AT2G22780

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014980
Physical Location & Seq
Reverse (-)
9689295 .. 9692154
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22780.1

Sequence Viewer

Length: 1065 bp
ATGGATCCAAACCAACGTATCGCGAGAATCTCTGCTCATCTCAATCCTCCTAATCTTCATAATCAGATTGCTGACGGGTCAGGTTTGAATCGGGTGGCTTGTCGGGCAAAAGGTGGATCACCCGGATTCAAAGTGGCGATACTTGGAGCAGCTGGTGGAATTGGACAACCTCTTGCGATGTTGATGAAGATGAATCCTTTGGTTTCGGTTCTTCATCTCTATGATGTTGCTAATGCTCCTGGTGTTACTGCTGATATTAGTCATATGGATACTAGTGCCGTTGTTCGTGGATTTCTCGGGCAGCCGCAGTTAGAGGAAGCACTTACGGGTATGGATTTAGTGATCATACCTGCTGGTGTTCCGAGGAAACCAGGGATGACGAGGGATGATCTGTTTAACATTAATGCTGGGATTGTGAGGACACTCTCTGAAGCTATAGCTAAATGTTGTCCTAAAGCAATTGTGAATATAATCAGTAATCCGGTGAACTCCACGGTGCCAATCGCAGCTGAGGTTTTCAAGAAAGCTGGAACCTTTGATCCAAAGAAACTCATGGGTGTCACTATGCTTGATGTTGTTAGAGCTAATACCTTTGTGGCGGAAGTAATGAGTCTTGATCCCCGTGAAGTTGAAGTTCCGGTTGTTGGAGGACACGCAGGAGTTACGATTTTACCACTGCTTTCGCAGGTGAAACCTCCTTGCTCGTTCACTCAAAAAGAGATTGAATATCTCACAGACCGCATCCAAAACGGTGGCACTGAAGTTGTTGAGGCTAAAGCTGGAGCAGGTTCTGCAACACTATCCATGGCATATGCAGCAGTGGAGTTTGCAGATGCTTGCCTCAGGGGTCTACGAGGTGATGCAAACATCGTTGAGTGCGCATATGTGGCATCCCATGTGACTGAGCTTCCCTTCTTCGCATCGAAGGTGCGTCTGGGACGATGTGGGATCGATGAAGTGTACGGCCTTGGACCATTGAACGAATATGAGAGGATGGGATTAGAGAAGGCAAAGAAAGAGCTTTCAGTAAGTATTCATAAAGGTGTTACCTTTGCGAAGAAATAA

Protein Analysis

354

Amino Acids

37.47

Weight (kDa)

