Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0009254

Physical Location & Seq

Genomic Coordinates Forward (+)

25289397 .. 25296738

Gene Structure 7342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG234500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGGCAGAGGCAGAGGAGGTTCACGCACTCAGAGACGCCACTTCCGTCAGAACAGAGAGAACGTCTGGAAGCGCACCAAGCCCGATCCTTCCTCTGAAAACAATGGCGCCGCCGCCGACCCTTCTTGGCAGCCCTTTGCTACCCAAAGCCCGTCATTCGAGGAGTACTATAAGGAGCAAGGGATAGTTGCCCCAGAAGAGTGGGATTTGTTCATGGAAGTTCTTCGAAAGCCGTTGCCGGCTGCTTTTAGGATCAATTCAAGTAGCCAGTTTTGTTCCGATATCCGAACCCAGTTGGAGAGTGATTTTATGATATCTCTTCAAGCTGAGGTGAGTGAAGGGGGTGAACTGCAGCCTATCCGGCCATTGCCTTGGTACCCTGAGAATCTTGCTTGGCAGTCAAATTTTTCTCGCATGCAGCTGAGGAAGAACCAAACTCTTGAGAGGTTTCATGAGTTTTTAAAGCTTGAAAATGAGATTGGAAACATCACAAGACAGGAGGCTGTCAGCATGGTGCCTCCTCTATTCCTTGATGTACGTCCAGAACATTTTATACTTGATATGTGTGCTGCACCAGGTTCAAAAACATTCCAATTGCTTGAGATTATACACAGATCAACAAAACCAGGACTCCTACCTGATGGATTGGTTGTAGCAAATGATCTTGACGTTCAAAGGTGCAATCTACTCATCCATCAAACAAAAAGAATGTGCACAGCTAACTTGATAGTCACAAACCATGAAGCTCAGCACTTCCCTGGCTGCCGTTCACACAAAAAAAGCTCTAGTGCTTCTGAAATAGGAACTGAATTGGAGCCACCCATCAGTCAACTTCTCTTTGATCGTGTTTTATGTGATGTCCCTTGTAGTGGCGATGGTACCCTCCGCAAGGCTCCTGATATCTGGAGGAAATGGCATGCAGGTAATGGCAATGGGCTGCATGCTCTACAAGTTCAGATAGCGATGCGAGGTTTGTCTTTGCTGAAAGTTGGTGGAAGAATGGTTTATTCTACCTGCTCTATGAATCCAGTTGAGAATGAGGCTGTGATTTCTGAGATTTTGAGGAAATGTGGTGGATCTGTTGAGCTTATTGACGTCTCTAGTGAGCTTCCTCAACTGGTCAGGCGGCCAGGTCTTAAAAAATGGAAGGTACGTGACAAGGGTCGATGGTTAGTTTCCTCTAAAGATGTTGGCAAATATCGCACAAATGCCATTGTCCCTGGCATGTTTCCATCTGGAAGAAGCCTTGCAGTATCAATGGACCACAGTGGTAGTATGGATCAGGAAGAAAAGCAGGAGAATGGTGAATATGGAAATGTTGAAGATGCATTGCAGTCAAGTGAGGATGCTGTAATTTCAGCCAGTGAAGTAGACGAGGAAGTTTCTTTGTTTCCACTAGAACGTTGCATGAGGATAGTCCCACACGATCAAAATGGGGGAGCTTTCTTCATTGCAGTCCTCCACAAGCATTCCCCTTTGCCAGCCAATCAGGGGACAGCTTGTCCTGAAGGTGACTTGTGCTCTACAACTGACGAGCTGATTGTAAAATTACCAACTCAGGATACTGAAGATACAAATGGGTTGCTAGCTAATTTGGCAGATGATACAGATGAGACAACTACAGAAGCTGCCTCAGAGGCAGAGTTAATTGAGGGTGAACAAGAGGGGGCTACTTTGGAACCTGATACTACCATAACGAGTGAAGAAAATGTATCAGAGGCAAACCAAGTACCTGGTGATGTGGAAGCTGATCCAAAGAAAACTCGAGGAAAAAGAAAGCAGCTTCAGGGCAAGTGGAAAGGAGTTGACCCTGTTGTATTCTTTAAAGATGAAGCTGTCATCAATGCCATAAAGACATTTTATGGAATTGATGAATCATTTCCATTCAACGGCCACCTTGTCACAAGAAATAGTGATACCAATCATGTGAAGAGAATTTACTACGTCTCAAAGCCAGTCAAAGATGTTCTTGAACTGAATTTCTCTGTTGGAGAGCAACTTAAGATAACATCCATTGGGCTTAAGATGTTTGAACGACAAACTGCAAGTGTTGCAAGTTGTGCACCTTGTGCATTCCGGATATCATCTGAAGGATTGCCACTTATTCTTCCGTACATCACCAAACAGATTCTATATGCAACCCCAGTGGACTTCAAGCATATTCTTCAATATAAAAGTATCAAGTTTTCTGATTTTGTTGATGCTGAGTTCGGTGTGAAGGCATCAGGTTTAATGCTAGGGTGCTGTGTGTTAGTTTTGAGCAAAGGTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltr_RsmB-F

Protein Analysis

757

Amino Acids

84.21

Weight (kDa)

5.56

Isoelectric Point (pI)

44.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltr_RsmB-F	PF01189	176 - 364	3.6e-34	16S rRNA methyltransferase RsmB/F
Pre-PUA_NSUN2	PF25376	604 - 707	2.6e-34	NSUN2 Pre-PUA domain
PUA_NSUN2	PF25378	709 - 756	4.1e-12	NSUN2 PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22400 AT4G40000 AT4G40000
fragaria_vesca	FvH4_1g20370 FvH4_1g20370 FvH4_1g20370
malus_domestica	MD01G1034300.v1.1 MD15G1321600.v1.1
prunus_persica	Prupe.6G191700_v2.0.a1 Prupe.6G191700_v2.0.a1
pyrus_communis	pycom01g06140 pycom15g28220
rosa_chinensis	RchiOBHm_Chr2g0112501
rosa_laevigata	RLG00000017986
rosa_multiflora	Rmu_sc0001499.1_g000061
rosa_roxburghii	Rroxscaffold_2G00131370
rosa_rugosa	Rorug02G0181300
rosa_samantha	Rh2AG234500 Rh2BG248100 Rh2CG239100 Rh2DG242100
rosa_wichuraiana	Rw2G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1098
Acc36I	ACCTGC	2 cut(s)	911, 1022
Acc65I	GGTACC	2 cut(s)	375, 878
AccB1I	GGYRCC	4 cut(s)	107, 375, 514, 878
AccI	GTMKAC	1 cut(s)	1371
AccIII	TCCGGA	1 cut(s)	2076
AciI	CCGC	4 cut(s)	111, 114, 886, 1126
AclI	AACGTT	1 cut(s)	1402
AclWI	GGATC	5 cut(s)	80, 260, 1084, 1287, 1745
AcoI	YGGCCR	3 cut(s)	362, 1127, 1891
AcsI	RAATTY	3 cut(s)	403, 1935, 1978
AcuI	CTGAAG	4 cut(s)	1527, 1587, 1769, 2109
AcyI	GRCGYC	3 cut(s)	37, 108, 1095
AdeI	CACNNNGTG	1 cut(s)	2069
AfaI	GTAC	8 cut(s)	167, 377, 537, 880, 1152, 1731, 2114, 2269
AfiI	CCNNNNNNNGG	4 cut(s)	869, 889, 1491, 1889
AflII	CTTAAG	2 cut(s)	2000, 2021
AhdI	GACNNNNNGTC	1 cut(s)	1500
AjnI	CCWGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
AjuI	GAANNNNNNNTTGG	2 cut(s)	462, 494
Alw21I	GWGCWC	3 cut(s)	716, 1523, 2065
Alw26I	GTCTC	4 cut(s)	28, 1102, 1607, 1951
