Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009254

Physical Location & Seq

Genomic Coordinates Reverse (-)

11707632 .. 11719713

Gene Structure 12082 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1034300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGACACACAAAATAGAAACCCTAGATCTAACAAAGAAACAAAGCAGCCCAAACCGAGACCCGGAAGCCCAAGAATCTGTTTGCAGTTCCCATAAAACTCACAGCGAGCGGAACAGCGAAACTCTTTTCGCTGAGACCAATCATCAAACAGTTAAGCTACGTACCCAAATGGGAGGCGGCGGCGGCAGAGGCAGAGGCGGTTCTCGCACTCAGCGACGCCACTTCCGTCAGAACAGAGAGAACGTTTGGAAGCGTCCCAAGTCTGATCCTTCCTCTGAAAACACCAATGAAAACAATGGCGATAATCGTGGTTGGGAACCCTTTGCCACTCAAAACCCTGCATTCGATGAATACTATAAGGAGCAAGGGATTGTGAGCCCAGAAGAGTGGGATGCATTCATTGAAGTTCTTCGAAAGCCGTTGCCTGCTGCTTTTAGAATCAATTCGAGTAGCCAATTTTGTTCGGATATCCGAAGGCAGTTGGAGGATGACTTTATGATCTCTCTGCAATCTGAGGTAAGTGAAGGGGGTGAACCACAGCCTATTAGGCCGTTGCCTTGGTACCCTGAGAATCTAGCTTGGCATTCAAATTTTTCTCGCATGCAGCTGAGGAAGAACCAAACACTTGAGAGGTTTCATGAGTTCTTAAAGCTTGAAAATGAGATTGGAAACATCACAAGACAGGAGGCTGTCAGCATGGTACCTCCACTATTCCTTGATGTACGTCCAGATCATTTTGTACTTGATATGTGTGCTGCACCAGGTTCAAAAACATTCCAATTGCTTGAGATTATACACCGATCAATGAAACCAGGATCCCTACCTGATGGATTGGTTGTAGCAAATGATCTTGATGTCCAAAGATGTAATCTACTCATCCATCAAACAAAACGAATGTGCACAGCCAACTTGATAGTCACAAATCATGAGGCTCAGCACTTTCCTGGCTGCCGGGCAAAGTCTACTGCTTCTGAAATAGGAGCTGAAATGGAGCCACCCATCAGCCAACTTGTGTTTGATCGTGTTTTATGTGATGTTCCTTGCAGTGGAGATGGTACCCTTCGCAAGGCTCCTGATATATGGAGGAAATGGCATGCTGGATTGGGCAATGGGGTTCATCCTCTACAAATTCAAATAGCTATGCGAGGTTTATCATTGCTTAAAGTTGGTGGAAGAATGGTGTACTCTACCTGCTCTATGAATCCAGTTGAGAATGAAGCCGTGGTTGCTGAGATTTTGCGGAGGGGTGGTGGGTCTGTTGAGCTTATTGATGTCTCTAGTGAGCTGCCTCAACTTGTTCGAAGGCCAGGTCTTAAAAAATGGAAGATACGTGACAAGGGTAGGTGGTTAGCTTCCCACAAAAATGTCAGCAAATATCGTAAAAGTGTCATTGTTCCCAGCATGTTTCCTTCTGGAAGAAGCTTTACAGAACAAATGGACGCTAATGGTAGTATGGAACTGGGAGAAAAGCATGAGAATGGTGGAAATGTTGAAGATGCATTGCAGTCAACTGATGATCCTGCAACTGTAGCCAATGAACAAGAGGAGGAAGTTTCTGATTTTCCACTAGAACGCTGCATGAGGATAGTCCCGCATGATCAAAACGGAGGAGCTTTCTTTATTGCTGTCTTCCACAAACATTCTGCTTTGCCAGCCACTCAGGGGAAACCTAGTAGTCCTAAAGGGGACTTGGACTCGAGAAATGATGAGCCGGAGGTGGAATTACAAAATCAGGGTAACCAAGATACAAATGGGATAGTGGTTAGTTTGGAAGATGGTACAGATGAGACTTTTTCAGAAGCAGCTTCAGAGGCAGAGTTAATAAAGGATGAACTAGATGGGGTGTCTTTGGAACTGGATCCTTCTGTCACACTTGAAGAAAATGTATCGGAGAAAGCCGAAGAGCCTGTTGATGAGGAAATTGATCCCAAAAAAGCCGGAGGAAAGAGAAAGAAGCAGTGTCAAGGCAAGTGGAGAGGAGTTGACCCTGTTGTTTTCTTTAAAGATGAAGCTACCATCAATAGCATAAAGACATTCTATGGCATTGATGAATCGTTTCCCTTCAATGACCACCTTGTTACAAGAAACACCGATGCTAATCATGTGAAGAGAATTTATTACATCTCAAAGTCGGTCAAAGACGTTCTTGAGCTGAATTTCTCCGTTGGACAGCAACTTAAGATAACATCCATTGGGCTTAAAATGTTTGAACGACAGACTGCAAGAGAAGGCAATTTGGCACCTTGTTTATTCCGGATATCATCAGAAGGGTTGCCACTTATTCTTCCATACATCACCAAACAGATTCTATGTGCATCCCTCGTGGACTTTAAGCATCTTCTCGAGTATAAATCTATCAAGTTTGCTGATTTTGTTGATTCTGAGTTTGGTCAGAAGGCATCAGGCATAATGTCAGGATGCTGTGTGATAGTTTTGAGTAAAGATGGCAAAGCCACGTCGGGTCCAATTGAAGTAGAGGAGTCAACAATAGCCATTGGATGCTGGAAAGGTAGGTCAAGTTTGTCTGTGATGGTCACTGCAATTGACTGCCAAGAACTGCAAGAAAGGCTTTTGGCACGCATGGAAACCGAGAAGGGACCTTTGGGGAAGAAAAACGAGGCTTCTAATGTCAACGGGTATGAGGTACAGGACACGAAGCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltr_RsmB-F

Protein Analysis

888

Amino Acids

99.18

Weight (kDa)

5.69

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltr_RsmB-F	PF01189	238 - 416	7.5e-34	16S rRNA methyltransferase RsmB/F
Pre-PUA_NSUN2	PF25376	663 - 766	8e-35	NSUN2 Pre-PUA domain
PUA_NSUN2	PF25378	768 - 858	2.9e-26	NSUN2 PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22400 AT4G40000 AT4G40000
fragaria_vesca	FvH4_1g20370 FvH4_1g20370 FvH4_1g20370
malus_domestica	MD01G1034300.v1.1 MD15G1321600.v1.1
prunus_persica	Prupe.6G191700_v2.0.a1 Prupe.6G191700_v2.0.a1
pyrus_communis	pycom01g06140 pycom15g28220
rosa_chinensis	RchiOBHm_Chr2g0112501
rosa_laevigata	RLG00000017986
rosa_multiflora	Rmu_sc0001499.1_g000061
rosa_roxburghii	Rroxscaffold_2G00131370
rosa_rugosa	Rorug02G0181300
rosa_samantha	Rh2AG234500 Rh2BG248100 Rh2CG239100 Rh2DG242100
rosa_wichuraiana	Rw2G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1199
Acc65I	GGTACC	3 cut(s)	561, 700, 1055
AccB1I	GGYRCC	4 cut(s)	561, 700, 1055, 2239
AccBSI	CCGCTC	1 cut(s)	109
AccI	GTMKAC	1 cut(s)	962
AccIII	TCCGGA	1 cut(s)	2253
AciI	CCGC	7 cut(s)	109, 177, 180, 183, 198, 1240, 1592
AclI	AACGTT	1 cut(s)	243
AclWI	GGATC	7 cut(s)	260, 810, 823, 1511, 1853, 1866, 1919
