pycom02g15840

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014980
Physical Location & Seq
Forward (+)
12841889 .. 12844775
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15840.9

Sequence Viewer

Length: 1362 bp
ATGGAAATGGGAGGGCAGATAATCCACCCCCTTTCCCATAACCCAATCGACAAGGATTGTCTCATCGAAAATGAGAGCAGACAATCCTTGTCCTCACCCAATTGCGGTGAAGCACCAGAAGTCAGAAAGTTCGGATATTGGGTTTTGGGAACTTCAACTTGGGATAAGATCAGTTTCCATATTCCACACCGCCCAATTTCCCAGCAAAGCTTTCCCGTCTTCACTCATCCTCTCCACCCCCTCTCTCTGCTCATTTATTTATCGACGATCGGATCAATTTTCGCCACAAAAAATCACACGATGCAGCAGCAGACGAGCTCAGAAGTGAACGCGAACCAACGGATTGCTAGAATTTCCGCCCATTTGGACCCACCAAATCTCCAGATGGAGAACGCTGCCACTGTTAGCCGCTTGAATTGCCGCGCCAAGGGCGGCGCTGCTGGTTTCAAAGTAGCCATACTTGGAGCAGCCGGCGGCATAGGCCAGCCTCTCGCCATGTTAATGAAGATGAACCCCTTGGTCTCCGTTCTCCACCTATACGACGTCGTCAACACCCCCGGCGTCACCTCCGATATCAGCCACATGGACACCGGCGCCGTAGTGCGCGGGTTTTTGGGGCAGCAGCAGCTGGAGGAAGCGCTGACCGGAATGGAGCTGGTGATCATTCCGGCCGGTGTGCCCCGAAAACCCGGAATGACAAGGGACGATTTGTTCAACATCAATGCCGGAATAGTCAAGACGATCAGTGAAGCAATCGCCAGGTGCTGTCCGTATGCCATTGTTAACGTGATTAGCAACCCTGTGAACTCCACAGTTCCCATTGCTGCTGAAGTTTTCAAAAAAGCTGGTACCTTTGATCGATCTTCTAAGAAACTATTGGGAGTCACCATGCTTGATGTTGTTAGAGCCAATACTTTTGTGGGAGAGATTCTGGGTCTTGATCCTAGGGACATCGACGTTCCCGTTGTTGGGGGTCATGCAGGGGTTACAATTTTGCCTCTTCTGTCTCAGGTTAAACCTCCATGCTCTTTCACCCCAAAAGAAGTTGATTACCTAACAGATCGCATTCAGAATGGTGGCACTGAGGTGGTTGAGGCAAAAGCTGGAGCTGGTTCTGCAACATTATCAATGGCATATGCTGCTGTTAAATTTGCAGATGCATGCCTTCGTGGCTTGCGAGGATATGCTGGGGTTGTGACTGAACTTCCCTTCTTTGCATCCAAGGTGCGGCTTGGCCGTACTGGAGCTGAGGAAATATATCCTCTTGGCCCCCTGAATGAGTATGAGAGGGCTGGCTTGGAGAAGGCAAAGAAAGAACTAGCAGCAAGCATTCAGAAGGGGGTTTCTTTCGTCAGAAAATGA

Protein Analysis

454

Amino Acids

48.6

Weight (kDa)

