Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014980

Physical Location & Seq

Genomic Coordinates Forward (+)

29689886 .. 29693511

Gene Structure 3626 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1304800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGCAGCAGCATCCTAGCTCAGAAGTAAACGCGAATCAACGGATTGCTAGAATCTCCGCCCATCTGGACCCTCCAAATCTCCAGATGGAGAACGCTGCCACTGTTAACCGCTTGAATTGCCGCGCCAAAGGTGGTACCGCTGGTTACAAAGTAGCCATACTCGGAGCAGCCGGCGGCATAGGCCAGCCTCTCGCCATGTTGATGAAGATGAACCCTTTGGTCTCCGTTCTCCACCTATACGACGTCGTCAACACCCCCGGCGTTACCTCCGATATCAGCCACATGGACACCGGCGCCGTCGTGCGCGGGTTTTTGGGGCAGCAGCAGCTGGACGAAGCGCTGACCGGAATGGAACTGGTGATCATCCCGGCCGGCGTGCCTCGAAAACCCGGAATGACAAGGGACGATTTGTTTAACATCAATGCCGGAATTGTCAAGACGATCAGTGAAGCAATCGCCAGGTGCTGTCCGTACGCCATTGTTAACGTGATTAGCAACCCTGTGAACTCCACTGTTCCCATTGCTGCTGAAGTTTTAAAAAAAGCCGGTACCTTTGACCCTAAGAAACTGTTGGGAGTTACCATGCTTGATGTTGTTAGAGCCAATACTTTTGTGGCAGAGGTTTTGGGACTTGATCCTAGGGATGTCGGTGTTCCCGTTGTTGGGGGTCATGCAGGGGTTACAATTTTGCCTCTTCTGTCTCAGGTTAAGCCTCCATGTTCTTTCACCCCGAAAGAAGTTGATGATCTAACATATCGCATTCAGAATGGTGGGACTGAGGTTGTGGAGGCAAAAGCTGGAGCTGGTTCTGCAACATTATCAATGGCATATGCTGCTGTTAAATTTGCAGATGCATGCCTCCGAGGCTTGCGAGGAGATGCTGGCGTAGTCGAATGTGCATTTGTGGCTTCTCAGGTTACTGAACTTCCCTTCTTTGCATCCAAGGTACGGCTTGGCCGTACTGGAGCTGAGGAAATATACCCTCTTGGTCCCCTGAATGAGTATGAGAGGGCCGGCTTGGAGAAGGCAAAGAGAGAACTAGCAACAAGCATTCAGAAGGGGGTTTCTTTCGTCAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0009514 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

361

Amino Acids

38.01

Weight (kDa)

8.46

Isoelectric Point (pI)

