MD10G1094100.v1.1

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015472
Physical Location & Seq
Reverse (-)
14668494 .. 14669339
846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1094100.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGATATGTCGAATGTCAACTCCCTCGACTTCCAGTACAGCCTCTCAAAGCGGAAGTTTCTGTGCAAACCATCTCGGATATTTTCCTTCCAAGACAGGAAAAACTCCGGCTTAGCACTTACTTTTGAGCCAAATCACAATGAGATGAAGAAAGTCTTTGACAAGTTCGACTCCAACAAAGACGGGAAGATATCTCCGGCGGAGTACAAGGCCATATTGAGAGCACTGGGAAAGGGAAGTAAGGTTGGAGAAGTGCAGAAGATTTTCAAGATTGTTGATCTGGATCGGGATGGCTTTATCAATTTCAAGGAGTTCTTGGATGTACACAACGGAGGGATTCGGACGATAGACATCCAGAACGCTTTTCAGATGTTTGATTTGAATGGCGACGGAAAAATAAGCGCAGAAGAAGTTATGGAAGTGCTAACGGGATTGGAGGAGAGTTGCAACCTTGACGACTGCCGGCGGATAGTGAGAGCGGTAGACACTGATGGGGATGGTATGGTTGACTTAAATGATTTCATGACCATGATGACTAGTTCCATGAGACGTGCATAG

Protein Analysis

186

Amino Acids

21.02

Weight (kDa)

5.5

Isoelectric Point (pI)

