Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009254

Physical Location & Seq

Genomic Coordinates Reverse (-)

9504659 .. 9508895

Gene Structure 4237 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G22400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGGGAAGAGGAGGAAGACATAGAGGTCGTACTCAGAGAAAAGATTTCAAAGAAAGCAGAGAGAATGTTTGGAAACGACCCAAATCCGATGCTTCCGTTGATGGTTCCGACAACGCCGTACCGGAACAAAAACCCACTTGGGAACCTATTGTCACTGTTAACCCTAACTTCGAGGAATATTACAAGAAACAAGGGATAGTGAAAGCTGAAGAGTGGGATTTGTTCATGGAGATTCTTCGTAAGCCTTTACCTGCTGCGTTTAGGGTTAACTCCAATGGCCAATTTTGCGATGAGATTATATCGATCTTGGAGAATGACTTTATGAAATCACTTCAGGCTGAGGCCATAGAAGGTGGTGAATTGGAGGCTATTAAGCCCTTGCCTTGGTATCCAAAGAATCTCGCTTGGCATTCTAATTTTTCTCGAAAGGAGATAAGAAAAAATCAGACACTTGAGAGGTTTCATGAGTTTCTGAAGTTAGAAAATGAAGTTGGAAATATTACTAGGCAGGAAGCTGTTAGCATGGTACCTCCTCTCTTCCTAGACGTACATCCAGATCATTTCGTACTTGACATGTGTGCTGCACCGGGTTCCAAAACATTTCAGCTGCTTGAGATTATACATGAAGCATCAGAACCAGGATCTCTTCCTAATGGATTGGTGGTGGCTAATGATGTTGATTTTAAAAGATCTAACCTTCTCATTCACCAAACAAAGAGAATGTGCACATCCAACTTGATAGTGACAAATCATGAAGGGCAACAGTTTCCTGGTTGCCGTTTGAACAAATCCCGAGCTTCTGAGAAAGGAATAAGTGAAAATATGCCTATTAATCAACTTGCCTTTGACCGTGTTCTATGCGATGTTCCGTGCAGTGGTGATGGTACACTGCGCAAGGCTCCAGATATCTGGCGCAAATGGAACTCTGGTATGGGCAATGGACTTCATAGCCTTCAGATTATTCTTGCTATGAGAGGTTTATCTCTGTTGAAAGTTGGTGGGAAGATGATATACTCAACCTGCTCAATGAACCCAGTGGAGGATGAAGCTGTTGTTGCTGAGATTCTAAGGAGGTGTGGAGACTCTGTTGAACTTTTAGATGTTTCCGATAAGCTTCCTGAACTTATACGAAGACCAGGACTTAAGGCATGGAAGGTGCGTGATAAAGGTGGGTGGTTTACTTCTTACAAAGATGTTCCACAAAACCGGAGAGGTGGAGTTCTTGTGAGCATGTTTCCTTCTGGGAAATACCTCAAGGACTCAACTGAAACCACGGAAAAGAACGAGAATGGTGATGTTAATGGCTGTGAGGATGGACTCAAAGAAACAGATATTTCTGTGGTGGATGCTACTCCTGAAGAACAAGCTGAGGAAGTCTCTGATCTTCCACTTGAACGTTGCATGAGGATAATACCTCATGATCAGAACACCGGAGCCTTCTTCATTGCGGTCCTTCAAAAAAAATCTCCCTTACCAGAATTTCAGGAGAAACCAAATACGAAAAGGAACTCAACTGCTAAGTCTACTGACTCGACAGAAAAGTCTCCGAGTAAAGAATCTGTTGTTACAGTGGATGCTGGTGTACCAGATGAGAGTGCAGTGGAGAAAGTTATTGAAGCAGATTCAAACATTGAGAAAAATGATAGCTTGGAGCCTGAGAAGAAAATCACAGAAGGAGAAAGCATCACGGAAGATAAAGAGGCCAATTCGAGTAATGCGGGAGGCAAGAGAAAAGTACCGATGCAAGGGAAGTGGAAAGGCTTTGACCCAGTTGTTTTCGTGAAAGATGAAACAGTAATCAATGGCATCAAGGAATTCTACGGTATCAAAGATGAATCATTTCCATTACATGGTCATCTCGTGGCAAGAAACACCGACACAAGCAGCGTTAAGAGGATTTACTATGTTTCAAAATCAGTTAAGGAAGTTCTTCAGTTGAATTTTGCAGTCGGGCAGCAGCTTAAGATCGCTTCTGTTGGCCTCAAAATGTTTGAGAGACAATCGGCAAAAGAAGGTTCAAGCACGCTGTGCCCATTCCGTATATCATCCGAGGGACTACCTGTGATTCTTCCATACATTACCAAACAAGTACTTTATACTCCAATGGCAGACTTCAAACTTCTCCTGCAAGACAAATCAATCAAGTTTCACGATTTTGTCAATCCACAGTTGGCCCAGAAAGCAACTGACCTTGTTATGGGAAGCTGCGTGGTGATTCTCAGCGATGGTGAAGTACCGGTGAAAGCGGATGCATCAACAATTGCCATCAGTTGCTGGAGAGGGAAGGCTAGTTTGGCTGTTATGGCCACTGTCGCAGATTGCCAGGAGCTGCTAGAGAGATTTGCCGAGAAAACACCAAAAACAGAAGGTGGTTCGGTAAATGGAAGCAACGGCGATTCGGACGGTCCACTAGCTATGGAGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016427 GO:0016428 GO:0016740 GO:0016741 GO:0030488 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltr_RsmB-F

Protein Analysis

808

Amino Acids

89.81

Weight (kDa)

5.77

Isoelectric Point (pI)

40.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltr_RsmB-F	PF01189	180 - 366	1.1e-32	16S rRNA methyltransferase RsmB/F
Pre-PUA_NSUN2	PF25376	590 - 694	8.8e-34	NSUN2 Pre-PUA domain
PUA_NSUN2	PF25378	696 - 781	1e-22	NSUN2 PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22400 AT4G40000 AT4G40000
fragaria_vesca	FvH4_1g20370 FvH4_1g20370 FvH4_1g20370
malus_domestica	MD01G1034300.v1.1 MD15G1321600.v1.1
prunus_persica	Prupe.6G191700_v2.0.a1 Prupe.6G191700_v2.0.a1
pyrus_communis	pycom01g06140 pycom15g28220
rosa_chinensis	RchiOBHm_Chr2g0112501
rosa_laevigata	RLG00000017986
rosa_multiflora	Rmu_sc0001499.1_g000061
rosa_roxburghii	Rroxscaffold_2G00131370
rosa_rugosa	Rorug02G0181300
rosa_samantha	Rh2AG234500 Rh2BG248100 Rh2CG239100 Rh2DG242100
rosa_wichuraiana	Rw2G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	24, 1540
Acc16I	TGCGCA	1 cut(s)	893
Acc36I	ACCTGC	2 cut(s)	259, 1028
Acc65I	GGTACC	1 cut(s)	526
AccB1I	GGYRCC	1 cut(s)	526
AccB7I	CCANNNNNTGG	1 cut(s)	1850
AccI	GTMKAC	1 cut(s)	1523
AciI	CCGC	3 cut(s)	1448, 1718, 2246
AclI	AACGTT	1 cut(s)	1396
AclWI	GGATC	1 cut(s)	649
AcoI	YGGCCR	2 cut(s)	277, 2304
AcsI	RAATTY	3 cut(s)	1478, 1814, 1939
AcuI	CTGAAG	6 cut(s)	228, 317, 494, 938, 1377, 1916
AdeI	CACNNNGTG	1 cut(s)	2028
