Rh6BG084400

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015472
Physical Location & Seq
Forward (+)
13173120 .. 13173677
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG084400.1

Sequence Viewer

Length: 558 bp
ATGTCGAACGTGAGCTTCCTTGATTTCCAGTATAACCTTTCAAAAAGGAAGTTTCTGCGCAAACCAACTCGATTGTTTTCCTCCCGGGACAGACAAAACTCTGGCTTAACACCTAGTTTCGAGCCAAATCTCAATGAGATGAAGCGGGTCTTTGATAAATTTGACACCAACAAGGATGGGAAGATATCTCAGCAGGATTACAAGGCAATATTGAGAGCACTTGGAAAGGAAAGCATGGTTGGAGAAGTGCCAAAGATATTCAAGATTGTTGATTTGGACGGGGATGGGTTTATTAACTTCAAGGAGTTCATGGAAGTGCACAAGAAGGCAGGTGGGGTGAGGACAATCGACATCCAGAATGCTTTCCAAACATTTGATCTGAATGGAGATGGAAAAATAAGTGCGGAAGAAGTTATGGAAGTGCTGAAGAGGCTGGGAGAGAGGAACAGCCTCGAAGAATGCCAGCGAATGGTTAGAGCGGTAGACACCGATGGTGATGGTATGGTCGACATGGACGAGTTCATGACTATGATGACTCGATCCATGCAACGTACTTAG

Protein Analysis

185

Amino Acids

21.34

Weight (kDa)

