FvH4_1g18690

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014980
Physical Location & Seq
Forward (+)
10992665 .. 10997578
4914 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18690.t3

Sequence Viewer

Length: 1074 bp
ATGACACAGTCGAGCTCAGATGCCACTCAACGCATTGCTCGAATCTCCGCCCATCTCGACCCCCAAAATCTCCAGATGGACGATGCCGGAGCTCTCCGGCGACTGAGCTGCCGCGCCAAGGGTGGCGCGTCGGGCTTCAAAGTCGCGATTCTCGGCGCGGCCGGCGGTATAGGCCAGCCTCTGGCTTTGCTGATGAAGATGAACCCTCTCGTCTCCGTCCTCCATCTATACGACGTCGTTAACACCCCCGGCGTCACCTCTGACGTCAGCCACATGGACACCGGCGCCGTGGTGCGAGGGTTTCTAGGGCAGCAGCAGCTGGAGGAGGCTCTGACCGGAATGGAGCTGGTGATAATTCCGGCCGGTGTGCCTCGGAAACCGGGGATGACTAGGGATGATTTGTTTAACATCAATGCCGGAATTGTGAGGACGCTTTGTGAAGGAATTGCAAAGTGTTGTCCTAATGCTGTGGTTAACCTGATCAGCAATCCGGTGAACTCTACGGTTCCTATTGCTGCTGAGGTGTTCAAGAAAGCCGGGACTTTCGACCCGAAGCGGCTTTTGGGGGTTACTATGCTTGATGTTGTTAGGGCCAATACTTTTGTGGCAGAGGTTCTGGGACTTGATCCTAGGGAAGTTGATGTTCCTGTTGTTGGTGGTCATGCAGGGGTTACCATTTTGCCACTTCTGTCTCAGGTTAAACCCCCATGCTCTTTCACCCCGAAGGAAGTTGATTATCTAACTGATCGCATTCAGAATGGTGGAACTGAGGTTGTTGAGGCAAAAGCTGGAGCTGGTTCTGCAACATTATCAATGGCATATGCTGCTGTTAAATTTGCGGATGCATGCCTTCGTGGCTTAAGAGGGGATGCAGGTGTTGTTGAATGTGCATATGTCGCTTCTCAGGTGACTGAACTTCCCTTCTTTGCATCCAAAGTACGGCTTGGCCGTACTGGAGTTGAGGAAATACACCCCCTTGGCCCCCTGAATGAGTATGAGAGGGCTGGCTTGGAGAAGGCAAAGAAAGAACTAGCAACAAGTATACAGAAGGGGGTTTCTTTCATCAGAAAATGA

Protein Analysis

358

Amino Acids

37.64

Weight (kDa)

8.12

Isoelectric Point (pI)