25.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	50 - 192	1.5e-45	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	194 - 357	2.9e-43	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22780
fragaria_vesca	FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica	MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica	Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis	pycom02g15840 pycom15g26720
rosa_chinensis	RchiOBHm_Chr2g0109561
rosa_laevigata	RLG00000017745
rosa_multiflora	Rmu_sc0000332.1_g000042
rosa_roxburghii	Rroxscaffold_2G00134150
rosa_rugosa	Rorug02G0160600
rosa_samantha	Rh2BG223200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	246
Acc65I	GGTACC	2 cut(s)	134, 548
AccB1I	GGYRCC	3 cut(s)	134, 293, 548
AccII	CGCG	3 cut(s)	32, 123, 306
AciI	CCGC	6 cut(s)	57, 109, 121, 138, 174, 306
AclWI	GGATC	1 cut(s)	629
AcoI	YGGCCR	2 cut(s)	369, 955
AcsI	RAATTY	1 cut(s)	842
AcuI	CTGAAG	1 cut(s)	549
AcyI	GRCGYC	2 cut(s)	243, 294
AfaI	GTAC	5 cut(s)	136, 473, 550, 948, 961
AfeI	AGCGCT	1 cut(s)	339
AfiI	CCNNNNNNNGG	3 cut(s)	662, 663, 948
AgsI	TTSAA	1 cut(s)	115
AjnI	CCWGG	1 cut(s)	458
AluBI	AGCT	5 cut(s)	18, 328, 797, 803, 968
AluI	AGCT	5 cut(s)	18, 328, 797, 803, 968
Alw26I	GTCTC	2 cut(s)	226, 705
AlwI	GGATC	1 cut(s)	629
AlwNI	CAGNNNCTG	2 cut(s)	328, 465
Aor51HI	AGCGCT	1 cut(s)	339
AoxI	GGCC	4 cut(s)	181, 369, 955, 1011
ApeKI	GCWGC	9 cut(s)	4, 7, 95, 167, 319, 322, 325, 524, 833
ApoI	RAATTY	1 cut(s)	842
Asp718I	GGTACC	2 cut(s)	134, 548
AspA2I	CCTAGG	1 cut(s)	638
AspLEI	GCGC	4 cut(s)	125, 296, 306, 340
AspS9I	GGNCC	3 cut(s)	67, 989, 1011
AsuC2I	CCSGG	3 cut(s)	258, 368, 390
AsuHPI	GGTGA	2 cut(s)	370, 718
AvaII	GGWCC	2 cut(s)	67, 989
AvrII	CCTAGG	1 cut(s)	638
BanI	GGYRCC	3 cut(s)	134, 293, 548
BbvCI	CCTCAGC	1 cut(s)	969
BbvI	GCAGC	9 cut(s)	16, 19, 82, 179, 331, 334, 337, 511, 820
BccI	CCATC	2 cut(s)	69, 79
BceAI	ACGGC	3 cut(s)	281, 942, 965
BciT130I	CCWGG	1 cut(s)	460
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	3 cut(s)	258, 368, 390
BcoDI	GTCTC	2 cut(s)	226, 705
BfaI	CTAG	4 cut(s)	15, 48, 639, 1040
BfoI	RGCGCY	2 cut(s)	297, 341
BglI	GCCNNNNNGGC	1 cut(s)	864
BlnI	CCTAGG	1 cut(s)	638
Bme1390I	CCNGG	4 cut(s)	258, 368, 390, 460
