Rh2BG223200

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014980
Physical Location & Seq
Forward (+)
21638390 .. 21640890
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG223200.1

Sequence Viewer

Length: 1071 bp
ATGCAGTCGAGCTCAGACGCGAGTCAACGGATTGCTAGAATCTCCGGCCATCTCGACCCTCAGAATCTCCAGATGGAGGACGCCGGGACTCTCCGGCGACTGGACTGCCGCGCCAAGGGCGGCGCGTCGGGTTTCAAAGTCGCGATTTTGGGAGCGGCCGGCGGTATAGGTCAGCCTCTGGCCATGTTGATGAAGATGAACCCTCTCGTCTCCGTCCTCCATCTATACGACGTCGTTAACACCCCCGGCGTCACCTCTGACATCAGCCACATGGACACCGGCGCCGTGGTGCGTGGGTTTTTAGGGCAGCAGCAGCTGGAGGAAGCTTTGATCGGAATGGAGCTGGTGATAATTCCGGCCGGCGTGCCTAGGAAACCGGGAATGACTAGAGATGATTTGTTTAACATCAATGCCGGAATTGTGAGGACGCTTTGTGAAGGAATTGCCAAGTGTTGTCCCAATGCTATAGTTAACTTGATTAGCAATCCTGTGAACTCTACGGTTCCTATTGCTGCTGAGGTTTTCAAAAGGGCTGGGACTTTCGACCCGAACCGGCTTTTGGGAGTTACCATGCTTGATGTTGTTAGAGCCAATACTTTTGTGGCAGAGGCTCTGGGTCTTGATCCTAGGGAGGTTGATGTCCCTGTTGTTGGTGGTCATGCTGGAGTTACTATTTTGCCTCTTCTGTCTCAGGTTAAGCCTCCATGCTCTTTCACCCCAAAGGAAGTTGATTATCTAACCGATCGCATTCAGAATGGTGGAACTGAGGTTGTTGAGGCAAAAGCTGGAGCTGGTTCTGCAACTTTATCAATGGCATACGCTGCTGTTAAATTTGCAGATGCATGCCTTCGTGGCTTAAGAGGGGACGCCGGTGTTGTTGAATGTGCATTTGTGGCTTCTCAGGTGACTGAACTTCCCTTCTTTGCATCGAAGGTACGGCTTGGCCGTACTGGAGTTGAGGAAATACACCCTCTTGGCCCCCTGAATGAGTATGAGAGGTCTGGTTTGGAGAAGGCAAAGAAAGAATTAGCAACAAGCATTCAGAAGGGGGTTTCTTTCATCAGAAAGTGA

Protein Analysis

356

Amino Acids

37.63

Weight (kDa)