28.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 47 - 189 3.8e-46 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 191 - 354 9.3e-46 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 863
AatII GACGTC 2 cut(s) 237, 267
Acc36I ACCTGC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 181
AccI GTMKAC 1 cut(s) 1042
AccII CGCG 4 cut(s) 114, 128, 146, 158
AciI CCGC 6 cut(s) 48, 112, 158, 165, 556, 839
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 3 cut(s) 159, 360, 946
AcsI RAATTY 1 cut(s) 833
AcyI GRCGYC 4 cut(s) 234, 252, 264, 285
AfaI GTAC 2 cut(s) 939, 952
AfiI CCNNNNNNNGG 4 cut(s) 118, 181, 653, 939
AflII CTTAAG 1 cut(s) 859
AgsI TTSAA 3 cut(s) 139, 529, 884
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 7 cut(s) 15, 92, 108, 319, 346, 788, 794
AluI AGCT 7 cut(s) 15, 92, 108, 319, 346, 788, 794
Alw21I GWGCWC 2 cut(s) 17, 94
Alw26I GTCTC 2 cut(s) 217, 696
AlwI GGATC 1 cut(s) 620
AlwNI CAGNNNCTG 2 cut(s) 181, 319
AoxI GGCC 6 cut(s) 159, 172, 360, 591, 946, 979
ApeKI GCWGC 6 cut(s) 108, 310, 313, 316, 515, 824
ApoI RAATTY 1 cut(s) 833
AspA2I CCTAGG 1 cut(s) 629
AspLEI GCGC 4 cut(s) 116, 128, 158, 287
AspS9I GGNCC 2 cut(s) 591, 980
AsuC2I CCSGG 3 cut(s) 249, 381, 538
AsuHPI GGTGA 5 cut(s) 247, 361, 505, 709, 919
AvrII CCTAGG 1 cut(s) 629
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 2 cut(s) 17, 94
Bbv12I GWGCWC 2 cut(s) 17, 94
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 6 cut(s) 95, 322, 325, 328, 502, 811
BccI CCATC 3 cut(s) 60, 70, 231
BceAI ACGGC 3 cut(s) 272, 933, 956
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 3 cut(s) 249, 381, 538
BcoDI GTCTC 2 cut(s) 217, 696
BfaI CTAG 4 cut(s) 305, 390, 630, 1031
BfoI RGCGCY 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 859
BfuAI ACCTGC 1 cut(s) 863
BglI GCCNNNNNGGC 1 cut(s) 855
BisI GCNGC 9 cut(s) 109, 112, 159, 311, 314, 317, 516, 557, 825
BlnI CCTAGG 1 cut(s) 629
BlsI GCNGC 9 cut(s) 110, 113, 160, 312, 315, 318, 517, 558, 826
Bme1390I CCNGG 3 cut(s) 249, 381, 538
BmgT120I GGNCC 2 cut(s) 591, 980
BmiI GGNNCC 3 cut(s) 286, 507, 982
BmrFI CCNGG 3 cut(s) 249, 381, 538
BmsI GCATC 5 cut(s) 10, 73, 832, 859, 938
BpmI CTGGAG 4 cut(s) 56, 341, 810, 975
Bpu10I CCTNAGC 1 cut(s) 519
BpuMI CCSGG 3 cut(s) 249, 381, 538
BsaHI GRCGYC 4 cut(s) 234, 252, 264, 285
BsaJI CCNNGG 7 cut(s) 117, 247, 288, 371, 380, 629, 976
BsaWI WCCGGW 2 cut(s) 335, 490
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 181, 653, 939
Bse118I RCCGGY 3 cut(s) 161, 281, 362
Bse1I ACTGG 1 cut(s) 958
Bse3DI GCAATG 1 cut(s) 33
BseDI CCNNGG 7 cut(s) 117, 247, 288, 371, 380, 629, 976
BseGI GGATG 5 cut(s) 390, 400, 847, 874, 929
BseLI CCNNNNNNNGG 4 cut(s) 118, 181, 653, 939
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 6 cut(s) 30, 95, 510, 707, 759, 917
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 1 cut(s) 338
BseX3I CGGCCG 2 cut(s) 159, 360
BseXI GCAGC 6 cut(s) 95, 322, 325, 328, 502, 811