8.11

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 44 - 186 1.4e-47 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 188 - 351 1.5e-42 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 676
Acc16I TGCGCA 1 cut(s) 880
Acc36I ACCTGC 3 cut(s) 358, 676, 776
AccB1I GGYRCC 1 cut(s) 496
AccI GTMKAC 1 cut(s) 850
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 305, 599, 739
AclWI GGATC 5 cut(s) 12, 124, 533, 611, 956
AcuI CTGAAG 2 cut(s) 450, 780
AfaI GTAC 1 cut(s) 962
AfiI CCNNNNNNNGG 1 cut(s) 644
AgsI TTSAA 6 cut(s) 88, 130, 520, 632, 725, 979
AhlI ACTAGT 1 cut(s) 272
AjnI CCWGG 2 cut(s) 238, 370
AloI GAACNNNNNNTCC 2 cut(s) 523, 555
AluBI AGCT 9 cut(s) 152, 434, 440, 509, 527, 584, 779, 907, 1021
AluI AGCT 9 cut(s) 152, 434, 440, 509, 527, 584, 779, 907, 1021
AlwI GGATC 5 cut(s) 12, 124, 533, 611, 956
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 1 cut(s) 964
ApeKI GCWGC 4 cut(s) 149, 301, 506, 815
AseI ATTAAT 1 cut(s) 402
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 1 cut(s) 971
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 4 cut(s) 111, 496, 700, 869
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 971
AxyI CCTNAGG 1 cut(s) 842
BaeI ACNNNNGTAYC 1 cut(s) 34
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 496
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 161, 313, 518, 827
BccI CCATC 1 cut(s) 988
BceAI ACGGC 2 cut(s) 263, 979
BciT130I CCWGG 2 cut(s) 240, 372
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 123
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 435
BfuAI ACCTGC 3 cut(s) 358, 676, 776
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 5 cut(s) 150, 302, 305, 507, 816
BlsI GCNGC 5 cut(s) 151, 303, 306, 508, 817
Bme1390I CCNGG 3 cut(s) 123, 240, 372
Bme18I GGWCC 1 cut(s) 971
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 971
BmiI GGNNCC 3 cut(s) 6, 498, 532
BmrFI CCNGG 3 cut(s) 123, 240, 372
BmsI GCATC 5 cut(s) 750, 823, 850, 899, 929
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 123
Bsa29I ATCGAT 1 cut(s) 951
BsaJI CCNNGG 5 cut(s) 362, 371, 492, 804, 967
BsaWI WCCGGW 2 cut(s) 481, 637
Bsc4I CCNNNNNNNGG 1 cut(s) 644
Bse21I CCTNAGG 1 cut(s) 842
BseBI CCWGG 2 cut(s) 240, 372
BseCI ATCGAT 1 cut(s) 951
BseDI CCNNGG 5 cut(s) 362, 371, 492, 804, 967
BseGI GGATG 5 cut(s) 381, 391, 741, 890, 999
BseLI CCNNNNNNNGG 1 cut(s) 644
BseMII CTCAG 3 cut(s) 501, 856, 894
BseXI GCAGC 4 cut(s) 161, 313, 518, 827
BseYI CCCAGC 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 966
BshNI GGYRCC 1 cut(s) 496
BshVI ATCGAT 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 3 cut(s) 123, 482, 638
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 1 cut(s) 644
BsmFI GGGAC 1 cut(s) 951
BsnI GGCC 1 cut(s) 966
BsoBI CYCGRG 1 cut(s) 296
Bsp143I GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
Bsp19I CCATGG 1 cut(s) 804
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 3 cut(s) 305, 599, 739
BspANI GGCC 1 cut(s) 966
BspCNI CTCAG 3 cut(s) 502, 855, 895
BspDI ATCGAT 1 cut(s) 951
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 6, 498, 532
BspMI ACCTGC 3 cut(s) 358, 676, 776
BspPI GGATC 5 cut(s) 12, 124, 533, 611, 956
BspT107I GGYRCC 1 cut(s) 496
BssECI CCNNGG 5 cut(s) 362, 371, 492, 804, 967
BssMI GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
BssT1I CCWWGG 2 cut(s) 804, 967
Bst2UI CCWGG 2 cut(s) 240, 372
Bst4CI ACNGT 2 cut(s) 496, 752
BstAPI GCANNNNNTGC 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 838
BstDEI CTNAG 3 cut(s) 510, 842, 903
BstDSI CCRYGG 2 cut(s) 492, 804
BstF5I GGATG 5 cut(s) 381, 391, 741, 890, 999
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 7 cut(s) 7, 119, 345, 391, 541, 619, 951
BstMBI GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
BstMWI GCNNNNNNNGC 4 cut(s) 104, 791, 815, 887
BstNI CCWGG 2 cut(s) 240, 372
BstSCI CCNGG 3 cut(s) 121, 238, 370
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 4 cut(s) 161, 313, 518, 827
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 752
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 951
Bsu36I CCTNAGG 1 cut(s) 842
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 966
BsuTUI ATCGAT 1 cut(s) 951
BtgI CCRYGG 2 cut(s) 492, 804
BtgZI GCGATG 1 cut(s) 191
BtsCI GGATG 5 cut(s) 381, 391, 741, 890, 999
BtsI GCAGTG 2 cut(s) 674, 825
BtsIMutI CAGTG 3 cut(s) 674, 756, 825
BtuMI TCGCGA 1 cut(s) 23
BveI ACCTGC 3 cut(s) 358, 676, 776
Cac8I GCNNGC 1 cut(s) 838
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 1 cut(s) 881
Cfr13I GGNCC 1 cut(s) 971
ClaI ATCGAT 1 cut(s) 951
CseI GACGC 1 cut(s) 920
Csp6I GTAC 1 cut(s) 961
CviAII CATG 3 cut(s) 553, 805, 896
CviQI GTAC 1 cut(s) 961
DdeI CTNAG 3 cut(s) 510, 842, 903
