FvH4_4g06170

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005703
Physical Location & Seq
Reverse (-)
5532753 .. 5542558
9806 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06170.t2

Sequence Viewer

Length: 2964 bp
ATGGAAGAAGAATCTCAAACCCTTGGAAAACGAAAAGAGCCGGAGCCTTCAGAAACCCCAAACCCTAACGAAGCTTCTCCTCCGAAGCGACAAAACCTCACCAGAACATGTCTCCACGAGGTCGCCGTCCCCGCCGGCTACACTCCGACGAAAGACGAGTCGGTCCACGGTACCCTCTCGAACCCCGCATTCCAAGGCCCCATAGCCAAGACCTACAAGTTCGAGCTCGATCCGTTTCAGAAGATCTCCGTGGCGTGCTTGGAGCGCAACGAGTCGGTTCTCGTCTCGGCGCACACGTCGGCCGGGAAGACTGCGGTTGCGGAGTACGCGATTGCGATGGCGTTTCGAGACAAGCAGAGGGTGATATACACGTCTCCGGTGAAGGCTTTGAGTAATCAGAAGTATAGGGAGTTTAATCAGGAGTTCGAGGATGTTGGATTGATGACTGGGGATGTGACGATTTCGCCGAATGCGAGTTGTTTGGTTATGACTACTGAGATTCTGAGGGGAATGCTTTACAGGGGATCAGAGGTTTTGAAGGAAGTTGCGTGGGTGATTTTCGATGAGATACATTATATGAAGGATAGGGAGAGAGGGGTGGTTTGGGAGGAGAGTATTATATTCATGCCGCCGGAGGTTAAGATGGTGTTTCTCTCGGCTACAATGTCGAATGCCACTGAGTTTGCGGAGTGGATTTGTAATGTGCATAAGCAGCCTTGTCACGTGGTGTACACGGATTTTAGGCCGACTCCGCTGCAGCATTATATTTTCCCCGTGGGTGGGACTGGGTTGCATCTTGTGGTGGACGAGAATGAGCAGTTCAAGGAGGACAATTTTATGAAGCTGCAGGATAGTTTCTCGAAGCAGAAAGTGGGGGAAGGTCACAGGAGTGCGAATGGCAAGGCCGGTGGGAGAATTGCAAAAGGTGGGAGTGCTGGGCCGGGCGGTTCTGACATTTTCAAAATTGTGAAGATGATTATGGAAAAGAAATTTCAGCCCGTTATAATTTTCAGCTTCAGTAGAAGAGAATGTGAACATCATGCAATGGATATGTCTAAGCTTGACTTCAACTCCCAAGAGGAAAAAGATGTTGTTGAACAGGTTTTTCGCAATGCAATACTCTGCTTAAATGAGGAAGACAGAGAGTTGCCTGCTGTTGAGTTAATCTTGCCACTCCTTCTGCGAGGCATTGCTGTGCATCATTCTGGCCTTCTTCCTGTTATCAAGGAATTGGTAGAACTCCTTTTTCAGGAAGGCCTTGTAAAGGCCCTCTTTGCTACTGAGACATTTGCAATGGGTATAAACATGCCAGCAAAAACTGTTGTTTTCACAGCTGTCAAAAAGTGGGATGGTGATAGTCATCGATATATTGGGTCTGGGGAGTATATCCAAATGAGCGGTAGGGCCGGGCGTCGTGGCAAAGATGAGCAAGGTATTTGTATCATAATGATTGATGAGCAGATGGAAATGAACACACTTAAGGACATGGTGTTGGGTAAACCAGCTCCTTTGGTTAGTACTTTCAGACTCAGCTACTATTCAATTTTGAATTTGCTGAGCCGTGCGGAAGGCCAATTCACAGCTGAACATGTGATAAAGAATTCCTTTCACCAATTTCAGCATGAAAAGGCTTTACCTGATATAGGGAAGAAGGTTTCACAGCTTGAGCAGGAAGCAGAAATGCTTGATTCATCAGGAGAGGCTGAAGTTGCAGAGTATGATAAGATAAAACTTGATATAGCCCAACTTGAGAAGAAAATGATGTCAGAGATAATGAGACCTGAAAGAGTTCTAATCTTTCTTCTTACTGGTCGCTTGGTTAAGATAAGGGAAGGTGGAACAGATTGGGGTTGGGGAGTTGTAGTTAATGTTGTTAAAAAGCCTTCATCAGGAGCTTCTTCACGTGGTGGTGGTTATATAGTAGATACTTTGCTTCACTGTTCTCCTGGTTCAAGTGAGAACAGTTCACAGCCAAAACCATGTCCCCCTCGCCCTGGAGAGAAGGGTGAAATGCATGTAGTCCCTGTTCAGTTGCCGCTGATATCTACTCTAAGCAAGCTGCGAATAAATGTTCCTTCTGATTTGCGGCCACTGGAGGCAAGGCAAAATATACTTCTTGCAGTGCAGGAACTGGGAACACGTTTTCCACAAGGCCTTCCAAAGCTGAATCCTGTAAAGGATATGGGCATTCAAGATCCTGAAATTGTTGAGCTGGTTAATCAAATAGAAGCACTTGAAGAGAGATTATATGCACACCCACTGCATAAGTCTCAAGATGTGCACCAGATTAAATGTTTCCAAAGGAAAGCAGAGGTGAATCATGAAATTCAACAACTGAAGTCCAAAATGCGTGAATCACAGCTTCAAAAATTTCGTGATGAACTTAAGAACCGTTCAAGGGTCTTGAAGAAGCTTGGTCATATTAATGCCGAGGGTGTTGTCCAGTTGAAGGGGCGGGCAGCCTGCTTGATAGACACCGGAGATGAACTTCTTGTTACTGAACTGATGTTTAATGGTACATTCAACGATTTAGATCATCACCAAATTGCAGCTCTTGCAAGTTGCTTTATTCCAGGAGATAGATCAAATGAACAGATACAATTGAGGTCAGAGCTTGCAAGACCCTTACAACAACTCCAAGAGAGTGCAAGAAGAATAGCAGAGATACAAAATGAATGCAAATTAGAAACAGATGTAGACGAGTATGTGGAATCAACAGTGAGGCCATTCTTGATGGATGTGATTTATTGTTGGTCAAAGGGTGCAAGTTTTGCGGAAGTTATACAAATGACAGAAATCTTTGAGGGCAGTATCATCAGATCTGCCAGAAGACTTGATGAATTTTTGAACCAGTTGCGAACAGCTGCTAATGCTGTTGGAGAAGTTGATTTGGAGAAGAAATTTGAAGCAGCAAGTGAAAGCCTTCGCCGAGGTATCATGTTTGCAAATTCACTTTATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

988

Amino Acids

111.01

Weight (kDa)

5.94

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 78 - 226 2.1e-26 DEAD/DEAH box helicase
ResIII PF04851 88 - 222 1.7e-06 Type III restriction enzyme, res subunit
DEXQc_Suv3 PF22527 98 - 210 1.9e-08 DEXQ-box helicase domain of Suv3
Helicase_C PF00271 388 - 465 3.8e-06 Helicase conserved C-terminal domain
MTR4-like_stalk PF21408 521 - 583 2.7e-15 Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel PF13234 586 - 777 1.1e-57 Mtr4-like, beta-barrel domain
DSHCT PF08148 804 - 974 4.3e-56 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1004
AasI GACNNNNNNGTC 1 cut(s) 161
Acc65I GGTACC 1 cut(s) 170
AccB1I GGYRCC 1 cut(s) 170
AccBSI CCGCTC 1 cut(s) 1398
AccI GTMKAC 1 cut(s) 2697
AccII CGCG 1 cut(s) 329
AclWI GGATC 3 cut(s) 224, 532, 2189
AcoI YGGCCR 2 cut(s) 300, 2087
AcsI RAATTY 8 cut(s) 989, 1549, 1600, 2325, 2369, 2840, 2900, 2947
AcuI CTGAAG 4 cut(s) 33, 1000, 1725, 2357
AcvI CACGTG 2 cut(s) 724, 1904
AcyI GRCGYC 1 cut(s) 1411
AdeI CACNNNGTG 2 cut(s) 727, 1907
AfaI GTAC 5 cut(s) 172, 326, 731, 1519, 2518
AfiI CCNNNNNNNGG 9 cut(s) 779, 780, 1249, 1264, 1643, 1889, 1994, 2398, 2449
AflII CTTAAG 2 cut(s) 1478, 2384
AflIII ACRYGT 5 cut(s) 107, 294, 369, 1588, 2138
AjiI CACGTC 2 cut(s) 297, 372
AjnI CCWGG 3 cut(s) 1945, 1993, 2572
AleI CACNNNNGTG 1 cut(s) 888
AloI GAACNNNNNNTCC 2 cut(s) 2128, 2160
Alw21I GWGCWC 2 cut(s) 228, 2283
Alw26I GTCTC 7 cut(s) 116, 289, 342, 378, 1277, 1771, 2274
Alw44I GTGCAC 1 cut(s) 2279
AlwI GGATC 3 cut(s) 224, 532, 2189
AlwNI CAGNNNCTG 1 cut(s) 2131
ApaLI GTGCAC 1 cut(s) 2279
ApeKI GCWGC 9 cut(s) 712, 754, 757, 844, 2059, 2459, 2549, 2864, 2909
ApoI RAATTY 8 cut(s) 989, 1549, 1600, 2325, 2369, 2840, 2900, 2947
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AseI ATTAAT 1 cut(s) 2424
Asp700I GAANNNNTTC 2 cut(s) 1788, 2922
Asp718I GGTACC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 267, 292