Alw44I	GTGCAC	2 cut(s)	712, 2061
AlwI	GGATC	5 cut(s)	80, 260, 1084, 1287, 1745
AlwNI	CAGNNNCTG	1 cut(s)	1628
Ama87I	CYCGRG	1 cut(s)	1764
Aor13HI	TCCGGA	1 cut(s)	2076
AoxI	GGCC	3 cut(s)	362, 1127, 1891
ApaLI	GTGCAC	2 cut(s)	712, 2061
ApeKI	GCWGC	9 cut(s)	130, 242, 352, 418, 569, 762, 937, 1628, 1780
ApoI	RAATTY	3 cut(s)	403, 1935, 1978
Asp700I	GAANNNNTTC	2 cut(s)	222, 1878
Asp718I	GGTACC	2 cut(s)	375, 878
AspLEI	GCGC	2 cut(s)	75, 110
AspS9I	GGNCC	1 cut(s)	1261
AsuHPI	GGTGA	7 cut(s)	343, 356, 1316, 1523, 1667, 1748, 2110
AsuII	TTCGAA	1 cut(s)	226
AsuNHI	GCTAGC	1 cut(s)	1585
AvaI	CYCGRG	1 cut(s)	1764
AvaII	GGWCC	1 cut(s)	1261
BaeGI	GKGCMC	2 cut(s)	716, 2065
BanI	GGYRCC	4 cut(s)	107, 375, 514, 878
BarI	GAAGNNNNNNTAC	2 cut(s)	1922, 1954
Bbv12I	GWGCWC	3 cut(s)	716, 1523, 2065
BbvCI	CCTCAGC	2 cut(s)	327, 422
BbvI	GCAGC	9 cut(s)	142, 229, 364, 430, 556, 749, 924, 1615, 1792
BccI	CCATC	6 cut(s)	635, 702, 830, 869, 1161, 1240
BceAI	ACGGC	3 cut(s)	217, 750, 1906
BciT130I	CCWGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
BciVI	GTATCC	1 cut(s)	1555
BcoDI	GTCTC	4 cut(s)	28, 1102, 1607, 1951
BfaI	CTAG	5 cut(s)	786, 1101, 1397, 1586, 2237
BfmI	CTRYAG	2 cut(s)	350, 1620
BfoI	RGCGCY	1 cut(s)	111
BfrI	CTTAAG	2 cut(s)	2000, 2021
BfuAI	ACCTGC	2 cut(s)	911, 1022
BfuI	GTATCC	1 cut(s)	1555
BglI	GCCNNNNNGGC	2 cut(s)	361, 1637
BlpI	GCTNAGC	1 cut(s)	747
BmcAI	AGTACT	1 cut(s)	167
Bme1390I	CCNGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
Bme18I	GGWCC	1 cut(s)	1261
BmeRI	GACNNNNNGTC	1 cut(s)	1500
BmeT110I	CYCGRG	1 cut(s)	1764
BmgT120I	GGNCC	1 cut(s)	1261
BmiI	GGNNCC	7 cut(s)	109, 377, 516, 816, 880, 894, 1680
BmrFI	CCNGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
BmrI	ACTGGG	2 cut(s)	286, 2138
BmsI	GCATC	5 cut(s)	954, 1315, 1336, 2191, 2231
BmtI	GCTAGC	1 cut(s)	1589
BmuI	ACTGGG	2 cut(s)	286, 2138
BoxI	GACNNNNGTC	1 cut(s)	1161
BpmI	CTGGAG	1 cut(s)	925
Bpu10I	CCTNAGC	2 cut(s)	327, 422
Bpu1102I	GCTNAGC	1 cut(s)	747
Bpu14I	TTCGAA	1 cut(s)	226
BpuEI	CTTGAG	2 cut(s)	461, 620
BsaAI	YACGTR	1 cut(s)	1154
BsaBI	GATNNNNATC	1 cut(s)	1920
BsaHI	GRCGYC	3 cut(s)	37, 108, 1095
BsaJI	CCNNGG	3 cut(s)	371, 757, 1219
BsaWI	WCCGGW	1 cut(s)	2076
BsaXI	ACNNNNNCTCC	2 cut(s)	615, 645