Bme18I	GGWCC	2 cut(s)	67, 989
BmgT120I	GGNCC	3 cut(s)	67, 989, 1011
BmiI	GGNNCC	5 cut(s)	69, 136, 295, 550, 991
BmrFI	CCNGG	4 cut(s)	258, 368, 390, 460
BmsI	GCATC	4 cut(s)	19, 841, 868, 947
BpmI	CTGGAG	3 cut(s)	65, 819, 984
Bpu10I	CCTNAGC	1 cut(s)	969
BpuMI	CCSGG	3 cut(s)	258, 368, 390
BsaHI	GRCGYC	2 cut(s)	243, 294
BsaI	GGTCTC	1 cut(s)	226
BsaJI	CCNNGG	4 cut(s)	256, 638, 862, 942
BsaWI	WCCGGW	1 cut(s)	344
Bsc4I	CCNNNNNNNGG	3 cut(s)	662, 663, 948
Bse118I	RCCGGY	5 cut(s)	170, 290, 371, 545, 1013
Bse1I	ACTGG	2 cut(s)	360, 967
Bse3DI	GCAATG	1 cut(s)	519
BseBI	CCWGG	1 cut(s)	460
BseDI	CCNNGG	4 cut(s)	256, 638, 862, 942
BseGI	GGATG	4 cut(s)	10, 363, 649, 938
BseLI	CCNNNNNNNGG	3 cut(s)	662, 663, 948
BseMI	GCAATG	1 cut(s)	519
BseMII	CTCAG	5 cut(s)	33, 716, 768, 926, 960
BseNI	ACTGG	2 cut(s)	360, 967
BseRI	GAGGAG	1 cut(s)	888
BseX3I	CGGCCG	1 cut(s)	369
BseXI	GCAGC	9 cut(s)	16, 19, 82, 179, 331, 334, 337, 511, 820
Bsh1236I	CGCG	3 cut(s)	32, 123, 306
Bsh1285I	CGRYCG	1 cut(s)	372
BshFI	GGCC	4 cut(s)	183, 371, 957, 1013
BshNI	GGYRCC	3 cut(s)	134, 293, 548
BsiEI	CGRYCG	1 cut(s)	372
BsiWI	CGTACG	1 cut(s)	471
BslFI	GGGAC	4 cut(s)	416, 642, 787, 975
BslI	CCNNNNNNNGG	3 cut(s)	662, 663, 948
BsmAI	GTCTC	2 cut(s)	226, 705
BsmFI	GGGAC	4 cut(s)	416, 642, 787, 975
BsmI	GAATGC	2 cut(s)	759, 1050
BsnI	GGCC	4 cut(s)	183, 371, 957, 1013
Bso31I	GGTCTC	1 cut(s)	226
Bsp143I	GATC	4 cut(s)	360, 441, 634, 745
BspACI	CCGC	6 cut(s)	57, 109, 121, 138, 174, 306
BspANI	GGCC	4 cut(s)	183, 371, 957, 1013
BspCNI	CTCAG	5 cut(s)	32, 715, 769, 925, 961
BspFNI	CGCG	3 cut(s)	32, 123, 306
BspLI	GGNNCC	5 cut(s)	69, 136, 295, 550, 991
BspPI	GGATC	1 cut(s)	629
BspT107I	GGYRCC	3 cut(s)	134, 293, 548
BspTNI	GGTCTC	1 cut(s)	226
BsrDI	GCAATG	1 cut(s)	519
BsrFI	RCCGGY	5 cut(s)	170, 290, 371, 545, 1013
BsrI	ACTGG	2 cut(s)	360, 967
BssAI	RCCGGY	5 cut(s)	170, 290, 371, 545, 1013
BssECI	CCNNGG	4 cut(s)	256, 638, 862, 942
BssMI	GATC	4 cut(s)	360, 441, 634, 745
BssNI	GRCGYC	2 cut(s)	243, 294
BssT1I	CCWWGG	2 cut(s)	638, 942
Bst2UI	CCWGG	1 cut(s)	460
Bst4CI	ACNGT	3 cut(s)	103, 514, 570
Bst6I	CTCTTC	1 cut(s)	699
BstACI	GRCGYC	2 cut(s)	243, 294
BstAPI	GCANNNNNTGC	1 cut(s)	833
BstC8I	GCNNGC	8 cut(s)	172, 185, 373, 377, 856, 869, 883, 1015