AspS9I GGNCC 5 cut(s) 163, 197, 938, 1267, 1404
AsuC2I CCSGG 3 cut(s) 304, 942, 1408
AsuHPI GGTGA 9 cut(s) 91, 373, 391, 565, 1364, 1601, 2018, 2326, 2531
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 2283
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 1 cut(s) 116
BbrPI CACGTG 2 cut(s) 724, 1904
BbsI GAAGAC 3 cut(s) 314, 1143, 2836
Bbv12I GWGCWC 2 cut(s) 228, 2283
BbvI GCAGC 9 cut(s) 724, 741, 769, 831, 2046, 2471, 2561, 2851, 2921
BccI CCATC 5 cut(s) 331, 637, 1343, 1456, 2728
BceAI ACGGC 2 cut(s) 110, 1545
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 3 cut(s) 1947, 1995, 2574
BcnI CCSGG 3 cut(s) 304, 942, 1408
BcoDI GTCTC 7 cut(s) 116, 289, 342, 378, 1277, 1771, 2274
BfmI CTRYAG 3 cut(s) 755, 845, 2960
BfrI CTTAAG 2 cut(s) 1478, 2384
BglII AGATCT 2 cut(s) 243, 2819
BlpI GCTNAGC 1 cut(s) 1556
BmcAI AGTACT 1 cut(s) 1519
Bme1390I CCNGG 6 cut(s) 304, 942, 1408, 1947, 1995, 2574
Bme18I GGWCC 1 cut(s) 163
BmgBI CACGTC 2 cut(s) 297, 372
BmgT120I GGNCC 5 cut(s) 163, 197, 938, 1267, 1404
BmiI GGNNCC 3 cut(s) 45, 172, 199
BmrFI CCNGG 6 cut(s) 304, 942, 1408, 1947, 1995, 2574
BmrI ACTGGG 3 cut(s) 456, 795, 2141
BmsI GCATC 2 cut(s) 802, 1207
BmuI ACTGGG 3 cut(s) 456, 795, 2141
BpiI GAAGAC 3 cut(s) 314, 1143, 2836
BpmI CTGGAG 2 cut(s) 2016, 2114
Bpu1102I GCTNAGC 1 cut(s) 1556
BpuEI CTTGAG 3 cut(s) 1685, 1769, 2256
BpuMI CCSGG 3 cut(s) 304, 942, 1408
Bsa29I ATCGAT 1 cut(s) 1363
BsaAI YACGTR 2 cut(s) 724, 1904
BsaBI GATNNNNATC 2 cut(s) 1359, 2532
BsaHI GRCGYC 1 cut(s) 1411
BsaI GGTCTC 1 cut(s) 1771
BsaJI CCNNGG 8 cut(s) 22, 166, 193, 249, 774, 1993, 2430, 2929
BsaWI WCCGGW 2 cut(s) 376, 2477
BsaXI ACNNNNNCTCC 6 cut(s) 1055, 1085, 1848, 1878, 2619, 2649
Bsc4I CCNNNNNNNGG 9 cut(s) 779, 780, 1249, 1264, 1643, 1889, 1994, 2398, 2449
Bse118I RCCGGY 2 cut(s) 134, 905
Bse1I ACTGG 7 cut(s) 451, 790, 1813, 2097, 2136, 2443, 2851
Bse3DI GCAATG 4 cut(s) 1050, 1117, 1188, 1299
Bse8I GATNNNNATC 2 cut(s) 1359, 2532
BseBI CCWGG 3 cut(s) 1947, 1995, 2574
BseCI ATCGAT 1 cut(s) 1363
BseDI CCNNGG 8 cut(s) 22, 166, 193, 249, 774, 1993, 2430, 2929
BseGI GGATG 4 cut(s) 436, 457, 1354, 2743
BseJI GATNNNNATC 2 cut(s) 1359, 2532
BseLI CCNNNNNNNGG 9 cut(s) 779, 780, 1249, 1264, 1643, 1889, 1994, 2398, 2449
BseMI GCAATG 4 cut(s) 1050, 1117, 1188, 1299
BseMII CTCAG 6 cut(s) 486, 494, 669, 1272, 1543, 1547
BseNI ACTGG 7 cut(s) 451, 790, 1813, 2097, 2136, 2443, 2851
BseRI GAGGAG 2 cut(s) 69, 623
BseSI GKGCMC 1 cut(s) 2283
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 9 cut(s) 724, 741, 769, 831, 2046, 2471, 2561, 2851, 2921
