Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009254

Physical Location & Seq

Genomic Coordinates Forward (+)

33673342 .. 33678375

Gene Structure 5034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1321600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2043 bp

ATGCATAATTCCTTGAGGGACTTCTTTTGTTGGGAACTCTCTGTTCTGTGGTATATGTGCGCTGCACCAGGTTCGAAAACATTCCAATTGCTTGAGGTAATACACCGATCAATGAAACCAGGATCTCTACCTGATGGATTGGTTGTAGCAAATGATCTTGATGTCCAAAGATGTAATCTACTCATCCATCAAACAAAAAGAATGTGCACAGCCAACTTGATAGTCACAAATCATGAAGCTCAGCACTTTCCTGGTTGCCGGGCAAAGTCTAGTGCTTCTGAAATAGGAGCTGAAATGGAGCCACCTATCAGCCATCTTATCTTTGATCGTGTTTTATGTGATGTCCCTTGCAGTGGAGACGGTACCCTTCGCAAGGCTCCTGATATCTGGAGGAAATGGTATAACTTTAATTTTTCAGCATGCTGGACTGGGAAATGGGGTTCATCCTCTACAAATTTAGATAGCTATGCGAGTTTATTCTCATATGTTATATTGTTTATCCTAGGTTTATTGCTGCTTAAAGTTGGCGGAAGAATGGTGTACTCTACCTGCTCTATGAATCCAGTTGAAAATGAAGCTGTGGTTGCTGAGATTTTGCGGAGAGGTGGTGGGTCTGTTGAGCTTATTGATGTCTCTAGTGAGCTTCCCCATCTTGTTCGGAGGCTAGGTCTTAAAAAATGGAAGATACATGACAAGGGTAGGTGGTTACCTTCCCACAAAAATGTCAGCAAATATCGGAAAAATATCATTGTTCCCAGCATGTTTCCTTCTGGAAGAAGCTTTACAGAACCAACAGACGGTAATGATAATATGGAAGTGGAAGAAAAGCATGAGAATGGTGGCAATGGAAATTTTGAAGATGCATTGCAGTCAACTGATGATCCTGCAACTGTAGCCAACGAACAAGAGGAGGAAGTTTCTGAATTTCCACTAGAACGCTGCATGAGGATAGTCCCGCATGATCAAAATGGAGGAGCTTTCTTCATTGCTGTCTTCCACAAACATTCTGCTTTGCCAGCCAATCAGGGGAAACCTAATGGTCTGAAAGAGGACTTGGGCACTAGAAATGACGAGCCGCAGGTAGAATTACAAAATCTGGGTACTGAAGGTACAAATGGGACGGTGGCTAGTTTGGAAGATGGTACAGATGAGACTTTTTTAGAAGCAGCTTCAGAGGTAGAGTTAATAAAGGAAGAACTAGATGGGGCATCTTTGGAGCTTGATCCTTCTGTCACGCTTGAAGAAGATGTATCGGAGGAAGCCCAGGAGCCTGTTGATGAGGAAACAGATCCCAAGAAAGCTGGAGGAAAGAGAAAGAAGCAGTGTCAAGGCAAGTGGAGAGGAGTGGACCCTGTTGTTTTCTTTAAAGATGAAGCTACCATTAATAGCATAAAGACATTCTATGGCATTGATGAATCGTTTCCCTTCAATGACCACCTTGTCACAAGAAACACCGATGCTAATCATGTGAAAAGAATTTACTACATCTCAAAGTCAGTCAAAGATGTTCTTGAGCTGAATTTCTCCGTTGGACAGCAACTTAAGATAACATCTATTGGGCTTAAGATGTTCGAACGACAAACTGCAAGAGAAGGAAATTTGGCGCCTTGTTTATTCCGGATATCACCAGAAGGATTGCCACTTATTCTTCCGTACATAACCAAACAGATTCTATGTGCATCCCTAGTCGACTTTAAACATCTTCTTGAGTATAAATCTATCAAGTTTGCTGATTTTGTTGATTCTGCGTTCAGTCAGAAGGCATCAGGCATAATGTCAGGATGCTGTGTGATAGTTTTGAGTAAAGATGGCGAAGCCACGTCGGGTCCTATTGAAGTCGACGAGTCAACAATAGCCATTGGATGCTGGAAAGGTAGGTCCAGTTTGTCTGTGATGATCACAGCAATTGACTGCCAAGAACTGCAAGAAAGGCTTTTGGCGCGCATGGAAACTGAAAAGGAGCCTTCGGAGAAGAAAGACGAGGCTTCTAGTGTCAACAGGGATGAGGTACAGGACGTAAAGGATATTGAAAAGGAACGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltr_RsmB-F

Protein Analysis

681

Amino Acids

75.83

Weight (kDa)

5.13

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltr_RsmB-F	PF01189	18 - 210	5.5e-25	16S rRNA methyltransferase RsmB/F
Pre-PUA_NSUN2	PF25376	451 - 554	3e-34	NSUN2 Pre-PUA domain
PUA_NSUN2	PF25378	556 - 646	8.3e-26	NSUN2 PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22400 AT4G40000 AT4G40000
fragaria_vesca	FvH4_1g20370 FvH4_1g20370 FvH4_1g20370
malus_domestica	MD01G1034300.v1.1 MD15G1321600.v1.1
prunus_persica	Prupe.6G191700_v2.0.a1 Prupe.6G191700_v2.0.a1
pyrus_communis	pycom01g06140 pycom15g28220
rosa_chinensis	RchiOBHm_Chr2g0112501
rosa_laevigata	RLG00000017986
rosa_multiflora	Rmu_sc0001499.1_g000061
rosa_roxburghii	Rroxscaffold_2G00131370
rosa_rugosa	Rorug02G0181300
rosa_samantha	Rh2AG234500 Rh2BG248100 Rh2CG239100 Rh2DG242100
rosa_wichuraiana	Rw2G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1439
Acc36I	ACCTGC	2 cut(s)	557, 1069
Acc65I	GGTACC	1 cut(s)	362
AccB1I	GGYRCC	2 cut(s)	362, 1603
AccI	GTMKAC	2 cut(s)	1689, 1839
AccII	CGCG	1 cut(s)	1942
AccIII	TCCGGA	1 cut(s)	1617
AciI	CCGC	4 cut(s)	528, 598, 956, 1076
AclI	AACGTT	1 cut(s)	2038
AclWI	GGATC	4 cut(s)	130, 875, 1217, 1283
AcsI	RAATTY	6 cut(s)	454, 850, 923, 1476, 1519, 1597
AcuI	CTGAAG	2 cut(s)	1125, 1155
AcyI	GRCGYC	1 cut(s)	1604
AfaI	GTAC	7 cut(s)	364, 542, 1102, 1111, 1144, 1655, 2010
AfiI	CCNNNNNNNGG	4 cut(s)	353, 373, 797, 1026
AflII	CTTAAG	2 cut(s)	1541, 1562
AgsI	TTSAA	6 cut(s)	569, 857, 1241, 1429, 1835, 2030
AjiI	CACGTC	1 cut(s)	1821
AjnI	CCWGG	4 cut(s)	67, 118, 250, 1263
AjuI	GAANNNNNNNTTGG	2 cut(s)	1037, 1069
AloI	GAACNNNNNNTCC	2 cut(s)	424, 456
Alw21I	GWGCWC	1 cut(s)	209
Alw26I	GTCTC	3 cut(s)	351, 637, 1145
Alw44I	GTGCAC	1 cut(s)	205