AfiI	CCNNNNNNNGG	6 cut(s)	384, 875, 1039, 1745, 1850, 2197
AflII	CTTAAG	2 cut(s)	1142, 1961
AflIII	ACRYGT	1 cut(s)	573
AgeI	ACCGGT	1 cut(s)	2236
AjnI	CCWGG	4 cut(s)	637, 769, 1135, 2322
AjuI	GAANNNNNNNTTGG	4 cut(s)	474, 506, 2276, 2308
Alw21I	GWGCWC	1 cut(s)	728
Alw26I	GTCTC	5 cut(s)	1074, 1381, 1548, 1990, 2414
Alw44I	GTGCAC	1 cut(s)	724
AlwI	GGATC	1 cut(s)	649
AlwNI	CAGNNNCTG	2 cut(s)	2274, 2329
Ama87I	CYCGRG	1 cut(s)	792
AoxI	GGCC	6 cut(s)	277, 342, 1701, 1978, 2172, 2304
ApaLI	GTGCAC	1 cut(s)	724
ApeKI	GCWGC	8 cut(s)	254, 581, 607, 1884, 1954, 1957, 2205, 2329
ApoI	RAATTY	3 cut(s)	1478, 1814, 1939
AseI	ATTAAT	1 cut(s)	831
AsiGI	ACCGGT	1 cut(s)	2236
Asp700I	GAANNNNTTC	2 cut(s)	1839, 1929
Asp718I	GGTACC	1 cut(s)	526
AspLEI	GCGC	2 cut(s)	894, 915
AspS9I	GGNCC	3 cut(s)	1450, 2173, 2405
AsuC2I	CCSGG	1 cut(s)	588
AsuHPI	GGTGA	7 cut(s)	368, 698, 890, 1304, 2224, 2240, 2251
AvaI	CYCGRG	1 cut(s)	792
AvaII	GGWCC	2 cut(s)	1450, 2405
BaeGI	GKGCMC	2 cut(s)	728, 2033
BalI	TGGCCA	2 cut(s)	279, 2306
BanI	GGYRCC	1 cut(s)	526
BarI	GAAGNNNNNNTAC	2 cut(s)	995, 1027
BauI	CACGAG	1 cut(s)	1859
BbsI	GAAGAC	2 cut(s)	22, 1138
Bbv12I	GWGCWC	1 cut(s)	728
BbvCI	CCTCAGC	2 cut(s)	339, 1368
BbvI	GCAGC	8 cut(s)	241, 568, 594, 1896, 1966, 1969, 2192, 2316
BccI	CCATC	5 cut(s)	95, 875, 1307, 2219, 2273
BceAI	ACGGC	3 cut(s)	101, 762, 2407
BciT130I	CCWGG	4 cut(s)	639, 771, 1137, 2324
BciVI	GTATCC	1 cut(s)	399
BclI	TGATCA	1 cut(s)	1420
BcnI	CCSGG	1 cut(s)	588
BcoDI	GTCTC	5 cut(s)	1074, 1381, 1548, 1990, 2414
BfaI	CTAG	5 cut(s)	504, 542, 2289, 2333, 2411
BfrI	CTTAAG	2 cut(s)	1142, 1961
BfuAI	ACCTGC	2 cut(s)	259, 1028
BfuI	GTATCC	1 cut(s)	399
BglII	AGATCT	1 cut(s)	689
BisI	GCNGC	8 cut(s)	255, 582, 608, 1885, 1955, 1958, 2206, 2330
BlsI	GCNGC	8 cut(s)	256, 583, 609, 1886, 1956, 1959, 2207, 2331
BmcAI	AGTACT	1 cut(s)	2091
Bme1390I	CCNGG	5 cut(s)	588, 639, 771, 1137, 2324
Bme18I	GGWCC	2 cut(s)	1450, 2405
BmeT110I	CYCGRG	1 cut(s)	792
BmgT120I	GGNCC	3 cut(s)	1450, 2173, 2405
BmiI	GGNNCC	7 cut(s)	106, 144, 528, 592, 900, 1435, 1653
BmrFI	CCNGG	5 cut(s)	588, 639, 771, 1137, 2324
BmrI	ACTGGG	2 cut(s)	1028, 1763
