Rroxscaffold_5G00342560

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005703
Physical Location & Seq
Reverse (-)
11515124 .. 11523223
8100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342560.1

Sequence Viewer

Length: 2988 bp
ATGGAAGAATCTCAAACACTTGGAAAACGAAAAGAGCCTGAAGGGTCTGAGAATTTAGAAAACCCTAATGAAGGGTCTCCCCTGAAGCGAAGAAACTTCACTAGAACATGTATGCATGAGGTTGCAGTCCCTAGTGGGTATGCTCCCACCAAGGATGAGTCGGTTCATGGAACCCTCTCGAATCCTGTGTTTGAAGGGAAGATGGCTAAGACGTATAAGTTTGAGCTTGATCCGTTTCAGCAGATATCTGTGGCGTGTTTAGAGAGGAATGAGTCGGTTTTGGTTTCGGCGCATACTTCGGCGGGGAAGACTGCGGTGGCGGAGTATGCAATTGCCATGGCGTTTAGGGATAAGCAGAGGGTTATATATACTTCGCCGGTGAAGGCTTTGAGCAATCAGAAGTATCGGGAGTTTAACCAGGAGTTTGAAGATGTCGGATTGATGACTGGAGATGTTACAATATCGCCGAATGCGAGTTGTTTGGTCATGACTACTGAGATCCTTAGAGGAATGCTTTACAGGGGTTCGGAGGTTTTGAAGGAAGTTGCGTGGGTTATCTTTGATGAGATTCATTACATGAAGGATCGGGAAAGAGGGGTGGTTTGGGAAGAGAGTATTATATTTATGCCGCCGGCTGTTAAAATGGTTTTTCTTTCGGCCACAATGTCGAATGCCACTGAGTTTGCGGAATGGATTTGTAATGTGCACAAACAGCCTTGTCATGTGGTGTACACAGATTTTCGACCGACTCCTCTGCAGCATTACATTTTCCCAGTGGGCGGGAATGGCTTGTATCTTGTGGTGGACGAGAATGAACAATTCAAGGAGGACAATTTTATGAAGCTGCAGGATAATTTCTCCAAGCAGAAAGTGGGTGATGGTCACAGGAGTGCAAATGGCAAGGGAAGTGGGAGAATTGCAAAAGGCGGGAGTGCTGCATCTGGGGGTTCTGACATATTCAAAATTGTGAAGATGATTATGGAAAAGAAATTTCAGCCAGTTATTATTTTCAGCTTCAGTAGAAGAGAATGTGAACATCATGCATTGAACATGGCTAAGCTTGACTTTAACTCCCAAGAGGAGAAGGATGTTGTTGAACAGGTTTTTCGCAACGCAATACTCTGCTTAAATGAGGAAGACAGAGAGTTGCCTGCTATTGAGTTAATCTTGCCACTCCTTCTGCGAGGCATTGCTGTTCATCATTCTGGCCTTCTTCCTGTTATCAAGGAATTGGTAGAACTCCTTTTTCAGGAGGGCCTTGTAAAGGCCCTCTTTGCTACTGAGACGTTTGCAATGGGTATAAACATGCCAGCAAAAACTGTTGTTTTCACAGCTGTGAAAAAGTGGGATGGTGATAGTCATCGATATATTGGGTCTGGGGAGTATATCCAGATGAGCGGTAGGGCCGGGCGTCGTGGCAAAGATGAGCAAGGTATTTGTATCATAATGATTGATGAGCAGATGGAAATGAACACGCTTAAGGACATGGTGTTGGGTAAACCAGCTCCTTTGGTTAGTACTTTCAGACTCAGCTACTACTCAATTTTGAATTTGCTGAGCCGTGCAGAAGGCCAATTCACAGCTGAACACGTGATAAAGAATTCCTTTCACCAGTTTCAGCATGAAAAGGCCTTACCTGATATAGGGAAGAAGGTTTCACAGCTTGAGCAGGAAGCGGAAGTGCTTGATTCATCAGGAGAGGCTGAAGTTGCAGAGTATGATAAGATAAAACTTGATATAGCCCAACTTGAGAAGAAAATGATGTCAGAGATTATGAGGCCTGAAAGAGTTCTAATTTTTCTTCTTACTGGTCGCTTGGTCAAGATAAGGGAAGGTGGAACAGATTGGGGTTGGGGAGTTGTAGTTAATGTTGTTAAAAAGCCTTCATCAGGGTTAGGCACAGCTTCTTCACGTGGTGGTGGTTATATAGTGGATACTTTGCTTCACTGTTCTCCTAGTTCAAGTGAGAACAGTTCACAGCCAAAACCATGTCCCCCTCGTCCTGGAGAGAAGGGTGAAATGCATGTAGTCCCTGTTCAGTTGCCCCTGATATCTGCTCTAAGCAAACTGCGAATAAATGTTCCTTCTGATTTGCGGCCACTGGAGGCAAGGCAAAGTATTCTTCTTGCAGTGCAGGAACTGGGAATACGTTTTCCACAAGGCCTTCCAAAGCTGAATCCTGTAAAGGATATGGGCATTGAAGATCCCGAAATTGTTGAGCTGGTCAATCAAATAGAAGCACTTGAACAGAGATTGTATGCACACCCACTGCATAAGTCTCAAGATGTGCACCAGATTAAATGTTTTCAAAGGAAAGCAGAGGTGAATCATGAAATTCAACAACTTAAGTCCAAGATGCGTGAATCTCAGCTTCAAAAATTTCGTGATGAACTTAAGAACCGTTCAAGGGTCTTAAAGAAGCTTGGTCATATTAATGCTGAGGGCGTTGTTCAGTTAAAGGGGCGGGCAGCCTGCTTGATAGACACCGGAGATGAACTTCTTGTTACTGAGCTGATGTTTAATGCTAAGCTCAGGAGTGGTACATTCAACGATTTAGATCATCATCAAGTTGCAGCTCTTGCAAGTTGCTTTATTCCAGGAGATAGATCAAATGAGCAGATACAGTTGAGGTCAGAGCTTGCGAGACCCTTACAACAACTCCAAGACAGTGCAAGAAGAATAGCAGAGATACAAAATGAATGCAAATTAGAAACAGATGTAGACGAGTATGTGGAATCAACAGTGAGGCCATTCTTGATGGATGTGATTTATTGTTGGTCAAAGGGTGCAAGTTTTGCAGATGTTATACAAATGACTGAAATATTTGAGGGCAGTATCATTAGATCTGCTAGAAGACTTGATGAATTTTTGAATCAGTTACGCACTGCTGCTAATGCTGTTGGAGAAGTTGATTTAGAGAAGAAATTTGAAGCAGCAAGTGAAAGCCTTCGCCGAGGTATCATGTTTGCAAATTCCCTTTATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

995

Amino Acids

111.88

Weight (kDa)

6.1

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 78 - 226 3.3e-26 DEAD/DEAH box helicase
ResIII PF04851 83 - 222 1.5e-06 Type III restriction enzyme, res subunit
DEXQc_Suv3 PF22527 98 - 210 2.2e-08 DEXQ-box helicase domain of Suv3
Helicase_C PF00271 397 - 473 4.9e-06 Helicase conserved C-terminal domain
MTR4-like_stalk PF21408 529 - 591 4.4e-15 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 594 - 788 2.8e-57 Mtr4-like, beta-barrel domain
DSHCT PF08148 815 - 990 1.1e-51 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1398
AccI GTMKAC 1 cut(s) 2721
AclWI GGATC 4 cut(s) 224, 493, 591, 2198
AcoI YGGCCR 2 cut(s) 657, 2096
AcsI RAATTY 9 cut(s) 52, 989, 1549, 1600, 2334, 2378, 2864, 2924, 2971
AcuI CTGAAG 4 cut(s) 60, 104, 1000, 1725
AcvI CACGTG 2 cut(s) 1591, 1913
AcyI GRCGYC 1 cut(s) 1411
AdeI CACNNNGTG 1 cut(s) 1916
AfaI GTAC 3 cut(s) 731, 1519, 2542
AfiI CCNNNNNNNGG 8 cut(s) 71, 779, 1249, 1264, 1643, 1889, 2003, 2407
AflII CTTAAG 3 cut(s) 1478, 2345, 2393
AflIII ACRYGT 2 cut(s) 107, 1588
AjnI CCWGG 3 cut(s) 417, 2002, 2596
AleI CACNNNNGTG 2 cut(s) 888, 1334
Alw21I GWGCWC 2 cut(s) 708, 2292
Alw26I GTCTC 4 cut(s) 81, 1277, 2283, 2638
Alw44I GTGCAC 2 cut(s) 704, 2288
AlwI GGATC 4 cut(s) 224, 493, 591, 2198
AlwNI CAGNNNCTG 1 cut(s) 2140
ApaLI GTGCAC 2 cut(s) 704, 2288
ApeKI GCWGC 7 cut(s) 757, 844, 935, 2468, 2573, 2888, 2933
ApoI RAATTY 9 cut(s) 52, 989, 1549, 1600, 2334, 2378, 2864, 2924, 2971
AseI ATTAAT 1 cut(s) 2433
Asp700I GAANNNNTTC 2 cut(s) 1788, 2946
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 3 cut(s) 1255, 1267, 1404
