AT2G15680

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015472
Physical Location & Seq
Forward (+)
6830995 .. 6831641
647 bp
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UTR
Exon/CDS
Intron
AT2G15680.1

Sequence Viewer

Length: 564 bp
ATGTCAAACGTGAGTTTTCTTGAGTTGCAGTACAAGCTCTCCAAGAACAAGATGTTGAGGAAGCCTTCAAGGATGTTCTCTAGAGATAGACAATCCTCAGGGCTATCTTCACCTGGACCAGGAGGCTTCTCTCAGCCTTCTGTGAATGAGATGAGACGTGTTTTCAGCAGGTTTGATTTGGATAAAGACGGGAAAATCTCTCAGACTGAGTACAAGGTGGTGCTGAGAGCGCTAGGACAAGAGCGGGCGATCGAGGATGTGCCTAAGATCTTTAAGGCTGTGGATCTGGACGGTGATGGGTTTATTGATTTCAGGGAGTTTATTGATGCATACAAGAGAAGTGGTGGGATTAGGTCTTCGGATATACGAAATTCTTTCTGGACTTTTGATTTGAACGGCGATGGGAAGATAAGCGCAGAGGAAGTGATGTCGGTTCTGTGGAAGCTTGGTGAGAGATGTAGCTTAGAGGACTGCAACAGGATGGTTAGAGCTGTTGATGCAGATGGTGATGGATTGGTTAATATGGAAGAGTTCATCAAAATGATGTCTTCCAACAATGTCTAA

Protein Analysis

187

Amino Acids

21.22

Weight (kDa)

6.4

Isoelectric Point (pI)