BmsI	GCATC	9 cut(s)	79, 638, 1336, 1564, 1692, 1731, 1815, 2239, 2261
BmuI	ACTGGG	2 cut(s)	1028, 1763
BpiI	GAAGAC	2 cut(s)	22, 1138
BplI	GAGNNNNNCTC	2 cut(s)	1361, 1393
BpmI	CTGGAG	2 cut(s)	885, 2296
Bpu10I	CCTNAGC	2 cut(s)	339, 1368
BpuEI	CTTGAG	3 cut(s)	473, 632, 1238
BpuMI	CCSGG	1 cut(s)	588
Bsa29I	ATCGAT	1 cut(s)	302
BsaJI	CCNNGG	3 cut(s)	383, 1272, 2049
BsaWI	WCCGGW	4 cut(s)	121, 1206, 1430, 2236
BsaXI	ACNNNNNCTCC	4 cut(s)	1071, 1101, 1209, 1239
Bsc4I	CCNNNNNNNGG	6 cut(s)	384, 875, 1039, 1745, 1850, 2197
Bse118I	RCCGGY	1 cut(s)	2236
Bse1I	ACTGG	2 cut(s)	1034, 1769
Bse3DI	GCAATG	2 cut(s)	943, 1443
BseBI	CCWGG	4 cut(s)	639, 771, 1137, 2324
BseCI	ATCGAT	1 cut(s)	302
BseDI	CCNNGG	3 cut(s)	383, 1272, 2049
BseGI	GGATG	8 cut(s)	550, 728, 1048, 1318, 1351, 1579, 2045, 2254
BseLI	CCNNNNNNNGG	6 cut(s)	384, 875, 1039, 1745, 1850, 2197
BseMI	GCAATG	2 cut(s)	943, 1443
BseMII	CTCAG	7 cut(s)	47, 330, 792, 1050, 1359, 1647, 2233
BseNI	ACTGG	2 cut(s)	1034, 1769
BseRI	GAGGAG	2 cut(s)	24, 522
BseSI	GKGCMC	2 cut(s)	728, 2033
BseXI	GCAGC	8 cut(s)	241, 568, 594, 1896, 1966, 1969, 2192, 2316
BsgI	GTGCAG	3 cut(s)	567, 892, 1617
BshFI	GGCC	6 cut(s)	279, 344, 1703, 1980, 2174, 2306
BshNI	GGYRCC	1 cut(s)	526
BshTI	ACCGGT	1 cut(s)	2236
BshVI	ATCGAT	1 cut(s)	302
BsiHKAI	GWGCWC	1 cut(s)	728
BsiHKCI	CYCGRG	1 cut(s)	792
BsiSI	CCGG	5 cut(s)	122, 587, 1207, 1431, 2237
BslFI	GGGAC	1 cut(s)	2067
BslI	CCNNNNNNNGG	6 cut(s)	384, 875, 1039, 1745, 1850, 2197
BsmAI	GTCTC	5 cut(s)	1074, 1381, 1548, 1990, 2414
BsmFI	GGGAC	1 cut(s)	2067
BsmI	GAATGC	1 cut(s)	409
BsnI	GGCC	6 cut(s)	279, 344, 1703, 1980, 2174, 2306
BsoBI	CYCGRG	1 cut(s)	792
Bsp1286I	GDGCHC	2 cut(s)	728, 2033
Bsp143I	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
BspACI	CCGC	3 cut(s)	1448, 1718, 2246
BspANI	GGCC	6 cut(s)	279, 344, 1703, 1980, 2174, 2306
BspCNI	CTCAG	7 cut(s)	46, 331, 793, 1051, 1360, 1648, 2232
BspDI	ATCGAT	1 cut(s)	302
BspHI	TCATGA	3 cut(s)	463, 751, 1417
BspLI	GGNNCC	7 cut(s)	106, 144, 528, 592, 900, 1435, 1653
BspMI	ACCTGC	2 cut(s)	259, 1028
BspPI	GGATC	1 cut(s)	649
BspT107I	GGYRCC	1 cut(s)	526
