Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009254

Physical Location & Seq

Genomic Coordinates Reverse (-)

18543893 .. 18547556

Gene Structure 3664 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G40000.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGGAAGAGGGAGACGACACAGAGGTTCAACTTCGAAGAGAAATCAAATCGAAAATAGCAACGACGACAATGTTCGAAAACCCCTCAAATCCGAAGCTTCCTTTGATTCCAACAACTTGCAAAAGCCCATCTGCTCAAATCTTCATTTTGAGGAGTATTACAAGGAACAAAGAATAGTGAAAGCTCAAGAGTGGGATTCGTTTATGGAGTTTCTTCGTAAGCCATTACCTGCTGCTTTCAGAGTTTATTCCAATGGTCAATTTTGTGATGAGATTCGACTGAAATTGGAGAATGATTTCATGATATCTCTTCAGGCTGAGGCTATAGAGAGTGGTGAATTGGAGGCTATCAAGCCTCTGCCTTGGTATCCTAAGAATCTTGCTTGGCATTCTAATTTTTCACGCAAGGAGATTAGGAAAAACCAGACACTTGAGAGGTTTCATGAGTTTCTTAAGTTAGAAACTGAAGCTGGGAACATGACCAGGCAAGAATCAGTTAGCATGGTACCTCCTCTCTTCCTTGATGTACATCCAGATCATTTTGTTCTCGACATGTGTGCTGCACCTGGTTCCAAGACATTTCAGCTGCTTGAAATAATACATGAATCATCAGAACCAGGATCTCTACCTAATGGAATGGTGGTGGCGAATGATGTTGATTATAAAAGATCTAATCTTCTTATTCACCAAACGAAAAGAACATGTACGACCAATTTGATGGTCACTAACAACGAAGGTCAACATTTCCCCAGTTGCAACACCAAGAGAACCTTGTCTGTAGCATCTGAGACTAATCCTCATCCCATCGATCAGCTTCTTTTTGATCGTGTCTTATGTGATGTCCCGTGTAGTGGTGATGGTACTCTGCGCAAGGCTCCAGACATATGGCGAAGATGGAATTCTGGATCTGGCAATGGACTTCATAGTCTACAGGTTGTGCTTGCCATGAGAGGTTTATCTCTGCTAAAAGTTGGTGGGAGAATGGTATACTCAACCTGCTCAATGAACCCAATTGAAGATGAAGCTGTTGTTGCTGAAATTCTGAGGAGATGTGGATGCTCTGTTGAGCTTGTAGATGTCTCAGATAAGCTTCCAGAACTTATTAGAAGACCAGGTCTTACGAAATGGAAGGTGCATGACAGAGGTGGCTGGTATAGATCTTACAAAGATGTTCCAAAGTCACAGAGAGACGGAGTTCTTAGGAGCATGTTTCCTTCTGGTAAAAGTGATAAAGACTCATCGGGTGGCAAAAACAGCTATGAAGAAATGGCTTCTATCTCTTCTGATGAATCAGCCGAAGAAGTCTGTGATCTTCCCCTTGAACTTTGTATGAGGATACTGCCTCATGATCAAAACACTGGGGGATTTTTCATTGCAGTCCTTCACAAAGTTTCACCTTTACCAGACTTTCAGGAGAAAGTAACTCAGAAAAGGAATTTGTCTACTAGAAACAACATCAACTCATCTGAGAAGTCTTCTTCGTATGAAGCAGTATCTGACACTGTGGTTACTAAACCTGAAGAGGGTACAGAGGAAATAGTTTTAGAAGCAACAGTTTCAGAGAATGGCTTTGAGCCGCCGGAGATGGAAAGCACCTGGAATGAAGGTATCGTAGAATTGGCTCAAAAACTTGATGATATAGAAGGCAAGAGAGAAGTACCATCAATGCAAGGGAAGTGGAAAGGCCTTTACCCTGTTGTTTTCTTGAGAGATCAAACAGGGATCAACGGGATCAAGACATTTTACGGCATTAAAGATGAATCGTTTCCGCTGTATGGTCATCTCGTGACTAGAAACAGCGACATAAGCAGCCACGGAAACGTGAAAAGGATTTACTATGTTTCGAAAGCAGTTAAGAATGTTCTTGAGCTTAATTTTGCAGTTGGGAAGCCGCTCAAGATCTCCTCTGTTGGCCTCAAGATGTTTGAGAAACAATCTGCAAAAGAATGTGAAGCCAACTGTTGCTCATTCCGAATAACGTCAGAGGGATTGCCTGTGATCCTTCCGTACATGACCAAGCAAATACTTTATGCAACAATGGCGGATTTCAAGAATCTCCTGCAACACAAATCAATCAAGTTTCTAGATTTCATACATCCACAGATTGGTGAGAAAGCAGCAGAACTAGCCTTGGGATCTTGCGTTATGGTTCTCGTTGATGATTCTCAGCTCGGGTCAGAACAATTCAAAGTGAATTCATCAACAATAGCCATTGGATGCTGGAAGGGCAAGGCTAGCTTAACCGTTATGGTCACAACAGTCGATTGTCACCAACTTATCCAGAGGCTTTCTGATAGAAGTAAAGAAAAAGAAAAAGCTGAGAACTGTTTCAAGACTGCTGAGACAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016427 GO:0016428 GO:0016740 GO:0016741 GO:0030488 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltr_RsmB-F

Protein Analysis

783

Amino Acids

88.18

Weight (kDa)

6.48

Isoelectric Point (pI)

43.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltr_RsmB-F	PF01189	2 - 139	4.6e-23	16S rRNA methyltransferase RsmB/F
Pre-PUA_NSUN2	PF25376	353 - 460	3.8e-29	NSUN2 Pre-PUA domain
PUA_NSUN2	PF25378	462 - 552	3.2e-22	NSUN2 PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22400 AT4G40000 AT4G40000
fragaria_vesca	FvH4_1g20370 FvH4_1g20370 FvH4_1g20370
malus_domestica	MD01G1034300.v1.1 MD15G1321600.v1.1
prunus_persica	Prupe.6G191700_v2.0.a1 Prupe.6G191700_v2.0.a1
pyrus_communis	pycom01g06140 pycom15g28220
rosa_chinensis	RchiOBHm_Chr2g0112501
rosa_laevigata	RLG00000017986
rosa_multiflora	Rmu_sc0001499.1_g000061
rosa_roxburghii	Rroxscaffold_2G00131370
rosa_rugosa	Rorug02G0181300
rosa_samantha	Rh2AG234500 Rh2BG248100 Rh2CG239100 Rh2DG242100
rosa_wichuraiana	Rw2G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	663
AasI	GACNNNNNNGTC	1 cut(s)	924
Acc16I	TGCGCA	1 cut(s)	869
Acc36I	ACCTGC	2 cut(s)	238, 1004
Acc65I	GGTACC	1 cut(s)	505
AccB1I	GGYRCC	1 cut(s)	505
AccB7I	CCANNNNNTGG	1 cut(s)	2105
AccBSI	CCGCTC	1 cut(s)	1894
AccI	GTMKAC	3 cut(s)	928, 987, 1442
AciI	CCGC	4 cut(s)	1577, 1769, 1892, 2042
AclWI	GGATC	6 cut(s)	628, 913, 1730, 1739, 1993, 2143
AcsI	RAATTY	4 cut(s)	898, 1038, 1435, 2194
AcuI	CTGAAG	3 cut(s)	296, 486, 1539
AfaI	GTAC	7 cut(s)	507, 528, 706, 862, 1528, 1659, 2009
AfiI	CCNNNNNNNGG	4 cut(s)	851, 1523, 1775, 2105
AflII	CTTAAG	1 cut(s)	452
