTTAATGTTGTTAAAAAGCCTTCATCAGGGTTAGGCACAGCTTCTTCACGTGGTGGTGGTTATATAGTGGATACTTTGCTTCACTGTTCTCCTAGTTCAAGTGAGAACAGTTCACAGCCAAAACCATGTCCCCCTCGTCCTGGAGAGAAGGGTGAAATGCATGTAGTCCCTGTTCAGTTGCCCCTGATATCTGCTCTAAGCAAACTGCGAATAAATGTTCCTTCTGATTTGCGGCCACTGGAGGCAAGGCAAAGTATTCTTCTTGCAGTGCAGGAACTGGGAATACGTTTTCCACAAGGCCTTCCAAAGCTGAATCCTGTAAAGGATATGGGCATTGAAGATCCCGAAATTGTTGAGCTGGTTAATCAAATAGAAGCACTTGAACAGAGATTGTATGCACACCCACTGCATAAGTCTCAAGATGTGCACCAGATTAAATGTTTTCAAAGGAAAGCAGAGGTGAATCATGAAATTCAACAACTTAAGTCCAAGATGCGTGAATCACAGCTTCAAAAATTTCGTGATGAACTTAAGAACCGTTCAAGGGTCTTAAAGAAGCTTGGTCATATTAATGCTGAGGGCGTTGTTCAGTTAAAGGGGCGGGCAGCCTGCTTGATAGACACCGGAGATGAACTTCTTGTTACGGAGCTGATGTTTAATGGTACATTCAACGATTTAGATCATCATCAAGTTGCAGCTCTTGCAAGTTGCTTTATTCCAGGAGATAGATCAAATGAGCAGATACAGTTGAGGTCAGAGCTTGCGAGGCCCTTACAACAACTCCAAGACAGTGCAAGAAGAATAGCAGAGATACAAAATGAATGCAAATTAGAAACAGATGTAGATGAGTATGTGGAATCAACAGTGAGGCCATTCTTGATGGATGTGATTTATTGTTGGTCAAAGGTAAGTGTGTTATTTTGTTGTATAGGACAAAAGTGGAGCAAGGAAATGATATCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

942

Amino Acids

106.04

Weight (kDa)

6.15

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 78 - 226 3e-26 DEAD/DEAH box helicase
ResIII PF04851 83 - 222 1.3e-06 Type III restriction enzyme, res subunit
DEXQc_Suv3 PF22527 98 - 210 2.1e-08 DEXQ-box helicase domain of Suv3
Helicase_C PF00271 397 - 473 4.6e-06 Helicase conserved C-terminal domain
MTR4-like_stalk PF21408 529 - 591 4.1e-15 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 594 - 788 2.6e-57 Mtr4-like, beta-barrel domain
DSHCT PF08148 815 - 923 4.9e-28 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 779
AccBSI CCGCTC 1 cut(s) 1398
AclWI GGATC 4 cut(s) 224, 493, 591, 2198
AcoI YGGCCR 1 cut(s) 2096
AcsI RAATTY 6 cut(s) 52, 989, 1549, 1600, 2334, 2378
AcuI CTGAAG 4 cut(s) 60, 104, 1000, 1725
AcvI CACGTG 2 cut(s) 1591, 1913
AcyI GRCGYC 1 cut(s) 1411
AdeI CACNNNGTG 1 cut(s) 1916
AfaI GTAC 3 cut(s) 731, 1519, 2527
AfiI CCNNNNNNNGG 7 cut(s) 71, 779, 1264, 1643, 1889, 2003, 2407
AflII CTTAAG 2 cut(s) 2345, 2393
AflIII ACRYGT 2 cut(s) 107, 1588
AjnI CCWGG 2 cut(s) 2002, 2581
AleI CACNNNNGTG 2 cut(s) 888, 1334
Alw21I GWGCWC 2 cut(s) 708, 2292
Alw26I GTCTC 3 cut(s) 81, 1277, 2283
Alw44I GTGCAC 2 cut(s) 704, 2288
AlwI GGATC 4 cut(s) 224, 493, 591, 2198
AlwNI CAGNNNCTG 1 cut(s) 2140
ApaLI GTGCAC 2 cut(s) 704, 2288
ApeKI GCWGC 6 cut(s) 712, 757, 844, 935, 2468, 2558
ApoI RAATTY 6 cut(s) 52, 989, 1549, 1600, 2334, 2378
AseI ATTAAT 1 cut(s) 2433
Asp700I GAANNNNTTC 1 cut(s) 1788
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 4 cut(s) 1255, 1267, 1404, 2631
AsuC2I CCSGG 2 cut(s) 1408, 1562
AsuHPI GGTGA 6 cut(s) 391, 887, 1364, 1601, 2027, 2335
BaeGI GKGCMC 2 cut(s) 708, 2292
BbrPI CACGTG 2 cut(s) 1591, 1913