BspTI	CTTAAG	2 cut(s)	1142, 1961
BsrDI	GCAATG	2 cut(s)	943, 1443
BsrFI	RCCGGY	1 cut(s)	2236
BsrI	ACTGG	2 cut(s)	1034, 1769
BssAI	RCCGGY	1 cut(s)	2236
BssECI	CCNNGG	3 cut(s)	383, 1272, 2049
BssMI	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
BssSI	CACGAG	1 cut(s)	1859
BssT1I	CCWWGG	1 cut(s)	383
Bst2BI	CACGAG	1 cut(s)	1859
Bst2UI	CCWGG	4 cut(s)	639, 771, 1137, 2324
Bst4CI	ACNGT	9 cut(s)	157, 765, 851, 1570, 1795, 1823, 2169, 2311, 2405
Bst6I	CTCTTC	3 cut(s)	204, 542, 651
BstAFI	CTTAAG	2 cut(s)	1142, 1961
BstAPI	GCANNNNNTGC	1 cut(s)	2028
BstC8I	GCNNGC	1 cut(s)	2024
BstDEI	CTNAG	9 cut(s)	33, 339, 801, 1059, 1067, 1368, 1518, 1656, 2219
BstDSI	CCRYGG	1 cut(s)	1272
BstF5I	GGATG	8 cut(s)	550, 728, 1048, 1318, 1351, 1579, 2045, 2254
BstHHI	GCGC	2 cut(s)	894, 915
BstKTI	GATC	7 cut(s)	306, 559, 644, 692, 1384, 1423, 1968
BstMAI	GTCTC	5 cut(s)	1074, 1381, 1548, 1990, 2414
BstMBI	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
BstMWI	GCNNNNNNNGC	7 cut(s)	285, 1055, 2028, 2180, 2294, 2303, 2312
BstNI	CCWGG	4 cut(s)	639, 771, 1137, 2324
BstNSI	RCATGY	2 cut(s)	577, 1234
BstSCI	CCNGG	5 cut(s)	586, 637, 769, 1135, 2322
BstSLI	GKGCMC	2 cut(s)	728, 2033
BstV1I	GCAGC	8 cut(s)	241, 568, 594, 1896, 1966, 1969, 2192, 2316
BstV2I	GAAGAC	2 cut(s)	22, 1138
BstX2I	RGATCY	2 cut(s)	641, 689
BstXI	CCANNNNNNTGG	1 cut(s)	909
BstYI	RGATCY	2 cut(s)	641, 689
Bsu15I	ATCGAT	1 cut(s)	302
BsuI	GTATCC	1 cut(s)	399
BsuRI	GGCC	6 cut(s)	279, 344, 1703, 1980, 2174, 2306
BsuTUI	ATCGAT	1 cut(s)	302
BtgI	CCRYGG	1 cut(s)	1272
BtgZI	GCGATG	3 cut(s)	303, 876, 2238
BtsCI	GGATG	8 cut(s)	550, 728, 1048, 1318, 1351, 1579, 2045, 2254
BtsI	GCAGTG	3 cut(s)	880, 887, 1605
BtsIMutI	CAGTG	7 cut(s)	153, 880, 887, 1041, 1575, 1605, 2307
BveI	ACCTGC	2 cut(s)	259, 1028
Cac8I	GCNNGC	1 cut(s)	2024
CaiI	CAGNNNCTG	2 cut(s)	2274, 2329
CciI	TCATGA	3 cut(s)	463, 751, 1417
CfoI	GCGC	2 cut(s)	894, 915
Cfr10I	RCCGGY	1 cut(s)	2236
Cfr13I	GGNCC	3 cut(s)	1450, 2173, 2405
ClaI	ATCGAT	1 cut(s)	302
CspAI	ACCGGT	1 cut(s)	2236
CspCI	CAANNNNNGTGG	2 cut(s)	1733, 1768
DdeI	CTNAG	9 cut(s)	33, 339, 801, 1059, 1067, 1368, 1518, 1656, 2219