AlwI	GGATC	4 cut(s)	130, 875, 1217, 1283
Aor13HI	TCCGGA	1 cut(s)	1617
ApaLI	GTGCAC	1 cut(s)	205
ApeKI	GCWGC	4 cut(s)	62, 514, 939, 1166
ApoI	RAATTY	6 cut(s)	454, 850, 923, 1476, 1519, 1597
AseI	ATTAAT	1 cut(s)	1383
Asp700I	GAANNNNTTC	2 cut(s)	80, 1419
Asp718I	GGTACC	1 cut(s)	362
AspA2I	CCTAGG	1 cut(s)	502
AspLEI	GCGC	4 cut(s)	62, 1606, 1942, 1944
AspS9I	GGNCC	3 cut(s)	1348, 1826, 1878
AsuC2I	CCSGG	1 cut(s)	260
AsuHPI	GGTGA	1 cut(s)	1617
AsuII	TTCGAA	2 cut(s)	74, 1572
AvaII	GGWCC	3 cut(s)	1348, 1826, 1878
AvrII	CCTAGG	1 cut(s)	502
BaeGI	GKGCMC	2 cut(s)	209, 1061
BanI	GGYRCC	2 cut(s)	362, 1603
BarI	GAAGNNNNNNTAC	2 cut(s)	766, 798
BbsI	GAAGAC	1 cut(s)	985
Bbv12I	GWGCWC	1 cut(s)	209
BbvI	GCAGC	4 cut(s)	49, 501, 926, 1178
BccI	CCATC	7 cut(s)	128, 195, 321, 657, 1133, 1196, 1802
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	4 cut(s)	69, 120, 252, 1265
BclI	TGATCA	2 cut(s)	961, 1896
BcnI	CCSGG	1 cut(s)	260
BcoDI	GTCTC	3 cut(s)	351, 637, 1145
BfmI	CTRYAG	1 cut(s)	891
BfoI	RGCGCY	1 cut(s)	1607
BfrI	CTTAAG	2 cut(s)	1541, 1562
BfuAI	ACCTGC	2 cut(s)	557, 1069
BisI	GCNGC	5 cut(s)	63, 515, 940, 1076, 1167
BlnI	CCTAGG	1 cut(s)	502
BlpI	GCTNAGC	1 cut(s)	240
BlsI	GCNGC	5 cut(s)	64, 516, 941, 1077, 1168
Bme1390I	CCNGG	5 cut(s)	69, 120, 252, 260, 1265
Bme18I	GGWCC	3 cut(s)	1348, 1826, 1878
BmgBI	CACGTC	1 cut(s)	1821
BmgT120I	GGNCC	3 cut(s)	1348, 1826, 1878
BmiI	GGNNCC	8 cut(s)	300, 364, 378, 1269, 1350, 1605, 1827, 1962
BmrFI	CCNGG	5 cut(s)	69, 120, 252, 260, 1265
BmrI	ACTGGG	1 cut(s)	438
BmsI	GCATC	7 cut(s)	850, 1217, 1447, 1688, 1772, 1772, 1853
BmuI	ACTGGG	1 cut(s)	438
BpiI	GAAGAC	1 cut(s)	985
BpmI	CTGGAG	2 cut(s)	409, 1323
Bpu1102I	GCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	2 cut(s)	74, 1572
BpuEI	CTTGAG	4 cut(s)	34, 113, 1532, 1727
BpuMI	CCSGG	1 cut(s)	260
BsaBI	GATNNNNATC	1 cut(s)	1461
BsaHI	GRCGYC	1 cut(s)	1604
BsaJI	CCNNGG	2 cut(s)	502, 1263
BsaWI	WCCGGW	1 cut(s)	1617
BsaXI	ACNNNNNCTCC	4 cut(s)	592, 622, 652, 682
Bsc4I	CCNNNNNNNGG	4 cut(s)	353, 373, 797, 1026
Bse1I	ACTGG	3 cut(s)	433, 563, 1881