Bsc4I	CCNNNNNNNGG	4 cut(s)	869, 889, 1491, 1889
Bse118I	RCCGGY	1 cut(s)	238
Bse1I	ACTGG	7 cut(s)	268, 292, 1028, 1122, 1362, 1955, 2144
Bse3DI	GCAATG	4 cut(s)	365, 937, 1328, 1449
Bse8I	GATNNNNATC	1 cut(s)	1920
BseAI	TCCGGA	1 cut(s)	2076
BseBI	CCWGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
BseDI	CCNNGG	3 cut(s)	371, 757, 1219
BseGI	GGATG	3 cut(s)	690, 1351, 2009
BseJI	GATNNNNATC	1 cut(s)	1920
BseLI	CCNNNNNNNGG	4 cut(s)	869, 889, 1491, 1889
BseMI	GCAATG	4 cut(s)	365, 937, 1328, 1449
BseMII	CTCAG	9 cut(s)	44, 318, 372, 413, 761, 1044, 1571, 1647, 2196
BseNI	ACTGG	7 cut(s)	268, 292, 1028, 1122, 1362, 1955, 2144
BseRI	GAGGAG	3 cut(s)	30, 176, 510
BseSI	GKGCMC	2 cut(s)	716, 2065
BseXI	GCAGC	9 cut(s)	142, 229, 364, 430, 556, 749, 924, 1615, 1792
BsgI	GTGCAG	1 cut(s)	555
BshFI	GGCC	3 cut(s)	364, 1129, 1893
BshNI	GGYRCC	4 cut(s)	107, 375, 514, 878
BsiHKAI	GWGCWC	3 cut(s)	716, 1523, 2065
BsiHKCI	CYCGRG	1 cut(s)	1764
BsiSI	CCGG	3 cut(s)	239, 361, 2077
BslFI	GGGAC	4 cut(s)	845, 1202, 1403, 1507
BslI	CCNNNNNNNGG	4 cut(s)	869, 889, 1491, 1889
BsmAI	GTCTC	4 cut(s)	28, 1102, 1607, 1951
BsmBI	CGTCTC	3 cut(s)	28, 1102, 1951
BsmFI	GGGAC	4 cut(s)	845, 1202, 1403, 1507
BsmI	GAATGC	2 cut(s)	1468, 2072
BsnI	GGCC	3 cut(s)	364, 1129, 1893
BsoBI	CYCGRG	1 cut(s)	1764
Bsp119I	TTCGAA	1 cut(s)	226
Bsp1286I	GDGCHC	3 cut(s)	716, 1523, 2065
Bsp13I	TCCGGA	1 cut(s)	2076
Bsp143I	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
Bsp1720I	GCTNAGC	1 cut(s)	747
BspACI	CCGC	4 cut(s)	111, 114, 886, 1126
BspANI	GGCC	3 cut(s)	364, 1129, 1893
BspCNI	CTCAG	9 cut(s)	43, 319, 373, 414, 760, 1045, 1570, 1646, 2197
BspEI	TCCGGA	1 cut(s)	2076
BspHI	TCATGA	1 cut(s)	451
BspLI	GGNNCC	7 cut(s)	109, 377, 516, 816, 880, 894, 1680
BspMAI	CTGCAG	1 cut(s)	354
BspMI	ACCTGC	2 cut(s)	911, 1022
BspOI	GCTAGC	1 cut(s)	1589
BspPI	GGATC	5 cut(s)	80, 260, 1084, 1287, 1745
BspT104I	TTCGAA	1 cut(s)	226
BspT107I	GGYRCC	4 cut(s)	107, 375, 514, 878
BspTI	CTTAAG	2 cut(s)	2000, 2021
BsrDI	GCAATG	4 cut(s)	365, 937, 1328, 1449
BsrFI	RCCGGY	1 cut(s)	238
BsrI	ACTGG	7 cut(s)	268, 292, 1028, 1122, 1362, 1955, 2144
BssAI	RCCGGY	1 cut(s)	238
BssECI	CCNNGG	3 cut(s)	371, 757, 1219