AflIII	ACRYGT	2 cut(s)	552, 701
AgsI	TTSAA	7 cut(s)	30, 593, 1016, 1322, 2050, 2188, 2332
AjnI	CCWGG	5 cut(s)	482, 565, 616, 1111, 1595
Alw26I	GTCTC	5 cut(s)	7, 782, 1084, 1182, 2336
AlwI	GGATC	6 cut(s)	628, 913, 1730, 1739, 1993, 2143
AlwNI	CAGNNNCTG	1 cut(s)	1496
Ama87I	CYCGRG	1 cut(s)	2171
AoxI	GGCC	2 cut(s)	1684, 1912
ApeKI	GCWGC	5 cut(s)	233, 560, 586, 1809, 2117
ApoI	RAATTY	4 cut(s)	898, 1038, 1435, 2194
Asp700I	GAANNNNTTC	3 cut(s)	296, 1764, 2327
Asp718I	GGTACC	1 cut(s)	505
AspLEI	GCGC	1 cut(s)	870
AsuHPI	GGTGA	6 cut(s)	347, 677, 866, 1386, 2120, 2261
AsuII	TTCGAA	3 cut(s)	35, 76, 1844
AsuNHI	GCTAGC	1 cut(s)	2234
AvaI	CYCGRG	1 cut(s)	2171
BanI	GGYRCC	1 cut(s)	505
BauI	CACGAG	1 cut(s)	1784
BbsI	GAAGAC	2 cut(s)	1114, 1467
BbvCI	CCTCAGC	1 cut(s)	318
BbvI	GCAGC	5 cut(s)	220, 547, 573, 1821, 2129
BccI	CCATC	7 cut(s)	137, 712, 812, 851, 888, 1579, 1669
BceAI	ACGGC	1 cut(s)	1762
BcgI	CGANNNNNNTGC	2 cut(s)	539, 573
BciT130I	CCWGG	5 cut(s)	484, 567, 618, 1113, 1597
BciVI	GTATCC	2 cut(s)	378, 1329
BclI	TGATCA	1 cut(s)	1348
BcoDI	GTCTC	5 cut(s)	7, 782, 1084, 1182, 2336
BfaI	CTAG	5 cut(s)	1446, 1791, 2084, 2126, 2235
BfmI	CTRYAG	3 cut(s)	324, 777, 929
BfrI	CTTAAG	1 cut(s)	452
BfuAI	ACCTGC	2 cut(s)	238, 1004
BfuI	GTATCC	2 cut(s)	378, 1329
BglII	AGATCT	3 cut(s)	668, 1157, 1899
BisI	GCNGC	7 cut(s)	234, 561, 587, 1577, 1810, 1892, 2118
BlsI	GCNGC	7 cut(s)	235, 562, 588, 1578, 1811, 1893, 2119
Bme1390I	CCNGG	5 cut(s)	484, 567, 618, 1113, 1597
BmeT110I	CYCGRG	1 cut(s)	2171
BmiI	GGNNCC	3 cut(s)	507, 571, 876
BmrFI	CCNGG	5 cut(s)	484, 567, 618, 1113, 1597
BmrI	ACTGGG	2 cut(s)	744, 1368
BmsI	GCATC	3 cut(s)	791, 1046, 2207
BmtI	GCTAGC	1 cut(s)	2238
BmuI	ACTGGG	2 cut(s)	744, 1368
BpiI	GAAGAC	2 cut(s)	1114, 1467
BpmI	CTGGAG	1 cut(s)	861
Bpu10I	CCTNAGC	1 cut(s)	318
Bpu14I	TTCGAA	3 cut(s)	35, 76, 1844
BpuEI	CTTGAG	6 cut(s)	171, 452, 1726, 1880, 1886, 1901
Bsa29I	ATCGAT	1 cut(s)	807
BsaBI	GATNNNNATC	1 cut(s)	528
BsaJI	CCNNGG	3 cut(s)	362, 1813, 2130
Bsc4I	CCNNNNNNNGG	4 cut(s)	851, 1523, 1775, 2105
Bse1I	ACTGG	2 cut(s)	750, 1363
Bse3DI	GCAATG	2 cut(s)	919, 1371
Bse8I	GATNNNNATC	1 cut(s)	528
BseBI	CCWGG	5 cut(s)	484, 567, 618, 1113, 1597
