pycom05g07320

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015472
Physical Location & Seq
Reverse (-)
10314890 .. 10315444
555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07320.1

Sequence Viewer

Length: 555 bp
ATGGCGAATGCAAACTTCCTCGACTTCCAGTACAACCTCTCAAAGAGGAAGTTTCTGCGCAAACCATCTCAGATGTTTTCCTTTCGAGACAGACAAAACTCCGGCATAGCACCTACTTTCGAGCCAAATCCCAATGAGATGAAGAAAGTATTTGAAAAGTTCGACTCCAACAAAGATGGGAAGATATCTCAGGCAGAGTACAAGGCGATACTGAGAGCACTGGGAAAGGGAAACATGATTGGAGAAGTGCCGAAGATTTTCAAGATTGTTGATCTGGATGGGGATGACTTTATCAATTTCGAGGAGTTCATGGAAGTACACAAGAAGGGAGGGATTCGAACGATGGACATCCAGGATGCTTTTCGGACGTTTGATTTGAACGGCGATGGAAAGATAAGTGCAGAAGAAGTTATGGAAGTGCTGAGGAGGTTGGGGGAGAGGTGCATCCTTGAAGACTGCCGGCGGATGGTGAGAGCGGTTGACACTGATGGGGATGGTATGGTTGACCTAGATGAGTTCATGAACATGATGACTCGTTCCATGAGACCAGCATAG

Protein Analysis

185

Amino Acids

21.26

Weight (kDa)

