Prupe.6G212400_v2.0.a1

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014980
Physical Location & Seq
Reverse (-)
22072112 .. 22075285
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G212400.3

Sequence Viewer

Length: 1077 bp
ATGCAGCCTAGCTCGGGAGAAAACGTGAATCAACGGATTGCTAGAATCTCCGCTCATCTCGACCCTCCAAATCTCCACATGGAGAACACTGCCAGTGTCAGCCGCTTGAATTGCCGCGCCAAGGGCGCCGCCGCTGGTTTCAGAGTAGCCGTACTCGGAGCAGCAGGCGGCATAGGACAGCCTCTCGCCATGCTGATGAAGATGAACCCGTTGGTCTCCGTTCTCCATCTGTACGACGTCGTGAACACCCCTGGCGTCACCTCCGATATCAGCCACATGGACACCGGCGCCGTTGTGCGTGGATTTTTGGGGCAGCAGCAGCTGGAGGAAGCGCTGACCGGAGTGGATTTGGTGATCATTCCCGCCGGCGTGCCCCGAAAACCAGGCATGACAAGGGACGATTTGTTCAACATCAATGCCGGAATCGTCAGGACGCTTAGTGAAGCAATCGCTAAGTGCTGTCCAAACGCCATTGTCAACGTGATTAGCAACCCTGTGAACTCCACTGTTCCCATTGCAGCTGAAGTTTTCAAAAAAGCTGGCACCTTTGATCCTAAGAAGCTATTGGGTGTCACCATGCTTGATGTTGTTAGAGCCAATACTTTTGTGGCAGAGGTTCTGGGGCTTGATCCTAGGGACGTTAATGTGCCTGTTGTTGGGGGTCATGCAGGGGTTACAATTTTGCCTCTTTTGTCTCAGGTTAAGCCTCCATGCTCTTTCACCCCAAAAGAAGTTGATTACCTAACAGATCGCATTCAGAATGGTGGAACTGAGGTTGTTGAGGCCAAAGCTGGAGCTGGTTCTGCAACATTATCAATGGCATATGCTGCTGTTAAATTTGCAGATGCATGCCTCCGTGGCTTGCGAGGAGATGCCAGCGTTGTCGAATGTGCATTCGTGGCTTCTCAGGTGACTGAACTTCCCTTCTTTGCATCCAAGGTACGGCTTGGCCGGACTGGAGTTGAGGAAATATACCCTCTTGGCCCCCTGAATGAGTACGAGAGGGCTGGCTTGGAGAGGGCAAAGAAAGAATTAGAATCAAGCATTCAGAAGGGGGTTTCTTTCGTCAGAAAATGA

Protein Analysis

359

Amino Acids

37.82

Weight (kDa)

8.17

Isoelectric Point (pI)