BssMI	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
BssNI	GRCGYC	3 cut(s)	37, 108, 1095
BssT1I	CCWWGG	1 cut(s)	371
Bst2UI	CCWGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
Bst4CI	ACNGT	1 cut(s)	1268
Bst6I	CTCTTC	3 cut(s)	192, 324, 1925
BstACI	GRCGYC	3 cut(s)	37, 108, 1095
BstAFI	CTTAAG	2 cut(s)	2000, 2021
BstAPI	GCANNNNNTGC	3 cut(s)	2051, 2060, 2069
BstBAI	YACGTR	1 cut(s)	1154
BstBI	TTCGAA	1 cut(s)	226
BstC8I	GCNNGC	6 cut(s)	240, 416, 918, 942, 1482, 1587
BstDEI	CTNAG	9 cut(s)	30, 327, 381, 422, 747, 1053, 1557, 1633, 2205
BstENI	CCTNNNNNAGG	1 cut(s)	887
BstF5I	GGATG	3 cut(s)	690, 1351, 2009
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	2 cut(s)	75, 110
BstKTI	GATC	9 cut(s)	88, 255, 617, 664, 844, 1079, 1282, 1429, 1753
BstMAI	GTCTC	4 cut(s)	28, 1102, 1607, 1951
BstMBI	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
BstMWI	GCNNNNNNNGC	8 cut(s)	79, 361, 1200, 1595, 1637, 2051, 2060, 2069
BstNI	CCWGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
BstNSI	RCATGY	4 cut(s)	418, 920, 944, 1228
BstPAI	GACNNNNGTC	1 cut(s)	1161
BstSCI	CCNGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
BstSFI	CTRYAG	2 cut(s)	350, 1620
BstSLI	GKGCMC	2 cut(s)	716, 2065
BstV1I	GCAGC	9 cut(s)	142, 229, 364, 430, 556, 749, 924, 1615, 1792
BstX2I	RGATCY	1 cut(s)	1076
BstXI	CCANNNNNNTGG	3 cut(s)	201, 372, 1733
BstYI	RGATCY	1 cut(s)	1076
BsuI	GTATCC	1 cut(s)	1555
BsuRI	GGCC	3 cut(s)	364, 1129, 1893
BtgZI	GCGATG	2 cut(s)	888, 977
BtsCI	GGATG	3 cut(s)	690, 1351, 2009
BtsIMutI	CAGTG	3 cut(s)	1273, 1369, 2151
BveI	ACCTGC	2 cut(s)	911, 1022
Cac8I	GCNNGC	6 cut(s)	240, 416, 918, 942, 1482, 1587
CaiI	CAGNNNCTG	1 cut(s)	1628
CciI	TCATGA	1 cut(s)	451
CfoI	GCGC	2 cut(s)	75, 110
Cfr10I	RCCGGY	1 cut(s)	238
Cfr13I	GGNCC	1 cut(s)	1261
CseI	GACGC	1 cut(s)	45
CsiI	ACCWGGT	2 cut(s)	574, 1732
Csp6I	GTAC	8 cut(s)	166, 376, 536, 879, 1151, 1730, 2113, 2268
CspCI	CAANNNNNGTGG	2 cut(s)	2127, 2162
CviQI	GTAC	8 cut(s)	166, 376, 536, 879, 1151, 1730, 2113, 2268
DdeI	CTNAG	9 cut(s)	30, 327, 381, 422, 747, 1053, 1557, 1633, 2205
DinI	GGCGCC	1 cut(s)	109
DpnI	GATC	9 cut(s)	87, 254, 616, 663, 843, 1078, 1281, 1428, 1752
DpnII	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