AcsI	RAATTY	4 cut(s)	589, 1128, 2112, 2155
AcuI	CTGAAG	1 cut(s)	1791
AcyI	GRCGYC	1 cut(s)	217
AfaI	GTAC	9 cut(s)	163, 563, 702, 723, 741, 1057, 1184, 1780, 2646
AfiI	CCNNNNNNNGG	4 cut(s)	61, 1046, 1066, 1662
AflII	CTTAAG	1 cut(s)	2177
AjiI	CACGTC	1 cut(s)	2457
AjnI	CCWGG	4 cut(s)	760, 811, 943, 1306
AjuI	GAANNNNNNNTTGG	4 cut(s)	648, 680, 2585, 2617
Alw21I	GWGCWC	1 cut(s)	902
Alw26I	GTCTC	4 cut(s)	51, 128, 1279, 1781
Alw44I	GTGCAC	1 cut(s)	898
AlwI	GGATC	7 cut(s)	260, 810, 823, 1511, 1853, 1866, 1919
Ama87I	CYCGRG	2 cut(s)	1696, 2342
Aor13HI	TCCGGA	1 cut(s)	2253
AoxI	GGCC	2 cut(s)	548, 1304
ApaLI	GTGCAC	1 cut(s)	898
ApeKI	GCWGC	8 cut(s)	45, 428, 604, 755, 948, 1285, 1575, 1802
ApoI	RAATTY	4 cut(s)	589, 1128, 2112, 2155
ArsI	GACNNNNNNTTYG	2 cut(s)	1294, 1326
Asp700I	GAANNNNTTC	3 cut(s)	408, 442, 2055
Asp718I	GGTACC	3 cut(s)	561, 700, 1055
AspS9I	GGNCC	2 cut(s)	2462, 2597
AsuC2I	CCSGG	2 cut(s)	62, 953
AsuHPI	GGTGA	2 cut(s)	542, 2287
AsuII	TTCGAA	2 cut(s)	412, 1300
AvaI	CYCGRG	2 cut(s)	1696, 2342
AvaII	GGWCC	2 cut(s)	2462, 2597
BaeGI	GKGCMC	1 cut(s)	902
BamHI	GGATCC	2 cut(s)	815, 1858
BanI	GGYRCC	4 cut(s)	561, 700, 1055, 2239
BanII	GRGCYC	1 cut(s)	380
BarI	GAAGNNNNNNTAC	2 cut(s)	1408, 1440
BauI	CACGAG	1 cut(s)	2321
BbsI	GAAGAC	1 cut(s)	1621
Bbv12I	GWGCWC	1 cut(s)	902
BbvCI	CCTCAGC	1 cut(s)	608
BbvI	GCAGC	8 cut(s)	57, 415, 616, 742, 935, 1272, 1562, 1814
BccI	CCATC	9 cut(s)	821, 888, 1007, 1046, 1769, 1832, 2024, 2438, 2524
BceAI	ACGGC	3 cut(s)	403, 535, 1205
BciT130I	CCWGG	4 cut(s)	762, 813, 945, 1308
BclI	TGATCA	1 cut(s)	1597
BcnI	CCSGG	2 cut(s)	62, 953
BcoDI	GTCTC	4 cut(s)	51, 128, 1279, 1781
BfaI	CTAG	6 cut(s)	23, 575, 1278, 1568, 1671, 1835
BfmI	CTRYAG	1 cut(s)	1527
BfrI	CTTAAG	1 cut(s)	2177
BfuAI	ACCTGC	1 cut(s)	1199
BglI	GCCNNNNNGGC	1 cut(s)	547
BglII	AGATCT	1 cut(s)	25
BlpI	GCTNAGC	1 cut(s)	933
Bme1390I	CCNGG	6 cut(s)	62, 762, 813, 945, 953, 1308
Bme18I	GGWCC	2 cut(s)	2462, 2597
BmeT110I	CYCGRG	2 cut(s)	1696, 2342
BmgBI	CACGTC	1 cut(s)	2457
BmgT120I	GGNCC	2 cut(s)	2462, 2597
BmrFI	CCNGG	6 cut(s)	62, 762, 813, 945, 953, 1308
BmrI	ACTGGG	1 cut(s)	1469