Bse3DI	GCAATG	3 cut(s)	850, 863, 984
Bse8I	GATNNNNATC	1 cut(s)	1461
BseAI	TCCGGA	1 cut(s)	1617
BseBI	CCWGG	4 cut(s)	69, 120, 252, 1265
BseDI	CCNNGG	2 cut(s)	502, 1263
BseGI	GGATG	6 cut(s)	183, 443, 1679, 1787, 1868, 2008
BseJI	GATNNNNATC	1 cut(s)	1461
BseLI	CCNNNNNNNGG	4 cut(s)	353, 373, 797, 1026
BseMI	GCAATG	3 cut(s)	850, 863, 984
BseMII	CTCAG	2 cut(s)	254, 579
BseNI	ACTGG	3 cut(s)	433, 563, 1881
BsePI	GCGCGC	1 cut(s)	1940
BseRI	GAGGAG	3 cut(s)	923, 987, 1356
BseSI	GKGCMC	2 cut(s)	209, 1061
BseXI	GCAGC	4 cut(s)	49, 501, 926, 1178
BseYI	CCCAGC	1 cut(s)	755
BsgI	GTGCAG	1 cut(s)	48
Bsh1236I	CGCG	1 cut(s)	1942
BshNI	GGYRCC	2 cut(s)	362, 1603
BsiHKAI	GWGCWC	1 cut(s)	209
BsiSI	CCGG	2 cut(s)	259, 1618
BslFI	GGGAC	4 cut(s)	32, 329, 938, 1132
BslI	CCNNNNNNNGG	4 cut(s)	353, 373, 797, 1026
BsmAI	GTCTC	3 cut(s)	351, 637, 1145
BsmBI	CGTCTC	1 cut(s)	351
BsmFI	GGGAC	4 cut(s)	32, 329, 938, 1132
Bsp119I	TTCGAA	2 cut(s)	74, 1572
Bsp1286I	GDGCHC	2 cut(s)	209, 1061
Bsp13I	TCCGGA	1 cut(s)	1617
Bsp143I	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
Bsp1720I	GCTNAGC	1 cut(s)	240
BspACI	CCGC	4 cut(s)	528, 598, 956, 1076
BspCNI	CTCAG	2 cut(s)	253, 580
BspEI	TCCGGA	1 cut(s)	1617
BspFNI	CGCG	1 cut(s)	1942
BspHI	TCATGA	1 cut(s)	232
BspLI	GGNNCC	8 cut(s)	300, 364, 378, 1269, 1350, 1605, 1827, 1962
BspMI	ACCTGC	2 cut(s)	557, 1069
BspPI	GGATC	4 cut(s)	130, 875, 1217, 1283
BspT104I	TTCGAA	2 cut(s)	74, 1572
BspT107I	GGYRCC	2 cut(s)	362, 1603
BspTI	CTTAAG	2 cut(s)	1541, 1562
BsrDI	GCAATG	3 cut(s)	850, 863, 984
BsrI	ACTGG	3 cut(s)	433, 563, 1881
BssECI	CCNNGG	2 cut(s)	502, 1263
BssHII	GCGCGC	1 cut(s)	1940
BssMI	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
BssNI	GRCGYC	1 cut(s)	1604
BssT1I	CCWWGG	1 cut(s)	502
Bst2UI	CCWGG	4 cut(s)	69, 120, 252, 1265
Bst4CI	ACNGT	4 cut(s)	362, 800, 892, 1123
BstACI	GRCGYC	1 cut(s)	1604
BstAFI	CTTAAG	2 cut(s)	1541, 1562
BstBI	TTCGAA	2 cut(s)	74, 1572
BstC8I	GCNNGC	3 cut(s)	421, 1017, 1942
BstDEI	CTNAG	2 cut(s)	240, 588
BstEII	GGTNACC	1 cut(s)	705
BstENI	CCTNNNNNAGG	1 cut(s)	371