6.96

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 46 - 188 2.6e-45 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 190 - 353 1.8e-45 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 155
AccII CGCG 4 cut(s) 20, 111, 125, 143
AciI CCGC 4 cut(s) 109, 120, 155, 162
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 5 cut(s) 46, 156, 180, 357, 943
AcsI RAATTY 1 cut(s) 830
AcyI GRCGYC 5 cut(s) 81, 231, 249, 282, 867
AfaI GTAC 2 cut(s) 936, 949
AfiI CCNNNNNNNGG 5 cut(s) 76, 100, 115, 559, 650
AflII CTTAAG 1 cut(s) 856
AgsI TTSAA 3 cut(s) 136, 526, 881
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AluBI AGCT 6 cut(s) 12, 316, 326, 343, 785, 791
AluI AGCT 6 cut(s) 12, 316, 326, 343, 785, 791
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 2 cut(s) 214, 693
AlwI GGATC 1 cut(s) 617
AlwNI CAGNNNCTG 2 cut(s) 178, 316
AoxI GGCC 6 cut(s) 46, 156, 180, 357, 943, 976
ApeKI GCWGC 5 cut(s) 307, 310, 313, 512, 821
ApoI RAATTY 1 cut(s) 830
AspA2I CCTAGG 2 cut(s) 368, 626
AspLEI GCGC 3 cut(s) 113, 125, 284
AspS9I GGNCC 1 cut(s) 977
AsuC2I CCSGG 3 cut(s) 85, 246, 378
AsuHPI GGTGA 4 cut(s) 244, 358, 706, 916
AvrII CCTAGG 2 cut(s) 368, 626
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 5 cut(s) 319, 322, 325, 499, 808
BccI CCATC 3 cut(s) 57, 67, 228
BceAI ACGGC 3 cut(s) 269, 930, 953
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BcnI CCSGG 3 cut(s) 85, 246, 378
BcoDI GTCTC 2 cut(s) 214, 693
BfaI CTAG 4 cut(s) 36, 369, 387, 627
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 285
BfrI CTTAAG 1 cut(s) 856
BglI GCCNNNNNGGC 1 cut(s) 852
BisI GCNGC 8 cut(s) 109, 121, 156, 308, 311, 314, 513, 822
BlnI CCTAGG 2 cut(s) 368, 626
BlsI GCNGC 8 cut(s) 110, 122, 157, 309, 312, 315, 514, 823
Bme1390I CCNGG 3 cut(s) 85, 246, 378
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 3 cut(s) 283, 504, 979
BmrFI CCNGG 3 cut(s) 85, 246, 378
BmsI GCATC 2 cut(s) 829, 935
BoxI GACNNNNGTC 1 cut(s) 21
BpmI CTGGAG 5 cut(s) 53, 338, 684, 807, 972
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 3 cut(s) 85, 246, 378
BsaHI GRCGYC 5 cut(s) 81, 231, 249, 282, 867
BsaJI CCNNGG 5 cut(s) 114, 244, 285, 368, 626
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 100, 115, 559, 650
Bse118I RCCGGY 5 cut(s) 158, 278, 359, 552, 869
Bse1I ACTGG 2 cut(s) 105, 955
BseDI CCNNGG 5 cut(s) 114, 244, 285, 368, 626
BseLI CCNNNNNNNGG 5 cut(s) 76, 100, 115, 559, 650
BseMII CTCAG 6 cut(s) 27, 74, 507, 704, 756, 914
BseNI ACTGG 2 cut(s) 105, 955
BseX3I CGGCCG 2 cut(s) 156, 357
BseXI GCAGC 5 cut(s) 319, 322, 325, 499, 808
BseYI CCCAGC 1 cut(s) 533
Bsh1236I CGCG 4 cut(s) 20, 111, 125, 143
Bsh1285I CGRYCG 3 cut(s) 159, 360, 745
BshFI GGCC 6 cut(s) 48, 158, 182, 359, 945, 978
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 3 cut(s) 159, 360, 745
BsiHKAI GWGCWC 1 cut(s) 14
BslFI GGGAC 5 cut(s) 100, 441, 550, 626, 878
BslI CCNNNNNNNGG 5 cut(s) 76, 100, 115, 559, 650
BsmAI GTCTC 2 cut(s) 214, 693
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 5 cut(s) 100, 441, 550, 626, 878
BsmI GAATGC 2 cut(s) 747, 1038
BsnI GGCC 6 cut(s) 48, 158, 182, 359, 945, 978
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 3 cut(s) 330, 622, 742
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 4 cut(s) 109, 120, 155, 162
BspANI GGCC 6 cut(s) 48, 158, 182, 359, 945, 978
BspCNI CTCAG 6 cut(s) 26, 73, 508, 703, 757, 913
BspFNI CGCG 4 cut(s) 20, 111, 125, 143
BspLI GGNNCC 3 cut(s) 283, 504, 979
BspPI GGATC 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 281
BspTI CTTAAG 1 cut(s) 856
BsrBI CCGCTC 1 cut(s) 155
BsrFI RCCGGY 5 cut(s) 158, 278, 359, 552, 869
BsrI ACTGG 2 cut(s) 105, 955
BssAI RCCGGY 5 cut(s) 158, 278, 359, 552, 869
BssECI CCNNGG 5 cut(s) 114, 244, 285, 368, 626
BssMI GATC 3 cut(s) 330, 622, 742