BbsI GAAGAC 2 cut(s) 314, 1143
Bbv12I GWGCWC 2 cut(s) 708, 2292
BbvCI CCTCAGC 1 cut(s) 2439
BbvI GCAGC 6 cut(s) 724, 769, 831, 922, 2480, 2570
BccI CCATC 4 cut(s) 196, 1343, 1456, 2737
BciT130I CCWGG 2 cut(s) 2004, 2583
BciVI GTATCC 1 cut(s) 1927
BcnI CCSGG 2 cut(s) 1408, 1562
BcoDI GTCTC 3 cut(s) 81, 1277, 2283
BfaI CTAG 3 cut(s) 102, 132, 1956
BfmI CTRYAG 2 cut(s) 755, 845
BfrI CTTAAG 2 cut(s) 2345, 2393
BfuI GTATCC 1 cut(s) 1927
BisI GCNGC 8 cut(s) 629, 713, 758, 845, 936, 2096, 2469, 2559
BlpI GCTNAGC 2 cut(s) 1056, 1556
BlsI GCNGC 8 cut(s) 630, 714, 759, 846, 937, 2097, 2470, 2560
BmcAI AGTACT 1 cut(s) 1519
Bme1390I CCNGG 4 cut(s) 1408, 1562, 2004, 2583
BmgT120I GGNCC 4 cut(s) 1255, 1267, 1404, 2631
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 4 cut(s) 1408, 1562, 2004, 2583
BmrI ACTGGG 2 cut(s) 767, 2150
BmsI GCATC 2 cut(s) 947, 2346
BmuI ACTGGG 2 cut(s) 767, 2150
BpiI GAAGAC 2 cut(s) 314, 1143
BpmI CTGGAG 3 cut(s) 468, 2025, 2123
Bpu10I CCTNAGC 1 cut(s) 2439
Bpu1102I GCTNAGC 2 cut(s) 1056, 1556
BpuEI CTTGAG 4 cut(s) 1463, 1685, 1769, 2265
BpuMI CCSGG 2 cut(s) 1408, 1562
Bsa29I ATCGAT 1 cut(s) 1363
BsaAI YACGTR 2 cut(s) 1591, 1913
BsaBI GATNNNNATC 3 cut(s) 1359, 2541, 2547
BsaHI GRCGYC 1 cut(s) 1411
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 2486
BsaXI ACNNNNNCTCC 8 cut(s) 1055, 1085, 1848, 1878, 2501, 2531, 2628, 2658
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 779, 1264, 1643, 1889, 2003, 2407
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 6 cut(s) 451, 773, 998, 1813, 2106, 2145
Bse3DI GCAATG 3 cut(s) 1117, 1188, 1299
Bse8I GATNNNNATC 3 cut(s) 1359, 2541, 2547
BseBI CCWGG 2 cut(s) 2004, 2583
BseCI ATCGAT 1 cut(s) 1363
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 4 cut(s) 160, 1093, 1354, 2752
BseJI GATNNNNATC 3 cut(s) 1359, 2541, 2547
BseLI CCNNNNNNNGG 7 cut(s) 71, 779, 1264, 1643, 1889, 2003, 2407
BseMI GCAATG 3 cut(s) 1117, 1188, 1299
BseMII CTCAG 7 cut(s) 39, 486, 669, 1272, 1543, 1547, 2430
BseNI ACTGG 6 cut(s) 451, 773, 998, 1813, 2106, 2145
BseRI GAGGAG 2 cut(s) 741, 1094
BseSI GKGCMC 2 cut(s) 708, 2292
BseXI GCAGC 6 cut(s) 724, 769, 831, 922, 2480, 2570
BsgI GTGCAG 1 cut(s) 2153
BshVI ATCGAT 1 cut(s) 1363
BsiHKAI GWGCWC 2 cut(s) 708, 2292
BsiSI CCGG 4 cut(s) 377, 1407, 1561, 2487
BslFI GGGAC 3 cut(s) 113, 1977, 2015
BslI CCNNNNNNNGG 7 cut(s) 71, 779, 1264, 1643, 1889, 2003, 2407
BsmAI GTCTC 3 cut(s) 81, 1277, 2283
BsmFI GGGAC 3 cut(s) 113, 1977, 2015
BsmI GAATGC 3 cut(s) 516, 676, 2690
Bso31I GGTCTC 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 708, 2292
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
Bsp1720I GCTNAGC 2 cut(s) 1056, 1556
BspCNI CTCAG 7 cut(s) 40, 487, 670, 1273, 1542, 1548, 2431