DraI	TTTAAA	2 cut(s)	462, 1825
DraIII	CACNNNGTG	1 cut(s)	2069
DriI	GACNNNNNGTC	1 cut(s)	1500
EaeI	YGGCCR	3 cut(s)	362, 1127, 1891
Eam1104I	CTCTTC	3 cut(s)	192, 324, 1925
Eam1105I	GACNNNNNGTC	1 cut(s)	1500
EarI	CTCTTC	3 cut(s)	192, 324, 1925
Eco130I	CCWWGG	1 cut(s)	371
Eco32I	GATATC	4 cut(s)	283, 315, 901, 2082
Eco47I	GGWCC	1 cut(s)	1261
Eco57I	CTGAAG	4 cut(s)	1527, 1587, 1769, 2109
Eco88I	CYCGRG	1 cut(s)	1764
EcoNI	CCTNNNNNAGG	1 cut(s)	887
EcoRII	CCWGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
EcoRV	GATATC	4 cut(s)	283, 315, 901, 2082
EcoT14I	CCWWGG	1 cut(s)	371
EcoT22I	ATGCAT	1 cut(s)	1330
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	1 cut(s)	371
Esp3I	CGTCTC	3 cut(s)	28, 1102, 1951
FalI	AAGNNNNNCTT	2 cut(s)	1953, 1985
FaqI	GGGAC	4 cut(s)	845, 1202, 1403, 1507
FblI	GTMKAC	1 cut(s)	1371
FokI	GGATG	3 cut(s)	677, 1358, 1996
FspBI	CTAG	5 cut(s)	786, 1101, 1397, 1586, 2237
GlaI	GCGC	2 cut(s)	74, 109
GsuI	CTGGAG	1 cut(s)	925
HaeII	RGCGCY	1 cut(s)	111
HaeIII	GGCC	3 cut(s)	364, 1129, 1893
HapII	CCGG	3 cut(s)	239, 361, 2077
HgaI	GACGC	1 cut(s)	45
HhaI	GCGC	2 cut(s)	75, 110
Hin1I	GRCGYC	3 cut(s)	37, 108, 1095
Hin6I	GCGC	2 cut(s)	73, 108
HinP1I	GCGC	2 cut(s)	73, 108
HincII	GTYRAC	2 cut(s)	830, 1807
HindII	GTYRAC	2 cut(s)	830, 1807
HindIII	AAGCTT	1 cut(s)	464
HinfI	GANTC	5 cut(s)	385, 630, 1024, 1874, 2128
HpaII	CCGG	3 cut(s)	239, 361, 2077
HphI	GGTGA	7 cut(s)	343, 356, 1316, 1523, 1667, 1748, 2110
HpyAV	CCTTC	7 cut(s)	99, 132, 332, 1141, 1502, 2084, 2212
HpyCH4III	ACNGT	1 cut(s)	1268
HpyCH4IV	ACGT	7 cut(s)	63, 538, 669, 1095, 1153, 1402, 1944
HpyF10VI	GCNNNNNNNGC	8 cut(s)	79, 361, 1200, 1595, 1637, 2051, 2060, 2069
HpyF3I	CTNAG	9 cut(s)	30, 327, 381, 422, 747, 1053, 1557, 1633, 2205
HpySE526I	ACGT	7 cut(s)	63, 538, 669, 1095, 1153, 1402, 1944
Hsp92I	GRCGYC	3 cut(s)	37, 108, 1095
HspAI	GCGC	2 cut(s)	73, 108
KasI	GGCGCC	1 cut(s)	107
Kpn2I	TCCGGA	1 cut(s)	2076
KpnI	GGTACC	2 cut(s)	379, 882
KroI	GCCGGC	1 cut(s)	238
KroNI	GCCGGC	1 cut(s)	240
Kzo9I	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
LmnI	GCTCC	4 cut(s)	175, 814, 898, 1439
Lsp1109I	GCAGC	9 cut(s)	142, 229, 364, 430, 556, 749, 924, 1615, 1792
LweI	GCATC	5 cut(s)	954, 1315, 1336, 2191, 2231
