MD05G1080100.v1.1

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015472
Physical Location & Seq
Reverse (-)
16795179 .. 16795733
555 bp
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UTR
Exon/CDS
Intron
MD05G1080100.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGCGAATGCAAACTTCCTCGACTTCCAGTACAACCTATCAAAGAGGAAGTTTCTGCGCAAACCATCTCAGATGTTTTCCTTTCGAGACAGGCAATACTCCGGCATAGCACCTACTTTCGAGCCAAATCCCAATGAGATGAAGAAAGTATTTGAAAAGTTTGACTCCAACAAAGACGGGAAGATATCTCAGGCGGAGTACAAGGCGATACTGAGAGCATTGGGAAAGGGAAACATGATTGGAGAAGTGCCGAAGATTTTCAAGTTTGTTGATCTGGATGAGGATGGCTTTATCAATTTCAAGGAGTTCATGGAAGTACACAAGAAGGGAGGGATTCGAACGATGGACATCCAGAATGCTTTTCGGATGTTTGATTTGAACGGCGATGGAAAGATAAGTGCAGAAGAAGTTATGGAAGTGCTGAGGGGGTTGGAGGAGAGGTGCAACCTTGAAGACTGCCGGCGGATGGTGAGAGCGGTTGACACTGATGGGGATGGTATGGTTGACCTAGATGAGTTCATGACCATGATGACTCGTTCCATGAGACCAGCATAG

Protein Analysis

185

Amino Acids

21.35

Weight (kDa)