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 2144
Bsh1236I CGCG 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 170
BshVI ATCGAT 1 cut(s) 1363
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 2 cut(s) 228, 2283
BsiSI CCGG 9 cut(s) 41, 135, 303, 377, 632, 906, 941, 1407, 2478
BslFI GGGAC 4 cut(s) 113, 796, 1968, 2006
BslI CCNNNNNNNGG 9 cut(s) 779, 780, 1249, 1264, 1643, 1889, 1994, 2398, 2449
BsmAI GTCTC 7 cut(s) 116, 289, 342, 378, 1277, 1771, 2274
BsmBI CGTCTC 2 cut(s) 289, 378
BsmFI GGGAC 4 cut(s) 113, 796, 1968, 2006
BsmI GAATGC 6 cut(s) 188, 475, 516, 676, 2187, 2681
Bso31I GGTCTC 1 cut(s) 1771
Bsp1286I GDGCHC 2 cut(s) 228, 2283
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
Bsp1720I GCTNAGC 1 cut(s) 1556
BspCNI CTCAG 6 cut(s) 487, 495, 670, 1273, 1542, 1548
BspDI ATCGAT 1 cut(s) 1363
BspFNI CGCG 1 cut(s) 329
BspHI TCATGA 1 cut(s) 2320
BspLI GGNNCC 3 cut(s) 45, 172, 199
BspMAI CTGCAG 2 cut(s) 759, 849
BspPI GGATC 3 cut(s) 224, 532, 2189
BspT107I GGYRCC 1 cut(s) 170
BspTI CTTAAG 2 cut(s) 1478, 2384
BspTNI GGTCTC 1 cut(s) 1771
BsrBI CCGCTC 1 cut(s) 1398
BsrDI GCAATG 4 cut(s) 1050, 1117, 1188, 1299
BsrFI RCCGGY 2 cut(s) 134, 905
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 7 cut(s) 451, 790, 1813, 2097, 2136, 2443, 2851
BssAI RCCGGY 2 cut(s) 134, 905
BssECI CCNNGG 8 cut(s) 22, 166, 193, 249, 774, 1993, 2430, 2929
BssMI GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
BssNI GRCGYC 1 cut(s) 1411
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 22, 193
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 3 cut(s) 1947, 1995, 2574
Bst4CI ACNGT 6 cut(s) 170, 1321, 1940, 1964, 2393, 2719
Bst6I CTCTTC 2 cut(s) 1018, 2232
BstACI GRCGYC 1 cut(s) 1411
BstAFI CTTAAG 2 cut(s) 1478, 2384
BstAPI GCANNNNNTGC 2 cut(s) 2555, 2771
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 724, 1904
BstC8I GCNNGC 8 cut(s) 136, 256, 1152, 1311, 2057, 2457, 2464, 2616
BstDEI CTNAG 8 cut(s) 495, 503, 678, 1056, 1281, 1529, 1556, 2051
BstDSI CCRYGG 3 cut(s) 166, 249, 774
BstENI CCTNNNNNAGG 4 cut(s) 1247, 1262, 1641, 1887
BstF5I GGATG 4 cut(s) 436, 457, 1354, 2743
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 2 cut(s) 267, 292
BstKTI GATC 7 cut(s) 232, 246, 527, 2197, 2536, 2585, 2822
BstMAI GTCTC 7 cut(s) 116, 289, 342, 378, 1277, 1771, 2274
BstMBI GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
BstMCI CGRYCG 1 cut(s) 303
BstNI CCWGG 3 cut(s) 1947, 1995, 2574
BstNSI RCATGY 4 cut(s) 111, 1309, 1592, 2018
BstSCI CCNGG 6 cut(s) 302, 940, 1406, 1945, 1993, 2572