AsuC2I CCSGG 1 cut(s) 1408
AsuHPI GGTGA 6 cut(s) 391, 887, 1364, 1601, 2027, 2335
BaeGI GKGCMC 2 cut(s) 708, 2292
BbrPI CACGTG 2 cut(s) 1591, 1913
BbsI GAAGAC 3 cut(s) 314, 1143, 2860
Bbv12I GWGCWC 2 cut(s) 708, 2292
BbvCI CCTCAGC 1 cut(s) 2439
BbvI GCAGC 7 cut(s) 769, 831, 922, 2480, 2585, 2875, 2945
BccI CCATC 5 cut(s) 196, 872, 1343, 1456, 2752
BceAI ACGGC 1 cut(s) 1545
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 3 cut(s) 419, 2004, 2598
BciVI GTATCC 1 cut(s) 1927
BcnI CCSGG 1 cut(s) 1408
BcoDI GTCTC 4 cut(s) 81, 1277, 2283, 2638
BfaI CTAG 4 cut(s) 102, 132, 1956, 2850
BfmI CTRYAG 3 cut(s) 755, 845, 2984
BfrI CTTAAG 3 cut(s) 1478, 2345, 2393
BfuI GTATCC 1 cut(s) 1927
BglII AGATCT 1 cut(s) 2843
BisI GCNGC 9 cut(s) 629, 758, 845, 936, 2096, 2469, 2574, 2889, 2934
BlpI GCTNAGC 3 cut(s) 1056, 1556, 2526
BlsI GCNGC 9 cut(s) 630, 759, 846, 937, 2097, 2470, 2575, 2890, 2935
BmcAI AGTACT 1 cut(s) 1519
Bme1390I CCNGG 4 cut(s) 419, 1408, 2004, 2598
BmgT120I GGNCC 3 cut(s) 1255, 1267, 1404
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 4 cut(s) 419, 1408, 2004, 2598
BmrI ACTGGG 2 cut(s) 767, 2150
BmsI GCATC 2 cut(s) 947, 2346
BmuI ACTGGG 2 cut(s) 767, 2150
BpiI GAAGAC 3 cut(s) 314, 1143, 2860
BpmI CTGGAG 3 cut(s) 468, 2025, 2123
Bpu10I CCTNAGC 2 cut(s) 2439, 2531
Bpu1102I GCTNAGC 3 cut(s) 1056, 1556, 2526
BpuEI CTTGAG 3 cut(s) 1685, 1769, 2265
BpuMI CCSGG 1 cut(s) 1408
Bsa29I ATCGAT 1 cut(s) 1363
BsaAI YACGTR 2 cut(s) 1591, 1913
BsaBI GATNNNNATC 3 cut(s) 1359, 2556, 2562
BsaHI GRCGYC 1 cut(s) 1411
BsaI GGTCTC 2 cut(s) 81, 2638
BsaJI CCNNGG 3 cut(s) 150, 336, 2953
BsaWI WCCGGW 1 cut(s) 2486
BsaXI ACNNNNNCTCC 6 cut(s) 1055, 1085, 1848, 1878, 2643, 2673
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 779, 1249, 1264, 1643, 1889, 2003, 2407
Bse118I RCCGGY 2 cut(s) 376, 631
Bse1I ACTGG 7 cut(s) 451, 773, 998, 1612, 1813, 2106, 2145
Bse3DI GCAATG 2 cut(s) 1188, 1299
Bse8I GATNNNNATC 3 cut(s) 1359, 2556, 2562
BseBI CCWGG 3 cut(s) 419, 2004, 2598
BseCI ATCGAT 1 cut(s) 1363
BseDI CCNNGG 3 cut(s) 150, 336, 2953
BseGI GGATG 4 cut(s) 160, 1093, 1354, 2767
BseJI GATNNNNATC 3 cut(s) 1359, 2556, 2562
BseLI CCNNNNNNNGG 8 cut(s) 71, 779, 1249, 1264, 1643, 1889, 2003, 2407
BseMI GCAATG 2 cut(s) 1188, 1299
BseNI ACTGG 7 cut(s) 451, 773, 998, 1612, 1813, 2106, 2145
BseRI GAGGAG 2 cut(s) 741, 1094
BseSI GKGCMC 2 cut(s) 708, 2292
BseXI GCAGC 7 cut(s) 769, 831, 922, 2480, 2585, 2875, 2945
BsgI GTGCAG 2 cut(s) 1584, 2153