57.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 49 - 112 9e-13 EF-hand domain pair
EF-hand_1 PF00036 50 - 78 4.5e-06 EF hand domain
EF-hand_6 PF13405 50 - 79 3.1e-06 EF-hand domain
EF-hand_1 PF00036 89 - 113 3.3e-07 EF hand domain
EF-hand_7 PF13499 120 - 183 2e-13 EF-hand domain pair
EF-hand_1 PF00036 121 - 149 9.6e-06 EF hand domain
EF-hand_8 PF13833 136 - 182 8.8e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 244
AciI CCGC 1 cut(s) 244
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 370
AfaI GTAC 2 cut(s) 32, 212
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 2 cut(s) 69, 394
AjiI CACGTC 1 cut(s) 158
AjnI CCWGG 2 cut(s) 112, 118
AluBI AGCT 4 cut(s) 37, 445, 462, 491
AluI AGCT 4 cut(s) 37, 445, 462, 491
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 1 cut(s) 291
Aor51HI AGCGCT 1 cut(s) 231
ApoI RAATTY 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
AspLEI GCGC 2 cut(s) 232, 416
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 4 cut(s) 102, 305, 461, 518
AvaII GGWCC 1 cut(s) 116
AxyI CCTNAGG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 348, 540
BccI CCATC 5 cut(s) 290, 395, 475, 497, 503
BceAI ACGGC 1 cut(s) 412
BciT130I CCWGG 2 cut(s) 114, 120
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 2 cut(s) 81, 233
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 159
BglII AGATCT 1 cut(s) 267
Bme1390I CCNGG 2 cut(s) 114, 120
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 116
BmrFI CCNGG 2 cut(s) 114, 120
BmsI GCATC 2 cut(s) 316, 487
BpiI GAAGAC 2 cut(s) 348, 540
BpuEI CTTGAG 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse21I CCTNAGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 114, 120
BseGI GGATG 3 cut(s) 78, 262, 486
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 5 cut(s) 111, 146, 198, 215, 215
Bsh1285I CGRYCG 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 249, 267, 283
BspACI CCGC 1 cut(s) 244
BspCNI CTCAG 5 cut(s) 110, 145, 199, 214, 216
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 1 cut(s) 291
BsrBI CCGCTC 1 cut(s) 244
BssMI GATC 3 cut(s) 249, 267, 283
Bst2UI CCWGG 2 cut(s) 114, 120
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 7 cut(s) 97, 132, 201, 207, 224, 264, 463
BstENI CCTNNNNNAGG 1 cut(s) 117
BstF5I GGATG 3 cut(s) 78, 262, 486
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 2 cut(s) 232, 416
BstKTI GATC 3 cut(s) 252, 270, 286
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 3 cut(s) 249, 267, 283
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 3 cut(s) 34, 229, 497
BstNI CCWGG 2 cut(s) 114, 120
BstSCI CCNGG 2 cut(s) 112, 118
BstV2I GAAGAC 2 cut(s) 348, 540
BstX2I RGATCY 2 cut(s) 267, 283
BstYI RGATCY 2 cut(s) 267, 283
Bsu36I CCTNAGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 414
BtrI CACGTC 1 cut(s) 158
BtsCI GGATG 3 cut(s) 78, 262, 486
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 246
CfoI GCGC 2 cut(s) 232, 416
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 31, 211
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviJI RGCY 9 cut(s) 37, 64, 103, 126, 136, 278, 445, 462, 491
CviKI_1 RGCY 9 cut(s) 37, 64, 103, 126, 136, 278, 445, 462, 491
CviQI GTAC 2 cut(s) 31, 211
DdeI CTNAG 7 cut(s) 97, 132, 201, 207, 224, 264, 463
DpnI GATC 3 cut(s) 251, 269, 285
DpnII GATC 3 cut(s) 249, 267, 283
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 116
Eco47III AGCGCT 1 cut(s) 231
Eco81I CCTNAGG 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 112, 118
EcoT22I ATGCAT 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 148
FaiI YATR 3 cut(s) 331, 365, 524
FauI CCCGC 1 cut(s) 237
FokI GGATG 3 cut(s) 85, 269, 493
FspBI CTAG 2 cut(s) 81, 233
GlaI GCGC 2 cut(s) 231, 415
HaeII RGCGCY 1 cut(s) 233
HhaI GCGC 2 cut(s) 232, 416
Hin6I GCGC 2 cut(s) 230, 414
HinP1I GCGC 2 cut(s) 230, 414
HindIII AAGCTT 1 cut(s) 443
HphI GGTGA 4 cut(s) 102, 305, 461, 518
Hpy188I TCNGA 2 cut(s) 204, 361
Hpy188III TCNNGA 4 cut(s) 20, 81, 287, 379
HpyAV CCTTC 2 cut(s) 75, 147
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 2 cut(s) 9, 157
HpyCH4V TGCA 4 cut(s) 28, 329, 474, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 229, 497
HpyF3I CTNAG 7 cut(s) 97, 132, 201, 207, 224, 264, 463
HpySE526I ACGT 2 cut(s) 9, 157
HspAI GCGC 2 cut(s) 230, 414
Kzo9I GATC 3 cut(s) 249, 267, 283
LweI GCATC 2 cut(s) 316, 487
MaeI CTAG 2 cut(s) 81, 233
MaeII ACGT 2 cut(s) 9, 157
MalI GATC 3 cut(s) 251, 269, 285
MbiI CCGCTC 1 cut(s) 244
MboI GATC 3 cut(s) 249, 267, 283
MboII GAAGA 5 cut(s) 99, 348, 418, 539, 540
MflI RGATCY 2 cut(s) 267, 283
MluCI AATT 1 cut(s) 370
MnlI CCTC 6 cut(s) 51, 106, 116, 247, 412, 460
Mph1103I ATGCAT 1 cut(s) 331
MseI TTAA 2 cut(s) 273, 519
MslI CAYNNNNRTG 1 cut(s) 539
MspR9I CCNGG 2 cut(s) 114, 120
MvaI CCWGG 2 cut(s) 114, 120
MwoI GCNNNNNNNGC 3 cut(s) 34, 229, 497
NdeII GATC 3 cut(s) 249, 267, 283
NsiI ATGCAT 1 cut(s) 331
Ple19I CGATCG 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 112, 118
PspGI CCWGG 2 cut(s) 112, 118
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 2 cut(s) 267, 283
PvuI CGATCG 1 cut(s) 252
RsaI GTAC 2 cut(s) 32, 212
RsaNI GTAC 2 cut(s) 31, 211
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 2 cut(s) 273, 519
Sau3AI GATC 3 cut(s) 249, 267, 283
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 114, 120
SfaNI GCATC 2 cut(s) 316, 487
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 1 cut(s) 370
SsiI CCGC 1 cut(s) 244
SspMI CTAG 2 cut(s) 81, 233
StyD4I CCNGG 2 cut(s) 112, 118
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 2 cut(s) 12, 160
TaqI TCGA 1 cut(s) 252
TasI AATT 1 cut(s) 370
TatI WGTACW 2 cut(s) 30, 210
Tru1I TTAA 2 cut(s) 273, 519
Tru9I TTAA 2 cut(s) 273, 519
TspDTI ATGAA 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 370
XbaI TCTAGA 1 cut(s) 80
XspI CTAG 2 cut(s) 81, 233
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.