DpnI	GATC	7 cut(s)	305, 558, 643, 691, 1383, 1422, 1967
DpnII	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
DraI	TTTAAA	1 cut(s)	685
DraIII	CACNNNGTG	1 cut(s)	2028
DrdI	GACNNNNNNGTC	2 cut(s)	24, 1540
DseDI	GACNNNNNNGTC	2 cut(s)	24, 1540
EaeI	YGGCCR	2 cut(s)	277, 2304
Eam1104I	CTCTTC	3 cut(s)	204, 542, 651
EarI	CTCTTC	3 cut(s)	204, 542, 651
Eco130I	CCWWGG	1 cut(s)	383
Eco32I	GATATC	1 cut(s)	907
Eco47I	GGWCC	2 cut(s)	1450, 2405
Eco57I	CTGAAG	6 cut(s)	228, 317, 494, 938, 1377, 1916
Eco88I	CYCGRG	1 cut(s)	792
EcoRI	GAATTC	1 cut(s)	1814
EcoRII	CCWGG	4 cut(s)	637, 769, 1135, 2322
EcoRV	GATATC	1 cut(s)	907
EcoT14I	CCWWGG	1 cut(s)	383
EcoT22I	ATGCAT	1 cut(s)	2254
ErhI	CCWWGG	1 cut(s)	383
FaqI	GGGAC	1 cut(s)	2067
FauI	CCCGC	1 cut(s)	1711
FbaI	TGATCA	1 cut(s)	1420
FblI	GTMKAC	1 cut(s)	1523
Fnu4HI	GCNGC	8 cut(s)	255, 582, 608, 1885, 1955, 1958, 2206, 2330
FokI	GGATG	8 cut(s)	537, 715, 1055, 1325, 1358, 1586, 2032, 2261
Fsp4HI	GCNGC	8 cut(s)	255, 582, 608, 1885, 1955, 1958, 2206, 2330
FspBI	CTAG	5 cut(s)	504, 542, 2289, 2333, 2411
FspI	TGCGCA	1 cut(s)	893
GlaI	GCGC	2 cut(s)	893, 914
GluI	GCNGC	8 cut(s)	255, 582, 608, 1885, 1955, 1958, 2206, 2330
GsuI	CTGGAG	2 cut(s)	885, 2296
HaeIII	GGCC	6 cut(s)	279, 344, 1703, 1980, 2174, 2306
HapII	CCGG	5 cut(s)	122, 587, 1207, 1431, 2237
HhaI	GCGC	2 cut(s)	894, 915
Hin6I	GCGC	2 cut(s)	892, 913
HinP1I	GCGC	2 cut(s)	892, 913
HincII	GTYRAC	2 cut(s)	160, 268
HindII	GTYRAC	2 cut(s)	160, 268
HindIII	AAGCTT	1 cut(s)	1112
HpaI	GTTAAC	2 cut(s)	160, 268
HpaII	CCGG	5 cut(s)	122, 587, 1207, 1431, 2237
HphI	GGTGA	7 cut(s)	368, 698, 890, 1304, 2224, 2240, 2251
Hpy166II	GTNNAC	8 cut(s)	160, 268, 726, 887, 1179, 1524, 1583, 2408
Hpy8I	GTNNAC	8 cut(s)	160, 268, 726, 887, 1179, 1524, 1583, 2408
HpyCH4III	ACNGT	9 cut(s)	157, 765, 851, 1570, 1795, 1823, 2169, 2311, 2405
HpyCH4IV	ACGT	2 cut(s)	546, 1396
HpyCH4V	TGCA	9 cut(s)	584, 726, 873, 1401, 1598, 1744, 1946, 2128, 2252
HpyF10VI	GCNNNNNNNGC	7 cut(s)	285, 1055, 2028, 2180, 2294, 2303, 2312
HpyF3I	CTNAG	9 cut(s)	33, 339, 801, 1059, 1067, 1368, 1518, 1656, 2219
HpySE526I	ACGT	2 cut(s)	546, 1396