BseCI	ATCGAT	1 cut(s)	807
BseDI	CCNNGG	3 cut(s)	362, 1813, 2130
BseGI	GGATG	5 cut(s)	529, 799, 1061, 2095, 2222
BseJI	GATNNNNATC	1 cut(s)	528
BseLI	CCNNNNNNNGG	4 cut(s)	851, 1523, 1775, 2105
BseMI	GCAATG	2 cut(s)	919, 1371
BseMII	CTCAG	9 cut(s)	309, 777, 1034, 1095, 1439, 1458, 2180, 2310, 2331
BseNI	ACTGG	2 cut(s)	750, 1363
BseRI	GAGGAG	4 cut(s)	167, 501, 1060, 1894
BseXI	GCAGC	5 cut(s)	220, 547, 573, 1821, 2129
BseYI	CCCAGC	1 cut(s)	470
BsgI	GTGCAG	1 cut(s)	546
BshFI	GGCC	2 cut(s)	1686, 1914
BshNI	GGYRCC	1 cut(s)	505
BshVI	ATCGAT	1 cut(s)	807
BsiHKCI	CYCGRG	1 cut(s)	2171
BsiSI	CCGG	1 cut(s)	1580
BslFI	GGGAC	1 cut(s)	827
BslI	CCNNNNNNNGG	4 cut(s)	851, 1523, 1775, 2105
BsmAI	GTCTC	5 cut(s)	7, 782, 1084, 1182, 2336
BsmBI	CGTCTC	2 cut(s)	7, 1182
BsmFI	GGGAC	1 cut(s)	827
BsmI	GAATGC	1 cut(s)	388
BsnI	GGCC	2 cut(s)	1686, 1914
BsoBI	CYCGRG	1 cut(s)	2171
Bsp119I	TTCGAA	3 cut(s)	35, 76, 1844
Bsp1407I	TGTACA	1 cut(s)	526
BspACI	CCGC	4 cut(s)	1577, 1769, 1892, 2042
BspANI	GGCC	2 cut(s)	1686, 1914
BspCNI	CTCAG	9 cut(s)	310, 778, 1035, 1094, 1438, 1459, 2179, 2311, 2332
BspDI	ATCGAT	1 cut(s)	807
BspHI	TCATGA	3 cut(s)	300, 442, 1345
BspLI	GGNNCC	3 cut(s)	507, 571, 876
BspMI	ACCTGC	2 cut(s)	238, 1004
BspOI	GCTAGC	1 cut(s)	2238
BspPI	GGATC	6 cut(s)	628, 913, 1730, 1739, 1993, 2143
BspT104I	TTCGAA	3 cut(s)	35, 76, 1844
BspT107I	GGYRCC	1 cut(s)	505
BspTI	CTTAAG	1 cut(s)	452
BsrBI	CCGCTC	1 cut(s)	1894
BsrDI	GCAATG	2 cut(s)	919, 1371
BsrGI	TGTACA	1 cut(s)	526
BsrI	ACTGG	2 cut(s)	750, 1363
BssECI	CCNNGG	3 cut(s)	362, 1813, 2130
BssNAI	GTATAC	1 cut(s)	988
BssSI	CACGAG	1 cut(s)	1784
BssT1I	CCWWGG	2 cut(s)	362, 2130
Bst1107I	GTATAC	1 cut(s)	988
Bst2BI	CACGAG	1 cut(s)	1784
Bst2UI	CCWGG	5 cut(s)	484, 567, 618, 1113, 1597
Bst4CI	ACNGT	6 cut(s)	1504, 1555, 1961, 2245, 2260, 2327
Bst6I	CTCTTC	5 cut(s)	32, 315, 521, 1285, 1515
BstAFI	CTTAAG	1 cut(s)	452
BstAUI	TGTACA	1 cut(s)	526
BstBI	TTCGAA	3 cut(s)	35, 76, 1844
BstC8I	GCNNGC	2 cut(s)	942, 2236
BstDSI	CCRYGG	1 cut(s)	1813
BstF5I	GGATG	5 cut(s)	529, 799, 1061, 2095, 2222
BstHHI	GCGC	1 cut(s)	870
BstMAI	GTCTC	5 cut(s)	7, 782, 1084, 1182, 2336