Bsh1236I CGCG 4 cut(s) 114, 128, 146, 158
Bsh1285I CGRYCG 2 cut(s) 162, 363
BshFI GGCC 6 cut(s) 161, 174, 362, 593, 948, 981
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 2 cut(s) 162, 363
BsiHKAI GWGCWC 2 cut(s) 17, 94
BslFI GGGAC 2 cut(s) 553, 633
BslI CCNNNNNNNGG 4 cut(s) 118, 181, 653, 939
BsmAI GTCTC 2 cut(s) 217, 696
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 553, 633
BsmI GAATGC 1 cut(s) 750
BsnI GGCC 6 cut(s) 161, 174, 362, 593, 948, 981
Bsp1286I GDGCHC 2 cut(s) 17, 94
Bsp143I GATC 3 cut(s) 480, 625, 745
Bsp68I TCGCGA 1 cut(s) 146
BspACI CCGC 6 cut(s) 48, 112, 158, 165, 556, 839
BspANI GGCC 6 cut(s) 161, 174, 362, 593, 948, 981
BspCNI CTCAG 6 cut(s) 29, 96, 511, 706, 760, 916
BspFNI CGCG 4 cut(s) 114, 128, 146, 158
BspLI GGNNCC 3 cut(s) 286, 507, 982
BspMI ACCTGC 1 cut(s) 863
BspPI GGATC 1 cut(s) 620
BspT107I GGYRCC 1 cut(s) 284
BspTI CTTAAG 1 cut(s) 859
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 3 cut(s) 161, 281, 362
BsrI ACTGG 1 cut(s) 958
BssAI RCCGGY 3 cut(s) 161, 281, 362
BssECI CCNNGG 7 cut(s) 117, 247, 288, 371, 380, 629, 976
BssMI GATC 3 cut(s) 480, 625, 745
BssNAI GTATAC 1 cut(s) 1043
BssNI GRCGYC 4 cut(s) 234, 252, 264, 285
BssT1I CCWWGG 3 cut(s) 117, 629, 976
Bst1107I GTATAC 1 cut(s) 1043
Bst4CI ACNGT 2 cut(s) 9, 505
BstACI GRCGYC 4 cut(s) 234, 252, 264, 285
BstAFI CTTAAG 1 cut(s) 859
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 4 cut(s) 163, 176, 847, 1006
BstDEI CTNAG 6 cut(s) 16, 104, 519, 693, 768, 903
BstDSI CCRYGG 1 cut(s) 288
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 5 cut(s) 390, 400, 847, 874, 929
BstFNI CGCG 4 cut(s) 114, 128, 146, 158
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 4 cut(s) 116, 128, 158, 287
BstKTI GATC 3 cut(s) 483, 628, 748
BstMAI GTCTC 2 cut(s) 217, 696
BstMBI GATC 3 cut(s) 480, 625, 745
BstMCI CGRYCG 2 cut(s) 162, 363
BstMWI GCNNNNNNNGC 8 cut(s) 132, 162, 171, 316, 800, 824, 855, 896
BstNSI RCATGY 1 cut(s) 849
BstPI GGTNACC 1 cut(s) 670
BstSCI CCNGG 3 cut(s) 247, 379, 536
BstUI CGCG 4 cut(s) 114, 128, 146, 158
BstV1I GCAGC 6 cut(s) 95, 322, 325, 328, 502, 811
BstZ17I GTATAC 1 cut(s) 1043
BstZI CGGCCG 2 cut(s) 159, 360
BsuRI GGCC 6 cut(s) 161, 174, 362, 593, 948, 981
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 5 cut(s) 390, 400, 847, 874, 929
BtuMI TCGCGA 1 cut(s) 146
BveI ACCTGC 1 cut(s) 863
Cac8I GCNNGC 4 cut(s) 163, 176, 847, 1006
CaiI CAGNNNCTG 2 cut(s) 181, 319
CfoI GCGC 4 cut(s) 116, 128, 158, 287
Cfr10I RCCGGY 3 cut(s) 161, 281, 362
Cfr13I GGNCC 2 cut(s) 591, 980
CseI GACGC 3 cut(s) 117, 241, 439
Csp6I GTAC 2 cut(s) 938, 951
CviAII CATG 4 cut(s) 274, 662, 708, 846
CviQI GTAC 2 cut(s) 938, 951
DdeI CTNAG 6 cut(s) 16, 104, 519, 693, 768, 903
DinI GGCGCC 1 cut(s) 286
DpnI GATC 3 cut(s) 482, 627, 747
DpnII GATC 3 cut(s) 480, 625, 745
EaeI YGGCCR 3 cut(s) 159, 360, 946
EagI CGGCCG 2 cut(s) 159, 360
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 2 cut(s) 15, 92