5.99

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 46 - 74 2.8e-07 EF hand domain
EF-hand_7 PF13499 46 - 106 3e-11 EF-hand domain pair
EF-hand_6 PF13405 46 - 75 3.3e-07 EF-hand domain
EF-hand_5 PF13202 48 - 70 4.7e-06 EF hand
EF-hand_7 PF13499 115 - 178 7.4e-17 EF-hand domain pair
EF-hand_1 PF00036 116 - 143 3.7e-08 EF hand domain
EF-hand_6 PF13405 116 - 143 2e-07 EF-hand domain
EF-hand_5 PF13202 120 - 140 1.2e-07 EF hand
EF-hand_8 PF13833 144 - 179 3.2e-07 EF-hand domain pair
EF-hand_1 PF00036 152 - 179 4.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 476
AciI CCGC 3 cut(s) 194, 463, 476
AfaI GTAC 3 cut(s) 32, 200, 318
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 5 cut(s) 155, 262, 301, 379, 452
Alw26I GTCTC 2 cut(s) 81, 538
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 481
AsuII TTCGAA 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 422
BccI CCATC 7 cut(s) 73, 278, 337, 380, 460, 482, 488
BceAI ACGGC 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 81, 538
BfaI CTAG 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse118I RCCGGY 1 cut(s) 459
Bse1I ACTGG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 347
BseGI GGATG 6 cut(s) 283, 289, 348, 372, 471, 499
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 4 cut(s) 83, 203, 203, 413
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 420
BsiSI CCGG 2 cut(s) 102, 460
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 81, 538
BsmI GAATGC 2 cut(s) 13, 361
Bso31I GGTCTC 1 cut(s) 538
Bsp119I TTCGAA 1 cut(s) 337
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 3 cut(s) 194, 463, 476
BspCNI CTCAG 4 cut(s) 82, 202, 204, 414
BspHI TCATGA 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 538
BsrBI CCGCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 459
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 459
BssMI GATC 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 461
BstDEI CTNAG 4 cut(s) 69, 189, 212, 422
BstF5I GGATG 6 cut(s) 283, 289, 348, 372, 471, 499
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 2 cut(s) 81, 538
BstMBI GATC 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 6 cut(s) 283, 289, 348, 372, 471, 499
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 1 cut(s) 461
CciI TCATGA 1 cut(s) 519
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 459
Csp6I GTAC 3 cut(s) 31, 199, 317
CviAII CATG 5 cut(s) 235, 310, 520, 526, 541
CviJI RGCY 2 cut(s) 124, 288
CviKI_1 RGCY 2 cut(s) 124, 288
CviQI GTAC 3 cut(s) 31, 199, 317
DdeI CTNAG 4 cut(s) 69, 189, 212, 422
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EciI GGCGGA 2 cut(s) 209, 478
Eco31I GGTCTC 1 cut(s) 538
Eco32I GATATC 1 cut(s) 186
EcoRV GATATC 1 cut(s) 186
FaeI CATG 5 cut(s) 238, 313, 523, 529, 544
FaiI YATR 9 cut(s) 107, 236, 311, 413, 500, 521, 527, 542, 553
FatI CATG 5 cut(s) 234, 309, 519, 525, 540
FokI GGATG 6 cut(s) 290, 296, 335, 379, 478, 506
FspBI CTAG 1 cut(s) 509
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
HapII CCGG 2 cut(s) 102, 460
HhaI GCGC 1 cut(s) 60
Hin1II CATG 5 cut(s) 238, 313, 523, 529, 544
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 481, 505
HindII GTYRAC 2 cut(s) 481, 505
HinfI GANTC 3 cut(s) 164, 334, 532
HpaII CCGG 2 cut(s) 102, 460
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 3 cut(s) 319, 481, 505
Hpy188I TCNGA 2 cut(s) 72, 366
Hpy188III TCNNGA 4 cut(s) 86, 275, 352, 520
Hpy8I GTNNAC 3 cut(s) 319, 481, 505
HpyAV CCTTC 1 cut(s) 319
HpyCH4V TGCA 3 cut(s) 11, 401, 444
HpyF3I CTNAG 4 cut(s) 69, 189, 212, 422
Hsp92II CATG 5 cut(s) 238, 313, 523, 529, 544
HspAI GCGC 1 cut(s) 58
KroI GCCGGC 1 cut(s) 459
KroNI GCCGGC 1 cut(s) 461
Kzo9I GATC 1 cut(s) 271
LpnPI CCDG 7 cut(s) 41, 76, 115, 176, 260, 365, 473
MaeI CTAG 1 cut(s) 509
MalI GATC 1 cut(s) 273
MbiI CCGCTC 1 cut(s) 476
MboI GATC 1 cut(s) 271
MboII GAAGA 5 cut(s) 154, 193, 265, 416, 464
MluCI AATT 1 cut(s) 295
MlyI GAGTC 2 cut(s) 158, 526
MmeI TCCRAC 2 cut(s) 192, 411
MnlI CCTC 7 cut(s) 29, 39, 274, 323, 417, 427, 432
MroNI GCCGGC 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 2 cut(s) 102, 460
Mva1269I GAATGC 2 cut(s) 13, 361
NaeI GCCGGC 1 cut(s) 461
NdeII GATC 1 cut(s) 271
NgoMIV GCCGGC 1 cut(s) 459
NlaIII CATG 5 cut(s) 238, 313, 523, 529, 544
NsbI TGCGCA 1 cut(s) 59
NspV TTCGAA 1 cut(s) 337
PagI TCATGA 1 cut(s) 519
PctI GAATGC 2 cut(s) 13, 361
PdiI GCCGGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 334
PleI GAGTC 2 cut(s) 158, 526
PpsI GAGTC 2 cut(s) 158, 526
RsaI GTAC 3 cut(s) 32, 200, 318
RsaNI GTAC 3 cut(s) 31, 199, 317
RseI CAYNNNNRTG 1 cut(s) 524
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 2 cut(s) 158, 526
SetI ASST 5 cut(s) 39, 115, 443, 450, 510
SfuI TTCGAA 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 524
Sse9I AATT 1 cut(s) 295
SsiI CCGC 3 cut(s) 194, 463, 476
SspMI CTAG 1 cut(s) 509
TaqI TCGA 4 cut(s) 21, 85, 120, 337
TasI AATT 1 cut(s) 295
TatI WGTACW 3 cut(s) 30, 198, 316
TfiI GAWTC 1 cut(s) 334
TscAI CASTG 1 cut(s) 490
TspDTI ATGAA 3 cut(s) 155, 298, 508
TspRI CASTG 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.