Bsh1285I CGRYCG 1 cut(s) 746
BshVI ATCGAT 1 cut(s) 1363
BsiEI CGRYCG 1 cut(s) 746
BsiHKAI GWGCWC 2 cut(s) 708, 2292
BsiSI CCGG 4 cut(s) 377, 632, 1407, 2487
BslFI GGGAC 3 cut(s) 113, 1977, 2015
BslI CCNNNNNNNGG 8 cut(s) 71, 779, 1249, 1264, 1643, 1889, 2003, 2407
BsmAI GTCTC 4 cut(s) 81, 1277, 2283, 2638
BsmBI CGTCTC 1 cut(s) 1277
BsmFI GGGAC 3 cut(s) 113, 1977, 2015
BsmI GAATGC 4 cut(s) 475, 516, 676, 2705
Bso31I GGTCTC 2 cut(s) 81, 2638
Bsp1286I GDGCHC 2 cut(s) 708, 2292
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
Bsp1720I GCTNAGC 3 cut(s) 1056, 1556, 2526
Bsp19I CCATGG 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 1363
BspHI TCATGA 2 cut(s) 486, 2329
BspLI GGNNCC 1 cut(s) 172
BspMAI CTGCAG 2 cut(s) 759, 849
BspPI GGATC 4 cut(s) 224, 493, 591, 2198
BspTI CTTAAG 3 cut(s) 1478, 2345, 2393
BspTNI GGTCTC 2 cut(s) 81, 2638
BsrBI CCGCTC 1 cut(s) 1398
BsrDI GCAATG 2 cut(s) 1188, 1299
BsrFI RCCGGY 2 cut(s) 376, 631
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 7 cut(s) 451, 773, 998, 1612, 1813, 2106, 2145
BssAI RCCGGY 2 cut(s) 376, 631
BssECI CCNNGG 3 cut(s) 150, 336, 2953
BssMI GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
BssNI GRCGYC 1 cut(s) 1411
BssT1I CCWWGG 2 cut(s) 150, 336
Bst2UI CCWGG 3 cut(s) 419, 2004, 2598
Bst4CI ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2625, 2669, 2743
Bst6I CTCTTC 2 cut(s) 603, 1018
BstACI GRCGYC 1 cut(s) 1411
BstAFI CTTAAG 3 cut(s) 1478, 2345, 2393
BstAPI GCANNNNNTGC 2 cut(s) 2579, 2795
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 1591, 1913
BstC8I GCNNGC 6 cut(s) 633, 1152, 1311, 2466, 2473, 2640
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 5 cut(s) 69, 1247, 1262, 1641, 1887
BstF5I GGATG 4 cut(s) 160, 1093, 1354, 2767
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 7 cut(s) 232, 501, 586, 2206, 2560, 2609, 2846
BstMAI GTCTC 4 cut(s) 81, 1277, 2283, 2638
BstMBI GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
BstMCI CGRYCG 1 cut(s) 746
BstMWI GCNNNNNNNGC 8 cut(s) 326, 712, 786, 1274, 1709, 2579, 2795, 2894
BstNI CCWGG 3 cut(s) 419, 2004, 2598
BstNSI RCATGY 3 cut(s) 111, 1309, 2027
BstSCI CCNGG 4 cut(s) 417, 1406, 2002, 2596
BstSFI CTRYAG 3 cut(s) 755, 845, 2984
BstSLI GKGCMC 2 cut(s) 708, 2292
BstV1I GCAGC 7 cut(s) 769, 831, 922, 2480, 2585, 2875, 2945
BstV2I GAAGAC 3 cut(s) 314, 1143, 2860
BstX2I RGATCY 3 cut(s) 498, 2203, 2843
BstYI RGATCY 3 cut(s) 498, 2203, 2843
Bsu15I ATCGAT 1 cut(s) 1363