BstF5I	GGATG	6 cut(s)	183, 443, 1679, 1787, 1868, 2008
BstFNI	CGCG	1 cut(s)	1942
BstH2I	RGCGCY	1 cut(s)	1607
BstHHI	GCGC	4 cut(s)	62, 1606, 1942, 1944
BstKTI	GATC	9 cut(s)	110, 125, 157, 328, 883, 964, 1225, 1291, 1899
BstMAI	GTCTC	3 cut(s)	351, 637, 1145
BstMBI	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
BstMWI	GCNNNNNNNGC	5 cut(s)	584, 893, 1016, 1930, 1939
BstNI	CCWGG	4 cut(s)	69, 120, 252, 1265
BstNSI	RCATGY	2 cut(s)	423, 763
BstPI	GGTNACC	1 cut(s)	705
BstSCI	CCNGG	5 cut(s)	67, 118, 250, 258, 1263
BstSFI	CTRYAG	1 cut(s)	891
BstSLI	GKGCMC	2 cut(s)	209, 1061
BstUI	CGCG	1 cut(s)	1942
BstV1I	GCAGC	4 cut(s)	49, 501, 926, 1178
BstV2I	GAAGAC	1 cut(s)	985
BstX2I	RGATCY	2 cut(s)	122, 1288
BstYI	RGATCY	2 cut(s)	122, 1288
BtrI	CACGTC	1 cut(s)	1821
BtsCI	GGATG	6 cut(s)	183, 443, 1679, 1787, 1868, 2008
BtsI	GCAGTG	2 cut(s)	358, 1328
BtsIMutI	CAGTG	2 cut(s)	358, 1328
BveI	ACCTGC	2 cut(s)	557, 1069
Cac8I	GCNNGC	3 cut(s)	421, 1017, 1942
CciI	TCATGA	1 cut(s)	232
CfoI	GCGC	4 cut(s)	62, 1606, 1942, 1944
Cfr13I	GGNCC	3 cut(s)	1348, 1826, 1878
CsiI	ACCWGGT	1 cut(s)	67
Csp6I	GTAC	7 cut(s)	363, 541, 1101, 1110, 1143, 1654, 2009
CspCI	CAANNNNNGTGG	2 cut(s)	1316, 1351
CviAII	CATG	9 cut(s)	233, 420, 689, 760, 830, 943, 959, 1466, 1945
CviQI	GTAC	7 cut(s)	363, 541, 1101, 1110, 1143, 1654, 2009
DdeI	CTNAG	2 cut(s)	240, 588
DinI	GGCGCC	1 cut(s)	1605
DpnI	GATC	9 cut(s)	109, 124, 156, 327, 882, 963, 1224, 1290, 1898
DpnII	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
DraI	TTTAAA	2 cut(s)	1366, 1696
DrdI	GACNNNNNNGTC	1 cut(s)	1439
DseDI	GACNNNNNNGTC	1 cut(s)	1439
EciI	GGCGGA	1 cut(s)	543
Eco130I	CCWWGG	1 cut(s)	502
Eco32I	GATATC	2 cut(s)	385, 1623
Eco47I	GGWCC	3 cut(s)	1348, 1826, 1878
Eco57I	CTGAAG	2 cut(s)	1125, 1155
Eco91I	GGTNACC	1 cut(s)	705
EcoNI	CCTNNNNNAGG	1 cut(s)	371
EcoO109I	RGGNCCY	1 cut(s)	1826
EcoO65I	GGTNACC	1 cut(s)	705
EcoRII	CCWGG	4 cut(s)	67, 118, 250, 1263
EcoRV	GATATC	2 cut(s)	385, 1623
EcoT14I	CCWWGG	1 cut(s)	502
EcoT22I	ATGCAT	2 cut(s)	6, 865
EgeI	GGCGCC	1 cut(s)	1605
EheI	GGCGCC	1 cut(s)	1605
ErhI	CCWWGG	1 cut(s)	502