BstDEI	CTNAG	6 cut(s)	19, 561, 702, 777, 912, 969
BstF5I	GGATG	4 cut(s)	10, 363, 649, 938
BstFNI	CGCG	3 cut(s)	32, 123, 306
BstH2I	RGCGCY	2 cut(s)	297, 341
BstHHI	GCGC	4 cut(s)	125, 296, 306, 340
BstKTI	GATC	4 cut(s)	363, 444, 637, 748
BstMAI	GTCTC	2 cut(s)	226, 705
BstMBI	GATC	4 cut(s)	360, 441, 634, 745
BstMCI	CGRYCG	1 cut(s)	372
BstMWI	GCNNNNNNNGC	7 cut(s)	117, 180, 325, 809, 833, 864, 905
BstNI	CCWGG	1 cut(s)	460
BstNSI	RCATGY	1 cut(s)	858
BstSCI	CCNGG	4 cut(s)	256, 366, 388, 458
BstUI	CGCG	3 cut(s)	32, 123, 306
BstV1I	GCAGC	9 cut(s)	16, 19, 82, 179, 331, 334, 337, 511, 820
BstZI	CGGCCG	1 cut(s)	369
BsuRI	GGCC	4 cut(s)	183, 371, 957, 1013
BtsCI	GGATG	4 cut(s)	10, 363, 649, 938
BtsIMutI	CAGTG	3 cut(s)	99, 451, 510
Cac8I	GCNNGC	8 cut(s)	172, 185, 373, 377, 856, 869, 883, 1015
CaiI	CAGNNNCTG	2 cut(s)	328, 465
CfoI	GCGC	4 cut(s)	125, 296, 306, 340
Cfr10I	RCCGGY	5 cut(s)	170, 290, 371, 545, 1013
Cfr13I	GGNCC	3 cut(s)	67, 989, 1011
Csp6I	GTAC	5 cut(s)	135, 472, 549, 947, 960
CviAII	CATG	6 cut(s)	196, 283, 583, 671, 717, 855
CviQI	GTAC	5 cut(s)	135, 472, 549, 947, 960
DdeI	CTNAG	6 cut(s)	19, 561, 702, 777, 912, 969
DinI	GGCGCC	1 cut(s)	295
DpnI	GATC	4 cut(s)	362, 443, 636, 747
DpnII	GATC	4 cut(s)	360, 441, 634, 745
DraI	TTTAAA	1 cut(s)	537
EaeI	YGGCCR	2 cut(s)	369, 955
EagI	CGGCCG	1 cut(s)	369
Eam1104I	CTCTTC	1 cut(s)	699
EarI	CTCTTC	1 cut(s)	699
EciI	GGCGGA	1 cut(s)	46
EclXI	CGGCCG	1 cut(s)	369
Eco130I	CCWWGG	2 cut(s)	638, 942
Eco31I	GGTCTC	1 cut(s)	226
Eco32I	GATATC	1 cut(s)	274
Eco47I	GGWCC	2 cut(s)	67, 989
Eco47III	AGCGCT	1 cut(s)	339
Eco52I	CGGCCG	1 cut(s)	369
Eco57I	CTGAAG	1 cut(s)	549
EcoRII	CCWGG	1 cut(s)	458
EcoRV	GATATC	1 cut(s)	274
EcoT14I	CCWWGG	2 cut(s)	638, 942
EcoT22I	ATGCAT	1 cut(s)	856
EgeI	GGCGCC	1 cut(s)	295
EheI	GGCGCC	1 cut(s)	295
ErhI	CCWWGG	2 cut(s)	638, 942
FaeI	CATG	6 cut(s)	199, 286, 586, 674, 720, 858
FaqI	GGGAC	4 cut(s)	416, 642, 787, 975
FatI	CATG	6 cut(s)	195, 282, 582, 670, 716, 854
FauI	CCCGC	1 cut(s)	299
FauNDI	CATATG	1 cut(s)	829
FbaI	TGATCA	1 cut(s)	360
FokI	GGATG	3 cut(s)	350, 656, 925
FspBI	CTAG	4 cut(s)	15, 48, 639, 1040
GlaI	GCGC	4 cut(s)	124, 295, 305, 339
GsuI	CTGGAG	3 cut(s)	65, 819, 984
HaeII	RGCGCY	2 cut(s)	297, 341