BsuI GTATCC 1 cut(s) 1927
BsuTUI ATCGAT 1 cut(s) 1363
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 4 cut(s) 160, 1093, 1354, 2767
BtsI GCAGTG 3 cut(s) 2136, 2267, 2883
BtsIMutI CAGTG 9 cut(s) 675, 780, 1945, 2099, 2136, 2267, 2674, 2748, 2883
Cac8I GCNNGC 6 cut(s) 633, 1152, 1311, 2466, 2473, 2640
CaiI CAGNNNCTG 1 cut(s) 2140
CciI TCATGA 2 cut(s) 486, 2329
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 2 cut(s) 376, 631
Cfr13I GGNCC 3 cut(s) 1255, 1267, 1404
ClaI ATCGAT 1 cut(s) 1363
CseI GACGC 1 cut(s) 1400
Csp6I GTAC 3 cut(s) 730, 1518, 2541
CspCI CAANNNNNGTGG 4 cut(s) 664, 699, 889, 924
CviQI GTAC 3 cut(s) 730, 1518, 2541
DpnI GATC 7 cut(s) 231, 500, 585, 2205, 2559, 2608, 2845
DpnII GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
DraIII CACNNNGTG 1 cut(s) 1916
EaeI YGGCCR 2 cut(s) 657, 2096
Eam1104I CTCTTC 2 cut(s) 603, 1018
EarI CTCTTC 2 cut(s) 603, 1018
EciI GGCGGA 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 150, 336
Eco147I AGGCCT 3 cut(s) 1631, 1780, 2163
Eco31I GGTCTC 2 cut(s) 81, 2638
Eco32I GATATC 2 cut(s) 246, 2052
Eco57I CTGAAG 4 cut(s) 60, 104, 1000, 1725
Eco72I CACGTG 2 cut(s) 1591, 1913
EcoNI CCTNNNNNAGG 5 cut(s) 69, 1247, 1262, 1641, 1887
EcoO109I RGGNCCY 2 cut(s) 1255, 1267
EcoRI GAATTC 1 cut(s) 1600
EcoRII CCWGG 3 cut(s) 417, 2002, 2596
EcoRV GATATC 2 cut(s) 246, 2052
EcoT14I CCWWGG 2 cut(s) 150, 336
EcoT22I ATGCAT 3 cut(s) 117, 1045, 2025
ErhI CCWWGG 2 cut(s) 150, 336
Esp3I CGTCTC 1 cut(s) 1277
FalI AAGNNNNNCTT 6 cut(s) 1049, 1081, 1256, 1288, 1589, 1621
FaqI GGGAC 3 cut(s) 113, 1977, 2015
FauI CCCGC 4 cut(s) 295, 773, 920, 2457
FblI GTMKAC 1 cut(s) 2721
Fnu4HI GCNGC 9 cut(s) 629, 758, 845, 936, 2096, 2469, 2574, 2889, 2934
FokI GGATG 4 cut(s) 167, 1100, 1361, 2774
Fsp4HI GCNGC 9 cut(s) 629, 758, 845, 936, 2096, 2469, 2574, 2889, 2934
FspBI CTAG 4 cut(s) 102, 132, 1956, 2850
GlaI GCGC 1 cut(s) 291
GluI GCNGC 9 cut(s) 629, 758, 845, 936, 2096, 2469, 2574, 2889, 2934
GsuI CTGGAG 3 cut(s) 468, 2025, 2123
HapII CCGG 4 cut(s) 377, 632, 1407, 2487
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 1411
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HindIII AAGCTT 2 cut(s) 1058, 2420
HpaII CCGG 4 cut(s) 377, 632, 1407, 2487
HphI GGTGA 6 cut(s) 391, 887, 1364, 1601, 2027, 2335
Hpy166II GTNNAC 9 cut(s) 706, 730, 732, 805, 1034, 1499, 1976, 2290, 2722
Hpy188I TCNGA 9 cut(s) 49, 399, 437, 529, 952, 1526, 1768, 2089, 2635
Hpy8I GTNNAC 9 cut(s) 706, 730, 732, 805, 1034, 1499, 1976, 2290, 2722