BssNI GRCGYC 5 cut(s) 81, 231, 249, 282, 867
BssT1I CCWWGG 3 cut(s) 114, 368, 626
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 5 cut(s) 81, 231, 249, 282, 867
BstAFI CTTAAG 1 cut(s) 856
BstAPI GCANNNNNTGC 1 cut(s) 821
BstC8I GCNNGC 4 cut(s) 160, 361, 365, 844
BstDEI CTNAG 6 cut(s) 13, 60, 516, 690, 765, 900
BstDSI CCRYGG 1 cut(s) 285
BstFNI CGCG 4 cut(s) 20, 111, 125, 143
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 3 cut(s) 113, 125, 284
BstKTI GATC 3 cut(s) 333, 625, 745
BstMAI GTCTC 2 cut(s) 214, 693
BstMBI GATC 3 cut(s) 330, 622, 742
BstMCI CGRYCG 3 cut(s) 159, 360, 745
BstMWI GCNNNNNNNGC 6 cut(s) 117, 313, 797, 821, 852, 893
BstNSI RCATGY 1 cut(s) 846
BstPAI GACNNNNGTC 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 83, 244, 376
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 4 cut(s) 20, 111, 125, 143
BstV1I GCAGC 5 cut(s) 319, 322, 325, 499, 808
BstZI CGGCCG 2 cut(s) 156, 357
BsuRI GGCC 6 cut(s) 48, 158, 182, 359, 945, 978
BtgI CCRYGG 1 cut(s) 285
BtuMI TCGCGA 1 cut(s) 143
Cac8I GCNNGC 4 cut(s) 160, 361, 365, 844
CaiI CAGNNNCTG 2 cut(s) 178, 316
CfoI GCGC 3 cut(s) 113, 125, 284
Cfr10I RCCGGY 5 cut(s) 158, 278, 359, 552, 869
Cfr13I GGNCC 1 cut(s) 977
CseI GACGC 6 cut(s) 26, 89, 114, 238, 436, 875
Csp6I GTAC 2 cut(s) 935, 948
CviAII CATG 6 cut(s) 184, 271, 571, 659, 705, 843
CviQI GTAC 2 cut(s) 935, 948
DdeI CTNAG 6 cut(s) 13, 60, 516, 690, 765, 900
DinI GGCGCC 1 cut(s) 283
DpnI GATC 3 cut(s) 332, 624, 744
DpnII GATC 3 cut(s) 330, 622, 742
EaeI YGGCCR 5 cut(s) 46, 156, 180, 357, 943
EagI CGGCCG 2 cut(s) 156, 357
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 12
EclXI CGGCCG 2 cut(s) 156, 357
Eco130I CCWWGG 3 cut(s) 114, 368, 626
Eco24I GRGCYC 1 cut(s) 14
Eco52I CGGCCG 2 cut(s) 156, 357
Eco53kI GAGCTC 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 12
EcoT14I CCWWGG 3 cut(s) 114, 368, 626
EcoT22I ATGCAT 1 cut(s) 844
EcoT38I GRGCYC 1 cut(s) 14
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 3 cut(s) 114, 368, 626
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 6 cut(s) 187, 274, 574, 662, 708, 846
FaqI GGGAC 5 cut(s) 100, 441, 550, 626, 878
FatI CATG 6 cut(s) 183, 270, 570, 658, 704, 842
Fnu4HI GCNGC 8 cut(s) 109, 121, 156, 308, 311, 314, 513, 822
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 8 cut(s) 109, 121, 156, 308, 311, 314, 513, 822
FspBI CTAG 4 cut(s) 36, 369, 387, 627
GlaI GCGC 3 cut(s) 112, 124, 283
GluI GCNGC 8 cut(s) 109, 121, 156, 308, 311, 314, 513, 822
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 5 cut(s) 53, 338, 684, 807, 972
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 6 cut(s) 48, 158, 182, 359, 945, 978
HgaI GACGC 6 cut(s) 26, 89, 114, 238, 436, 875
HhaI GCGC 3 cut(s) 113, 125, 284
Hin1I GRCGYC 5 cut(s) 81, 231, 249, 282, 867
Hin1II CATG 6 cut(s) 187, 274, 574, 662, 708, 846
Hin6I GCGC 3 cut(s) 111, 123, 282
HinP1I GCGC 3 cut(s) 111, 123, 282
HincII GTYRAC 3 cut(s) 26, 238, 472
HindII GTYRAC 3 cut(s) 26, 238, 472
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 4 cut(s) 22, 39, 64, 88
HpaI GTTAAC 2 cut(s) 238, 472
HphI GGTGA 4 cut(s) 244, 358, 706, 916
Hpy166II GTNNAC 4 cut(s) 26, 238, 472, 493
Hpy188I TCNGA 7 cut(s) 16, 63, 259, 335, 753, 1044, 1064
Hpy188III TCNNGA 4 cut(s) 53, 70, 142, 620
Hpy8I GTNNAC 4 cut(s) 26, 238, 472, 493
Hpy99I CGWCG 3 cut(s) 130, 233, 236
HpyAV CCTTC 6 cut(s) 431, 857, 925, 928, 1006, 1039
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 6 cut(s) 4, 800, 836, 842, 887, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 313, 797, 821, 852, 893
HpyF3I CTNAG 6 cut(s) 13, 60, 516, 690, 765, 900
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 5 cut(s) 81, 231, 249, 282, 867
Hsp92II CATG 6 cut(s) 187, 274, 574, 662, 708, 846
HspAI GCGC 3 cut(s) 111, 123, 282