HaeIII	GGCC	4 cut(s)	183, 371, 957, 1013
HhaI	GCGC	4 cut(s)	125, 296, 306, 340
Hin1I	GRCGYC	2 cut(s)	243, 294
Hin1II	CATG	6 cut(s)	199, 286, 586, 674, 720, 858
Hin6I	GCGC	4 cut(s)	123, 294, 304, 338
HinP1I	GCGC	4 cut(s)	123, 294, 304, 338
HincII	GTYRAC	3 cut(s)	106, 250, 484
HindII	GTYRAC	3 cut(s)	106, 250, 484
HinfI	GANTC	2 cut(s)	34, 51
HpaI	GTTAAC	2 cut(s)	106, 484
HphI	GGTGA	2 cut(s)	370, 718
Hpy166II	GTNNAC	5 cut(s)	28, 106, 250, 484, 505
Hpy188I	TCNGA	7 cut(s)	22, 164, 271, 765, 863, 1056, 1076
Hpy188III	TCNNGA	3 cut(s)	65, 82, 436
Hpy8I	GTNNAC	5 cut(s)	28, 106, 250, 484, 505
Hpy99I	CGWCG	3 cut(s)	245, 248, 302
HpyAV	CCTTC	3 cut(s)	940, 1018, 1051
HpyCH4III	ACNGT	3 cut(s)	103, 514, 570
HpyCH4IV	ACGT	2 cut(s)	243, 486
HpyCH4V	TGCA	7 cut(s)	4, 674, 812, 848, 854, 899, 938
HpyF10VI	GCNNNNNNNGC	7 cut(s)	117, 180, 325, 809, 833, 864, 905
HpyF3I	CTNAG	6 cut(s)	19, 561, 702, 777, 912, 969
HpySE526I	ACGT	2 cut(s)	243, 486
Hsp92I	GRCGYC	2 cut(s)	243, 294
Hsp92II	CATG	6 cut(s)	199, 286, 586, 674, 720, 858
HspAI	GCGC	4 cut(s)	123, 294, 304, 338
KasI	GGCGCC	1 cut(s)	293
KpnI	GGTACC	2 cut(s)	138, 552
KroI	GCCGGC	3 cut(s)	170, 371, 1013
KroNI	GCCGGC	3 cut(s)	172, 373, 1015
Ksp22I	TGATCA	1 cut(s)	360
KspAI	GTTAAC	2 cut(s)	106, 484
Kzo9I	GATC	4 cut(s)	360, 441, 634, 745
LmnI	GCTCC	3 cut(s)	164, 800, 965
Lsp1109I	GCAGC	9 cut(s)	16, 19, 82, 179, 331, 334, 337, 511, 820
LweI	GCATC	4 cut(s)	19, 841, 868, 947
MaeI	CTAG	4 cut(s)	15, 48, 639, 1040
MaeII	ACGT	2 cut(s)	243, 486
MaeIII	GTNAC	5 cut(s)	143, 262, 577, 679, 916
MalI	GATC	4 cut(s)	362, 443, 636, 747
MboI	GATC	4 cut(s)	360, 441, 634, 745
MboII	GAAGA	2 cut(s)	217, 686
MluCI	AATT	4 cut(s)	115, 429, 684, 842
Mly113I	GGCGCC	1 cut(s)	294
Mph1103I	ATGCAT	1 cut(s)	856
MroNI	GCCGGC	3 cut(s)	170, 371, 1013
MseI	TTAA	6 cut(s)	105, 414, 483, 536, 708, 840
MslI	CAYNNNNRTG	1 cut(s)	200
MspA1I	CMGCKG	2 cut(s)	140, 328
MspR9I	CCNGG	4 cut(s)	258, 368, 390, 460
Mva1269I	GAATGC	2 cut(s)	759, 1050
MvaI	CCWGG	1 cut(s)	460
MvnI	CGCG	3 cut(s)	32, 123, 306
MwoI	GCNNNNNNNGC	7 cut(s)	117, 180, 325, 809, 833, 864, 905
NaeI	GCCGGC	3 cut(s)	172, 373, 1015
NarI	GGCGCC	1 cut(s)	294
NciI	CCSGG	3 cut(s)	258, 368, 390
NdeI	CATATG	1 cut(s)	829