MabI	ACCWGGT	2 cut(s)	574, 1732
MaeI	CTAG	5 cut(s)	786, 1101, 1397, 1586, 2237
MaeII	ACGT	7 cut(s)	63, 538, 669, 1095, 1153, 1402, 1944
MaeIII	GTNAC	4 cut(s)	730, 1154, 1511, 1900
MalI	GATC	9 cut(s)	87, 254, 616, 663, 843, 1078, 1281, 1428, 1752
MboI	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
MfeI	CAATTG	1 cut(s)	593
MflI	RGATCY	1 cut(s)	1076
MhlI	GDGCHC	3 cut(s)	716, 1523, 2065
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	1 cut(s)	624
MmeI	TCCRAC	2 cut(s)	276, 1969
Mph1103I	ATGCAT	1 cut(s)	1330
MroI	TCCGGA	1 cut(s)	2076
MroNI	GCCGGC	1 cut(s)	238
MroXI	GAANNNNTTC	2 cut(s)	222, 1878
MseI	TTAA	7 cut(s)	461, 1137, 1646, 1824, 2001, 2022, 2231
MspA1I	CMGCKG	1 cut(s)	421
MspCI	CTTAAG	2 cut(s)	2000, 2021
MspI	CCGG	3 cut(s)	239, 361, 2077
MspR9I	CCNGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
MunI	CAATTG	1 cut(s)	593
Mva1269I	GAATGC	2 cut(s)	1468, 2072
MvaI	CCWGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
MwoI	GCNNNNNNNGC	8 cut(s)	79, 361, 1200, 1595, 1637, 2051, 2060, 2069
NaeI	GCCGGC	1 cut(s)	240
NarI	GGCGCC	1 cut(s)	108
NdeII	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
NgoMIV	GCCGGC	1 cut(s)	238
NheI	GCTAGC	1 cut(s)	1585
NlaIV	GGNNCC	7 cut(s)	109, 377, 516, 816, 880, 894, 1680
NmuCI	GTSAC	4 cut(s)	730, 1154, 1511, 1900
NsiI	ATGCAT	1 cut(s)	1330
NspI	RCATGY	4 cut(s)	418, 920, 944, 1228
NspV	TTCGAA	1 cut(s)	226
PaeI	GCATGC	3 cut(s)	418, 920, 944
PaeR7I	CTCGAG	1 cut(s)	1764
PagI	TCATGA	1 cut(s)	451
PcsI	WCGNNNNNNNCGW	1 cut(s)	43
PctI	GAATGC	2 cut(s)	1468, 2072
PdiI	GCCGGC	1 cut(s)	240
PdmI	GAANNNNTTC	2 cut(s)	222, 1878
PfeI	GAWTC	4 cut(s)	385, 1024, 1874, 2128
PleI	GAGTC	1 cut(s)	624
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	1 cut(s)	624
Ppu21I	YACGTR	1 cut(s)	1154
PshAI	GACNNNNGTC	1 cut(s)	1161
Psp1406I	AACGTT	1 cut(s)	1402
Psp6I	CCWGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
PspGI	CCWGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
PspN4I	GGNNCC	7 cut(s)	109, 377, 516, 816, 880, 894, 1680
PspPI	GGNCC	1 cut(s)	1261
PspXI	VCTCGAGB	1 cut(s)	1764
PsrI	GAACNNNNNNTAC	2 cut(s)	537, 569
PstI	CTGCAG	1 cut(s)	354
PstNI	CAGNNNCTG	1 cut(s)	1628
PsuI	RGATCY	1 cut(s)	1076