KasI GGCGCC 1 cut(s) 281
KroI GCCGGC 2 cut(s) 158, 359
KroNI GCCGGC 2 cut(s) 160, 361
KspAI GTTAAC 2 cut(s) 238, 472
Kzo9I GATC 3 cut(s) 330, 622, 742
LmnI GCTCC 3 cut(s) 152, 340, 788
Lsp1109I GCAGC 5 cut(s) 319, 322, 325, 499, 808
LweI GCATC 2 cut(s) 829, 935
MaeI CTAG 4 cut(s) 36, 369, 387, 627
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 4 cut(s) 250, 565, 667, 904
MalI GATC 3 cut(s) 332, 624, 744
MbiI CCGCTC 1 cut(s) 155
MboI GATC 3 cut(s) 330, 622, 742
MboII GAAGA 2 cut(s) 205, 674
MhlI GDGCHC 1 cut(s) 14
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 5 cut(s) 351, 417, 441, 830, 1025
MluNI TGGCCA 1 cut(s) 182
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 2 cut(s) 31, 82
Mox20I TGGCCA 1 cut(s) 182
Mph1103I ATGCAT 1 cut(s) 844
MroNI GCCGGC 2 cut(s) 158, 359
MscI TGGCCA 1 cut(s) 182
MseI TTAA 6 cut(s) 237, 402, 471, 696, 828, 857
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 316
MspCI CTTAAG 1 cut(s) 856
MspR9I CCNGG 3 cut(s) 85, 246, 378
Mva1269I GAATGC 2 cut(s) 747, 1038
MvnI CGCG 4 cut(s) 20, 111, 125, 143
MwoI GCNNNNNNNGC 6 cut(s) 117, 313, 797, 821, 852, 893
NaeI GCCGGC 2 cut(s) 160, 361
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 3 cut(s) 85, 246, 378
NdeII GATC 3 cut(s) 330, 622, 742
NgoMIV GCCGGC 2 cut(s) 158, 359
NlaIII CATG 6 cut(s) 187, 274, 574, 662, 708, 846
NlaIV GGNNCC 3 cut(s) 283, 504, 979
NmuCI GTSAC 2 cut(s) 250, 904
NruI TCGCGA 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 844
NspI RCATGY 1 cut(s) 846
PaeI GCATGC 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 943
PctI GAATGC 2 cut(s) 747, 1038
PdiI GCCGGC 2 cut(s) 160, 361
PfeI GAWTC 2 cut(s) 39, 64
PkrI GCNGC 8 cut(s) 110, 122, 157, 309, 312, 315, 514, 823
Ple19I CGATCG 1 cut(s) 745
PleI GAGTC 2 cut(s) 30, 82
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 2 cut(s) 30, 82
PshAI GACNNNNGTC 1 cut(s) 21
Psp124BI GAGCTC 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 533
PspN4I GGNNCC 3 cut(s) 283, 504, 979
PspPI GGNCC 1 cut(s) 977
PstNI CAGNNNCTG 2 cut(s) 178, 316
PvuI CGATCG 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 316
RruI TCGCGA 1 cut(s) 143
RsaI GTAC 2 cut(s) 936, 949
RsaNI GTAC 2 cut(s) 935, 948
RseI CAYNNNNRTG 1 cut(s) 188
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 6 cut(s) 237, 402, 471, 696, 828, 857
SatI GCNGC 8 cut(s) 109, 121, 156, 308, 311, 314, 513, 822
Sau3AI GATC 3 cut(s) 330, 622, 742
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 2 cut(s) 31, 82
ScrFI CCNGG 3 cut(s) 85, 246, 378
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 2 cut(s) 829, 935
SfcI CTRYAG 1 cut(s) 465
SfoI GGCGCC 1 cut(s) 283
SgrAI CRCCGGYG 2 cut(s) 278, 869
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 856
SmoI CTYRAG 1 cut(s) 856
SphI GCATGC 1 cut(s) 846
Sse9I AATT 5 cut(s) 351, 417, 441, 830, 1025
SsiI CCGC 4 cut(s) 109, 120, 155, 162
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 4 cut(s) 36, 369, 387, 627
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 83, 244, 376
StyI CCWWGG 3 cut(s) 114, 368, 626
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 1 cut(s) 234
TaqI TCGA 4 cut(s) 8, 54, 543, 929
TasI AATT 5 cut(s) 351, 417, 441, 830, 1025
TauI GCSGC 3 cut(s) 111, 123, 158
TfiI GAWTC 2 cut(s) 39, 64
Tru1I TTAA 6 cut(s) 237, 402, 471, 696, 828, 857
Tru9I TTAA 6 cut(s) 237, 402, 471, 696, 828, 857
TseFI GTSAC 2 cut(s) 250, 904
TseI GCWGC 5 cut(s) 307, 310, 313, 512, 821
Tsp45I GTSAC 2 cut(s) 250, 904
TspDTI ATGAA 3 cut(s) 206, 212, 1048
TspGWI ACGGA 2 cut(s) 43, 202
Vha464I CTTAAG 1 cut(s) 856
XapI RAATTY 1 cut(s) 830
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XmaJI CCTAGG 2 cut(s) 368, 626
XspI CTAG 4 cut(s) 36, 369, 387, 627
ZraI GACGTC 1 cut(s) 232
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.