EclXI CGGCCG 2 cut(s) 159, 360
Eco130I CCWWGG 3 cut(s) 117, 629, 976
Eco24I GRGCYC 2 cut(s) 17, 94
Eco52I CGGCCG 2 cut(s) 159, 360
Eco53kI GAGCTC 2 cut(s) 15, 92
Eco91I GGTNACC 1 cut(s) 670
EcoICRI GAGCTC 2 cut(s) 15, 92
EcoO65I GGTNACC 1 cut(s) 670
EcoT14I CCWWGG 3 cut(s) 117, 629, 976
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 2 cut(s) 17, 94
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 3 cut(s) 117, 629, 976
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 4 cut(s) 277, 665, 711, 849
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 2 cut(s) 553, 633
FatI CATG 4 cut(s) 273, 661, 707, 845
FauNDI CATATG 2 cut(s) 820, 892
FbaI TGATCA 1 cut(s) 480
FblI GTMKAC 1 cut(s) 1042
Fnu4HI GCNGC 9 cut(s) 109, 112, 159, 311, 314, 317, 516, 557, 825
FokI GGATG 5 cut(s) 397, 407, 854, 881, 916
FriOI GRGCYC 2 cut(s) 17, 94
Fsp4HI GCNGC 9 cut(s) 109, 112, 159, 311, 314, 317, 516, 557, 825
FspBI CTAG 4 cut(s) 305, 390, 630, 1031
GlaI GCGC 4 cut(s) 115, 127, 157, 286
GluI GCNGC 9 cut(s) 109, 112, 159, 311, 314, 317, 516, 557, 825
GsuI CTGGAG 4 cut(s) 56, 341, 810, 975
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 6 cut(s) 161, 174, 362, 593, 948, 981
HgaI GACGC 3 cut(s) 117, 241, 439
HhaI GCGC 4 cut(s) 116, 128, 158, 287
Hin1I GRCGYC 4 cut(s) 234, 252, 264, 285
Hin1II CATG 4 cut(s) 277, 665, 711, 849
Hin6I GCGC 4 cut(s) 114, 126, 156, 285
HinP1I GCGC 4 cut(s) 114, 126, 156, 285
HincII GTYRAC 2 cut(s) 241, 475
HindII GTYRAC 2 cut(s) 241, 475
HinfI GANTC 2 cut(s) 42, 148
HpaI GTTAAC 2 cut(s) 241, 475
HphI GGTGA 5 cut(s) 247, 361, 505, 709, 919
Hpy166II GTNNAC 4 cut(s) 241, 475, 496, 1043
Hpy188I TCNGA 6 cut(s) 19, 262, 333, 375, 756, 1067
Hpy188III TCNNGA 4 cut(s) 56, 73, 145, 529
Hpy8I GTNNAC 4 cut(s) 241, 475, 496, 1043
Hpy99I CGWCG 3 cut(s) 133, 236, 239
HpyAV CCTTC 6 cut(s) 434, 718, 860, 931, 1009, 1042
HpyCH4III ACNGT 2 cut(s) 9, 505
HpyCH4IV ACGT 2 cut(s) 234, 264
HpyCH4V TGCA 7 cut(s) 449, 665, 803, 845, 872, 890, 929
HpyF10VI GCNNNNNNNGC 8 cut(s) 132, 162, 171, 316, 800, 824, 855, 896
HpyF3I CTNAG 6 cut(s) 16, 104, 519, 693, 768, 903
HpySE526I ACGT 2 cut(s) 234, 264
Hsp92I GRCGYC 4 cut(s) 234, 252, 264, 285
Hsp92II CATG 4 cut(s) 277, 665, 711, 849
HspAI GCGC 4 cut(s) 114, 126, 156, 285
KasI GGCGCC 1 cut(s) 284
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 480
KspAI GTTAAC 2 cut(s) 241, 475
Kzo9I GATC 3 cut(s) 480, 625, 745
LmnI GCTCC 3 cut(s) 89, 343, 791
Lsp1109I GCAGC 6 cut(s) 95, 322, 325, 328, 502, 811
LweI GCATC 5 cut(s) 10, 73, 832, 859, 938
MaeI CTAG 4 cut(s) 305, 390, 630, 1031
MaeII ACGT 2 cut(s) 234, 264
MaeIII GTNAC 4 cut(s) 253, 568, 670, 907
MalI GATC 3 cut(s) 482, 627, 747
MboI GATC 3 cut(s) 480, 625, 745
MboII GAAGA 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 17, 94
MluCI AATT 4 cut(s) 354, 420, 444, 833
Mly113I GGCGCC 1 cut(s) 285
Mph1103I ATGCAT 1 cut(s) 847