24.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AccB1I GGYRCC 3 cut(s) 125, 287, 542
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 1 cut(s) 117
AciI CCGC 7 cut(s) 51, 103, 115, 129, 132, 168, 363
AclWI GGATC 2 cut(s) 545, 623
AcoI YGGCCR 1 cut(s) 949
AcsI RAATTY 1 cut(s) 836
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 4 cut(s) 126, 237, 255, 288
AfaI GTAC 4 cut(s) 153, 233, 942, 998
AfeI AGCGCT 1 cut(s) 333
AfiI CCNNNNNNNGG 4 cut(s) 14, 121, 656, 942
AgsI TTSAA 3 cut(s) 109, 409, 532
AjnI CCWGG 2 cut(s) 250, 382
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 7 cut(s) 12, 322, 521, 539, 562, 791, 797
AluI AGCT 7 cut(s) 12, 322, 521, 539, 562, 791, 797
Alw26I GTCTC 2 cut(s) 220, 699
AlwI GGATC 2 cut(s) 545, 623
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 13
Aor51HI AGCGCT 1 cut(s) 333
AoxI GGCC 3 cut(s) 783, 949, 982
ApeKI GCWGC 7 cut(s) 4, 161, 313, 316, 319, 518, 827
ApoI RAATTY 1 cut(s) 836
AspA2I CCTAGG 1 cut(s) 632
AspLEI GCGC 4 cut(s) 119, 128, 290, 334
AspS9I GGNCC 1 cut(s) 983
AsuHPI GGTGA 5 cut(s) 250, 364, 565, 712, 922
AvaI CYCGRG 1 cut(s) 13
AvrII CCTAGG 1 cut(s) 632
BaeGI GKGCMC 1 cut(s) 375
BanI GGYRCC 3 cut(s) 125, 287, 542
BbvI GCAGC 7 cut(s) 16, 173, 325, 328, 331, 530, 814
BccI CCATC 1 cut(s) 234
BceAI ACGGC 3 cut(s) 134, 275, 959
BciT130I CCWGG 2 cut(s) 252, 384
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 2 cut(s) 220, 699
BfaI CTAG 3 cut(s) 9, 42, 633
BfoI RGCGCY 3 cut(s) 129, 291, 335
BglI GCCNNNNNGGC 1 cut(s) 858
BlnI CCTAGG 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 252, 384
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 983
BmiI GGNNCC 4 cut(s) 127, 289, 544, 985
BmrFI CCNGG 2 cut(s) 252, 384
BmsI GCATC 3 cut(s) 835, 862, 941
BpmI CTGGAG 3 cut(s) 344, 813, 978
BsaHI GRCGYC 4 cut(s) 126, 237, 255, 288
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 5 cut(s) 120, 250, 632, 856, 936
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 121, 656, 942
Bse118I RCCGGY 2 cut(s) 284, 365
Bse1I ACTGG 2 cut(s) 93, 961
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 2 cut(s) 252, 384
BseDI CCNNGG 5 cut(s) 120, 250, 632, 856, 936
BseGI GGATG 1 cut(s) 932
BseLI CCNNNNNNNGG 4 cut(s) 14, 121, 656, 942
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 3 cut(s) 710, 762, 920
BseNI ACTGG 2 cut(s) 93, 961
BseRI GAGGAG 1 cut(s) 882
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 7 cut(s) 16, 173, 325, 328, 331, 530, 814
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 3 cut(s) 785, 951, 984
BshNI GGYRCC 3 cut(s) 125, 287, 542
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 5 cut(s) 285, 339, 366, 420, 952
BslFI GGGAC 2 cut(s) 410, 650
BslI CCNNNNNNNGG 4 cut(s) 14, 121, 656, 942
BsmAI GTCTC 2 cut(s) 220, 699
BsmFI GGGAC 2 cut(s) 410, 650
BsmI GAATGC 3 cut(s) 753, 893, 1044
BsnI GGCC 3 cut(s) 785, 951, 984
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 375
Bsp143I GATC 4 cut(s) 354, 550, 628, 748
BspACI CCGC 7 cut(s) 51, 103, 115, 129, 132, 168, 363
BspANI GGCC 3 cut(s) 785, 951, 984
BspCNI CTCAG 3 cut(s) 709, 763, 919
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 4 cut(s) 127, 289, 544, 985
BspPI GGATC 2 cut(s) 545, 623
BspT107I GGYRCC 3 cut(s) 125, 287, 542
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 2 cut(s) 284, 365
BsrI ACTGG 2 cut(s) 93, 961
BssAI RCCGGY 2 cut(s) 284, 365