Esp3I	CGTCTC	1 cut(s)	351
FaeI	CATG	9 cut(s)	236, 423, 692, 763, 833, 946, 962, 1469, 1948
FalI	AAGNNNNNCTT	4 cut(s)	1494, 1526, 1917, 1949
FaqI	GGGAC	4 cut(s)	32, 329, 938, 1132
FatI	CATG	9 cut(s)	232, 419, 688, 759, 829, 942, 958, 1465, 1944
FauI	CCCGC	1 cut(s)	963
FauNDI	CATATG	1 cut(s)	484
FbaI	TGATCA	2 cut(s)	961, 1896
FblI	GTMKAC	2 cut(s)	1689, 1839
Fnu4HI	GCNGC	5 cut(s)	63, 515, 940, 1076, 1167
FokI	GGATG	6 cut(s)	170, 430, 1666, 1794, 1875, 2015
Fsp4HI	GCNGC	5 cut(s)	63, 515, 940, 1076, 1167
GlaI	GCGC	4 cut(s)	61, 1605, 1941, 1943
GluI	GCNGC	5 cut(s)	63, 515, 940, 1076, 1167
GsaI	CCCAGC	1 cut(s)	759
GsuI	CTGGAG	2 cut(s)	409, 1323
HaeII	RGCGCY	1 cut(s)	1607
HapII	CCGG	2 cut(s)	259, 1618
HhaI	GCGC	4 cut(s)	62, 1606, 1942, 1944
Hin1I	GRCGYC	1 cut(s)	1604
Hin1II	CATG	9 cut(s)	236, 423, 692, 763, 833, 946, 962, 1469, 1948
Hin6I	GCGC	4 cut(s)	60, 1604, 1940, 1942
HinP1I	GCGC	4 cut(s)	60, 1604, 1940, 1942
HincII	GTYRAC	5 cut(s)	873, 1690, 1840, 1848, 1996
HindII	GTYRAC	5 cut(s)	873, 1690, 1840, 1848, 1996
HindIII	AAGCTT	1 cut(s)	778
HinfI	GANTC	5 cut(s)	559, 1415, 1669, 1742, 1844
HpaII	CCGG	2 cut(s)	259, 1618
HphI	GGTGA	1 cut(s)	1617
Hpy166II	GTNNAC	8 cut(s)	207, 541, 873, 1348, 1690, 1840, 1848, 1996
Hpy188I	TCNGA	9 cut(s)	280, 660, 738, 922, 1044, 1174, 1255, 1758, 1969
Hpy188III	TCNNGA	9 cut(s)	158, 233, 380, 388, 771, 1511, 1618, 1706, 1779
Hpy8I	GTNNAC	8 cut(s)	207, 541, 873, 1348, 1690, 1840, 1848, 1996
Hpy99I	CGWCG	2 cut(s)	1825, 1844
HpyCH4III	ACNGT	4 cut(s)	362, 800, 892, 1123
HpyCH4IV	ACGT	3 cut(s)	1820, 2016, 2038
HpyF10VI	GCNNNNNNNGC	5 cut(s)	584, 893, 1016, 1930, 1939
HpyF3I	CTNAG	2 cut(s)	240, 588
HpySE526I	ACGT	3 cut(s)	1820, 2016, 2038
Hsp92I	GRCGYC	1 cut(s)	1604
Hsp92II	CATG	9 cut(s)	236, 423, 692, 763, 833, 946, 962, 1469, 1948
HspAI	GCGC	4 cut(s)	60, 1604, 1940, 1942
KasI	GGCGCC	1 cut(s)	1603
Kpn2I	TCCGGA	1 cut(s)	1617
KpnI	GGTACC	1 cut(s)	366
Ksp22I	TGATCA	2 cut(s)	961, 1896
Kzo9I	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
LmnI	GCTCC	7 cut(s)	287, 298, 382, 974, 1216, 1267, 1960