6.63

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 45 - 106 3.6e-13 EF-hand domain pair
EF-hand_1 PF00036 46 - 74 4.5e-07 EF hand domain
EF-hand_6 PF13405 46 - 75 6.7e-08 EF-hand domain
EF-hand_1 PF00036 85 - 105 6.3e-07 EF hand domain
EF-hand_1 PF00036 117 - 145 6.2e-08 EF hand domain
EF-hand_7 PF13499 117 - 179 4.1e-16 EF-hand domain pair
EF-hand_6 PF13405 117 - 146 4.2e-07 EF-hand domain
EF-hand_5 PF13202 121 - 141 3.4e-07 EF hand
EF-hand_8 PF13833 143 - 180 4.1e-07 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 1.4e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc16I TGCGCA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 469
AccBSI CCGCTC 1 cut(s) 479
AccI GTMKAC 2 cut(s) 483, 507
AciI CCGC 3 cut(s) 145, 404, 479
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 3 cut(s) 42, 262, 301
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 2 cut(s) 220, 321
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 84
ApaLI GTGCAC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 60
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 2 cut(s) 349, 506
AvaI CYCGRG 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 220, 321
BccI CCATC 5 cut(s) 170, 278, 383, 485, 491
BcnI CCSGG 2 cut(s) 85, 86
BfaI CTAG 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 320
Bme1390I CCNGG 2 cut(s) 85, 86
BmeT110I CYCGRG 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 85, 86
BpuMI CCSGG 2 cut(s) 85, 86
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse1I ACTGG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 3 cut(s) 181, 289, 351
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 321
BseYI CCCAGC 1 cut(s) 433
BsiHKAI GWGCWC 2 cut(s) 220, 321
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 364, 464
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 220, 321
Bsp143I GATC 2 cut(s) 376, 539
BspACI CCGC 3 cut(s) 145, 404, 479
BspCNI CTCAG 1 cut(s) 202
BspHI TCATGA 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 479
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 2 cut(s) 376, 539
Bst6I CTCTTC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 2 cut(s) 189, 555
BstF5I GGATG 3 cut(s) 181, 289, 351
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 2 cut(s) 379, 542
BstMBI GATC 2 cut(s) 376, 539
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 83, 84
BstSLI GKGCMC 1 cut(s) 321
BtsCI GGATG 3 cut(s) 181, 289, 351
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 1 cut(s) 464
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 60
Cfr9I CCCGGG 1 cut(s) 84
Csp6I GTAC 1 cut(s) 552
CviAII CATG 5 cut(s) 235, 310, 511, 523, 544
CviJI RGCY 5 cut(s) 15, 105, 124, 433, 450
CviKI_1 RGCY 5 cut(s) 15, 105, 124, 433, 450
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 2 cut(s) 189, 555
DpnI GATC 2 cut(s) 378, 541
DpnII GATC 2 cut(s) 376, 539
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco32I GATATC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 186
FaeI CATG 5 cut(s) 238, 313, 514, 526, 547
FaiI YATR 9 cut(s) 33, 236, 311, 416, 503, 512, 524, 530, 545
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 101
FatI CATG 5 cut(s) 234, 309, 510, 522, 543
FauI CCCGC 1 cut(s) 138
FblI GTMKAC 2 cut(s) 483, 507
FokI GGATG 3 cut(s) 188, 296, 338
FspBI CTAG 1 cut(s) 114
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GsaI CCCAGC 1 cut(s) 437
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 60
Hin1II CATG 5 cut(s) 238, 313, 514, 526, 547
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 1 cut(s) 535
HpaII CCGG 1 cut(s) 85
HphI GGTGA 2 cut(s) 349, 506
Hpy166II GTNNAC 3 cut(s) 319, 484, 508
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 3 cut(s) 262, 355, 523
Hpy8I GTNNAC 3 cut(s) 319, 484, 508
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 9, 550
HpyCH4V TGCA 2 cut(s) 319, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 2 cut(s) 189, 555
HpySE526I ACGT 2 cut(s) 9, 550
Hsp92II CATG 5 cut(s) 238, 313, 514, 526, 547
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 2 cut(s) 376, 539
LpnPI CCDG 8 cut(s) 41, 87, 98, 179, 315, 368, 419, 476
MaeI CTAG 1 cut(s) 114
MaeII ACGT 2 cut(s) 9, 550
MalI GATC 2 cut(s) 378, 541
MbiI CCGCTC 1 cut(s) 479
MboI GATC 2 cut(s) 376, 539
MboII GAAGA 4 cut(s) 193, 419, 439, 467
MhlI GDGCHC 2 cut(s) 220, 321
MluCI AATT 1 cut(s) 158
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 5 cut(s) 91, 333, 423, 435, 461
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 2 cut(s) 107, 294
MslI CAYNNNNRTG 2 cut(s) 314, 527
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 85, 86
Mva1269I GAATGC 2 cut(s) 364, 464
MwoI GCNNNNNNNGC 1 cut(s) 430
NciI CCSGG 2 cut(s) 85, 86
NdeII GATC 2 cut(s) 376, 539
NlaIII CATG 5 cut(s) 238, 313, 514, 526, 547
NsbI TGCGCA 1 cut(s) 59
PagI TCATGA 1 cut(s) 522
PaqCI CACCTGC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PctI GAATGC 2 cut(s) 364, 464
PdmI GAANNNNTTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 469
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 433
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 2 cut(s) 314, 527
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 107, 294
Sau3AI GATC 2 cut(s) 376, 539
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 85, 86
SduI GDGCHC 2 cut(s) 220, 321
SetI ASST 6 cut(s) 12, 17, 39, 115, 334, 553
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 314, 527
Sse9I AATT 1 cut(s) 158
SsiI CCGC 3 cut(s) 145, 404, 479
SspI AATATT 1 cut(s) 210
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 83, 84
TaiI ACGT 2 cut(s) 12, 553
TaqI TCGA 7 cut(s) 5, 70, 120, 348, 453, 507, 538
TasI AATT 1 cut(s) 158
Tru1I TTAA 2 cut(s) 107, 294
Tru9I TTAA 2 cut(s) 107, 294
TspDTI ATGAA 3 cut(s) 155, 298, 511
TspMI CCCGGG 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 469
VneI GTGCAC 1 cut(s) 317
XapI RAATTY 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 483, 507
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.