8.57

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 546
Acc65I GGTACC 1 cut(s) 848
AccB1I GGYRCC 2 cut(s) 593, 848
AccII CGCG 3 cut(s) 332, 423, 606
AciI CCGC 9 cut(s) 105, 190, 357, 409, 421, 432, 474, 606, 1228
AclWI GGATC 2 cut(s) 280, 935
AcoI YGGCCR 2 cut(s) 669, 1234
AcsI RAATTY 2 cut(s) 351, 1148
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 3 cut(s) 543, 561, 594
AfaI GTAC 2 cut(s) 850, 1240
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 5 cut(s) 104, 427, 968, 969, 1227
AgsI TTSAA 5 cut(s) 156, 415, 448, 715, 838
AjnI CCWGG 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 142, 174
AleI CACNNNNGTG 1 cut(s) 1085
AloI GAACNNNNNNTCC 2 cut(s) 695, 727
AluBI AGCT 8 cut(s) 210, 318, 628, 655, 845, 1103, 1109, 1247
AluI AGCT 8 cut(s) 210, 318, 628, 655, 845, 1103, 1109, 1247
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 3 cut(s) 65, 526, 1011
AlwI GGATC 2 cut(s) 280, 935
AlwNI CAGNNNCTG 2 cut(s) 628, 765
Aor51HI AGCGCT 1 cut(s) 639
AoxI GGCC 4 cut(s) 481, 669, 1234, 1267
ApoI RAATTY 2 cut(s) 351, 1148
Asp718I GGTACC 1 cut(s) 848
AspA2I CCTAGG 1 cut(s) 944
AspLEI GCGC 5 cut(s) 425, 437, 596, 606, 640
AspS9I GGNCC 2 cut(s) 367, 1268
AsuC2I CCSGG 2 cut(s) 558, 690
AsuHPI GGTGA 6 cut(s) 87, 119, 556, 670, 877, 1024
AvaII GGWCC 1 cut(s) 367
AvrII CCTAGG 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 681
BanI GGYRCC 2 cut(s) 593, 848
BanII GRGCYC 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 1248
BccI CCATC 1 cut(s) 379
BceAI ACGGC 2 cut(s) 581, 1221
BciT130I CCWGG 1 cut(s) 760
BclI TGATCA 1 cut(s) 660
BcnI CCSGG 2 cut(s) 558, 690
BcoDI GTCTC 3 cut(s) 65, 526, 1011
BfaI CTAG 3 cut(s) 348, 945, 1319
BfoI RGCGCY 3 cut(s) 438, 597, 641
BglI GCCNNNNNGGC 1 cut(s) 1170
BlnI CCTAGG 1 cut(s) 944
Bme1390I CCNGG 3 cut(s) 558, 690, 760
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 2 cut(s) 367, 1268
BmiI GGNNCC 4 cut(s) 369, 595, 850, 1270
BmrFI CCNGG 3 cut(s) 558, 690, 760
BmsI GCATC 3 cut(s) 291, 1147, 1226
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 4 cut(s) 365, 650, 1125, 1263
Bpu10I CCTNAGC 1 cut(s) 1248
BpuMI CCSGG 2 cut(s) 558, 690
Bsa29I ATCGAT 1 cut(s) 859
BsaHI GRCGYC 3 cut(s) 543, 561, 594
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 5 cut(s) 426, 516, 556, 944, 1221
BsaWI WCCGGW 1 cut(s) 644
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 427, 968, 969, 1227
Bse118I RCCGGY 3 cut(s) 470, 590, 671
Bse1I ACTGG 1 cut(s) 1246
Bse3DI GCAATG 1 cut(s) 819
BseBI CCWGG 1 cut(s) 760
BseCI ATCGAT 1 cut(s) 859
BseDI CCNNGG 5 cut(s) 426, 516, 556, 944, 1221
BseGI GGATG 2 cut(s) 226, 1217
BseLI CCNNNNNNNGG 5 cut(s) 104, 427, 968, 969, 1227
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 4 cut(s) 333, 1022, 1074, 1239
BseNI ACTGG 1 cut(s) 1246
BseSI GKGCMC 1 cut(s) 681
BseX3I CGGCCG 1 cut(s) 669
BseYI CCCAGC 2 cut(s) 201, 1187
Bsh1236I CGCG 3 cut(s) 332, 423, 606
Bsh1285I CGRYCG 2 cut(s) 270, 672
BshFI GGCC 4 cut(s) 483, 671, 1236, 1269