Lsp1109I	GCAGC	4 cut(s)	49, 501, 926, 1178
LweI	GCATC	7 cut(s)	850, 1217, 1447, 1688, 1772, 1772, 1853
MabI	ACCWGGT	1 cut(s)	67
MaeII	ACGT	3 cut(s)	1820, 2016, 2038
MaeIII	GTNAC	4 cut(s)	223, 705, 1231, 1441
MalI	GATC	9 cut(s)	109, 124, 156, 327, 882, 963, 1224, 1290, 1898
MboI	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
MfeI	CAATTG	2 cut(s)	86, 1905
MflI	RGATCY	2 cut(s)	122, 1288
MhlI	GDGCHC	2 cut(s)	209, 1061
Mly113I	GGCGCC	1 cut(s)	1604
MlyI	GAGTC	1 cut(s)	1853
MmeI	TCCRAC	1 cut(s)	1510
Mph1103I	ATGCAT	2 cut(s)	6, 865
MroI	TCCGGA	1 cut(s)	1617
MroXI	GAANNNNTTC	2 cut(s)	80, 1419
MseI	TTAA	9 cut(s)	408, 519, 672, 1184, 1365, 1383, 1542, 1563, 1695
MslI	CAYNNNNRTG	2 cut(s)	720, 834
MspCI	CTTAAG	2 cut(s)	1541, 1562
MspI	CCGG	2 cut(s)	259, 1618
MspR9I	CCNGG	5 cut(s)	69, 120, 252, 260, 1265
MunI	CAATTG	2 cut(s)	86, 1905
MvaI	CCWGG	4 cut(s)	69, 120, 252, 1265
MvnI	CGCG	1 cut(s)	1942
MwoI	GCNNNNNNNGC	5 cut(s)	584, 893, 1016, 1930, 1939
NarI	GGCGCC	1 cut(s)	1604
NciI	CCSGG	1 cut(s)	260
NdeI	CATATG	1 cut(s)	484
NdeII	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
NlaIII	CATG	9 cut(s)	236, 423, 692, 763, 833, 946, 962, 1469, 1948
NlaIV	GGNNCC	8 cut(s)	300, 364, 378, 1269, 1350, 1605, 1827, 1962
NmuCI	GTSAC	3 cut(s)	223, 1231, 1441
NsiI	ATGCAT	2 cut(s)	6, 865
NspI	RCATGY	2 cut(s)	423, 763
NspV	TTCGAA	2 cut(s)	74, 1572
PaeI	GCATGC	1 cut(s)	423
PagI	TCATGA	1 cut(s)	232
PauI	GCGCGC	1 cut(s)	1940
PdmI	GAANNNNTTC	2 cut(s)	80, 1419
PfeI	GAWTC	4 cut(s)	559, 1415, 1669, 1742
PkrI	GCNGC	5 cut(s)	64, 516, 941, 1077, 1168
PleI	GAGTC	1 cut(s)	1852
PluTI	GGCGCC	1 cut(s)	1607
PpsI	GAGTC	1 cut(s)	1852
PpuMI	RGGWCCY	1 cut(s)	1826
PshBI	ATTAAT	1 cut(s)	1383
Psp1406I	AACGTT	1 cut(s)	2038
Psp5II	RGGWCCY	1 cut(s)	1826
Psp6I	CCWGG	4 cut(s)	67, 118, 250, 1263
PspEI	GGTNACC	1 cut(s)	705
PspFI	CCCAGC	1 cut(s)	755
PspGI	CCWGG	4 cut(s)	67, 118, 250, 1263
PspN4I	GGNNCC	8 cut(s)	300, 364, 378, 1269, 1350, 1605, 1827, 1962
PspPI	GGNCC	3 cut(s)	1348, 1826, 1878
PspPPI	RGGWCCY	1 cut(s)	1826
PsuI	RGATCY	2 cut(s)	122, 1288
PteI	GCGCGC	1 cut(s)	1940
