Rh6AG093600

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015472
Physical Location & Seq
Forward (+)
13881362 .. 13881919
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG093600.1

Sequence Viewer

Length: 558 bp
ATGTCGAACGTGAGCTTCCTTGATTTCCAGTATAACCTTTCAAAAAGGAAGTTTCTGCGCAAACCAACTCGATTGTTTTCCTCCCGGGACAGACAAAACTCTGGCTTAACACCTAGTTTCGAGCCAAATCTCAATGAGATGAAGCGGGTCTTTGAGAAATTTGACACCGACAAGGATGGGAAGATATCTCAGCAGGATTACAAGGCAATATTGAGAGCACTTGGAAAGGAAAGCATGGTTGGAGAAGTGCCAAAGATATTCAAGATTGTTGATTTGGACGGGGATGGGTTTATTAACTTCAAGGAGTTCATGGAAGTACACAAGAAGGCAGGTGGGGTGAGGACAATCGACATCCAGCATGCTTTCCAAACATTTGATCTGAATGGGGATGGAAAAATAAGTGCGGAAGAAGTTATGGAAGTGCTGAAGAGGCTGGGAGAGAGGAGCAGCCTCGAAGAATGCCAGCGAATGGTTAGAGCGGTAGACACCGATGGTGATGGTATGGTCGACATGGACGAGTTCATGACAATGATGACTCGATCCATGCGACGTACTTAG

Protein Analysis

185

Amino Acids

21.37

Weight (kDa)