HspAI	GCGC	2 cut(s)	892, 913
KpnI	GGTACC	1 cut(s)	530
Ksp22I	TGATCA	1 cut(s)	1420
KspAI	GTTAAC	2 cut(s)	160, 268
Kzo9I	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
LmnI	GCTCC	4 cut(s)	904, 1433, 1651, 2326
Lsp1109I	GCAGC	8 cut(s)	241, 568, 594, 1896, 1966, 1969, 2192, 2316
LweI	GCATC	9 cut(s)	79, 638, 1336, 1564, 1692, 1731, 1815, 2239, 2261
MaeI	CTAG	5 cut(s)	504, 542, 2289, 2333, 2411
MaeII	ACGT	2 cut(s)	546, 1396
MaeIII	GTNAC	3 cut(s)	151, 742, 1564
MalI	GATC	7 cut(s)	305, 558, 643, 691, 1383, 1422, 1967
MboI	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
MfeI	CAATTG	1 cut(s)	2259
MflI	RGATCY	2 cut(s)	641, 689
MhlI	GDGCHC	2 cut(s)	728, 2033
MlsI	TGGCCA	2 cut(s)	279, 2306
MluCI	AATT	8 cut(s)	281, 359, 415, 1478, 1705, 1814, 1939, 2259
MluNI	TGGCCA	2 cut(s)	279, 2306
MlyI	GAGTC	4 cut(s)	1076, 1253, 1311, 1523
MmeI	TCCRAC	3 cut(s)	132, 472, 756
Mox20I	TGGCCA	2 cut(s)	279, 2306
Mph1103I	ATGCAT	1 cut(s)	2254
MroXI	GAANNNNTTC	2 cut(s)	1839, 1929
MscI	TGGCCA	2 cut(s)	279, 2306
Msp20I	TGGCCA	2 cut(s)	279, 2306
MspA1I	CMGCKG	1 cut(s)	607
MspCI	CTTAAG	2 cut(s)	1142, 1961
MspI	CCGG	5 cut(s)	122, 587, 1207, 1431, 2237
MspR9I	CCNGG	5 cut(s)	588, 639, 771, 1137, 2324
MunI	CAATTG	1 cut(s)	2259
Mva1269I	GAATGC	1 cut(s)	409
MvaI	CCWGG	4 cut(s)	639, 771, 1137, 2324
MwoI	GCNNNNNNNGC	7 cut(s)	285, 1055, 2028, 2180, 2294, 2303, 2312
NciI	CCSGG	1 cut(s)	588
NdeII	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
NlaIV	GGNNCC	7 cut(s)	106, 144, 528, 592, 900, 1435, 1653
NmeAIII	GCCGAG	1 cut(s)	2371
NmuCI	GTSAC	2 cut(s)	151, 742
NsbI	TGCGCA	1 cut(s)	893
NsiI	ATGCAT	1 cut(s)	2254
NspI	RCATGY	2 cut(s)	577, 1234
PagI	TCATGA	3 cut(s)	463, 751, 1417
PciI	ACATGT	1 cut(s)	573
PctI	GAATGC	1 cut(s)	409
PdmI	GAANNNNTTC	2 cut(s)	1839, 1929
PfeI	GAWTC	9 cut(s)	232, 397, 1063, 1556, 1622, 1835, 2065, 2215, 2396
PflMI	CCANNNNNTGG	1 cut(s)	1850
PinAI	ACCGGT	1 cut(s)	2236
PkrI	GCNGC	8 cut(s)	256, 583, 609, 1886, 1956, 1959, 2207, 2331
PleI	GAGTC	4 cut(s)	1076, 1253, 1311, 1523
PpsI	GAGTC	4 cut(s)	1076, 1253, 1311, 1523
PscI	ACATGT	1 cut(s)	573
PshBI	ATTAAT	1 cut(s)	831