BmsI	GCATC	8 cut(s)	382, 1486, 2083, 2324, 2344, 2408, 2408, 2489
BmuI	ACTGGG	1 cut(s)	1469
BpiI	GAAGAC	1 cut(s)	1621
Bpu10I	CCTNAGC	1 cut(s)	608
Bpu1102I	GCTNAGC	1 cut(s)	933
Bpu14I	TTCGAA	2 cut(s)	412, 1300
BpuEI	CTTGAG	3 cut(s)	647, 806, 2168
BpuMI	CCSGG	2 cut(s)	62, 953
BsaAI	YACGTR	2 cut(s)	161, 1331
BsaBI	GATNNNNATC	1 cut(s)	2097
BsaHI	GRCGYC	1 cut(s)	217
BsaI	GGTCTC	2 cut(s)	51, 128
BsaJI	CCNNGG	2 cut(s)	557, 1221
BsaWI	WCCGGW	1 cut(s)	2253
BsaXI	ACNNNNNCTCC	2 cut(s)	1234, 1264
Bsc4I	CCNNNNNNNGG	4 cut(s)	61, 1046, 1066, 1662
Bse1I	ACTGG	3 cut(s)	1205, 1464, 1860
Bse3DI	GCAATG	3 cut(s)	1114, 1154, 1499
Bse8I	GATNNNNATC	1 cut(s)	2097
BseAI	TCCGGA	1 cut(s)	2253
BseBI	CCWGG	4 cut(s)	762, 813, 945, 1308
BseDI	CCNNGG	2 cut(s)	557, 1221
BseGI	GGATG	9 cut(s)	397, 493, 876, 1117, 1834, 2186, 2315, 2423, 2504
BseJI	GATNNNNATC	1 cut(s)	2097
BseLI	CCNNNNNNNGG	4 cut(s)	61, 1046, 1066, 1662
BseMI	GCAATG	3 cut(s)	1114, 1154, 1499
BseMII	CTCAG	9 cut(s)	123, 224, 504, 558, 599, 947, 1221, 1673, 2373
BseNI	ACTGG	3 cut(s)	1205, 1464, 1860
BseRI	GAGGAG	4 cut(s)	1559, 1623, 1992, 2492
BseSI	GKGCMC	1 cut(s)	902
BseXI	GCAGC	8 cut(s)	57, 415, 616, 742, 935, 1272, 1562, 1814
BseYI	CCCAGC	1 cut(s)	1397
BsgI	GTGCAG	1 cut(s)	741
BshFI	GGCC	2 cut(s)	550, 1306
BshNI	GGYRCC	4 cut(s)	561, 700, 1055, 2239
BsiHKAI	GWGCWC	1 cut(s)	902
BsiHKCI	CYCGRG	2 cut(s)	1696, 2342
BsiSI	CCGG	5 cut(s)	62, 952, 1712, 1938, 2254
BslFI	GGGAC	4 cut(s)	240, 1574, 1700, 2610
BslI	CCNNNNNNNGG	4 cut(s)	61, 1046, 1066, 1662
BsmAI	GTCTC	4 cut(s)	51, 128, 1279, 1781
BsmFI	GGGAC	4 cut(s)	240, 1574, 1700, 2610
BsmI	GAATGC	3 cut(s)	341, 395, 583
BsnI	GGCC	2 cut(s)	550, 1306
Bso31I	GGTCTC	2 cut(s)	51, 128
BsoBI	CYCGRG	2 cut(s)	1696, 2342
Bsp119I	TTCGAA	2 cut(s)	412, 1300
Bsp1286I	GDGCHC	2 cut(s)	380, 902
Bsp13I	TCCGGA	1 cut(s)	2253
Bsp1720I	GCTNAGC	1 cut(s)	933
BspACI	CCGC	7 cut(s)	109, 177, 180, 183, 198, 1240, 1592
BspANI	GGCC	2 cut(s)	550, 1306
BspCNI	CTCAG	9 cut(s)	124, 223, 505, 559, 600, 946, 1222, 1672, 2374
BspEI	TCCGGA	1 cut(s)	2253
BspHI	TCATGA	2 cut(s)	637, 925
BspMI	ACCTGC	1 cut(s)	1199
BspPI	GGATC	7 cut(s)	260, 810, 823, 1511, 1853, 1866, 1919