BspDI ATCGAT 1 cut(s) 1363
BspHI TCATGA 2 cut(s) 486, 2329
BspLI GGNNCC 1 cut(s) 172
BspMAI CTGCAG 2 cut(s) 759, 849
BspPI GGATC 4 cut(s) 224, 493, 591, 2198
BspTI CTTAAG 2 cut(s) 2345, 2393
BspTNI GGTCTC 1 cut(s) 81
BsrBI CCGCTC 1 cut(s) 1398
BsrDI GCAATG 3 cut(s) 1117, 1188, 1299
BsrFI RCCGGY 1 cut(s) 376
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 6 cut(s) 451, 773, 998, 1813, 2106, 2145
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
BssNI GRCGYC 1 cut(s) 1411
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 2004, 2583
Bst4CI ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2610, 2654, 2728
Bst6I CTCTTC 2 cut(s) 603, 1018
BstACI GRCGYC 1 cut(s) 1411
BstAFI CTTAAG 2 cut(s) 2345, 2393
BstAPI GCANNNNNTGC 1 cut(s) 2564
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 1591, 1913
BstC8I GCNNGC 6 cut(s) 633, 1152, 1311, 2466, 2473, 2625
BstENI CCTNNNNNAGG 4 cut(s) 69, 1262, 1641, 1887
BstF5I GGATG 4 cut(s) 160, 1093, 1354, 2752
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 6 cut(s) 232, 501, 586, 2206, 2545, 2594
BstMAI GTCTC 3 cut(s) 81, 1277, 2283
BstMBI GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
BstMWI GCNNNNNNNGC 6 cut(s) 326, 712, 1274, 1709, 2564, 2629
BstNI CCWGG 2 cut(s) 2004, 2583
BstNSI RCATGY 3 cut(s) 111, 1309, 2027
BstSCI CCNGG 4 cut(s) 1406, 1560, 2002, 2581
BstSFI CTRYAG 2 cut(s) 755, 845
BstSLI GKGCMC 2 cut(s) 708, 2292
BstV1I GCAGC 6 cut(s) 724, 769, 831, 922, 2480, 2570
BstV2I GAAGAC 2 cut(s) 314, 1143
BstX2I RGATCY 2 cut(s) 498, 2203
BstYI RGATCY 2 cut(s) 498, 2203
Bsu15I ATCGAT 1 cut(s) 1363
BsuI GTATCC 1 cut(s) 1927
BsuTUI ATCGAT 1 cut(s) 1363
BtsCI GGATG 4 cut(s) 160, 1093, 1354, 2752
BtsI GCAGTG 2 cut(s) 2136, 2267
BtsIMutI CAGTG 8 cut(s) 675, 780, 1945, 2099, 2136, 2267, 2659, 2733
Cac8I GCNNGC 6 cut(s) 633, 1152, 1311, 2466, 2473, 2625
CaiI CAGNNNCTG 1 cut(s) 2140
CciI TCATGA 2 cut(s) 486, 2329
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 4 cut(s) 1255, 1267, 1404, 2631
ClaI ATCGAT 1 cut(s) 1363
CseI GACGC 1 cut(s) 1400
Csp6I GTAC 3 cut(s) 730, 1518, 2526
CspCI CAANNNNNGTGG 4 cut(s) 664, 699, 889, 924
CviQI GTAC 3 cut(s) 730, 1518, 2526
DpnI GATC 6 cut(s) 231, 500, 585, 2205, 2544, 2593
DpnII GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
DraIII CACNNNGTG 1 cut(s) 1916
EaeI YGGCCR 1 cut(s) 2096
Eam1104I CTCTTC 2 cut(s) 603, 1018
EarI CTCTTC 2 cut(s) 603, 1018
EciI GGCGGA 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 3 cut(s) 1631, 1780, 2163
Eco31I GGTCTC 1 cut(s) 81
Eco32I GATATC 4 cut(s) 246, 462, 2052, 2820
Eco57I CTGAAG 4 cut(s) 60, 104, 1000, 1725
Eco72I CACGTG 2 cut(s) 1591, 1913
EcoNI CCTNNNNNAGG 4 cut(s) 69, 1262, 1641, 1887
EcoO109I RGGNCCY 3 cut(s) 1255, 1267, 2631