BshNI GGYRCC 2 cut(s) 593, 848
BshVI ATCGAT 1 cut(s) 859
BsiEI CGRYCG 2 cut(s) 270, 672
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 8 cut(s) 471, 558, 591, 645, 668, 672, 690, 726
BslFI GGGAC 2 cut(s) 716, 962
BslI CCNNNNNNNGG 5 cut(s) 104, 427, 968, 969, 1227
BsmAI GTCTC 3 cut(s) 65, 526, 1011
BsmFI GGGAC 2 cut(s) 716, 962
BsmI GAATGC 2 cut(s) 1065, 1329
BsnI GGCC 4 cut(s) 483, 671, 1236, 1269
Bso31I GGTCTC 1 cut(s) 526
Bsp1286I GDGCHC 2 cut(s) 320, 681
Bsp143I GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
BspACI CCGC 9 cut(s) 105, 190, 357, 409, 421, 432, 474, 606, 1228
BspANI GGCC 4 cut(s) 483, 671, 1236, 1269
BspCNI CTCAG 4 cut(s) 332, 1021, 1075, 1240
BspDI ATCGAT 1 cut(s) 859
BspFNI CGCG 3 cut(s) 332, 423, 606
BspLI GGNNCC 4 cut(s) 369, 595, 850, 1270
BspPI GGATC 2 cut(s) 280, 935
BspT107I GGYRCC 2 cut(s) 593, 848
BspTNI GGTCTC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 819
BsrFI RCCGGY 3 cut(s) 470, 590, 671
BsrI ACTGG 1 cut(s) 1246
BssAI RCCGGY 3 cut(s) 470, 590, 671
BssECI CCNNGG 5 cut(s) 426, 516, 556, 944, 1221
BssMI GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
BssNI GRCGYC 3 cut(s) 543, 561, 594
BssT1I CCWWGG 4 cut(s) 426, 516, 944, 1221
Bst2UI CCWGG 1 cut(s) 760
Bst4CI ACNGT 2 cut(s) 403, 814
Bst6I CTCTTC 1 cut(s) 1005
BstACI GRCGYC 3 cut(s) 543, 561, 594
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstC8I GCNNGC 6 cut(s) 472, 485, 1162, 1175, 1294, 1327
BstDEI CTNAG 5 cut(s) 319, 867, 1008, 1083, 1248
BstF5I GGATG 2 cut(s) 226, 1217
BstFNI CGCG 3 cut(s) 332, 423, 606
BstH2I RGCGCY 3 cut(s) 438, 597, 641
BstHHI GCGC 5 cut(s) 425, 437, 596, 606, 640
BstKTI GATC 9 cut(s) 171, 270, 275, 663, 744, 859, 863, 943, 1063
BstMAI GTCTC 3 cut(s) 65, 526, 1011
BstMBI GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
BstMCI CGRYCG 2 cut(s) 270, 672
BstMWI GCNNNNNNNGC 7 cut(s) 417, 429, 480, 625, 1115, 1139, 1170
BstNI CCWGG 1 cut(s) 760
BstNSI RCATGY 1 cut(s) 1164
BstSCI CCNGG 3 cut(s) 556, 688, 758
BstSLI GKGCMC 1 cut(s) 681
BstUI CGCG 3 cut(s) 332, 423, 606
BstV2I GAAGAC 1 cut(s) 211
BstZI CGGCCG 1 cut(s) 669
Bsu15I ATCGAT 1 cut(s) 859
BsuRI GGCC 4 cut(s) 483, 671, 1236, 1269
BsuTUI ATCGAT 1 cut(s) 859
BtsCI GGATG 2 cut(s) 226, 1217
BtsIMutI CAGTG 3 cut(s) 399, 751, 1080
Cac8I GCNNGC 6 cut(s) 472, 485, 1162, 1175, 1294, 1327
CaiI CAGNNNCTG 2 cut(s) 628, 765
CfoI GCGC 5 cut(s) 425, 437, 596, 606, 640
Cfr10I RCCGGY 3 cut(s) 470, 590, 671
Cfr13I GGNCC 2 cut(s) 367, 1268
ClaI ATCGAT 1 cut(s) 859
CseI GACGC 1 cut(s) 550
Csp6I GTAC 2 cut(s) 849, 1239
CviAII CATG 6 cut(s) 496, 583, 889, 977, 1023, 1161
CviQI GTAC 2 cut(s) 849, 1239
DdeI CTNAG 5 cut(s) 319, 867, 1008, 1083, 1248
DinI GGCGCC 1 cut(s) 595
DpnI GATC 9 cut(s) 170, 269, 274, 662, 743, 858, 862, 942, 1062
DpnII GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
EaeI YGGCCR 2 cut(s) 669, 1234
EagI CGGCCG 1 cut(s) 669
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 669
Eco130I CCWWGG 4 cut(s) 426, 516, 944, 1221