BspQI	GCTCTTC	1 cut(s)	1896
BspT104I	TTCGAA	2 cut(s)	412, 1300
BspT107I	GGYRCC	4 cut(s)	561, 700, 1055, 2239
BspTI	CTTAAG	1 cut(s)	2177
BspTNI	GGTCTC	2 cut(s)	51, 128
BsrBI	CCGCTC	1 cut(s)	109
BsrDI	GCAATG	3 cut(s)	1114, 1154, 1499
BsrI	ACTGG	3 cut(s)	1205, 1464, 1860
BssECI	CCNNGG	2 cut(s)	557, 1221
BssNI	GRCGYC	1 cut(s)	217
BssSI	CACGAG	1 cut(s)	2321
BssT1I	CCWWGG	1 cut(s)	557
Bst2BI	CACGAG	1 cut(s)	2321
Bst2UI	CCWGG	4 cut(s)	762, 813, 945, 1308
Bst4CI	ACNGT	2 cut(s)	151, 1528
Bst6I	CTCTTC	3 cut(s)	378, 1896, 2102
BstACI	GRCGYC	1 cut(s)	217
BstAFI	CTTAAG	1 cut(s)	2177
BstBAI	YACGTR	2 cut(s)	161, 1331
BstBI	TTCGAA	2 cut(s)	412, 1300
BstC8I	GCNNGC	6 cut(s)	107, 426, 602, 1095, 1653, 2578
BstDEI	CTNAG	9 cut(s)	132, 210, 513, 567, 608, 933, 1230, 1659, 2382
BstDSI	CCRYGG	1 cut(s)	1221
BstEII	GGTNACC	1 cut(s)	1736
BstENI	CCTNNNNNAGG	1 cut(s)	1064
BstF5I	GGATG	9 cut(s)	397, 493, 876, 1117, 1834, 2186, 2315, 2423, 2504
BstMAI	GTCTC	4 cut(s)	51, 128, 1279, 1781
BstMWI	GCNNNNNNNGC	9 cut(s)	183, 189, 204, 547, 1226, 1529, 1652, 1811, 2566
BstNI	CCWGG	4 cut(s)	762, 813, 945, 1308
BstNSI	RCATGY	3 cut(s)	604, 1097, 1405
BstPI	GGTNACC	1 cut(s)	1736
BstSCI	CCNGG	6 cut(s)	60, 760, 811, 943, 951, 1306
BstSFI	CTRYAG	1 cut(s)	1527
BstSLI	GKGCMC	1 cut(s)	902
BstSNI	TACGTA	1 cut(s)	161
BstV1I	GCAGC	8 cut(s)	57, 415, 616, 742, 935, 1272, 1562, 1814
BstV2I	GAAGAC	1 cut(s)	1621
BstX2I	RGATCY	3 cut(s)	25, 815, 1858
BstXI	CCANNNNNNTGG	1 cut(s)	387
BstYI	RGATCY	3 cut(s)	25, 815, 1858
BsuRI	GGCC	2 cut(s)	550, 1306
BtgI	CCRYGG	1 cut(s)	1221
BtrI	CACGTC	1 cut(s)	2457
BtsCI	GGATG	9 cut(s)	397, 493, 876, 1117, 1834, 2186, 2315, 2423, 2504
BtsI	GCAGTG	3 cut(s)	1051, 1964, 2535
BtsIMutI	CAGTG	3 cut(s)	1051, 1964, 2535
BveI	ACCTGC	1 cut(s)	1199
Cac8I	GCNNGC	6 cut(s)	107, 426, 602, 1095, 1653, 2578
CciI	TCATGA	2 cut(s)	637, 925
Cfr13I	GGNCC	2 cut(s)	2462, 2597
CseI	GACGC	3 cut(s)	225, 242, 1448
CsiI	ACCWGGT	1 cut(s)	760
Csp6I	GTAC	9 cut(s)	162, 562, 701, 722, 740, 1056, 1183, 1779, 2645
CspCI	CAANNNNNGTGG	2 cut(s)	1952, 1987
CviQI	GTAC	9 cut(s)	162, 562, 701, 722, 740, 1056, 1183, 1779, 2645