Hpy99I CGWCG 1 cut(s) 1416
HpyCH4III ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2625, 2669, 2743
HpyCH4IV ACGT 5 cut(s) 212, 1286, 1590, 1912, 2149
HpyF10VI GCNNNNNNNGC 8 cut(s) 326, 712, 786, 1274, 1709, 2579, 2795, 2894
HpySE526I ACGT 5 cut(s) 212, 1286, 1590, 1912, 2149
Hsp92I GRCGYC 1 cut(s) 1411
HspAI GCGC 1 cut(s) 290
KroI GCCGGC 1 cut(s) 631
KroNI GCCGGC 1 cut(s) 633
Kzo9I GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
LmnI GCTCC 2 cut(s) 148, 1510
Lsp1109I GCAGC 7 cut(s) 769, 831, 922, 2480, 2585, 2875, 2945
LweI GCATC 2 cut(s) 947, 2346
MaeI CTAG 4 cut(s) 102, 132, 1956, 2850
MaeII ACGT 5 cut(s) 212, 1286, 1590, 1912, 2149
MaeIII GTNAC 4 cut(s) 454, 881, 2503, 2877
MalI GATC 7 cut(s) 231, 500, 585, 2205, 2559, 2608, 2845
MbiI CCGCTC 1 cut(s) 1398
MboI GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 3 cut(s) 498, 2203, 2843
MhlI GDGCHC 2 cut(s) 708, 2292
MlyI GAGTC 4 cut(s) 167, 281, 742, 1521
MmeI TCCRAC 2 cut(s) 415, 2881
Mph1103I ATGCAT 3 cut(s) 117, 1045, 2025
MroNI GCCGGC 1 cut(s) 631
MroXI GAANNNNTTC 2 cut(s) 1788, 2946
MslI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
MspA1I CMGCKG 2 cut(s) 1334, 1583
MspCI CTTAAG 3 cut(s) 1478, 2345, 2393
MspI CCGG 4 cut(s) 377, 632, 1407, 2487
MspR9I CCNGG 4 cut(s) 419, 1408, 2004, 2598
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 4 cut(s) 475, 516, 676, 2705
MvaI CCWGG 3 cut(s) 419, 2004, 2598
MwoI GCNNNNNNNGC 8 cut(s) 326, 712, 786, 1274, 1709, 2579, 2795, 2894
NaeI GCCGGC 1 cut(s) 633
NciI CCSGG 1 cut(s) 1408
NcoI CCATGG 1 cut(s) 336
NdeII GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
NgoMIV GCCGGC 1 cut(s) 631
NlaIV GGNNCC 1 cut(s) 172
NmeAIII GCCGAG 1 cut(s) 2978
NmuCI GTSAC 1 cut(s) 881
NsiI ATGCAT 3 cut(s) 117, 1045, 2025
NspI RCATGY 3 cut(s) 111, 1309, 2027
OliI CACNNNNGTG 2 cut(s) 888, 1334
PagI TCATGA 2 cut(s) 486, 2329
PceI AGGCCT 3 cut(s) 1631, 1780, 2163
PciI ACATGT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 470
PctI GAATGC 4 cut(s) 475, 516, 676, 2705
PdiI GCCGGC 1 cut(s) 633
PdmI GAANNNNTTC 2 cut(s) 1788, 2946
PfeI GAWTC 9 cut(s) 8, 181, 568, 1688, 2176, 2326, 2363, 2735, 2872
PfoI TCCNGGA 2 cut(s) 2002, 2596
PkrI GCNGC 9 cut(s) 630, 759, 846, 937, 2097, 2470, 2575, 2890, 2935
PleI GAGTC 4 cut(s) 166, 280, 742, 1521
PmaCI CACGTG 2 cut(s) 1591, 1913
PmlI CACGTG 2 cut(s) 1591, 1913
PpsI GAGTC 4 cut(s) 166, 280, 742, 1521
Ppu21I YACGTR 2 cut(s) 1591, 1913