DpnI GATC 7 cut(s) 6, 118, 344, 390, 540, 618, 950
DpnII GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 2 cut(s) 804, 967
Eco47I GGWCC 1 cut(s) 971
Eco57I CTGAAG 2 cut(s) 450, 780
Eco81I CCTNAGG 1 cut(s) 842
Eco88I CYCGRG 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 238, 370
EcoT14I CCWWGG 2 cut(s) 804, 967
ErhI CCWWGG 2 cut(s) 804, 967
FaeI CATG 3 cut(s) 556, 808, 899
FaqI GGGAC 1 cut(s) 951
FatI CATG 3 cut(s) 552, 804, 895
FauNDI CATATG 3 cut(s) 264, 811, 883
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 1 cut(s) 850
Fnu4HI GCNGC 5 cut(s) 150, 302, 305, 507, 816
FokI GGATG 5 cut(s) 388, 398, 728, 877, 1006
Fsp4HI GCNGC 5 cut(s) 150, 302, 305, 507, 816
FspAI RTGCGCAY 1 cut(s) 880
FspBI CTAG 1 cut(s) 273
FspI TGCGCA 1 cut(s) 880
GlaI GCGC 1 cut(s) 880
GluI GCNGC 5 cut(s) 150, 302, 305, 507, 816
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 1 cut(s) 966
HapII CCGG 3 cut(s) 123, 482, 638
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 881
Hin1II CATG 3 cut(s) 556, 808, 899
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HinfI GANTC 5 cut(s) 27, 88, 126, 193, 610
HpaII CCGG 3 cut(s) 123, 482, 638
HphI GGTGA 4 cut(s) 111, 496, 700, 869
Hpy166II GTNNAC 4 cut(s) 487, 708, 851, 961
Hpy188I TCNGA 3 cut(s) 66, 363, 430
Hpy188III TCNNGA 3 cut(s) 22, 520, 614
Hpy8I GTNNAC 4 cut(s) 487, 708, 851, 961
HpyAV CCTTC 3 cut(s) 919, 922, 1000
HpyCH4III ACNGT 2 cut(s) 496, 752
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 4 cut(s) 794, 815, 830, 863
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 791, 815, 887
HpyF3I CTNAG 3 cut(s) 510, 842, 903
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 3 cut(s) 556, 808, 899
HspAI GCGC 1 cut(s) 879
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
LmnI GCTCC 3 cut(s) 146, 241, 782
Lsp1109I GCAGC 4 cut(s) 161, 313, 518, 827
LweI GCATC 5 cut(s) 750, 823, 850, 899, 929
MaeI CTAG 1 cut(s) 273
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 5 cut(s) 244, 559, 661, 898, 1045
MalI GATC 7 cut(s) 6, 118, 344, 390, 540, 618, 950
MboI GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
MboII GAAGA 4 cut(s) 47, 199, 203, 907
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 4
MluCI AATT 2 cut(s) 159, 459
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 625
MseI TTAA 2 cut(s) 396, 402
MslI CAYNNNNRTG 2 cut(s) 219, 1041
MspA1I CMGCKG 2 cut(s) 152, 509
MspI CCGG 3 cut(s) 123, 482, 638
MspR9I CCNGG 3 cut(s) 123, 240, 372
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 2 cut(s) 240, 372
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 104, 791, 815, 887
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 804
NdeI CATATG 3 cut(s) 264, 811, 883
NdeII GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
NlaIII CATG 3 cut(s) 556, 808, 899
NlaIV GGNNCC 3 cut(s) 6, 498, 532
NmuCI GTSAC 2 cut(s) 559, 898
NruI TCGCGA 1 cut(s) 23
NsbI TGCGCA 1 cut(s) 880
PaqCI CACCTGC 1 cut(s) 676
PfeI GAWTC 4 cut(s) 27, 88, 126, 193
PkrI GCNGC 5 cut(s) 151, 303, 306, 508, 817
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
PshBI ATTAAT 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 238, 370
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 2 cut(s) 238, 370
PspN4I GGNNCC 3 cut(s) 6, 498, 532
PspPI GGNCC 1 cut(s) 971
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 2 cut(s) 152, 509
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 1 cut(s) 962
RsaNI GTAC 1 cut(s) 961
RseI CAYNNNNRTG 2 cut(s) 219, 1041
SaqAI TTAA 2 cut(s) 396, 402
SatI GCNGC 5 cut(s) 150, 302, 305, 507, 816
Sau3AI GATC 7 cut(s) 4, 116, 342, 388, 538, 616, 948
Sau96I GGNCC 1 cut(s) 971
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 123, 240, 372
SfaNI GCATC 5 cut(s) 750, 823, 850, 899, 929
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 971
SmiMI CAYNNNNRTG 2 cut(s) 219, 1041
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 2 cut(s) 159, 459
SsiI CCGC 3 cut(s) 305, 599, 739
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 3 cut(s) 121, 238, 370
StyI CCWWGG 2 cut(s) 804, 967
TaaI ACNGT 2 cut(s) 496, 752
TaiI ACGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 923, 951
TasI AATT 2 cut(s) 159, 459
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 4 cut(s) 27, 88, 126, 193
Tru1I TTAA 2 cut(s) 396, 402
Tru9I TTAA 2 cut(s) 396, 402
TscAI CASTG 3 cut(s) 681, 763, 825
TseFI GTSAC 2 cut(s) 559, 898
TseI GCWGC 4 cut(s) 149, 301, 506, 815
Tsp45I GTSAC 2 cut(s) 559, 898
TspDTI ATGAA 6 cut(s) 47, 200, 203, 206, 969, 1025
TspRI CASTG 3 cut(s) 681, 763, 825
VpaK11BI GGWCC 1 cut(s) 971
VspI ATTAAT 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 850
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.