PvuII	CAGCTG	1 cut(s)	421
RsaI	GTAC	8 cut(s)	167, 377, 537, 880, 1152, 1731, 2114, 2269
RsaNI	GTAC	8 cut(s)	166, 376, 536, 879, 1151, 1730, 2113, 2268
SaqAI	TTAA	7 cut(s)	461, 1137, 1646, 1824, 2001, 2022, 2231
Sau3AI	GATC	9 cut(s)	85, 252, 614, 661, 841, 1076, 1279, 1426, 1750
Sau96I	GGNCC	1 cut(s)	1261
ScaI	AGTACT	1 cut(s)	167
SchI	GAGTC	1 cut(s)	624
ScrFI	CCNGG	6 cut(s)	576, 627, 759, 1131, 1221, 1734
SduI	GDGCHC	3 cut(s)	716, 1523, 2065
SexAI	ACCWGGT	2 cut(s)	574, 1732
SfaNI	GCATC	5 cut(s)	954, 1315, 1336, 2191, 2231
SfcI	CTRYAG	2 cut(s)	350, 1620
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	1 cut(s)	1764
SfuI	TTCGAA	1 cut(s)	226
SinI	GGWCC	1 cut(s)	1261
SlaI	CTCGAG	1 cut(s)	1764
SmlI	CTYRAG	5 cut(s)	440, 599, 1764, 2000, 2021
SmoI	CTYRAG	5 cut(s)	440, 599, 1764, 2000, 2021
SphI	GCATGC	3 cut(s)	418, 920, 944
SsiI	CCGC	4 cut(s)	111, 114, 886, 1126
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	5 cut(s)	786, 1101, 1397, 1586, 2237
StyD4I	CCNGG	6 cut(s)	574, 625, 757, 1129, 1219, 1732
StyI	CCWWGG	1 cut(s)	371
TaaI	ACNGT	1 cut(s)	1268
TaiI	ACGT	7 cut(s)	66, 541, 672, 1098, 1156, 1405, 1947
TaqI	TCGA	4 cut(s)	159, 226, 1165, 1765
TatI	WGTACW	1 cut(s)	165
TauI	GCSGC	3 cut(s)	113, 116, 1129
TfiI	GAWTC	4 cut(s)	385, 1024, 1874, 2128
Tru1I	TTAA	7 cut(s)	461, 1137, 1646, 1824, 2001, 2022, 2231
Tru9I	TTAA	7 cut(s)	461, 1137, 1646, 1824, 2001, 2022, 2231
TscAI	CASTG	3 cut(s)	1273, 1369, 2151
TseFI	GTSAC	4 cut(s)	730, 1154, 1511, 1900
TseI	GCWGC	9 cut(s)	130, 242, 352, 418, 569, 762, 937, 1628, 1780
Tsp45I	GTSAC	4 cut(s)	730, 1154, 1511, 1900
TspDTI	ATGAA	7 cut(s)	202, 440, 756, 1037, 1438, 1845, 1887
TspGWI	ACGGA	2 cut(s)	35, 2100
TspRI	CASTG	3 cut(s)	1273, 1369, 2151
Vha464I	CTTAAG	2 cut(s)	2000, 2021
VneI	GTGCAC	2 cut(s)	712, 2061
VpaK11BI	GGWCC	1 cut(s)	1261
XagI	CCTNNNNNAGG	1 cut(s)	887
XapI	RAATTY	3 cut(s)	403, 1935, 1978
XceI	RCATGY	4 cut(s)	418, 920, 944, 1228
XhoI	CTCGAG	1 cut(s)	1764
XmiI	GTMKAC	1 cut(s)	1371
XmnI	GAANNNNTTC	2 cut(s)	222, 1878
XspI	CTAG	5 cut(s)	786, 1101, 1397, 1586, 2237
ZraI	GACGTC	1 cut(s)	1096
ZrmI	AGTACT	1 cut(s)	167
Zsp2I	ATGCAT	1 cut(s)	1330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.