6.76

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 45 - 106 6.2e-13 EF-hand domain pair
EF-hand_1 PF00036 46 - 74 2.3e-07 EF hand domain
EF-hand_6 PF13405 46 - 75 7.5e-08 EF-hand domain
EF-hand_1 PF00036 85 - 105 6.3e-07 EF hand domain
EF-hand_7 PF13499 117 - 179 3.3e-16 EF-hand domain pair
EF-hand_6 PF13405 117 - 146 3.6e-07 EF-hand domain
EF-hand_1 PF00036 118 - 145 1.9e-07 EF hand domain
EF-hand_5 PF13202 118 - 141 9.4e-08 EF hand
EF-hand_8 PF13833 143 - 180 2.9e-07 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 1.4e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc16I TGCGCA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 469
AccBSI CCGCTC 1 cut(s) 479
AccI GTMKAC 2 cut(s) 483, 507
AciI CCGC 3 cut(s) 145, 404, 479
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 2 cut(s) 318, 553
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 3 cut(s) 42, 262, 301
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 220
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 447
ApoI RAATTY 1 cut(s) 158
AspLEI GCGC 1 cut(s) 60
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 2 cut(s) 349, 506
AvaI CYCGRG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 459
BccI CCATC 5 cut(s) 170, 278, 383, 485, 491
BcnI CCSGG 2 cut(s) 85, 86
BfaI CTAG 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 449
Bme1390I CCNGG 2 cut(s) 85, 86
BmeT110I CYCGRG 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 85, 86
BpuMI CCSGG 2 cut(s) 85, 86
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse1I ACTGG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 4 cut(s) 181, 289, 351, 394
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 459
BseYI CCCAGC 1 cut(s) 433
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 376, 539
BspACI CCGC 3 cut(s) 145, 404, 479
BspCNI CTCAG 1 cut(s) 202
BspHI TCATGA 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 479
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 2 cut(s) 376, 539
Bst6I CTCTTC 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 360, 464
BstDEI CTNAG 2 cut(s) 189, 555
BstF5I GGATG 4 cut(s) 181, 289, 351, 394
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 2 cut(s) 379, 542
BstMBI GATC 2 cut(s) 376, 539
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 83, 84
BstV1I GCAGC 1 cut(s) 459
BtsCI GGATG 4 cut(s) 181, 289, 351, 394
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 2 cut(s) 360, 464
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 60
Cfr9I CCCGGG 1 cut(s) 84
Csp6I GTAC 2 cut(s) 317, 552
CviAII CATG 6 cut(s) 235, 310, 359, 511, 523, 544
CviJI RGCY 5 cut(s) 15, 105, 124, 433, 450
CviKI_1 RGCY 5 cut(s) 15, 105, 124, 433, 450
CviQI GTAC 2 cut(s) 317, 552
DdeI CTNAG 2 cut(s) 189, 555
DpnI GATC 2 cut(s) 378, 541
DpnII GATC 2 cut(s) 376, 539
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco32I GATATC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 186
FaeI CATG 6 cut(s) 238, 313, 362, 514, 526, 547
FaiI YATR 9 cut(s) 33, 236, 311, 360, 416, 503, 512, 524, 545
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 101
FatI CATG 6 cut(s) 234, 309, 358, 510, 522, 543
FauI CCCGC 1 cut(s) 138
FblI GTMKAC 2 cut(s) 483, 507
Fnu4HI GCNGC 1 cut(s) 448
FokI GGATG 4 cut(s) 188, 296, 338, 401
Fsp4HI GCNGC 1 cut(s) 448
FspBI CTAG 1 cut(s) 114
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 448
GsaI CCCAGC 1 cut(s) 437
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 60
Hin1II CATG 6 cut(s) 238, 313, 362, 514, 526, 547
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 1 cut(s) 535
HpaII CCGG 1 cut(s) 85
HphI GGTGA 2 cut(s) 349, 506
Hpy166II GTNNAC 3 cut(s) 319, 484, 508
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 262, 523
Hpy8I GTNNAC 3 cut(s) 319, 484, 508
Hpy99I CGWCG 1 cut(s) 552
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 9, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 2 cut(s) 189, 555
HpySE526I ACGT 2 cut(s) 9, 550
Hsp92II CATG 6 cut(s) 238, 313, 362, 514, 526, 547
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 2 cut(s) 376, 539
LmnI GCTCC 1 cut(s) 444
LpnPI CCDG 8 cut(s) 41, 87, 98, 179, 315, 368, 419, 476
Lsp1109I GCAGC 1 cut(s) 459
MaeI CTAG 1 cut(s) 114
MaeII ACGT 2 cut(s) 9, 550
MalI GATC 2 cut(s) 378, 541
MbiI CCGCTC 1 cut(s) 479
MboI GATC 2 cut(s) 376, 539
MboII GAAGA 4 cut(s) 193, 419, 439, 467
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 1 cut(s) 158
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 5 cut(s) 91, 333, 423, 435, 461
MseI TTAA 2 cut(s) 107, 294
MslI CAYNNNNRTG 1 cut(s) 527
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 85, 86
Mva1269I GAATGC 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 430
NciI CCSGG 2 cut(s) 85, 86
NdeII GATC 2 cut(s) 376, 539
NlaIII CATG 6 cut(s) 238, 313, 362, 514, 526, 547
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 1 cut(s) 362
PaeI GCATGC 1 cut(s) 362
PagI TCATGA 1 cut(s) 522
PaqCI CACCTGC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 544
PctI GAATGC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 1 cut(s) 449
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 433
RsaI GTAC 2 cut(s) 318, 553
RsaNI GTAC 2 cut(s) 317, 552
RseI CAYNNNNRTG 1 cut(s) 527
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 107, 294
SatI GCNGC 1 cut(s) 448
Sau3AI GATC 2 cut(s) 376, 539
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 85, 86
SduI GDGCHC 1 cut(s) 220
SetI ASST 6 cut(s) 12, 17, 39, 115, 334, 553
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 527
SphI GCATGC 1 cut(s) 362
Sse9I AATT 1 cut(s) 158
SsiI CCGC 3 cut(s) 145, 404, 479
SspI AATATT 1 cut(s) 210
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 83, 84
TaiI ACGT 2 cut(s) 12, 553
TaqI TCGA 7 cut(s) 5, 70, 120, 348, 453, 507, 538
TasI AATT 1 cut(s) 158
TatI WGTACW 1 cut(s) 316
Tru1I TTAA 2 cut(s) 107, 294
Tru9I TTAA 2 cut(s) 107, 294
TseI GCWGC 1 cut(s) 447
TspDTI ATGAA 3 cut(s) 155, 298, 511
TspMI CCCGGG 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 469
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 362
XmaI CCCGGG 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 483, 507
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.