BstMWI	GCNNNNNNNGC	8 cut(s)	1031, 1254, 1806, 2039, 2126, 2226, 2235, 2345
BstNI	CCWGG	5 cut(s)	484, 567, 618, 1113, 1597
BstNSI	RCATGY	3 cut(s)	556, 705, 1210
BstSCI	CCNGG	5 cut(s)	482, 565, 616, 1111, 1595
BstSFI	CTRYAG	3 cut(s)	324, 777, 929
BstV1I	GCAGC	5 cut(s)	220, 547, 573, 1821, 2129
BstV2I	GAAGAC	2 cut(s)	1114, 1467
BstX2I	RGATCY	6 cut(s)	620, 668, 905, 1157, 1899, 2135
BstXI	CCANNNNNNTGG	2 cut(s)	718, 885
BstYI	RGATCY	6 cut(s)	620, 668, 905, 1157, 1899, 2135
BstZ17I	GTATAC	1 cut(s)	988
Bsu15I	ATCGAT	1 cut(s)	807
BsuI	GTATCC	2 cut(s)	378, 1329
BsuRI	GGCC	2 cut(s)	1686, 1914
BsuTUI	ATCGAT	1 cut(s)	807
BtgI	CCRYGG	1 cut(s)	1813
BtsCI	GGATG	5 cut(s)	529, 799, 1061, 2095, 2222
BtsIMutI	CAGTG	2 cut(s)	1356, 1500
BveI	ACCTGC	2 cut(s)	238, 1004
Cac8I	GCNNGC	2 cut(s)	942, 2236
CaiI	CAGNNNCTG	1 cut(s)	1496
CciI	TCATGA	3 cut(s)	300, 442, 1345
CfoI	GCGC	1 cut(s)	870
ClaI	ATCGAT	1 cut(s)	807
CsiI	ACCWGGT	2 cut(s)	565, 1111
Csp6I	GTAC	7 cut(s)	506, 527, 705, 861, 1527, 1658, 2008
CspCI	CAANNNNNGTGG	2 cut(s)	1658, 1693
CviQI	GTAC	7 cut(s)	506, 527, 705, 861, 1527, 1658, 2008
DrdI	GACNNNNNNGTC	1 cut(s)	924
DseDI	GACNNNNNNGTC	1 cut(s)	924
Eam1104I	CTCTTC	5 cut(s)	32, 315, 521, 1285, 1515
EarI	CTCTTC	5 cut(s)	32, 315, 521, 1285, 1515
EciI	GGCGGA	1 cut(s)	2057
Eco130I	CCWWGG	2 cut(s)	362, 2130
Eco147I	AGGCCT	1 cut(s)	1686
Eco32I	GATATC	1 cut(s)	306
Eco57I	CTGAAG	3 cut(s)	296, 486, 1539
Eco88I	CYCGRG	1 cut(s)	2171
EcoRI	GAATTC	2 cut(s)	898, 2194
EcoRII	CCWGG	5 cut(s)	482, 565, 616, 1111, 1595
EcoRV	GATATC	1 cut(s)	306
EcoT14I	CCWWGG	2 cut(s)	362, 2130
ErhI	CCWWGG	2 cut(s)	362, 2130
Esp3I	CGTCTC	2 cut(s)	7, 1182
FalI	AAGNNNNNCTT	4 cut(s)	755, 787, 2222, 2254
FaqI	GGGAC	1 cut(s)	827
FauNDI	CATATG	1 cut(s)	884
FbaI	TGATCA	1 cut(s)	1348
FblI	GTMKAC	3 cut(s)	928, 987, 1442
Fnu4HI	GCNGC	7 cut(s)	234, 561, 587, 1577, 1810, 1892, 2118
FokI	GGATG	5 cut(s)	516, 786, 1068, 2082, 2229
Fsp4HI	GCNGC	7 cut(s)	234, 561, 587, 1577, 1810, 1892, 2118
FspBI	CTAG	5 cut(s)	1446, 1791, 2084, 2126, 2235
FspI	TGCGCA	1 cut(s)	869
GlaI	GCGC	1 cut(s)	869
GluI	GCNGC	7 cut(s)	234, 561, 587, 1577, 1810, 1892, 2118
GsaI	CCCAGC	1 cut(s)	474
