FvH4_2g06140

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015472
Physical Location & Seq
Forward (+)
5101367 .. 5102384
1018 bp
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UTR
Exon/CDS
Intron
FvH4_2g06140.t1

Sequence Viewer

Length: 558 bp
ATGTCGAACATGAGCTTCCTTGATTTCCAGTACAACCTATCAAAACGGAAGTTTCTGCGCACACCAACTCGATTGTTTTCCTCCCGGGACAGACAAAACTCTGGCTTAACACCTGGTTTCGAGCCAAATCTCAACGAGATGAAGCGAGTCTTTGAGAAGTTTGACACCAACAAGGATGGGAAGATATCTCAGCAGGACTACAGGGCCATATTGAGAGCACTTGGAAAGGGAAGCATGGTTGGAGAAGTGCCAAAGATTTTCAAGCTTGTTGATTTGGACGGGGATGGGTTTATTAACTTCAAGGAGTTCATGGAAGTGCACAAGAAGTCAGGTGGGGTGAAAACAATAGACATCCAGAATGCTTTCCAAACATTTGACCTGAATGGGGATGGAAAAATAAGTGCAGAAGAAGTTATGGAAGTGTTGAAGAGGCTGGGAGAGAGGTGCAGCCTCGAAGAATGCCAGCGAATGGTAAGAGCGGTAGACACCGATGGGGATGGTATGGTCGACTTAGACGAGTTCATGACCATGATGACTCGATCCATGAGACGAGTTTAG

Protein Analysis

186

Amino Acids

21.27

Weight (kDa)