BstSFI CTRYAG 3 cut(s) 755, 845, 2960
BstSLI GKGCMC 1 cut(s) 2283
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 9 cut(s) 724, 741, 769, 831, 2046, 2471, 2561, 2851, 2921
BstV2I GAAGAC 3 cut(s) 314, 1143, 2836
BstX2I RGATCY 3 cut(s) 243, 2194, 2819
BstYI RGATCY 3 cut(s) 243, 2194, 2819
BstZI CGGCCG 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 1363
BsuTUI ATCGAT 1 cut(s) 1363
BtgI CCRYGG 3 cut(s) 166, 249, 774
BtgZI GCGATG 1 cut(s) 350
BtrI CACGTC 2 cut(s) 297, 372
BtsCI GGATG 4 cut(s) 436, 457, 1354, 2743
BtsI GCAGTG 2 cut(s) 2127, 2258
BtsIMutI CAGTG 6 cut(s) 675, 1936, 2090, 2127, 2258, 2724
Cac8I GCNNGC 8 cut(s) 136, 256, 1152, 1311, 2057, 2457, 2464, 2616
CaiI CAGNNNCTG 1 cut(s) 2131
CciI TCATGA 1 cut(s) 2320
CfoI GCGC 2 cut(s) 267, 292
Cfr10I RCCGGY 2 cut(s) 134, 905
Cfr13I GGNCC 5 cut(s) 163, 197, 938, 1267, 1404
ClaI ATCGAT 1 cut(s) 1363
CseI GACGC 1 cut(s) 1400
Csp6I GTAC 5 cut(s) 171, 325, 730, 1518, 2517
CspCI CAANNNNNGTGG 4 cut(s) 664, 699, 889, 924
CviQI GTAC 5 cut(s) 171, 325, 730, 1518, 2517
DdeI CTNAG 8 cut(s) 495, 503, 678, 1056, 1281, 1529, 1556, 2051
DpnI GATC 7 cut(s) 231, 245, 526, 2196, 2535, 2584, 2821
DpnII GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
DraIII CACNNNGTG 2 cut(s) 727, 1907
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 300, 2087
EagI CGGCCG 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 1018, 2232
EarI CTCTTC 2 cut(s) 1018, 2232
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 22, 193
Eco147I AGGCCT 2 cut(s) 1257, 2154
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 1771
Eco32I GATATC 1 cut(s) 2043
Eco47I GGWCC 1 cut(s) 163
Eco52I CGGCCG 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 4 cut(s) 33, 1000, 1725, 2357
Eco72I CACGTG 2 cut(s) 724, 1904
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 4 cut(s) 1247, 1262, 1641, 1887
EcoO109I RGGNCCY 2 cut(s) 197, 1267
EcoRI GAATTC 1 cut(s) 1600
EcoRII CCWGG 3 cut(s) 1945, 1993, 2572
EcoRV GATATC 1 cut(s) 2043
EcoT14I CCWWGG 2 cut(s) 22, 193
EcoT22I ATGCAT 1 cut(s) 2016
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 22, 193
Esp3I CGTCTC 2 cut(s) 289, 378
FalI AAGNNNNNCTT 6 cut(s) 1049, 1081, 1256, 1288, 1589, 1621
FaqI GGGAC 4 cut(s) 113, 796, 1968, 2006
FauI CCCGC 3 cut(s) 139, 193, 2448
FblI GTMKAC 1 cut(s) 2697
FokI GGATG 4 cut(s) 443, 464, 1361, 2750
FriOI GRGCYC 1 cut(s) 228
GlaI GCGC 2 cut(s) 266, 291
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 2 cut(s) 2016, 2114
HapII CCGG 9 cut(s) 41, 135, 303, 377, 632, 906, 941, 1407, 2478
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 2 cut(s) 267, 292