Psp1406I	AACGTT	1 cut(s)	1396
Psp6I	CCWGG	4 cut(s)	637, 769, 1135, 2322
PspGI	CCWGG	4 cut(s)	637, 769, 1135, 2322
PspN4I	GGNNCC	7 cut(s)	106, 144, 528, 592, 900, 1435, 1653
PspPI	GGNCC	3 cut(s)	1450, 2173, 2405
PstNI	CAGNNNCTG	2 cut(s)	2274, 2329
PsuI	RGATCY	2 cut(s)	641, 689
PvuII	CAGCTG	1 cut(s)	607
SatI	GCNGC	8 cut(s)	255, 582, 608, 1885, 1955, 1958, 2206, 2330
Sau3AI	GATC	7 cut(s)	303, 556, 641, 689, 1381, 1420, 1965
Sau96I	GGNCC	3 cut(s)	1450, 2173, 2405
ScaI	AGTACT	1 cut(s)	2091
SchI	GAGTC	4 cut(s)	1076, 1253, 1311, 1523
ScrFI	CCNGG	5 cut(s)	588, 639, 771, 1137, 2324
SduI	GDGCHC	2 cut(s)	728, 2033
SfaNI	GCATC	9 cut(s)	79, 638, 1336, 1564, 1692, 1731, 1815, 2239, 2261
SinI	GGWCC	2 cut(s)	1450, 2405
SmlI	CTYRAG	5 cut(s)	452, 611, 1142, 1253, 1961
SmoI	CTYRAG	5 cut(s)	452, 611, 1142, 1253, 1961
Sse9I	AATT	8 cut(s)	281, 359, 415, 1478, 1705, 1814, 1939, 2259
SsiI	CCGC	3 cut(s)	1448, 1718, 2246
SspI	AATATT	2 cut(s)	179, 499
SspMI	CTAG	5 cut(s)	504, 542, 2289, 2333, 2411
StyD4I	CCNGG	5 cut(s)	586, 637, 769, 1135, 2322
StyI	CCWWGG	1 cut(s)	383
TaaI	ACNGT	9 cut(s)	157, 765, 851, 1570, 1795, 1823, 2169, 2311, 2405
TaiI	ACGT	2 cut(s)	549, 1399
TaqI	TCGA	5 cut(s)	171, 302, 424, 1532, 1709
TasI	AATT	8 cut(s)	281, 359, 415, 1478, 1705, 1814, 1939, 2259
TatI	WGTACW	1 cut(s)	2089
TfiI	GAWTC	9 cut(s)	232, 397, 1063, 1556, 1622, 1835, 2065, 2215, 2396
TscAI	CASTG	7 cut(s)	160, 880, 894, 1041, 1575, 1605, 2314
TseFI	GTSAC	2 cut(s)	151, 742
TseI	GCWGC	8 cut(s)	254, 581, 607, 1884, 1954, 1957, 2205, 2329
Tsp45I	GTSAC	2 cut(s)	151, 742
TspGWI	ACGGA	5 cut(s)	85, 858, 1289, 1703, 2027
TspRI	CASTG	7 cut(s)	160, 880, 894, 1041, 1575, 1605, 2314
Van91I	CCANNNNNTGG	1 cut(s)	1850
Vha464I	CTTAAG	2 cut(s)	1142, 1961
VneI	GTGCAC	1 cut(s)	724
VpaK11BI	GGWCC	2 cut(s)	1450, 2405
VspI	ATTAAT	1 cut(s)	831
XapI	RAATTY	3 cut(s)	1478, 1814, 1939
XceI	RCATGY	2 cut(s)	577, 1234
XmiI	GTMKAC	1 cut(s)	1523
XmnI	GAANNNNTTC	2 cut(s)	1839, 1929
XspI	CTAG	5 cut(s)	504, 542, 2289, 2333, 2411
ZrmI	AGTACT	1 cut(s)	2091
Zsp2I	ATGCAT	1 cut(s)	2254

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.