EcoRI GAATTC 1 cut(s) 1600
EcoRII CCWGG 2 cut(s) 2002, 2581
EcoRV GATATC 4 cut(s) 246, 462, 2052, 2820
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 2 cut(s) 117, 2025
ErhI CCWWGG 1 cut(s) 150
FalI AAGNNNNNCTT 6 cut(s) 1049, 1081, 1256, 1288, 1589, 1621
FaqI GGGAC 3 cut(s) 113, 1977, 2015
FauI CCCGC 3 cut(s) 295, 920, 2457
Fnu4HI GCNGC 8 cut(s) 629, 713, 758, 845, 936, 2096, 2469, 2559
FokI GGATG 4 cut(s) 167, 1100, 1361, 2759
Fsp4HI GCNGC 8 cut(s) 629, 713, 758, 845, 936, 2096, 2469, 2559
FspBI CTAG 3 cut(s) 102, 132, 1956
GlaI GCGC 1 cut(s) 291
GluI GCNGC 8 cut(s) 629, 713, 758, 845, 936, 2096, 2469, 2559
GsuI CTGGAG 3 cut(s) 468, 2025, 2123
HapII CCGG 4 cut(s) 377, 1407, 1561, 2487
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 1411
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HindIII AAGCTT 2 cut(s) 1058, 2420
HpaII CCGG 4 cut(s) 377, 1407, 1561, 2487
HphI GGTGA 6 cut(s) 391, 887, 1364, 1601, 2027, 2335
Hpy166II GTNNAC 8 cut(s) 706, 730, 732, 805, 1034, 1499, 1976, 2290
Hpy188I TCNGA 9 cut(s) 49, 399, 437, 529, 952, 1526, 1768, 2089, 2620
Hpy8I GTNNAC 8 cut(s) 706, 730, 732, 805, 1034, 1499, 1976, 2290
Hpy99I CGWCG 1 cut(s) 1416
HpyCH4III ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2610, 2654, 2728
HpyCH4IV ACGT 3 cut(s) 1590, 1912, 2149
HpyF10VI GCNNNNNNNGC 6 cut(s) 326, 712, 1274, 1709, 2564, 2629
HpySE526I ACGT 3 cut(s) 1590, 1912, 2149
Hsp92I GRCGYC 1 cut(s) 1411
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
LmnI GCTCC 4 cut(s) 148, 1510, 2509, 2805
Lsp1109I GCAGC 6 cut(s) 724, 769, 831, 922, 2480, 2570
LweI GCATC 2 cut(s) 947, 2346
MaeI CTAG 3 cut(s) 102, 132, 1956
MaeII ACGT 3 cut(s) 1590, 1912, 2149
MaeIII GTNAC 3 cut(s) 454, 881, 2503
MalI GATC 6 cut(s) 231, 500, 585, 2205, 2544, 2593
MbiI CCGCTC 1 cut(s) 1398
MboI GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
MflI RGATCY 2 cut(s) 498, 2203
MhlI GDGCHC 2 cut(s) 708, 2292
MlyI GAGTC 3 cut(s) 167, 281, 1521
MmeI TCCRAC 1 cut(s) 415
Mph1103I ATGCAT 2 cut(s) 117, 2025
MroXI GAANNNNTTC 1 cut(s) 1788
MslI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
MspA1I CMGCKG 3 cut(s) 635, 1334, 1583
MspCI CTTAAG 2 cut(s) 2345, 2393
MspI CCGG 4 cut(s) 377, 1407, 1561, 2487
MspR9I CCNGG 4 cut(s) 1408, 1562, 2004, 2583
Mva1269I GAATGC 3 cut(s) 516, 676, 2690
MvaI CCWGG 2 cut(s) 2004, 2583
MwoI GCNNNNNNNGC 6 cut(s) 326, 712, 1274, 1709, 2564, 2629
NciI CCSGG 2 cut(s) 1408, 1562
NdeII GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 881
NsiI ATGCAT 2 cut(s) 117, 2025
NspI RCATGY 3 cut(s) 111, 1309, 2027
OliI CACNNNNGTG 2 cut(s) 888, 1334
PagI TCATGA 2 cut(s) 486, 2329
PceI AGGCCT 3 cut(s) 1631, 1780, 2163
PciI ACATGT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 470