Hin1I GRCGYC 1 cut(s) 1411
Hin6I GCGC 2 cut(s) 265, 290
HinP1I GCGC 2 cut(s) 265, 290
HindIII AAGCTT 3 cut(s) 72, 1058, 2411
HpaII CCGG 9 cut(s) 41, 135, 303, 377, 632, 906, 941, 1407, 2478
HphI GGTGA 9 cut(s) 91, 373, 391, 565, 1364, 1601, 2018, 2326, 2531
Hpy166II GTNNAC 9 cut(s) 166, 730, 732, 805, 1034, 1499, 1967, 2281, 2698
Hpy8I GTNNAC 9 cut(s) 166, 730, 732, 805, 1034, 1499, 1967, 2281, 2698
Hpy99I CGWCG 3 cut(s) 151, 301, 1416
HpyCH4III ACNGT 6 cut(s) 170, 1321, 1940, 1964, 2393, 2719
HpyCH4IV ACGT 5 cut(s) 296, 371, 723, 1903, 2140
HpyF3I CTNAG 8 cut(s) 495, 503, 678, 1056, 1281, 1529, 1556, 2051
HpySE526I ACGT 5 cut(s) 296, 371, 723, 1903, 2140
Hsp92I GRCGYC 1 cut(s) 1411
HspAI GCGC 2 cut(s) 265, 290
KpnI GGTACC 1 cut(s) 174
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
LmnI GCTCC 4 cut(s) 43, 262, 1510, 1892
Lsp1109I GCAGC 9 cut(s) 724, 741, 769, 831, 2046, 2471, 2561, 2851, 2921
LweI GCATC 2 cut(s) 802, 1207
MaeII ACGT 5 cut(s) 296, 371, 723, 1903, 2140
MaeIII GTNAC 4 cut(s) 454, 719, 881, 2494
MalI GATC 7 cut(s) 231, 245, 526, 2196, 2535, 2584, 2821
MbiI CCGCTC 1 cut(s) 1398
MboI GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
MfeI CAATTG 1 cut(s) 2600
MflI RGATCY 3 cut(s) 243, 2194, 2819
MhlI GDGCHC 2 cut(s) 228, 2283
MlyI GAGTC 4 cut(s) 167, 281, 742, 1521
MmeI TCCRAC 3 cut(s) 170, 415, 2857
Mph1103I ATGCAT 1 cut(s) 2016
MroNI GCCGGC 1 cut(s) 134
MroXI GAANNNNTTC 2 cut(s) 1788, 2922
MslI CAYNNNNRTG 3 cut(s) 888, 1193, 2424
MspA1I CMGCKG 5 cut(s) 754, 1334, 1583, 2038, 2864
MspCI CTTAAG 2 cut(s) 1478, 2384
MspI CCGG 9 cut(s) 41, 135, 303, 377, 632, 906, 941, 1407, 2478
MspR9I CCNGG 6 cut(s) 304, 942, 1408, 1947, 1995, 2574
MunI CAATTG 1 cut(s) 2600
Mva1269I GAATGC 6 cut(s) 188, 475, 516, 676, 2187, 2681
MvaI CCWGG 3 cut(s) 1947, 1995, 2574
MvnI CGCG 1 cut(s) 329
NaeI GCCGGC 1 cut(s) 136
NciI CCSGG 3 cut(s) 304, 942, 1408
NdeII GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
NgoMIV GCCGGC 1 cut(s) 134
NlaIV GGNNCC 3 cut(s) 45, 172, 199
NmeAIII GCCGAG 4 cut(s) 266, 635, 2455, 2954
NmuCI GTSAC 3 cut(s) 454, 719, 881
NsiI ATGCAT 1 cut(s) 2016
NspI RCATGY 4 cut(s) 111, 1309, 1592, 2018
OliI CACNNNNGTG 1 cut(s) 888
PagI TCATGA 1 cut(s) 2320
PceI AGGCCT 2 cut(s) 1257, 2154
PciI ACATGT 2 cut(s) 107, 1588
PcsI WCGNNNNNNNCGW 4 cut(s) 123, 293, 464, 470
PctI GAATGC 6 cut(s) 188, 475, 516, 676, 2187, 2681
PdiI GCCGGC 1 cut(s) 136
PdmI GAANNNNTTC 2 cut(s) 1788, 2922
PfeI GAWTC 7 cut(s) 11, 499, 1688, 2167, 2317, 2354, 2711
PfoI TCCNGGA 1 cut(s) 2572