Eco24I GRGCYC 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 367
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 669
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 758
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 4 cut(s) 426, 516, 944, 1221
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 1 cut(s) 320
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
ErhI CCWWGG 4 cut(s) 426, 516, 944, 1221
FaeI CATG 6 cut(s) 499, 586, 892, 980, 1026, 1164
FaqI GGGAC 2 cut(s) 716, 962
FatI CATG 6 cut(s) 495, 582, 888, 976, 1022, 1160
FauI CCCGC 1 cut(s) 599
FauNDI CATATG 1 cut(s) 1135
FbaI TGATCA 1 cut(s) 660
FokI GGATG 2 cut(s) 213, 1204
FriOI GRGCYC 1 cut(s) 320
FspBI CTAG 3 cut(s) 348, 945, 1319
GlaI GCGC 5 cut(s) 424, 436, 595, 605, 639
GsaI CCCAGC 2 cut(s) 205, 1191
GsuI CTGGAG 4 cut(s) 365, 650, 1125, 1263
HaeII RGCGCY 3 cut(s) 438, 597, 641
HaeIII GGCC 4 cut(s) 483, 671, 1236, 1269
HapII CCGG 8 cut(s) 471, 558, 591, 645, 668, 672, 690, 726
HgaI GACGC 1 cut(s) 550
HhaI GCGC 5 cut(s) 425, 437, 596, 606, 640
Hin1I GRCGYC 3 cut(s) 543, 561, 594
Hin1II CATG 6 cut(s) 499, 586, 892, 980, 1026, 1164
Hin6I GCGC 5 cut(s) 423, 435, 594, 604, 638
HinP1I GCGC 5 cut(s) 423, 435, 594, 604, 638
HincII GTYRAC 2 cut(s) 550, 784
HindII GTYRAC 2 cut(s) 550, 784
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 2 cut(s) 882, 928
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 8 cut(s) 471, 558, 591, 645, 668, 672, 690, 726
HphI GGTGA 6 cut(s) 87, 119, 556, 670, 877, 1024
Hpy166II GTNNAC 4 cut(s) 328, 550, 784, 805
Hpy188I TCNGA 8 cut(s) 125, 134, 272, 322, 571, 1071, 1335, 1355
Hpy188III TCNNGA 3 cut(s) 382, 736, 938
Hpy8I GTNNAC 4 cut(s) 328, 550, 784, 805
Hpy99I CGWCG 4 cut(s) 268, 545, 548, 959
HpyAV CCTTC 4 cut(s) 1175, 1219, 1297, 1330
HpyCH4III ACNGT 2 cut(s) 403, 814
HpyCH4IV ACGT 3 cut(s) 543, 786, 957
HpyCH4V TGCA 6 cut(s) 304, 980, 1118, 1154, 1160, 1217
HpyF10VI GCNNNNNNNGC 7 cut(s) 417, 429, 480, 625, 1115, 1139, 1170
HpyF3I CTNAG 5 cut(s) 319, 867, 1008, 1083, 1248
HpySE526I ACGT 3 cut(s) 543, 786, 957
Hsp92I GRCGYC 3 cut(s) 543, 561, 594
Hsp92II CATG 6 cut(s) 499, 586, 892, 980, 1026, 1164
HspAI GCGC 5 cut(s) 423, 435, 594, 604, 638
KasI GGCGCC 1 cut(s) 593
KpnI GGTACC 1 cut(s) 852
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 660
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
LmnI GCTCC 4 cut(s) 464, 652, 1106, 1244
LweI GCATC 3 cut(s) 291, 1147, 1226
MaeI CTAG 3 cut(s) 348, 945, 1319
MaeII ACGT 3 cut(s) 543, 786, 957
MaeIII GTNAC 4 cut(s) 562, 883, 985, 1195
MalI GATC 9 cut(s) 170, 269, 274, 662, 743, 858, 862, 942, 1062
MboI GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
MboII GAAGA 4 cut(s) 211, 517, 855, 992
MfeI CAATTG 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 320, 681
MluCI AATT 7 cut(s) 100, 195, 276, 351, 415, 990, 1148
Mly113I GGCGCC 1 cut(s) 594
MlyI GAGTC 1 cut(s) 891
Mph1103I ATGCAT 1 cut(s) 1162
MroNI GCCGGC 1 cut(s) 470
MseI TTAA 4 cut(s) 500, 783, 1014, 1146