DdeI	CTNAG	9 cut(s)	132, 210, 513, 567, 608, 933, 1230, 1659, 2382
DraI	TTTAAA	1 cut(s)	2002
Eam1104I	CTCTTC	3 cut(s)	378, 1896, 2102
EarI	CTCTTC	3 cut(s)	378, 1896, 2102
Eco105I	TACGTA	1 cut(s)	161
Eco130I	CCWWGG	1 cut(s)	557
Eco24I	GRGCYC	1 cut(s)	380
Eco31I	GGTCTC	2 cut(s)	51, 128
Eco32I	GATATC	2 cut(s)	469, 2259
Eco47I	GGWCC	2 cut(s)	2462, 2597
Eco57I	CTGAAG	1 cut(s)	1791
Eco88I	CYCGRG	2 cut(s)	1696, 2342
Eco91I	GGTNACC	1 cut(s)	1736
EcoNI	CCTNNNNNAGG	1 cut(s)	1064
EcoO109I	RGGNCCY	1 cut(s)	2597
EcoO65I	GGTNACC	1 cut(s)	1736
EcoRII	CCWGG	4 cut(s)	760, 811, 943, 1306
EcoRV	GATATC	2 cut(s)	469, 2259
EcoT14I	CCWWGG	1 cut(s)	557
EcoT22I	ATGCAT	2 cut(s)	397, 1501
EcoT38I	GRGCYC	1 cut(s)	380
ErhI	CCWWGG	1 cut(s)	557
FalI	AAGNNNNNCTT	4 cut(s)	2130, 2162, 2553, 2585
FaqI	GGGAC	4 cut(s)	240, 1574, 1700, 2610
FauI	CCCGC	1 cut(s)	1599
FbaI	TGATCA	1 cut(s)	1597
FblI	GTMKAC	1 cut(s)	962
FokI	GGATG	9 cut(s)	404, 500, 863, 1104, 1841, 2173, 2302, 2430, 2511
FriOI	GRGCYC	1 cut(s)	380
FspBI	CTAG	6 cut(s)	23, 575, 1278, 1568, 1671, 1835
GsaI	CCCAGC	1 cut(s)	1401
HaeIII	GGCC	2 cut(s)	550, 1306
HapII	CCGG	5 cut(s)	62, 952, 1712, 1938, 2254
HgaI	GACGC	3 cut(s)	225, 242, 1448
Hin1I	GRCGYC	1 cut(s)	217
HincII	GTYRAC	4 cut(s)	1509, 1984, 2484, 2632
HindII	GTYRAC	4 cut(s)	1509, 1984, 2484, 2632
HindIII	AAGCTT	2 cut(s)	650, 1420
HinfI	GANTC	9 cut(s)	74, 438, 571, 1201, 1694, 2051, 2305, 2378, 2480
HpaII	CCGG	5 cut(s)	62, 952, 1712, 1938, 2254
HphI	GGTGA	2 cut(s)	542, 2287
Hpy166II	GTNNAC	9 cut(s)	533, 900, 963, 1183, 1509, 1984, 2326, 2484, 2632
Hpy8I	GTNNAC	9 cut(s)	533, 900, 963, 1183, 1509, 1984, 2326, 2484, 2632
Hpy99I	CGWCG	2 cut(s)	219, 2461
HpyCH4III	ACNGT	2 cut(s)	151, 1528
HpyCH4IV	ACGT	6 cut(s)	160, 243, 724, 1330, 2142, 2456
HpyF10VI	GCNNNNNNNGC	9 cut(s)	183, 189, 204, 547, 1226, 1529, 1652, 1811, 2566
HpyF3I	CTNAG	9 cut(s)	132, 210, 513, 567, 608, 933, 1230, 1659, 2382
HpySE526I	ACGT	6 cut(s)	160, 243, 724, 1330, 2142, 2456
Hsp92I	GRCGYC	1 cut(s)	217
Kpn2I	TCCGGA	1 cut(s)	2253
KpnI	GGTACC	3 cut(s)	565, 704, 1059
Ksp22I	TGATCA	1 cut(s)	1597
LguI	GCTCTTC	1 cut(s)	1896