PleI GAGTC 4 cut(s) 166, 280, 742, 1521
PmaCI CACGTG 2 cut(s) 724, 1904
PmlI CACGTG 2 cut(s) 724, 1904
PpsI GAGTC 4 cut(s) 166, 280, 742, 1521
Ppu21I YACGTR 2 cut(s) 724, 1904
PscI ACATGT 2 cut(s) 107, 1588
PshBI ATTAAT 1 cut(s) 2424
PsiI TTATAA 1 cut(s) 1004
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 1945, 1993, 2572
PspCI CACGTG 2 cut(s) 724, 1904
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 1945, 1993, 2572
PspN4I GGNNCC 3 cut(s) 45, 172, 199
PspPI GGNCC 5 cut(s) 163, 197, 938, 1267, 1404
PsrI GAACNNNNNNTAC 2 cut(s) 2010, 2042
PstI CTGCAG 2 cut(s) 759, 849
PstNI CAGNNNCTG 1 cut(s) 2131
PsuI RGATCY 3 cut(s) 243, 2194, 2819
PvuII CAGCTG 3 cut(s) 1334, 1583, 2864
RsaI GTAC 5 cut(s) 172, 326, 731, 1519, 2518
RsaNI GTAC 5 cut(s) 171, 325, 730, 1518, 2517
RseI CAYNNNNRTG 3 cut(s) 888, 1193, 2424
SacI GAGCTC 1 cut(s) 228
Sau3AI GATC 7 cut(s) 229, 243, 524, 2194, 2533, 2582, 2819
Sau96I GGNCC 5 cut(s) 163, 197, 938, 1267, 1404
ScaI AGTACT 1 cut(s) 1519
SchI GAGTC 4 cut(s) 167, 281, 742, 1521
ScrFI CCNGG 6 cut(s) 304, 942, 1408, 1947, 1995, 2574
SduI GDGCHC 2 cut(s) 228, 2283
SfaNI GCATC 2 cut(s) 802, 1207
SfcI CTRYAG 3 cut(s) 755, 845, 2960
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 3 cut(s) 888, 1193, 2424
SmlI CTYRAG 5 cut(s) 1478, 1664, 1748, 2271, 2384
SmoI CTYRAG 5 cut(s) 1478, 1664, 1748, 2271, 2384
SseBI AGGCCT 2 cut(s) 1257, 2154
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 2 cut(s) 1257, 2154
StyD4I CCNGG 6 cut(s) 302, 940, 1406, 1945, 1993, 2572
StyI CCWWGG 2 cut(s) 22, 193
TaaI ACNGT 6 cut(s) 170, 1321, 1940, 1964, 2393, 2719
TaiI ACGT 5 cut(s) 299, 374, 726, 1906, 2143
TaqI TCGA 9 cut(s) 179, 222, 228, 346, 426, 561, 668, 860, 1363
TaqII GACCGA 1 cut(s) 151
TatI WGTACW 2 cut(s) 729, 1517
TauI GCSGC 3 cut(s) 631, 2038, 2089
TfiI GAWTC 7 cut(s) 11, 499, 1688, 2167, 2317, 2354, 2711
TscAI CASTG 6 cut(s) 682, 1943, 2097, 2127, 2265, 2724
TseFI GTSAC 3 cut(s) 454, 719, 881
TseI GCWGC 9 cut(s) 712, 754, 757, 844, 2059, 2459, 2549, 2864, 2909
Tsp45I GTSAC 3 cut(s) 454, 719, 881
TspGWI ACGGA 3 cut(s) 222, 238, 749
TspRI CASTG 6 cut(s) 682, 1943, 2097, 2127, 2265, 2724
Vha464I CTTAAG 2 cut(s) 1478, 2384
VneI GTGCAC 1 cut(s) 2279
VpaK11BI GGWCC 1 cut(s) 163
VspI ATTAAT 1 cut(s) 2424
XagI CCTNNNNNAGG 4 cut(s) 1247, 1262, 1641, 1887
XapI RAATTY 8 cut(s) 989, 1549, 1600, 2325, 2369, 2840, 2900, 2947
XceI RCATGY 4 cut(s) 111, 1309, 1592, 2018
XmiI GTMKAC 1 cut(s) 2697
XmnI GAANNNNTTC 2 cut(s) 1788, 2922
ZrmI AGTACT 1 cut(s) 1519
Zsp2I ATGCAT 1 cut(s) 2016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.