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	2 cut(s)	1686, 1914
HapII	CCGG	1 cut(s)	1580
HhaI	GCGC	1 cut(s)	870
Hin6I	GCGC	1 cut(s)	868
HinP1I	GCGC	1 cut(s)	868
HincII	GTYRAC	1 cut(s)	740
HindII	GTYRAC	1 cut(s)	740
HindIII	AAGCTT	2 cut(s)	96, 1088
HpaII	CCGG	1 cut(s)	1580
HphI	GGTGA	6 cut(s)	347, 677, 866, 1386, 2120, 2261
Hpy166II	GTNNAC	4 cut(s)	740, 929, 988, 1443
Hpy8I	GTNNAC	4 cut(s)	740, 929, 988, 1443
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	8 cut(s)	728, 1123, 1224, 1391, 1598, 1637, 2012, 2218
HpyCH4III	ACNGT	6 cut(s)	1504, 1555, 1961, 2245, 2260, 2327
HpyCH4IV	ACGT	2 cut(s)	1821, 1979
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1031, 1254, 1806, 2039, 2126, 2226, 2235, 2345
HpySE526I	ACGT	2 cut(s)	1821, 1979
HspAI	GCGC	1 cut(s)	868
KpnI	GGTACC	1 cut(s)	509
Ksp22I	TGATCA	1 cut(s)	1348
LmnI	GCTCC	2 cut(s)	880, 1203
Lsp1109I	GCAGC	5 cut(s)	220, 547, 573, 1821, 2129
LweI	GCATC	3 cut(s)	791, 1046, 2207
MabI	ACCWGGT	2 cut(s)	565, 1111
MaeI	CTAG	5 cut(s)	1446, 1791, 2084, 2126, 2235
MaeII	ACGT	2 cut(s)	1821, 1979
MaeIII	GTNAC	7 cut(s)	721, 1179, 1420, 1507, 1786, 2251, 2267
MbiI	CCGCTC	1 cut(s)	1894
MfeI	CAATTG	1 cut(s)	1011
MflI	RGATCY	6 cut(s)	620, 668, 905, 1157, 1899, 2135
MlyI	GAGTC	1 cut(s)	1229
MmeI	TCCRAC	1 cut(s)	135
MroXI	GAANNNNTTC	3 cut(s)	296, 1764, 2327
MseI	TTAA	5 cut(s)	453, 1752, 1854, 1872, 2240
MspA1I	CMGCKG	2 cut(s)	586, 1771
MspCI	CTTAAG	1 cut(s)	452
MspI	CCGG	1 cut(s)	1580
MspR9I	CCNGG	5 cut(s)	484, 567, 618, 1113, 1597
MunI	CAATTG	1 cut(s)	1011
Mva1269I	GAATGC	1 cut(s)	388
MvaI	CCWGG	5 cut(s)	484, 567, 618, 1113, 1597
MwoI	GCNNNNNNNGC	8 cut(s)	1031, 1254, 1806, 2039, 2126, 2226, 2235, 2345
NdeI	CATATG	1 cut(s)	884
NheI	GCTAGC	1 cut(s)	2234
NlaIV	GGNNCC	3 cut(s)	507, 571, 876
NmuCI	GTSAC	5 cut(s)	721, 1179, 1786, 2251, 2267
NsbI	TGCGCA	1 cut(s)	869
NspI	RCATGY	3 cut(s)	556, 705, 1210
NspV	TTCGAA	3 cut(s)	35, 76, 1844
PagI	TCATGA	3 cut(s)	300, 442, 1345
PceI	AGGCCT	1 cut(s)	1686
PciI	ACATGT	2 cut(s)	552, 701
PctI	GAATGC	1 cut(s)	388
PdmI	GAANNNNTTC	3 cut(s)	296, 1764, 2327
PflFI	GACNNNGTC	1 cut(s)	1113
PflMI	CCANNNNNTGG	1 cut(s)	2105