27.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 48 - 107 2.1e-14 EF-hand domain pair
EF-hand_1 PF00036 48 - 76 2.2e-07 EF hand domain
EF-hand_6 PF13405 48 - 77 1.8e-07 EF-hand domain
EF-hand_5 PF13202 50 - 72 8.1e-07 EF hand
EF-hand_8 PF13833 60 - 108 1.1e-09 EF-hand domain pair
EF-hand_1 PF00036 84 - 107 7.1e-07 EF hand domain
EF-hand_7 PF13499 117 - 179 1.8e-16 EF-hand domain pair
EF-hand_1 PF00036 117 - 144 7.1e-08 EF hand domain
EF-hand_6 PF13405 117 - 144 5.4e-07 EF-hand domain
EF-hand_5 PF13202 121 - 141 1.1e-07 EF hand
EF-hand_8 PF13833 145 - 179 5.4e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15680
fragaria_vesca FvH4_2g06140
malus_domestica MD05G1080100.v1.1 MD10G1094100.v1.1
prunus_persica Prupe.8G123900_v2.0.a1
pyrus_communis pycom05g07320
rosa_chinensis RchiOBHm_Chr6g0257051
rosa_laevigata RLG00000014673
rosa_multiflora Rmu_sc0000659.1_g000010
rosa_roxburghii Rroxscaffold_7G00208460
rosa_rugosa Rorug05G0575600
rosa_samantha Rh6AG093600 Rh6BG084400 Rh6CG081100 Rh6DG076500
rosa_wichuraiana Rw6G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 483
AciI CCGC 4 cut(s) 52, 200, 466, 479
AclWI GGATC 1 cut(s) 291
AfaI GTAC 3 cut(s) 38, 206, 324
AgsI TTSAA 3 cut(s) 268, 307, 382
AhlI ACTAGT 1 cut(s) 536
AjiI CACGTC 1 cut(s) 551
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 1 cut(s) 291
AoxI GGCC 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 226
BccI CCATC 4 cut(s) 79, 284, 485, 491
BcoDI GTCTC 1 cut(s) 541
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 1 cut(s) 537
BlpI GCTNAGC 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 551
BmrI ACTGGG 1 cut(s) 236
BmuI ACTGGG 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 112
BsaBI GATNNNNATC 2 cut(s) 282, 350
Bse118I RCCGGY 1 cut(s) 462
Bse1I ACTGG 2 cut(s) 34, 231
Bse8I GATNNNNATC 2 cut(s) 282, 350
BseGI GGATG 4 cut(s) 295, 325, 351, 502
BseJI GATNNNNATC 2 cut(s) 282, 350
BseNI ACTGG 2 cut(s) 34, 231
BseRI GAGGAG 1 cut(s) 452
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 3 cut(s) 108, 197, 463
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 226
Bsp1407I TGTACA 1 cut(s) 322
Bsp143I GATC 2 cut(s) 277, 283
Bsp1720I GCTNAGC 1 cut(s) 112
BspACI CCGC 4 cut(s) 52, 200, 466, 479
BspANI GGCC 1 cut(s) 212
BspHI TCATGA 1 cut(s) 522
BspPI GGATC 1 cut(s) 291
BsrBI CCGCTC 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 322
BsrI ACTGG 2 cut(s) 34, 231
BssAI RCCGGY 1 cut(s) 462
BssMI GATC 2 cut(s) 277, 283
BstAUI TGTACA 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 4 cut(s) 295, 325, 351, 502
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 2 cut(s) 280, 286
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 2 cut(s) 277, 283
BsuRI GGCC 1 cut(s) 212
BtrI CACGTC 1 cut(s) 551
BtsCI GGATG 4 cut(s) 295, 325, 351, 502
BtsIMutI CAGTG 2 cut(s) 224, 486
Cac8I GCNNGC 1 cut(s) 464
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 462
Csp6I GTAC 3 cut(s) 37, 205, 323
CviAII CATG 3 cut(s) 523, 529, 544
CviJI RGCY 5 cut(s) 42, 111, 130, 212, 294
CviKI_1 RGCY 5 cut(s) 42, 111, 130, 212, 294
CviQI GTAC 3 cut(s) 37, 205, 323
DdeI CTNAG 1 cut(s) 112
DpnI GATC 2 cut(s) 279, 285
DpnII GATC 2 cut(s) 277, 283
EciI GGCGGA 2 cut(s) 215, 481
Eco32I GATATC 1 cut(s) 192
EcoRV GATATC 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 3 cut(s) 526, 532, 547
FaiI YATR 8 cut(s) 8, 215, 416, 503, 524, 530, 545, 556
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 299, 331
FatI CATG 3 cut(s) 522, 528, 543
FblI GTMKAC 1 cut(s) 483
FokI GGATG 4 cut(s) 302, 332, 338, 509
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 403
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 108, 197, 463
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 526, 532, 547
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 2 cut(s) 19, 508
HindII GTYRAC 2 cut(s) 19, 508
HinfI GANTC 2 cut(s) 170, 337
HpaII CCGG 3 cut(s) 108, 197, 463
Hpy166II GTNNAC 4 cut(s) 19, 325, 484, 508
Hpy188I TCNGA 3 cut(s) 78, 342, 369
Hpy188III TCNNGA 5 cut(s) 268, 281, 287, 355, 523
Hpy8I GTNNAC 4 cut(s) 19, 325, 484, 508
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 4 cut(s) 66, 256, 447, 554
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 3 cut(s) 526, 532, 547
HspAI GCGC 1 cut(s) 402
KroI GCCGGC 1 cut(s) 462
KroNI GCCGGC 1 cut(s) 464
Kzo9I GATC 2 cut(s) 277, 283
LpnPI CCDG 8 cut(s) 47, 82, 121, 210, 212, 266, 368, 476
MaeI CTAG 1 cut(s) 537
MaeII ACGT 1 cut(s) 550
MalI GATC 2 cut(s) 279, 285
MbiI CCGCTC 1 cut(s) 479
MboI GATC 2 cut(s) 277, 283
MboII GAAGA 4 cut(s) 160, 199, 271, 419
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 1 cut(s) 301
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 2 cut(s) 198, 226
MnlI CCTC 4 cut(s) 35, 53, 326, 430
MroNI GCCGGC 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 1 cut(s) 512
MslI CAYNNNNRTG 1 cut(s) 527
MspI CCGG 3 cut(s) 108, 197, 463
NaeI GCCGGC 1 cut(s) 464
NdeII GATC 2 cut(s) 277, 283
NgoMIV GCCGGC 1 cut(s) 462
NlaIII CATG 3 cut(s) 526, 532, 547
PagI TCATGA 1 cut(s) 522
PdiI GCCGGC 1 cut(s) 464
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 337
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
RsaI GTAC 3 cut(s) 38, 206, 324
RsaNI GTAC 3 cut(s) 37, 205, 323
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 1 cut(s) 512
Sau3AI GATC 2 cut(s) 277, 283
SchI GAGTC 1 cut(s) 164
SduI GDGCHC 1 cut(s) 226
SetI ASST 3 cut(s) 246, 453, 553
SmiMI CAYNNNNRTG 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 536
Sse9I AATT 1 cut(s) 301
SsiI CCGC 4 cut(s) 52, 200, 466, 479
SspMI CTAG 1 cut(s) 537
TaiI ACGT 1 cut(s) 553
TaqI TCGA 3 cut(s) 11, 27, 168
TasI AATT 1 cut(s) 301
TatI WGTACW 3 cut(s) 36, 204, 322
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 1 cut(s) 512
Tru9I TTAA 1 cut(s) 512
TscAI CASTG 2 cut(s) 231, 493
TspDTI ATGAA 2 cut(s) 161, 511
TspGWI ACGGA 2 cut(s) 345, 405
TspRI CASTG 2 cut(s) 231, 493
XmiI GTMKAC 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.