BssECI CCNNGG 5 cut(s) 120, 250, 632, 856, 936
BssMI GATC 4 cut(s) 354, 550, 628, 748
BssNI GRCGYC 4 cut(s) 126, 237, 255, 288
BssT1I CCWWGG 3 cut(s) 120, 632, 936
Bst2UI CCWGG 2 cut(s) 252, 384
Bst4CI ACNGT 1 cut(s) 508
BstACI GRCGYC 4 cut(s) 126, 237, 255, 288
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 8 cut(s) 166, 367, 371, 541, 850, 863, 877, 1009
BstDEI CTNAG 6 cut(s) 437, 453, 555, 696, 771, 906
BstDSI CCRYGG 1 cut(s) 856
BstF5I GGATG 1 cut(s) 932
BstFNI CGCG 1 cut(s) 117
BstH2I RGCGCY 3 cut(s) 129, 291, 335
BstHHI GCGC 4 cut(s) 119, 128, 290, 334
BstKTI GATC 4 cut(s) 357, 553, 631, 751
BstMAI GTCTC 2 cut(s) 220, 699
BstMBI GATC 4 cut(s) 354, 550, 628, 748
BstMWI GCNNNNNNNGC 8 cut(s) 111, 123, 125, 319, 803, 827, 858, 899
BstNI CCWGG 2 cut(s) 252, 384
BstNSI RCATGY 1 cut(s) 852
BstSCI CCNGG 2 cut(s) 250, 382
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 7 cut(s) 16, 173, 325, 328, 331, 530, 814
BsuRI GGCC 3 cut(s) 785, 951, 984
BtgI CCRYGG 1 cut(s) 856
BtsCI GGATG 1 cut(s) 932
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 100, 504
Cac8I GCNNGC 8 cut(s) 166, 367, 371, 541, 850, 863, 877, 1009
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 4 cut(s) 119, 128, 290, 334
Cfr10I RCCGGY 2 cut(s) 284, 365
Cfr13I GGNCC 1 cut(s) 983
CseI GACGC 2 cut(s) 244, 442
Csp6I GTAC 4 cut(s) 152, 232, 941, 997
CviAII CATG 8 cut(s) 79, 190, 277, 388, 577, 665, 711, 849
CviQI GTAC 4 cut(s) 152, 232, 941, 997
DdeI CTNAG 6 cut(s) 437, 453, 555, 696, 771, 906
DinI GGCGCC 2 cut(s) 127, 289
DpnI GATC 4 cut(s) 356, 552, 630, 750
DpnII GATC 4 cut(s) 354, 550, 628, 748
EaeI YGGCCR 1 cut(s) 949
Eco130I CCWWGG 3 cut(s) 120, 632, 936
Eco31I GGTCTC 1 cut(s) 220
Eco32I GATATC 1 cut(s) 268
Eco47III AGCGCT 1 cut(s) 333
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 13
EcoRII CCWGG 2 cut(s) 250, 382
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 3 cut(s) 120, 632, 936
EcoT22I ATGCAT 1 cut(s) 850
EgeI GGCGCC 2 cut(s) 127, 289
EheI GGCGCC 2 cut(s) 127, 289
ErhI CCWWGG 3 cut(s) 120, 632, 936
FaeI CATG 8 cut(s) 82, 193, 280, 391, 580, 668, 714, 852
FaqI GGGAC 2 cut(s) 410, 650
FatI CATG 8 cut(s) 78, 189, 276, 387, 576, 664, 710, 848
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 823
FbaI TGATCA 1 cut(s) 354
FokI GGATG 1 cut(s) 919
FspBI CTAG 3 cut(s) 9, 42, 633
GlaI GCGC 4 cut(s) 118, 127, 289, 333
GsuI CTGGAG 3 cut(s) 344, 813, 978
HaeII RGCGCY 3 cut(s) 129, 291, 335
HaeIII GGCC 3 cut(s) 785, 951, 984
HapII CCGG 5 cut(s) 285, 339, 366, 420, 952
HgaI GACGC 2 cut(s) 244, 442
HhaI GCGC 4 cut(s) 119, 128, 290, 334
Hin1I GRCGYC 4 cut(s) 126, 237, 255, 288
Hin1II CATG 8 cut(s) 82, 193, 280, 391, 580, 668, 714, 852
Hin6I GCGC 4 cut(s) 117, 126, 288, 332
HinP1I GCGC 4 cut(s) 117, 126, 288, 332
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 4 cut(s) 28, 45, 423, 1037
HpaII CCGG 5 cut(s) 285, 339, 366, 420, 952
HphI GGTGA 5 cut(s) 250, 364, 565, 712, 922
Hpy166II GTNNAC 3 cut(s) 244, 478, 499
Hpy188I TCNGA 6 cut(s) 143, 158, 265, 759, 1050, 1070
Hpy188III TCNNGA 4 cut(s) 15, 59, 241, 430
Hpy8I GTNNAC 3 cut(s) 244, 478, 499
Hpy99I CGWCG 2 cut(s) 239, 242
HpyAV CCTTC 2 cut(s) 934, 1045
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 4 cut(s) 24, 237, 480, 639
HpyCH4V TGCA 8 cut(s) 4, 518, 668, 806, 842, 848, 893, 932
HpyF10VI GCNNNNNNNGC 8 cut(s) 111, 123, 125, 319, 803, 827, 858, 899
HpyF3I CTNAG 6 cut(s) 437, 453, 555, 696, 771, 906