PctI GAATGC 3 cut(s) 516, 676, 2690
PdmI GAANNNNTTC 1 cut(s) 1788
PfeI GAWTC 8 cut(s) 8, 181, 568, 1688, 2176, 2326, 2363, 2720
PflMI CCANNNNNTGG 1 cut(s) 779
PfoI TCCNGGA 2 cut(s) 2002, 2581
PkrI GCNGC 8 cut(s) 630, 714, 759, 846, 937, 2097, 2470, 2560
PleI GAGTC 3 cut(s) 166, 280, 1521
PmaCI CACGTG 2 cut(s) 1591, 1913
PmlI CACGTG 2 cut(s) 1591, 1913
PpsI GAGTC 3 cut(s) 166, 280, 1521
Ppu21I YACGTR 2 cut(s) 1591, 1913
PscI ACATGT 1 cut(s) 107
PshBI ATTAAT 1 cut(s) 2433
Psp6I CCWGG 2 cut(s) 2002, 2581
PspCI CACGTG 2 cut(s) 1591, 1913
PspGI CCWGG 2 cut(s) 2002, 2581
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 4 cut(s) 1255, 1267, 1404, 2631
PsrI GAACNNNNNNTAC 6 cut(s) 395, 427, 2019, 2051, 2130, 2162
PstI CTGCAG 2 cut(s) 759, 849
PstNI CAGNNNCTG 1 cut(s) 2140
PsuI RGATCY 2 cut(s) 498, 2203
PvuII CAGCTG 3 cut(s) 635, 1334, 1583
RsaI GTAC 3 cut(s) 731, 1519, 2527
RsaNI GTAC 3 cut(s) 730, 1518, 2526
RseI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
SatI GCNGC 8 cut(s) 629, 713, 758, 845, 936, 2096, 2469, 2559
Sau3AI GATC 6 cut(s) 229, 498, 583, 2203, 2542, 2591
Sau96I GGNCC 4 cut(s) 1255, 1267, 1404, 2631
ScaI AGTACT 1 cut(s) 1519
SchI GAGTC 3 cut(s) 167, 281, 1521
ScrFI CCNGG 4 cut(s) 1408, 1562, 2004, 2583
SduI GDGCHC 2 cut(s) 708, 2292
SfaNI GCATC 2 cut(s) 947, 2346
SfcI CTRYAG 2 cut(s) 755, 845
SgrAI CRCCGGYG 1 cut(s) 376
SmiMI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
SmlI CTYRAG 6 cut(s) 1478, 1664, 1748, 2280, 2345, 2393
SmoI CTYRAG 6 cut(s) 1478, 1664, 1748, 2280, 2345, 2393
SseBI AGGCCT 3 cut(s) 1631, 1780, 2163
SspMI CTAG 3 cut(s) 102, 132, 1956
StuI AGGCCT 3 cut(s) 1631, 1780, 2163
StyD4I CCNGG 4 cut(s) 1406, 1560, 2002, 2581
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2610, 2654, 2728
TaiI ACGT 3 cut(s) 1593, 1915, 2152
TaqI TCGA 4 cut(s) 179, 668, 742, 1363
TatI WGTACW 2 cut(s) 729, 1517
TauI GCSGC 2 cut(s) 631, 2098
TfiI GAWTC 8 cut(s) 8, 181, 568, 1688, 2176, 2326, 2363, 2720
TscAI CASTG 8 cut(s) 682, 780, 1952, 2106, 2136, 2274, 2659, 2733
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 6 cut(s) 712, 757, 844, 935, 2468, 2558
Tsp45I GTSAC 1 cut(s) 881
TspGWI ACGGA 2 cut(s) 222, 2522
TspRI CASTG 8 cut(s) 682, 780, 1952, 2106, 2136, 2274, 2659, 2733
Van91I CCANNNNNTGG 1 cut(s) 779
Vha464I CTTAAG 2 cut(s) 2345, 2393
VneI GTGCAC 2 cut(s) 704, 2288
VspI ATTAAT 1 cut(s) 2433
XagI CCTNNNNNAGG 4 cut(s) 69, 1262, 1641, 1887
XapI RAATTY 6 cut(s) 52, 989, 1549, 1600, 2334, 2378
XceI RCATGY 3 cut(s) 111, 1309, 2027
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmnI GAANNNNTTC 1 cut(s) 1788
XspI CTAG 3 cut(s) 102, 132, 1956
ZrmI AGTACT 1 cut(s) 1519
Zsp2I ATGCAT 2 cut(s) 117, 2025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.