MslI CAYNNNNRTG 2 cut(s) 500, 1085
MspA1I CMGCKG 1 cut(s) 628
MspI CCGG 8 cut(s) 471, 558, 591, 645, 668, 672, 690, 726
MspR9I CCNGG 3 cut(s) 558, 690, 760
MunI CAATTG 1 cut(s) 100
Mva1269I GAATGC 2 cut(s) 1065, 1329
MvaI CCWGG 1 cut(s) 760
MvnI CGCG 3 cut(s) 332, 423, 606
MwoI GCNNNNNNNGC 7 cut(s) 417, 429, 480, 625, 1115, 1139, 1170
NaeI GCCGGC 1 cut(s) 472
NarI GGCGCC 1 cut(s) 594
NciI CCSGG 2 cut(s) 558, 690
NdeI CATATG 1 cut(s) 1135
NdeII GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
NgoMIV GCCGGC 1 cut(s) 470
NlaIII CATG 6 cut(s) 499, 586, 892, 980, 1026, 1164
NlaIV GGNNCC 4 cut(s) 369, 595, 850, 1270
NmuCI GTSAC 3 cut(s) 562, 883, 1195
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 1 cut(s) 1164
OliI CACNNNNGTG 1 cut(s) 1085
PaeI GCATGC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 2 cut(s) 960, 1174
PctI GAATGC 2 cut(s) 1065, 1329
PdiI GCCGGC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 928
PflFI GACNNNGTC 1 cut(s) 545
Ple19I CGATCG 1 cut(s) 270
PleI GAGTC 1 cut(s) 890
PluTI GGCGCC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 758
PspFI CCCAGC 2 cut(s) 201, 1187
PspGI CCWGG 1 cut(s) 758
PspN4I GGNNCC 4 cut(s) 369, 595, 850, 1270
PspPI GGNCC 2 cut(s) 367, 1268
PstNI CAGNNNCTG 2 cut(s) 628, 765
PsyI GACNNNGTC 1 cut(s) 545
PvuI CGATCG 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 2 cut(s) 850, 1240
RsaNI GTAC 2 cut(s) 849, 1239
RseI CAYNNNNRTG 2 cut(s) 500, 1085
SacI GAGCTC 1 cut(s) 320
SaqAI TTAA 4 cut(s) 500, 783, 1014, 1146
Sau3AI GATC 9 cut(s) 168, 267, 272, 660, 741, 856, 860, 940, 1060
Sau96I GGNCC 2 cut(s) 367, 1268
SchI GAGTC 1 cut(s) 891
ScrFI CCNGG 3 cut(s) 558, 690, 760
SduI GDGCHC 2 cut(s) 320, 681
SfaNI GCATC 3 cut(s) 291, 1147, 1226
SfoI GGCGCC 1 cut(s) 595
SgrAI CRCCGGYG 1 cut(s) 590
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 500, 1085
SphI GCATGC 1 cut(s) 1164
Sse9I AATT 7 cut(s) 100, 195, 276, 351, 415, 990, 1148
SsiI CCGC 9 cut(s) 105, 190, 357, 409, 421, 432, 474, 606, 1228
SspDI GGCGCC 1 cut(s) 593
SspMI CTAG 3 cut(s) 348, 945, 1319
SstI GAGCTC 1 cut(s) 320
StyD4I CCNGG 3 cut(s) 556, 688, 758
StyI CCWWGG 4 cut(s) 426, 516, 944, 1221
TaaI ACNGT 2 cut(s) 403, 814
TaiI ACGT 3 cut(s) 546, 789, 960
TaqI TCGA 5 cut(s) 48, 66, 263, 859, 954
TasI AATT 7 cut(s) 100, 195, 276, 351, 415, 990, 1148
TauI GCSGC 5 cut(s) 411, 423, 435, 477, 1231
TfiI GAWTC 1 cut(s) 928
Tru1I TTAA 4 cut(s) 500, 783, 1014, 1146
Tru9I TTAA 4 cut(s) 500, 783, 1014, 1146
TscAI CASTG 3 cut(s) 406, 751, 1087
TseFI GTSAC 3 cut(s) 562, 883, 1195
Tsp45I GTSAC 3 cut(s) 562, 883, 1195
TspDTI ATGAA 2 cut(s) 518, 524
TspGWI ACGGA 3 cut(s) 355, 514, 759
TspRI CASTG 3 cut(s) 406, 751, 1087
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 351, 1148
XceI RCATGY 1 cut(s) 1164
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmaJI CCTAGG 1 cut(s) 944
XspI CTAG 3 cut(s) 348, 945, 1319
ZraI GACGTC 1 cut(s) 544
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.