HpySE526I ACGT 4 cut(s) 24, 237, 480, 639
Hsp92I GRCGYC 4 cut(s) 126, 237, 255, 288
Hsp92II CATG 8 cut(s) 82, 193, 280, 391, 580, 668, 714, 852
HspAI GCGC 4 cut(s) 117, 126, 288, 332
KasI GGCGCC 2 cut(s) 125, 287
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 4 cut(s) 354, 550, 628, 748
LmnI GCTCC 2 cut(s) 158, 794
Lsp1109I GCAGC 7 cut(s) 16, 173, 325, 328, 331, 530, 814
LweI GCATC 3 cut(s) 835, 862, 941
MaeI CTAG 3 cut(s) 9, 42, 633
MaeII ACGT 4 cut(s) 24, 237, 480, 639
MaeIII GTNAC 4 cut(s) 256, 571, 673, 910
MalI GATC 4 cut(s) 356, 552, 630, 750
MbiI CCGCTC 1 cut(s) 53
MboI GATC 4 cut(s) 354, 550, 628, 748
MboII GAAGA 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 4 cut(s) 109, 678, 836, 1031
Mly113I GGCGCC 2 cut(s) 126, 288
Mph1103I ATGCAT 1 cut(s) 850
MreI CGCCGGCG 1 cut(s) 365
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 3 cut(s) 642, 702, 834
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 3 cut(s) 134, 322, 521
MspI CCGG 5 cut(s) 285, 339, 366, 420, 952
MspR9I CCNGG 2 cut(s) 252, 384
Mva1269I GAATGC 3 cut(s) 753, 893, 1044
MvaI CCWGG 2 cut(s) 252, 384
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 8 cut(s) 111, 123, 125, 319, 803, 827, 858, 899
NaeI GCCGGC 1 cut(s) 367
NarI GGCGCC 2 cut(s) 126, 288
NdeI CATATG 1 cut(s) 823
NdeII GATC 4 cut(s) 354, 550, 628, 748
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 8 cut(s) 82, 193, 280, 391, 580, 668, 714, 852
NlaIV GGNNCC 4 cut(s) 127, 289, 544, 985
NmuCI GTSAC 3 cut(s) 256, 571, 910
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 1 cut(s) 852
PaeI GCATGC 1 cut(s) 852
PctI GAATGC 3 cut(s) 753, 893, 1044
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 4 cut(s) 28, 45, 423, 1037
PluTI GGCGCC 2 cut(s) 129, 291
Psp6I CCWGG 2 cut(s) 250, 382
PspGI CCWGG 2 cut(s) 250, 382
PspN4I GGNNCC 4 cut(s) 127, 289, 544, 985
PspPI GGNCC 1 cut(s) 983
PstNI CAGNNNCTG 1 cut(s) 322
PvuII CAGCTG 2 cut(s) 322, 521
RsaI GTAC 4 cut(s) 153, 233, 942, 998
RsaNI GTAC 4 cut(s) 152, 232, 941, 997
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 642, 702, 834
Sau3AI GATC 4 cut(s) 354, 550, 628, 748
Sau96I GGNCC 1 cut(s) 983
ScrFI CCNGG 2 cut(s) 252, 384
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 3 cut(s) 835, 862, 941
SfoI GGCGCC 2 cut(s) 127, 289
SgrAI CRCCGGYG 2 cut(s) 284, 365
SmiMI CAYNNNNRTG 1 cut(s) 194
SphI GCATGC 1 cut(s) 852
Sse9I AATT 4 cut(s) 109, 678, 836, 1031
SsiI CCGC 7 cut(s) 51, 103, 115, 129, 132, 168, 363
SspDI GGCGCC 2 cut(s) 125, 287
SspMI CTAG 3 cut(s) 9, 42, 633
StyD4I CCNGG 2 cut(s) 250, 382
StyI CCWWGG 3 cut(s) 120, 632, 936
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 4 cut(s) 27, 240, 483, 642
TaqI TCGA 2 cut(s) 60, 885
TasI AATT 4 cut(s) 109, 678, 836, 1031
TauI GCSGC 5 cut(s) 105, 117, 131, 134, 171
TfiI GAWTC 4 cut(s) 28, 45, 423, 1037
Tru1I TTAA 3 cut(s) 642, 702, 834
Tru9I TTAA 3 cut(s) 642, 702, 834
TscAI CASTG 3 cut(s) 94, 100, 511
TseFI GTSAC 3 cut(s) 256, 571, 910
TseI GCWGC 7 cut(s) 4, 161, 313, 316, 319, 518, 827
Tsp45I GTSAC 3 cut(s) 256, 571, 910
TspDTI ATGAA 2 cut(s) 212, 218
TspGWI ACGGA 3 cut(s) 49, 208, 845
TspRI CASTG 3 cut(s) 94, 100, 511
XapI RAATTY 1 cut(s) 836
XceI RCATGY 1 cut(s) 852
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmaJI CCTAGG 1 cut(s) 632
XspI CTAG 3 cut(s) 9, 42, 633
ZraI GACGTC 1 cut(s) 238
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.