NdeII	GATC	4 cut(s)	360, 441, 634, 745
NgoMIV	GCCGGC	3 cut(s)	170, 371, 1013
NlaIII	CATG	6 cut(s)	199, 286, 586, 674, 720, 858
NlaIV	GGNNCC	5 cut(s)	69, 136, 295, 550, 991
NsiI	ATGCAT	1 cut(s)	856
NspI	RCATGY	1 cut(s)	858
PaeI	GCATGC	1 cut(s)	858
PcsI	WCGNNNNNNNCGW	2 cut(s)	654, 955
PctI	GAATGC	2 cut(s)	759, 1050
PdiI	GCCGGC	3 cut(s)	172, 373, 1015
PfeI	GAWTC	2 cut(s)	34, 51
Pfl23II	CGTACG	1 cut(s)	471
PflFI	GACNNNGTC	1 cut(s)	245
PluTI	GGCGCC	1 cut(s)	297
Psp6I	CCWGG	1 cut(s)	458
PspGI	CCWGG	1 cut(s)	458
PspLI	CGTACG	1 cut(s)	471
PspN4I	GGNNCC	5 cut(s)	69, 136, 295, 550, 991
PspPI	GGNCC	3 cut(s)	67, 989, 1011
PstNI	CAGNNNCTG	2 cut(s)	328, 465
PsyI	GACNNNGTC	1 cut(s)	245
PvuII	CAGCTG	1 cut(s)	328
RsaI	GTAC	5 cut(s)	136, 473, 550, 948, 961
RsaNI	GTAC	5 cut(s)	135, 472, 549, 947, 960
RseI	CAYNNNNRTG	1 cut(s)	200
SaqAI	TTAA	6 cut(s)	105, 414, 483, 536, 708, 840
Sau3AI	GATC	4 cut(s)	360, 441, 634, 745
Sau96I	GGNCC	3 cut(s)	67, 989, 1011
ScrFI	CCNGG	4 cut(s)	258, 368, 390, 460
SfaNI	GCATC	4 cut(s)	19, 841, 868, 947
SfoI	GGCGCC	1 cut(s)	295
SgrAI	CRCCGGYG	1 cut(s)	290
SinI	GGWCC	2 cut(s)	67, 989
SmiMI	CAYNNNNRTG	1 cut(s)	200
SphI	GCATGC	1 cut(s)	858
Sse9I	AATT	4 cut(s)	115, 429, 684, 842
SsiI	CCGC	6 cut(s)	57, 109, 121, 138, 174, 306
SspDI	GGCGCC	1 cut(s)	293
SspMI	CTAG	4 cut(s)	15, 48, 639, 1040
StyD4I	CCNGG	4 cut(s)	256, 366, 388, 458
StyI	CCWWGG	2 cut(s)	638, 942
TaaI	ACNGT	3 cut(s)	103, 514, 570
TaiI	ACGT	2 cut(s)	246, 489
TaqI	TCGA	2 cut(s)	382, 891
TasI	AATT	4 cut(s)	115, 429, 684, 842
TauI	GCSGC	2 cut(s)	123, 177
TfiI	GAWTC	2 cut(s)	34, 51
Tru1I	TTAA	6 cut(s)	105, 414, 483, 536, 708, 840
Tru9I	TTAA	6 cut(s)	105, 414, 483, 536, 708, 840
TscAI	CASTG	3 cut(s)	106, 451, 517
TseI	GCWGC	9 cut(s)	4, 7, 95, 167, 319, 322, 325, 524, 833
TspDTI	ATGAA	2 cut(s)	218, 224
TspGWI	ACGGA	3 cut(s)	55, 214, 459
TspRI	CASTG	3 cut(s)	106, 451, 517
Tth111I	GACNNNGTC	1 cut(s)	245
VpaK11BI	GGWCC	2 cut(s)	67, 989
XapI	RAATTY	1 cut(s)	842
XceI	RCATGY	1 cut(s)	858
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XmaJI	CCTAGG	1 cut(s)	638
XspI	CTAG	4 cut(s)	15, 48, 639, 1040
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	1 cut(s)	856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.