RsaI	GTAC	7 cut(s)	364, 542, 1102, 1111, 1144, 1655, 2010
RsaNI	GTAC	7 cut(s)	363, 541, 1101, 1110, 1143, 1654, 2009
RseI	CAYNNNNRTG	2 cut(s)	720, 834
SalI	GTCGAC	2 cut(s)	1688, 1838
SaqAI	TTAA	9 cut(s)	408, 519, 672, 1184, 1365, 1383, 1542, 1563, 1695
SatI	GCNGC	5 cut(s)	63, 515, 940, 1076, 1167
Sau3AI	GATC	9 cut(s)	107, 122, 154, 325, 880, 961, 1222, 1288, 1896
Sau96I	GGNCC	3 cut(s)	1348, 1826, 1878
SchI	GAGTC	1 cut(s)	1853
ScrFI	CCNGG	5 cut(s)	69, 120, 252, 260, 1265
SduI	GDGCHC	2 cut(s)	209, 1061
SexAI	ACCWGGT	1 cut(s)	67
SfaNI	GCATC	7 cut(s)	850, 1217, 1447, 1688, 1772, 1772, 1853
SfcI	CTRYAG	1 cut(s)	891
SfoI	GGCGCC	1 cut(s)	1605
SfuI	TTCGAA	2 cut(s)	74, 1572
SinI	GGWCC	3 cut(s)	1348, 1826, 1878
SmiMI	CAYNNNNRTG	2 cut(s)	720, 834
SmlI	CTYRAG	6 cut(s)	13, 92, 1511, 1541, 1562, 1706
SmoI	CTYRAG	6 cut(s)	13, 92, 1511, 1541, 1562, 1706
SphI	GCATGC	1 cut(s)	423
SsiI	CCGC	4 cut(s)	528, 598, 956, 1076
SspDI	GGCGCC	1 cut(s)	1603
StyD4I	CCNGG	5 cut(s)	67, 118, 250, 258, 1263
StyI	CCWWGG	1 cut(s)	502
TaaI	ACNGT	4 cut(s)	362, 800, 892, 1123
TaiI	ACGT	3 cut(s)	1823, 2019, 2041
TaqI	TCGA	4 cut(s)	74, 1572, 1689, 1839
TatI	WGTACW	1 cut(s)	540
TauI	GCSGC	1 cut(s)	1078
TfiI	GAWTC	4 cut(s)	559, 1415, 1669, 1742
Tru1I	TTAA	9 cut(s)	408, 519, 672, 1184, 1365, 1383, 1542, 1563, 1695
Tru9I	TTAA	9 cut(s)	408, 519, 672, 1184, 1365, 1383, 1542, 1563, 1695
TscAI	CASTG	2 cut(s)	358, 1328
TseFI	GTSAC	3 cut(s)	223, 1231, 1441
TseI	GCWGC	4 cut(s)	62, 514, 939, 1166
Tsp45I	GTSAC	3 cut(s)	223, 1231, 1441
TspDTI	ATGAA	8 cut(s)	128, 249, 432, 572, 588, 973, 1386, 1428
TspGWI	ACGGA	2 cut(s)	1516, 1641
TspRI	CASTG	2 cut(s)	358, 1328
Vha464I	CTTAAG	2 cut(s)	1541, 1562
VneI	GTGCAC	1 cut(s)	205
VpaK11BI	GGWCC	3 cut(s)	1348, 1826, 1878
VspI	ATTAAT	1 cut(s)	1383
XagI	CCTNNNNNAGG	1 cut(s)	371
XapI	RAATTY	6 cut(s)	454, 850, 923, 1476, 1519, 1597
XceI	RCATGY	2 cut(s)	423, 763
XmaJI	CCTAGG	1 cut(s)	502
XmiI	GTMKAC	2 cut(s)	1689, 1839
XmnI	GAANNNNTTC	2 cut(s)	80, 1419
Zsp2I	ATGCAT	2 cut(s)	6, 865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.