LmnI	GCTCC	5 cut(s)	361, 980, 991, 1075, 1610
Lsp1109I	GCAGC	8 cut(s)	57, 415, 616, 742, 935, 1272, 1562, 1814
LweI	GCATC	8 cut(s)	382, 1486, 2083, 2324, 2344, 2408, 2408, 2489
MabI	ACCWGGT	1 cut(s)	760
MaeI	CTAG	6 cut(s)	23, 575, 1278, 1568, 1671, 1835
MaeII	ACGT	6 cut(s)	160, 243, 724, 1330, 2142, 2456
MaeIII	GTNAC	6 cut(s)	916, 1331, 1736, 1867, 2077, 2533
MbiI	CCGCTC	1 cut(s)	109
MfeI	CAATTG	3 cut(s)	779, 2466, 2541
MflI	RGATCY	3 cut(s)	25, 815, 1858
MhlI	GDGCHC	2 cut(s)	380, 902
MlyI	GAGTC	2 cut(s)	1688, 2489
MmeI	TCCRAC	2 cut(s)	462, 2146
Mph1103I	ATGCAT	2 cut(s)	397, 1501
MroI	TCCGGA	1 cut(s)	2253
MroXI	GAANNNNTTC	3 cut(s)	408, 442, 2055
MseI	TTAA	9 cut(s)	153, 647, 1161, 1314, 1820, 2001, 2178, 2199, 2331
MslI	CAYNNNNRTG	2 cut(s)	1362, 1476
MspA1I	CMGCKG	1 cut(s)	607
MspCI	CTTAAG	1 cut(s)	2177
MspI	CCGG	5 cut(s)	62, 952, 1712, 1938, 2254
MspR9I	CCNGG	6 cut(s)	62, 762, 813, 945, 953, 1308
MunI	CAATTG	3 cut(s)	779, 2466, 2541
Mva1269I	GAATGC	3 cut(s)	341, 395, 583
MvaI	CCWGG	4 cut(s)	762, 813, 945, 1308
MwoI	GCNNNNNNNGC	9 cut(s)	183, 189, 204, 547, 1226, 1529, 1652, 1811, 2566
NciI	CCSGG	2 cut(s)	62, 953
NmuCI	GTSAC	4 cut(s)	916, 1331, 1867, 2533
NsiI	ATGCAT	2 cut(s)	397, 1501
NspI	RCATGY	3 cut(s)	604, 1097, 1405
NspV	TTCGAA	2 cut(s)	412, 1300
PaeI	GCATGC	2 cut(s)	604, 1097
PaeR7I	CTCGAG	2 cut(s)	1696, 2342
PagI	TCATGA	2 cut(s)	637, 925
PciSI	GCTCTTC	1 cut(s)	1896
PcsI	WCGNNNNNNNCGW	2 cut(s)	211, 223
PctI	GAATGC	3 cut(s)	341, 395, 583
PdmI	GAANNNNTTC	3 cut(s)	408, 442, 2055
PfeI	GAWTC	7 cut(s)	74, 438, 571, 1201, 2051, 2305, 2378
PleI	GAGTC	2 cut(s)	1688, 2488
PpsI	GAGTC	2 cut(s)	1688, 2488
Ppu21I	YACGTR	2 cut(s)	161, 1331
PpuMI	RGGWCCY	1 cut(s)	2597
Psp1406I	AACGTT	1 cut(s)	243
Psp5II	RGGWCCY	1 cut(s)	2597
Psp6I	CCWGG	4 cut(s)	760, 811, 943, 1306
PspEI	GGTNACC	1 cut(s)	1736
PspFI	CCCAGC	1 cut(s)	1397
PspGI	CCWGG	4 cut(s)	760, 811, 943, 1306
PspPI	GGNCC	2 cut(s)	2462, 2597
PspPPI	RGGWCCY	1 cut(s)	2597
PsuI	RGATCY	3 cut(s)	25, 815, 1858
PvuII	CAGCTG	1 cut(s)	607
RsaI	GTAC	9 cut(s)	163, 563, 702, 723, 741, 1057, 1184, 1780, 2646
RsaNI	GTAC	9 cut(s)	162, 562, 701, 722, 740, 1056, 1183, 1779, 2645