5.63

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 46 - 74 2.8e-07 EF hand domain
EF-hand_7 PF13499 46 - 106 2.1e-12 EF-hand domain pair
EF-hand_6 PF13405 46 - 75 3.3e-07 EF-hand domain
EF-hand_5 PF13202 48 - 70 4.7e-06 EF hand
EF-hand_7 PF13499 115 - 178 6.9e-16 EF-hand domain pair
EF-hand_1 PF00036 116 - 144 4.8e-08 EF hand domain
EF-hand_6 PF13405 116 - 145 7.9e-08 EF-hand domain
EF-hand_5 PF13202 117 - 140 2.4e-08 EF hand
EF-hand_8 PF13833 143 - 179 6.9e-07 EF-hand domain pair
EF-hand_1 PF00036 152 - 179 4.8e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 476
AciI CCGC 2 cut(s) 463, 476
AfaI GTAC 3 cut(s) 32, 200, 318
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 4 cut(s) 155, 262, 379, 452
AjnI CCWGG 1 cut(s) 351
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 81, 538
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 481
AsuII TTCGAA 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 220
BbvCI CCTCAGC 1 cut(s) 422
BccI CCATC 8 cut(s) 73, 170, 272, 337, 380, 460, 482, 488
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 81, 538
BfaI CTAG 1 cut(s) 509
Bme1390I CCNGG 1 cut(s) 353
BmrFI CCNGG 1 cut(s) 353
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 346, 453
BmuI ACTGGG 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 538
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse118I RCCGGY 1 cut(s) 459
Bse1I ACTGG 2 cut(s) 28, 225
Bse8I GATNNNNATC 1 cut(s) 347
BseBI CCWGG 1 cut(s) 353
BseGI GGATG 7 cut(s) 283, 289, 348, 361, 444, 471, 499
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 4 cut(s) 83, 203, 203, 413
BseNI ACTGG 2 cut(s) 28, 225
BseRI GAGGAG 2 cut(s) 317, 439
BsgI GTGCAG 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 2 cut(s) 102, 460
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 81, 538
BsmI GAATGC 1 cut(s) 13
Bso31I GGTCTC 1 cut(s) 538
Bsp119I TTCGAA 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 2 cut(s) 463, 476
BspCNI CTCAG 4 cut(s) 82, 202, 204, 414
BspHI TCATGA 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 538
BsrBI CCGCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 459
BsrI ACTGG 2 cut(s) 28, 225
BssAI RCCGGY 1 cut(s) 459
BssMI GATC 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 461
BstDEI CTNAG 4 cut(s) 69, 189, 212, 422
BstF5I GGATG 7 cut(s) 283, 289, 348, 361, 444, 471, 499
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 2 cut(s) 81, 538
BstMBI GATC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstV2I GAAGAC 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 7 cut(s) 283, 289, 348, 361, 444, 471, 499
BtsIMutI CAGTG 2 cut(s) 218, 483
Cac8I GCNNGC 1 cut(s) 461
CciI TCATGA 1 cut(s) 519
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 459
Csp6I GTAC 3 cut(s) 31, 199, 317
CviAII CATG 5 cut(s) 235, 310, 520, 526, 541
CviJI RGCY 1 cut(s) 124
CviKI_1 RGCY 1 cut(s) 124
CviQI GTAC 3 cut(s) 31, 199, 317
DdeI CTNAG 4 cut(s) 69, 189, 212, 422
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EciI GGCGGA 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 538
Eco32I GATATC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 351
EcoRV GATATC 1 cut(s) 186
FaeI CATG 5 cut(s) 238, 313, 523, 529, 544
FaiI YATR 9 cut(s) 107, 236, 311, 413, 500, 521, 527, 542, 553
FatI CATG 5 cut(s) 234, 309, 519, 525, 540
FokI GGATG 7 cut(s) 290, 296, 335, 368, 431, 478, 506
FspBI CTAG 1 cut(s) 509
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
HapII CCGG 2 cut(s) 102, 460
HhaI GCGC 1 cut(s) 60
Hin1II CATG 5 cut(s) 238, 313, 523, 529, 544
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 481, 505
HindII GTYRAC 2 cut(s) 481, 505
HinfI GANTC 3 cut(s) 164, 334, 532
HpaII CCGG 2 cut(s) 102, 460
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 3 cut(s) 319, 481, 505
Hpy188I TCNGA 2 cut(s) 72, 366
Hpy188III TCNNGA 4 cut(s) 86, 262, 275, 520
Hpy8I GTNNAC 3 cut(s) 319, 481, 505
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 11, 401, 444
HpyF3I CTNAG 4 cut(s) 69, 189, 212, 422
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 5 cut(s) 238, 313, 523, 529, 544
HspAI GCGC 1 cut(s) 58
KroI GCCGGC 1 cut(s) 459
KroNI GCCGGC 1 cut(s) 461
Kzo9I GATC 1 cut(s) 271
LpnPI CCDG 8 cut(s) 41, 115, 176, 206, 260, 338, 365, 473
LweI GCATC 2 cut(s) 346, 453
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 368
MalI GATC 1 cut(s) 273
MbiI CCGCTC 1 cut(s) 476
MboI GATC 1 cut(s) 271
MboII GAAGA 5 cut(s) 154, 193, 265, 416, 464
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 1 cut(s) 295
MlyI GAGTC 2 cut(s) 158, 526
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 8 cut(s) 29, 39, 47, 295, 323, 417, 420, 432
MroNI GCCGGC 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 2 cut(s) 102, 460
MspR9I CCNGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 13
MvaI CCWGG 1 cut(s) 353
NaeI GCCGGC 1 cut(s) 461
NdeII GATC 1 cut(s) 271
NgoMIV GCCGGC 1 cut(s) 459
NlaIII CATG 5 cut(s) 238, 313, 523, 529, 544
NsbI TGCGCA 1 cut(s) 59
NspV TTCGAA 1 cut(s) 337
PagI TCATGA 1 cut(s) 519
PctI GAATGC 1 cut(s) 13
PdiI GCCGGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 351
PleI GAGTC 2 cut(s) 158, 526
PpsI GAGTC 2 cut(s) 158, 526
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
RsaI GTAC 3 cut(s) 32, 200, 318
RsaNI GTAC 3 cut(s) 31, 199, 317
RseI CAYNNNNRTG 1 cut(s) 524
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 2 cut(s) 158, 526
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 220
SetI ASST 6 cut(s) 39, 115, 371, 431, 443, 510
SfaNI GCATC 2 cut(s) 346, 453
SfuI TTCGAA 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 524
Sse9I AATT 1 cut(s) 295
SsiI CCGC 2 cut(s) 463, 476
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 351
TaiI ACGT 1 cut(s) 371
TaqI TCGA 6 cut(s) 21, 85, 120, 162, 300, 337
TasI AATT 1 cut(s) 295
TatI WGTACW 3 cut(s) 30, 198, 316
TfiI GAWTC 1 cut(s) 334
TscAI CASTG 2 cut(s) 225, 490
TspDTI ATGAA 4 cut(s) 155, 298, 508, 536
TspRI CASTG 2 cut(s) 225, 490
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.