PkrI	GCNGC	7 cut(s)	235, 562, 588, 1578, 1811, 1893, 2119
PleI	GAGTC	1 cut(s)	1229
PpsI	GAGTC	1 cut(s)	1229
PscI	ACATGT	2 cut(s)	552, 701
PsiI	TTATAA	1 cut(s)	663
Psp6I	CCWGG	5 cut(s)	482, 565, 616, 1111, 1595
PspFI	CCCAGC	1 cut(s)	470
PspGI	CCWGG	5 cut(s)	482, 565, 616, 1111, 1595
PspN4I	GGNNCC	3 cut(s)	507, 571, 876
PstNI	CAGNNNCTG	1 cut(s)	1496
PsuI	RGATCY	6 cut(s)	620, 668, 905, 1157, 1899, 2135
PsyI	GACNNNGTC	1 cut(s)	1113
PvuII	CAGCTG	1 cut(s)	586
RsaI	GTAC	7 cut(s)	507, 528, 706, 862, 1528, 1659, 2009
RsaNI	GTAC	7 cut(s)	506, 527, 705, 861, 1527, 1658, 2008
SaqAI	TTAA	5 cut(s)	453, 1752, 1854, 1872, 2240
SatI	GCNGC	7 cut(s)	234, 561, 587, 1577, 1810, 1892, 2118
SchI	GAGTC	1 cut(s)	1229
ScrFI	CCNGG	5 cut(s)	484, 567, 618, 1113, 1597
SexAI	ACCWGGT	2 cut(s)	565, 1111
SfaNI	GCATC	3 cut(s)	791, 1046, 2207
SfcI	CTRYAG	3 cut(s)	324, 777, 929
SfuI	TTCGAA	3 cut(s)	35, 76, 1844
SmlI	CTYRAG	7 cut(s)	186, 431, 452, 1705, 1865, 1895, 1916
SmoI	CTYRAG	7 cut(s)	186, 431, 452, 1705, 1865, 1895, 1916
SseBI	AGGCCT	1 cut(s)	1686
SsiI	CCGC	4 cut(s)	1577, 1769, 1892, 2042
SspMI	CTAG	5 cut(s)	1446, 1791, 2084, 2126, 2235
StuI	AGGCCT	1 cut(s)	1686
StyD4I	CCNGG	5 cut(s)	482, 565, 616, 1111, 1595
StyI	CCWWGG	2 cut(s)	362, 2130
TaaI	ACNGT	6 cut(s)	1504, 1555, 1961, 2245, 2260, 2327
TaiI	ACGT	2 cut(s)	1824, 1982
TaqI	TCGA	8 cut(s)	35, 51, 76, 277, 549, 807, 1844, 2262
TatI	WGTACW	1 cut(s)	526
TauI	GCSGC	2 cut(s)	1579, 1894
Tru1I	TTAA	5 cut(s)	453, 1752, 1854, 1872, 2240
Tru9I	TTAA	5 cut(s)	453, 1752, 1854, 1872, 2240
TscAI	CASTG	2 cut(s)	1363, 1507
TseFI	GTSAC	5 cut(s)	721, 1179, 1786, 2251, 2267
TseI	GCWGC	5 cut(s)	233, 560, 586, 1809, 2117
Tsp45I	GTSAC	5 cut(s)	721, 1179, 1786, 2251, 2267
TspGWI	ACGGA	3 cut(s)	1206, 1830, 1995
TspRI	CASTG	2 cut(s)	1363, 1507
Tth111I	GACNNNGTC	1 cut(s)	1113
Van91I	CCANNNNNTGG	1 cut(s)	2105
Vha464I	CTTAAG	1 cut(s)	452
XapI	RAATTY	4 cut(s)	898, 1038, 1435, 2194
XbaI	TCTAGA	1 cut(s)	2083
XceI	RCATGY	3 cut(s)	556, 705, 1210
XmiI	GTMKAC	3 cut(s)	928, 987, 1442
XmnI	GAANNNNTTC	3 cut(s)	296, 1764, 2327
XspI	CTAG	5 cut(s)	1446, 1791, 2084, 2126, 2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.