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 6 cut(s) 240, 405, 474, 699, 831, 860
MspA1I CMGCKG 1 cut(s) 319
MspCI CTTAAG 1 cut(s) 859
MspR9I CCNGG 3 cut(s) 249, 381, 538
Mva1269I GAATGC 1 cut(s) 750
MvnI CGCG 4 cut(s) 114, 128, 146, 158
MwoI GCNNNNNNNGC 8 cut(s) 132, 162, 171, 316, 800, 824, 855, 896
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 3 cut(s) 249, 381, 538
NdeI CATATG 2 cut(s) 820, 892
NdeII GATC 3 cut(s) 480, 625, 745
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 4 cut(s) 277, 665, 711, 849
NlaIV GGNNCC 3 cut(s) 286, 507, 982
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 253, 907
NruI TCGCGA 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 1 cut(s) 849
PaeI GCATGC 1 cut(s) 849
PaqCI CACCTGC 1 cut(s) 863
PcsI WCGNNNNNNNCGW 2 cut(s) 37, 946
PctI GAATGC 1 cut(s) 750
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 42, 148
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 9 cut(s) 110, 113, 160, 312, 315, 318, 517, 558, 826
PluTI GGCGCC 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 17, 94
PspEI GGTNACC 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 286, 507, 982
PspPI GGNCC 2 cut(s) 591, 980
PstNI CAGNNNCTG 2 cut(s) 181, 319
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 319
RruI TCGCGA 1 cut(s) 146
RsaI GTAC 2 cut(s) 939, 952
RsaNI GTAC 2 cut(s) 938, 951
SacI GAGCTC 2 cut(s) 17, 94
SaqAI TTAA 6 cut(s) 240, 405, 474, 699, 831, 860
SatI GCNGC 9 cut(s) 109, 112, 159, 311, 314, 317, 516, 557, 825
Sau3AI GATC 3 cut(s) 480, 625, 745
Sau96I GGNCC 2 cut(s) 591, 980
ScrFI CCNGG 3 cut(s) 249, 381, 538
SduI GDGCHC 2 cut(s) 17, 94
SfaNI GCATC 5 cut(s) 10, 73, 832, 859, 938
SfoI GGCGCC 1 cut(s) 286
SgrAI CRCCGGYG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 859
SmoI CTYRAG 1 cut(s) 859
SphI GCATGC 1 cut(s) 849
Sse9I AATT 4 cut(s) 354, 420, 444, 833
SsiI CCGC 6 cut(s) 48, 112, 158, 165, 556, 839
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 4 cut(s) 305, 390, 630, 1031
SstI GAGCTC 2 cut(s) 17, 94
StyD4I CCNGG 3 cut(s) 247, 379, 536
StyI CCWWGG 3 cut(s) 117, 629, 976
TaaI ACNGT 2 cut(s) 9, 505
TaiI ACGT 2 cut(s) 237, 267
TaqI TCGA 4 cut(s) 11, 40, 57, 546
TasI AATT 4 cut(s) 354, 420, 444, 833
TauI GCSGC 3 cut(s) 114, 161, 559
TfiI GAWTC 2 cut(s) 42, 148
Tru1I TTAA 6 cut(s) 240, 405, 474, 699, 831, 860
Tru9I TTAA 6 cut(s) 240, 405, 474, 699, 831, 860
TseFI GTSAC 2 cut(s) 253, 907
TseI GCWGC 6 cut(s) 108, 310, 313, 316, 515, 824
Tsp45I GTSAC 2 cut(s) 253, 907
TspDTI ATGAA 3 cut(s) 209, 215, 1051
TspGWI ACGGA 1 cut(s) 205
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 181
Vha464I CTTAAG 1 cut(s) 859
XapI RAATTY 1 cut(s) 833
XceI RCATGY 1 cut(s) 849
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmaJI CCTAGG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 1042
XspI CTAG 4 cut(s) 305, 390, 630, 1031
ZraI GACGTC 2 cut(s) 235, 265
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.