PscI ACATGT 1 cut(s) 107
PshBI ATTAAT 1 cut(s) 2433
Psp6I CCWGG 3 cut(s) 417, 2002, 2596
PspCI CACGTG 2 cut(s) 1591, 1913
PspGI CCWGG 3 cut(s) 417, 2002, 2596
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 3 cut(s) 1255, 1267, 1404
PsrI GAACNNNNNNTAC 4 cut(s) 2019, 2051, 2130, 2162
PstI CTGCAG 2 cut(s) 759, 849
PstNI CAGNNNCTG 1 cut(s) 2140
PsuI RGATCY 3 cut(s) 498, 2203, 2843
PvuII CAGCTG 2 cut(s) 1334, 1583
RsaI GTAC 3 cut(s) 731, 1519, 2542
RsaNI GTAC 3 cut(s) 730, 1518, 2541
RseI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
SatI GCNGC 9 cut(s) 629, 758, 845, 936, 2096, 2469, 2574, 2889, 2934
Sau3AI GATC 7 cut(s) 229, 498, 583, 2203, 2557, 2606, 2843
Sau96I GGNCC 3 cut(s) 1255, 1267, 1404
ScaI AGTACT 1 cut(s) 1519
SchI GAGTC 4 cut(s) 167, 281, 742, 1521
ScrFI CCNGG 4 cut(s) 419, 1408, 2004, 2598
SduI GDGCHC 2 cut(s) 708, 2292
SfaNI GCATC 2 cut(s) 947, 2346
SfcI CTRYAG 3 cut(s) 755, 845, 2984
SgrAI CRCCGGYG 1 cut(s) 376
SmiMI CAYNNNNRTG 3 cut(s) 888, 1334, 2433
SmlI CTYRAG 6 cut(s) 1478, 1664, 1748, 2280, 2345, 2393
SmoI CTYRAG 6 cut(s) 1478, 1664, 1748, 2280, 2345, 2393
SseBI AGGCCT 3 cut(s) 1631, 1780, 2163
SspI AATATT 1 cut(s) 2823
SspMI CTAG 4 cut(s) 102, 132, 1956, 2850
StuI AGGCCT 3 cut(s) 1631, 1780, 2163
StyD4I CCNGG 4 cut(s) 417, 1406, 2002, 2596
StyI CCWWGG 2 cut(s) 150, 336
TaaI ACNGT 7 cut(s) 1321, 1949, 1973, 2402, 2625, 2669, 2743
TaiI ACGT 5 cut(s) 215, 1289, 1593, 1915, 2152
TaqI TCGA 4 cut(s) 179, 668, 742, 1363
TaqII GACCGA 1 cut(s) 760
TatI WGTACW 2 cut(s) 729, 1517
TauI GCSGC 2 cut(s) 631, 2098
TfiI GAWTC 9 cut(s) 8, 181, 568, 1688, 2176, 2326, 2363, 2735, 2872
TscAI CASTG 9 cut(s) 682, 780, 1952, 2106, 2136, 2274, 2674, 2748, 2890
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 7 cut(s) 757, 844, 935, 2468, 2573, 2888, 2933
Tsp45I GTSAC 1 cut(s) 881
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 9 cut(s) 682, 780, 1952, 2106, 2136, 2274, 2674, 2748, 2890
Vha464I CTTAAG 3 cut(s) 1478, 2345, 2393
VneI GTGCAC 2 cut(s) 704, 2288
VspI ATTAAT 1 cut(s) 2433
XagI CCTNNNNNAGG 5 cut(s) 69, 1247, 1262, 1641, 1887
XapI RAATTY 9 cut(s) 52, 989, 1549, 1600, 2334, 2378, 2864, 2924, 2971
XceI RCATGY 3 cut(s) 111, 1309, 2027
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 2721
XmnI GAANNNNTTC 2 cut(s) 1788, 2946
XspI CTAG 4 cut(s) 102, 132, 1956, 2850
ZrmI AGTACT 1 cut(s) 1519
Zsp2I ATGCAT 3 cut(s) 117, 1045, 2025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.