7.72

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 45 - 106 9.1e-13 EF-hand domain pair
EF-hand_1 PF00036 46 - 74 3.6e-07 EF hand domain
EF-hand_6 PF13405 46 - 75 1e-07 EF-hand domain
EF-hand_1 PF00036 85 - 105 4e-07 EF hand domain
EF-hand_1 PF00036 117 - 145 6.2e-08 EF hand domain
EF-hand_7 PF13499 117 - 179 1.6e-16 EF-hand domain pair
EF-hand_6 PF13405 117 - 146 4.2e-07 EF-hand domain
EF-hand_5 PF13202 121 - 141 3.4e-07 EF hand
EF-hand_8 PF13833 142 - 180 3.7e-09 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 3.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 469
AccBSI CCGCTC 1 cut(s) 479
AccI GTMKAC 2 cut(s) 483, 507
AciI CCGC 1 cut(s) 479
AclWI GGATC 1 cut(s) 534
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 385, 469
AgsI TTSAA 3 cut(s) 262, 301, 427
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 2 cut(s) 15, 265
AluI AGCT 2 cut(s) 15, 265
Alw21I GWGCWC 2 cut(s) 220, 321
Alw26I GTCTC 1 cut(s) 541
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 1 cut(s) 204
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 204
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 220, 321
BbvI GCAGC 1 cut(s) 459
BccI CCATC 5 cut(s) 170, 278, 383, 485, 491
BciT130I CCWGG 1 cut(s) 114
BcnI CCSGG 2 cut(s) 85, 86
BcoDI GTCTC 1 cut(s) 541
BfmI CTRYAG 1 cut(s) 199
BisI GCNGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 449
Bme1390I CCNGG 3 cut(s) 85, 86, 114
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 204
BmrFI CCNGG 3 cut(s) 85, 86, 114
BpuMI CCSGG 2 cut(s) 85, 86
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 469
Bse1I ACTGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 5 cut(s) 181, 289, 351, 394, 502
BseLI CCNNNNNNNGG 2 cut(s) 385, 469
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 321
BseXI GCAGC 1 cut(s) 459
BseYI CCCAGC 1 cut(s) 433
BsgI GTGCAG 2 cut(s) 423, 466
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 2 cut(s) 220, 321
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 385, 469
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 364, 464
BsnI GGCC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 220, 321
Bsp143I GATC 1 cut(s) 539
BspACI CCGC 1 cut(s) 479
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 202
BspHI TCATGA 1 cut(s) 522
BspPI GGATC 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 479
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 2 cut(s) 189, 511
BstF5I GGATG 5 cut(s) 181, 289, 351, 394, 502
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 1 cut(s) 539
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 3 cut(s) 83, 84, 112
BstSFI CTRYAG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 5 cut(s) 181, 289, 351, 394, 502
Cac8I GCNNGC 1 cut(s) 464
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 204
Cfr9I CCCGGG 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 112
Csp6I GTAC 1 cut(s) 31
CviAII CATG 6 cut(s) 10, 235, 310, 523, 529, 544
CviJI RGCY 7 cut(s) 15, 105, 124, 206, 265, 433, 450
CviKI_1 RGCY 7 cut(s) 15, 105, 124, 206, 265, 433, 450
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 2 cut(s) 189, 511
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco32I GATATC 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 112
EcoRV GATATC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 6 cut(s) 13, 238, 313, 526, 532, 547
FaiI YATR 9 cut(s) 11, 209, 236, 311, 416, 503, 524, 530, 545
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 101
FatI CATG 6 cut(s) 9, 234, 309, 522, 528, 543
FblI GTMKAC 2 cut(s) 483, 507
Fnu4HI GCNGC 1 cut(s) 448
FokI GGATG 5 cut(s) 188, 296, 338, 401, 509
Fsp4HI GCNGC 1 cut(s) 448
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 448
GsaI CCCAGC 1 cut(s) 437
HaeIII GGCC 1 cut(s) 206
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 60
Hin1II CATG 6 cut(s) 13, 238, 313, 526, 532, 547
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 147, 535
HpaII CCGG 1 cut(s) 85
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 3 cut(s) 319, 484, 508
Hpy188III TCNNGA 2 cut(s) 355, 523
Hpy8I GTNNAC 3 cut(s) 319, 484, 508
HpyCH4V TGCA 3 cut(s) 319, 404, 447
HpyF3I CTNAG 2 cut(s) 189, 511
Hsp92II CATG 6 cut(s) 13, 238, 313, 526, 532, 547
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 1 cut(s) 539
Lsp1109I GCAGC 1 cut(s) 459
MabI ACCWGGT 1 cut(s) 112
MalI GATC 1 cut(s) 541
MbiI CCGCTC 1 cut(s) 479
MboI GATC 1 cut(s) 539
MboII GAAGA 4 cut(s) 193, 419, 439, 467
MhlI GDGCHC 2 cut(s) 220, 321
MlyI GAGTC 2 cut(s) 156, 529
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 4 cut(s) 91, 423, 435, 461
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 2 cut(s) 107, 294
MslI CAYNNNNRTG 2 cut(s) 314, 527
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 3 cut(s) 85, 86, 114
Mva1269I GAATGC 2 cut(s) 364, 464
MvaI CCWGG 1 cut(s) 114
NciI CCSGG 2 cut(s) 85, 86
NdeII GATC 1 cut(s) 539
NlaIII CATG 6 cut(s) 13, 238, 313, 526, 532, 547
NsbI TGCGCA 1 cut(s) 59
PagI TCATGA 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PctI GAATGC 2 cut(s) 364, 464
PdmI GAANNNNTTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 1 cut(s) 449
PleI GAGTC 2 cut(s) 155, 529
PpsI GAGTC 2 cut(s) 155, 529
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 433
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 1 cut(s) 204
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 2 cut(s) 314, 527
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 107, 294
SatI GCNGC 1 cut(s) 448
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 2 cut(s) 156, 529
ScrFI CCNGG 3 cut(s) 85, 86, 114
SduI GDGCHC 2 cut(s) 220, 321
SetI ASST 7 cut(s) 17, 39, 115, 267, 334, 381, 446
SexAI ACCWGGT 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 199
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 314, 527
SsiI CCGC 1 cut(s) 479
StyD4I CCNGG 3 cut(s) 83, 84, 112
TaqI TCGA 6 cut(s) 5, 70, 120, 453, 507, 538
TatI WGTACW 1 cut(s) 30
Tru1I TTAA 2 cut(s) 107, 294
Tru9I TTAA 2 cut(s) 107, 294
TseI GCWGC 1 cut(s) 447
TspDTI ATGAA 3 cut(s) 155, 298, 511
TspGWI ACGGA 1 cut(s) 61
TspMI CCCGGG 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 469
VneI GTGCAC 1 cut(s) 317
XmaI CCCGGG 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 483, 507
XmnI GAANNNNTTC 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.