RseI	CAYNNNNRTG	2 cut(s)	1362, 1476
SapI	GCTCTTC	1 cut(s)	1896
SaqAI	TTAA	9 cut(s)	153, 647, 1161, 1314, 1820, 2001, 2178, 2199, 2331
Sau96I	GGNCC	2 cut(s)	2462, 2597
SchI	GAGTC	2 cut(s)	1688, 2489
ScrFI	CCNGG	6 cut(s)	62, 762, 813, 945, 953, 1308
SduI	GDGCHC	2 cut(s)	380, 902
SexAI	ACCWGGT	1 cut(s)	760
SfaNI	GCATC	8 cut(s)	382, 1486, 2083, 2324, 2344, 2408, 2408, 2489
SfcI	CTRYAG	1 cut(s)	1527
Sfr274I	CTCGAG	2 cut(s)	1696, 2342
SfuI	TTCGAA	2 cut(s)	412, 1300
SinI	GGWCC	2 cut(s)	2462, 2597
SlaI	CTCGAG	2 cut(s)	1696, 2342
SmiMI	CAYNNNNRTG	2 cut(s)	1362, 1476
SmlI	CTYRAG	6 cut(s)	626, 785, 1696, 2147, 2177, 2342
SmoI	CTYRAG	6 cut(s)	626, 785, 1696, 2147, 2177, 2342
SnaBI	TACGTA	1 cut(s)	161
SphI	GCATGC	2 cut(s)	604, 1097
SsiI	CCGC	7 cut(s)	109, 177, 180, 183, 198, 1240, 1592
SspMI	CTAG	6 cut(s)	23, 575, 1278, 1568, 1671, 1835
StyD4I	CCNGG	6 cut(s)	60, 760, 811, 943, 951, 1306
StyI	CCWWGG	1 cut(s)	557
TaaI	ACNGT	2 cut(s)	151, 1528
TaiI	ACGT	6 cut(s)	163, 246, 727, 1333, 2145, 2459
TaqI	TCGA	6 cut(s)	345, 412, 446, 1300, 1697, 2343
TaqII	GACCGA	1 cut(s)	2122
TatI	WGTACW	2 cut(s)	739, 1182
TauI	GCSGC	3 cut(s)	180, 183, 186
TfiI	GAWTC	7 cut(s)	74, 438, 571, 1201, 2051, 2305, 2378
Tru1I	TTAA	9 cut(s)	153, 647, 1161, 1314, 1820, 2001, 2178, 2199, 2331
Tru9I	TTAA	9 cut(s)	153, 647, 1161, 1314, 1820, 2001, 2178, 2199, 2331
TscAI	CASTG	3 cut(s)	1051, 1964, 2542
TseFI	GTSAC	4 cut(s)	916, 1331, 1867, 2533
TseI	GCWGC	8 cut(s)	45, 428, 604, 755, 948, 1285, 1575, 1802
Tsp45I	GTSAC	4 cut(s)	916, 1331, 1867, 2533
TspGWI	ACGGA	3 cut(s)	215, 1620, 2152
TspRI	CASTG	3 cut(s)	1051, 1964, 2542
Vha464I	CTTAAG	1 cut(s)	2177
VneI	GTGCAC	1 cut(s)	898
VpaK11BI	GGWCC	2 cut(s)	2462, 2597
XagI	CCTNNNNNAGG	1 cut(s)	1064
XapI	RAATTY	4 cut(s)	589, 1128, 2112, 2155
XceI	RCATGY	3 cut(s)	604, 1097, 1405
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1748
XhoI	CTCGAG	2 cut(s)	1696, 2342
XmiI	GTMKAC	1 cut(s)	962
XmnI	GAANNNNTTC	3 cut(s)	408, 442, 2055
XspI	CTAG	6 cut(s)	23, 575, 1278, 1568, 1671, 1835
Zsp2I	ATGCAT	2 cut(s)	397, 1501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.